International Journal of Polymeric Materials and Polymeric Biomaterials
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2019
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IF 2.263
Yajing Pan,Chao Xiao,Huaping Tan,Guoliang Yuan,Jianliang Li,Shengke Li,Yang Jia,Dangsheng Xiong,Xiaohong Hu,Xiaohong Niu
Microspheres and injectable hydrogels derived from natural biopolymers have been extensively investigated as drug carriers and cell scaffolds. In this study, we report a preparation of composite scaffolds basing microspheres and hydrogel via the Schiff’s base reaction. Hybrid gelatin/heparin microspheres loading insulin-like growth factor-1 (IGF-1) with a diameter of 5–10 µm were fabricated using an emulsion cross-linking method. Synchronously, water-soluble carboxymethyl chitosan (CMC) and oxidized chondroitin sulfate (OCS) were prepared for cross-linking of hydrogels, which were embedded with microspheres to produce a composite microspheres/gel scaffold. The mechanism of scaffold cross-linking is attributed to the Schiff’s base reaction between amino and aldehyde groups of biopolymers. Currently, gelation rate, morphology, mechanical properties, swelling ratio, weight loss, and IGF-1 release of the composite scaffolds were examined in vitro. The results show that mechanical and bioactive properties of CMC-OCS hydrogel can be significantly improved by embedding gelatin/heparin microspheres containing IGF-1. Compressive modulus of composite gel scaffolds containing 3 wt% of microspheres was 16 kPa, which was higher than the control hydrogel without microspheres. Cumulative release of IGF-1 during 7 days from microspheres embedded hydrogel was 70%, which was significantly lower than those of microspheres and hydrogels. Moreover, the composite microspheres/gel scaffolds exhibited higher swelling ratio and slower degradation rate than the control. Potential of the composite scaffolds was demonstrated by encapsulation of human adipose-derived stem cells (ASCs) in vitro. Cell culture showed that this composite hydrogel could support survival and proliferation of ASCs. These results demonstrate the potential of gelatin/heparin microspheres embedded CMC-OCS hydrogels as an injectable scaffold in soft tissue engineering.
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CARBOHYDRATE POLYMERS
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2019
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IF 6.044
Hu Tu,Guomin Wu,Yang Yi,Mengtian Huang,Rong Liu,Xiaowen Shi,Hongbing Deng
Novel antimicrobials with well biocompatibility are urgently needed for human public health protecting. Here, the silk fibroin (SF) nanofibrous mats coated with carboxymethyl chitosan (CMC), both extracted from natural polymers, were fabricated by combining electrospinning and electrostatic layer-by-layer (LBL) self-assembly techniques. The amphoteric CMC could be easily adsorbed on the surface of SF nanofibers due to the electrostatic interaction, which were a highly efficient and “green” route for the surface modification of SF mats. The mats after LBL procedure exhibited better hydrophilicity and stronger mechanical properties. The experimental results demonstrated that the LBL modified mats not only kept well biocompatibility but also obtained extremely enhanced antibacterial activity. More importantly, the mats displayed better bacterial inhibition with the increased CMC layers. LBL 10 and LBL 10.5 owned the antibacterial activity of more than 67% at the bacterial concentrations of 10 6 cfu ml −1 after 24 h cultivation, which implied that these novel natural polymer-based materials could be utilized as wound dressings for clinical skin and tissue regeneration, especially for infected wounds.
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CHEMICAL RESEARCH IN CHINESE UNIVERSITIES
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2019
·Vol. 35
·期 3
·504-508
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IF 1.071
Cao Jing,Wang Miao,Chen Weihua,She Yongxin,Wang Jing,Wang Fengzhong,Lao Shuibing
A novel artificial esterase based on chitosan and amino acids was synthesized in the present study. The Fmoc-His and Glu were linked to chitosan by active ester method(AEM). The hydroxide radical in chitosan, imidazole group of Fmoc-His and carboxyl from Glu formed a catalytic center of natural esterase. Gel microspheres were coated with a protective layer and a supporting layer by self-assembly construction function in carboxymethylcellulose sodium(CMCS) solution. As for catalytic activity, chitosan-His-Glu was found to be more efficient than chitosan- His and chitosan-Glu in mimicking the core catalytic sites of natural esterase, and the best ratio(mass ratio) of chitosan-His-Glu:CMCS was 1:3. Furthermore, metal ions, such as Ca 2+ , Mg 2+ , Fe 2+ , etc., were able to improve the catalytic efficiency of artificial esterase. And the Lineweaver-Burk plot indicated that the catalytic kinetics of artificial esterase conformed to Michaelis-Menten equation.
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COLLOIDS AND SURFACES B-BIOINTERFACES
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2019
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IF 3.973
Hui Wang,Ziming Yang,Zuyu He,Chuang Zhou,Chao Wang,Yu Chen,Xianwu Liu,Sidong Li,Puwang Li
Quercetin (QCT) has important functions such as antioxidant, anti-inflammatory and anticancer. However, its applications in food and in drug are restricted owing to its poor water solubility. In this work, a novel amphiphilic wall-material chitosan was synthesized via grafting of chitosan with deoxycholic acid (DA) as hydrophobic group and modified N-acetyl-L-cysteine (NAC) as hydrophilic group. Amphiphilic chitosan was self-assembled to load QCT as nanomicelles by a low-cost and inorganic solvent procedure. Both the encapsulation efficiencies (EE) and drug-loading rates (DL) increased when increasing the grafting rate of DA. There was a bursting release of QCT for the QCT-loaded nanomicelles (CS-DA-NAC-QNMs) from 0 to 8 h, and then the release rate decreased gradually. After releasing for 72 h, the final cumulative release percentages were more than 40%. All the QCT-loaded nanomicelles samples showed good hemocompatibility, and their water solubility and biocompatibility increased evidently. What’s more, they exhibited an obvious inhibition rate of A549 cells.
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JOURNAL OF APPLIED POLYMER SCIENCE
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2019
·Vol. 136
·期 22
·47603
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IF 2.188
Saad Ahmed,Muhammad Ali,Yangben Cai,Yunhua Lu,Zaheer Ahmad,Santosh Khannal,Shiai Xu
In the present study, multi-walled carbon nanotubes (MWCNTs) were sulfonated by 1,3-propane sultone and distillation–precipitation polymerization, respectively, and then incorporated into chitosan (CS) to prepare CS/MWCNTs composite membranes for fuel cell applications. CS/MWCNTs membranes show better thermal and mechanical stability than pure CS membrane due to the strong electrostatic interaction between the SO 3 H groups of MWCNTs and the NH 2 groups of CS, which can restrict the mobility of CS chain. The sulfonated MWCNTs provide efficient proton hopping sites ( SO 3 H, SO 3 − …. 3 + HN ), thereby resulting in the formation of continuous proton conducting channels. The composite membranes with 5 wt % of MWCNTs modified by two different ways show a proton conductivity of 0.026 and 0.025 S·cm −1 , respectively. In conclusion, CS/MWCNTs membrane is a promising proton exchange membrane for fuel-cell applications. © 2019 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2019 , 136 , 47603.
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Journal of Nanomaterials
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2019
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IF 2.233
Xiaojuan Zhang,Wenbin Cai,Lingyun Hao,Wei Zhang
This study synthesized poly(vinyl alcohol)/carboxymethyl chitosan/cyanidin (PVA/CMCS/CY) hydrogel films. First, PVA and CMCS were used to synthesize hydrogel films by ultraviolet irradiation. Meanwhile, CY was in situ combined into the hydrogel films through the electrostatic attraction between CMCS and CY. Next, the products were analyzed using Fourier-transform infrared spectroscopy, scanning electron microscopy, contact angle test, swelling analysis, and mechanical property test. The results revealed that compared with PVA/CMCS hydrogel films, the PVA/CMCS/CY hydrogel films had an interporous structure and good swelling and mechanical properties. Moreover, the drug release experiments demonstrated that the PVA/CMCS/CY hydrogel films had a CY encapsulation efficiency of 33.5% with a sustained CY release of up to 60 h. Furthermore, the examined antibacterial activities against Staphylococcus aureus and Escherichia coli showed that the porous PVA/CMCS/CY hydrogel films exhibited a certain inhibition. Cell viability experiments demonstrated that the PVA/CMCS/CY films displayed no obvious cytotoxicity to human umbilical vein endothelial cells.
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2019
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IF 4.784
Jibo Dou,Defu Gan,Qiang Huang,Meiying Liu,Junyu Chen,Fengjie Deng,Xiaoli Zhu,Yuanqing Wen,Xiaoyong Zhang,Yen Wei
In this work, we reported a novel “one-pot” strategy for preparation of chitosan-coated carbon nanotubes (CNTs) composites via a combination of Diels-Alder (DA) reaction and mercaptoacetic acid locking imine (MALI) reaction for the first time. To evaluate the adsorption characteristics, the as-prepared samples were applied to remove copper ions (Cu 2+ ) from aqueous solution. The effects of contact time, solution pH, temperature and initial Cu 2+ concentration on the adsorption of Cu 2+ onto the as-prepared samples were investigated. The chitosan modified CNTs composites showed high affinity and fast kinetics for the adsorption of Cu 2+ ions, and adsorption capacity of the composites was found to be 2 times that of pristine CNTs. Adsorption kinetics and thermodynamic indicated a spontaneous and endothermic nature of the adsorption of Cu 2+ on the surface of chitosan-coated CNTs composites, kinetically obeyed the pseudo-second-order model. Equilibrium data could be best described by the Langmuir isotherm model, with a maximum monolayer adsorption capacity of 115.84 mg/g. In view of the extensive applicability of DA chemistry and MALI reaction, different carbon nanomaterials based composites with various functional groups could be fabricated and applicable to different fields such as environmental catalysis and biomedicine .
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Clinical Implant Dentistry and Related Research
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2019
·Vol. 21
·期 2
·324-335
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IF 3.212
Xiaoxiao Cao,Xinjie Cai,Ruiying Chen,Huimei Zhang,Tao Jiang,Yining Wang
Background Mechanical and biological complications associated with implant systems happen frequently in the clinic. Purpose To develop a chitosan (CS)-based thermosensitive hydrogel for sealing and lubricating purposes in dental implant system. Materials and Methods In this study, a thermosensitive hydrogel made up of CS, β-glycerophosphate pentahydrate (β-GP), and povidone-iodine (PVP-I), called CS/β-GP/PVP-I thermosensitive hydrogel, was fabricated. Three experimental groups with different volume ratios of CS to β-GP were prepared, namely 16/4, 13/7, and 10/10 groups. The surface topography of the different groups and their physicochemical characteristics were examined by SEM, FTIR, and X-ray diffraction analysis. The cytotoxicity of the hydrogel was examined by CCK-8 test. In vitro antibacterial efficiency was analyzed by the spread plate method. Sealing ability was detected by incubating two-piece implants in Escherichia coli suspension. Lubricating ability of the hydrogel was evaluated by the removal torque test with a calibrated digital torque meter. Results The CS/β-GP/PVP-I thermosensitive hydrogel was fabricated and showed a highly porous structure under SEM. An in vitro cytotoxicity test demonstrated that 13/7 group displayed no cytotoxicity. Furthermore, all three groups showed obviously antibacterial effects. In the sealing ability test, 16/4 group showed the best sealing ability. The removal torque of 16/4 group and 13/7 group was significantly greater than control group. Conclusions Based on our findings, it could be concluded that the thermosensitive and antibacterial CS/β-GP/PVP-I hydrogel with sealing and lubricating ability was successfully prepared. The hydrogel had better sealing and lubricating effects when the volume ratio of CS to β-GP was 16/4.
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CHEMICAL ENGINEERING SCIENCE
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2019
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IF 3.372
Haiyan Zhang,Chunjie Liu,Liang Chen,Bin Dai
We presented an improved preparation method to generate cryogels with controllable pore size. It included 5 stages: pre-cooling, pre-crystallization, crystal growth, freezing, and thawing (PPCFT); crystal growth was the key step. Chitosan (CS) cryogels with uniform and honeycomb-like structures were prepared with the method, and then the properties of these cryogels were studied. The results demonstrated that the mean pore diameters of CS cryogels were successfully controlled in a wide range (approximately 60–240 μm, by scanning electron microscope) by simply varying the temperature and time of crystal growth. The crystal growth law of this process was described by an empirical equation based on the classical theory for Ostwald ripening by Lifshitz, Slyozov, and Wagner (LSW). Furthermore, the hydrodynamics performance (permeability and column efficiency) of CS cryogel columns clearly varied with crystal growth conditions, while both the accessible porosity and water absorption ratio remained at a high level.
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PROGRESS IN ORGANIC COATINGS
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2019
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IF 3.42
Bingyang Lu,Dan Luo,Ansha Zhao,Haohao Wang,Yuancong Zhao,Manfred F. Maitz,Ping Yang,Nan Huang
Environmentally responsive polymer films have received much attention, but the stability of such films and the sensitivity of the response still have to be improved. The preparation of responsive films for low pH changes still faces challenges. In this study, catechol groups were used to functionalize chitosan and hyaluronic acid for mussel-inspired, catechol based adhesion. The modified chitosan and the modified hyaluronic acid were deposited in a layer-by-layer manner. The chemical properties of the compounds and surface properties of the coatings were characterized by UV, IR, scanning electron microscopy and water contact angles. The assembly and stability of the film were investigated in situ using quartz crystal microbalance (QCM-D). The film had improved stability compared to the native polymers and it showed reversible pH-dependent swelling and shrinkage. The film was loaded with a drug and placed in PBS solutions of different pH for several days, and the amount of drug released by the drug-loaded film was different under different pH environments. This provides ideas for the intelligent application in materials science and biology, and has broad prospects in drug delivery systems and tissue engineering.
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2019
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IF 4.784
Shuting Li,Liefeng Hu,Dapeng Li,Xin Wang,Panpan Zhang,Jun Wang,Guoqing Yan,Rupei Tang
This work described the preparation of two type nanogels based on the crosslinking between carboxymethyl chitosan (CMCS) and two different crosslink agents, an acid-labile cyclic ortho ester compound with dual epoxy end groups (OEDe) or corresponding non-sensitive ethyleneglycol diglycidyl ether (EGDE). The particle size, zeta potential, and micromorphology were characterized by dynamic light scattering and electron microscopy, respectively. Nanogels' stability was also investigated at physiological environments. Doxorubicin hydrochloride as a therapeutic drug model was efficiently embedded into nanogels. The pH-triggered size changing, degradation and drug release were then investigated at three different pH values. Cellular uptake and cytotoxicity evaluation demonstrated that NG1/DOX could be successfully degraded and efficiently release DOX in acid cell organelles, leading to higher cytotoxicity than NG2/DOX. The accumulation and penetration of these DOX-loaded nanogel were then investigated by tumor-like multicellular spheroids (MCTS). The results indicated that the acid-degradable nanogels can deliver more DOX into the inner of MCTS by the hydrolysis of ortho ester bonds, thus efficiently inhibit the growth of MCTS. All results suggested that the acid-degradable nanogels could be degraded in mildly acidic conditions and remain stable at physiological environment, which indicated that the acid-degradable nanogels would be potentially useful as drug carriers.
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FOOD AND CHEMICAL TOXICOLOGY
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2019
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IF 3.775
Mingjing Zheng,Suzhen Lei,Hongqiang Wu,Baodong Zheng,Yi Zhang,Hongliang Zeng
The effect of chitosan (CS) on the in vitro digestibility and molecular structural properties of lotus seed starch (LS), and the correlation matrix was studied. The addition of CS could delay the hydrolysis of LS due to a increased level of slowly digestible starch (SDS). LS-CS blends exhibited lower pasting viscosity, greater amylose content and higher ordered structure than LS alone. A significant correlation was found between the digestibility and molecular structural properties of LS-CS blends. Rapidly digestible starch content was positively correlated with viscosity and full width at half-maximum height (FWHH) at 480 cm −1 ; whereas, SDS content was negatively correlated with setback and FWHH. Moreover, CS concentration was positively related to absorbances at 1047 and 1035 cm −1 and amylose content. The results indicated that the addition of CS could be beneficial to the formation of an ordered molecular structure in LS-CS blends and decreased digestibility in vitro .
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