JOURNAL OF FOOD COMPOSITION AND ANALYSIS
·
2025
·
IF 4.6
Mintong Tian,Huan Gao,Purui Yu,Mingming Li,Zehua Li,Qipeng Yuan
Sulforaphane (SFA), an isothiocyanate compound derived from cruciferous plants, has garnered significant attention owing to its cancer-preventive, anti-inflammatory, and antibacterial properties. However, the presence of complex impurities, including hydrolysis by-products of glucosinolates, structural analogues of SFA, and 5-vinyl-2-thioxazolidinone (VOT), in plant extracts significantly hinders both the efficiency of purification and the safety of application. In this study, 14 compounds were successfully isolated and identified from broccoli seed extracts using preparative high-performance liquid chromatography. Additionally, an efficient separation method was developed for removing the crucial impurity VOT based on the temperature-responsive properties of β-cyclodextrin (β-CD). The results demonstrated that the selective binding of β-CD to SFA at elevated temperatures achieved a VOT removal efficiency exceeding 97% following low-temperature precipitation. Additionally, the purity of SFA was enhanced from 55.8% to 90.1%. The separation mechanism at the molecular level was validated through density functional theory calculations. The binding energy of β-CD-SFA (-81.2 kJ/mol) was significantly higher than that of β-CD-VOT (-25.6 kJ/mol). The scanning electron microscopy and energy dispersive spectroscopy results confirmed the homogeneous distribution of SFA within the complexes. This study provides an innovative approach for the large-scale preparation of high-purity SFA and ensures its safe application in functional foods.
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高影响因子
Polymers
·
2021
·Vol. 13
·期 16
·2759
·
IF 4.329
Longwei Jiang,Yanlong Han,Xiangyi Meng,Yawen Xiao,Huajiang Zhang
In this study, following the green, environmentally friendly and sustainable development strategy, cellulose nanocrystals (CNCs) were prepared through a solvent-free esterification reaction between microcrystalline cellulose and maleic anhydride, combined with subsequent ultrasonic treatment, and maleic-anhydride-modified CNC-reinforced zein/catechin/β-cyclodextrin inclusion complex nanoparticles nanocomposite films were prepared by a facile solution casting. The amount of CNCs in the film matrix was 0–8 wt%, and their effect on structural, physicochemical and functional properties of the resulting films were investigated. SEM images showed that the addition of CNCs made the microstructure of the film more smooth and uniform. The intermolecular hydrogen bonds between CNCs and film matrix were supported by FT-IR. XRD analysis also confirmed the appearance of a crystalline peak due to the existence of CNCs inside the films. The incorporation of CNCs significantly reduced water vapor permeability, water solubility and the swelling degree of the nanocomposite film, and also significantly increased tensile strength and elongation at break from 12.66 to 37.82 MPa and 4.5% to 5.2% (p< 0.05). Moreover, nanocomposite film packaging with CNCs can effectively inhibit the oxidation of soybean oil.Keywords:cellulose nanocrystals;zein;antioxidant activity;biodegradable films;food oxidation
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高影响因子
PEST MANAGEMENT SCIENCE
·
2021
·Vol. 77
·期 7
·3224-3232
·
IF 4.845
Xi-le Deng,Lei Guo,Hai-hao Ma,Xue-ping Hu,Xiao-mao Zhou
BACKGROUND The diamondback moth ( Plutella xylostella ) is one of the most destructive lepidopteran pests on cruciferous vegetables. However, resistance has emerged to current chemical and biological insecticides used for P. xylostella control, indicating the necessity of screening new targets on P. xylostella , and finding new insecticides against P. xylostella . In particular, octopamine receptors are representative G protein-coupled receptors found only in invertebrates and are potential targets for identifying novel insecticides. RESULTS A β-adrenergic-like octopamine receptor gene ( PxOA2B1 ) was cloned, and its pharmacological characteristics in P. xylostella were studied. The results demonstrated that octopamine could activate the PxOA2B1 receptor, with a half-maximal effective concentration (EC 50 ) of 49.5 n m . Amitraz, an insecticide and acaricide, and its metabolite ( N -2,4-dimethylphenyl- N ′-methylformamidine; DPMF) were also found to act as PxOAB1R agonists. We synthesized phenyl imidazolidin-2-one derivatives 3a–h using DPMF as the lead compound, and compounds 3a–h showed similar antagonist activities as phentolamine, mianserin and chlorpromazine. In particular, 3d , with an EC 50 of 25.2 n m , showed very similar antagonist activity to mianserin. CONCLUSION This research found that PxOAB1R might be a potential target for P. xylostella control. Phenyl imidazolidin-2-ones could be novel potential antagonists targeted at octopamine receptors and would be useful tools for the design and development of novel insecticides. © 2021 Society of Chemical Industry
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高影响因子
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
·
2022
·Vol. 106
·期 24
·8053-8066
·
IF 5.56
Li Meijie,Wen Qifeng,Lv Shuzhe,Yang Rumeng,Cheng Tao,Wang Zhaobao,Yang Jianming
Abstract
β-Elemene is the major component of a traditional Chinese medicine ( Rhizoma Curcumae ) for cancer treatment, and plant extraction is the major methods currently. Biosynthesis of β-elemene is a promising and attractive route due to its advantages, including environmentally friendly processes, renewable resources, and sustainable development. In this research, biosynthesis of germacrene A, direct precursor of β-elemene, in Escherichia coli was successfully performed and 11.99 mg/L germacrene A was obtained. Thereafter, a cobiosynthesis system for germacrene A and lycopene, another kind of isoprenoid, was constructed. Furthermore, the cultivation conditions were optimized. The germacrene A production was increased to the highest level reported to date, 364.26 mg/L, threefold increase to the strain with only germacrene A production. The cobiosynthesis system was suggested to promote the metabolic flux for germacrene A production. This research enabled germacrene A production in E. coli , and it highlights the promoting mechanism of the cobiosynthesis system for two chemicals which are both belonging to isoprenoids. Key points
• Co-production of germacrene A and lycopene in E. coli. • Promoting mechanism of cobiosynthesis of two isoprenoid compounds in E. coli. • Shake-flask production of germacrene A reached to the highest 364.26 mg/L in E. coli.
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高影响因子
Theranostics
·
2022
·Vol. 12
·期 15
·6646-6664
·
IF 11.6
Ni Liu,Xiaohan Liang,Changwen Yang,Shun Hu,Qingming Luo,Haiming Luo
Rationale: Active removal of excess peripheral amyloid-β (Aβ) can potentially treat Alzheimer's disease (AD). However, the peripheral clearance of Aβ using an anti-Aβ monoclonal antibody (mAb) cannot remove PET-detectable Aβ within the brain. This may be due to the inability of mAb to cross the blood-brain barrier (BBB) to degrade insoluble brain Aβ plaques and block liver dysfunction. Methods: We developed a dual-targeted magnetic mesoporous silica nanoparticle (HA-MMSN-1F12) through surface-coupled Aβ 42 -targeting antibody 1F12 and CD44-targeting ligand hyaluronic acid (HA). Results: HA-MMSN-1F12 had a high binding affinity toward Aβ 42 oligomers (Kd = 1.27 ± 0.34 nM) and revealed robust degradation of Aβ 42 aggregates. After intravenous administration of HA-MMSN-1F12 into ten-month-old APP/PS1 mice for three weeks (4 mg/kg/week), HA-MMSN-1F12 could cross the BBB and depolymerize brain Aβ plaques into soluble Aβ species. In addition, it also avoided hepatic uptake and excreted captured Aβ species through intestinal metabolism, thereby reducing brain Aβ load and neuroinflammation and improving memory deficits of APP/PS1 mice. Furthermore, the biochemical analysis showed that HA-MMSN-1F12 did not detect any toxic side effects on the liver and kidney. Thus, the efficacy of HA-MMSN-1F12 is associated with the targeted degradation of insoluble brain Aβ plaques, avoidance of non-specific hepatic uptake, and excretion of peripheral Aβ through intestinal metabolism. Conclusions: The study provides a new avenue for treating brain diseases by excreting disease-causing biohazards using intestinal metabolism.
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INTERNATIONAL JOURNAL OF PHARMACEUTICS
·
2009
·
IF 3.061
Jianyu Xin,Zhizhang Guo,Xingyu Chen,Wenfeng Jiang,Jianshu Li,Maolin Li
A series of branched cationic β-cyclodextrin polymers (CPβCDs) with designed chemical structures were synthesized from β-cyclodextrin (β-CD), epichlorohydrin (EP) and choline chloride (CC). Indomethacin (IDM), an anionic drug, was chosen as a model drug to evaluate the drug loading capacities of CPβCDs. The formation of IDM-CPβCD complex was confirmed by 1 H NMR and DSC . Phase solubility studies and Job plots indicated that CPβCDs can solubilize IDM up to 100 times of its intrinsic solubility in a 1:1 complexation form. Mechanism studies with the help of adamantane revealed that the effective complexation is a combination of inclusion complexation, charge interaction and hydrophobic interaction. In addition, IDM-CPβCDs loaded alginate hydrogels were prepared and obtained controllable release profile in dissolution tests. The tunable structures of CPβCDs make them promising drug carriers with superior drug loading capacities and controllable drug release abilities.
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高影响因子
CARBOHYDRATE RESEARCH
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2014
·
IF 1.966
Rui-jian Jiang,Yu-lin Zhao,Yun-jian Chen,Dan Xiao,Fen Wang,Bin Han,Jian Yang,Xia-li Liao,Li-Juan Yang,Chuan-zhu Gao,Bo Yang
A novel series of artesunate-β-cyclodextrin (ATS-β-CD) conjugates, in which artesunate (ATS) was coupled covalently to one of the primary hydroxyl groups of β-cyclodextrin (β-CD) through amino bond formation, were synthesized and characterized by 1 H NMR, HRMS, 2D NMR (ROESY), X-ray diffraction (XRD), and thermogravimetric analysis (TGA). The results showed that the aqueous solubility of ATS-β-CD conjugates was 26–45 times better than that of free ATS. The cytotoxicity of the ATS-β-CD conjugates was evaluated on human colon cancer cell lines HCT116, LOVO, SW480, and HT-29, and the results indicated that ATS-2NβCD exhibited a very high cytotoxicity against HCT116, LOVO, and HT-29 with IC 50 values of 0.58, 1.62, and 5.18 μmol/L, respectively. In addition, the supposition of better cytotoxicity was further supported by the control experiment of fluorescent cyclodextrin .
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ENVIRONMENTAL TOXICOLOGY AND PHARMACOLOGY
·
2015
·
IF 2.084
Zheng Cao,Yang Fu,Xudong Sun,Qiuyue Zhang,Feibo Xu,Yanfei Li
Exposure to aluminum (Al) suppresses bone formation. Osteoblastic differentiation plays a key role in the process of bone formation. However, the effect of Al on osteoblastic differentiation is still controversial, and the mechanism remains unclear. To investigate the effect of Al on osteoblastic differentiation and whether Wnt signaling pathway was involved in it, the primary rat osteoblasts were exposed to 1/40 IC50, 1/20 IC50 and 1/10 IC50 of aluminum trichloride (AlCl 3 ) for 24 h, respectively. The activity analysis of alkaline phosphate, qRT-PCR analysis of type I collagen, alkaline phosphate, Wnt3a and Dkk-1, Western blot analysis of p-GSK3β, GSK3β and β-catenin protein and Immunofluorescence staining for β-catenin suggested that AlCl 3 inhibited osteoblastic differentiation and Wnt/β-catenin pathway. Moreover, we found exogenous Wnt3a application reversed the inhibitory effect of AlCl 3 on osteoblastic differentiation, accompanied by activating the Wnt/β-catenin pathway. Taken together, these findings suggest that AlCl 3 inhibites osteoblastic differentiation through inactivation of Wnt/β-catenin pathway in osteoblasts.
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2018
·
IF 3.909
Yu Li,Xiaoyan Hu,Jingcheng Sang,Ying Zhang,Huitu Zhang,Fuping Lu,Fufeng Liu
β-Glucosidases hydrolyze terminal, non-reducing β- d -glucosyl residues and thereby release β- d -glucose. They have applications in the production of biofuels, beverages and pharmaceuticals. In this study, a β-glucosidase derived from Aspergillus aculeatus (BGLA) was expressed, characterized, and the molecular mechanism of its acid denaturation was comprehensively probed. BGLA exhibited maximal activity at pH 5.0–6.0. Its optimal temperature was 70 °C. Its enzyme activity was enhanced by Mg 2+ , Ca 2+ and Ba 2+ , while Cu 2+ , Mn 2+ , Zn 2+ , Fe 2+ and Fe 3+ had a negative effect. BGLA showed activity on a broad range of substrates including salicin, cellobiose, arbutin, geniposide and polydatin. Finally, the acid-denaturation mechanism of BGLA was probed using molecular dynamics (MD) simulations. The results of simulation at pH 2.0 imply that the contact number, solvent accessible surface area and number of hydrogen bonds in BGLA decreased greatly. Moreover, the distance between the residues Asp280 and Glu509 that are part of the active site increased, which eventually destroyed the enzyme's catalytic activity. These MD results explain the molecular mechanism of acid denaturation of BGLA, which will greatly benefit the rational design of more acid-stable β-glucosidase variants in the future.
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CERAMICS INTERNATIONAL
·
2022
·
IF 5.532
Gangyuan Bi,Lina Mo,Sa Liu,Xiupeng Zhong,Junzhong Yang,Zhongrun Yuan,Shenggui Chen,Li Ren
Nowadays, the repair of long bone defects remains a clinical challenge mainly due to poor oxygen and nutrients delivery. In this study, β-tricalcium phosphate (β-TCP) porous ceramic scaffolds were prepared by digital light processing (DLP) and gradient sintering process. The functionalization of scaffolds was achieved by loading hyaluronic acid-dopamine (HA-DA) coating or sphingosine 1-phosphate/hyaluronic acid-dopamine (S1P/HA-DA) coating, which solved the problem of oxygen and nutrients delivery to a certain extent by promoting blood vessels growth. Cytocompatibility assay, qRT-PCR, Alkaline phosphatase (ALP) staining and quantitative analysis demonstrated that the S1P/HA-DA/TCP scaffolds significantly promoted the proliferation and osteogenic differentiation of mouse bone marrow mesenchymal stem cells (mBMSCs). Long bone defects (22 mm), rarely reported in previous studies, were constructed on the radius of rabbits. Animal experiments showed excellent early angiogenesis and bone repair in HA-DA/TCP and S1P/HA-DA/TCP groups. In particular, the S1P/HA-DA/TCP scaffolds enhanced bone regeneration and osseointegration. Overall, these functionalized scaffolds had an effective repair on long bone defects that would have great potential for clinical applications.
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TALANTA
·
2022
·
IF 6.556
Shuang Tan,Shunling Li,Congkui Tang,Xiongfei Bai,Xin Ran,Qing Qu,Lei Li,Long Yang
The conventional fluorescence analysis methods for disease identification are vulnerable to the restriction with false-positive. Here, a fluorescent switch with high efficiency and regeneration by the black phosphorus (BP) nanosheets-regulated was developed to overcome false-positive issue in the assay of β- amyloid 1−42 oligomers (A β ) process. The A β was rapidly recognized using the fluorescent emitter‐nitrogen-doped carbon nanodots (N-CDs) under the regulation of BP nanosheets , while the N-CDs alone cannot recognize A β without the introduction of BP. The fluorescence analysis methods exhibited a wide sensing range of 0.25–15.0 ng/mL and a low detection limit of 83 pg/mL for A β analysis, which was superior to the reported fluorescence analysis method. Further, BP nanosheets were recycled, demonstrating the fluorescent switch with highly efficient, stable, and regenerable. This provides a new idea for developing high-efficiency and high-precision fluorescence detection platform.
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高影响因子
Scientific Reports
·
2017
·Vol. 7
·期 1
·1-11
·
IF 4.259
Zhou Yang,Yuan Shaofei,Liu Qian,Yan Dandan,Wang Yun,Gao Li,Han Juan,Shi Haifeng
In this paper, an efficient and convenient Fe 3 O 4 /PMG/IDA-Ni 2+ nanoparticles that applied to purify and immobilize his-tagged β-glucosidase was synthesized, in which, Fe 3 O 4 /PMG (poly ( N, N’- methylenebisacrylamide-co-glycidyl methacrylate) core/shell microspheres were synthesized firstly using distillation-precipitation polymerization, then iminodiacetic acid (IDA) was used to open epoxy rings on the shell of microspheres to the combination of Ni 2+ . The gene of β-glucosidase that was from Coptotermes formosanus Shiraki was amplified, cloned into the expression vector pET28a with an N-terminal His-tag, and expressed in E.coli BL21. The nanoparticles showed the same purification efficiency as commercial nickel column which was a frequently used method in the field of purifying his-tagged proteins from crude cell lysates. The results indicated that Fe 3 O 4 /PMG/IDA-Ni 2+ nanoparticles can be considered as an excellent purification material. β-glucosidase was immobilized on the surface of Fe 3 O 4 /PMG/IDA-Ni 2+ to form Fe 3 O 4 /PMG/IDA-β-glucosidase by means of covalent bound with imidazolyl and Ni 2+ . The immobilized β-glucosidase exhibited excellent catalytic activity and stabilities compared with free β-glucosidase. In addition, immobilized β-glucosidase can be recycled for many times and retain more than 65% of the original activity. The materials display enormous potential in the aspect of purifying and immobilizing enzyme.
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