NEW JOURNAL OF CHEMISTRY
·
2018
·Vol. 42
·期 16
·13375-13381
·
IF 3.201
Haobo Han,Wenqi Chen,Jiebing Yang,Jiayuan Zhang,Quanshun Li,Yan Yang
The modification of 2-amino-6-chloropurine on polyamidoamine was performed to synthesize a derivative, AP-PAMAM, which was then employed as a carrier for p53 gene delivery to achieve anti-tumor efficacy. Using a human prostate tumor cell line PC-3 as a model, enhanced expression level of p53 gene could be detected by RT-PCR, qPCR and western blotting. Based on the successful p53 gene transfection, an obvious anti-proliferative effect could be obtained, which was identified to be associated with the simultaneous induction of cell apoptosis and cell cycle arrest at the G1 phase. The cell apoptosis was elucidated to be triggered by the activation of the mitochondria-dependent apoptotic signaling pathway. In addition, AP-PAMAM/p53 transfection could restrain the migration of tumor cells as demonstrated by wound healing and Transwell migration assays. Notably, AP-PAMAM-mediated p53 delivery exhibited a stronger inhibitory effect on both cell proliferation and migration than the PAMAM/p53 group. In summary, AP-PAMAM/p53 transfection provided a promising route to achieve the p53-based gene therapy.
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INDUSTRIAL & ENGINEERING CHEMISTRY RESEARCH
·
2019
·
IF 3.375
Honghua Zhang,Jiakuo Xu,Hongguo Tang,Zhiqiang Yang,Ruixia Liu,Suojiang Zhang
Different metal compositions based on metal-ion-modified X-type zeolite in the isobutane/2-butene alkylation reaction were investigated. Bimetallic (rare earth (RE) and Cu) supported zeolites catalysts owned better lifetime and selectivity than those modified by the single metal (La, Ce, or Cu). CuLaX performed best with a 15 h catalytic lifetime obtained after hydrogen activation when the content of copper was 0.197 mmol/g. The possible reason is that the RE X-type catalyst modified with copper ion increases the concentration of strong Brønsted acid sites, which is more favorable for the hydride transfer in the alkylation. Herein, the valence of copper ions is of importance to the alkylation performance. A mechanism was proposed wherein the existence of Cu+ inhibited the progress of the oligomerization reaction and then prolonged the lifetime of the catalyst through decreasing the desorption concentration of 2-butene in the active site.
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COLORATION TECHNOLOGY
·
2019
·Vol. 135
·期 5
·335-348
·
IF 1.241
Qiushui Zhang,Wei Xiong,Shufen Zhang,Wei Ma,Bingtao Tang
A new kind of hetero-bifunctional reactive dye containing 2-ethoxy-4-chloro- s -triazine, with better activity matching with β-hydroxyethyl sulphone sulphate at a low fixation temperature, was successfully synthesised and characterised. An ethoxy group was designed to be introduced into triazine to increase the substantivity and the reactivity of the dyes. Thereby, the fixation of mono- s -chlorotriazine/hydroxyethyl sulphone sulphate (KM-type) bifunctional reactive dyes on cotton was improved, and dyeing under mild conditions with high fixation was realised. Nineteen dyes with orange, red and blue colours were synthesised and characterised by UV-Vis and infrared spectroscopy and mass spectrometry. Synthesised dyes were dyed at 60, 75 and 90°C, respectively. Exhaustion and reactivity for all of the ethoxy-containing KM-type dyes was over 90% at the optimal temperature, and fixation was over 87%, which was higher than the ethoxy-free comparative dyes. The optimal fixation temperature of ethoxy-containing dyes was initially reduced to 75°C, and then to 60°C. Compared with comparative dyes, the wash fastness and light fastness were basically unchanged, and the wet rub fastness of the H-acid series was reduced by one grade.
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JOURNAL OF APPLIED POLYMER SCIENCE
·
2019
·Vol. 136
·期 46
·48237
·
IF 2.188
Feng Gao,Yifang Mi,Xinlei Wu,Jun Yao,Qi Qi,Zhihai Cao
Stimuli-responsive polymeric nanogels hold great potential in biological applications. In this work, thermoresponsive polymeric nanogels were conveniently prepared through inverse miniemulsion polymerization of two monomers with a good biocompatibility, N -vinylcaprolactam and 2-methoxyethyl acrylate. A macromolecular crosslinker, poly(ethylene glycol) dimethacrylate (PEGDMA), was used to achieve a better thermoresponsiveness, compared with low-molecular-weight crosslinkers. The prepared poly( N -vinylcaprolactam- co -2-methoxyethyl acrylate) (poly(NVCL- co -MEA)) nanogels could be well redispersed in aqueous systems, displaying a reversible thermoresponsive transition behavior. The influences of the synthesis parameters including the emulsifier content, PEGDMA content, and monomer composition on the particle properties of poly(NVCL- co -MEA) nanogels both in inverse emulsions and in aqueous dispersions were systematically investigated. Furthermore, the impacts of the monomer composition and PEGDMA content on the thermoresponsiveness of poly(NVCL- co -MEA) nanogels were also studied. Promisingly, the introduction of MEA monomeric units to the copolymer chains only slightly reduced the thermoresponsiveness of poly(NVCL- co -MEA) nanogels. This feature allows to improve the biocompatibility of polymeric nanogels by using MEA as the comonomer without need to compromise the thermoresponsiveness of nanogels. © 2019 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2019 , 136 , 48237.
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JOURNAL OF HAZARDOUS MATERIALS
·
2019
·
IF 7.65
Jiafei Su,Cuilian Cheng,Yaping Guo,He Xu,Qinfei Ke
Cryptomelane-type octahedral molecular sieve (OMS-2) catalysts are currently attracting tremendous attention due to their low-cost and remarkable thermo-catalytic activity. However, it is still difficult for OMS-2 catalysts to completely degrade formaldehyde at relatively low or even ambient temperature. In this work, OMS-2 catalysts with different ratios of length to diameter were prepared and the OMS-2-s with the minim ratio of length to diameter (1–3) exhibited the best catalytic performance than the other samples. Then, the optimized OMS-2-s nanorods were loaded on the SiO 2 nanofibers via a simultaneous electrospining-spray strategy. The evaluation for the dynamic catalytic activities of the samples showed that, the T 50 (HCHO conversion reached to 50%) for the OMS-2/SiO 2 nanofibrous membranes was decreased by 24 °C than the OMS-2-s nanorods. Furthermore, in the static experiment of HCHO decomposition, the composite membrane could achieve a catalytic efficiency of 52.3% at 25 °C, much higher than that of the OMS-2-s nanorods (45.9%). This work offers a new strategy to improve the catalytic efficiency of OMS-2 by controlling the morphology and loading of OMS-2 nanorods, and also designs a kind of advanced nano OMS-2-based nanofibrous membranes with hierarchical nanostructures for the highly efficient formaldehyde elimination during the practical application.
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高影响因子
INTERNATIONAL JOURNAL OF ANTIMICROBIAL AGENTS
·
2017
·
IF 4.307
Hong-Yan Luo,Ma-Feng Liu,Ming-Shu Wang,Xin-Xin Zhao,Ren-Yong Jia,Shun Chen,Kun-Feng Sun,Qiao Yang,Ying Wu,Xiao-Yue Chen,Francis Biville,Yuan-Feng Zou,Bo Jing,An-Chun Cheng,De-Kang Zhu
The Gram-negative bacterium Riemerella anatipestifer CH-2 is resistant to lincosamides, having a lincomycin (LCM) minimum inhibitory concentration (MIC) of 128 µg/mL. The G148_1775 gene of R. anatipestifer CH-2, designated lnu (H), encodes a 260-amino acid protein with ≤41% identity to other reported lincosamide nucleotidylyltransferases. Escherichia coli Rosetta TM (DE3) containing the pBAD24- lnu (H) plasmid showed four- and two-fold increases in the MICs of LCM and clindamycin (CLI), respectively. A kinetic assay of the purified Lnu(H) enzyme for LCM and CLI showed that the protein could inactive lincosamides. Mass spectrometry analysis demonstrated that the Lnu(H) enzyme catalysed adenylylation of lincosamides. In addition, an lnu (H) gene deletion strain exhibited 512- and 32-fold decreases in LCM and CLI MICs, respectively. The wild-type level of lincosamide resistance could be restored by complementation with a shuttle plasmid carrying the lnu (H) gene. The transformant R. anatipestifer ATCC 11845 [ lnu (H)] acquired by natural transformation also exhibited high-level lincosamide resistance. Moreover, among 175 R. anatipestifer field isolates, 56 (32.0%) were positive for the lnu (H) gene by PCR. In conclusion, Lnu(H) is a novel lincosamide nucleotidylyltransferase that inactivates LCM and CLI by nucleotidylylation, thus conferring high-level lincosamide resistance to R. anatipestifer CH-2.
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CORROSION SCIENCE
·
2017
·
IF 5.245
Mingjun Cui,Siming Ren,Songlv Qin,Qunji Xue,Haichao Zhao,Liping Wang
Hexagonal boron nitride (h-BN), a structural analogue of graphene, is a better alternative for anticorrosive coatings because of its electrical insulation properties. In this work, few-layer h-BN nanosheets are obtained by the exfoliation of stacked h-BN powders with poly(2-butyl aniline) (PBA) and incorporated into epoxy coatings for protecting metallic substrate against corrosion. Results show that as prepared composite coatings exhibit high impedance modulus and low water absorption, suggesting their superior performance of corrosion protection owing to the “labyrinth effect” of h-BN and passivation effect of PBA on the metal substrates.
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JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2017
·
IF 3.154
Qianzhu Zhao,Yueyu Zou,Caihuan Huang,Ping Lan,Jie Zheng,Shiyi Ou
Adducts of 5-hydroxymethylfurfural (HMF)-amino acids are formed during food processing and digestion; the elimination capacity of in vitro intestinal digests of biscuits, instant noodles, and potato crisps for HMF is 652, 727, and 540 μg/g, respectively. However, the safety of these adducts is unknown. In this study, an HMF–cysteine adduct named 1-dicysteinethioacetal-5-hydroxymehtylfurfural (DCH), which was found to be produced in the gastrointestinal tract after HMF intake, was prepared to test its effect toward Caco-2 cells. Compared with HMF, the adduct displayed lower cytotoxicity against Caco-2 cells with an IC50 value of 31.26 mM versus 14.95 mM (HMF). The DCH did not induce cell apoptosis, whereas HMF significantly increased the apoptosis rate after incubation at concentrations of 16, 32, and 48 mM for 72 h. DCH showed an absorption rate considerably lower than that of HMF by Caco-2 cells. Lower absorption of DCH may result in lower toxicity compared with HMF against Caco-2 cells. Intracellular transformation of DCH has been observed.
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MICROCHEMICAL JOURNAL
·
2024
·
IF 4.9
Xu Yang,Zixuan Liu,Yong Kong,Zheng-Zhi Yin,Guojun Zheng,Hongyu Zhang
An anti-fouling surface molecularly imprinted (SMI) ratiometric electrochemical biosensor was fabricated for the detection of severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) spike protein (S protein). Polypyrrole (PPy) was electrodeposited on the electrode surface by using ferrous phthalocyanine (PcFe) and sodium alginate (SA) as the dopants, which acted as the molecularly imprinted polymers (MIPs). The SMI electrochemical biosensor could be used for the detection of SARS-CoV-2 S protein based on the ratiometric signals of the internal reference (PcFe) and the external probe ([Fe(CN) 6 ] 3−/4− ). Under optimized conditions, the as-fabricated SMI ratiometric biosensor showed a wide linear range of 100 fg mL −1 ∼ 100 ng mL −1 with a low limit of detection (LOD) of 30.1 fg mL −1 . Nonspecific adsorption of interfering substances onto the SMI biosensor surface could be significantly reduced due to the outstanding anti-fouling ability of SA, as revealed by fluorescence microscopy. Finally, the reliability of the SMI ratiometric electrochemical biosensor was demonstrated by the detection of SARS-CoV-2 S protein in clinical serum samples. The findings of this work open a new avenue in clinical diagnosis of COVID-19.
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SYNLETT
·
2011
·Vol. 2012
·期 01
·145-149
·
IF 2.447
Wenpeng Mai,Jinwei Yuan,Zhicheng Li,Gangchun Sun,Lingbo Qu
All articles of this category A novel direct arylation of pyridine N -oxideswith arylboronic acids through C-H functionalization hasbeen developed. This new reaction is performed at room temperatureusing catalytic silver(I) nitrate in the presence of potassium persulfateand give 2-pyridyl arylation derivatives of pyridine N -oxides.
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JOURNAL OF ALLOYS AND COMPOUNDS
·
2024
·
IF 5.8
Changming Zhang,Zhihao Liu,Yongjin Zou,Fen Xu,Lixian Sun,Zhicong Hu,Sensen Yu,Cuili Xiang
Spinel-type materials are attracting great attention as potential electrocatalysts for the hydrogen- and oxygen-evolution reactions. However, their inherent catalytic activities are not on par with those of precious-metal catalysts, and their poor stabilities continue to constrain their rapid development. In this study, we fabricated Zr-CuCo 2 S 4 nanowires and Co(OH) 2 nanosheet–nanowires using electrochemical methods, resulting in lattice defects, sulfur vacancies, and more exposed active sites that catalyze the hydrogen- and oxygen-evolution reactions. DFT calculations revealed that sulfur vacancies enhance intrinsic activity, while Zr doping reduces the energy barrier for H*–OH* adsorption, promotes the formation of additional sulfur vacancies, and modulates active-site Cu/Co valence states, which greatly enhances water-dissociation and H*-adsorption kinetics. Consequently, Zr-CuCo 2 S 4 /Co(OH) 2 requires low overpotentials of 88 and 208 mV, respectively, to support highly stable alkaline hydrogen- and oxygen-evolution reactions at 10 mA cm −2 . This study provides a rational strategy for designing low-cost spinels for green and clean energy.
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MOLECULAR CELL
·
2024
·
IF 14.5
Wei Luo,Yunyuan Xu,Jie Cao,Xiaoyu Guo,Jingdan Han,Yuanyuan Zhang,Yuda Niu,Meiling Zhang,Yi Wang,Guohua Liang,Qian Qian,Song Ge,Kang Chong
While it is known that temperature sensors trigger calcium (Ca 2+ ) signaling to confer cold tolerance in cells, less is known about sensors that couple with other secondary messengers. Here, we identify a cold sensor complex of CHILLING-TOLERANCE DIVERGENCE 6 (COLD6) and osmotin-like 1 (OSM1), which triggers 2′,3′-cyclic adenosine monophosphate (2′,3′-cAMP) production to enhance cold tolerance in rice. COLD6, which is encoded by a major quantitative trait locus (QTL) gene, interacts with the rice G protein α subunit (RGA1) at the plasma membrane under normal conditions. Upon exposure to chilling, cold-induced OSM1 binds to COLD6, kicking out RGA1 from interaction. This triggers an elevation of 2′,3′-cAMP levels for enhancing chilling tolerance. Genetic data show that COLD6 negatively regulates cold tolerance and functionally depends on OSM1 in chilling stress. COLD6 alleles were selected during rice domestication. Knockout and natural variation of COLD6 in hybrid rice enhanced chilling tolerance, hinting design potential for breeding. This highlighted a module triggering 2′,3′-cAMP to improve chilling tolerance in crops.
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