Cell Communication and Signaling
·
2026
·
IF 11.6
Deng Weiqian,Yang Xiaoting,Wu Hao,Tang Haojun,Wang Xiaodan,Tu Shuangxi,Yin Kai,Zhu Xiao
Metabolic-associated fatty liver disease (MAFLD), the most prevalent chronic hepatic disorder globally, is pathologically characterized by excessive intrahepatic lipid deposition, oxidative stress, and chronic low-grade inflammation. Apolipoprotein A-I binding protein (AIBP), a critical modulator of lipid metabolism, cellular signaling pathways, inflammatory responses, and metabolic homeostasis, has not been thoroughly investigated in the context of MAFLD pathogenesis. In this study, we identified a significant downregulation of AIBP expression in both high-fat diet (HFD)-induced MAFLD murine models and palmitic acid (PA)-treated HepG2 cells. Systemic AIBP deficiency exacerbated metabolic dysregulation and induced profound perturbations in hepatic architecture and function, culminating in aggravated liver injury. This was evidenced by enhanced steatosis, elevated pro-inflammatory cytokine production, and increased serum aspartate aminotransferase (AST) and alanine aminotransferase (ALT) levels. Transcriptomic profiling and molecular characterization of AIBP-deficient HepG2 cells corroborated these pathological alterations. Mechanistic investigations revealed that AIBP silencing potentiates hepatic damage through integrin β3 (ITGβ3)-mediated hyperactivation of the phosphoinositide 3-kinase (PI3K)/protein kinase B (AKT) signaling cascade. Notably, hepatocyte-specific AIBP overexpression effectively ameliorated these metabolic and inflammatory aberrations. Collectively, our findings establish AIBP as a pivotal hepatoprotective regulator in MAFLD pathogenesis and propose a novel therapeutic paradigm for MAFLD and associated metabolic disorders through targeted AIBP modulation. This study provides compelling evidence for the development of AIBP-centered therapeutic strategies to mitigate MAFLD progression.
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Drug Delivery and Translational Research
·
2026
·
IF 6.9
Zhang Rui Xue,Lu Weijia,Yan Xu,Deng Xudong,Wan Runrun,Song Zhejun,Liang Jiayan,Wang Xue-Ting,Cao Tingting,Chen Yan,Tam Paul K. H.
Precise dosing is critical for effective therapeutics, yet extensive enterohepatic metabolism poses a major barrier to many orally administered small-molecule drugs. Budesonide, a potent corticosteroid for treating enterohepatic inflammation, undergoes significant first-pass metabolism by cytochrome P450 3A abundantly expressed in the liver and intestine, resulting in variable efficacy and unpredictable toxicity. Rectal drug delivery offers an alternative route that can reduce first-pass metabolism. This work aimed to alter enterohepatic metabolism and clearance of budesonide using a rectal nanoparticles-hydrogel system for localized drug delivery. Budesonide was encapsulated into nanostructured lipid carrier (BD-LNP), which were then entrapped in a dynamic boronate ester crosslinked hydrogel (BD-LNP@Hydrogel). The hydrogel network exhibited thermal and mechanical stability, maintaining a drug depot post-injection, while BD-LNP was released in a controlled manner upon hydrogel degradation in simulated colonic fluid. BD-LNP@Hydrogel achieved a permeability coefficient > 1 × 10 –6 cm·s −1 across human intestinal epithelial monolayers, indicating completed absorption of budesonide. Compared to BD-LNP alone, BD-LNP@Hydrogel provided 4.3-fold higher nanoparticle exposure in the colonic lumen, enhancing intestinal uptake into deeper mucosal layer (e.g., lamina propria) over time. Pharmacokinetic analysis in mice revealed that rectal BD-LNP@Hydrogel markedly reduced formation of the budesonide metabolite 16α-hydroxyprednisolone compared to the oral route. Physiologically-based pharmacokinetic modeling confirmed preferential pharmacokinetics for rectal BD-LNP@Hydrogel over oral delivery, including up to 7.3-fold higher absorption rate constant, superior intestinal transport, and significantly reduced enterohepatic clearance of 16α-hydroxyprednisolone. These findings highlight the potential of nanoparticles-hydrogel systems for rectal delivery of drugs with extensive metabolism and provide mechanistic insight into achieving therapeutic precision via rectal delivery. Graphical
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ACS Applied Nano Materials
·
2026
·Vol. 9
·期 31
·14854-14871
·
IF 5.8
Zhu Lijun,Xiong Jiabao,Du Zhong,Ma Kaikai,Chu Chenyang,Zhang Chi,Huang Siyu,Gao Yuxiang,Zhang Linxue,Wu Qinfen,Dong Biao,Alifu Nuernisha
Hollow manganese-dioxide-based nanoplatforms, which combine
hollow
structures with the intrinsic theranostic properties of Mn 2+ , have shown great potential for precise tumor diagnosis and treatment.
However, single imaging or therapeutic modalities are still insufficient
for high requirements of precise cancer theranostics. Herein, a hollow
manganese-based theranostic nanoplatform, MnO 2 –IR-820-Q/DOX@TMTP1
nanocomposite (Mn-NCs), was designed and synthesized. With the assistance
of the Mn-NCs, near-infrared-II (NIR-II, 900–1700 nm) fluorescence
imaging with high spatial resolution and low autofluorescence was
combined with magnetic resonance imaging (MRI) for highly efficient
and precise multimodal imaging of cervical tumors. The organic cyanine
dye IR-820 and PbS@CdS quantum dots (QDs) were integrated to form
a hybrid nanocomposite (IR-820-Q) with strong NIR-II fluorescence
emission, which was subsequently assembled onto a MnO 2 nanocarrier
to enable MRI and protect IR-820-Q from degradation. The hollow MnO 2 nanoshells served as a nanoscale structural framework for
IR-820-Q/DOX loading, tumor microenvironment-responsive degradation,
and Mn 2+ -mediated imaging/therapeutic functions. The cervical
tumor-targeting peptide TMTP1 was introduced to improve the tumor-targeting
potential of the nanocomposite, enabling NIR-II fluorescence visualization
of primary tumors and lymph node metastatic lesions. Therefore, Mn-NCs
are well suited for tumor-targeted imaging-guided intervention of
cervical cancer and lymphatic metastasis. By constructing the theranostic
nanoplatform, the photothermal therapy (PTT) as well as photodynamic
therapy (PDT) effect from the IR-820 was enhanced, and with the hydroxyl
radicals (via Fenton-like reaction) of Mn 2+ in the tumor
microenvironment, effective chemodynamic therapy (CDT) was also achieved.
The loading of doxorubicin (DOX) further endowed the nanoplatform
with a chemotherapeutic capability. Thus, the developed theranostic
nanoplatform showed strong NIR-II fluorescence/MRI-guided synergistic
PTT/PDT/CDT and chemotherapy, which significantly improved the therapeutic
outcomes. This study demonstrates a promising strategy for precise
cervical cancer diagnosis and treatment, highlighting the potential
of organic–inorganic hybrid nanomaterials for clinical translation.
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POLYMER BULLETIN
·
2026
·Vol. 83
·期 10
·561
·
IF 4.2
Sebbar Khaoula,Ibnsouda Saad Koraichi,Damiri Fouad,Fatimi Ahmed,Moudani Nada Al,Aarab Lotfi,Ilhan Elif,Altan Eray,Gunduz Oguzhan,Houmada Amine Ait,Lyamlouli Karim,Elabed Soumya
Argan nutshell lignin (ANS-LIG) is a sustainable biomaterial recognized for its notable antioxidant and antimicrobial properties. This study investigates its application in 3D-printed (3DP) wound dressings, infused with Moroccan essential oils (EOs), specifically Mentha pulegium (MPEO) and Rosmarinus officinalis (ROEO), to reinforce its antibacterial efficacy. ANS-LIG and EOs were integrated into Eudragit RS PO (EudRP) matrices and printed at room temperature to protect the bioactive properties. Analyses using SEM, ATR-FTIR, and XRD revealed the formation of highly porous, lightweight meshes, with ANS-LIG crystallographic planes becoming partially amorphous when combined with EUD and EOs. Thermal analysis demonstrated improved stability for LIG/EUD + EOs compared to LIG/EUD alone. Studies on hydrophilicity and swelling indicated substantial water absorption, while DPPH assays confirmed strong antioxidant activity within 15 min. Antibacterial tests against gram-positive and gram-negative bacterial strains demonstrated significant activity; in particular, Staphylococcus aureus viability was reduced by 93% after 24 h using the LIG/EUD+MPEO 3D printed patch. Lastly, for the in vitro biocompatibility assay, mouse dermal fibroblasts (MTT assay) were utilized, confirming promising cytocompatibility, with ROEO-loaded meshes exhibiting the highest cell viability of 220%, while MPEO demonstrated more efficient antimicrobial results across all tested strains. Ultimately, the obtained 3D-printed scaffolds efficiently integrated sustainable biopolymers mixed with potent antimicrobials and antioxidant agents, alongside impressive biocompatibility, indicating their potential as advanced wound dressings. This study underscores the advantages of incorporating ANS-LIG and EOs into 3D-printed formulations for future biomedical applications. Graphical abstract
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Antioxidants
·
2026
·Vol. 15
·期 7
·866
·
IF 8.2
Yiming Luo,Chengbin Feng,Mengze Liu,Pengfei Zhao,Danfei Huang
Selenium nanoparticles (SeNPs), which are nanoscale particles of elemental selenium (Se 0 ), combine the biological activity of selenium with the distinctive properties inherent in nanomaterials, thereby making SeNPs a more promising candidate for advancing selenium-based resources. In this work, several strains of Bacillus with sodium selenite-reducing ability were isolated from selenium-rich soils. Bacillus subtilis ESNS-2 with high selenium tolerance was selected as the target strain. Under culture conditions supplemented with 5 mmol/L sodium selenite, this strain exhibited a reduction efficiency of 75.4 ± 0.6% over 24 h. The produced bioSeNPs were purified, decorated using polysaccharides from the seeds of Plantago asiatica L. (PLP), and subsequently systematically characterized using various means. The results revealed that the prepared PLP-bioSeNPs were regularly spherical elemental selenium particles, with an average particle size of 96.9 ± 1.1 nm, a PDI of 0.108 ± 0.003, and a zeta potential of −19.7 ± 0.4 mV. Characterization confirmed that they exhibited excellent dispersibility and stability. In vitro antioxidant assays, both bioSeNPs and the PLP-bioSeNP complex demonstrated pronounced dose-dependent scavenging activity against DPPH• and ABTS + • radicals. In a D-galactose-induced aging mouse model, both bioSeNPs and PLP-bioSeNPs alleviated D-galactose-induced hepatic and cerebral damage, as well as associated behavioral deficits, through the modulation of oxidative stress balance and suppression of inflammation. This study successfully accomplished the efficient production of SeNPs utilizing Bacillus subtilis ESNS-2. The modification of PLP provided innovative strategies for the development of macromolecular drugs, with a specific emphasis on enhancing stability.
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ANALYTICAL AND BIOANALYTICAL CHEMISTRY
·
2026
·
IF 4.1
Yang Moying,Wen Wu,Yang Hongxian,Di Bin,Chen Jie,Su Mengxiang
Mescaline and lysergic acid diethylamide (LSD) are strong psychedelics that significantly compromise social safety and public health. Satisfying the rapid detection requirements for public security drug enforcement is challenging because of the high costs and long durations associated with gas chromatography–mass spectrometry (GC–MS) and liquid chromatography–mass spectrometry (LC–MS). To address these challenges, this study established an immunochromatographic method for the simultaneous detection of mescaline and LSD in hair based on time-resolved fluorescent microspheres. By optimizing key parameters such as the type and pH of the activation buffer, the pH of the labeling buffer, the concentrations of the detection lines (T 1 /T 2 ) and the control line (C line), and the antibody labeling amount, under optimal conditions, the detection limits of this method for mescaline and LSD were 16.5 pg/mg and 12.5 pg/mg, respectively. The recovery rate ranged from 94.98 to 109.83%, and the coefficient of variation ranged from 2.24 to 8.48%, indicating excellent sensitivity, specificity, and accuracy. The detection results of real samples were essentially consistent with those of LC–MS/MS. Consequently, this method allows preliminary testing of hair samples in forensic labs, providing an efficient, reliable prescreening tool before LC–MS/MS confirmation. Graphical abstract
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Pharmaceutics
·
2026
·Vol. 18
·期 9
·1090
·
IF 6.9
Chao Fang,Lixin Xie,Daihan Xie,Jingting Yin,Shiji Zhang,Yiling Gan,Xiaodong Hou,Fanlei Kong,Yu Huo,Xiuli Liu
Introduction: Acute lung injury (ALI) is characterized by severe inflammation and oxidative stress. However, effective pharmacological interventions for ALI remain limited. Carnosine, an endogenous dipeptide known for its redox-regulating and immunomodulatory activities, has demonstrated promising protective effects. In the present study, inhalable carnosine-loaded liposomes (Cn-Ls) were developed to enhance pulmonary delivery and achieve localized treatment in a lipopolysaccharide (LPS)-induced ALI model in mice.Methods: Cn-Ls were prepared and systematically evaluated for their morphology, stability, drug-loading capacity, and release kinetics. In vitro assays were performed to evaluate their cytocompatibility, antioxidant activity, and effects on LPS-induced reactive oxygen species (ROS) generation. In an ALI model, inhaled Cn-Ls were administered to assess pulmonary retention and therapeutic efficacy, including lung inflammation, oxidative stress, circulating levels of C-reactive protein (CRP), tumor necrosis factor (TNF)-α, interleukin (IL)-6, lung architecture, and respiratory function.Results: Encapsulation of carnosine within liposomes markedly prolonged its pulmonary retention (t1/2= 1.7 h vs. 1.0 h for free carnosine), providing a more sustained lung-retentive delivery profile for up to 12 h. In vitro assays showed that Cn-Ls had excellent cytocompatibility, reduced cell death, exhibited potent antioxidant activity, and effectively suppressed LPS-induced ROS generation. In an ALI model, inhaled Cn-Ls markedly mitigated lung inflammation and oxidative stress, reduced circulating levels of CRP, TNF-α, and IL-6, preserved lung architecture, and improved respiratory function. Compared with free carnosine, Cn-Ls exhibited enhanced pulmonary retention and superior therapeutic efficacy.Conclusions: These results identified inhalable Cn-Ls as a potential nanotherapeutic approach for targeted ALI management and provided a foundation for further translational development. However, additional investigations are required to assess the long-term safety of Cn-Ls, optimize formulation stability and scalability, and further elucidate the underlying therapeutic mechanisms before clinical translation.
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ANALYTICAL CHEMISTRY
·
2026
·
IF 7.3
He Yijing,Yang Yue,Wang Huixi,Zhang Fan,Cai Wentao,Yu Haixiang,Di Bin
Dextromethorphan (DXM) is a widely used cough suppressant,
and
its escalating abuse is a pressing global health concern that necessitates
rapid screening tools. However, rapid methods for detecting DXM in
complex matrices remain highly limited. Here, we report the first
isolation and engineering of DNA aptamers specifically targeting DXM.
Using library-immobilized SELEX, we identified high-affinity aptamers
( K D ∼ 1–2 μM) with
exceptional specificity against various interferents. The lead candidate
was rationally truncated into a minimal 27-nucleotide core (DM1–27)
that retains full function. Systematic mutagenesis and molecular modeling
further delineated the structure–activity relationship governing
target recognition. Utilizing this engineered aptamer, we developed
two distinct fluorescent platforms: a label-free thioflavin T displacement
assay and a fluorophore-quencher labeled hybrid beacon. Both sensors
enabled robust DXM quantification within 10 min in human saliva and
e-cigarette juice (EJ), achieving limits of detection at low ng/mL-level.
Notably, the hybrid beacon significantly improved matrix tolerance
by bypassing short-wavelength background interference. This work provides
a versatile molecular recognition tool and establishes practical,
field-deployable sensing strategies for DXM monitoring.
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MICROCHIMICA ACTA
·
2026
·Vol. 193
·期 9
·649
·
IF 5.4
Cai Chuangui,Lin Zhen,Yao Wensong,Lian Shan,Huang Kaiyuan,Deng Haohua,Lin Xinhua,Chen Wei
Arginine surface-engineered 6-aza-2-thiothymine gold nanoclusters (Arg/ATT-AuNCs) via host-guest assembly were synthesized which exhibit exceptional catalytic activity and intense photoluminescence. Arg/ATT-AuNCs enhanced the CL intensity from NaIO 4 -H 2 O 2 system by 1200-fold. The CL mechanistic studies revealed that Arg/ATT-AuNCs not only accelerated decay of H 2 O 2 to form reactive oxygen species, but also acted as efficient energy acceptors of singlet oxygen ( 1 O 2 ) via a CL resonance energy transfer (CRET) pathway. It was observed that doxorubicin (DOX) quenches the CL signal through an inner filter effect that disrupts the CRET process. Based on this, a straightforward, rapid, and highly sensitive biosensor has been developed for the detection of DOX in human serum samples, achieving a low detection limit of 0.029 µM. The biosensor was applied to DOX determination in human serum samples with recoveries ranging from 91.1 to 106.2%. This study not only provides a new method for the detection of DOX, but also broadens the application scope of AuNCs in the field of CL. Graphical
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Nature Communications
·
2026
·
IF 18.1
Huang Kai,Liu Hui-Zhi,Liu Yuan,Lyu Jia-Yan,Zhang Qing-Yi,Feng Zi-Yuan,Wang Zi-Lin,Dong Hui,Nie Rong,Zhang Yi-Wen,Zhang Yue-Qi,Zeng Yi,Xie Hui-Qi,Zhou Zhou,Shen Bin
Osteoarthritis (OA) remains difficult to treat because drug transport within cartilage is restricted by its dense extracellular matrix and rapid joint clearance. Here we show that hybrid exosomal nanomotors (CAP-Mn/140@hyExos) convert pathological reactive oxygen species (ROS) into transport-driving cues to overcome diffusion-limited transport within cartilage. In the oxidative OA microenvironment, manganese dioxide (MnO₂) catalyzes H₂O₂ decomposition, driving ROS-responsive transport, while a cartilage-targeting peptide enhances matrix interaction, together promoting cartilage accumulation. This strategy achieves deeper penetration (~140 μm), greater cellular uptake (~2.5-fold), and prolonged intra-articular retention (~30% remaining at two weeks). Enhanced transport enables efficient intracellular delivery of miR-140 and MnO₂ nanoparticles, attenuating oxidative stress and promoting cartilage regeneration. In a rat OA model, this therapeutic strategy improves gait performance, restores subchondral bone microarchitecture, and suppresses OA progression. These findings establish pathological microenvironments as endogenous drivers of intratissue drug transport, providing a transport-regulated strategy for drug delivery in transport-restricted tissues.
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JOURNAL OF DISPERSION SCIENCE AND TECHNOLOGY
·
2026
·
IF 2.2
Yaohui Ni,Shicheng Tang,Haiyang Wu,Yufei Zhang,Saijiao Tang,Lexin Chen,Tianjian Lu,Defeng Xu,Hang Hu
Dimethylcurcumin (DMC) is a derivative of curcumin. Studies have shown that DMC can enhance the degradation of androgen receptors (AR), thereby inhibiting AR-related diseases. However, the extremely poor water solubility and fast metabolism in vivo of DMC greatly limit its clinical applications. In the present study, poly(ethylene glycol)-modified liposomes loaded with DMC (DMC@PEG-Liposomes) were prepared by an improved membrane hydration method. The prescription of the liposomes was optimized to obtain DMC@PEG-Liposomes with a particle size between 110 and 150 nm, moderate encapsulation efficiency and good freeze-drying stability. In vitro release studies showed that DMC@PEG-Liposomes displayed Fickian release at pH 7.4 and non-Fickian release at pH 5.0. Compared with free DMC, DMC@PEG-Liposomes were more toxic to 22Rv1 and HepG2 cells in vitro. DMC@PEG-Liposomes also exhibited enhanced AUC0-t as compared to free DMC in in vivo pharmacokinetic studies. These results suggest that DMC@PEG-Liposomes are a promising DMC formulation for anticancer DMC delivery.
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ACS Applied Bio Materials
·
2025
·
IF 4.7
Yang Wang,Dianpeng Li,Min He,Gang Wang,Shulin Zhao
The treatment of non-small cell lung cancer (NSCLC) primarily relies on chemotherapy following surgical intervention. However, many late-stage NSCLC patients exhibit drug resistance and experience significant side effects from current chemotherapy agents, ultimately leading to disease progression. Consequently, the development of strategies to enhance efficacy while minimizing side effects through multimodal synergistic treatment technologies remains challenging. Here, we prepared a stable multifunctional nanocomplex, PLGA@DTX@Fe3+-TA/GOX (PDFTG), which utilizes poly(lactic acid-co-glycolic acid) (PLGA) nanoparticles to encapsulate docetaxel (DTX) as the core component, while a Fe3+-tannic acid (TA) nanonetwork serves as the outer coating. Additionally, interactions between polyphenols and proteins facilitate the loading of glucose oxidase (GOX). After intravenous injection, PDFTG selectively accumulates in the tumor region, subsequently releasing Fe3+, TA, DTX, and GOX, thereby remodeling the tumor microenvironment. Following this process, Fe3+ is reduced to Fe2+ by TA, leading to mitochondrial damage in cancer cells and resulting in an increase in O2 content. This increase promotes the GOX-catalyzed glucose oxidation reaction that generates gluconic acid and H2O2, initiating starvation therapy. The gluconic acid enhances the acidity of the microenvironment, further promoting the release of PDFTG nanoparticles and enhancing the accumulation of H2O2 within the cell. The accumulated H2O2 reacts with Fe2+ in a strongly acidic environment, leading to the of hydroxyl free radicals that initiate chemical dynamic therapy (CDT). Moreover, mitochondrial damage results in a reduction of adenosine triphosphate levels, which decreases the expression of P-glycoprotein, thereby reversing DTX resistance and producing potent multimodal synergistic antitumor effects. Multimode treatment strategies for reshaping the tumor microenvironment offer an approach with significant clinical application potential for the efficient treatment of NSCLC.
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