Materials Science & Engineering C-Materials for Biological Applications
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2018
·
IF 5.08
Fengxuan Han,Yuanbin Hu,Jiaying Li,Jiawei Gong,Qianping Guo,Caihong Zhu,Xuesong Zhu,Huilin Yang,Bin Li
The development of an ideal scaffold material is critical for the repair of bone defects. Being an important precursor of the mineralized matrix of bone tissue, octacalcium phosphate (OCP) has been considered a promising bone substitute. However, its application is largely limited due to the thermodynamical instability and poor processability of it. In this study, OCP was prepared by co-precipitation in the presence of small amount of silk fibroin (SF), which regulated the crystallization of OCP and led to the formation of SF-OCP complex. The diameters of OCP crystals in OCP, 0.1SF-OCP, 0.3SF-OCP and 1SF-OCP complexes were 489.0 ± 399.1 nm, 102.2 ± 50.7 nm, 94.7 ± 48.4 nm and 223.7 ± 167.6 nm, respectively. However, the shape of OCP crystals did not apparently change by the presence of SF. Further, porous SF/OCP composite scaffolds with pore size of 111.9 ± 33.1 μm were prepared, in which small crystals of SF-OCP complex were embedded in a SF matrix. MC3T3-E1 cells could attach and proliferate well on both the rugged surfaces and the pores of SF/OCP scaffolds, indicating their decent biocompatibility. Further, SF/OCP scaffolds markedly promoted bone regeneration in a rat calvarial critical-sized defect model. Both micro-CT and H&E characterizations showed that bone formation not only occurred around the scaffolds, but also penetrated into their center. Therefore, such SF/OCP composite scaffolds may have potential applications in bone tissue engineering.
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高影响因子
CATALYSIS LETTERS
·
2018
·Vol. 148
·期 7
·1827-1833
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IF 2.911
Xu Weina,Wang Zheyu,Chen Gong,Fu Zhongwang,Jiang Guoqiang,Chen Jian,Wu Jianzhong,Liu Zheng
Abstract We report a facile method for encapsulation of carbonic anhydrase (CA) with magnetic nanogel (MNP-CA nanogel), in which amino and vinyl groups are first grafted onto the surface of magnetic Fe 3 O 4 nanoparticles (MNP) using 3-aminopropyltriethoxysilane (APTES) and N -acryloylsuccinimide (NAS), followed by CA attachment with glutaraldehyde and in situ polymerization with acrylamide. The MNP-CA nanogel shows much improved thermostability at elevated temperatures, e.g. staying unchanged at 60 °C for 80 min, owing to multiple linkage with the hydrophilic polymer network coated at the MNP surface. Addition of the MNP-CA nanogel into a methyldiethanolamine (MDEA) solution at an ultralow mass ratio (1:10 4 ) is able to enhance the CO 2 absorption rate at 60 °C by 1.45 fold. The CA-enhanced mass transport of the absorbate has been demonstrated in a wetted-wall column, in which the MNP-CA nanogel increases the total mass transfer coefficient in the gas phase (K G ) by 4.61 fold compared with that determined in the MDEA solution. The greatly increased absorption performance and much enhanced stability make MNP-CA nanogel appealing for industrial applications. Graphical Abstract
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2018
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IF 3.909
Minghao Fan,Xun Sun,Yilin Qian,Ying Xu,Dongfeng Wang,Yanping Cao
Total tea polysaccharides (TTPS) were extracted from two kinds of pruning leaves of tea plant and fractionated into neutral tea polysaccharides (TPSI) and acidic tea polysaccharides (TPSII) by anion exchange resin D315. Some physicochemical properties, including structure, monosaccharide composition, and molecular weight distribution, as well as the 4 in vitro antioxidant activities and inhibitory effects on α-glucosidase of above polysaccharides before and after removing metal ions were investigated. By comparing TTPS and TPSII, we found that they exhibited different antioxidant activities and inhibitory actions against α-glucosidase after their metal ions were removed. However, the in vitro antioxidant activities and inhibitory effects on α-glucosidase of TPSI were substantially improved. The study can be a certain reference for tea and soil selection. At the same time, we suggested that pruning leaves of tea plant could be treated as a potential resource for the development of polysaccharide antioxidants and hypoglycemic products.
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Frontiers in Immunology
·
2018
·
IF 5.511
Chen Lu,You Qian,Hu Liang,Gao Jian,Meng Qianqian,Liu Wentao,Wu Xuefeng,Xu Qiang
Management of inflammatory bowel disease (IBD) is a real clinical challenge. Despite intense investigation, the mechanisms of IBD remain substantially unidentified. Some inflammatory conditions, such as matrix metalloproteinases (MMPs) and the nuclear factor-κB (NF-κB) and NOD-like receptor protein 3 (NLRP3) inflammasome signaling pathways, are reported to contribute to the development and maintenance of IBD. Regulation of their common upstream signaling, that is, reactive oxygen species (ROS), may be important to control the progression of IBD. In the present study, we found that procyanidin, a powerful antioxidation flavonoid, has a significant effect on ROS clearance on THP-1 macrophages after lipopolysaccharide (LPS) or LPS-combined adenosine triphosphate stimulation, thus downregulating MMP9 expression, suppressing NF-κB signaling, and interrupting the formation of the NLRP3 inflammasome. Moreover, our in vivo data showed that procyanidin attenuated Dextran sulfate sodium-induced experimental colitis in a dose-dependent fashion by suppressing the expression of MMP9, NF-κB, and NLRP3 inflammasome signaling in colonic tissues in mice. Overall, our results suggested that targeting ROS could be a potential therapeutic choice for colonic inflammation.
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BIOMEDICINE & PHARMACOTHERAPY
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2018
·
IF 3.457
Miao Yan,Zhu-feng Wu,Dan Tang,Feng Wang,Yi-wen Xiao,Ping Xu,Bi-kui Zhang,Yi-ping Liu,Da-xiong Xiang,Hoan Linh Banh
Voriconazole (VRC) and proton pump inhibitors (PPIs) have similar metabolic pathways. The objectives of the study are to evaluate the impact of PPIs on the pharmacokinetics of VRC. Human liver microsomes model was applied to assess the inhibitory effects of PPIs on the metabolism of VRC in vitro. A retrospective study was also carried out to explore the relationship between the plasma VRC trough concentrations and PPIs uses. Patients were divided into six groups: control (n = 166), lansoprazole (LAN, n = 38), esomeprazole (ESO, n = 19), omeprazole (OME, n = 45), pantoprazole (PAN, n = 43), and ilaprazole (ILA, n = 38) groups. All five PPIs showed concentration-dependent inhibitory effects on the VRC metabolism in human liver microsomes, among which LAN, OME and ESO were three of the most potent inhibitors. Consistently, co-administered with LAN, OME and ESO significantly increased the plasma VRC trough levels ( p < 0.05), whereas there was no significant association between VRC concentrations and PAN or ILA use. Interestingly, patients in the PPIs groups were more likely to reach the therapeutic VRC range of 1–5.5 μg/mL in steady state when compared with control patients (75–81% VS 69%). In conclusion, although all PPIs showed inhibitory effects on the VRC metabolism in vitro, only LAN, OME and ESO significantly increased VRC plasma concentrations. This study should be helpful for choice of the type of PPIs for patients administered with VRC.
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BMC Nephrology
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2018
·Vol. 19
·期 1
·1-12
·
IF 2.395
Yang Huan,Male Musa,Li Yang,Wang Ning,Zhao Chenming,Jin Shan,Hu Juncheng,Chen Zhiqiang,Ye Zhangqun,Xu Hua
Background Substrate reduction therapy with analogs reduces the accumulation of substrates by inhibiting the metabolic pathways involved in their biosynthesis, providing new treatment options for patients with primary hyperoxalurias (PHs) that often progress to end-stage renal disease (ESRD). This research aims to evaluate the inhibition efficacy of Hydroxy-L-proline (HYP) analogs against calcium oxalate (CaOx) crystal formation in the Drosophila Melanogaster ( D. Melanogaster ) by comparing them with Pyridoxine (Vitamin B6). Methods Three stocks of Drosophila Melanogaster (W 118 , CG3926 RNAi, and Act5C-GAL4/CyO) were utilized. Two stocks (CG3926 RNAi and Act5C-GAL4 /CyO) were crossed to generate the Act5C > dAGXT RNAi recombinant line (F 1 generation) of D. Melanogaster which was used to compare the efficacy of Hydroxy-L-proline (HYP) analogs inhibiting CaOx crystal formation with Vitamin B 6 as the traditional therapy for primary hyperoxaluria. Results Nephrolithiasis model was successfully constructed by downregulating the function of the dAGXT gene in D. Melanogaster ( P -Value = 0.0045) . Furthermore, the efficacy of Hydroxy-L-proline (HYP) analogs against CaOx crystal formation was demonstrated in vivo using D. Melanogaster model; the results showed that these L-Proline analogs were better in inhibiting stone formation at very low concentrations than Vitamin B 6 (IC 50 = 0.6 and 1.8% for standard and dietary salt growth medium respectively) compared to N-acetyl-L-Hydroxyproline (IC 50 = 0.1% for both standard and dietary salt growth medium) and Baclofen (IC 50 = 0.06 and 0.1% for standard and dietary salt growth medium respectively). Analysis of variance (ANOVA) also showed that Hydroxy-L-proline (HYP) analogs were better alternatives for CaOx inhibition at very low concentration especially when both genetics and environmental factors are intertwined ( p < 0.0008) for the dietary salt growth medium and ( P < 0.063) for standard growth medium. Conclusion Addition of Hydroxy-L-Proline analogs to growth medium resulted in the reduction of CaOx crystals formation. These analogs show promise as potential inhibitors for oxalate reduction in Primary Hyperoxaluria.
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RSC Advances
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2018
·Vol. 8
·期 43
·24633-24640
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IF 2.936
Jie Li,Suqin Shen,Fei Kong,Ting Jiang,Cui Tang,Chunhua Yin
Mesoporous silica nanoparticles (MSN) have been widely applied for drug delivery systems. To investigate the effects of pore size on anticancer efficacies, MSN with different pore sizes but similar particle sizes and surface charges were synthesized via a microemulsion method. The pore structures of MSN were characterized by transmission electron microscopy (TEM), small-angle X-ray scattering (SAXS), and N2 adsorption–desorption isotherms. Doxorubicin loaded MSN (DOX/MSN) were prepared and the minimum drug loading capacity was detected in DOX/MSN with a pore size of 2.3 nm (DOX/MSN2). DOX/MSN with a pore size of 8.2 nm (DOX/MSN8) showed a comparable drug loading amount in comparison with ones with a pore size of 5.4 nm (DOX/MSN5). In vitro drug release profiles showed that DOX/MSN5 could release DOX quickly and completely. Compared with DOX/MSN2 and DOX/MSN8, DOX/MSN5 showed the higher cellular uptake and nucleic concentration of DOX in QGY-7703 cells, which led to efficient cell-apoptosis induction and anti-proliferation effect, and thus the optimal in vivo anticancer activities. Taken together, these results highlighted the importance of pore size in anticancer efficacies, which would serve as a guideline in the rational design of MSN for cancer therapy.
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ACS Sustainable Chemistry & Engineering
·
2018
·
IF 6.14
Penghui Ding,Jun Di,Xiaoliu Chen,Mengxia Ji,Kaizhi Gu,Sheng Yin,Gaopeng Liu,Fei Zhang,Jiexiang Xia,Huaming Li
In this study, S, N codoped graphene quantum dots/(BiO)2CO3 hollow microspheres have been fabricated by a facile electrostatic self-assembly method. The nanosized S, N:GQDs, which can be obtained by a bottom-up approach, are superior surface modification materials for photocatalytic applications due to their better electron transfer and peroxidase mimetic properties. The excellent oxidation property of the synthesized nanocomposite is confirmed by degradation of different model pollutants, such as rhodamine B, tetracycline, and bisphenol A under light irradiation or dark situation. Based on several experiments, the essential roles of S, N:GQDs can be described as (i) a photocarrier transport center strengthening photoinduced charge carriers (h+–e–) separation and (ii) an enzymatic-like catalysis center to accelerate H2O2 decomposition to produce ·OH because the surface accumulation of H2O2 is harmful for photocatalytic processes. The present work may pave the way for integrating enzymatic-like cocatalysis into a photocatalytic process to generate more reactive oxygen species, thus advancing the field of environmental remediation and synthetic chemistry.
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高影响因子
MOLECULES
·
2018
·Vol. 23
·期 10
·2492
·
IF 3.098
Xiaodan Liu,Fei Liu,Weihao Huang,Jiyu Peng,Tingting Shen,Yong He
Rapid detection of Cd content in soil is beneficial to the prevention of soil heavy metal pollution. In this study, we aimed at exploring the rapid quantitative detection ability of laser- induced breakdown spectroscopy (LIBS) under the conditions of air and Ar for Cd in soil, and finding a fast and accurate method for quantitative detection of heavy metal elements in soil. Spectral intensity of Cd and system performance under air and Ar conditions were analyzed and compared. The univariate model and multivariate models of partial least-squares regression (PLSR) and least-squares support vector machine (LS-SVM) of Cd under the air and Ar conditions were built, and the LS-SVM model under the Ar condition obtained the best performance. In addition, the principle of influence of Ar on LIBS detection was investigated by analyzing the three-dimensional profile of the ablation crater. The overall results indicated that LIBS combined with LS-SVM under the Ar condition could be a useful tool for the accurate quantitative detection of Cd in soil and could provide reference for environmental monitoring.Keywords:soil;Cd;laser-induced breakdown spectroscopy;argon
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JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY A-CHEMISTRY
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2019
·
IF 3.261
Han Zhang,Mei Zhu,Dao-Yong Jiang,Xing-Ying Xue,Jiao Zhang,Guo-Ning Zhang,Yu-Cheng Wang,Hong Zhao
A NIR fluorescent probe AZ-NO 2 for hydrogen sulfide was designed and synthesized through simple method, which employs dicyanoisophorone based fluorescent dye as a fluorophore and 2,4-dinitrophenyl ether moiety as the specific reaction site. Owing to the H 2 S-induced efficient thiolysis of dinitrophenyl ether, the probe displayed excellent selectivity toward H 2 S, a low detection limit of 16.3 nM, a large Stokes shift (185 nm) and an ultrafast response time (75 s). In addition, the sensing mechanism was investigated via mass spectra. Finally, in view of its favourable biocompatibility, the probe AZ-NO 2 has been successfully applied to the fluorescent bioimaging of H 2 S in HepG2 cells and zebrafish.
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BIOSENSORS & BIOELECTRONICS
·
2019
·
IF 9.518
Tingting Xu,Jiming Li,Shaoyin Zhang,Yongcan Jin,Rui Wang
Fast and accurate detection of S. aureus and effectively kill S. aureus in the bloodstream in low doses acted as key roles in medicine and microbiology studies. In this work, sufonated-hyaluronic acid (S-HA) terminated magnetic mesoporous silica nanoparticles (MMSNs) loaded with vancomycin was successfully prepared, and S. aureus antibody was further modified on the surface of MMSNs ( [email protected] @MMSNs) by amidation reaction. The result showed that [email protected] @MMSNs were 240 nm-sized spheres with worm-like mesoporous channels. With the aid of magnetic interaction, [email protected] @MMSNs were modified on the surface of magnetic glassy carbon electrode (MGCE). This prepared immunosensor exhibited an excellent detection limit and linear range, and possessed good selectivity , stability and reproducibility. Besides, since the S-HA terminated MMSNs have anticoagulant property, the [email protected] @MMSNs/MGCE showed antiadhesion property, which ensure that it can be directly applied to detect the amount of S. aureus in whole blood. What's more, with an increasing amount of S. aureus arriving at MGCE, the capping [email protected] @MMSNs were degraded by Hyal which was secreted by S. aureus , and the packaged vancomycin was subsequently released. Ultimately, S. aureus can be effectively killed. Therefore, this integrated platform would be a promising multifunctional platform for achieving accurate diagnosis and efficient treatment of S. aureus bloodstream infection.
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高影响因子
JOURNAL OF MEMBRANE SCIENCE
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2019
·
IF 7.015
Lingling Zhang,Hui Jia,Jie Wang,Haitao Wen,Juan Li
The electrochemical impedance spectroscopy (EIS) was used to monitor the ion transport of the ion exchange membrane (IEM) interface with ectopic monitoring. But the general monitoring methods were lack of the information in concentration polarization (CP) and fouling formation. In this paper, an in-situ method for monitoring the ion mass transfer by EIS was established which can distinguish the contribution of membrane fouling and CP. The results showed that the in-situ EIS could more comprehensively monitor the desalination process. Membrane fouling, especially the formation of fouling layer on membrane surface, promoted the CP at membrane/solution interface. This was mainly because the ion mass transfer was hindered by serious membrane fouling, so that the ion concentration near the membrane surface was higher than that of the solution, and this phenomenon was gradually aggravated with the increase of membrane fouling. The recovery of membrane performances were assessed by different cleaning methods, it was found that the chemical cleaning effect was superior to inverted electrode according to online EIS. When irreversible fouling was formed in the membrane and then the fouled membrane was chemically cleaned, the formation of the fouling layer was accelerated and the CP was aggravated during membrane reuse.
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