APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
·
2018
·Vol. 102
·期 24
·10713-10727
·
IF 3.34
Wang Yanbo,Xie Qiuhong,Sun Sheng,Huang Baojia,Zhang Ying,Xu Yun,Zhang Shumin,Xiang Hongyu
Weaning stress has serious negative effects on piglets’ health and the swine industry. Probiotics-fermented Chinese herbal medicines are potential feed additives to ameliorate weaning stress. In this study, the effects of probiotics-fermented Massa Medicata Fermentata (MMFP) on intestinal homeostasis were evaluated in weaning piglets. Dietary supplementation with MMFP promoted the development of the intestinal structure and elevated the concentrations of lactic acid and short-chain fatty acids (SCFAs) in the intestinal contents and antioxidant capacities in serum. MMFP reduced the levels of inflammatory factors in the intestinal mucosa. Microbial community analysis demonstrated that MMFP led to the selective and progressive enrichment of lactic acid- and SCFA-producing bacteria along the gastrointestinal tract, in particular, OTUs corresponding to Lactobacillus , Streptococcus , Acetitomaculum , Roseburia , and Eubacterium xylanophilum group, while MMFP reduced the relative abundance of pathogenic bacteria. On the contrary, antibiotics had negative effects on intestinal histology and increased the relative abundance of pro-inflammatory bacterium, such as Marvinbryantia , Peptococcus , Turicibacter , and Blautia . Correlation analysis reflected that the bacteria enriched in MMFP group were positively correlated with enhanced intestinal homeostasis, which suggested that dietary supplementation with MMFP enhanced host intestinal homeostasis by modulating the composition of gut microbiota and the levels of beneficial SCFAs, thus ameliorating weaning stress in piglets.
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Scientific Reports
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2018
·Vol. 8
·期 1
·1-11
·
IF 4.122
Xiao Qiling,Zhou Qiongqiong,Yang Lu,Tian Zhongyuan,Wang Xiliang,Xiao Yuncai,Shi Deshi
Human carboxylesterases has been proven to be age and race-related and a sound basis of clinical medication. PLE involve in signal transduction and highly catalyze hydrolysis. Therefore, the expression level of PLE most probably exist age and breed difference and lead to significant differences of pharmacology and physiology. Four age groups of Tongcheng (TC) and Large White (LW) pigs were selected to explore PLE breed and age differences, and it was found that PLE mRNA was most abundant in liver in both breeds. In liver, PLE levels and hydrolytic activities increased with age, and PLE levels (except for 3 month) and the hydrolytic activities were higher in LW than in TC across all age groups. Abundance of PLE isoenzymes was obvious different between breeds and among age groups. The most abundant PLE isoenzyme in LW and TC pigs was PLE-A1 (all age groups) and PLE-B9 (three early age groups) or PLE-G3 (adult groups), respectively. 103 new PLE isoenzymes were found, and 55 high-frequency PLE isoenzymes were accordingly classified into seven categories (A-G). The results of this research provide a necessary basis not only for clinical medication of pigs but also for pig breeding purposes.
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JOURNAL OF SOL-GEL SCIENCE AND TECHNOLOGY
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2018
·Vol. 88
·期 3
·639-645
·
IF 1.745
Wang Yunwei,Zhao Gaoyang,Wu Chuanbao,Duan Zongfan
CaMn 7 O 12 precursor sol was prepared by using Ca(NO 3 ) 2 ·4H 2 O and Mn(CH 3 COO) 4 ·4H 2 O as the raw materials, acetylacetone (AcAcH) as the chelating agent, and methyl alcohol (MeOH) as the solvent. The CaMn 7 O 12 crystalline film was obtained via dip-coating and annealing treatment on the LaAlO 3 (001) single-crystal substrate. XRD θ -2 θ scan indicated that the as-prepared CaMn 7 O 12 film had strong preferred orientation along the c -axis. In addition, the results of the ω and ϕ scans demonstrated that the film exhibited outstanding out-of-plane and in-plane texture characteristics. The SEM characterization showed that the CaMn 7 O 12 film was dense and free of cracks. The grain size was uniform with an average size of ~180 nm. Vibrating sample magnetometer (VSM) test results indicated the CaMn 7 O 12 film was antiferromagnetic and had a saturation magnetization of 114.2 emu/cm 3 at 50 K.
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International Journal of Peptide Research and Therapeutics
·
2018
·Vol. 25
·期 4
·1361-1367
·
IF 1.132
Li Songtao,Zhao Hongling,Wang Ruxing,Wang Jianping,Mao Xiaoxia,Hao Ting,Chang Xiaomin,Zhao Enhong,Yin Zhifeng,Deng Shuhua,Yang Yaqi,Wang Huina
It is demonstrated that gonadotropin-releasing hormone (GnRH) analogs can directly inhibit the proliferation of reproductive tissue cancer cells, but the poor pharmacokinetic properties still restrict their application in treating hormone-dependent diseases. Modifications in position 6 and 10 of natural GnRH can improve the metabolic stability. In order to study the effect of incorporation Cys 6 substitution with C-terminal Pro 9 -NHEt modification and dimerization of linear peptides on metabolic stability and antiproliferative activity of GnRH analogs, two new GnRH analogs [ l -Cys 6 , desGly 10 , Pro 9 -NHEt]-GnRH ( 1 ) and [ d -Cys 6 , desGly 10 , Pro 9 -NHEt]-GnRH ( 2 ), and their corresponding dimer derivatives ([ l -Cys 6 , desGly 10 , Pro 9 -NHEt]-GnRH) 2 ( 3 ) and ([ d -Cys 6 , desGly 10 , Pro 9 -NHEt]-GnRH) 2 ( 4 ) were synthesized. Incubation of these analogs with human serum was carried out to evaluate their metabolic stability, and direct growth inhibitory effect of the two dimer derivatives 3 and 4 on MCF-7 human breast cancer cell line was examined by MTT assay. The metabolic stability of dimer derivatives 3 and 4 was remarkably improved in comparison with natural GnRH. The d -Cys 6 substituted dimer derivative 4 exhibited higher inhibitory effect (29.6–39.7% growth reduction) on cell growth than its corresponding counterpart 3 (21.8–26.2% growth reduction) at concentration range of 50, 100 and 200 μΜ. The cell growth inhibition of leuprolide was 16.4–27.2% at the tested concentrations. The dimer derivative 4 was the most stable and active GnRH analog in this study and has the potential for future preclinical investigations as promising antitumor drug candidate.
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Journal of Coatings Technology and Research
·
2018
·Vol. 16
·期 1
·25-30
·
IF 1.619
Li Pengchong,Cai Chao,Long Yuhua,Zhu Tang,Dong Haixia,Zhu Caizhen,Zhao Ning,Xu Jian
Microsized Cu 2 O particles are widely used in tin-free marine antifouling (AF) paints. Because of the enlarged specific surface area, utilization of Cu 2 O particles with smaller sizes is a potential way to improve the AF efficiency and lower the Cu 2 O content. In this paper, the clay, commonly applied as filler in the paint, was previously used to control the concentration of free Cu 2 + during the synthesis of Cu 2 O. With the help of clay, the size of obtained Cu 2 O particles decreased from 750 to 460 nm. It has been shown that the smaller-sized Cu 2 O can work against the marine biofouling with a lower content (15 wt%) compared to the commercial microsized Cu 2 O (40 wt%) in the 260 days of marine AF field tests.
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Scientific Reports
·
2019
·Vol. 9
·期 1
·1-8
·
IF 4.011
Wang Xiangnan,Liu Tao,Yu Jingkun
(4YSZ) 0.93 (Fe 2 O 3 ) 0.07 and 9 mol% Y 2 O 3 stabilized ZrO 2 (9YSZ) were synthesized by co-precipitation method and their crystalline structure, microstructure, electronic conductivity, total conductivity were characterized. A limiting current oxygen sensor was assembled with (4YSZ) 0.93 (Fe 2 O 3 ) 0.07 dense diffusion barrier and 9YSZ solid electrolyte by Pt sintered-paste method. Influences of temperature ( T ), oxygen concentration ( x (O 2 )) and water vapor pressure ( p (H 2 O)) on sensing characteristics of the limiting current oxygen sensor were investigated. The crystalline structure of (4YSZ) 0.93 (Fe 2 O 3 ) 0.07 and 9YSZ belong to cubic structure with \({\bf{Fm}}\overline{{\bf{3}}}{\bf{m}}\) . The total conductivity of (4YSZ) 0.93 (Fe 2 O 3 ) 0.07 is higher than that of 9YSZ and the electronic and total conductivities of the samples meet the linear relationship with 1000/ T . The limiting current oxygen sensor exhibits excellent sensing characteristics under test conditions. The effects of T , x (O 2 ) and p (H 2 O) are as follows: Log( I L · T ) depends linearly on 1000/ T , I L depends linearly on x (O 2 ) and I L is not significantly dependent on p (H 2 O).
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CELLULOSE
·
2019
·Vol. 26
·期 18
·9863-9875
·
IF 3.917
Wang Jinran,Wan Jinquan,Ma Yongwen,Wang Yan
Abstract The deinking of mixed office waste paper by cellulase, M/Las (modification of laccase aspartic acid system), and C-M/Las (cellulase synergistic modification of laccase aspartic acid system) was investigated. The fiber morphology parameters, hydrogen bond patterns, cellulose crystallinity, and fiber microstructure were observed via fiber quality analyzer (FQA), Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), and scanning electron microscopy (SEM). The results showed that the effective residual ink concentration of enzyme deinked pulp decreased compared with blank pulp (pulp without enzymatic treatment). The deinking efficiencies of C-M/Las, cellulase, and M/Las were 18.17%, 14.01%, and 12.31%, respectively. FQA analysis indicated that the fiber length and curl index decreased, while the fiber width slightly increased. FTIR analysis revealed that after cellulase, M/Las, and C-M/Las treatment, the content of intermolecular hydrogen bonds increased by 14.65%, 13.37%, and 19.80%, respectively. This indicates that there is a synergistic effect between cellulase and M/Las. XRD showed that the cellulose crystallinity decreased after enzymatic deinking. SEM micrographs of fibers treated with enzymes revealed that the fiber surface became rough and more fibrils appeared. The water retention value was increased after enzymatic deinking. Graphic abstract
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Journal of Inorganic and Organometallic Polymers and Materials
·
2019
·Vol. 30
·期 5
·1589-1599
·
IF 1.637
Zhao Peng,Zhang Jinteng,Jiang Jingjing,Wang Hongtao,Xie Tengfeng,Lin Yanhong
A novel Pt/CeO 2 /ZnO ternary composite is synthesized via two simple procedures of hydrothermal and photoreduction. The crystal structure, morphology, and composition of as-prepared samples are characterized by XRD, SEM, HRTEM, XPS and UV–Vis DRS. Because both ZnO and CeO 2 are good photocatalytic semiconductors, photocatalytic activities of the samples are evaluated by the degradation of phenol aqueous solution (25 mg/L). Under the strong interaction among the Pt, CeO 2 and ZnO, the maximum photocatalytic activity is observed in the Pt/CeO 2 /ZnO ternary composites and 91% phenol can be degraded in 60 min under UV light irradiation. The probable photocatalytic mechanism is discussed by active species trapping experiments along with SPV, TPV, PL and PA measurements. The enhanced photocatalytic activity is attributed to the redox cycle of Ce 4+ ↔ Ce 3+ , the effective interface between ZnO and CeO 2 as well as the electron transfer action of Pt nanoparticles. The photocatalytic activity almost unchanged after four cycles and proves excellent reusable photocatalysts. This work shows the synergistic effect of rare earth elements and noble metals in the photocatalytic process, which facilitates their practical application in toxic pollution abatement.
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JOURNAL OF ELECTRONIC MATERIALS
·
2019
·Vol. 48
·期 11
·7474-7478
·
IF 1.676
Qing Zhenjun,Li Haiyan,Zhao Chenglin
A microwave dielectric BaCu 2 V 2 O 8 ceramic with a low intrinsic sintering temperature was synthesized by a solid-state sintering process. The influences of sintering process on the crystal phase composition, microstructure, relative density, and microwave dielectric properties of BaCu 2 V 2 O 8 ceramic were studied utilizing scanning electron microscopy and an x-ray diffractometer. The crystalline phase of BaCu 2 V 2 O 8 was the only one phase found in ceramic. The sample fired at 740°C for 6 h had the optimal microwave dielectric properties ( ε r = 13.5, Q × f = 46,300 GHz and τ f = − 41.5 ppm/°C). The results mean that electronic devices fabricated by low temperature co-fired ceramic technology based on BaCu 2 V 2 O 8 ceramic have potential applications in the high-frequency field.
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MICROCHIMICA ACTA
·
2019
·Vol. 186
·期 10
·1-9
·
IF 5.479
He Maofang,Wei Yinmao,Wang Rong,Wang Chunyang,Zhang Bo,Han Lu
A boronate-modified magnetic affinity sorbent was prepared by adopting hyperbranched polyethyleneimine as the scaffold to amplify initiator sites. 3-Acrylamidophenylboronic acid was employed as monomer to proceed in situ free-radical polymerization on magnetite (Fe 3 O 4 ) nanoparticles. Due to the improved density of boronic acid polymers, the adsorbent exhibited high adsorption capacity, typically (134 ± 8) μg mg −1 for dopamine, (92 ± 7) μg mg −1 for catechol, (363 ± 14) μg mg −1 for ovalbumin and (464 ± 22) μg mg −1 for horseradish peroxidase. These capacities are much higher than those of other adsorbents. The sorbent was applied to the enrichment of catecholamines from spiked human urine. Owing to the high adsorption capacity, only 1.0 mg of adsorbent was sufficient to eliminate the interferences and enrich the targets (dopamine, norepinephrine and epinephrine) within 5 min. They were quantified by HPLC. The recoveries from spiked samples range between 83.5% ~106%, with relative standard deviations of 3.2 ~ 9.4% ( n = 5). The separation of glycoproteins from egg white was also accomplished prior to their analysis by PAGE. In the authors’ perception, this approach is promising in that the density of functional groups on the adsorbent is strongly increased.
The preparation routine of boronate affinity magnetic adsorbent (Fe 3 O 4 @HpAAPBA). The adsorbent is used for the magnetic solid phase extraction of cis -dol compounds from real sample.
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Nanoscale Research Letters
·
2019
·Vol. 14
·期 1
·1-9
·
IF 3.159
Liu Zhi,Ju Kaiyi,Wang Zongqian,Li Wei,Ke Huizhen,He Jihuan
Electrical voltage has a crucial effect on the nanofiber morphology as well as the jet number in the electrospinning process, while few literatures were found to explain the deep mechanism. Herein, the electrical field distribution around the spinning electrode was studied by the numerical simulation firstly. The results show that the electrical field concentrates on the tip of a protruding droplet under relatively low voltage, while subsequently turns to the edge of needle tip when the protruding droplet disappears under high voltage. The experimental results are well consistent with the numerically simulated results, that is, only one jet forms at low voltage (below 20 kV for PVDF-HFP and PVA nanofiber), but more than one jet forms under high voltage (two jets for PVDF-HFP nanofiber, four jets for PVA nanofiber). These more jets lead to (1) higher fiber diameter resulting from actually weaker electrical field for each jet and (2) wide distribution of fiber diameters due to unstable spinning process (changeable jet number/site/height) under high voltage. The results will benefit the nanofiber preparation and application in traditional single-needle electrospinning and other electrospinning methods.
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Tissue Engineering and Regenerative Medicine
·
2019
·Vol. 16
·期 6
·573-583
·
IF 2.272
Zhao Yang,Sun Qing,Wang Shurong,Huo Bo
Background: Mesenchymal stem cells (MSCs) have strong self-renewal ability and multiple differentiation potential. Some studies confirmed that spreading shape and area of single MSCs influence cell differentiation, but few studies focused on the effect of the circularity of cell shape on the osteogenic differentiation of MSCs with a confined area during osteogenic process. Methods: In the present study, MSCs were seeded on a micropatterned island with a spreading area lower than that of a freely spreading area. The patterns had circularities of 1.0 or 0.4, respectively, and areas of 314, 628, or 1256 μm 2 . After the cells were grown on a micropatterned surface for 1 or 3 days, cell apoptosis and F-actin were stained and analyzed. In addition, the expression of β-catenin and three osteogenic differentiation markers were immunofluorescently stained and analyzed, respectively. Results: Of these MSCs, the ones with star-like shapes and large areas promoted the expression of osteogenic differentiation markers and the survival of cells. The expression of F-actin and its cytosolic distribution or orientation also correlated with the spreading shape and area. When actin polymerization was inhibited by cytochalasin D, the shape-regulated differentiation and apoptosis of MSCs with the confined spreading area were abolished. Conclusion: This study demonstrated that a spreading shape of low circularity and a larger spreading area are beneficial to the survival and osteogenic differentiation of individual MSCs, which may be regulated through the cytosolic expression and distribution of F-actin.
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