EUROPEAN JOURNAL OF PHARMACOLOGY
·
2022
·
IF 5.195
Huizhen Sun,Anyong Zhang,Yanchun Gong,Wei Sun,Baiyi Yan,Shuihong Lei,Li-Hua Yao
Diabetes mellitus (DM) has recently become one of the major diseases that have received attention. Cordycepin (molecular formula: C 10 H 13 N 5 O 3 ), is one of the major bioactive components of Cordyceps militaris , decreases blood glucose levels. In this study, the effect and mechanism of cordycepin in normal and oxidative-damaged INS-1 cells were explored by using cell and molecular biology methods. Results showed that cordycepin could enhance insulin synthesis and secretion. The mechanism is possibly related to the elevated ATP content induced membrane depolarisation and increased Ca 2+ concentration. At the genetic level, cordycepin upregulated the mRNA level of insulin, pancreatic duodenal homeobox factor-1 (PDX-1) and glucose transporter 1 (GLUT1). At the protein level, cordycepin promoted the expression of PDX-1, GLUT1, serine threonine kinase (Akt) and phosphorylated Akt (P-Akt). These effects may also contribute to the enhancement of insulin synthesis and secretion. Further analysis revealed that cordycepin protected against H 2 O 2 -induced damage on INS-1 cells and improved their viability and insulin synthesis/secretion. This effect should be attributed to the reduced intracellular reactive oxygen species (ROS), enhanced mitochondrial membrane potential (MMP), increased activity of superoxide dismutase (SOD) and upregulated genetic and protein expression of catalase (CAT), PDX-1, GLUT1 and P-Akt. In conclusion, cordycepin promotes insulin synthesis and secretion in normal islet β cells and improves this function in oxidative-damaged islet β cells. Given that islet β cells are vulnerable to oxidative stress , the improving effect of cordycepin on the antioxidant capacity and insulin synthesis/secretion of INS-1 cells may be an important mechanism for its hypoglycaemic effect.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
SEPARATION AND PURIFICATION TECHNOLOGY
·
2022
·
IF 9.136
Fei Yu,Xueting Bai,Mingxing Liang,Jie Ma
Considering the increasingly serious levels of antibiotic pollution, it is necessary to find new and efficient adsorbents. In this study, the adsorptive removal of a model quinolone antibiotic, ciprofloxacin (CIP), from wastewater is presented using a metal–organic framework (MOF)-derived carbon. HKUST-1-derived carbon (HDC-1100) was obtained by high-temperature carbonization of the HKUST-1 precursor at 1100 °C. HDC-1100 has a regular octahedron shape decorated with Cu/Cu 2 O particles and has a microporous and mesoporous structure with abundant oxygen-containing functional groups on the surface. Within 48 h, HDC-1100 could reach adsorption equilibrium for 30 mg·L -1 CIP, and the kinetic data were fitted with a pseudo-first-order model and a pseudo-second-order model. In this study, the maximum adsorption capacity of HDC-1100 for CIP was as high as 592 mg·g −1 . The high adsorption capacity is attributed mainly to metal–organic complexation, electrostatic interactions, and hydrogen bonding. Also, the effects of environmental factors, such as pH, ionic strength, and humic acid, on the adsorption capacity were considered. This study is significant for the treatment of high-concentration antibiotic-polluted wastewater.
✓ 已验证
产品关联
DOI
高影响因子
Small Methods
·
2022
·Vol. 6
·期 4
·2101509
·
IF 15.367
Xiaojie Zhou,Yichen Cai,Mingsheng Xu,Jianping Li,Chenxu Sheng,Qiuyi Zhang,Xinxia Qiu,Wenchong Wang,Shisheng Xiong,Chunxiao Cong,Zhi-Jun Qiu,Ran Liu,Laigui Hu
The emergence of near-eye displays, such as head-mounted displays, is triggering a requirement for highly enhanced display resolution. High-resolution micro-displays with micro-organic light-emitting diodes (micro-OLEDs) can be a preferential candidate, owing to the mature industrialization of OLEDs along with the advantages of flexibility, light weight, and ease of processing. However, micro-OLEDs with pixel sizes down to micrometers are difficult to be achieved using conventional techniques such as fine metal mask evaporation and lithography. Here, a solution-processing approach to pattern organic semiconductors (OSCs) for micro-OLED arrays with the assistance of templated dewetting is demonstrated. Solvents containing organic functional materials are dewetted on the surface with hydrophobic/hydrophilic patterns to form ordered droplet arrays using dip-coating. Subsequently, patterned OSC films are produced by effectively controlling solvent evaporation. Micro-OLED arrays with a pixel size down to 1 µm are successfully fabricated by further deposition of emitting/electron transport layers and top electrodes. This approach can open an avenue for low-cost manufacturing of flexible and high-resolution micro-displays.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Journal of Materials Chemistry B
·
2022
·Vol. 10
·期 9
·1386-1392
·
IF 7.571
Ling Yang,Ping’an Ma,Xiaorui Chen,Ziyong Cheng,Jun Lin
Sensitive detection of circulating tumor DNA (ctDNA) in vitro has attracted growing attention owing to its potential application in diagnostics of cancer. In this study, we synthesized hydrophilic AgInS2@ZnS core–shell quantum dot nanocrystals and magnetic Fe3O4 nanoparticles, and then the ctDNA triggered hybridization chain reaction was used to detect the CYFRA21-1 DNA associated with lung cancer. In the presence of CYFRA21-1 DNA, three hairpin structures were activated to turn on successively, resulting in the accumulation of quantum dots and eliciting considerable changes of the fluorescence signal. Compared with the conventional fluorescence detection, Fe3O4 provides magnetic adsorption properties and a large surface area for immobilizing and aggregating quantum dot nanoparticles attached to single-stranded DNA. The concentration of CYFRA21-1 is closely related to the number of quantum dots remaining after magnetic adsorption, which provides a promising approach for ctDNA quantification.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Frontiers in Immunology
·
2022
·
IF 8.786
Gu Lingui,Sun Mingjiang,Li Ruihao,Zhang Xingyu,Tao Yihao,Yuan Ye,Luo Xu,Xie Zongyi
Neuroinflammation has been proven to exert an important effect on brain injury after intracerebral hemorrhage (ICH). Previous studies reported that Didymin possessed anti-inflammatory properties after acute hepatic injury, hyperglycemia-induced endothelial dysfunction, and death. However, the role of Didymin in microglial pyroptosis and neuroinflammation after ICH is unclear. The current study aimed to investigate the effect of Didymin on neuroinflammation mediated by microglial pyroptosis in mouse models of ICH and shed some light on the underlying mechanisms. In this study, we observed that Didymin treatment remarkably improved neurobehavioral performance and decreased BBB disruption and brain water content. Microglial activation and neutrophil infiltration in the peri-hematoma tissue after ICH were strikingly mitigated by Didymin as well. At the molecular level, administration of Didymin significantly unregulated the expression of Rkip and downregulated the expression of pyroptotic molecules and inflammatory cytokines such as Nlrp3 inflammasome, GSDMD, caspase-1, and mature IL-1β, TNF-α, and MPO after ICH. Besides, Didymin treatment decreased the number of Caspase-1-positive microglia and GSDMD-positive microglia after ICH. Inversely, Locostatin, an Rkip-specific inhibitor, significantly abolished the anti-pyroptosis and anti-neuroinflammation effects of Didymin. Moreover, Rkip binding with Asc could interrupt the activation and assembly of the inflammasome. Mechanistically, inhibition of Caspase-1 by VX-765 attenuated brain injury and suppressed microglial pyroptosis and neuroinflammation by downregulation of GSDMD, mature IL-1β, TNF-α, and MPO based on Locostatin-treated ICH. Taken together, Didymin alleviated microglial pyroptosis and neuroinflammation, at least in part through the Asc/Caspase-1/GSDMD pathway via upregulating Rkip expression after ICH. Therefore, Didymin may be a potential agent to attenuate neuroinflammation via its anti-pyroptosis effect after ICH.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Frontiers in Oncology
·
2023
·
IF 4.7
Liu Zhenfeng,Wen Guanghua,Huang Yuqiao,Dong Yanzhao,Wang Zewei,Alhaskawi Ahmad,Zhang Shuyi,Wang GuoLin,Ye Qianni,Zhou Haiying,Lu Hui,Dong Mengjie
Background: The cell adhesion molecule (CAM) N-cadherin has become an important target for tumor therapy. The N-cadherin antagonist, ADH-1, exerts significant antitumor activity against N-cadherin-expressing cancers.Methods: In this study, [18F]AlF-NOTA-ADH-1 was radiosynthesized. An in vitro cell binding test was performed, and the biodistribution and micro-PET imaging of the probe targeting N-cadherin were also studied in vivo.Results: Radiolabeling of ADH-1 with [18F]AlF achieved a yield of up to 30% (not decay-corrected) with a radiochemical purity of >97%. The cell uptake study showed that Cy3-ADH-1 binds to SW480 cells but weakly binds to BXPC3 cells in the same concentration range. The biodistribution results demonstrated that [18F]AlF-NOTA-ADH-1 had a good tumor/muscle ratio (8.70±2.68) in patient-derived xenograft (PDX) tumor xenografts but a lower tumor/muscle ratio (1.91±0.69) in SW480 tumor xenografts and lowest tumor/muscle ratio (0.96±0.32) in BXPC3 tumor xenografts at 1 h post-injection (p.i.) These findings were in accordance with the immunohistochemistry results. The micro PET imaging results revealed good [18F]AlF-NOTA-ADH-1 tumor uptake in pancreatic cancer PDX xenografts with strong positive N-calcium expression, while lower tumor uptake in SW480 xenografts with positive expression of N-cadherin, and significantly lower tumor uptake in BXPC3 xenografts with low expression of N-cadherin, which was consistent with the biodistribution and immunohistochemistry results. The N-cadherin-specific binding of [18F]AlF-NOTA-ADH-1 was further verified by a blocking experiment involving coinjection of a non radiolabeled ADH-1 peptide, resulting in a significant reduction in tumor uptake in PDX xenografts and SW480 tumor.Conclusion: [18F]AlF-NOTA-ADH-1 was successfully radiosynthesized, and Cy3-ADH-1 showed favorable N-cadherin-specific targeting ability by in vitro data. The biodistribution and microPET imaging of the probe further showed that [18F]AlF-NOTA-ADH-1 could discern different expressions of N-cadherin in tumors. Collectively, the findings demonstrated the potential of [18F]AlF-NOTA-ADH-1 as a PET imaging probe for non-invasive evaluation of the N-cadherin expression in tumors.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ACS Omega
·
2023
·
IF 4.1
Guoqiang Tang,Yichuan Li,Zhaosheng Yang,Yu Wang,Guangyun Li,Yilou Wang,Yong-Ming Chai,Chenguang Liu
Titanium silica (TS-1) membrane catalysts grown on the surfaces of spherical substrates can both exploit the high catalytic performance and facilitate their separation from products after the reaction. In this work, a simple static crystallization method was used to perform the in situ construction of a TS-1 membrane on the surfaces of micron-sized spherical carriers. The shortcomings of the TS-1 membrane under static crystallization conditions were overcome by in situ dynamic crystallization, and the effect of rotation speed on the formation of the molecular sieve membrane was investigated. The results showed that the molecular sieve membrane was smooth and homogeneous, with a higher synthesis efficiency at a slow rotational speed. The micron TS-1 spherical membrane catalytic chloropropene epoxidation reaction was investigated in a fixed bed, and the conversion of hydrogen peroxide and selectivity of epichlorohydrin reached 99.4 and 96.8%, respectively. After being reused twice, the catalyst still maintained a stable catalytic performance.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
MICROCHIMICA ACTA
·
2023
·Vol. 190
·期 8
·1-9
·
IF 5.7
Hao Xuanxuan,Liu Zhimin,Fan Yunfeng,Wang Jie,Cui Chen,Hu Leqian
A novel electrochemiluminescence (ECL) aptasensor, using graphite carbonitride (g-C 3 N 4 ) capped CdS quantum dots (CdS QDs@g-C 3 N 4 ) and Au nanoparticles decorated triethanolamine (AuNPs@TEOA) as dual coreactants, was proposed for the determination of mucin 1 (MUC1). Higher ECL efficiency was acquired due to the double enhancement contribution of CdS QDs and TEOA to Ru (bpy) 3 2+ ECL. Additionally, AuNPs@TEOA also acted as nanocarrier for MUC1 aptamer immobilization. After the aptasensor was incubated in target MUC1, the decreased ECL emission was obtained because of the poor conductivity of MUC1. The ECL aptasensor displayed a good linear correlation for MUC1 in the range 0.1 pg mL −1 -1000 ng mL −1 , and the detection limit was 33 fg mL −1 . MUC1 spiked into human serum samples was quantified to assess the practicability of the ECL aptasensor. Graphical abstract
✓ 已验证
产品关联
DOI
PubMed
高影响因子
SEPARATION AND PURIFICATION TECHNOLOGY
·
2023
·
IF 8.6
Siyu Pang,Zhihao Si,Yan Zhuang,Hanzhu Wu,Jan Baeyens,Xinmiao Zhang,Peiyong Qin
Polydimethylsiloxane (PDMS) is the benchmark pervaporation (PV) material, and its hollow fiber (HF) membrane is generally fabricated by thermal curing, which is energy-intensive and hard to be controlled. For the first time, a continuous and controllable photo-curing system for the fabrication of hydrophobic PDMS HF membrane is proposed. In this process, the uncoated PVDF HF membrane firstly exits from the feed roll, and enters into the tank filled with photo-cured PDMS solution. After being coated by PDMS, the new liquid layer uninterruptedly enters into the UV irradiation module and realizes the photo-curing reaction. Finally, the cured PDMS HF membrane moves into the take-up roll for collection. Due to this technology firstly being assessed, a series of operation parameters are optimized, including 126, 507 g mol −1 of the PDMS molecular weight, 12 wt% of PDMS concentration in its coating solution, 20.1 mW cm −2 of the irradiation intensity, 0.86 m min −1 of the rotation speed of roll and 1 time of the number of dip-coating. Under these parameters, the prepared PDMS HF membrane achieves an outstanding separation factor of 18.2 with a total flux of 1821 g m −2 h −1 for PV separation of 1-butanol. Our study has proven the feasibility of photo-cured fabrication of PDMS HF membranes for 1-butanol pervaporation.
✓ 已验证
DOI
高影响因子
JOURNAL OF MEMBRANE SCIENCE
·
2023
·
IF 9.5
Hongyong Zhao,Ting Song,Xiaoli Ding,Ruyi Cai,Xiaoyao Tan,Yuzhong Zhang
Membrane technology for O 2 /N 2 separation is a mature industrial unit. Membranes with excellent permeation-separation performance are still one of the critical requirements. The cobalt-based compounds have been well known for their good affinity for O 2 , which can facilitate O 2 transfer and improve the O 2 /N 2 selectivity in membranes. The cobalt-based compounds, moreover, have a magnetic responsive effect, which can lead to a further improvement in O 2 /N 2 separation under a magnetic field. A class of PIM-1 mixed matrix membranes (MMMs), containing cobalt-based ionic liquid (CILs)@polyarylate (aromatic polyester) (PAR) (core-shell) composite nanospheres (CILs@PAR (c-s) CNPs), were prepared. Chemical compositions of CILs@PAR (core-shell) CNPs were determined by Fourier transform infrared spectroscopy (FTIR). Morphologies and structures of CILs@PAR (core-shell) CNPs were observed by transmission electron microscopy (TEM). Gas adsorption properties of CILs@PAR (core-shell) CNPs were tested. Thermal properties of the (CILs@PAR (c-s) CNPs)/PIM MMMs were investigated by simultaneous thermal analysis (TGA-DSC). Gas permeation-separation performances of the (CILs@PAR (c-s) CNPs)/PIM MMMs were also reported. Compared with the original PIM-1 membrane, the (CILs@PAR (c-s) CNPs)/PIM MMMs display higher O 2 /N 2 selectivity because of cobalt-based ionic liquid as O 2 carrier, coupled with a reduction in O 2 permeability. With increasing the content of the CILs@PAR (c-s) CNPs, the O 2 /N 2 selectivity increases, and the O 2 permeability decreases. It is also found that, as a magnetic field on the MMMs, the O 2 /N 2 selectivity further increases; and it increases more, as a higher content of CILs@PAR (c-s) CNPs. Therefore, the (CILs@PAR (c-s) CNPs)/PIM MMMs exhibit excellent O 2 /N 2 selectivity of ∼5 coupled with good O 2 permeability of ∼140 Barrer under a 180 mT magnetic field and show great potential for air separation.
✓ 已验证
产品关联
DOI
高影响因子
PLASMA CHEMISTRY AND PLASMA PROCESSING
·
2023
·Vol. 43
·期 6
·1963-1978
·
IF 3.6
Lv Huan,Liu Xinyuan,Hao Yingzi,Yi Yanhui
The industrial utilization of methane to methanol is based on the indirect pathway with syngas as an intermediate product, but the reaction conditions are very harsh and the costs in equipment and operations are extremely high. Therefore, the direct oxidation of methane to methanol (DOMTM) under mild conditions is more desirable. In this paper, coupling of non-thermal plasma and Cu–zeolite (Cu–ZSM-5 and Cu–S-1) catalysts have been studied for DOMTM. The effect of Si/Al ratios (Cu–ZSM-5 and Cu–S-1 catalysts) on the reaction performance has been investigated. Cu–S-1 catalyst exhibited the best catalytic performance, i.e., 5.8% methane conversion and 50.6% methanol selectivity. The employed catalysts have been characterized by XRD, UV–Vis, XPS and FT-IR. The results show that Cu–ZSM-5 catalysts with high Si/Al ratio are conducive to the formation of isolated Cu 2+ species, and Cu–S-1 catalyst exhibits the most abundant isolated Cu 2+ species. Furthermore, Cu–S-1 catalyst shows the most abundant O ads species. The plentiful surface silicon defects on S-1 is the reason why Cu–S-1 catalyst shows the most abundant isolated Cu 2+ and O ads species, and these two species are interdependent to form a Si–O–Cu species, which may be the active site for DOMTM. Based on these findings, the possible catalytic mechanism was proposed.
✓ 已验证
产品关联
DOI
高影响因子
ENVIRONMENTAL TECHNOLOGY
·
2021
·
IF 3.247
Min Xiaobo,Li Xinyu,Zhao Jie,Hu Xiaoxian,Yang Weichun
Aiming at exploring an effective photocatalytic adsorbent for organic dye removal, a series of heterostructured TiO2@HKUST-1 photocatalysts, by incorporating HKUST-1 with different TiO2 nanoparticles loading, were prepared by single-step hydrothermal method. The morphology and surface characteristics of the as-prepared TiO2@HKUST-1were analyzed using SEM, HRTEM, XRD, FTIR, UV–vis DRS, and Photoluminescence techniques. The adsorption-photocatalytic degradation of the model dye methylene blue (MB) on the catalysts was investigated. It was indicated that the introduction of a certain amount of TiO2 on the surface of HKUST-1 could improve the transfer and separation of the photogenerated charge carriers, resulting in the enhanced photocatalytic activity. The optimal 0.02TiO2@HKUST-1 exhibited the highest MB removal rate with about 4.4 and 19.3 times as high MB removal efficiency as that of HKUST-1 and TiO2, respectively. Heterostructured TiO2@HKUST-1 materials for the removal of MB involved the integrated adsorption and visible light photocatalysis. Meanwhile, the composite exhibited good reusability in the process of cyclic experiments. Therefore, this work provides a potential MOF-based photocatalytic adsorbent for organic dye removal.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）