Food and Bioprocess Technology
·
2019
·Vol. 12
·期 10
·1741-1755
·
IF 3.032
Jiang Lei,Belwal Tarun,Huang Hao,Ge Zhiwei,Limwachiranon Jarukitt,Zhao Yechao,Li Li,Ren Guoping,Luo Zisheng
The extraction and characterization of bioactive compounds from bamboo shoot shell was performed under ultrasonic-assisted extraction (UAE) along with UPLC-Triple-TOF/MS analysis. Under optimized UAE conditions (ethanol concentration (58%), liquid to solid ratio (24 mL/g), extraction temperature (59 °C), and sonication time (29 min)), total phenolic content (TPC) was recorded highest as 85.3 mg GAE/g DW, while DPPH and FRAP antioxidant activities were 77 μmol AAE/g DW and 33 μmol AAE/g DW, respectively. Using UPLC-Triple-TOF/MS analysis, fifteen phenolic acids, seven flavonoids, nineteen organic acids, two iridoid glucosides, and one neoglinan were identified. Among them, p -coumaric acid (119 μg/g DW), chlorogenic acid (87 μg/g DW), rutin (39 μg/g DW), and ferulic acid (17 μg/g DW) were found to be the most abundant phenolic compounds. Furthermore, compounds including phenolic acids (3 -p- coumaroylquinic acid, cryptochlorogenic acid, 3 -O- feruloylquinic acid, 5 -O- feruloylquinic acid, 3 -O- caffeoylshikimic acid, 5 -p- coumaroylquinic acid, 1,3-dicaffeoyl quinic acid, 3,5-dicaffeoyl quinic acid), flavonoids (schaftoside/c-hexosyl-c-pentosylapigenin, apigenin 6,8-di-C-α- l -arabinopyranoside, kaempferide 3 -O- α- l -rhamnopyranosyl(1->6)-β- d -glucopyranoside, 6-C-β- d -glucopyranosyl-8-C-α- l -arabinopyranosylchrysin, narcissin), some organic acids, iridoid glucosides, and neolignan were also detected for the first time from bamboo shoot shell extract. The present study revealed that bamboo shoot shell extract contains a larger number of nutraceutically active compounds which can be further utilized by food and nutraceutical industries.
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JOURNAL OF NANOBIOTECHNOLOGY
·
2019
·Vol. 17
·期 1
·1-11
·
IF 5.345
Hou Zhanwu,Wang Zhen,Liu Run,Li Hua,Zhang Zhengyi,Su Tian,Yang Jeffy,Liu Huadong
Background Gold nanoparticles (AuNPs) have been proposed for many applications in medicine and bioanalysis. For use in all these applications, maintaining the stability of AuNPs in solution by suppressing aggregation is paramount. Herein, the effects of amino acids were investigated in stabilizing AuNPs by rationally designed peptide scaffolds.Results Compared to other tested amino acids, phosphotyrosine (pY) significantly stabilized AuNPs. Our results indicated that pY modified AuNPs presented a high level of stability in various solutions, and had good biocompatibility. When a pY-peptide was used in stabilizing AuNPs, the phosphate group could be removed by phosphatases, which subsequently caused the aggregation and the cargo release of AuNPs. In vitro study showed that AuNPs formed aggregation in a phosphatase concentration depending manner. The aggregation of AuNPs was well correlated with the enzymatic activity (R2 = 0.994). In many types of cancer, a significant increase in phosphatases has been observed. Herein, we demonstrated that cancer cells treated with pY modified AuNPs in conjunction with doxorubicin killed SGC-7901 cells with high efficiency, indicating that the pY peptide stabilized AuNPs could be used as carriers for targeted drug delivery.Conclusion In summary, pY peptides can act to stabilize AuNPs in various solutions. In addition, the aggregation of pY-AuNPs could be tuned by phosphatase. These results provide a basis for pY-AuNPs acting as potential drug carriers and anticancer efficacy.
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PubMed
高影响因子
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
·
2019
·Vol. 26
·期 28
·28860-28870
·
IF 2.914
Sun Zhiming,Yuan Fang,Zhang Xinchao,Zhu Rui,Shen Xinyi,Sun Bingyan,Wang Bin
Nowadays, most of mineral-based adsorbents are powder form, which makes them inconvenient to collect and always easy to cause secondary pollution. In this work, an organic rectorite composite nanofiber membrane (SRt-PAN) was designed and prepared by electrospinning technique. The as-received composite nanofiber membranes were characterized by XRD and SEM analysis, proving the homodisperse and existence of SRt in PAN nanofiber membrane. A series of batch experiments for BPA adsorption were carried out to investigate the effect of different adsorption parameters, including initial concentration, pH, and temperature of pollutant solution. The influences of modifier dosage and adsorbent dosage on the adsorption performance were investigated as well. On the basis of the experiment results, the adsorption process could be well described by the pseudo-second-order model and the Langmuir isotherm. In addition, the thermodynamic parameters indicate that this adsorption process is exothermic and spontaneous. Moreover, compared with pure nanofiber membranes and organic rectorite powders, the resultant SRt-PAN adsorbents exhibited higher adsorption capacity, superior reusability, and adsorption stability. It is indicated that the hydrophobicity surface of organic rectorite should be the key factor to not only the intimate interfacial combination between the mineral and PAN, but also the enhancement of BPA adsorption capacity.
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PubMed
高影响因子
CHEMISTRY AND TECHNOLOGY OF FUELS AND OILS
·
2018
·Vol. 53
·期 6
·966-975
·
IF 0.36
Cao Cheng,Pu Xiaolin,Wang Gui,Huang Tao
The properties of conventional shale hydration inhibitors were studied using samples of Longmaxi shale. The inhibition was evaluated by the rolling recovery test, linear swelling rate, and clay interlayer spacing. The results indicate that K 2 SiO 3 inhibits clay hydration far better than other inorganic shale inhibitors while polyamine is superior to other organic shale inhibitors of clay hydration. Alkaline solution is more effective in suppressing shale dispersion. A neutral environment has little impact on this parameter. On the contrary, an acidic medium can even promote it. Most of the inhibitors are incapable of suppressing simultaneously clay dispersion and expansion. Therefore, they should be used in conjunction to suppress synergistically clay hydration, dispersion, and expansion.
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ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
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2018
·Vol. 25
·期 23
·22455-22463
·
IF 2.8
Wang Pingping,Dong Faqin,Liu Mingxue,He Huichao,Huo Tingting,Zhou Lei,Zhang Wei
Photoelectrochemical process is an environmentally friendly technology and has a wide application in the control of environmental pollutants. Efficient nanophotocatalysts responsive to visible light are still highly attractive. In this work, α-Fe 2 O 3 /TiO 2 were grown on fluorine doped tin oxide (FTO) substrates by hydrothermal method for photoelectrochemical reduction of Cr(VI). Compared with the separate α-Fe 2 O 3 and TiO 2 electrodes, the composite α-Fe 2 O 3 /TiO 2 electrodes show higher photocurrent density. Under visible light irradiation, 100% removal efficiency of Cr(VI) was obtained after 40 min treatment. The composite α-Fe 2 O 3 /TiO 2 electrodes showed an enhanced absorbance in visible light region and had good stability to photoelectrochemical reduction of Cr(VI). The role of hole scavengers (citric acid and oxalic acid) and pH values was systematically investigated. This novel intensification approach provides new insight on the application of photoelectrochemical reduction in environmental remediation.
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高影响因子
Scientific Reports
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2018
·Vol. 8
·期 1
·1-12
·
IF 4.122
Farooq Muhammad U.,Novosad Valentyn,Rozhkova Elena A.,Wali Hussain,Ali Asghar,Fateh Ahmed A.,Neogi Purnima B.,Neogi Arup,Wang Zhiming
Colloidal gold nanoparticles (AuNPs) are of interest as non-toxic carriers for drug delivery owing to their advanced properties, such as extensive surface-to-volume ratio and possibilities for tailoring their charge, hydrophilicity and functionality through surface chemistries. To date, various biocompatible polymers have been used for surface decoration of AuNPs to enhance their stability, payloads capacity and cellular uptake. This study describes a facile one-step method to synthesize stable AuNPs loaded with combination of two anticancer therapeutics, -bleomycin and doxorubicin. Anticancer activities, cytotoxicity, uptake and intracellular localization of the AuNPs were demonstrated in HeLa cells. We show that the therapeutic efficacy of the nanohybrid drug was strongly enhanced by the active targeting by the nanoscale delivery system to HeLa cells with a significant decrease of the half-maximal effective drug concentration, through blockage of HeLa cancer cell cycle. These results provide rationale for further progress of AuNPs-assisted combination chemotherapy using two drugs at optimized effective concentrations which act via different mechanisms thus decreasing possibilities of development of the cancer drug resistance, reduction of systemic drug toxicity and improvement of outcomes of chemotherapy.
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高影响因子
JOURNAL OF FOOD SCIENCE AND TECHNOLOGY-MYSORE
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2018
·Vol. 55
·期 3
·964-976
·
IF 1.797
Zhang Guanfei,Chen Wenxue,Chen Weijun,Chen Haiming
Matured coconut water (MCW) is a by-product in the coconut milk industry that is usually discarded due to its unpleasant flavor. In this study, low-alcohol coconut water (LACW) was fermented with Saccharomyces cerevisiae to improve the quality of MCW. Volatile components and nonvolatile flavor-related elements were estimated to compare the qualities of the MCW and LACW. Besides measuring the kinetic changes, the levels of fructose, glucose, sucrose and ethanol contents were also determined. The results of the organic acid assays showed that tartaric, pyruvic and succinic acids were the primary organic acids present in LACW and increased significantly with fermentation. The resulting volatile composition assay indicated that esters, alcohols and fatty acids were significantly influenced by fermentation and yeast strains. Moreover, 1,1-diphenyl-2-picrylhydrazyl (DPPH), 2,2′-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS), cupric ion reducing antioxidant capacity and ferric reducing antioxidant power values increased significantly throughout the process, correlating with the enhancement of total phenolic content.
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高影响因子
TRANSITION METAL CHEMISTRY
·
2018
·Vol. 43
·期 3
·259-271
·
IF 1.261
Liu Ya-Xian,Mo Hui-Wen,Lv Zhen-Yu,Shen Fang,Zhang Chun-Lian,Qi Yong-Yu,Mao Zong-Wan,Le Xue-Yi
A copper complex [Cu(HPBM)( l -Phe)(H 2 O)]·ClO 4 (1) (HPBM = 5-methyl-2-(2′-pyridyl)benzimidazole, l -Phe = l -phenylalanine anion) was synthesized and characterized by elemental analysis, IR, ESI–MS, HR–ESI–MS, ESR spectroscopy, and by X-ray single-crystal analysis. The binding constant of the complex with calf thymus DNA (CT-DNA) was determined as 7.38 (± 0.57) × 10 4 M −1 . Further studies indicated that the complex interacts with CT-DNA through minor groove binding. The in vitro cytotoxic activities of both the free proligand and the complex against Eca-109, HeLa and A549 cancer cells and normal LO2 cells were evaluated by the MTT method. The IC 50 values range from 5.7 ± 0.1 to 8.3 ± 0.6 µM. Free HPBM displays no cytotoxic activity against the selected cancer cells, with IC 50 values more than 100 µM. Double staining analysis showed that the complex can induce apoptosis in Eca-109 cells. Comet assays demonstrated that the complex can damage DNA and cause apoptosis. The complex also induces an increase in intracellular reactive oxygen species and a reduction in mitochondrial membrane potential. The complex can also increase the intracellular Ca 2+ level and induce release of cytochrome c . The cell cycle arrest was investigated by flow cytometry. The results demonstrate that the complex induces apoptosis in Eca-109 cells through DNA-binding and ROS-mediated mitochondrial dysfunctional pathways.
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JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY
·
2018
·
IF 2.869
Bing Xia,Yuanji Gao,Baocheng Ji,Fengwei Ma,Lisheng Ding,Yan Zhou
Electrospray ionization mass spectrometry (ESI-MS) technique has limitations in analysis of compounds that are dissolved in nonpolar solvents. In this study, ambient ionization of compounds in solvents that are not “friendly” to electrospray ionization, such as n-hexane, is achieved by conductive nanomaterials spray ionization (CNMSI) on nanomaterial emitters, including carbon nanotubes paper and mesodendritic silver covered metal, which applies high voltages to emitters made of these materials without the assistance of polar solvents. Although the time intensity curves (TIC) commonly vary from 4.5% to 23.7% over analyses, protonated molecular ions were found to be the most abundant species, demonstrating good reproducibility of the technique in terms of ionized species. Higher mass spectrometric responses are observed in analyzing nonpolar systems than polar systems. 2-Methoxyacetophenone, 4-methylacetophenone, benzothiazole, quinolone, and cycloheptanone as low as 2 pg in n-hexane can be directly detected using the developed method. The developed technique expands the analysis capability of ESI-MS for direct, online analysis of nonpolar systems, such as low polarity extracts, normal phase liquid chromatography eluates, and synthetic mixtures.
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高影响因子
JOURNAL OF POLYMERS AND THE ENVIRONMENT
·
2018
·Vol. 26
·期 7
·2945-2953
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IF 1.971
Chen Huayao,Lin Guanquan,Zhou Hongjun,Zhou Xinhua,Xu Hua,Huang Suqing
Avermectin (AVM) is a highly efficient pesticide against a variety of insects and is widely used in agriculture. However, it is susceptible to oxidation and photolysis, resulting in instability under UV irradiation and a short half-life. Herein, sodium carboxymethyl cellulose (CMC) was grafted by styrene (St), Methyl methacrylate (MMA) and butyl acrylate (BA) respectively to prepare grafted polymer nanoparticles in application for AVM sustained released. And the influence of different grafted monomer and the grafted rate on the AVM/grafted polymer nanoparticles performance was discussed. The larger the grafted rate of St was, the larger the particle size of AVM/grafted polymer nanoparticles had with a higher drug loading rate and stronger resistance to ultraviolet light. Besides, the particle size, drug loading rate and the ability of anti-ultraviolet light of grafted CMC emulsion was also influenced by the modifying agent. While the concentration of AVM was kept the same, the acrylic esters (MMA and BA) grafted emulsion have smaller particle sizes with higher drug loading rates and stronger anti-ultraviolet ability comparing to styrene (St) grafted emulsion. The effect of different amount of St and different monomer grafted on the sustained release performance of AVM/grafted polymer was also investigated. As the grafted rate of St increased, the release rate became smaller. The release speed has a sequence of CMC-g-PBA > CMC-g-PS > CMC-g-PMMA when CMC grafted with different monomer. Their sustained release curves can be described by Korsmeyer-Peppas equation. Finally, the insect toxicity test showed that insecticide toxicity against diamondback moth ( Plutella xylostella L.) has no apparent difference among the AVM and AVM/grafted polymer nanoparticles indicating that AVM/grafted polymer nanoparticles can reduce the amount of organic solution used for dissolution and prolonged its service life without decreasing its insecticide toxicity.
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TRANSITION METAL CHEMISTRY
·
2018
·Vol. 43
·期 2
·149-159
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IF 1.261
Chen Jincan,Zhang Yao,Li Baojun,Li Guodong,Jie Xinming,Cui Ying,Zou Zhilin,Huang Xiaofeng,Qu Jiaoyue,Chen Lanmei
Two ruthenium complexes [Ru(MeIm) 4 (bpy)] 2+ ( Ru1 , MeIm = 1-methylimidazole, bpy = 2,2′-bipyridine) and [Ru(Im) 4 (bpy)] 2+ ( Ru2 , Im = imidazole) with the same PF
− 6
counter-ion but different lipophilicities were synthesized and characterized and as potent anticancer agents. The relationships between cellular uptake, localization and molecular action mechanisms of these complexes were elucidated. The results showed that Ru1 with higher log P o/w exhibited faster cellular uptake rates, but lower anticancer activity than Ru2 . In addition, Ru1 predominantly accumulated in the mitochondria and cytoplasm, and induced G0/G1 cell cycle arrest, whereas the more hydrophilic Ru2 tended to localize and accumulate in the cell nucleus and mitochondria. Further mechanism studies indicated that Ru2 caused cell cycle arrest at S phase by regulating cell cycle related proteins and induced apoptosis in A549 cells through DNA damage, cellular ROS accumulation, activation of the caspase pathway and mitochondrial dysfunction.
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IONICS
·
2018
·Vol. 24
·期 10
·3053-3063
·
IF 2.347
Qaisrani Naeem Akhtar,Ma Yanjiao,Ma Lingling,Liu Jiafei,Gao Li,Li Lv,Gong Shoutao,Yan Xiaoming,Zhang Fengxiang,He Gaohong
A new type of composite anion-exchange membrane is fabricated using benzoxazine (Bz) monomer and polytetrafluoroethylene (PTFE) via a green and facile method. Membrane fabrication is achieved via in situ ring-opening polymerization of Bz within the PTFE matrix, followed by quaternization and ion-exchange reactions. The quaternized PBz works as a self-cross-linked and anion conductive polymer. The synthesized membranes show improved conductivity (26 to 70 mS/cm) at a reasonable water uptake and a low swelling ratio; they also show improved alkaline stability for 150 h at 60 °C in 1 M KOH solution, the decrease in conductivity being only ca. 10%. Our method of AEM fabrication is advantageous over conventional ones due to facile process and the avoidance of chloro- or bromomethylation as well as the self-cross-linked structure; the resulting membranes show relatively good performance as compared with some of those obtained from conventional techniques.
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