APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
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2019
·Vol. 189
·期 2
·608-625
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IF 2.14
Zhou Ruolin,Ren Zhiqing,Ye Jun,Fan Yawei,Liu Xiaoru,Yang Jianyuan,Deng Ze-Yuan,Li Jing
Soybean dregs fermented by Neurospora crassa is a typical traditional food in Gannan district of China. In this study, in vitro imitated gut fermentation was carried out to evaluate whether the oligosaccharides from this fermented soybean dregs had potential prebiotic properties. 11.91% of oligosaccharides were extracted from the fermented soybean dregs at the optimized condition which of 1:25 for ratio of soybean dregs (g) to 50% ethanol (ml), 90 min of extracted duration at 70 °C for twice. The soybean dreg oligosaccharides (SBOS) were progressively purified with Sevag method and on columns filled with AB-8 macroporous resin, and then identified as cellobiose by HPLC-ESI-MS and FT-IR. Oligosaccharides of soybean dregs with 800 mg/L significantly decreased pH value ( p < 0.05) and ammonia N concentration ( p < 0.05), and increased short chain fatty acid (SCFA) level ( p < 0.05) in imitated gut fermentation compared with control group. It was shown that this fermented soybean dregs could be a potential prebiotic food.
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ANALYTICAL AND BIOANALYTICAL CHEMISTRY
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2019
·Vol. 411
·期 14
·3081-3089
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IF 3.286
Zhang Ying,Ren Wang,Fan Yu Zhu,Dong Jiang Xue,Luo Hong Qun,Li Nian Bing
In this work, a facile and label-free ratiometric sensor is constructed for selective determination of norepinephrine (NE) by coupling second-order scattering (SOS) and fluorescence, two different and independent optical signals. Herein, polyethyleneimine (PEI) dilute solution medium shows an intensive SOS signal without any fluorescence response. Interestingly, NE can be selectively induced by PEI to emit bright fluorescence, and meanwhile causes an observable decrease in the SOS signal due to the interactions between NE and PEI. The simultaneous variation of the two independent signals can be used for ratiometric sensing of NE. Under the optimal conditions, the resultant ratiometric sensor displays high sensitivity and selectivity toward NE by simultaneously monitoring fluorescence and SOS signals with the same excitation wavelength. The proposed sensor exhibits a good linear relationship versus NE concentration in the range of 10.0 nM–45.0 μM with a detection limit of 2.0 nM ( S / N = 3) and has been successfully applied to the determination of NE in real samples without the use of any extra reagent. The combination of fluorescence and SOS signals provides a new scheme for ratiometric sensor design, greatly simplifying experimental procedure and effectively enhancing detection accuracy. Moreover, the proposed analytical strategy further broadens the application of dilute solutions of polymers in research into optical sensor and green analytical chemistry.
Graphical abstract
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Nature Communications
·
2018
·Vol. 9
·期 1
·1-7
·
IF 12.353
Lei Zhouyue,Wu Peiyi
Biomimetic skin-like materials, capable of adapting shapes to variable environments and sensing external stimuli, are of great significance in a wide range of applications, including artificial intelligence, soft robotics, and smart wearable devices. However, such highly sophisticated intelligence has been mainly found in natural creatures while rarely realized in artificial materials. Herein, we fabricate a type of biomimetic iontronics to imitate natural skins using supramolecular polyelectrolyte hydrogels. The dynamic viscoelastic networks provide the biomimetic skin with a wide spectrum of mechanical properties, including flexible reconfiguration ability, robust elasticity, extremely large stretchability, autonomous self-healability, and recyclability. Meanwhile, polyelectrolytes’ ionic conductivity allows multiple sensory capabilities toward temperature, strain, and stress. This work provides not only insights into dynamic interactions and sensing mechanism of supramolecular iontronics, but may also promote the development of biomimetic skins with sophisticated intelligence similar to natural skins.
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Scientific Reports
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2017
·Vol. 7
·期 1
·1-11
·
IF 4.259
Huang Haiying,Tao Xuanyu,Jiang Yiming,Khan Aman,Wu Qi,Yu Xuan,Wu Dan,Chen Yong,Ling Zhenmin,Liu Pu,Li Xiangkai
Soil contamination by PAH and heavy metals is a growing problem. Here, we showed that a new isolate, Pseudomonas brassicacearum strain LZ-4, can simultaneously degrade 98% of 6 mM naphthalene and reduce 92.4% of 500 μM hexavalent chromium [Cr (VI)] within 68 h. A draft genome sequence of strain LZ-4 (6,219,082 bp) revealed all the genes in the naphthalene catabolic pathway and some known Cr (VI) reductases. Interestingly, genes encoding naphthalene pathway components were upregulated in the presence of Cr (VI), and Cr (VI) reduction was elevated in the presence of naphthalene. We cloned and expressed these naphthalene catabolic genes and tested for Cr (VI) reduction, and found that NahG reduced 79% of 100 μM Cr (VI) in 5 minutes. Additionally, an nahG deletion mutant lost 52% of its Cr (VI) reduction ability compared to that of the wild-type strain. As nahG encodes a salicylate hydroxylase with flavin adenine dinucleotide (FAD) as a cofactor for electron transfer, Cr (VI) could obtain electrons from NADH through NahG-associated FAD. To the best of our knowledge, this is the first report of a protein involved in a PAH-degradation pathway that can reduce heavy metals, which provides new insights into heavy metal-PAH contamination remediation.
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Scientific Reports
·
2017
·Vol. 7
·期 1
·1-10
·
IF 4.259
Chen Meiling,Frueh Johannes,Wang Daolin,Lin Xiankun,Xie Hui,He Qiang
Hydromorphic biological systems, such as morning glory flowers, pinecones, and awns, have inspired researchers to design moisture-sensitive soft actuators capable of directly converting the change of moisture into motion or mechanical work. Here, we report a moisture-sensitive poly( p -phenylene benzobisoxazole) nanofiber (PBONF)-reinforced carbon nanotube/poly(vinyl alcohol) (CNT/PVA) bilayer soft actuator with fine performance on conductivity and mechanical properties. The embedded PBONFs not only assist CNTs to form a continuous, conductive film, but also enhance the mechanical performance of the actuators. The PBONF-reinforced CNT/PVA bilayer actuators can unsymmetrically adsorb and desorb water, resulting in a reversible deformation. More importantly, the actuators show a pronounced increase of conductivity due to the deformation induced by the moisture change, which allows the integration of a moisture-sensitive actuator and a humidity sensor. Upon changing the environmental humidity, the actuators can respond by the deformation for shielding and report the humidity change in a visual manner, which has been demonstrated by a tweezer and a curtain. Such nanofiber-reinforced bilayer actuators with the sensing capability should hold considerable promise for the applications such as soft robots, sensors, intelligent switches, integrated devices, and material storage.
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Journal of Food Measurement and Characterization
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2024
·
IF 2.9
Yu Qian,Yang Yonglin
A label-free electrochemical aptasensor was developed using a 3D porous chitosan-reduced graphene oxide nanocomposite matrix for rapid and ultrasensitive quantification of enrofloxacin residues in mutton samples. The high conductivity nanoscaffold allowed enhanced aptamer loading yielding improved dynamic range of 0.01-10 ng/mL and low detection limit of 0.005 ng/mL. The biosensor demonstrated good precision (RSD 2.7%) between measurements and selectivity against potentially interfering species. It also showed excellent accuracy with 92.7–97.5% recovery of spiked enrofloxacin in meat extracts. A storage stability of 91% was retained over 4 weeks without noticeable performance loss. Further, analysis of actual mutton samples using the aptasensor revealed enrofloxacin levels of 6.8 ng/g and 15.2 ng/g, validated by HPLC-UV reference technique. Thus, the proposed label-free impedimetric aptasensor provides a viable analytical platform for rapid on-site screening of trace enrofloxacin residues in complex food matrices within minutes, without requiring extensive sample processing steps or expensive instrumentation. The biosensor fabrication methodology exploiting advanced nanomaterials to tune sensitivity shows promise for enabling rapid detection of diverse targets.
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RESEARCH ON CHEMICAL INTERMEDIATES
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2024
·
IF 2.8
Yu Qingbo,Li Xianhua
The dicyandiamide/phthalic acid (DCDA/PA) precursor with molecular assembly strategy was prepared by hydrothermal treatment the mixture of dicyandiamine and phthalic acid, in which some of the amines were bound by phthalic acid through hydrogen bonding interactions, and the carbon nitride (CN) photocatalyst was further prepared by thermal polymerization. The results showed that the new chemical structure formed by the interaction of melamine cyanurate and phthalic acid through hydrogen bonding could hinder the intermolecular collision chances during the calcination process of DCDA/PA precursor, which made the polymerization reaction difficult to be carried out, and the CN photocatalysts with irregular layered stacking structure were formed. In addition, the presence of more amino groups in the CN-0.3 structure can effectively improve the separation efficiency of its photogenerated charge carriers, and after simulated visible light irradiation for 60 min, the photocatalytic activity presented by CN-0.3 for MB was 2.72 times higher than that of pristine g-C 3 N 4 .
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JOURNAL OF THERMAL ANALYSIS AND CALORIMETRY
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2024
·Vol. 149
·期 24
·14715-14725
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IF 3
Chen Peng,Chen Liping,Zhou Juan,Wu Wenqian,Guo Zichao,Chen Wanghua
Thermal analysis and calorimetry techniques with sealed sample cells are frequently employed to investigate the thermal stability of hazardous materials. However, the influence of sample mass on thermal decomposition is often ignored. To investigate the effect of sample mass on autocatalytic decomposition substances under sealed condition, 2,4-DNT was chosen for test and analysis. The techniques including differential scanning calorimetry (DSC), rapid screening calorimetry(RSC), high-performance liquid chromatography-mass spectrometry (HPLC–MS), and gas chromatography-mass spectrometry (GC–MS) were utilized for in-depth research on the decomposition of 2,4-DNT. The results revealed that an increase in sample mass led to an significant elevation in gaseous products such as CO 2 and CO with a negative heat of formation (ΔH f ). Then the system pressure and decomposition heat of 2,4-DNT increase significantly, thereby enhancing the thermal decomposition process. After that, it has been determined that o–nitrotoluene, m–nitroaniline, and o–nitrobenzoic acid can catalyze the decomposition of 2,4-DNT. Finally, it is advisable to increase the sample mass as much as possible when testing and analyzing such nitro compounds which would help ensure that the predicted results are closer to the actual conditions of production, transport, storage.
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Biomass Conversion and Biorefinery
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2025
·
IF 4.1
Cui Yong,Wang Wenliang,Xu Xiaolu,Miao Hui,Fu Yishuai
In order to increase the yield of high-value components in bio-oil from waste wood-based panels (WWPs), a composite catalyst of SiC@HZSM-5 with both excellent capacities of microwave absorption and catalytic conversion was successfully prepared and applied to the microwave-assisted pyrolysis (MAP) with microwave power of 1000 W (2.45 GHz). The effects of different Si/Al ratios of HZSM-5 on the characterization of catalysts and selective pyrolysis products also have been investigated. The results showed that the obtained SiC@HZSM-5 catalyst exhibited a stable composite structure, favorable dielectric loss characteristics, and high thermal conductivity. Compared with the non-catalytic experiment, SiC@HZSM-5 catalysts significantly enhanced the relative content of nitrogen-containing compounds and reduced the concentration of oxygen-containing compounds in bio-oil, though there was one slight negative impact on bio-oil yield. Importantly, with the increase in Si/Al ratio from 30 to 90, the production of nitrogen-containing compounds was found to decrease from 37.11 to 23.14%, due to the reduction of acidic sites in catalysts. It was noteworthy that during the pyrolysis of WWPs by microwave heating, the catalyst with the Si/Al ratio of 30 significantly promoted the interactions between lignocellulosic components and adhesives and improved the selectivity of amide-N compounds (14.06%, acetamide being the main products) and heterocyclic-N compounds (23.05%, pyrroles, pyridines, and pyrazines constituted the major composition). This work could offer a promising technical reference to thermally converted WWPs to high value-added chemicals by microwave-assisted catalytic pyrolysis (MACP).
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JOURNAL OF MATERIALS SCIENCE
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2024
·Vol. 59
·期 37
·17531-17544
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IF 3.5
Zhao Shifeng,Ou Yanwen,Zhao Yanping,Liu Yuqin,Lv Fengzhu
Ferroan brucite with different Fe 2+ doping amounts (Mg 1-X Fe X (OH) 2 , x = 0.3, 0.4, 0.5, 0.6, 0.7 and 0.8) was synthesized for the removal of Congo red (CR) from wastewater. As the Fe 2+ content increased, the removal efficiency initially increased and then decreased, and Mg 0.6 Fe 0.4 (OH) 2 exhibited the maximum removal amount which was 2745.96 mg/g. The high removal ability of Mg 0.6 Fe 0.4 (OH) 2 was derived from the combined removal mechanism. The removal amount of CR was highly related to the amount of Fe 2+ in MgFe(OH) 2 and its existed state. The removal behaviors of M 0.7 Fe 0.3 (OH) 2 (M = Zn, Ni, Co) were also conducted to verify the function and university of Fe 2+ in the degradation process. The influence of M 2+ in M 0.7 Fe 0.3 (OH) 2 (M = Zn, Ni, Co) on the removal efficiency was explored by experimental study and calculation of the system energy and bond length change based on molecular simulation. This work provided an outstanding approach for preparation of high efficiency materials to purify organic wastewater. Graphical Abstract
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ACTA PHARMACOLOGICA SINICA
·
2025
·
IF 8.4
Cheng Wei-ting,Pei Si-ya,Wu Jie,Wang Yan-jie,Yang Yong-wen,Xiao Mei-fang,Chen Jun,Wang Yuan-yuan,Wu Li,Huang Ze-bing
Non-alcoholic fatty liver disease (NAFLD) is the hepatic manifestation of the metabolic syndrome. NAFLD encompasses a spectrum of liver damage starting with liver steatosis and lipid disorders presented as the hallmark. Cannabinoid-2 receptor (CB2R) is the receptor of endocannabinoids mainly expressed in immune cells. Our preliminary study revealed the preventative role of CB2R in liver injury related to lipid metabolism. In this study, we aimed to explore the role of CB2R in NAFLD and the underlying mechanism related to microbial community. High-fat diet-induced NAFLD model was established in mice. We found that hepatic CB2R expression was significantly reduced in NAFLD mice and CB2R –/– mice fed with normal chow. Interestingly, cohousing with or transplanted with microbiota from WT mice, or treatment with an antibiotic cocktail ameliorated the NAFLD phenotype of CB2R –/– mice. The gut dysbiosis in CB2R –/– mice including increased Actinobacteriota and decreased Bacteroidota was similar to that of NAFLD patients and NAFLD mice. Microbial functional analysis and metabolomics profiling revealed obviously disturbed tryptophan metabolism in NAFLD patients and NAFLD mice, which were also seen in CB2R –/– mice. Correlation network showed that the disordered tryptophan metabolites such as indolelactic acid (ILA) and xanthurenic acid in CB2R -/- mice were mediated by gut dysbiosis and related to NAFLD severity indicators. In vitro and in vivo validation experiments showed that the enriched tryptophan metabolites ILA aggravated NAFLD phenotypes. These results demonstrate the involvement of CB2R in NAFLD, which is related to gut microbiota-mediated tryptophan metabolites. Our findings highlight CB2R and the associated microbes and tryptophan metabolites as promising targets for the treatment of NAFLD.
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NAUNYN-SCHMIEDEBERGS ARCHIVES OF PHARMACOLOGY
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2024
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IF 3.1
Sun Hui,Wang ZiTong,Tu BingHua,Shao ZiChen,Li YiDan,Han Di,Jiang YinJie,Zhang Peng,Zhang WeiChang,Wu YunYan,Wu XiaoMing,Liu Chi-Ming
Type 2 diabetes mellitus (T2DM) is a metabolic disease. Diabetes increases the risk of benign prostatic hyperplasia (BPH). Capsaicin is extracted from chili peppers and possesses many pharmacological properties, including anti-diabetic, pain-relieving, and anti-cancer properties. This study aimed to investigate the effects of capsaicin on glucose metabolism and prostate growth in T2DM mice and uncover the related mechanisms. Mice model of diabetes was established by administering a high-fat diet and streptozotocin. Oral administration of capsaicin for 2 weeks inhibited prostate growth in testosterone propionate (TP)-treated mice. Furthermore, oral administration of capsaicin (5 mg/kg) for 2 weeks decreased fasting blood glucose, prostate weight, and prostate index in diabetic and TP-DM mice. Histopathological alterations were measured using hematoxylin & eosin (H&E) staining. The protein expression of 5α-reductase type II, androgen receptor (AR), and prostate-specific antigen (PSA) were upregulated in diabetic and TP-DM mice, but capsaicin reversed these effects. Capsaicin decreased the protein expression of p-AKT, insulin-like growth factor-1 (IGF-1), IGF-1R, and the receptor for advanced glycation end products (RAGE) in diabetic and TP-DM mice. Capsaicin also regulated epithelial-mesenchymal transition (EMT) and modulated the expression of fibrosis-related proteins, including E-cadherin, N-cadherin, vimentin, fibronectin, α-SMA, TGFBR2, TGF-β1, and p-Smad in TP-DM mice. In this study, capsaicin alleviated diabetic prostate growth by attenuating EMT. Mechanistically, capsaicin affected EMT by regulating RAGE/IGF-1/AKT, AR, and TGF-β/Smad signalling pathways. These results provide with new therapeutic approach for treating T2DM or T2DM-induced prostate growth.
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