Acta Biomaterialia
·
2021
·
IF 8.947
Xinran Song,Yuxin Jiang,Weixing Zhang,Gomaa Elfawal,Kaili Wang,Di Jiang,Huoyan Hong,Jinglei Wu,Chuanglong He,Xiumei Mo,Hongsheng Wang
Transcutaneous immunization (TCI) has the advantages of safety, high efficiency, non-invasiveness and convenient use. The key for a TCI system is transdermal targeted delivery of antigen to dendritic cells (DCs), the most powerful antigen presenting cells . DCs also play an important role in tumor immunotherapy, which provides a huge imagination for the application of TCI to tumor treatment. In this study, a transcutaneous tumor vaccine (TTV) delivery system was developed using the electrospun silk fibroin (SF) and polyvinyl alcohol (PVA) composite nanofibrous patch loaded with mannosylated polyethyleneimine (PEI man )-modified ethosome (Eth) (termed Eth-PEI man ). Eth-PEI man showed a good performance in targeting DCs, and the carriers loaded with antigen (encapsulated in Eths) and adjuvant (absorbed in PEI man ) were observed effectively induce DCs maturation in vitro. With the tyrosinase-related protein-2 (TRP2) peptide as antigen and oligodeoxynucleotides containing unmethylated CpG motifs as adjuvant, the TTV-loaded patches (TTVP) significantly inhibited the growth of melanoma in a syngeneic mouse model for melanoma by subcutaneous injection of B16F10 cell lines. Moreover, the combined application of the TTVP and anti-programmed death-1 monoclonal antibody (aPD-1) produced a synergistic antitumor effect, which could be related to the infiltration of more CD4 + and CD8 + T cells in the tumor tissues. The application of TTVP also increased the expression of IL-12, which may be part of the mechanism of synergistic antitumor effect between the TTVP and aPD-1. These results suggest that the combination of the TTVP and immune checkpoint blockers could be an effective strategy for tumor treatment. Statement of significance Transcutaneous immunization has the advantages of safety, high efficiency, non-invasiveness and convenient use. In this study, a novel transcutaneous tumor vaccine patch (TTVP) was developed using tumor antigens-loaded ethosomes that can target dendritic cells percutaneously. Our data demonstrated that the TTVP can significantly inhibit tumor growth. Furthermore, the combination of TTVP and aPD-1 produced a synergistic anti-melanoma effect. Considering its convenience and non-invasiveness, this TTVP system could find good application prospects in immunotherapy. The combination of TTVP and aPD-1 could be a useful strategy for the prevention and treatment of tumors.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2021
·
IF 7.46
Liyan Zhang,Xiaoting Liu,Nan Zhang,Xianqiang Liu,Wei Jiang
Sensitive and accurate detection of flap endonuclease 1 (FEN1) is essential to understand its roles in DNA replication and repair and explore its functions in tumor diagnosis and prognosis. Here, we designed a magnetic separation-assisted cascade hybridization chain reaction (HCR) amplification strategy for enzyme-free and sensitive detection of FEN1. Firstly, the forked dsDNAs contained the recognition site (5′ overhanging DNA flap) of FEN1 were modified on magbeads surface to form magnetic recognition probes. By the recognition and catalytic cleavage of FEN1, the 5′ flaps were removed into solution and then purified by magnetic separation. Then, the obtained flaps served as triggers to activate the linear-assembly of hairpin probes H1 and H2 (primary HCR), forming nicked double helices with split fragments. Finally, the fragments acted as new triggers to activate the branched-assembly of hairpin probes H3 and H4 (secondary HCR), causing fluorophore/quencher labeled on H3 far away and fluorescence recovery. Benefiting from the purification of triggers by magnetic separation and the signal amplification effect of cascade HCR, this strategy achieved a low detection limit of 3.6 × 10 −5 U μL −1 for FEN1, and enabled the assay of it in actual samples. The results demonstrate that our strategy will provide a potent approach for sensitive detection of FEN1 in early tumor diagnosis and prognosis.
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF SOLID STATE CHEMISTRY
·
2021
·
IF 3.498
Hanfang Liu,Yu Wang,Tiantian Ye,Fupeng Wang,Saisai Ran,Huijie Xie,Jia Liu,Yichuan Li,Bin Li,Yanru Liu,Yongming Chai,Lei Wang
Nanosized titanium silicalite-1 (TS-1) demonstrates excellent catalytic ability in the selective catalytic oxidation reaction. However, their synthesis process is usually complicated with low yield under hydrothermal conditions, which is not in line with the concept of green chemistry. Herein, via fully utilizing untreated seeds solution, we report firstly an entirely green strategy for solvent-free synthesizing anatase-free nanosized TS-1 zeolite . The success lies in the fully utilization of seeds solution which is composed of supersaturated structure directing agent (TPAOH), unreacted silica source, water and formed MFI seeds (silicalite-1) without external purification. In the followed solvent-free synthesis of final nanosized TS-1 product, no additional TPAOH is added, which greatly reduces the synthesis cost and synthetic procedure and maintains a high product yield. The obtained nanosized TS-1 zeolite without anatase phase has high crystallinity , large specific surface area. More importantly, the nanosized TS-1 (Si/Ti = 77) catalysts exhibit excellent catalytic ability for the epoxidation of chloropropene with 40.0% conversion and 97.6% selectivity. This sustainable and green synthesis method opens up a new way to regulate nanosized zeolite.
✓ 已验证
产品关联
DOI
高影响因子
Catalysis Science & Technology
·
2023
·Vol. 14
·期 3
·590-597
·
IF 5
Guanglu Xia,Jianhao Qiu,Dingliang Dai,Yong Tang,Zhonghao Wu,Jianfeng Yao
A covalent organic framework (COF) named TpPa-1 was co-modified by chlorine grafting and ZnIn2S4 integration for concurrent photocatalytic H2O2 generation and wastewater purification under visible light. The introduction of electron-withdrawing chlorine groups on TpPa-1 decreases the density of adjacent electron clouds, which promotes the charge carrier separation and the intimate combination with ZnIn2S4 by Cl–Zn/Cl–In bonds, providing an appreciably enhanced photocatalytic activity. Specifically, in pure water, the optimal ZnIn2S4/TpPa-Cl2 sample can achieve a H2O2 yield of 766 μmol L−1, which is 1.5, 2, 7.8 and 5 times those of ZnIn2S4/TpPa-1, TpPa-Cl2, ZnIn2S4 and TpPa-1, respectively. In the tetracycline solution, 95% of tetracycline was degraded and, concomitantly, H2O2 with a yield of 846 μmol L−1 was generated. It is found that H2O2 generation undergoes a two-step O2 reduction with ·O2− as the intermediate, while ·O2−, holes and ·OH are the active species in tetracycline degradation. Likewise, other common pollutants like o-nitrophenol, acid golden yellow and acid red in wastewater could also be effectively degraded along with the appreciable production of H2O2, which realizes the so-called wastewater valorization. This study could advance COF-based photocatalysts and provide a novel insight into wastewater treatment.
✓ 已验证
产品关联
DOI
高影响因子
Antioxidants
·
2026
·Vol. 15
·期 9
·1217
·
IF 8.2
Yuhong Huang,Meijing Wang,Yiming Zhou,Jiali Liu,Lili Ni,Ya Tan,Mengting Zhang,Jiangpeng Huang,Guolin Li,Fang Wei
Aging is a major risk factor for chronic diseases, driving an urgent need for effective geroprotectors. Repurposing psychotropic agents offers an efficient strategy to accelerate anti-aging therapeutics development. In this study, we investigated the effects of pimozide (a diphenylbutylpiperidine-class antipsychotic) on aging inCaenorhabditis elegansand elucidated the underlying mechanisms. The results demonstrated that 0.1 μM pimozide significantly extends nematode lifespan, increasing mean, median, and maximum lifespans by 44.3%, 50.0%, and 39.8%, respectively. Furthermore, pimozide improves healthspan, as evidenced by reduced age-related lipofuscin accumulation, preserved locomotor function, and decreased paralysis incidence. Moreover, pimozide-treated nematodes showed enhanced resistance to heat, ultraviolet and oxidative stress, concomitant with reduced endogenous reactive oxygen species levels. Transcriptomic profiling and biochemical validation reveal that pimozide markedly upregulates collagen gene expression and increases hydroxyproline content, indicating enhanced collagen synthesis. Mechanistically, pimozide promotes the nuclear localization of SKN-1, and its beneficial effects on lifespan extension and collagen synthesis are SKN-1-dependent, as these effects are abolished inskn-1mutants. Collectively, our findings demonstrate that pimozide promotes longevity and healthspan by activatingskn-1, which is accompanied by enhanced collagen synthesis, providing a preclinical proof of concept for its potential repurposing as a geroprotector.
✓ 已验证
DOI
PubMed
高影响因子
PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY
·
2023
·
IF 4.7
Haitong Wu,Haoye Li,Haihua Huo,Xinrun Li,Heyun Zhu,Lijiao Zhao,Jianzhao Liao,Zhaoxin Tang,Jianying Guo
Terbuthylazine (TBA) is one of the most commonly used and effective herbicides. However, due to its affinity for soil organic matter and water solubility, TBA can lead to biological health concerns. This study exposed broilers to TBA (0 mg/kg bw, 0.4 mg/kg bw, 4 mg/kg bw) for 28 days. The results showed significant pathological damage in broiler myocardial tissue, such as widening of the interstitial space, rupture of muscle fibers, and deposition of myocardial collagen fibers. In addition, Under the 0.4 mg/kg bw TBA exposure, myocardial oxidative stress was observed in broilers, which was accompanied by the activation of Nrf2/HO-1 pathway and the increased protein and mRNA levels of NQO1, NOX2 and SOD2 antioxidant enzymes . However, Nrf2/HO-1 protein and mRNA levels were reversed at 4 mg/kg bw TBA exposure. Meanwhile, the Nrf2/HO-1 mediated antioxidant defense was impaired. In contrast with the low dose, the protein and gene expression levels of NQO1, NOX2, and SOD2 were reduced in 4 mg/kg bw TBA group. The expression of GPX4 and SLC7A11 was significantly downregulated at both protein and mRNA levels. Beyond that, ACSL4 expression was significantly up-regulated, and the protein result was consistent with the mRNA expression, demonstrating the occurrence of ferroptosis . In general, TBA exposure activated the Nrf2/HO-1 pathway, resulting in ferroptosis . This study links ferroptosis to the Nrf2/HO-1 pathway, providing new insights into the potential role of TBA in myocardial toxicity.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF COLLOID AND INTERFACE SCIENCE
·
2023
·
IF 9.9
Chaobo Feng,Bing-Qiang Lu,Yunshan Fan,Haijian Ni,Yunfei Zhao,Shuo Tan,Zhi Zhou,Lijia Liu,Jordan A. Hachtel,Demie Kepaptsoglou,Baohu Wu,Denis Gebauer,Shisheng He,Feng Chen
Amorphous inorganic solids are traditionally isotropic, thus, it is believed that they only grow in a non-preferential way without the assistance of regulators, leading to the morphologies of nanospheres or irregular aggregates of nanoparticles . However, in the presence of (ortho)phosphate (Pi) and pyrophosphate ions (PPi) which have synergistic roles in biomineralization , the highly elongated amorphous nanowires (denoted ACPPNs) form in a regulator-free aqueous solution (without templates, additives, organics, etc). Based on thorough characterization and tracking of the formation process (e.g., Cryo-TEM, spherical aberration correction high resolution TEM, solid state NMR , high energy resolution monochromated STEM-EELS), the microstructure and its preferential growth behavior are elucidated. In ACPPNs, amorphous calcium orthophosphate and amorphous calcium pyrophosphate are distributed at separated but close sites. The ACPPNs grow via either the preferential attachment of ∼2 nm nanoclusters in a 1-dimension way, or the transformation of bigger nanoparticles, indicating an inherent driving force-governed process. We propose that the anisotropy of ACPPNs microstructure, which is corroborated experimentally, causes their oriented growth. This study proves that, unlike the conventional view, amorphous minerals can form via oriented growth without external regulation, demonstrating a novel insight into the structures and growth behaviors of amorphous minerals.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2023
·
IF 8.2
Xiaopeng Huang,Fangfang Wang,Wenkai Hu,Zhiming Zou,Qun Tang,Heping Li,Lin Xu
There is at present an acute need for the construction of biopolymer-based smart packaging material that can be applied for the real-time visual monitoring of food freshness. Herein, a nano-sized substituted imidazolate material (SIM-1) with ammonia-sensitive and antibacterial ability was effectively manufactured and then anchored within corn starch/polyvinyl alcohol (CS/PVA) blend to construct biopolymeric smart active packaging material. The structure, physical and functional performances of CS/PVA-based films with different content of SIM-1 (0.5, 1.0 and 2.0 wt% on CS/PVA basis) were then explored in detail. Results revealed that the incorporated SIM-1 nanocrystals were equally anchored within the CS/PVA matrix owing to the establishment of potent hydrogen-bonding interactions, which produced an obvious improvement in the compatibility of CS/PVA blend film, as well as its mechanical strength , water/oxygen barrier and UV-screening performances. The constructed CS/PVA/SIM-1 blend films further demonstrated superior long-term color stability property, ammonia-sensitive and antibacterial functions. Furthermore, the CS/PVA/SIM-1 blend films were utilized for effectively monitoring the deterioration of shrimp via observable color alteration. The above findings suggested the potential applications of CS/PVA/SIM-1 blend films in smart active packaging.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
MICROCHEMICAL JOURNAL
·
2023
·
IF 4.8
Hongyu Gan,Yaowen Liang,Yun Tang,Manna Huang,Yiqian Wan
A practical electrochemical method for the determination of the inhibitory activity of New Delhi metallo-β-lactamase-1 (NDM-1) inhibitors was established with a bare pencil graphite electrode (PGE) as the working electrode and imipenem as the substrate. To validate the method, the well-known NDM-1 inhibitors EDTA and L -captopril were selected for the corresponding assays with IC 50 values of 1.22 ± 0.20 μM and 22.92 ± 1.19 μM, respectively. These results were consistent with those (5.48 ± 0.32 μM for EDTA and 69.13 ± 6.01 μM for L- captopril) from the UV–vis high-throughput assay method. Further investigation regarding the steady kinetics of a typical competitive inhibitor of L -captopril showed that the two methods provided K i values at the same level, i.e., they are 41.5 ± 3.8 μM (DPV-PGE) and 52.5 ± 2.5 μM (UV–vis), respectively.
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF MOLECULAR LIQUIDS
·
2023
·
IF 6
Zixuan Xue,Pengrui Zhang,Qian Zhang,Chaochi Huang,Ciming Wang,Lianjing Mao,Xiaolong Zhou,Huiqun Ju,Jinhe Sun,Fei Shao,Xingwu Zou,Bo Li,Mixiang Qi,Yongzhong Jia,Yan Jing
This paper reported a novel chelating agent and a green precipitation-extraction separation strategy for lithium isotope enrichment. 1-Hydroxy-4-(p-toluidino) anthraquinone and anisole were used as chelating agent and diluent, respectively. Three phases including organic phase, aqueous phase and precipitation could be preparated. The experiment indicated that excess sodium hydroxide was helpful to promote the single-stage precipitation rate and equilibrium time. The isotope result indicated that 6 Li was concentrated in the aqueous phase and 7 Li was concentrated in precipitation phase. The maximum single-stage lithium isotope speration factor reached to 1.013 ± 0.001. By doing thermodynamic analysis of the reaction, the free energy change (ΔG), enthalpy change (ΔH) and entropy change(ΔS) of the separation process were −8.97 J mol −1 , −21.37 J mol −1 and −0.0438 J mol −1 K −1 at 283 K. The results of DFT simulation and wave function analysis indicated that a lithium ion combined with a chelating agent anion formed a stable six-member ring between two oxygen, which was more stable than sodium ions combined with chelating agent anions (the Gibbs free energy of the lithium ions reaction was more negative). As interaction analysis result showed, the Li-O bond in complex interaction was significantly stronger than the Li-O bond interaction in aqueous phase, which was the theroy that led to the enrichment of 7 Li in organic phase. In the activated carbon treatment experiment, it was found that part of lithium chelator solved in aqueous phase, which resulted in that the lithium abundance of aqueous phase hardly changed. In order to increase the abundance of 6 Li in aqueous phase, it is necessary to add adjuvant to reduce the water solubility of lithium chelate. Overall, an economical and environmental-friendly precipitation method with a certain application prospect for lithium isotope separation was proposed, and the direction of improving the separation performance was pointed out in this paper.
✓ 已验证
产品关联
DOI
高影响因子
DIGESTIVE DISEASES AND SCIENCES
·
2023
·
IF 3.1
Tian Xiaorong,Wang Yuxin,Yuan Mengshu,Zheng Weiping,Zuo Huaiwen,Zhang Xinru,Song Hongli
Background Ischemia–reperfusion injury (IRI) is an important cause of graft dysfunction post-liver transplantation, where donor liver with severe steatosis is more sensitive to IRI. Liver IRI involves ferroptosis and can be alleviated by heme oxygenase-1-modified bone marrow mesenchymal stem cells (HO-1/BMMSCs). Aims To explore the role and mechanism of HO-1/BMMSCs in severe steatotic liver IRI. Methods A severe steatotic liver IRI rat model and a hypoxia/reoxygenation (H/R) of severe steatosis hepatocyte model were established. Liver and hepatocyte damage was evaluated via liver histopathology and cell activity. Ferroptosis was evaluated through ferroptosis indexes. Nuclear factor erythroid 2-related factor 2 (Nrf2) was knocked down in severe steatotic hepatocytes. The role of Nrf2 and AMPK in HO-1/BMMSC inhibition of ferroptosis was examined using the AMP-activated protein kinase (AMPK) pathway inhibitor Compound C. Results The HO-1/BMMSCs alleviated severe steatotic liver IRI and ferroptosis. HO-1/BMMSCs promoted ferritin heavy chain 1(FTH1), Nrf2, and phosphorylated (p)-AMPK expression in the H/R severe steatotic hepatocytes. Nrf2 knockdown decreased FTH1 expression levels but did not significantly affect p-AMPK expression levels. The protective effect of HO-1/BMMSCs against H/R injury in severe steatotic hepatocytes and the inhibitory effect on ferroptosis were reduced. Compound C decreased p-AMPK, Nrf2, and FTH1 expression levels, weakened the HO-1/BMMSC protective effect against severe steatotic liver IRI and H/R-injured severe steatotic hepatocytes, and reduced the inhibition of ferroptosis. Conclusions Ferroptosis was involved in HO-1/BMMSC reduction of severe steatotic liver IRI. HO-1/BMMSCs protected against severe steatotic liver IRI by inhibiting ferroptosis through the AMPK–Nrf2–FTH1 pathway. Graphical Abstract HO-1/BMMSCs activate AMPK, which activates Nrf2, promotes its nuclear transcription, then promotes the expression of its downstream protein FTH1, thereby inhibiting ferroptosis and attenuating severe steatotic liver IRI in rats. Glu: glutamic acid; Cys: cystine; GSH: glutathione; GPX4: glutathione peroxidase 4; HO-1/BMMSCs: HO-1-modified BMMSCs; Fer-1: ferrostatin-1; DFO: deferoxamine; FTH1: ferritin heavy chain1; p-AMPK: phosphorylated AMP-activated protein kinase; Nrf2: nuclear factor erythroid 2-related factor 2; IRI: ischemia-reperfusion injury; MCD: methionine-choline deficiency
✓ 已验证
产品关联
DOI
PubMed
高影响因子
DNA AND CELL BIOLOGY
·
2023
·Vol. 42
·期 9
·554-562
·
IF 3.1
Liangliang Yang,Yang Gao,Jinlong Huang,Hantao Yang,Puyuan Zhao,Chen Li,Zhigang Yang
Inhibition of the inflammatory response triggered by microglial pyroptosis inflammatory activation may be one of the effective ways to alleviate cerebral ischemia–reperfusion injury, the specific mechanism of which remains unclear. In this study, BV-2 microglia with or without oxygen–glucose deprivation/reoxygenation (OGD/R) or long noncoding RNA (lncRNA) Gm44206 knockdown were used as cell models to conduct an in vitro study. Detection of lactate dehydrogenase release and pyroptosis-related protein levels was performed using a corresponding kit and western blotting, respectively. Proliferation of microglia was evaluated by CCK8 assay. Enzyme-linked immunosorbent assay was applied for measuring levels of proinflammatory cytokines. This study verified the involvement of microglial pyroptosis as well as upregulation of NLRP3, Caspase-1, GSDMD, and Apoptosis-associated Speck-like protein containing a C-terminal caspase-recruitment domain (ASC) in cerebral ischemia–reperfusion injury. Moreover, knockdown of lncRNA Gm44206 could alleviate OGD/R-induced microglial pyroptosis and cell proliferation inhibition through the NLRP3/Caspase-1/GSDMD pathway, thus decreasing the release of proinflammatory cytokines, including interleukin (IL)-1β, IL-6, IL-18, and tumor necrosis factor-alpha. In conclusion, this study established a correlation between microglial pyroptosis and cerebral ischemia–reperfusion injury and identified lncRNA Gm44206 as a potential regulator of NLRP3/Caspase-1/GSDMD axis-mediated microglial pyroptosis, which could be considered a promising therapeutic target.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）