MACROMOLECULAR BIOSCIENCE
·
2017
·Vol. 17
·期 7
·1600444
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IF 3.238
Chengfeng Zhang,Ziyuan Liu,Haiye Wang,Xiaofeng Feng,Chunju He
A unique l -cysteine conjugated antifouling amphiphilic conetwork (APCN) is synthesized through end-crosslinking of well-defined triblock copolymers poly(allyl methacrylate)- b -poly(ethylene glycol)- b -poly(allyl methacrylate) via a combination of reversible addition-fragmentation chain transfer (RAFT) polymerization and thiol–ene “click” chemistry. The synthesized poly(ethylene glycol) macro-RAFT agent initiates the polymerization of allyl methacrylate in a controlled manner. The vinyl pendant groups of the precursor partially conjugate with l -cysteine and the rest fully crosslink with mercaptopropyl-containing siloxane via thiol–ene click chemistry under UV irradiation into APCNs, which show distinguished properties, that is, excellent biocompatibility, more than 39.6% water content, 101 barrers oxygen permeability, optimized mechanical properties, and more than 93% visible light transmittance. What's more, the resultant APCNs exhibit eminent resistance to protein adsorption, where the bovine serum albumin and lysozyme adsorption are decreased to 12 and 21 µg cm −2 , respectively. The outstanding properties of APCNs depend on the RAFT controlled method, which precisely designs the hydrophilic/hydrophobic segments and eventually greatly improves the crosslinking efficiency and homogeneity. Meantime, the l -cysteine monolayer can effectively reduce the surface hydrophobicity and prevent protein adsorption, which exhibits the viability for antifouling surface over and under ophthalmic devices, suggesting a promising soft contact lens.
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International Journal of Nanomedicine
·
2023
·
IF 8
Wei Nie,Yiming Gao,David James McCoul,Gregory James Gillispie,YanZhong Zhang,Li Liang,ChuangLong He
Introduction: Hierarchical nanofibrous scaffolds are emerging as a promising bone repair material due to their high cell adhesion activity and nutrient permeability. However, the existing method for hierarchical nanofibrous scaffolds fabrication is complicated and not perfectly suitable for further biomedical application in view of both structure and function. In this study, we constructed a hierarchical nanofibrous poly (l-lactic acid)/poly(ε-caprolactone) (PLLA/PCL) scaffold and further evaluated its bone healing ability.Methods: The hierarchical PLLA/PCL nanofibrous scaffold (PLLA/PCL) was prepared by one-pot TIPS and then rapidly mineralized at room temperature by an electrochemical deposition technique. After electrode-positioning at 2 V for 2 hrs, a scaffold coated with hydroxyapatite (M-PLLA/PCL) could be obtained.Results: The pore size of the M-PLLA/PCL scaffold was hierarchically distributed so as to match the biophysical structure for osteoblast growth. The M-PLLA/PCL scaffold showed better cell proliferation and osteogenesis activity compared to the PLLA/PCL scaffold. Further in vivo bone repair studies indicated that the M-PLLA/PCL scaffold could accelerate defect healing in 12 weeks.Conclusion: The results of this study implied that the as-prepared hydroxyapatite coated hierarchical PLLA/PCL nanofibrous scaffolds could be developed as a promising material for efficient bone tissue repair after carefully tuning the TIPS and electrodeposition parameters.
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Soft Matter
·
2015
·Vol. 11
·期 38
·7502-7506
·
IF 4.029
Yong Shen,Guannan Li,Yinan Ma,Deyang Yu,Jing Sun,Zhibo Li
Two novel thermo-responsive polymer brushes were prepared from L-alanine derivatives using the surface-initiated atom transfer radical polymerization (SI-ATRP) technique. The temperature-induced cell capture and release on both polymer brush modified substrates were further explored.
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BIOMATERIALS
·
2014
·
IF 8.312
Dong Ma,Qian-Ming Lin,Li-Ming Zhang,Yuan-Yuan Liang,Wei Xue
The co-delivery of drug and gene has become the primary strategy in cancer and other disease therapy. To co-deliver hydrophobic drug and functional gene efficiently into tumor cells, a star-shaped copolymer (PP-PLLD-Arg) with a photochemical internalization effect consisting of a porphyrin (PP) core and arginine-functionalized poly( l -lysine) dendron (PLLD-Arg) arms has been designed, and used to co-deliver docetaxel (DOC) and MMP-9 shRNA plasmid for nasopharyngeal cancer therapy. It was found that PP-PLLD-Arg/MMP-9 nanocomplex showed the photo-enhanced gene transfection efficiency in vitro , and could mediate a significant reduce of MMP-9 protein expression in HNE-1 cells. For co-delivery analysis, the obtained PP-PLLD-Arg/DOC/MMP-9 complexes could induce a more significant apoptosis than DOC or MMP-9 used only, and decreased invasive capacity of HNE-1 cells. Moreover, the star-shaped copolymer exhibited better blood compatibility and lower cytotoxicity compared to PEI-25k in the hemolysis and MTT assays , and also showed a good biocompatibility in vivo . Therefore, PP-PLLD-Arg with suited irradiation is a promising non-toxic and photo-inducible effective drug and gene delivery strategy, which should be encouraged in tumor therapy.
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JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2023
·
IF 6.1
Jin Chen,Yanru Zhang,Feifei Liu,Jianle Chen,Yujing Sun,Xingqian Ye,Donghong Liu,Huan Cheng
This study was to investigate the effects of different nonthermal treatments on quality attributes, anthocyanin profiles, and gene expressions related to anthocyanin biosynthesis during low-temperature storage, including pulsed light (PL), magnetic energy (ME), and ultrasound (US). Among these treatments, 1 min US treatment was the most effective method for improving fruit quality and increasing total anthocyanin contents (by 29.89 ± 3.32%) as well as individual anthocyanins during low-temperature storage of 28 days. This treatment resulted in high color intensity, intact cellular architectures, and positive sensory evaluation. In contrast, PL and ME treatments displayed negative effects on quality improvement, leading to the destruction of cell architectures and inhibiting anthocyanin levels. Furthermore, qPCR analysis revealed that the structural genes (C4H, CHS1, CHS2, CHI, F3H, ANS, and GST) related to anthocyanin biosynthesis and transport were the target genes and upregulated in response to the cavitation effect of US treatment.
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Materials Today Bio
·
2022
·
IF 10.761
Jiao Liu,Tao Li,Hao Zhang,Wenwen Zhao,Lijun Qu,Shaojuan Chen,Shaohua Wu
Bio-textiles have aroused attractive attentions in tissue engineering and regenerative medicine, and developing robust, bio-absorbable, and extracellular matrix (ECM) fibril-mimicking nanofibrous textiles is urgently required for the renewal of existing microfibrous textile-based scaffolds and grafts. In this study, an integrated electrospinning system consisting of one nanoyarn-forming unit and one hot stretching unit is reported to fabricate silk fibroin (SF)/poly (L-lactic-acid) (PLLA) nanofibrous yarns (nanoyarns). The hot stretching process is demonstrated to significantly improve the fiber alignment, crystallinity, and mechanical properties of SF/PLLA nanoyarns, compared to the unstretched controls. For instance, the fiber alignment degree of hot stretched 50/50 SF/PLLA nanoyarn has increased by 25%, and the failure strength has increased by 246.5%, compared with the corresponding un-stretched control. Increasing the SF/PLLA mass ratio is found to significantly decrease the crystallinity and mechanical properties, but notably increase the degradation rate and surface hydrophilicity of SF/PLLA nanoyarns. Different SF/PLLA nanoyarns are further meticulously interwoven with warp and weft directions to obtain several nanofibrous woven textiles. The results from in vitro cell characterization and in vivo subcutaneous implantation show that increasing the SF/PLLA mass ratio significantly improves the biological properties and effectively reduces the inflammatory response of nanoyarn-constructed textiles. Overall, this study demonstrates that our SF/PLLA nanoyarns with controllable physical, mechanical and biological performances are fantastic candidates for the designing and development of advanced nanoarchitectured textile tissue scaffolds.
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XENOBIOTICA
·
2021
·
IF 1.908
Weibin Xiao,Zhirong Deng,Chongfa Lai,Haoyang Lu,Mutu Huang,Yuguan Wen,Lei Shi
l-tetrahydropalmatine (l-THP) is mainly metabolised by CYP450 enzymes.This study was to investigate the possible effect of co-administered CYP inhibitors on the pharmacokinetics of l-THP and its metabolites in rats.An established LC-MS/MS method has been applied for the evaluation of drug-drug interaction between l-THP and CYP inhibitors. Following the administration of CYP inhibitors, a single dose of l-THP (9 mg/kg) was orally administrated.With regard to l-THP, the AUC0–48 were significantly increased by 4.3, 3.79, and 11.39 folds, and Cmax were increased by 4.74, 3.64, and 2.76 folds in the ketoconazole group (KET), quinidine group (QD), and 1-aminobenzotriazole group (ABT), respectively. KET and QD both significantly increased the AUC0–48 of 2-DM and 2-DM-Glu by 1.38 ∼ 2.43 times, while Cmax was significantly decreased by 41.3 and 78.0% in the ABT group, respectively. The Cmax of 3-DM was reduced by 51.38, 48.02, and 63.31% after pre-treatment with KET, QD, and ABT, respectively, and Cmax of 3-DM-Glu decreased correspondingly by 29.6, 22.1, and 58.0%.Results indicated that CYP inhibitors could markedly influence the systemic level of l-THP and its metabolites. To guarantee the safe use of l-THP, attention should be paid when l-THP was co-administered with CYP inhibitors, particularly with CYP3A4 and 2D6 inhibitors.
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JOURNAL OF THE ELECTROCHEMICAL SOCIETY
·
2022
·Vol. 169
·期 7
·077508
·
IF 4.386
Chi Zhang,Yanli Ji,Yulong Zhang,Baoxian Ye,Lina Zou
In this work, a green and low-cost voltammetric sensor, poly(L-Ornithine) film modified glassy carbon electrode (PLO/GCE) was fabricated for the first time to detect thiamethoxam. The PLO/GCE was prepared by one-step electrodeposition, which is environmentally friendly, convenient and highly controllable. The modified electrode was characterized by electrochemical method, IR and SEM. Moreover, the redox character of thiamethoxam at proposed sensor was studied systematically. The PLO/GCE showed high sensitivity to thiamethoxam detection. Utilizing the linear sweep voltammetry, a wider linear range (5.0 × 10−8 mol l−1 −9.0 × 10−6 mol l−1) and lower detection limit (1.7 × 10−8 mol l−1) (S/N = 3) were achieved. In addition, the proposed method was successfully applied to the determination of thiamethoxam in pesticide.
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Agronomy-Basel
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2022
·Vol. 13
·期 1
·121
·
IF 3.949
Xiaoyun Huang,Hongxing Chen,Hui Chen,Chengwu Fan,Yueying Tai,Xiaoran Chen,Wang Zhang,Tengbing He,Zhenran Gao
The spatiotemporal heterogeneity of rice canopy leaves at different leaf positions is very important for non-destructive monitoring under Cadmium (Cd) stress, and is one of the key problems that need to be solved urgently in modern agriculture. This study aims to explore the temporal and spatial heterogeneity of chlorophyll content and fluorescence in rice canopy leaves under different Cd stress concentrations. The responses of the relative chlorophyll content (SPAD) and Chlorophyll fluorescence (ChlF) parameters to Cd stress in the tillering, heading, and filling stages were analyzed through pot experiments with three Cd concentrations: 0.20 mg·kg−1(CK), 0.60 mg·kg−1(Cd1), and 1.60 mg·kg−1(Cd2). Results showed that the canopy leaf SPAD value increased with increasing Cd concentration. Compared with CK, the leaf SPAD value in the Cd1 and Cd2 treatments were 1.91–3.45% and 5.52–12.01% lower, respectively. The SPAD value of the lower leaves was higher in the tillering stage, while the SPAD value of the upper leaves was higher in the heading and filling stages. The non-photochemical quenching coefficient (NPQ) of the third leaf (D3), the initial fluorescence yield (Fo) of the second leaf (D2), and the maximum photochemical efficiency (Fv/Fm) of photosystem II (PSII) in the tillering stage; the NPQ, Fo, and Fv/Fm of the D3 in the heading stage; and the NPQ, Fo, Fv/Fm, and Fv/Fm of the D2 in the filling stage were more sensitive to Cd stress than the other leaves and ChlF parameters.Keywords:cadmium;rice;relative chlorophyll content;chlorophyll fluorescence;photosynthesis
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CHINESE JOURNAL OF CHEMICAL ENGINEERING
·
2020
·
IF 2.627
Shanshan Pei,Xiaobo Ruan,Jia Liu,Wei Song,Xiulai Chen,Qiuling Luo,Liming Liu,Jing Wu
l -Amino acid deaminase (LAAD) is a key enzyme in the deamination of l -valine ( l -val) to produce α-ketoisovalerate (KIV). However, the product inhibition of LAAD is a major hindrance to industrial KIV production. In the present study, a combination strategy of modification of flexible loop regions around the product binding site and the avoidance of dramatic change of main-chain dynamics was reported to reduce the product inhibition. The four mutant PM-LAAD M4 (PM-LAAD S98A/T105A/S106A/L341A ) achieved a 6.2-fold higher catalytic efficiency and an almost 6.7-fold reduction in product inhibition than the wild-type enzyme. Docking experiments suggested that weakened interactions between the product and enzyme, and the flexibility of the “lid” structure relieved LAAD product inhibition. Finally, the whole-cell biocatalyst PM-LAAD M4 has been applied to KIV production, the titer and conversion rate of KIV from l -val were 98.5 g·L −1 and 99.2% at a 3-L scale, respectively. These results demonstrate that the newly engineered catalyst can significantly reduce the product inhibition, that making KIV a prospective product by bioconversion method, and also provide the understanding of the mechanism of the relieved product inhibition of PM-LAAD.
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Catalysts
·
2022
·Vol. 12
·期 9
·971
·
IF 4.501
Zewang Guo,Denghui Chen,Qi Xiong,Miao Liang,Pengfei Li,Zehui Gong,Junzhi Qiu,Liaoyuan Zhang
Leucine dehydrogenase (LeuDH) has emerged as the most promising biocatalyst for L-tert-leucine (L-Tle) production via asymmetric reduction in trimethylpyruvate (TMP). In this study, a new LeuDH namedPbLeuDH from marinePseudomonas balearicawas heterologously over-expressed inEscherichia coli, followed by purification and characterization.PbLeuDH possessed a broad substrate scope, displaying activities toward numerous L-amino acids andα-keto acids. Notably, compared with those reported LeuDHs,PbLeuDH exhibited excellent catalytic efficiency for TMP with aKmvalue of 4.92 mM and akcat/Kmvalue of 24.49 s−1mM−1. Subsequently, L-Tle efficient production was implemented from TMP by whole-cell biocatalysis using recombinantE. colias a catalyst, which co-expressedPbLeuDH and glucose dehydrogenase (GDH). Ultimately, using a fed-batch feeding strategy, 273 mM (35.8 g L−1) L-Tle was achieved with a 96.1% yield and 2.39 g L−1h−1productivity. In summary, our research provides a competitive biocatalyst for L-Tle green biosynthesis and lays a solid foundation for the realization of large-scale L-Tle industrial production.Keywords:leucine dehydrogenase;L-tert-leucine;trimethylpyruvate;reductive amination;whole-cell biocatalysis
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JOURNAL OF BIOACTIVE AND COMPATIBLE POLYMERS
·
2015
·
IF 2.352
Yuan Yao,Haifeng Liu,Xili Ding,Xiaohui Jing,Xianghui Gong,Gang Zhou,Yubo Fan
In recent years, silk fibroin has become one of the most promising tissue engineering materials because of its excellent cytocompatibility. Poly(l-lactide-co-ε-caprolactone), the copolymer of poly(l-lactide) and poly(ε-caprolactone), possesses good mechanical properties, and its degradation rates can be manipulated by varying the ratio of the constituent polymers. In this study, in order to combine their respective characteristics, silk fibroin/poly(l-lactide-co-ε-caprolactone) nanofibrous membranes were fabricated through electrospinning with different mass ratios of 100:0, 75:25, 50:50, 25:75, and 0:100. The surface properties, thermodynamic properties, mechanical properties, and cytocompatibility of silk fibroin/poly(l-lactide-co-ε-caprolactone) blended membranes were evaluated, and an optimal blending ratio was identified. The results showed that the silk fibroin/poly(l-lactide-co-ε-caprolactone) blended membranes containing 75% of silk fibroin and 25% of poly(l-lactide-co-ε-caprolactone) achieved the most improved performances compared with the single-component membranes or the blended membranes with other mixing ratios. The results from this study indicated that 75/25 silk fibroin/poly(l-lactide-co-ε-caprolactone) blended membranes which combined the advantages of poly(l-lactide-co-ε-caprolactone) and silk fibroin might be a suitable candidate material for use in tissue engineering.
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