SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
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2022
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IF 4.831
Xiaoyuan Ma,Shan Xu,Liangyu Li,Zhouping Wang
Here, an aptamer-based SERS method for the detection of Staphylococcus aureus ( S. aureus ) without immobilization using [email protected] NPs/slide as enhanced substrate was constructed. ROX-aptamer of S. aureus was modified on the surface of [email protected] NPs/slide through electrostatic interaction. Based on the specific binding effect of the aptamer to S. aureus , the ROX-aptamer fell off from the surface of the substrate, resulting in a decrease of the SERS signal intensity of the substrate. Under the optimal experimental conditions, a good linear relationship was found between SERS intensity at 1500 cm −1 and the logarithm of concentration of S. aureus in the range of 10 2 cfu/mL-10 7 cfu/mL (y = 6623-796lgx, R 2 = 0.994) with a detection limit of 6 cfu/mL. The selectivity analysis revealed that the method had higher selectivity toward the corresponding target. The results for milk sample using the developed SERS method for the detection of S. aureus were similar to those of the plate counting method. The recovery ratio was from 90.60% to 107.26%, indicating the accuracy and reliability of the developed method. This method eliminates the need for bacterial immobilization and improves the convenience and efficiency of detection.
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Nanoscale
·
2022
·Vol. 14
·期 21
·7807-7816
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IF 8.307
Hongyu Du,Yunqing Wang,Panpan Zhang,Rongchao Mei,Yunxia Ji,Xizhen Zhao,Zhiyang Zhang,Jiping Ma,Lingxin Chen
Nanoplastics (NPs) as emerging marine pollutants can be taken up by seafood organisms. It is crucial to quantitatively assess NP's distribution behavior in organisms to elucidate concentration dependent biological effects. Such a knowledge gap has remained due to the lack of reliable NP models and analytical methods. Herein, surface enhanced Raman scattering (SERS)-labeled NP models were developed and their bioavailability, distribution and accumulation in Ruditapes philippinarum, a typical marine bivalve, were quantitatively studied. Taking advantage of the sensitive and characteristic SERS signals of the NP models, distribution could be quickly and accurately obtained by the Raman imaging technique. Moreover, quantitative analysis of NPs could be performed by the detection of gold element contents via inductively coupled plasma mass spectroscopy (ICP-MS) detection. ICP-MS results revealed that after 3 days exposure of monodispersed NPs (100 nm, 0.2 mg L−1), the digestive gland accumulated 86.7% of whole-body NPs followed by gill (5.2%), mantle (5.1%), foot (1.3%), exhalant siphon (1.1%), and adductor (0.6%). Upon 11 days depuration, 98.7% of NPs in the digestive gland were excreted, whereas the clearance ratios in other organs were much lower. NP aggregates (around 1.5 μm) demonstrated similar distribution and clearance trends to the monodispersed ones. However, the accumulation amount in each organ was 15.2% to 77.6% lower. Surface adherence and passive ingestion routes resulted in NP accumulation, which contributed to the comparable NP abundance in these organs. Additionally, boiling treatment (mimicking a cooking process) did not decrease the NP amount in these organs. This work provided a dual-mode and quantitative analysis protocol for NPs for the first time, and suggested the risk of NP uptake by humans via bivalve seafood diets.
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JOURNAL OF FOOD COMPOSITION AND ANALYSIS
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2022
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IF 4.52
Alberta Osei Barimah,Ping Chen,Limei Yin,Hesham R. El-Seedi,Xiaobo Zou,Zhiming Guo
Detrimental health effects caused by the intake of food contaminated with heavy metals have drawn concerns on effective monitoring using rapid and benign methods. This work presented a novel surface-enhanced Raman scattering (SERS)-based 3-Aminobenzeneboronic acid (ABBA) labeled silver (Ag) nanosensor combined with chemometric algorithms to detect and predict total arsenic (TAs) in acid digested spiked black tea leaves. The sensor recognizes TAs through the partial detachment of ABBA and the chemical formation of As-O-Ag linkage between the TAs and the Ag nanoparticles , which caused a SERS-on signal enhancement effect. SERS combined with competitive adaptive reweighted sampling partial least squares algorithm predicted the TAs with higher correlation coefficient (R p ) results (R p = 0.9750) and a detection limit of 0.0273 µg/g. Good recoveries of 83.84–109.53% and the excellent agreement with the inductively coupled plasma-mass spectrometry method (R 2 = 0.999) revealed this developed rapid method could be deployed for fast-tracking of As in food samples.
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SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
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2022
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IF 4.831
Lijun Yin,Min Fan,Qiutian She,Ruiyun You,Yudong Lu,Dechan Lu,Minlin Li
Tetrodotoxin (TTX) is one of the most lethal neurotoxins, so the reliable quantitative analysis of TTX is crucial for food and environmental safety monitoring. Herein, a novel dual-molecule calibration aptasensor was developed for detection of TTX based on Surface-enhanced Raman scattering (SERS). The adaptive surface has high affinity recognition sites for the target of interest, which ensures the high specificity and stability of the aptasensor. In addition, the uniquely labeled signal molecules located in the Raman silent region (1800–2400 cm −1 ) can avoid the interference of other exogenous biological signal molecules. Meanwhile, in quantitative analysis, the SERS signal generated by the reporter is calibrate in real time using the second-order peak of silicon molecule (Si). The detection linear range of the aptasensor was 0.0319 ng/mL-319.27 ng/mL, with a limit of detection (LOD) of 0.024 ng/mL and the excellent uniformity (RSD = 8.8%) for TTX detection. As a promising and versatile detection candidate, the ultra-sensitive quantitative detection aptasensor of TTX had important practical significance, which can offer more favorable persuasion for TTX analysis in real seafood samples.
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ANALYST
·
2022
·Vol. 147
·期 9
·1815-1823
·
IF 5.227
Yue Wang,Cheng Cheng,Ruofei Ma,Zhangrun Xu,Yukihiro Ozaki
Visualization of signaling molecules in single living cells is crucial for understanding cellular metabolism and physiology, which can provide valuable insights into early diagnoses and treatments of diseases. Highly sensitive in situ monitoring of intracellular analytes released from single living cells by virtue of label-free nanosensors is urgently needed, which can avoid interferences from molecular labeling. Here, we proposed an ultrasensitive strategy for in situ imaging of intracellular H2O2 in single living cancer cells by surface-enhanced Raman scattering (SERS) spectroscopy with the utilization of label-free Fe3O4@Ag core–satellite nanoparticles (NPs). The Fe3O4@Ag NPs can efficiently and selectively catalyze the oxidation of the peroxidase substrate 3,3′,5,5′-tetramethylbenzidine (TMB) in the presence of H2O2. Additionally, they exhibit excellent SERS activity that allows for in situ monitoring of intracellular H2O2 in living cells through establishing the correlation between the H2O2 level and the SERS intensity of the catalytic oxidation product of TMB. The H2O2 concentration is revealed through the SERS intensity of oxidized TMB with a good linear response in a wide range from 1 fM to 1 mM. Moreover, the intracellular H2O2 level in live cancer cells and imaging of the distribution of H2O2 inside single cells can be achieved by using such a label-free nanosensor based strategy. Our work demonstrates that the label-free Fe3O4@Ag NP-based SERS imaging and quantification strategy is a promising and powerful approach to assess intracellular H2O2 in living cells and allows us to monitor single-cell signaling molecules with nanoscale resolution.
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TALANTA
·
2021
·
IF 6.057
Yanan Song,Jingyi Sun,Chunmei Li,Li Lin,Feng Gao,Mingfeng Yang,Baoliang Sun,Ying Wang
Sensitive and accurate early detection of fetal growth restriction (FGR) is of vital importance in the development of the fetus during pregnancy and even the health of future life. Here, an ultrasensitive and straightforward surface-enhanced Raman scattering (SERS)-based double targets detection in pregnancy is implemented by utilizing functionalized Ag nanoparticles (AgNPs). Through fabricating 4-MPA antibody-modified AgNPs on the lateral flow assay (LFA) strips as the SERS nanotags, the target proteins in blood samples from pregnancy were accurately captured, which further quantizing PI3K and CRAF in unprocessed blood. This strategy warrant excellent selectivity and sensitivity with the limits of detection (LODs) are 0.76 fg mL −1 for PI3K and 0.61 fg mL −1 for CRAF, leading reliable quantification for these two targets. Meanwhile, the feasibility of this assembly was testified by comparing with conventional ELISA method, and the results showed that a high degree of consistency was obtained in these two detection assays. This SERS-colorimetric dual-signal LFA strip can provide a novel strategy for early diagnosis of fetal-related disorders, which is essential for disease diagnosis and treatment guidance during pregnancy.
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JOURNAL OF COLLOID AND INTERFACE SCIENCE
·
2023
·
IF 9.9
Wei-Bin Wang,Jian-Jun Li,Guo-Jun Weng,Jian Zhu,Yu-Bo Guo,Jun-Wu Zhao
Herein, A novel gold-silver alloy nanobox (AuAgNB)@SiO 2 -gold nanosphere (AuNP) nanoassembly based on core–shell-satellite structure is fabricated and applied to the surface-enhanced Raman scattering (SERS) detection of S100 calcium-binding protein B protein (S100B). It contains an anisotropic hollow porous AuAgNB core with rough surface, an ultrathin silica interlayer labeled with reporter molecules, and AuNP satellites. The nanoassemblies were systematically optimized by tuning the reporter molecules concentration, silica layer thickness, AuAgNB size, and the size and number of AuNP satellite size. Remarkably, AuNP satellites are adjacent to [email protected] 2 , developing AuAg-SiO 2 -Au heterogeneous interface. With the strong plasmon coupling between AuAgNB and AuNP satellites, chemical enhancement from heterogeneous interface, and the tip “hot spots” of AuAgNB, the SERS activity of the nanoassemblies was multiply enhanced. Additionally, the stability of nanostructure and Raman signal was significantly improved by the silica interlayer and AuNP satellites. Eventually, the nanoassemblies were applied for S100B detection. It demonstrated satisfactory sensitivity and reproducibility with a wide detection range of 10 fg/mL-10 ng/mL and a limit of detection (LOD) of 1.7 fg/mL. This work based on the [email protected] 2 -AuNP nanoassemblies with multiple SERS enhancements and favorable stability demonstrates the promising application in stroke diagnosis.
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SENSORS AND ACTUATORS B-CHEMICAL
·
2021
·
IF 7.46
Saisai Jiao,Jie Liu,Jialong Sun,Yunwei Chang,Siwei Wang,Shijie Dai,Ruimin Xu,Menghua Dou,Qianjin Li,Jing Wang,Jianlin Li
A highly sensitive and reproducible multiplex mycotoxin SERS array has been established based on gold nanoparticles (AuNPs)-loaded inverse opal silica photonic crystal microsphere (SIPCM). Three types of Raman nanotags were synthesized by covalently grafting different antibodies and non-covalently loading different organic dyes onto the AuNPs for coding corresponding mycotoxins. To enhance Raman signal, AuNPs were loaded on the surface of SIPCM to create hot-spots once the Raman nanotags came closely. The maximal electric field enhancing effect in SIPCM with AuNP dimer could reach to 8 times. The assay principle was based on the competition immunoassay between the mycotoxins in sample and the artificial antigens immobilized on the surface of SIPCM. The three Raman nanotags could be integrated into an array to qualitatively and quantitatively detect multiplex mycotoxins in a sample. Such SERS-based assay displayed wide linear detection ranges of 0.001–10 ng/mL for ochratoxin A (OTA), 0.001–10 ng/mL for fumonisin B 1 (FB 1 ), and 0.1–1000 ng/mL for deoxynivalenol (DON), and low limits of detection (LOD) of 2.46 pg/mL for OTA, 0.20 pg/mL for FB 1 , and 68.98 pg/mL for DON. The recovery rates of the new method were measured to be 77.68–104.69% for OTA, 76.39–117.73% for FB 1 , and 70.95–113.16% for DON, in agreement with that of the classical ELISA method. The SERS-based method shows obvious advantages over fluorescence label or enzyme catalytic chemiluminescence methods , having a great application potential in rapidly screening multiplex mycotoxins in real samples.
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ANALYTICA CHIMICA ACTA
·
2023
·
IF 6.2
Yue Pan,Shan Xu,Zhouping Wang,Caiyun Jiang,Xiaoyuan Ma
Background Histamine is a kind of biogenic amine with strong toxicity and potential carcinogenicity . Many traditional methods of detecting histamine have the disadvantages of cumbersome detection steps, expensive equipment, and high professional requirements for staff. In contrast, SERS has become the preferred method for quantitative analysis of histamine because of rich fingerprint information, rapidity and economy. However, most of SERS substrates still have technical problems, such as poor stability, low sample collection rate, and detection efficiency. Therefore, there is a great need for new strategies to develop high-performance SERS substrates based sensors. Results In our study, a sensitive SERS aptasensor for the detection of histamine was synthesized. The assembly was formed between IRMOF-3@Au/PDMS (flexible SERS substrate) and AuNR-DTNB@Ag-HA apt (Raman signal probe with both the target capture ability) via π-π stacking interaction from HA aptamer and IRMOF-3. Consequently, the SERS signal of the assembly derived from DTNB reached highest due to the synergistic enhancement effect by AuNR@Ag and IRMOF-3@Au. Meanwhile, HA aptamer can specifically capture histamine, therefore histamine addition competitively bound to the probe, leading to a corresponding decrease in the DTNB signal value on the SERS substrate. The SERS intensity at 1331 cm −1 presented a good linear relationship towards the logarithmic value of histamine concentrations ranging from 0.0001 mg/L to 400 mg/L (R 2 = 0.990) with the LOD of 3.6 × 10 −5 mg/L. Furthermore, the application in wine samples demonstrated the accuracy and applicability of the developed sensor. Significance This method effectively improves substrate stability, detection sensitivity and signal response immediacy to amplify the SERS sensor, thus satisfying the histamine detection requirements of various systems. According to this aptasensor design, our strategy can be extended to create other MOF-based SERS substrates for accurately detecting relative targets to ensure food safety.
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CHEMICAL ENGINEERING JOURNAL
·
2023
·
IF 15.1
Jiansen Lie,Feili Luo,Yafang Liu,Yixuan Yang,Qingling Nie,Xiaochuan Chen,Ruiyun You,Yunzhen Liu,Xiufeng Xiao,Yudong Lu
As an important enzyme in human tissues, abnormally alkaline phosphatase (ALP) is strongly associated with many diseases, particularly breast and prostate cancer. In this study, we proposed a rapid and sensitive surface-enhanced Raman scattering (SERS)-based biosensor for ALP detection. The biosensor utilized Fe 3 O 4 @Au@TiO 2 nanoparticles (FAT NPs) as magnetic separation and enrichment materials to enrich grafted mediator Ag-4-mercaptophenylboronic acid (4-MPBA)-ascorbic acid 2-phosphate (AAP) NPs to form FAT-(AAP-4-MPBA-Ag) NPs, and 5,5′-dithiobis-(2-nitrobenzoic acid) (DTNB) was used as the internal standard tag molecule to prepare Au@DTNB@Ag as the signal source. The phosphate group in AAP was cleaved by ALP to form ascorbic acid (AA), which acts as a receptor for the boric acid to adsorb the 4-MPBA-Au@DTNB@Ag NPs. When the switch was “Off” to “On”, the Au@DTNB@Ag NPs were used to significantly improve the sensitivity of the proposed SERS platform, enabling detection as low as 1.17U/L of target ALP with a good linear range (R 2 = 0.991) of 2–10 U/L. Furthermore, the platform successfully detected ALP from 2 % human serum. The recoveries were 99.5–106 %. In addition, FAT-(AAP-4-MPBA-Ag) NPs could be transformed into FAT NPs to achieve recyclability by ultrasonication in acidic environments. Overall, this sensitive and fast SERS platform has promising potential for accurate and efficient identification of ALP from human serum.
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Surfaces and Interfaces
·
2023
·
IF 6.2
Jiayao Zhang,Danni Xue,Huan Liu,Zhao Wei,Chenjie Gu,Shuwen Zeng,Junhui Jiang,Tao Jiang,Xingfei Zhou,Kerong Wu
Attributed to their structure-dependent chemical enhancement capability and perfect biocompatibility, semiconductors have attracted significant concern in surface enhanced Raman scattering (SERS)-based immunoassay. By continually adjusting the size of red phosphorus (RP) in the transition process from bulk to nanosheet, we investigated in detail about the improvement of high-efficient charge transfer (CT) resonance dependent on the perfect energy level matching within the substrate-molecule combination. The nano-sized RP has achieved an impressive three-fold enhancement in SERS intensity in comparison to the RP nanosheet. Furthermore, the RP-based immunosubstrate was combined with MoS 2 nanoflowers-based immunoprobe to construct a sandwich immunostructure for the accurate and efficient diagnostics of cancer marker prostate-specific antigen. A limit of detection as low as 3.99 × 10 −10 M was obtained in the SERS-based immunoassay with reliable specificity. This study presents an innovative approach to promote the SERS performance of semiconductors, which may find potential biological applications in the early screening of cancer.
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ANALYTICAL CHEMISTRY
·
2023
·
IF 7.4
Dan Sun,Guohua Qi,Xuan Yi,Hongyan Zhu,Yongdong Jin
Studying the oxidative stress, especially the reactive oxygen species (ROS) response of ferroptosis, is crucial for the diagnosis and treatment of cancer based on ferroptosis. However, reliable quantitative analysis of intracellular ROS in cancer treatment for drug screening is still a challenge. Herein, a superior ratiometric SERS nanoprobe was developed for in situ, real-time, and highly sensitive detection of content variation of H2O2 within living cells. The SERS nanoprobe was prepared by coassembly of the internal standard molecule p-mercaptobenzonitrile and the reporter molecule p-mercaptophenylboronic acid on the surface of gold nanoparticles, used for synergistic calibration and detection of H2O2, which enables reliable detection of the true content of intracellular H2O2 without the interference of other substances in cells. Based on the nanoprobe, we found that the level of intracellular H2O2 of cancer cells was increased after the nicotinamide adenine dinucleotide (NADH) treatment, with a dose-dependence to the concentration of NADH. High doses of NADH (above 20 mM) can induce cell death by means of ferroptosis associated with the level elevation of intracellular lipid hydroperoxides. This study highlights the potential of the SERS nanoprobe for tracking content variation of cellular H2O2 and understanding its roles in screening new anticancer drugs.
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