Chinese Medicine
·
2025
·Vol. 20
·期 1
·1-20
·
IF 5.7
Jin Chengbo,Ma Tianle,Zhang Yiheng,Gu Xujing,Zhu Tong,Wu Xinyu,Ding Xin,Huang Suzhou,Wang Yulan,Chen Zhipeng,Fang Huihua,Wu Li
Background The progression of liver cirrhosis leads to severe complications, significantly threatening the survival and prognosis of patients. Rou gan keli (Rgkl), a herbal formula derived from classical prescriptions, has used clinically over two decades and has good efficacy. However, its molecular mechanisms and active components remain undefined. Purpose Exploring the molecular mechanisms of Rgkl in alleviating liver cirrhosis. Methods CCl 4 -induced liver cirrhosis mice models were established. Liver stiffness and intrahepatic blood flow velocity were assessed using imaging. Serum ALT, AST, HA, and histopathology were analyzed. Hepatic stellate cells (HSCs) activation, liver sinusoidal endothelial cells (LSECs) fenestration, and angiogenesis were evaluated using immunohistochemistry and scanning electron microscopy. UPLC-Q-TOF-MS/MS and network pharmacology identified active components. Transcriptomics and single-cell sequencing identified key targets and pathways, validated via WB, immunofluorescence, and molecular docking. Results Rgkl significantly reduced Liver stiffness and collagen deposition while increasing intrahepatic blood flow velocity in cirrhotic mice. Serum ALT, AST, and HA were markedly decreased. Rgkl inhibited α-SMA expression in HSC and downregulated pathological angiogenesis by reducing VEGF and CD34 expression. Additionally, Rgkl enhanced eNOS expression and preserved sinusoidal fenestration in LSEC. Furthermore, Rgkl ameliorated liver cirrhosis by modulating LSEC metabolic functions via the CD36/PPAR/CPT-1 pathway and suppressing HSC activation through the RhoA/ROCK/YAP and PI3K/AKT/NF-κB pathways. Eighteen active components, such as Levistilide A and Quercetin, were strongly correlated with the amelioration of liver cirrhosis. Conclusions Rgkl significantly attenuated hepatic injury and fibrosis. Mechanistically, Rgkl modulated LSEC lipid metabolism and phenotypic regulation, and suppressed HSC contraction and activation. Key active components contributing to these effects included Paeonilactone C, Levistilide A, and Quercetin. Graphical
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MICROCHIMICA ACTA
·
2025
·Vol. 192
·期 10
·1-11
·
IF 5.3
Wang Liu,Wang Mingli
A selective detection strategy is proposed for the determination of residual hypochlorite in drinking water. Flower-like Fe 3 O 4 @SiO 2 @Ag magnetic nanoparticles can aggregate on a silver film-coated magnetic substrate surface under a magnetic field, enabling the separation and enrichment of the analyte from the sample. 4-Mercaptophenol is functionalized on magnetic nanoparticles enabling quantitative detection of ClO − through a “signal off” mechanism. This substrate exhibits high sensitivity, with an analytical enhancement factor calculated using Rhodamine B (RhB) as an example reaching 1.89 × 10 5 , and a detection limit for ClO − as low as 0.0125 mg/L. The substrate also demonstrates good uniformity, reproducibility, and selectivity. Detection in actual drinking water samples indicates the potential application of this detection strategy in food safety. Graphical
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JOURNAL OF SOLID STATE ELECTROCHEMISTRY
·
2025
·
IF 2.6
Cao Bo-Qun,Bao Hao-Tian,Zhang Ji-Wei,Shao Gang-Qin
The lithium-phosphorus-thiohalides (LPS X ) with superionic conduction are strong candidates for the new-generation solid electrolytes (SEs) in all-solid-state lithium-ion batteries (ASSLIBs). In this work, Li 6− x PS 5− x Cl 1+ x − y Br y (LPSCB; 0 ≤ x ≤ 0.7; 0 ≤ y ≤ 1.7) was synthesized by a solid-state method in which the key raw material of Li 2 S was prepared by an improved carbothermal reduction method without late decarbonization. The lattice flexibility and the degree of structural order are opposite effect and would lead to an optimum of the ionic conductivity at a composition of LPSCB. Li 6 PS 5 Br- and Li 5.5 PS 4.5 Br 1.5 -based ASSLIBs had the best rate capacity and cycle performance in Li 6 PS 5 Cl 1− y Br y - (0 ≤ y ≤ 1) and Li 5.5 PS 4.5 Cl 1.5− y Br y -based ones (0 ≤ y ≤ 1.5), respectively. To increase the ionic conductivity of LPSCB-type SEs and capacity/cycle performance of the related ASSLIBs, Br-introduction, mutual substitution and excess- X substitution were extremely effective strategies. Graphical
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ANALYTICAL AND BIOANALYTICAL CHEMISTRY
·
2018
·Vol. 410
·期 24
·6197-6205
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IF 3.307
Xie Sanlei,Wen Kai,Xie Jie,Zheng Yongjun,Peng Tao,Wang Jianyi,Yao Kai,Ding Shuangyang,Jiang Haiyang
A sensitive competitive immunoassay with simple operation was developed for the detection of the anti-virus drug amantadine (AMD). The single-chain variable fragment (scFv) antibody against AMD was site-specific biotinylated and overexpressed as a secreted body in Escherichia coli AVB101. Horseradish peroxidase-labeled streptavidin-biotinylated scFv antibody (HRP-SA-BIO-scFv) could specifically bind to AMD-functionalized magnetic beads (MBs) and then the immune complexes were separated from the matrix solution by magnet. The concentration of the AMD could be known by the measurement of the signal produced by the horseradish peroxidase. The newly established assay provides a significant improvement in comparison to the conventional ELISA without SA-BIO signal amplification and MBs separation. The limit of detection and assay time was 0.64 vs. 8.4 ng/mL and 50 vs. 150 min, respectively. The recoveries ranged from 77.8 to 112% with the coefficient of variation less than 13%. The immunoassay exhibited an obvious cross-reactivity to rimantadine (84%), 1-(1-adamantyl)ethylamine (72%), and somantadine (63%). These results demonstrated that the developed immunoassay provided a sensitive, rapid, and accurate approach for the detection of AMD in chicken by employing MBs as solid phase and SA-BIO as signal amplification. When applied in natural chicken samples, the newly established method provided results consistent with those from UPLC-MS/MS, suggesting that the proposed method could be used for rapid screening of the target of interest; the new immunoassay could also be extended to other small molecular contaminants and thus represents a universal strategy for food safety analysis.
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Chinese Medicine
·
2025
·Vol. 20
·期 1
·1-21
·
IF 5.7
Wei Ji-chang,Lin Xiao-yu,Zhao Yi-hang,Tan Xin-ru,Wang Zhi-xia,Li Yuan-yuan,Huang Xue-mei,Wang Peng-long
Background Scutellariae Radix (SR) and Coptidis Rhizoma (CR) are classic drug pairs used in clinical practice for clearing heat and drying dampness, purging fire for removing toxin. By further studying the mechanism of compatibility of SR and CR from the perspective of thermodynamically driven supramolecular phase transition, we could reveal the interaction between its pharmacodynamic components, and provide scientific basis for improving TCM efficacy. Methods The SR-CR and its main components baicalin-berberine (BA-BBR) were taken as the research objects. The morphology of the mechanically mixed samples was characterized by malvern particle size analyzer and scanning electron microscope. UHPLC-Q-Orbitrap HRMS technology was employed to analyze the material basis of each mechanically mixed sample. ITC was used to investigate the effect of temperature on the binding ability between SR and CR. The structural differences of supramolecules in different morphology were explored by molecular dynamics simulation. Finally, in vitro antibacterial models ( E. faecium and B. subtilis , S. aureus ) were used to evaluate the antibacterial activities of the mechanically mixed samples and non-targeted metabolomics was used to explore the differences in antibacterial mechanisms. Results The mechanical mixtures formed nanofibers (NFs), while heating induced a transition to nanospheres (NPs). Molecular dynamics simulations revealed that enhanced hydrogen bonding and tighter molecular packing under thermal conditions drove this morphological shift. In vitro antibacterial assays and non-targeted metabolomics showed NPs exhibited superior inhibition against Staphylococcus aureus by disrupting amino acid biosynthesis and metabolism, whereas NFs suppressed Bacillus subtilis via physical entanglement and interfered with energy metabolism. Conclusion Driven by thermal energy, the existence form of supramolecules changed from NFs to NPs and the morphology of the formed supramolecules was maintained during their interaction with bacteria, further affected the biological activity. Graphical
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CELLULAR AND MOLECULAR LIFE SCIENCES
·
2025
·Vol. 82
·期 1
·1-17
·
IF 6.2
Zhong Chong-Chao,Zhang Xu,Pantopoulos Kostas,Song Chang-Chun,Yang Hong,Wei Xiao-Lei,Luo Zhi
Copper (Cu) and zinc (Zn) are essential trace elements for terrestrial animals. Studies suggested that single dietary Cu- or Zn- deficiency lead to liver lipid deposition and cause metabolic dysfunction-associated steatotic liver disease (MASLD). However, the mechanisms by which dietary Cu and Zn co-deficiency promote hepatic lipid deposition and metabolism remain unknown. Our study found that, compared to single Cu- or Zn- deficiency, Cu and Zn co-deficiency significantly increased lipid deposition, induced mitochondrial oxidative stress, disrupted mitochondrial structure and function, and inhibited lipolysis in hepatocytes and whole liver tissue. Mechanistically, Cu and Zn co-deficiency-induced lipotoxicity was mediated by sirtuin 3 (Sirt3). Sirt3 inhibited the acetylation and degradation of forkhead box O3 (Foxo3), preventing its binding to the peroxisome proliferator-activated receptor alpha (PPARα) promoter, which accordingly increased lipid deposition. Overall, for the first time, we elucidated the mechanism by which Cu and Zn co-deficiency aggravates hepatic lipotoxicity and identified the critical regulatory role of the Sirt3/Foxo3/PPARα pathway during this process, with the fish as the model.
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npj Science of Food
·
2025
·Vol. 9
·期 1
·1-13
·
IF 7.8
Liao Yingyi,Xie Xingyu,Lin Zixin,Huang Ning,Wei Guilan,Wu Jiazhen,Li Yucui,Chen Jiannan,Su Ziren,Yu Xiuting,Chen Liping,Liu Yuhong
Visceral adiposity is an important characteristic of metabolic dysfunction-associated steatohepatitis (MASH) that affects its development. Curcumin (Cur) attenuates MASH through microbial biotransformation. However, antibiotics (Abx) do not weaken but rather enhance the resistance to weight gain of Cur on MASH rats. The resistance to weight gain mechanism of Cur is different from its anti-MASH effect, which may relate to alleviate visceral adiposity. Here we investigated the mechanism of Cur against visceral adiposity in high-fat diet-induced MASH rats. The results demonstrated that Cur and Abx reduced body, liver and visceral fat weights of MASH rats. Unlike Abx, which induces resistance to weight gain by reducing appetite, Cur mainly reduced the weight of visceral fat, especially that of perirenal fat. Intriguingly, the reduction in perirenal fat caused by Cur was attributed to its specific inhibition of gastric inhibitory polypeptide (GIP) release. The Cur-induced decrease in intestinal GIP release inhibited the activation of GIP receptors to alleviate adipogenesis and inflammation in perirenal adipose tissue. Moreover, Cur alleviated intestinal epithelium and vascular barrier disruption-mediated hypoxia to inhibit GIP release. In summary, the pharmacological effects of Cur on visceral adiposity were mainly contributed by inhibiting gut barrier disruption-mediated hypoxia to attenuate GIP release.
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JOURNAL OF APPLIED ELECTROCHEMISTRY
·
2025
·
IF 3
Liu Hanzhen,Wang Yanjie,Feng Xinyu,Ling Jing,Lin Xuanyu,Lu Zhenhang,Tang Xin
The oxygen evolution reaction (OER) constitutes a pivotal step in numerous energy conversion and storage systems. The strategic advancement of economical, efficient, and resilient catalysts for the anode OER represents one of the pressing bottlenecks hindering their scalable implementation. Experimental results demonstrated that the synergistic effect of Zr doping and the Co 2 Sb 2 O 7 pyrochlore structure increased the oxygen vacancy content and significantly enhanced the catalytic activity. Thus, the appropriate proportion of Zr (only x = 0.1) doped Co 2 Sb 2 O 7 pyrochlore structure exhibited excellent OER performance, reaching 10 mA cm −2 at an overpotential of only 210 mV in 0.5 M H 2 SO 4 and at an overpotential of only 216 mV in 1 M KOH solution. The catalyst operated stably for 60 h at 1 mA cm −2 in 0.5 M H 2 SO 4 and also operated stably for 60 h at 10 mA cm −2 in 1 M KOH. This study offers a promising outlook for reducing the energy consumption of noble metal catalysts used in acidic OER processes in practical electrochemical industries. Graphical abstract
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MEDICAL ONCOLOGY
·
2025
·Vol. 42
·期 7
·1-12
·
IF 3.5
Tang Qing,Huang Kun,Li Mengjie,Zhao Ziqiao,Deng Yuanle,Zuo Yueqi,Wang Ningyu,Zhu Yongxia
Ovarian cancer is a deadly gynecological malignancy often diagnosed at an advanced stage, with few treatment options available. Polyphyllin I (PPI), the primary active ingredient of Paris polyphylla , has shown excellent anti-cancer activity against various cancers. However, studies on PPI use and ovarian cancer are limited, and the potential mechanisms remain unclear. This study explored the effects of anti-ovarian cancer and the potential mechanisms of action of PPI. Methods such as MTT, colony formation, three-dimensional spheroid formation, flow cytometry, and western blotting were employed to assess the anti-proliferative effects of PPI on different ovarian cancer cell lines. RNA sequencing was used to discover potential mechanisms by evaluating the effects of PPI on transcriptional signatures and identifying differences in gene expression patterns. The results indicate that PPI demonstrated dose- and time-dependent anti-proliferative effects on ovarian cancer cell lines. PPI also up-regulated 1518 genes and down-regulated 800 genes. The genes showed enrichment in the “apoptosis” gene sets, while exhibiting negative enrichment in the “cell cycle” and “Myc signaling pathway” gene sets. PPI caused cell cycle arrest at the G0/G1 phase by increasing the expression of p21 and p27 and inhibiting the activity of the CDK2/cyclin E complex. PPI triggered cell apoptosis, which was associated with elevated cellular reactive oxygen species and mitochondrial membrane potential depolarization. Finally, PPI reduced c-Myc protein levels and affected mRNA levels of c-Myc pathway-related genes. PPI effectively suppressed ovarian cancer cell proliferation, indicating its potential as a treatment option for ovarian cancer.
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ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
·
2024
·Vol. 31
·期 32
·44759-44769
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IF 0
Wu Rui,Song Xi,Tian Guanghui
Highly active Fe 3 O 4 /GO/Au composite nanomaterial was fabricated as a substrate of surface-enhanced Raman spectroscopy (SERS) and applied for pesticide residue detection. The three-layer multifunctional Fe 3 O 4 /GO/Au nanoparticles (NPs) were designed by facile method, with high hotspots, and were characterized by various techniques, including ultraviolet spectrophotometry (UV), X-ray diffraction (XRD), infrared absorption spectrometer (IR), and transmission electron microscopy (TEM). The performance of Fe 3 O 4 /GO/Au was evaluated by Raman spectroscopy with R6G as a probe molecule to verify its enhancement effect. It exhibited a strong Raman signal with 10 −6 M of R6G. Furthermore, the presence of Fe 3 O 4 /GO/Au nanohybrid enabled the SERS-based method to detect mancozeb and showed an excellent linear relationship in the range of 0.25–25 ppm, with a low limit of detection (0.077 ppm), satisfactory EF, stability, and repeatability. In addition, the mechanism of SERS enhancement with electromagnetic mechanism (EM) and chemical mechanism (CM) was discussed in detail. Therefore, the proposed SERS approach holds promise as an auxiliary technique for screening contaminated agricultural products, environmental sample, and food in the future.
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CHINESE JOURNAL OF POLYMER SCIENCE
·
2026
·
IF 4.6
Du Hai-Yan,Zhang Jing,Xu Qing,Cao Yi-Chang,Jia Hui,Li Ying
Gel-based flexible wearable sensors have attracted considerable interest in aquatic environments. However, the development of underwater conductive gel sensors with outstanding anti-swelling, mechanical, and sensing capabilities faces significant challenges. The aim of this study is to develop anti-swelling and conductive zwitterionic gels and investigate their applications in wireless underwater strain sensing. Multi-functional zwitterionic gels were fabricated by copolymerizing [2-(methacryloyloxy) ethyl] dimethyl-(3-sulfopropyl) ammonium hydroxide (SBMA) and acrylic acid (AA) in a mixed solution of aluminum chloride (AlCl 3 ) and poly(vinyl alcohol) (PVA) under ultraviolet light (360 nm). PSBMA was switched from a neutral polymer to a positively charged polymer because of the combination of Al 3+ with the negative groups SO 3 − . The water molecules were eliminated because of electrostatic repulsion. The gels exhibited anti-swelling properties (swelling ratio <11%), high stretchability (600% strain), and toughness (2451 kJ/m 3 ). The PPAS-Al 3+ gel was integrated with a wireless Bluetooth system to construct underwater wearable strain sensors that could accurately capture the signals caused by human joint movements and speech recognition even in water. Antibacterial activity (>98.9% inhibition) and stable wireless sensing have potential applications in the fields of wearable sensors, underwater communication, and intelligent healthcare.
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CHINESE JOURNAL OF POLYMER SCIENCE
·
2026
·
IF 4.6
Li Zheng,Yao Mu-Tian,Qiu Zi-Yang,Xu Jing-Kun,Lu Bao-Yang
The development of solar-driven interfacial evaporation technology is pivotal for addressing global water scarcity. However, it is hindered by the difficulty in synergizing high photothermal conversion with low water evaporation enthalpy into a single material. Herein, we propose an iron-aldehyde-cooperative dynamic covalent anchoring strategy, successfully constructing a covalently locked, hydroxymethyl-functionalized PEDOT-PVA integrated dual-network hydrogel (MEPH). This strategy employs Fe 3+ to achieve the one-step in situ oxidative polymerization of hydroxymethyl EDOT while concurrently forming a physical hybrid network with PVA, which is subsequently reinforced by covalent cross-linking using glutaraldehyde. This design endows the MEPH with exceptional broadband light absorption (>99%), efficient water transport, and regulated water state within the hydrogel matrix, leading to a reduced evaporation enthalpy of 732 J·g −1 . The resulting evaporator achieves an ultrahigh evaporation rate of 4.95 kg·m −2 ·h −1 under 1-sun illumination, corresponding to an energy conversion efficiency exceeding 95%, while maintaining stable, salt-resistant operation in high-salinity environments. Outdoor experiments validate its outstanding practicality for seawater and wastewater purification, with the produced freshwater significantly promoting plant growth, highlighting its great potential in sustainable agricultural water cycles. This iron-aldehyde-cooperative dynamic covalent anchoring strategy provides an innovative design paradigm for a new generation of high-performance and robust solar evaporators.
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