Pharmaceutics
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2020
·Vol. 12
·期 6
·574
·
IF 4.421
Qing Min,Xiaofeng Yu,Jiaoyan Liu,Yuchen Zhang,Ying Wan,Jiliang Wu
Thermosensitive alginate–poloxamer (ALG–POL) copolymer with an optimal POL content was synthesized, and it was used to combine with silk fibroin (SF) for building ALG–POL/SF hydrogels with dual network structure. Mesoporous bioactive glass (BG) nanoparticles (NPs) with a high level of mesoporosity and large pore size were prepared and they were employed as a vehicle for loading insulin-like growth factor-1 (IGF-1). IGF-1-loaded BG NPs were embedded into ALG–POL/SF hydrogels to achieve the controlled delivery of IGF-1. The resulting IGF-1-loaded BG/ALG–POL/SF gels were found to be injectable with their sol-gel transition near physiological temperature and pH. Rheological measurements showed that BG/ALG–POL/SF gels had their elastic modulus higher than 5kPa with large ratio of elastic modulus to viscous modulus, indicative of their mechanically strong features. The dry BG/ALG–POL/SF gels were seen to be highly porous with well-interconnected pore characteristics. The gels loaded with varied amounts of IGF-1 showed abilities to administer IGF-1 release in approximately linear manners for a few weeks while effectively preserving the bioactivity of encapsulated IGF-1. Results suggest that such constructed BG/ALG–POL/SF gels can function as a promising injectable biomaterial for bone tissue engineering applications.Keywords:alginate–poloxamer copolymer;silk fibroin;dual network hydrogel;mesoporous bioactive glass;insulin-like growth factor-1
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CHEMICAL ENGINEERING JOURNAL
·
2020
·
IF 10.652
Fengjiao Lai,Feng Yan,Pengju Wang,Shubin Wang,Shun Li,Zuotai Zhang
The design of stable heteropolyacid (HPA) catalysts with strong Brønsted acidity and mild Lewis acidity is crucial for the efficient conversion of renewable biomass to value-added chemicals. Firstly, a novel strategy was proposed to synthesize the Ce-based HPA of H 5 CeW 12 O 40 . Secondly, the H 5 CeW 12 O 40 was combined with ChCl to form a series of temperature-responsive HPA catalysts (Ch n H 5-n CeW 12 O 40 , n = 1–5), which allowed the catalysts to be applied as soluble catalysts at a temperature >80 °C and be easily separated as an insoluble catalyst at a temperature <80 °C. In the H 2 O/DMSO/MIBK biphasic system, one-pot production of 5-hydroxymethylfurfural (5-HMF) from cellulose was achieved with an excellent yield of 67.5% over ChH 4 CeW 12 O 40 catalysis at 140 °C for 6 h, and other carbohydrates were converted, including fructose (83.1%), glucose (74.2%), sucrose (75.8%), cellobiose (70.2%) and starch (68.7%). Such high efficiency was attributed to the homogeneous catalysis at high temperature, the strong Brønsted acidity of [H 5-n CeW 12 O 40 ] n− , and the appropriate Lewis acidity from Ce(III). Moreover, the temperature-responsive property and the high stability were beneficial for recycling without significant structural change and activity loss.
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CHEMICAL ENGINEERING JOURNAL
·
2022
·
IF 16.744
Yichao Liang,Chao Xiong,Xiantai Zhou,Can Xue,Hongbing Ji
A Mo-based catalyst (Mo/CBC) was successfully prepared by a hydrothermal method in which the carbon support was derived from agricultural waste . Physio-chemical properties showed that Mo/CBC had uniformly dispersed particles, large pores, excellent thermal stability and high molybdenum loading. The catalytic performance was systematically investigated, including types of loading metal, oxidants, catalyst usage, substrate concentration, oxidant ratio, reaction temperature, reaction time, reusability, and scalability. Mo/CBC exhibited excellent catalytic performance in the epoxidation of 1-hexene with 98.5% of 1-hexene oxide selectivity, 94.4% of 1-hexene conversion and 99.1% of TBHP consumption efficiency under optimal reaction conditions. The catalytic performance of Mo/CBC did not decrease significantly after five cycles, showing excellent cycle stability. In addition, the scalability showed that Mo/CBC had a good universality for linear olefins, cyclic olefins, aromatic olefins and so on. Thermodynamics showed that the epoxidation system presented as an endothermic and disordered process. Kinetics study showed the rate of the reaction obeyed the pseudo-second-order model with an apparent activation energy of 75.2 kJ/mol. The mechanism study revealed that Mo 4+ species played a key role in the reaction system, promoting the generation of peroxyl radical intermediates from TBHP to directly epoxidize 1-hexene into 1-hexene oxide.
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INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
·
2022
·Vol. 24
·期 1
·335
·
IF 6.208
Yajing Yang,Guoqiang Chen,Xiaoqi Zhao,Xiaohe Cao,Lei Wang,Jingjiu Mu,Fenghui Qi,Lijuan Liu,Haibo Zhang
Exopolysaccharides produced by edible microorganisms exhibit excellent constructive physicochemical and significant biological activity, which provide advantages for the food or pharmaceutical industries. Two novel exopolysaccharides produced byDebaryomyces hanseniiDH-1 were characterized, named S1 and S2, respectively. S1, with a molecular weight of 34.594 kDa, primarily consisted of mannose and glucose in a molar ratio of 12.19:1.00, which contained a backbone fragment of α-D-Manp-(1→4)-α-D-Manp-(1→2)-α-D-Glcp-(1→3)-α-D-Manp-(1→3)-β-D-Glcp-(1→4)-β-D-Manp-(1→. S2, with a molecular weight of 24.657 kDa, was mainly composed of mannose and galactose in a molar ratio of 4.00:1.00, which had a backbone fragment of α-D-Manp-(1→6)-β-D-Manp-(1→2)-α-D-Manp-(1→4)-α-D-Galp-(1→3)-β-D-Manp-(1→6)-α-D-Manp-(1→. Both S1 and S2 exhibited good thermal stability and potent hydroxyl radical scavenging activity, with ~98%. Moreover, S1 possessed an additional strong iron-reducing capacity. In vitro antitumor assays showed that S1 and S2 significantly inhibited the proliferation of Hela, HepG2, and PC-9 cancer cells. Moreover, PC-9 was more sensitive to S1 compared with S2. The above results indicate that S1 and S2 have great potential to be utilized as natural antioxidants and candidates for cancer treatment in the food and pharmaceutical industries.Keywords:Debaryomyces hansenii;exopolysaccharides;structure analysis;antioxidant activity;antitumor activity;thermal property
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Molecular Catalysis
·
2022
·
IF 5.089
Nan Li,Changping Jian,Yaping Song,Li Wang,Ata Ur Rehman,Yanghe Fu,Fumin Zhang,De-Li Chen,Weidong Zhu
Herein, we reported a facile and scalable approach for the synthesis of Fe-based MOF MIL-88A(Fe) via a simple liquid-phase route at atmosphere pressure, and its application as a heterogeneous catalyst for the efficient aerobic oxidation of cyclohexene to value-added 2-cyclohexene-1-ol. Over 130 g of spindle-shaped MIL-88A(Fe) nanocrystals with high crystallinity, monodispersed particle size, and large specific surface area were easily synthesized per batch according to our method. The resultant MIL-88A(Fe) exhibited a high catalytic activity (conversion: 81%) with 70% selectivity for 2-cyclohexene-1-ol in the aerobic oxidation of cyclohexene with O 2 as the sole oxidant under mild reaction conditions (0.5 MPa of O 2 , 353 K for 8 h), outperforming the analogues reported in the literatures. The combined experimental and theoretical approach support the reaction mechanism of the oxidation of cyclohexene via both the free radical reaction pathway and Fe-mediated reaction pathway, resulting in the selective formation of 2-cyclohexene-1-ol.
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Antioxidants
·
2026
·Vol. 15
·期 9
·1217
·
IF 8.2
Yuhong Huang,Meijing Wang,Yiming Zhou,Jiali Liu,Lili Ni,Ya Tan,Mengting Zhang,Jiangpeng Huang,Guolin Li,Fang Wei
Aging is a major risk factor for chronic diseases, driving an urgent need for effective geroprotectors. Repurposing psychotropic agents offers an efficient strategy to accelerate anti-aging therapeutics development. In this study, we investigated the effects of pimozide (a diphenylbutylpiperidine-class antipsychotic) on aging inCaenorhabditis elegansand elucidated the underlying mechanisms. The results demonstrated that 0.1 μM pimozide significantly extends nematode lifespan, increasing mean, median, and maximum lifespans by 44.3%, 50.0%, and 39.8%, respectively. Furthermore, pimozide improves healthspan, as evidenced by reduced age-related lipofuscin accumulation, preserved locomotor function, and decreased paralysis incidence. Moreover, pimozide-treated nematodes showed enhanced resistance to heat, ultraviolet and oxidative stress, concomitant with reduced endogenous reactive oxygen species levels. Transcriptomic profiling and biochemical validation reveal that pimozide markedly upregulates collagen gene expression and increases hydroxyproline content, indicating enhanced collagen synthesis. Mechanistically, pimozide promotes the nuclear localization of SKN-1, and its beneficial effects on lifespan extension and collagen synthesis are SKN-1-dependent, as these effects are abolished inskn-1mutants. Collectively, our findings demonstrate that pimozide promotes longevity and healthspan by activatingskn-1, which is accompanied by enhanced collagen synthesis, providing a preclinical proof of concept for its potential repurposing as a geroprotector.
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JOURNAL OF COLLOID AND INTERFACE SCIENCE
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2023
·
IF 9.9
Chaobo Feng,Bing-Qiang Lu,Yunshan Fan,Haijian Ni,Yunfei Zhao,Shuo Tan,Zhi Zhou,Lijia Liu,Jordan A. Hachtel,Demie Kepaptsoglou,Baohu Wu,Denis Gebauer,Shisheng He,Feng Chen
Amorphous inorganic solids are traditionally isotropic, thus, it is believed that they only grow in a non-preferential way without the assistance of regulators, leading to the morphologies of nanospheres or irregular aggregates of nanoparticles . However, in the presence of (ortho)phosphate (Pi) and pyrophosphate ions (PPi) which have synergistic roles in biomineralization , the highly elongated amorphous nanowires (denoted ACPPNs) form in a regulator-free aqueous solution (without templates, additives, organics, etc). Based on thorough characterization and tracking of the formation process (e.g., Cryo-TEM, spherical aberration correction high resolution TEM, solid state NMR , high energy resolution monochromated STEM-EELS), the microstructure and its preferential growth behavior are elucidated. In ACPPNs, amorphous calcium orthophosphate and amorphous calcium pyrophosphate are distributed at separated but close sites. The ACPPNs grow via either the preferential attachment of ∼2 nm nanoclusters in a 1-dimension way, or the transformation of bigger nanoparticles, indicating an inherent driving force-governed process. We propose that the anisotropy of ACPPNs microstructure, which is corroborated experimentally, causes their oriented growth. This study proves that, unlike the conventional view, amorphous minerals can form via oriented growth without external regulation, demonstrating a novel insight into the structures and growth behaviors of amorphous minerals.
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2023
·
IF 8.2
Xiaopeng Huang,Fangfang Wang,Wenkai Hu,Zhiming Zou,Qun Tang,Heping Li,Lin Xu
There is at present an acute need for the construction of biopolymer-based smart packaging material that can be applied for the real-time visual monitoring of food freshness. Herein, a nano-sized substituted imidazolate material (SIM-1) with ammonia-sensitive and antibacterial ability was effectively manufactured and then anchored within corn starch/polyvinyl alcohol (CS/PVA) blend to construct biopolymeric smart active packaging material. The structure, physical and functional performances of CS/PVA-based films with different content of SIM-1 (0.5, 1.0 and 2.0 wt% on CS/PVA basis) were then explored in detail. Results revealed that the incorporated SIM-1 nanocrystals were equally anchored within the CS/PVA matrix owing to the establishment of potent hydrogen-bonding interactions, which produced an obvious improvement in the compatibility of CS/PVA blend film, as well as its mechanical strength , water/oxygen barrier and UV-screening performances. The constructed CS/PVA/SIM-1 blend films further demonstrated superior long-term color stability property, ammonia-sensitive and antibacterial functions. Furthermore, the CS/PVA/SIM-1 blend films were utilized for effectively monitoring the deterioration of shrimp via observable color alteration. The above findings suggested the potential applications of CS/PVA/SIM-1 blend films in smart active packaging.
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PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY
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2023
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IF 4.7
Haitong Wu,Haoye Li,Haihua Huo,Xinrun Li,Heyun Zhu,Lijiao Zhao,Jianzhao Liao,Zhaoxin Tang,Jianying Guo
Terbuthylazine (TBA) is one of the most commonly used and effective herbicides. However, due to its affinity for soil organic matter and water solubility, TBA can lead to biological health concerns. This study exposed broilers to TBA (0 mg/kg bw, 0.4 mg/kg bw, 4 mg/kg bw) for 28 days. The results showed significant pathological damage in broiler myocardial tissue, such as widening of the interstitial space, rupture of muscle fibers, and deposition of myocardial collagen fibers. In addition, Under the 0.4 mg/kg bw TBA exposure, myocardial oxidative stress was observed in broilers, which was accompanied by the activation of Nrf2/HO-1 pathway and the increased protein and mRNA levels of NQO1, NOX2 and SOD2 antioxidant enzymes . However, Nrf2/HO-1 protein and mRNA levels were reversed at 4 mg/kg bw TBA exposure. Meanwhile, the Nrf2/HO-1 mediated antioxidant defense was impaired. In contrast with the low dose, the protein and gene expression levels of NQO1, NOX2, and SOD2 were reduced in 4 mg/kg bw TBA group. The expression of GPX4 and SLC7A11 was significantly downregulated at both protein and mRNA levels. Beyond that, ACSL4 expression was significantly up-regulated, and the protein result was consistent with the mRNA expression, demonstrating the occurrence of ferroptosis . In general, TBA exposure activated the Nrf2/HO-1 pathway, resulting in ferroptosis . This study links ferroptosis to the Nrf2/HO-1 pathway, providing new insights into the potential role of TBA in myocardial toxicity.
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DIGESTIVE DISEASES AND SCIENCES
·
2023
·
IF 3.1
Tian Xiaorong,Wang Yuxin,Yuan Mengshu,Zheng Weiping,Zuo Huaiwen,Zhang Xinru,Song Hongli
Background Ischemia–reperfusion injury (IRI) is an important cause of graft dysfunction post-liver transplantation, where donor liver with severe steatosis is more sensitive to IRI. Liver IRI involves ferroptosis and can be alleviated by heme oxygenase-1-modified bone marrow mesenchymal stem cells (HO-1/BMMSCs). Aims To explore the role and mechanism of HO-1/BMMSCs in severe steatotic liver IRI. Methods A severe steatotic liver IRI rat model and a hypoxia/reoxygenation (H/R) of severe steatosis hepatocyte model were established. Liver and hepatocyte damage was evaluated via liver histopathology and cell activity. Ferroptosis was evaluated through ferroptosis indexes. Nuclear factor erythroid 2-related factor 2 (Nrf2) was knocked down in severe steatotic hepatocytes. The role of Nrf2 and AMPK in HO-1/BMMSC inhibition of ferroptosis was examined using the AMP-activated protein kinase (AMPK) pathway inhibitor Compound C. Results The HO-1/BMMSCs alleviated severe steatotic liver IRI and ferroptosis. HO-1/BMMSCs promoted ferritin heavy chain 1(FTH1), Nrf2, and phosphorylated (p)-AMPK expression in the H/R severe steatotic hepatocytes. Nrf2 knockdown decreased FTH1 expression levels but did not significantly affect p-AMPK expression levels. The protective effect of HO-1/BMMSCs against H/R injury in severe steatotic hepatocytes and the inhibitory effect on ferroptosis were reduced. Compound C decreased p-AMPK, Nrf2, and FTH1 expression levels, weakened the HO-1/BMMSC protective effect against severe steatotic liver IRI and H/R-injured severe steatotic hepatocytes, and reduced the inhibition of ferroptosis. Conclusions Ferroptosis was involved in HO-1/BMMSC reduction of severe steatotic liver IRI. HO-1/BMMSCs protected against severe steatotic liver IRI by inhibiting ferroptosis through the AMPK–Nrf2–FTH1 pathway. Graphical Abstract HO-1/BMMSCs activate AMPK, which activates Nrf2, promotes its nuclear transcription, then promotes the expression of its downstream protein FTH1, thereby inhibiting ferroptosis and attenuating severe steatotic liver IRI in rats. Glu: glutamic acid; Cys: cystine; GSH: glutathione; GPX4: glutathione peroxidase 4; HO-1/BMMSCs: HO-1-modified BMMSCs; Fer-1: ferrostatin-1; DFO: deferoxamine; FTH1: ferritin heavy chain1; p-AMPK: phosphorylated AMP-activated protein kinase; Nrf2: nuclear factor erythroid 2-related factor 2; IRI: ischemia-reperfusion injury; MCD: methionine-choline deficiency
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Membranes
·
2023
·Vol. 13
·期 9
·781
·
IF 4.2
Jiaming Gao,Yongchao Sun,Feifei Kang,Fei Guo,Gaohong He,Hanli Wang,Zhendong Yang,Canghai Ma,Xiaobin Jiang,Wu Xiao
Mixed matrix membranes (MMMs) generally have some fatal defects, such as poor compatibility between the two phases leading to non-selective pores. In this work, PIM-1 was chosen as the polymer matrix, and UiO-66 modified with amidoxime (UiO-66-AO) was used as the filler to prepare the MMMs. In the MMMs, the amino and hydroxyl groups on UO-66-AO form a rich hydrogen bond network with the N and O atoms in the polymer PIM-1 chain to improve the compatibility between the polymer matrix and the filler. In addition, the selective adsorption of CO2by the amidoxime group can promote the transport of CO2in the membrane, which enhances the gas selectivity. The CO2permeability and CO2/N2selectivity of UiO-66-AO@PIM-1 MMMs are increased by 35.2% and 45.2% compared to pure PIM-1 membranes, reaching 7535.5 Barrer and 26.9, surpassing the Robeson Upper Bound (2008) and close to the 2019 Upper Bound. After 38 days of the aging experiment, the CO2permeability is approximately 74% of the original. The results show that the addition of UiO-66-AO has an obvious effect on improving the aging properties of the membrane. The UiO-66-AO@PIM-1 MMMs have a bright prospect for CO2separation in the future.Keywords:amidoxime;UiO-66-AO;mixed matrix membrane;PIM-1;CO2/N2separation
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DNA AND CELL BIOLOGY
·
2023
·Vol. 42
·期 9
·554-562
·
IF 3.1
Liangliang Yang,Yang Gao,Jinlong Huang,Hantao Yang,Puyuan Zhao,Chen Li,Zhigang Yang
Inhibition of the inflammatory response triggered by microglial pyroptosis inflammatory activation may be one of the effective ways to alleviate cerebral ischemia–reperfusion injury, the specific mechanism of which remains unclear. In this study, BV-2 microglia with or without oxygen–glucose deprivation/reoxygenation (OGD/R) or long noncoding RNA (lncRNA) Gm44206 knockdown were used as cell models to conduct an in vitro study. Detection of lactate dehydrogenase release and pyroptosis-related protein levels was performed using a corresponding kit and western blotting, respectively. Proliferation of microglia was evaluated by CCK8 assay. Enzyme-linked immunosorbent assay was applied for measuring levels of proinflammatory cytokines. This study verified the involvement of microglial pyroptosis as well as upregulation of NLRP3, Caspase-1, GSDMD, and Apoptosis-associated Speck-like protein containing a C-terminal caspase-recruitment domain (ASC) in cerebral ischemia–reperfusion injury. Moreover, knockdown of lncRNA Gm44206 could alleviate OGD/R-induced microglial pyroptosis and cell proliferation inhibition through the NLRP3/Caspase-1/GSDMD pathway, thus decreasing the release of proinflammatory cytokines, including interleukin (IL)-1β, IL-6, IL-18, and tumor necrosis factor-alpha. In conclusion, this study established a correlation between microglial pyroptosis and cerebral ischemia–reperfusion injury and identified lncRNA Gm44206 as a potential regulator of NLRP3/Caspase-1/GSDMD axis-mediated microglial pyroptosis, which could be considered a promising therapeutic target.
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