VETERINARY RESEARCH COMMUNICATIONS
·
2023
·
IF 2.2
Zeng Yan,Yin Hongmei,Zhou Xiaoling,Wang Chunping,Zhou Bingyu,Wang Bin,Tang Bingxuan,Huang Lihong,Chen Xian,Zou Xiaoyan
This study aimed to investigate the effects of replacing of dietary inorganic iron with iron-rich Lactobacillus plantarum and iron-rich Candida utilis on the growth performance, serum parameters, immune function and iron metabolism of weaned piglets. Fifty-four 28-day-old healthy Duroc × Landrace × Yorkshire castrated male weanling piglets of similar body weight were randomly and equally divided into three groups. The piglets were kept in three pens per group, with six pigs in each pen. The dietary treatments were (1) a basal diet + ferrous sulfate preparation containing 120 mg/kg iron (CON); (2) a basal diet + iron-rich Candida utilis preparation containing 120 mg/kg iron (CUI); and (3) a basal diet + iron-rich Lactobacillus plantarum preparation containing 120 mg/kg iron (LPI). The entire feeding trial lasted for 28 days, after which blood, viscera, and intestinal mucosa were collected. The results showed no significant difference in growth parameters and organ indices of the heart, liver, spleen, lung, and kidney of weaned piglets when treated with CUI and LPI compared with the CON group ( P > 0.05). However, CUI and LPI significantly reduced the serum contents of AST, ALP, and LDH ( P < 0.05). Serum ALT content was significantly lower in the LPI treatment compared to the CON group ( P < 0.05). Compared to CON, CUI significantly increased the contents of serum IgG and IL-4 ( P < 0.05), and CUI significantly decreased the content of IL-2. LPI significantly increased the contents of serum IgA, IgG, IgM and IL-4 ( P < 0.05), while LPI significant decreased the levels of IL-1β, IL-2, IL-6, IL-8, and TNF-α compared to CON ( P < 0.05). CUI led to a significant increase in ceruloplasmin activity and TIBC ( P < 0.05). LPI significantly increased the contents of serum Fe and ferritin, and increased the serum ceruloplasmin activity and TIBC compared to CON ( P < 0.05). Furthermore, CUI resulted in a significant increase in the relative mRNA expression of FPN1 and DMT1 in the jejunal mucosa ( P < 0.05). LPI significantly increased the relative mRNA expression of TF , FPN1 , and DMT1 in the jejunal mucosa ( P < 0.05). Based on these results, the replacement of dietary inorganic iron with an iron-rich microbial supplement could improve immune function, iron absorption and storage in piglets.
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MICROCHIMICA ACTA
·
2023
·Vol. 191
·期 1
·1-11
·
IF 5.7
Shi Fengna,Hu Sheng,Li Jingfang,Wang Fang,Chen Naipin
A novel, green, and facile approach has been developed to construct an ultrasensitive flexible enzyme-less electrochemical sensor on the basis of chitosan and graphene oxide composites decorated with Cu nanoparticles supported on nickel foam (Nif/Cs/GO@Cu), in which GO functions as the intermediate between Nif and Cu nanoparticles. The Nif/Cs/GO@Cu sensing platform was successfully fabricated by the drop casting method to load Cs/GO onto Nif followed by an additionally electrodeposition to support Cu nanoparticles on Nif/Cs/GO. Impressively, the Nif/Cs/GO@Cu exhibited much higher electrocatalytic activity for glucose and UA oxidation as compared to that of Nif or Nif@Cu. For glucose and UA at about 0.6 V and 0.1 V (vs. Ag/AgCl), linearity could be obtained in the concentration ranges 5 µM–4 mM and 5–345 µM; the sensitivities were 16 and 2.5 µA µM −1 cm −2 , and the detection limits 83 nM and 0.3 µM, respectively. The improved performance of the composite electrode was ascribed to the synergistic effect of Cu nanoparticles, Nif and GO, in which GO provides high electron conductivity and large surface area to prevent the agglomeration of Cu nanoparticles; Cu nanoparticles and Nif offer abundant active sites towards analytes oxidation. Additionally, the method was applied to determine both analytes successfully in blood serum samples with excellent recovery and also opens up an attractive route to potential applications of the flexible nickel foam-based electrochemical sensor. Graphical Abstract
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ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
·
2023
·Vol. 30
·期 39
·91262-91275
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IF 5.8
Cheng Tangying,Li Jinjin,Ma Xiuwei,Yang Linjun,Zhou Lei,Wu Hao
Various materials have been developed to capture volatile organic compounds (VOCs) to mitigate air pollution. However, sorbent materials with excellent resistance to water are rare. Here, several Fe/N-doped activated carbons (ACs) have been prepared to capture VOCs in humid environments. The ACs were analyzed by various characterization techniques, such as BET, SEM, XPS, XRD, FTIR, and Raman. The results showed that Fe/N doping resulted in the specific surface area of the ACs increasing by 500 to 1000 m 2 g −1 , the average pore size increasing to approximately 2 nm, improved mesoporous structure, higher graphitization, lower hydrophilicity, and polarity. The VOCs adsorption performance of the ACs was evaluated by static and dynamic adsorption experiments. The uptake of toluene and ethyl acetate by ACs was enhanced to 224 mg g −1 and 135 mg g −1 , respectively. And ACs were able to maintain 70 to 80% VOCs adsorption capacity for VOCs at 80% relative humidity. Furthermore, the microscopic mechanisms were investigated by the grand canonical Monte Carlo method (GCMC). The highly graphitized structure and the N functional groups favored the VOC adsorption process and discouraged the adsorption of water vapor. This work affirmed the dominance of Fe/N-doped carbon, which will contribute to the evolution of water-resistant VOCs adsorbent materials. Graphical Abstract
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JOURNAL OF MATERIALS SCIENCE-MATERIALS IN ELECTRONICS
·
2023
·Vol. 34
·期 18
·1-13
·
IF 2.8
Li Chaojie,Feng Yonghang,Cui Yi,Zhang Fan,Lv Dongfeng,Liu Pan,Wei Yingna,Wei Hengyong,Bu Jinglong
The NiCo 2 O 4 nanoflakes/SiC fibers composites were prepared by electrospinning, carbon thermal reduction combined with hydrothermal method. The results of SEM, XRD and XPS suggested that the NiCo 2 O 4 nanoflakes/SiC fibers composites were successfully prepared. The NiCo 2 O 4 nanoflakes were generated on the surface of SiC fibers with uniform distribution and loose structure. The NiCo 2 O 4 nanoflakes/SiC fibers composites possess the porous structure and their BET specific surface area is 233.2 m 2 /g. The BJH average pore size and the pore volume are 7.0 nm and 0.4 cm 3 /g, respectively. The structure of fibers and pores can bring the multiple reflections and scatterings, interface polarizations, dipole polarizations and conductivity losses. In addition, there is the synergistic effect of dielectric loss and the magnetic loss in NiCo 2 O 4 nanoflakes/SiC fibers composites. Therefore, the reflection loss of NiCo 2 O 4 nanoflakes/SiC fibers composites could achieve − 56 dB under the thickness of 6 mm at 17.64 GHz, which showed the better microwave absorption behavior than SiC fibers.
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Nano Research
·
2023
·Vol. 16
·期 8
·11139-11148
·
IF 9.9
Li Na,Liu Wan,Zheng Xiaoyan,Wang Qing,Shen Lixin,Hui Junfeng,Fan Daidi
Burns are a common medical problem globally, and wound infection is one of the major causes of inducing related complications. Although antibiotics effectively prevent wound infections, the misuse of antibiotics has created a new problem of superbugs. Herein, we propose a new strategy to obtain pH-responsive antimicrobial P-ZIF (ZIF: zeolitic imidazolate framework) by loading polyhexamethylenebiguanide (PHMB) into the framework of ZIF-8 nanoparticles. This will enable PHMB to be released in the weak acid environment of an infected wound. To address burn infections, P-ZIF nanoparticles were loaded into a hydrogel system made of sodium alginate (SA) and 3-aminophenylboronic acid modified human-like collagen (H-A) through borate ester bonds. The resulting H-A/SA/P-ZIF (HASPZ) hydrogel dressing not only possesses antibacterial and wound healing properties but also has dual pH responsiveness to prevent the overuse of medication while effectively treat deep second-degree burns. Therefore, P-ZIF nanoparticles and the corresponding HASPZ hydrogel dressing are considered of significant importance in antimicrobial, drug delivery, and wound repair.
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Nature Communications
·
2023
·Vol. 14
·期 1
·1-14
·
IF 16.6
Xu Shichao,Wu Haifeng,Liu Siyuan,Du Peidong,Wang Hui,Yang Haijun,Xu Wenjie,Chen Shuangming,Song Li,Li Jikun,Shi Xinghua,Wang Zhen-Gang
Enzymes fold into unique three-dimensional structures to distribute their reactive amino acid residues, but environmental changes can disrupt their essential folding and lead to irreversible activity loss. The de novo synthesis of enzyme-like active sites is challenging due to the difficulty of replicating the spatial arrangement of functional groups. Here, we present a supramolecular mimetic enzyme formed by self-assembling nucleotides with fluorenylmethyloxycarbonyl (Fmoc)-modified amino acids and copper. This catalyst exhibits catalytic functions akin those of copper cluster-dependent oxidases, and catalytic performance surpasses to date-reported artificial complexes. Our experimental and theoretical results reveal the crucial role of periodic arrangement of amino acid components, enabled by fluorenyl stacking, in forming oxidase-mimetic copper clusters. Nucleotides provide coordination atoms that enhance copper activity by facilitating the formation of a copper-peroxide intermediate. The catalyst shows thermophilic behavior, remaining active up to 95 °C in an aqueous environment. These findings may aid the design of advanced biomimetic catalysts and offer insights into primordial redox enzymes.
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Carbon Letters
·
2023
·Vol. 33
·期 6
·1661-1667
·
IF 4.5
Tian Tingyu,Cai Yuping,Yang Shimao,Guo Yanwei,Zhou Wei
In this study, we successfully grafted chitosan (CS) onto multi-walled carbon nanotubes (MWCNTs) to enhance their properties and potential applications in the biomedical field. FTIR spectroscopy confirmed the successful covalent bonding of CS onto MWCNTs, indicated by the new absorption peak of the amide bond (–CONH–). Thermal analysis showed that the modified MWCNTs (MWCNT-CS) had significant weight loss around 260 °C, suggesting the decomposition of hydroxypropyl chitosan, and confirming its presence in the nanocomposite. SEM images revealed that CS grafting improved the dispersibility of MWCNTs, a property crucial for their use as nanofillers in polymers. Moreover, the micro-tensile bond strength of dentin surface increased with increasing MWCNT-CS concentrations, indicating the potential of MWCNT-CS as a pretreatment for dentin bonding. After simulated aging, the bond strength remained significantly higher for MWCNT-CS groups compared to those without pretreatment. In biocompatibility assessment using the MTT assay, MWCNT-CS showed higher cell viability than MWCNT, suggesting improved biocompatibility after CS modification. The results of this study suggest that CS-modified MWCNTs could be promising materials for applications in dentin bonding, dentin mineralization, bone scaffolding, implants, and drug delivery systems.
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Journal of Central South University
·
2023
·Vol. 30
·期 5
·1512-1522
·
IF 4.4
Dong Zhao-wang,Duan Liang-hong,Liu Han-ning,Guo Xue-yi,Xia Yang
High-purity zirconium metal has been of rising significance to the nuclear and energy industry. However, zirconium exhibits a strong chemical affinity to oxygen, which causes high-purity zirconium metal to be difficult to produce cost-effectively. Powder metallurgy for preparing zirconium metal can achieve near net forming of zirconium metal. Zirconium powder can be prepared by deoxidation of zirconium hydride powder. In this study, an efficient process was proposed to prepare high-purity ZrH 2 using magnesium powder as reducing agent in hydrogen atmosphere. A specific investigation was conducted on the effect of hydrogen on the magnesiothermic reduction and deoxidation of ZrO 2 . As indicated from the results, the oxygen content of the reduced powder in Ar was over three times that in H 2 atmosphere at 750 °C for 240 min. Moreover, a passive oxide layer, with a thickness of ~2.76 nm in H 2 and of ~8.51 nm in argon, was formed on the edge of the reduced powder. In accordance with thermodynamic calculation, hydrogen was found to be capable of destabilizing Zr-O solution. An ultra-high pure zirconium hydride powder containing <350×10 −6 oxygen and particle size of <100 µm was prepared as assisted by hydrogen.
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BIOLOGICAL TRACE ELEMENT RESEARCH
·
2023
·
IF 3.9
Peng Huixin,Huang Yanxin,Wei Guangji,Pang Yanfang,Yuan Huixiong,Zou Xiong,Xie Yu’an,Chen Wencheng
Aluminum contamination is a growing environmental and public health concern, and aluminum testicular toxicity has been reported in male rats; however, the underlying mechanisms of this toxicity are unclear. The objective of this study was to investigate the effects of exposure to aluminum chloride (AlCl 3 ) on alterations in the levels of sex hormones (testosterone [T], luteinizing hormone [LH], and follicle-stimulating hormone [FSH]) and testicular damage. Additionally, the mechanisms of toxicity in the testes of AlCl 3 -exposed rats were analyzed by proteomics. Three different concentrations of AlCl 3 were administered to rats. The results demonstrated a decrease in T, LH, and FSH levels with increasing concentrations of AlCl 3 exposure. HE staining results revealed that the spermatogenic cells in the AlCl 3 -exposed rats were widened, disorganized, or absent, with increased severe tissue destruction at higher concentrations of AlCl 3 exposure. Kyoto Encyclopedia of Genes and Genomes (KEGG) and Gene Ontology (GO) enrichment analyses revealed that differentially expressed proteins (DEPs) after AlCl 3 exposure were primarily associated with various metabolic processes, sperm fibrous sheath, calcium-dependent protein binding, oxidative phosphorylation, and ribosomes. Subsequently, DEPs from each group were subjected to protein-protein interaction (PPI) analysis followed by the screening of interactional key DEPs. Western blot experiments validated the proteomics data, revealing the downregulation of sperm-related DEPs (AKAP4, ODF1, and OAZ3) and upregulation of regulatory ribosome-associated protein (UBA52) and mitochondrial ribosomal protein (MRPL32). These findings provide a basis for studying the mechanism of testicular toxicity due to AlCl 3 exposure.
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JOURNAL OF CANCER RESEARCH AND CLINICAL ONCOLOGY
·
2023
·
IF 3.6
Zhang Yang,Chao Fengmei,Lv Lihua,Li Ming,Shen Zuojun
Purpose To explore the potential of circRNAs as biomarkers in non-invasive body fluids for monitoring chemotherapy resistance in SCLC patients. Methods CircRNAs were screened and characterized using transcriptome sequencing, Sanger sequencing, actinomycin D treatment, and Ribonuclease R assay. Our study involved 174 participants, and serum samples were collected from all chemotherapy-resistant patients (n = 54) at two time points: stable disease and progressive disease. We isolated and identified serum extracellular vesicles (EVs) from the patients using ultracentrifugation, transmission electron microscopy, nanoflow cytometry, and western blotting analysis. The expression levels of serum and serum EVs circRNAs were determined by quantitative real-time polymerase chain reaction (qRT-PCR). The impact of circRNA on the function of SCLC cells was assessed through various assays, including proliferation assay, scratch assay, transwell assay, and cisplatin resistance assay. Results Hsa_circ_0041150 was found to be upregulated in chemoresistant SCLC cells and played a role in promoting proliferation, invasion, migration, and cisplatin resistance. Furthermore, the expression levels of hsa_circ_0041150 in serum and serum EVs increased when SCLC patients developed resistance after a first-line chemotherapy regimen. When combined with NSE, the monitoring sensitivity (70.37%) and specificity (81.48%) for chemotherapy resistance significantly improved. Moreover, the expression level of hsa_circ_0041150 showed significant associations with time to progression from SD to PD, and high hsa_circ_0041150 levels after drug resistance were more likely to cause chemotherapy resistance. Additionally, hsa_circ_0041150 demonstrated valuable potential in monitoring the progression from initial diagnosis to chemotherapy resistance in SCLC patients. Conclusion Thus, EVs hsa_circ_0041150 holds promise as a biomarker for monitoring chemotherapy resistance in SCLC patients.
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PLANT CELL TISSUE AND ORGAN CULTURE
·
2023
·
IF 3
Zhang Ting,Zhao Yichen
Tea plant ( Camellia sinensis (L.) O. Kuntze) is an important woody economic crop that has been cultivated worldwide. Adversity stress acts as a significant factor affecting the growth and development of tea plants. Brassinazole-resistant (BZR) family protein is an essential factor in regulating plant stress resistance and plays a key role in cold stress response, heat, drought and salt resistances. In this study, the promoter region upstream of CsBZR1-2 was cloned. The promoter sequence and its 5′-end truncated segment were inserted upstream of β-glucuronidase ( GUS ) gene in the pCambia1391Z plasmid. Stably expressed transgenic tobacco lines were obtained by Agrobacterium tumefaciens -mediated genetic transformation. The results showed that except for promoter lacking the core element, both full-length and truncated promoters could initiate the expression of GUS , but the level of expression was affected by the degree of deletion at the 5′-end. The core region of the promoter was in a 311 bp region including the transcription start site (TSS). In addition, the regulatory mechanism of the promoter was explored. It was found that the genes regulated by the promoter were responsive to stress such as cis-acting elements (MeJA, ABRE and LTR), BR and light. This paper provides a theoretical basis to further explore the regulatory mode of the promoter and to achieve efficient gene expression.
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JOURNAL OF MATERIALS SCIENCE-MATERIALS IN ELECTRONICS
·
2023
·Vol. 34
·期 21
·1-16
·
IF 2.8
Zi Xingfu,Wu Hongming,Song Jiling,Guo Guangping,Guo Jianbing
Cellulose-based gel electrolytes used in Li-ion batteries have poor interfacial compatibility, leading to poor cycle and rate performance. Herein, the preparation of a cellulose/polyethylene glycol dimethacrylate (PEGDA) gel electrolyte, with good cycle stability, is reported. The interfacial compatibility, electrolyte uptake, and thermal stability of the gel electrolyte could be adjusted by changing the PEGDA content of the gel electrolyte. The porosity changed with the PEGDA content and the electrolyte uptake rate increased from 383.49 to 932.57%. The interfacial resistance of the cellulose/PEGDA gel electrolyte was 97.24 Ω, while that of the pure cellulose was 565.06 Ω. This can be attributed to the added PEGDA, which hinders the hydroxy activity of the cellulose, thus, improving the electrode–electrolyte interface performance. The excellent interfacial compatibility and high porosity led to excellent cycle stability. The capacity retention rate of the assembled battery after 100 cycles, at 0.5 C, was as high as 96.8%. In addition, the high electrolyte uptake rate from the 3D porous structure also improved the ionic conductivity and Li-ion migration number of the cellulose/PEGDA gel electrolyte.
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