ACS Applied Nano Materials
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2022
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IF 6.14
Yuting Liao,Dong Liu,Bin Wang,Zijian Wu,Miao Ni,Mengchen Wang,Cheng-Hua Wang,Yuan Lu
The intensification of microreactions is important for biological and chemical reactions with microliter volumes as reaction units, including microliter bioassay or protein synthesis. At the micro/nanoscale, the fluid flow is usually located in the laminar region, making the interaction between different layers of material more difficult. In the absence of external intervention, the microscale transport of substances is dominated by passive diffusion, leading to a lower mixing efficiency and longer reaction times, which is disadvantageous for reactions requiring timeliness, such as microliter bioassays. Therefore, the intensification of microreactions is essential. Here, a dual magnetic–photothermal microreaction control platform was created, based on the cooperative roles of a close-loop photothermally controlled magnetically active nanoswarm (PMANS) as a nanostir for quick fluid mixing and as a nanoheating agent for the reaction solution. The platform was demonstrated to be enhanced for microreactions by using the glucose oxidase and horseradish peroxidase cascade and cell-free synthetic superfolder green fluorescent protein reactions, which are typical in the fields of diagnostic testing and biomanufacturing, respectively. The rotational motion of the PMANS is controlled by the magnetic field to achieve efficient and homogeneous mixing of fluids at the microscale. On the other hand, the PMANS is used to heat the reaction system through light control. The construction of a dual magnetic–temperature control platform provided a new strategy for microreaction enhancement that was convenient, controllable, and broadly applicable. This control platform has great potential for microscale reaction enhancement in detection, sensing, catalysis, and synthesis.
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ACS Sensors
·
2023
·
IF 8.9
Hua-Ying Chen,Pei-Lin Xin,Han-Bin Xu,Jian Lv,Ruo-Can Qian,Da-Wei Li
Herein, a fluorescence and surface-enhanced Raman spectroscopy dual-mode system was designed for cholesterol detection based on self-assembled plasmonic nanojunctions mediated by the competition of rhodamine 6G (R6G) and cholesterol with β-cyclodextrin modified on gold nanoparticles (HS-β-CD@Au). The fluorescence of R6G was quenched by HS-β-CD@Au due to the fluorescence resonance energy transfer effect. When cholesterol was introduced as the competitive guest, R6G in the cavities of HS-β-CD@Au was displaced to recover its fluorescence. Moreover, two of HS-β-CD@Au can be linked by one cholesterol to form a more stable 2:1 complex, and then, plasmonic nanojunctions were generated, which resulted in the increasing SERS signal of R6G. In addition, fluorescence and SERS intensity of R6G increased linearly with the increase in the cholesterol concentrations with the limits of detection of 95 and 74 nM, respectively. Furthermore, the dual-mode strategy can realize the reliable and sensitive detection of cholesterol in the serum with good accuracy, and two sets of data can mutually validate each other, which demonstrated great application prospects in the surveillance of diseases related with cholesterol.
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CANCER LETTERS
·
2023
·
IF 9.7
Xiaosong Wei,Beibei Wang,Zixin Wu,Xiaoming Yang,Yufeng Guo,Yang Yang,Zhiwei Fang,Chengzhi Yi,Liuhui Zhang,Xin Fan,Lirong Zhang,Dongkui Song
One of the most abundant protein-protein interaction domains in the human proteome is the WD40 repeat (WDR) domain. A Gene Expression Omnibus dataset revealed 37 differentially expressed WDR domain genes in bladder cancer (BC). WD repeat domain 54 ( WDR54 ), an upregulated WDR domain gene, was selected for further investigation. Sixty pairs of frozen BC tumor and non-malignant bladder tissues and 83 paraffin-embedded BC tissue specimens were obtained. Loss-/gain-of-function experiments were carried out using BC and xenograft tumor models. WDR54 was overexpressed in BC cells, and its high expression was linked to tumor stage and lymph node metastases in patients. WDR54 contributed to the tumorigenesis and metastasis of BC and impaired its chemosensitivity. WDR54 prevented the degradation and ubiquitination of the mediator of ErbB2-driven cell motility 1 (MEMO1). WDR54 also promoted the interaction between MEMO1 and insulin receptor substrate 1 (IRS1) and activated the IRS1/AKT/β-catenin pathway in BC cells. Particularly, WDR54 depended on MEMO1 to exert its biological functions. Our study demonstrated the relevance of WDR54 in BC and provides insight into the molecular mechanism underlying BC.
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Frontiers in Chemistry
·
2022
·
IF 5.545
Cui Xiang,Deng Shanshan,Li Guoyin,Zhang Yunxia,Wang Lining,Wu Changjing,Deng Yanru
Two undescribed butenolide derivatives, asperteretal J (1) and K (2), together with 13 known ones (3–15) were isolated from an endophytic fungus Aspergillus terreus SGP-1, the fermentation product of which exhibited selective inhibitory activity toward butyrylcholinesterase. The structures of the new compounds were elucidated based on HRMS and NMR data, and the absolute configurations were determined by specific optical rotation comparison. All compounds were evaluated for cholinesterase inhibitory effects with galantamine as a positive control. Compounds 4–8 selectively inhibited butyrylcholinesterase with IC50 values of 18.4–45.8 µM in a competitive manner, with Ki values of 12.3–38.2 µM. The structure-activity relationship was discussed. Molecular docking and dynamic simulation of the inhibitor-enzyme complex were performed to better understand the interactions.
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COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
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2022
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IF 5.518
Caixin Guo,Donghao Liu,Wenbo Xu,Liangcan He,Shaoqin Liu
The peroxidase (POD)- and glucose oxidase (GOx)-like activity of Au nanoparticles (AuNPs) is very low compared to that of natural enzymes, which greatly limits their application in biological field. Herein, we found that the enlargement of AuNPs by dopamine-mediated seeded growth strategy could significantly accelerate the POD- and GOx-like activity of AuNPs. The increase of the dual enzyme-like activities may be ascribed to the formation of branched Au superstructures in the seeded growth process. With the enhancement of dual enzyme-like activities of AuNPs, a sensitive and selective colorimetric detection of dopamine and glucose can be achieved respectively. This work is expected to provide a general method to synthesize other metal-based nanomaterials with branched superstructures and extend its application in areas such as medical diagnosis and therapy.
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Food Science and Technology
·
2022
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IF 2.602
MO Mengmiao,JIANG Fengyu,CHEN Weiming,DING Zhendong,BI Yongguang,KONG Fansheng
Three new polysaccharides (SLP-50, SLP-70, and SLP-90) were fractionated successively by gradient concentrations of ethanol. This study aimed to investigate the initial structural characterization and bioactivities of these sugarcane leaves polysaccharides (SLPs) obtained by ultrasound-assisted extraction. The results, which were further validated by IR spectrometry, revealed that the three SLPs contained uronic acids, proteins, and sulfate groups in addition to carbohydrate. SLP-50 (36.40 kDa), SLP-70 (12.97 kDa), and SLP-90 (3.52 kDa) were acidic heteropolysaccharides mainly comprised of mannose (Man), rhamnose (Rha), glucuronic acid (Glc A), galacturonic acid (Gal A), galactose (Gal), glucose (Glc), xylose (Xyl), and arabinose (Ara), in different molar ratios. SLP-90 exhibited higher antioxidant capacity than SLP-50 and SLP-70. In vitro, all fractions showed significant hypoglycemic potential and antibacterial activity against Gram-positive and Gram-negative bacteria. The IC50 values of SLP-50, SLP-70, and SLP-90 were 0.11, 0.05, and 0.67 mg/mL on α-glucosidase, respectively, which were significantly lower than that of acarbose (0.80 mg/mL). These findings could provide a reference for developing and applying SLP-based functional foods.
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Biomaterials Advances
·
2023
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IF 0
Yiqing Liu,Peiyong Guo,Xinhao Dong,Yina Xu,Dan Li,Hua Zheng,Jianhong Liao
Here, we combined reversible addition-fragmentation chain transfer (RAFT) polymerization and amide coupling reaction to develop a novel drug-polymer conjugate using poly(AMA- co -IMMA)-b-poly(OEGMA) (termed as PAIPO) as nanocarriers. In order to enhance cellular uptake and obtain subsequent endo/lysosomal escape capacity, the dual-drugs-conjugated prodrug was then coupled with 2,3-dimethylmaleimide (DA) moieties and implanted with imidazolyl groups, respectively. Paclitaxel (PTX) was conjugated to PAIPO via 3,3′-dithiodipropionic acid (DPA) to construct a GSH-responsive moiety, while doxorubicin (DOX) was conjugated to PAIPO via 4-formyl benzoic acid to construct a pH-responsive moiety, which synergistically enabled a synchronized and precise drug delivery. The micelles self-assembled from DOX/ [email protected] DA showed an ideal average diameter (163.2–178.3 nm), contributing to passive targeting by the EPR effect. Moreover, a switch of the surface Zeta potential of micelles from steady negatively charged (− 9.74 ± 0.54 mV) at pH 7.4 to positively charged (+ 6.33 ± 1.25 mV) at pH 6.5, facilitated the long blood circulation and cellular endocytosis of micelles, respectively. More importantly, in vitro studies confirmed that DA M(DOX n /PTX) exhibited a strong synergism against tumor cells, and under slightly acidic conditions (pH 6.5), the combination index (CI) values for DA M(DOX 1 /PTX) on HeLa and Skov-3 cells were estimated to be 0.47 and 0.49 (previous to be 0.50 and 0.56 at pH 7.4), respectively. And in vivo results showed effective tumor accumulation potential, remarkable biosafety, and biocompatibility. Combined, such synchronized delivery approach based on multi-responsive micelles might potentiate the efficacy of combination chemotherapy in clinical cancer treatment.
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INDUSTRIAL CROPS AND PRODUCTS
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2023
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IF 5.9
Shuzhen Yang,Min He,Dongmei Li,John Shi,Litao Peng,Liu Jinjing
The antifungal activity of essential oils is closely related to their components and contents. The study aimed to explore the structural activity of essential oil (EO) components against Diaporthe fusicola and modes of action. The results showed most of the tested compounds showed inhibitory effects on the growth of D. fusicola and carvacrol was the strongest one with half maximal effective concentration (EC 50 ) of 11.21 μL/L. The chemical structures of the tested compounds had great influences on their antifungal activity, in which the presence of CHO group, C C group or Ar-OH group in any EO components significantly increase the antifungal potency, but the increment of carbon chain length of saturated fatty aldehyde decreased antifungal activity. In order to analyze the antifungal action mode of EO components, the correlations between EC 50 and oxidation-reduction potential (ORP), cell-membrane permeability and respiration inhibition of the tested compounds were further evaluated. The results showed antifungal activities of these EO components seemed to be accomplished by increase of cell membrane permeability and inhibition of the respiration of D. fusicola . The levels of increased membrane permeability, inhibited respiration and ORP of chemicals were all significantly correlated with EC 50 . This results indicated the action mode of these EO components against D. fusicola may be associated with the increment of cell membrane permeability and respiration inhibition via disrupting redox homeostasis in fungal cells, and oxidation-reduction property could be used as a secondary indicator for screening the antifungal agent from EO components.
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Materials Science and Engineering B-Advanced Functional Solid-State Materials
·
2023
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IF 3.6
Yang Tao,Rufeng Zhu,Panpan Hao,Wei Jiang,Mufang Li,Qiongzhen Liu,Liyan Yang,Yuedan Wang,Dong Wang
The dual-mode organic electrochemical transistors (OECT) were successfully assembled on fabric. The property of different devices has been compared. In the depletion mode, the PEDOT: PSS/textile-based OECT exhibit high transconductance of 75 mS, and the switching ratio of 8 × 10 2 . In accumulation mode, the PEDOT: PSS/DETA/textile-based OECT exhibit transconductance of 0.55 mS, switching ratio can reach 31.7. Both modes have good cycle stability. A lactate (LA) sensor was prepared by modifying the gate electrode with lactate oxidase. The sensitivity of the sensor is 69.97 mV/decade and 47.8 mV/decade in the depletion and accumulation mode, respectively. The LA sensor in both modes has good linear response in a wide concentration range of 100 pM-10 mM. The sensor has been successfully applied to the detection of LA in sweat sample. It has the advantages of simple preparation, good performance, and convenient integration into clothes, which provides a feasible platform for intelligent wearable and portable medical detection.
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Frontiers in Bioengineering and Biotechnology
·
2023
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IF 5.7
Du Jinsong,Chen Guanping,Yuan Xinyi,Yuan Jiang,Li Li
Although the potential of metal-organic framework (MOF) nanoparticles as drug delivery systems (DDS) for cancer treatment has been established by numerous studies, their clinical applications are still limited due to relatively poor biocompatibility. We fabricated a multifunctional Cu-MOFs@Keratin DDS for loaded drug and chemodynamic therapy (CDT) against tumor cells. The Cu-MOFs core was prepared using a hydrothermal method, and then loaded with the anticancer drug DOX and wrapped in human hair keratin. The Cu-MOFs@Keratin was well characterized by transmission electron microscopy (TEM), fourier transform infrared spectroscopy (FTIR), dynamic light scattering (DLS), and X-ray photoelectron spectroscopy (XPS). Characterization and pharmacokinetic studies of Cu-MOFs@Keratin were performed in vitro and in vivo. The keratin shell reduced the cytotoxicity and potential leakage of Cu-MOFs to normal cells, and allowed the drug-loaded nanoparticles to accumulate in the tumor tissues through enhanced permeability and retention effect (EPR). The particles entered the tumor cells via endocytosis and disintegrated under the stimulation of intracellular environment, thereby releasing DOX in a controlled manner. In addition, the Cu-MOFs produced hydroxyl radicals (·OH) by consuming presence of high intracellular levels of glutathione (GSH) and H2O2, which decreased the viability of the tumor cells.
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高影响因子
JOURNAL OF MATERIALS SCIENCE
·
2023
·Vol. 58
·期 6
·2764-2781
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IF 4.5
Zhang Xiangke,Chen Ming,Kan Yuping,Dong Yumeng,Zhang Xinyu,Wang Xueying,Su Hui,Xu Shiyi,Yan Xueying
A series of antitumor therapies such as chemotherapy, photothermal therapy, and photodynamic therapy have certain limitations when applied alone. Herein, the chemotherapy drug doxorubicin (DOX) and photosensitizer new indocyanine green (IR820) were loaded into mesoporous silica nanoparticles (MSNs), and it was wrapped by manganese dioxide (MnO 2 ) on the surface. Optimization of the synthesis conditions by Box–Behnken design (BBD) generated DOX-IR820@MSN–MnO 2 nanoparticles with a high loading capacity. The experiments suggested that the nanocomposites had tumor microenvironment response characteristics and excellent photodynamic and photothermal properties. It can significantly regulate the level of glutathione and reduce the antioxidant effect of cells to enhance the photodynamic effect. NIR laser irradiation increases the temperature and accelerates drug release. The relative cell viability in the multi-modal combination therapy group almost disappeared at 17.20%. Combined tumor microenvironment response and multi-modal therapy show unique advantages in inhibiting tumor cell proliferation and have promising applications and development potential in cancer treatment. Graphical Abstract
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DESALINATION
·
2022
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IF 11.211
Zhuoran Yang,Dan Yang,Chunyu Yang,Kai Yu,Xiang Chen,Fengyu Qu,Wei Guo
Desalination using solar-driven water evaporation is one of the most efficient, green, and sustainable approaches. However, the purification of oil-contaminated seawater, salt inhibition, and recovery of water evaporators remain challenging. Herein, a water evaporator based on a C/ZnFe 2 O 4-x composite deposited on floral foam (FF) was developed, and its water evaporation performance was optimized by adjusting the reaction and calcination temperatures, concentration of deposited C/ZnFe 2 O 4-x , and height of the FF. Owing to the excellent optical properties of C/ZnFe 2 O 4-x , the water evaporator absorbed more than 96 % of sunlight in the wavelength range of 280–2500 nm, and its excellent magnetic properties promoted the recycling of the evaporator using a magnetic drive. The evaporator exhibited an excellent solar evaporation rate of 4.06 kg m −2 h −1 , and a high light-to-heat conversion efficiency of 95.4 % under one-sun irradiation. The water evaporator exhibited excellent salt inhibition properties, and no salt crystals formed during the water evaporation process. The total organic carbon and organic dissolved matter in the purified water were significantly lower than those of the oil-contaminated seawater. Because of its low cost, high evaporation rate , and excellent durability, recyclability , and salt inhibition properties, the 3D evaporator can be a reliable device for purifying oil-contaminated seawater.
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