Advanced Science
·
2025
·
IF 14.1
Yunzhi Liu,Cuicui Huang,Min Luo,Wenfu Lu,Baifeng Zhang,Lu Bai,Shuyue Zheng,Yanan Tan,Shanshan Li,Huali Wang,Lanqi Gong,Xinyuan Guan
Nasopharyngeal carcinoma (NPC) is one of the common head and neck cancers in Southern China and Southeast Asia. Although current studies have adequately characterized the tumor microenvironment (TME) of NPC, little attention has been paid to how cell-cell interactions within the TME promote tumorigenesis. In this study, it is found that C1q + tumor-associated macrophages (TAMs) are significantly enriched in NPC tumors. Moreover, both enriched C1q + TAMs and elevated C1q expression are associated with the progression and poor prognosis in NPC patients. In vitro and in vivo studies demonstrate that C1q directly boosts the malignancy and stemness of tumor cells. Mechanistically, C1q activates the Phosphatidylinositol-3-kinase (PI3K)/AKT pathway through interacting with GPR17, a member of the G protein-coupled receptor family, thereby inducing DNA hypermethylation of tumor cells to promote tumor development. It is further proved that DNA hypermethylated NPC cells induced by C1q elicited the immunosuppressive phenotype of TAMs. Targeted blockade of C1q with a neutralizing antibody restricts NPC progression in the humanized mouse model. It is assumed that the differentiation of C1q + TAMs possibly acquired both M1 and M2 polarization conditions. These findings provide new insights into the cellular communication in the TME of NPC and may have important applications for the development of new targeted therapies.
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高影响因子
MICROCHEMICAL JOURNAL
·
2025
·
IF 5.1
Shenghao Zhang,Meiling Zhang,Yinhao Zhang,Yingxia Zong,Xu Hun
Here, we designed a catalytic DNA assembly (CDA) amplification strategy to construct a photoelectrochemical (PEC) assay to detect the specific target F3L-gene of Monkeypox Virus (MPXV). Graphdiyne (GDY) and CuSe were synthesized by a surface-assisted deprotection/polymerization method and hydrothermal method, respectively. Then AuNPs and GDY/CuSe nanocomposites were modified on the surface of gold electrode (GE). The hairpin DNA4 (H4) was incubated onto the surface of the electrode by Au-S bond. When the target was present, the CDA was occurred by hairpin DNA1 (H1) and hairpin DNA2 (H2). Then, DNA3 (H3) hybridized with the single stranded region of H1-H2 complex, formed a site that can be hydrolyzed by exonuclease III, led to exonuclease III hydrolysis of DNA and to release the H3 primer (H3P). H3 further opened H4 and triggered a subsequent CDA reaction, ultimately formed a Y-shaped DNA structure on the electrode surface with the help of DNA5 (H5) and DNA6 (H6). The two end sequences of H4 and H6, which constituted the Y-shaped structure, were designed as split H3P fragments. They can be used as primers to trigger CDA reactions for more efficient and characteristic autocatalytic signal amplification. The Y-shaped structure carried H5-ALP, and catalyzed phosphonoxyphenol ( o -PP) to generate benzene-1,2-diol (CA), which could enhance the PEC signal. The linear range is 0.1 fM-10.0 pM with the detection limit of 0.05 fM (3S/N) for MPXV.
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高影响因子
BMC PLANT BIOLOGY
·
2025
·Vol. 25
·期 1
·1-19
·
IF 4.8
Zhang Xiaoxiao,Zhu Lili,Xu Zihan,Wang Zhimin,Dai Liping
Background DNA methylation plays a key role in regulating plant growth and development, secondary metabolism, and environmental adaptability and is affected by DNA methyltransferase (C5-MTase) and demethylase (dMTase). However, the response of C5-MTase and dMTase genes to abiotic stress in Isodon rubescens remains largely unknown. Results In this study, eight IrC5-MTase genes and five IrdMTase genes were identified within the genome of I. rubescens . The IrC5-MTases were phylogenetically divided into four subfamilies (CMT, DRM, MET and DNMT2), and the IrdMTase were divided into two subgroups (ROS and DML) on the basis sequence similarity, conserved motif and domain architecture. The IrC5-MTase and IrdMTase genes were unevenly distributed on 5 and 4 chromosomes, respectively. Cis‑acting element analysis revealed that the IrC5-MTase and IrdMTase genes were enriched with multiple light-, phytohormone- and stress-responsive elements. Importantly, the level of DNA methylation in I. rubescens was influenced by different 5-azacytidine (5-azaC) concentrations or light intensities but unaffected by different NaCl concentrations. Accordingly, the expression of IrC5-MTase and IrdMTase genes were significantly upregulated or downregulated in response to different concentrations of 5-azaC or amounts of light stress, however, no conspicuous differences were observed under different degrees of NaCl stress. Expression correlation analysis revealed that the expression of IrCMT2a , IrMET1 , and IrDML4 was most closely related to the DNA methylation level in I. rubescens . Conclusions Our results reveal the characteristics and differential expression of the IrC5-MTase and IrdMTase genes in response to 5-azaC, light or NaCl stresses and will facilitate future epigenetic studies in I. rubescens .
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高影响因子
ANALYST
·
2020
·Vol. 145
·期 5
·1933-1942
·
IF 3.978
Xiuyun Wang,Jie Zhang,Yuanan Wei,Tianyu Xing,Tingting Cao,Shuo Wu,Fenghui Zhu
A simple and easy-operation electrode modification strategy was proposed using Cu-MOF/GO nanohybrids for physiologists and pathologists for the feasible and reliable simultaneous electrochemical detections of DNA bases, namely guanine and adenine. The nanohybrids were prepared via a simple ultrasonic method and were employed for the fabrication of a sensing interface. SEM, TEM, XRD, FT-IR, and electrochemical characterizations were used to characterize the general morphology and structure of the nonohybrids. The proposed Cu-MOF/ERGO/GCE exhibited ultra-stable and high-sensitivity performance in the simultaneous electrochemical detection of guanine and adenine. The recorded DPV curves revealed a linear increase in the faradaic signals with increase in the concentrations of guanine and adenine in the range of 0.02–10 µM and 20–100 µM for guanine, and 0.005–20 µM and 40–200 µM for adenine. The relative standard deviation of guanine and adenine for 50 consecutive detections is 1.37% and 1.92%, respectively. It was proved that the proposed Cu-MOF/ERGO/GCE can be performed for the detection of guanine and adenine in real samples, such as Herring sperm DNA, and satisfactory results were obtained. This strategy does not require complicated modification procedures, professional modification techniques, or sophisticated instruments, but it can provide a highly sensitive and stable detection method, which is expected to expand and deepen the applications of electrochemical detection in life science research.
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International Journal of Nanomedicine
·
2023
·
IF 8
Run Chen,Pengchao Sun,Xiao Chu,Xiaorong Pu,Yang Yang,Nan Zhang,Yongxing Zhao
Background Aptamers have been widely used as targeted therapeutic agents due to its relatively small physical size, flexible structure, high specificity, and selectivity. Aptamers functionalized nanomaterials, not only enhance the targeting of nanomaterials, but can also improve the stability of the aptamers. We developed aptamer C2NP (Apt) conjugated straight DNA nanotubes (S-DNT-Apt) and twisted DNA nanotubes (T-DNT-Apt) as nanocarriers for doxorubicin (DOX).Methods The twisted DNA nanotubes (T-DNT) and straight DNA nanotubes (S-DNT) were assembled with a scaffold and hundreds of staples. Apt was site-specifically anchored on DNA nanotubes with either different spatial distribution (3 or 6 nm) or varied stoichiometry (15Apt or 30Apt). The developed nanocarriers were characterized with agarose gel electrophoresis and transmission electron microscopy. The drug loading and release in vitro were evaluated by measuring the fluorescence intensity of DOX using a microplate reader. The stability of DNT in cell culture medium plus 10% of FBS was evaluated by agarose gel electrophoresis. The cytotoxicity of DNA nanostructures against K299 cells was tested with a standard CCK8 method. Cellular uptake, cell apoptosis, cell cycle and reactive oxygen species level were investigated by flow cytometry. The expression of p53 was examined by Western Blot.Results T-DNT-30Apt-6 exhibited the highest cytotoxicity when the concentration of Apt was 120 nM. After intercalation of DOX, the cytotoxicity of DOX@T-DNT-30Apt-6 was further enhanced due to the combination of chemotherapy of DOX and biotherapy of Apt. The enhanced cytotoxicity of DOX@T-DNT-30Apt-6 can be explained by the increase in the cellular uptake, cell apoptosis and intracellular ROS levels. Additionally, the interaction between Apt and its receptor CD30 could upregulate the expression of p53.Conclusion These results demonstrate that both stoichiometry and spatial arrangement of Apt on T-DNT-Apt influence the anticancer activity. The developed twisted DNA nanotubes may be a solution for the synergistic treatment of cancer.
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JOURNAL OF MOLECULAR LIQUIDS
·
2020
·
IF 5.065
Rui Wang,Na Li,Xing Hu,Junhui Pan,Guowen Zhang,Xinan Zeng,Deming Gong
The binding characteristics of food antioxidant tert -butylhydroquinone (TBHQ) and its oxidation product tert -butylquinone (TBQ) with calf thymus DNA (ctDNA) in vitro were investigated using multispectroscopy, chemometrics , viscosity measurements, gel electrophoresis assays and molecular docking . Analysis of fluorescence spectra found that TBHQ and TBQ bound with ctDNA to form 1:1 complexes with the binding constant in the order of 10 4 L mol −1 . The concentration profiles and the pure spectra for the components (TBHQ, TBQ, ctDNA and TBHQ– or TBQ–ctDNA complex) were obtained through analyzing the expanded ultraviolet-visible absorption data matrix by multivariate curve resolution–alternating least squares algorithm, which successfully monitored the binding process of TBHQ/TBQ with ctDNA. Fluorescence displacement, viscosity and molecular docking experiments showed that TBHQ bound to ctDNA through intercalation binding, while TBQ bound to the minor groove of ctDNA. The binding of TBHQ to ctDNA induced the conversion of DNA conformation from B-form to A-form, while TBQ caused a minor perturbation on the DNA conformation. Gel electrophoresis results indicated that the combination of TBHQ and Cu(II) could induce DNA damage. This study may contribute to understanding the binding mechanisms of TBHQ and TBQ with ctDNA and further evaluate the potential toxicological effects of TBHQ as a food additive .
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TALANTA
·
2022
·
IF 6.556
Jialin Zhang,Wenjing Liu,Jinhui Li,Kangqiang Lu,Herui Wen,Jiali Ren
The foodborne pathogens have caused many life-threatening diseases. Herein, a new electrochemical platform was developed for detection, using 3D walking machine and enzyme-free toehold-mediated strand displacement. High-variable region of 16S rDNA fragment of Escherichia coli ( E. coli ) was used as the target to verify the approach. In presence of target, Exonuclease III (Exo III)-powered hairpin-loaded Au nanoparticles ( [email protected] ) was aroused, which emerged large amounts of 3D toehold-loaded AuNPs ( [email protected] ) walking machine. The “legs” of the walking machine hybridized with hairpin2 (HP2) tracks probe modified on surface of electrode and exposed toehold region of HP2 probe. The toehold-mediated strand displacement triggered rolling of AuNPs along the electrode, through the interaction of hairpin3-F c (HP3-F c ) probe with toehold region of HP2 probe. Thus large amounts of F c were closed to the electrode and led to significant current responses for E. coli detection. The method could continuously recycle low-concentration targets. Furthermore, due to the increase of “legs” and decrease of the derailment of leg DNA , which was beneficial to shorten detection time and amplify response. The detection limits (LOD) was 20 CFU/mL, the detection time was less than 1.5 h. This method was promising to be applied in early diagnosis of foodborne pathogen.
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高影响因子
ENVIRONMENT INTERNATIONAL
·
2020
·
IF 7.577
Hui Zhao,Misha Liu,Tao Jiang,Jinjin Xu,Huirong Zhang,Chaofan Yu,Zipeng Liu,Ying Wang,Longhua Tang
There are currently increasingly concerns over DNA damage related to free radicals due to their vital roles in human health, especially high-performance detection method. Herein, we report an ultra- sensitive monitoring of DNA damage associated with free radicals exposure using interdigitated electrode (IDE) array for the first time. The proposed IDE array was equipped with DNA-wrapped carbon nanotube-based bridges, which utilized the DNA damage mechanism due to the free radicals’ attack and the efficient electrical detection nature of the interdigitated electrode. Experiments have been performed, and the results showed the device’s capability for detecting DNA damage induced by multiple free radicals generated from different sources, including the Fenton reaction, UV radiation and cigarette smoke, showing the promising ability for DNA damage detection. In addition, the carbon nanotubes bridge-based interdigitated electrode sensor enabled different levels of sensing of DNA damage with great sensitivity and a wide detection range. It was illustrated that the ultrasensitive detection of free radicals generated from ultraviolet radiation (15 min – 125 min), cigarette smoke tar (1 μg/mL to 10 μg/mL) and Fenton reaction under different concentration of H 2 O 2 (2.5 pM – 100 pM), have been detected successfully. Typically, the IDE array supports further performance improvement for the electrochemical detection in an ultrasensitive and high throughput route.
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高影响因子
Scientific Reports
·
2017
·Vol. 7
·期 1
·1-7
·
IF 4.259
Cao Hongmei,Fang Xueen,Liu Peng,Li Hua,Chen Weiwei,Liu Baohong,Kong Jilie
Determination of proteins, especially low-abundance proteins with high sensitivity and specificity, is essential for characterizing proteomes and studying their biochemical functions. Herein, a novel Magnetic-Immuno-Loop-Mediated Isothermal Amplification (Im-LAMP) based on DNA-encapsulating liposomes (liposome-Im- LAMP), was developed for trace amounts of proteins. To the best of our knowledge, this is our first report about the magnetic Im-LAMP approach based on liposomes encapsulated template DNA as the detection reagent. The DNA template was released from liposomes and then initiated an Im-LAMP reaction, generating the fluorescence signal with high sensitivity and rapidity. This technique was applied for the determination of P-glycoprotein as a model protein. It was demonstrated that the technique exhibited a dynamic response to P-glycoprotein ranging from 1.6*10 −2 to 160 pg/ml with a greatly low detection limit of 5*10 −3 pg/ml (5 fg/ml) which is substantially better than conventional enzyme-linked immunosorbent assays (ELISA). This ultra sensitivity was attributed to the LAMP reaction initiated by the enormous DNA targets encapsulated in liposomes. This magnetic liposome-Im–LAMP as an alternative approach is attractive for applications in other low-abundance proteins detection in clinical diagnostics.
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高影响因子
RSC Advances
·
2022
·Vol. 12
·期 46
·30024-30029
·
IF 4.036
Baozhu Zhang,Ziyao Yang,Yuxia Li,Ling Ma,Fenfang Li,Xiuqing Lv,Guangming Wen
A label-free aptasensor has been fabricated in order to detect adenosine triphosphate (ATP) using turn-on fluorescence DNA-Ag NCs. The fluorescence of the DNA-Ag NCs could increase remarkably with the addition of ATP mainly because ATP specifically interacts with its aptamer to change the microenvironment of the darkish DNA-Ag NCs located at one terminus or two termini due to the conformational alteration of the aptamer structure. The proposed sensor can detect ATP in a linear range of 6–27 mM with a good detection limit of 5.0 μM. Additionally, the proposed method succeeded in detecting ATP in fetal bovine serum.
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高影响因子
MICROCHIMICA ACTA
·
2020
·Vol. 187
·期 12
·1-8
·
IF 6.232
Pang Jiawei,Lu Yuexiang,Gao Xinyu,He Liuying,Sun Jingwei,Yang Fengyi,Liu Yueying
A fluorescence method for the determination of inorganic pyrophosphatase (PPase) activity has been established based on copper nanoclusters (CuNCs). The polythymine of 40 mer (T40) acts as a template for the reduction reaction from Cu 2+ to Cu 0 by ascorbic acid (AA). This reaction leads to the formation of fluorescent CuNCs with excitation/emission peaks at 340/640 nm. However, the higher binding affinity between inorganic pyrophosphate (PPi) and Cu 2+ hinders the effective formation of CuNCs. This shows low fluorescence intensity. PPase catalyzes the hydrolysis of PPi into Pi during which free Cu 2+ ions are produced. This facilitates the formation of fluorescent CuNCs. Thus, the fluorescence intensity was restored. The fluorescence enhancement of the system has a linear relationship with PPase activity in the range 0.3 to 20 mU·mL −1 , and the detection limit is0.2 mU·mL −1 . The relative intensity (I/I 0 ) at 640 nm for the analytical solution versus system is also employed to screen the inhibitor for PPase with high efficiency.
Schematic representation of a fluorescent assay for the determination of inorganic pyrophosphatase activity and screening its inhibitor based on single-strand polythymine-scaffolded copper nanoclusters.
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ANALYTICA CHIMICA ACTA
·
2011
·
IF 4.311
Lizhu Yang,Ying Xu,Xiuhua Wang,Jing Zhu,Renyi Zhang,Pingang He,Yuzhi Fang
We functionalized aligned carbon nanotubes (ACNTs) electrode with a new kind of β-cyclodextrin (β-CD) derivative through diazotization reaction. The resulting β-CD/ACNTs electrode was used to detect DNA hybridization in homogeneous solution based on host–guest recognition technology . In the sensing protocol, one special DNA probe was designed with a stem-loop structure and both ends modified, which we called dually labeled DNA probe (DLP). One end of the DLP was labeled with dabcyl as guest molecule for β-CD/ACNTs electrode capture, and the other end was labeled with a CdS nanoparticle as an electrochemical tag to indicate the occurrence of DNA hybridization. In the absence of the target DNA sequence , the DLP maintains its hairpin structure in solution phase and would not be captured and detected by the β-CD/ACNTs electrode. In the presence of the complementary target sequence, the conformational structure of the DLP was altered and a double-stranded DNA (dsDNA) molecule was formed by the hybridization of DLP and complementary DNA sequence. Consequently, the dsDNA was captured by the β-CD/ACNTs electrode owing to guest–host recognition between β-CD and dabcyl. The electrochemical signal from the CdS nanoparticle–dsDNA/β-CD/ACNTs was then measured. Under optimized detection conditions, the proposed method showed high sensitivity and selectivity with a detection limit of 5.0 × 10 −13 M for complementary DNA sequence.
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