MICROCHIMICA ACTA
·
2017
·Vol. 184
·期 10
·3833-3840
·
IF 4.58
Ma Liling,Sun Shan,Wang Yuhui,Jiang Kai,Zhu Jiali,Li Jun,Lin Hengwei
The authors describe a fluorometric nanoprobe for hypochlorite ion. It was prepared by covalently linking o-phenylenediamine to graphene quantum dots (GQDs). The probe displays green fluorescence with excitation/emission peaks at 440/534 nm. In the presence of chlorite, oPD is oxidized to form a quinone-iminium compound which induces a reduction in fluorescence intensity. The nanoprobe enables chlorite to be determined with a 69 nM limit of detection. The probe is highly selective, well water soluble and biocompatible. It was successfully employed in the determination of chlorite in spiked tap water and living cells. The method is believed to represent a promising tool for the determination of chlorite in environmental, biological and clinical samples.
A photoinduced electron transfer (PET) fluorimetric probe for chlorite detection based on o-phenylenediamine (oPD) modified graphene quantum dots (GQDs). It was applied to imaging of chlorite in MCF-7 cells.
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ANALYTICAL LETTERS
·
2017
·
IF 1.15
Lingzhi Zhao,Zhao Li,Liu Zhao,Chenxiao Zhang
The increasing number of patients suffering from tinnitus may be explained by an imbalance in the antioxidant defense system and reactive oxygen species formation, suggesting the importance of the redox homeostasis in this condition. Endogenous biothiols play important roles in maintaining redox homeostasis. Hence, to understand the role of biothiols in the pathological process of tinnitus, this study demonstrates an in vivo method for monitoring the concentrations of biothiols in the paraflocculus of the rat cerebellum during tinnitus induced by the injection of salicylate. Resorufin-based fluorescent probe 1 was used as the selective probe with in vivo microdialysis. Probe 1 was premixed with microdialysates from the paraflocculus of the rat cerebellum and transferred into a cuvette for continuous-flow fluorescence. A linear relationship between the fluorescence and the concentrations of cysteine, homocysteine, and glutathione was from 1 to 15 µM, with detection limits of 128, 148, and 183 nM, respectively. The basal level of total reduced biothiols in the paraflocculus of the rat cerebellum microdialysate was determined to be 10.62 ± 1.34 µM based on the calibration curve for homocysteine. The injection of sodium salicylate into the animals significantly decreased the reduced biothiol concentrations in the paraflocculus of the rat cerebellum from 60 min, reaching 39.5 ± 6.20% of the basal level. In contrast, at 120, 180, and 300 min, the concentrations of reduced biothiols were 55.73 ± 9.35, 58.26 ± 9.56, and 91.69 ± 6.91% of the basal level, respectively. These results imply that the decrease in reduced biothiols in the paraflocculus of the rat cerebellum may be associated with salicylate-induced tinnitus. This study offers a new platform for in vivo monitoring of reduced biothiols in the paraflocculus of the rat cerebellum following salicylate-induced tinnitus and may be useful for the investigation of this condition.
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INDUSTRIAL CROPS AND PRODUCTS
·
2016
·
IF 3.449
Xin Wang,Minglong Wang,Jianguo Cao,Yuhuan Wu,Jianbo Xiao,Quanxi Wang
Flavonoid extracts of fern are conductive to phylogeny and genesiology as well as pharmacological activities. Ferns are widely distributed in Tianmu Mountain. Flavonoid concentrations and antioxidant activity in extracts of 49 ferns from Tianmu Mountain were investigated and analyzed. The result showed that the total flavonoid contents of 49 ferns ranged from 3.3 to 191.7 mg/g (w/w). Total flavonoid contents and antioxidant activity of fern allies were lower than those of filicinae. Woodwardia japonica a contained the highest flavonoid content (191.7 mg/g). The results also showed that most of ferns from Tianmu Mountain had strong antioxidant activity. The extracts from A. rhomboidea showed the highest DPPH scavenging activity, while extracts from W. japonica a showed the highest ABTS scavenging activity. The flavonoid contents of ferns are affected by ecological factors. In general, ferns living under dimmer sunshine have higher flavonoid contents and stronger ABTS scavenging activity than those living under strong sunshine. The ferns grown in trees, high altitude and high latitude contain higher flavonoid concentrations, whereas the ferns grown in soil, high altitude contain higher antioxidant activity.
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ANALYTICAL AND BIOANALYTICAL CHEMISTRY
·
2016
·Vol. 409
·期 1
·161-168
·
IF 3.125
Song Yonggui,Su Dan,Shen Yuan,Liu Hongyu,Wang Li
A novel open circuit potential biosensor (OCPS) composed of a working electrode and a Ag/AgCl reference electrode was designed for in vivo continuous glucose monitoring in this work. The macroporous carbon derived from kenaf stem (KSC) was used to construct a KSC microelectrode (denoted as KSCME) which was subsequently used to load glucose oxidase (GOD) as the working electrode. The resulting GOD/KSCMEs could catalyze the oxidation of glucose directly to result in changes of the open circuit potential ( V
oc ) of the OCPS. The V
oc of OCPS was dependent on the glucose concentration, showing a linear range of 0.03–10.0 mM ( R = 0.999) with a detection limit of 10 μM. In addition, the OCPS exhibited good selectivity for glucose over other common endogenous interferences. The feasibility of the proposed OCPS for glucose detection in mice skin tumors and normal tissue homogenate samples (in vitro experiment) and rat subcutaneous glucose monitoring (in vivo experiment) was also demonstrated with satisfactory results. The biosensor represents a novel example of a superficial cancer diagnostic device, and the proposed OCPS also provides new ideas for the development of a simple and highly selective device for continuous glucose sensing.
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高影响因子
Journal of Materials Chemistry B
·
2013
·Vol. 1
·期 11
·1607-1613
·
IF 0
Zhongde Mu,Xiangwei Zhao,Zhuoying Xie,Yuanjin Zhao,Qifeng Zhong,Ling Bo,Zhongze Gu
Recently, surface enhanced Raman spectroscopy (SERS) has been widely studied for chemical and biological sensing. SERS substrates combined with photonic architectures have attracted more and more attention. Photonic architectures in butterfly wings are too complicated to fabricate with either “top-down” or “bottom-up” approaches. Herein, we developed a simple, reproducible, inexpensive and green method to fabricate SERS substrates from different butterfly wings where the component chitosan/chitin was utilized as in situ reducer to synthesize gold nanoparticles in natural 3D photonic architectures. The SERS performances of three butterfly wings are compared. And the results show that a SERS substrate based on M. menelaus is better than the other three substrates, which could detect 10−9 M 4-ATP and has the lowest RSD and moderate SNR. In situ synthesis of AuNPs in butterfly wings with photonic architectures paves the way for fabricating multiple SERS substrates based on natural materials.
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Minerals
·
2015
·Vol. 5
·期 4
·654-664
·
IF 1
Wei Ge,Hongqiang Li,Yanzeng Ren,Feiyu Zhao,Shaoxian Song
The hydrophilic flocculation of pyrite fines in aqueous suspensions with corn starch was studied by measuring particle size distribution, microscopy observation and micro-flotation. Furthermore, the interaction of corn starch with pyrite was investigated by determining the adsorption density and based on zeta potential measurements and X-ray photoelectron spectrometer (XPS) analysis in this work. The results of the particle size distribution measurement show that corn starch can effectively aggregate pyrite fines, and the pyrite floccules (flocs) are sensitive to mechanical stirring. The micro-flotation results suggest that the mechanical entrainment of pyrite fines in flotation can be effectively eliminated through the formation of large-size flocs. The zeta potential of pyrite particles decreases with the addition of corn starch. The XPS results prove that carboxyl groups are generated on the digested corn starch, and both iron hydroxyl compounds and ferrous disulfide on the pyrite surface can chemically interact with the corn starch digested by sodium hydroxide.Keywords:hydrophilic flocculation;pyrite fines;corn starch;mechanical entrainment;adsorption
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RSC Advances
·
2015
·Vol. 5
·期 86
·70101-70108
·
IF 3.84
Qiong Wu,Jun Shi,Jing Wei,Liu Yang,Shaokui Cao
In this work, aliphatic poly(urethane-amine) (PUA) grafted mesoporous hollow hydroxyapatite (H-HAP) microparticles were prepared via in situ polymerization in supercritical CO2. Thermal-responsive PUA acted as the on–off gates inside the mesoporous H-HAP due to the stretch and shrinkage of the PUA polymer chains at different temperatures. The PUA-grafted hollow HAP (PUA-g-H-HAP) microparticles displayed high specific surface area (95 m2 g−1) and drug loading efficiency (60%). The in vitro drug release studies indicated that PUA-g-H-HAP microparticles exhibited distinguishable pH- and thermal-dependent drug release properties, and PUA on–off switches enabled the DOX release in a reversible way by simply adjusting the environmental temperature. Moreover, compared to the hollow HAP microparticles having a higher amount of released DOX over the initial 2 h (about 24.4% of total released drug over 24 h) at 37 °C and pH 7.4, PUA-g-H-HAP microparticles displayed a sustained release property with the value of only 10.7% deriving from the blockage of the stretched PUA chains inside the mesoporous H-HAP.
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JOURNAL OF CHROMATOGRAPHY A
·
2014
·
IF 4.258
Qian Wu,Dapeng Wu,Yafeng Guan
For in vivo extraction of trace and polar components in tissues, extraction materials should be biocompatible, highly permeable to matrix and have high extraction capacity for polar and ionic compounds. In this study, nanofibers (∼200 nm) with rough polyaniline (PAn) sheath were prepared successfully for the first time by coaxial electrospinning and coelectrospun polymer sacrificing method. The nanofibers (0.5 mg) were then compressed into tiny bar ( Φ 1 mm × 2 mm) and inserted directly into the aloe leaf for in vivo extraction of trace phytohormones. Due to the large surface area (13.97 m 2 /g) of nanofibers and large through pores formed between fibers, high extraction capacity (up to 10 μg/bar) and permeability to tissue matrix of the tiny bar can be obtained. Moreover, PAn sheath has high adsorption equilibrium constants (10 7 –10 8 mL/mol) for acidic phytohormones in aloe leaf. Four phytohormones were detected by in vivo extraction with a spatial resolution of 3–8 mm 3 , temporal resolution of 20 min, and limit of detection down to 60 pg/g. The quantification results were well met with that by traditional in vitro organic solvent extraction.
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International Journal of Inflammation
·
2014
·
IF 0
Hang Yang,Haili Qi,Jingli Ren,Jing Cui,Zhenfeng Li,Helge L. Waldum,Guanglin Cui
Nuclear factor-kappaB (NF-κB)/interleukin (IL-6) pathway links chronic inflammation to colitis associated cancer (CAC). In this study, we examined the dynamic temporal changes of the NF-κB/IL-6 pathway during the procession of experimental CAC mouse model. Mice were sacrificed after induction for 14, 16, 18, and 22 weeks for the examination of tumor burden, inflammation degree, and protein level of NF-κB and IL-6 in bowel tissues. The results showed that tumor burden and inflammation severity in the bowels were gradually increased over the observed time-points. The expressions of IL-6 and NF-κB proteins were gradually increased after induction of dysplastic lesions over times. These data provide new information on the dynamic temporal changes of NF-κB/IL-6 pathway in relation to CAC development that may be relevant in the design of future investigations of therapeutic interventions to effectively target CAC processes.
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JOURNAL OF NANOPARTICLE RESEARCH
·
2014
·Vol. 16
·期 7
·1-14
·
IF 2.278
Ma Xiaona,Shu Chang,Guo Jing,Pang Lili,Su Lin,Fu Degang,Zhong Wenying
Abstract A new targeted drug delivery system (DDS) based on oxidized single-wall carbon nanohorns (oxSWCNHs) was developed. Sodium alginate (SA) was used to modify oxSWCNHs to improve its dispersibility and biocompatibility, the first time such a modification to oxSWCNHs was reported. The humanized anti-vascular endothelial growth factor (anti-VEGF) monoclonal antibody was bound to the SA as targeting group to selectively kill the tumor cells. Doxorubicin hydrochloride (DOX) was conjugated to oxSWCNHs in basic pH solution by π–π stacking, and its release was triggered by the lower pH as the micro-environment of the tumor. Quantitative analyses showed that the DOX@oxSWCNHs/SA complexes contained 1 g DOX per gram of oxSWCNHs. Cell experiment showed that the DOX@oxSWCNHs/SA-mAb effectively targeted the human breast adenocarcinoma (MCF-7) cells and rarely adhered to the human embryonic kidney 293 (HEK293) cells. And the anticancer effects of the complexes were higher than those of the free DOX. Pharmaceutical efficiency in vivo showed that the relative tumor volumes (RTV) of normal saline (NS) group, oxSWCNH/SA-mAb (2.5 mg/kg) group, DOX (2.5 mg/kg) group, and DOX@oxSWCNHs/SA-mAb (2.5 mg/kg) group were approximately 61, 56, 14, and 7.2, respectively. In addition, higher drug dose (5 mg/kg) of DOX@oxSWCNHs/SA-mAb resulted in a better antitumor activity. Histopathological studies in mice confirmed that the DOX@oxSWCNHs/SA-mAb complexes did not demonstrate any detectable hepatotoxicity, cardiotoxicity, and nephrotoxicity. Graphical abstract
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ACS Nano
·
2014
·
IF 12.033
Enguo Ju,Zhen Liu,Yingda Du,Yu Tao,Jinsong Ren,Xiaogang Qu
Probes for detecting highly reactive oxygen species (hROS) are critical to both understanding the etiology of the disease and optimizing therapeutic interventions. However, problems such as low stability due to autoxidation and photobleaching and unsuitability for biological application in vitro and in vivo, as well as the high cost and complex procedure in synthesis and modification, largely limit their application. In this work, binary heterogeneous nanocomplexes (termed as C-dots-AuNC) constructed from gold clusters and carbon dots were reported. The fabrication takes full advantages of the inherent active groups on the surface of the nanoparticles to avoid tedious modification and chemical synthetic processes. Additionally, the assembly endowed C-dots-AuNC with improved performance such as the fluorescence enhancement of AuNCs and stability of C-dots to hROS. Moreover, the dual-emission property allows sensitive imaging and monitoring of the hROS signaling in living cells with high contrast. Importantly, with high physiological stability and excellent biocompatibility, C-dots-AuNC allows for the detection of hROS in the model of local ear inflammation.
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高影响因子
BIOMATERIALS
·
2014
·
IF 8.312
Zhaowei Chen,Zhen Liu,Zhenhua Li,Enguo Ju,Nan Gao,Li Zhou,Jinsong Ren,Xiaogang Qu
Over-generation of reactive oxygen species (ROS) is closely associated with the biological processes of rheumatoid arthritis (RA). Thus, efficient monitoring ROS in inflammatory joints would be essential for better understanding the pathogenesis and optimizing therapeutic interventions. Herein, we designed a ratiometric nanoprobe utilizing upconversion nanoparticles (UCNPs) conjugated with chromophore labeled hyaluronic acid (HA) for high sensitively sensing ROS in the aqueous solution, bioimaging ROS in inflammatory mimic cells and diagnosing RA in vivo. In this approach, the conjugation of HA conferred UCNPs not only water solubility but also biocompatibility and ROS recognizing properties. Particularly, the HA backbone cleavage and detachment of chromophore labeled HA fragments from UCNPs induced by ROS inhibited the luminescent energy transfer (LRET) and allowed rational metric upconversion luminescence (UCL) emission as the detection signal. Importantly, the upconversion nanoprobe showed high effectiveness for early assessing the treatment response of arthritic animals to an antiarthritic drug-methotrexate (MTX).
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