INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2013
·
IF 2.596
Xiaofen Xu,Yanggong Li,Yuanyuan Shen,Shengrong Guo
Norcantharidin-conjugated chitosan conjugates (NCTD-CSs) with different degrees of substitution ( DS , 60.2% and 97.9%) were synthesized and characterized by 1 H NMR, FT-IR, and XRD. In comparison with CS, the NCTD-CSs had lower crystallinity and better water solubility. Less than 6% NCTD was released from the NCTD-CSs through the hydrolysis of ester bonds in phosphate-buffered solution (PBS, pH 5.0 and 7.4) within 16 days, showing sustained drug release characteristic. The result of cytoxicity study showed that the NCTD-CSs were cytotoxic to MGC80-3 cells and their cytotoxicity was lower than that of NCTD. Fluorescence microscope and flow cytometry analysis demonstrated that the amount of the NCTD-CSs uptaken by MGC80-3 cells increased as incubation time. Confocal microscopy study showed that the NCTD-CSs were internalized into cells by endocytosis and mainly localized in lysosomes within 24 h. It was found that the NCTD-CSs arrested MGC80-3 cell cycle at G2/M phase and induced cell death via cell apoptosis similarly to NCTD. These results indicated that the NCTD-CSs might be an efficient NCTD delivery system for cancer therapy.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
RSC Advances
·
2013
·Vol. 3
·期 45
·23423-23431
·
IF 2.562
Qin Wang,Junping Zhang,Aiqin Wang
A series of magnetic chitosan/Fe3O4/halloysite nanotubes/ofloxacin (CTS/Fe3O4/HNTs/OFL) microspheres was prepared by spray-drying for the sustained release of OFL. CTS was in situ crosslinked by glutaraldehyde in the spray-drying process. The magnetic microspheres were characterized by FT-IR, scanning electron microscopy and thermogravimetric analysis. The magnetic properties, encapsulation efficiency, drug loading and in vitro sustained release properties of the magnetic microspheres for OFL were also evaluated. The CTS/Fe3O4/HNTs/OFL microspheres show superparamagnetic properties and fast magnetic response. The Fe3O4 nanoparticles have a remarkable effect on the surface morphology of the microspheres compared with HNTs. The encapsulation efficiency and the cumulative release are closely related to HNTs and Fe3O4 contents, crosslinking density and CTS concentration. OFL is released from the magnetic CTS/Fe3O4/HNTs/OFL microspheres via anomalous transport.
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF POWER SOURCES
·
2013
·
IF 4.675
Lu Yue,Lingzhi Zhang,Haoxiang Zhong
Carboxymethyl chitosan (C-chitosan) is investigated as a new water soluble binder for Si anode of Li-ion batteries. The Fourier transformation infrared spectroscopy (FTIR) and X-ray photoelectron spectroscopy (XPS) measurements reveal that the strong hydrogen bonding is formed between the hydroxylated Si surface and the polar groups (–OH, –COOH and –NH 2 ) of C-chitosan. The Si/C-chitosan anode (Si:carbon black:C-chitosan = 62:30:8 in weight ratio) exhibits a high first discharge capacity (4270 mAh g −1 ) with a first coulombic efficiency of 89%, and maintains a capacity of 950 mAh g −1 at the current density of 500 mA g −1 over 50 cycles.
✓ 已验证
产品关联
DOI
高影响因子
Journal of Nanomaterials
·
2015
·
IF 1.644
Xiaobin Fang,Yingqi Xu,Jinming Zhang,Xianghong Lu,Yitao Wang,Meiwan Chen
A novel amphiphilic derivative of chitosan, namely, a linoleic acid-g-quaternary chitosan (LA-g-QC), was designed and synthesized as low toxic material for biomedical applications in this study. The chemical structure of LA-g-QC was characterized by Fourier transform infrared spectroscopy (FTIR), 1H nuclear magnetic resonance (1H-NMR), and elemental analysis. LA-g-QC could form nanosized micelles with self-assembly, which was confirmed by the results of critical micelle concentration (CMC) via fluorescence spectroscopy. The average size of LA-g-QC was 140 nm and its zeta potential was approximately +35.50 mV. CMC value was 31.00 mg/mL. Furthermore, LA-g-QC micelles, at final concentrations between 0.94 μg/mL and 30 μg/mL, did not inhibit the proliferation of HepG2 or SMMC 7721 cell lines. Taken together, LA-g-QC has low cytotoxicity and high potential for the preparation of novel drug-delivery micelles.
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF BIOMATERIALS SCIENCE-POLYMER EDITION
·
2013
·
IF 1.7
Yiyi Wang,Peifeng Liu,Jing Du,Ying Sun,Fenghua Li,Yourong Duan
This study aims to determine the specificity of anti-human epidermal growth factor receptor antibody (anti-HER2) modified monomethoxy polyethylene glycol-chitosan (mPEG-CS) nanoparticles (anti-HER2/mPEG-CS NPs) in delivering small interfering RNA (siRNA) to the human epidermal growth factor receptor 2 (HER2) positive cancer cells. Physicochemical properties of the siRNA-loaded anti-HER2/mPEG-CS NPs (anti-HER2/mPEG-CS-siRNA NPs), including size, surface charge, siRNA encapsulation efficiency, and in vitro release profile of siRNA from NPs, were characterized by particle size and zeta potential analyzer, and ultraviolet–visible spectrophotometer. MTT assay was used to study the in vitro cytotoxicity of the NPs. Fluorescent microscope and flow cytometer analysis results showed that anti-HER2/mPEG-CS-siRNA NPs had much efficient delivery of siRNA than the siRNA alone, Lipofectamine-siRNA complexes and mPEG-CS-siRNA NPs. These results demonstrated that anti-HER2/mPEG-CS-siRNA NPs had great potential applications as a targeted strategy for siRNA delivery.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
RSC Advances
·
2015
·Vol. 5
·期 69
·55640-55647
·
IF 3.84
Zhi-Sen Shen,Xiang Cui,Rui-Xia Hou,Qun Li,Hong-Xia Deng,Jun Fu
Tough porous chitosan–gelatin (CG) hydrogels have been prepared through in situ precipitation with tunable porosity and degradation rates by adjusting the preparation formulations. Compression tests showed a Young's modulus of 3.25 MPa and a compressive strength of 2.15 MPa for the optimized C4G4 hydrogels, which are similar to or higher than those for human cartilage. Cyclic compression tests showed overlapping hysteresis loops for several cycles, with a compressive toughness of about 75.8 J m−2. In vitro enzymatic degradation results showed a degradation of 65.9% in 70 days, comparable to the regeneration rate of engineered cartilage reported in literature. Moreover, in vitro cell culture experiments showed excellent adhesion and proliferation of human thyroid cartilage cells on these hydrogels, where the chondrocytes function to secrete ECM. These tough and biodegradable hydrogels may have potential applications for cartilage tissue engineering.
✓ 已验证
产品关联
DOI
高影响因子
ANALYTICAL LETTERS
·
2015
·
IF 1.03
Wen Ding,Min Wu,Meiling Liang,Henmei Ni,Ying Li
A novel electrochemically-based biosensor was developed for the determination of hydrazine by modifying a glassy carbon electrode with an aqueous dispersion of carboxylic group-functionalized carbon nanofiber/chitosan solution, and then absorbing hemoglobin on the surface of chitosan-carbon nanofibers. Nafion was used to coat the hemoglobin membrane. The interactions of hemoglobin and the nafion/chitosan-carbon nanofibers were investigated by ultraviolet-visible absorption, infrared, and circular dichroism spectroscopies. The results indicated that the native structure of hemoglobin was retained post-immobilization. The circular dichroism results showed that the α-helical structure of hemoglobin was preserved though a small change was observed in the presence of the nafion/chitosan-carbon nanofibers. The modified nanofibers were further characterized by scanning electron microscopy, electron impendence spectroscopy, and cyclic voltammetry. The electrocatalytic mechanism of hemoglobin to the oxidation of hydrazine was investigated and an irreversible diffusion-controlled electrode process was obtained. The electron transfer rate constant (ks), transfer coefficient (α), and Michaelis–Menten constant (Km) were also evaluated. The peak current of the catalytic oxidation was linear with hydrazine concentration from 3.722 × 10−5 to 1.601 × 10−3 molar with a correlation coefficient of 0.995. The detection limit was estimated to be 2.7 micromoles per liter. The sensitivity, stability, and reproducibility of the nafion/hemoglobin/chitosan-carbon nanofiber/glassy carbon electrode for the oxidation of hydrazine were also investigated.
✓ 已验证
产品关联
DOI
高影响因子
Marine Drugs
·
2017
·Vol. 15
·期 9
·284
·
IF 3.503
Wenbo Dong,Weiping Li,Yu Chen,Yanchun Ye,Shaohua Jin
In the present work, a fluorescent H2O2biosensor was constructed by encapsulating fluorescent probe Rhodamine B (RhmB) in the hydrophobic cavity of the cyclodextrin (β-CD) and immobilizing catalase (CAT) on the 2-NH2of chitosan (CTS) in a chitosan 6-OH immobilized β-cyclodextrin derivative (CTS-6-CD). The inclusion complex of CTS-6-CD to RhmB (CTS-6-CD-RhmB) was prepared by a solution method. Its structure and inclusion efficiency were determined by Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), and fluorescence spectroscopy (FL). CAT was immobilized on CTS-6-CD-RhmB to eventually form the functional membrane, CTS-6-CD-RhmB-CAT, via glutaraldehyde crosslinking, which was further characterized by FTIR and FL, and used as a H2O2biosensor. The functional membrane was used to simultaneously oxidize and detect H2O2. The detection condition was optimized as pH 8, a reaction temperature of 25 °C, and an immobilized enzyme concentration of 2 × 10−4mol/L. The fluorescence response of the biosensor exhibited a good linear relationship with the concentration of H2O2in the range of 20 mΜ–300 μM and the detection limit of 10−8mol/L.Keywords:chitosan;catalase;β-cyclodextrin;Rhodamine B;fluorescent biosensor
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Journal of Materials Chemistry B
·
2016
·Vol. 4
·期 19
·3387-3397
·
IF 4.872
Min Zhang,Jia Liu,Ying Kuang,Qilin Li,Hongyu Chen,Haifeng Ye,Li Guo,Yanglin Xu,Xueqin Chen,Cao Li,Bingbing Jiang
Suitable protection strategies utilized in anticancer drug delivery systems enable carriers to reach their targeted positions and release drugs intracellularly more effectively. In this study, a novel “stealthy” chitosan (CHI)/mesoporous silica nanoparticle (MSN) based complex system, named DOX@MSN-SS-CHI-PEG, was developed for tumor-triggered intracellular drug release. CHI was applied to block the pores of MSNs to prevent premature drug release, whereas mPEG was grafted on the surface of the nanoparticles via a pH-sensitive benzoic imine linker to protect the carriers. As the pH of solid tumor tissues is slightly lower than that of normal tissues, mPEG could leave the nanoparticles to expose positively charged CHI at the surface, which enabled the nanoparticles to enter cancer cells more easily. The MSNs were covered by CHI via redox-sensitive disulfide bonds. As a result, the carriers could release the drug intercellularly to kill cancer cells owing to the high concentration of glutathione (GSH) in the cytosol. In vitro drug release studies at different GSH concentrations proved the redox-sensitivity of DOX@MSN-SS-CHI-PEG. mPEG leaving studies demonstrated that mPEG could leave the nanoparticles effectively at pH 6.0. The cytotoxicity and cell internalization behavior were also investigated in detail. In conclusion, the novel DOX@MSN-SS-CHI-PEG drug delivery system, which was “stealthy” in the physiological environment at pH 7.4 because of the protection of mPEG, was “activated” in weakly acidic tumor tissues to achieve tumor-triggered intracellular drug release; this system has great potential for cancer therapy.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF COLLOID AND INTERFACE SCIENCE
·
2017
·
IF 4.233
Peng Xu,Xuguang Liang,Nannan Chen,Jingen Tang,Wei Shao,Qinwei Gao,Zhaogang Teng
In the work, we have synthesized silver (Ag) nanoparticles deposited chitosan (CS) microcapsules with magnetic multiple Fe 3 O 4 cores (denoted as Fe 3 O 4 /CS-Ag) as efficient catalysts for the reduction of 4-nitrophenol. The Fe 3 O 4 /CS-Ag catalysts are prepared by coating hydrophobic Fe 3 O 4 nanoparticles with chitosan via a multiple emulsion-chemical crosslinking method and following in situ deposition of Ag nanoparticles onto the surfaces. The morphology and composition of the Fe 3 O 4 /CS-Ag microcapsules are characterized by Fourier transform infrared (FT-IR) spectra, scanning electron microscopy (SEM), energy dispersive X-ray spectroscopy (EDX), and X-ray photoelectron spectroscopy. Moreover, the catalytic activity of the Fe 3 O 4 /CS-Ag catalyst was investigated, which shows a conversion efficiency as high as 98% within 15 min for the reduction of p-nitrophenol to p-aminophenol. At the same time, the recovered Fe 3 O 4 /CS-Ag catalyst with an external magnet can retain good activity after 10 cycles. These results indicate that the multiple emulsion-chemical crosslinking method is an efficient and simple way for the preparation of magnetic separable chitosan microcapsules, and the fabricated Fe 3 O 4 /CS-Ag is a promising catalytic material with excellent catalytic activity and convenient recovery ability.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
CARBOHYDRATE POLYMERS
·
2017
·
IF 4.811
Kaihang Chen,Binzhao Guo,Jiwen Luo
It is of technical and economic importance to develop an effective cosmetic ingredient against skin aging through low-cost and facile production. This study developed a polymer/layered silicate nanocomposite as cosmetic ingredient against skin aging. Firstly, quaternized carboxymethyl chitosan (QCMC)/organic montmorillonite (OMMT) nanocomposite (QCOM) was fabricated via solution-induced intercalation. QCOM with different mass ratios of QCMC to OMMT were studied by various characterizations as well as moisture-adsorption and retention activities and UV-protection capacity. QCOM was then added into a cosmetic formulation to prepare a cosmetic cream. And the evaluation of its efficacy about skin care was conducted. The results showed that the optimal QCOM had better moisture-adsorption and retention behaviors than hyaluronic acid, and QCOM solution displayed good UV-protection ability. Furthermore, the QCOM-containing cosmetic cream meets the hygienic standard for cosmetics; it has negligible dermal irritation and a prominent moisture-retention efficacy on human stratum corneum.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
FOOD CONTROL
·
2017
·
IF 3.496
Chenjun Zhuang,Yongli Jiang,Yu Zhong,Yanyun Zhao,Yun Deng,Jin Yue,Danfeng Wang,Shunshan Jiao,Haiyan Gao,Hangjun Chen,Honglei Mu
Polyvinyl alcohol (PVA), sodium alginate (SA) and chitosan (CHI) are all biodegradable materials with excellent film-forming properties. This study prepared bilayer films with PVA-CHI film as the outer layer and SA film as the inner layer. Fuzzy comprehensive evaluation was used to study the effects of the SA film formula where nano-cellulose, surfactant, calcium chloride and anti-bacterial agents were added. The performance of the bilayer films was evaluated considering water solubility, water barrier, mechanical and antibacterial properties. And the effects of the coatings on qualities of shell eggs were investigated. The results demonstrated that the bilayer film possessed lower water vapor permeability (WVP) than the single-layer one, and water droplet was more difficult to spread on bilayer film. Increasing the content of SA significantly increased ( p ≤ 0.05 ) the mechanical properties of bilayer film, and the tensile strength (TS) increased 1.8 times when the SA content increased from 1 to 2%. Calcium chloride decreased the film solubility, and Tween 80 reduced the surface tension of the film-forming solution. Moreover, ethyl paraben sodium significantly increased the antibacterial activity of the bilayer film against E.coli and L . innocua , but had negative impact on TS and WVP. According to the orthogonal test L9 (3 4), the optimal formula of SA layer was: SA 1.25%, calcium chloride 0.75%, Tween 80 0.175%, and ethyl paraben sodium 0.125%. In addition, coatings reduced weight loss, and maintained Haugh unit and yolk index of shell eggs during 15 days of storage at 25 °C, especially bilayer coating.
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）