INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2025
·
IF 8.5
Wanqiu Huang,Mengdi Hao,Liangqiang Chen,Hao Shen,Shaoning Yu,Ying Han,Fan Yang
Rapid quantitative detection of β-amylase is important for food quality, production efficiency, and food safety. However, current detection methods are time-consuming with low sensitivity. Fast, sensitive, and simple detection of β-amylase remains a challenge in practice. In the present work, we constructed a colorimetric β-amylase assay based on cross-linked nanozyme. The assay utilized the interaction between β-amylase and its specific substrate, p -nitrophenyl-β-maltopentaoside (PNPG3), which cross-links with the FeCo-BDC-NH 2 metal-organic framework nanozyme. The detection mechanism is based on β-amylase hydrolyzing the crosslinking agent PNPG3 on the surface of FeCo-BDC-NH 2 , leading to enhanced peroxidase-mimicking biological activity of the nanozyme in free state. The detection limit was 2.77 U L −1 , with a broad linear range (5–160 U L −1 ) and remarkable specificity. In addition, the assay was used in fermentation samples to validate the analytical performance. This assay offers an accessible and efficient method of determining β-amylase activity in the actual production.
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PubMed
高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2025
·
IF 13.2
Shumin Zhang,Kexin Zou,Qingmin Chen,Jie Wei,Tianhui Jiao,Min Chen,Yi Xu,Quansheng Chen,Xiaomei Chen
Developing a fast, accurate, and simple detection method for food allergens is imperative to prevent food allergy. In this study, an on–off-on photoelectrochemical (PEC) aptasensor with dual-signal amplification was constructed based on a covalent organic frame (COF) film to detect β-lactoglobulin (BLG) and Ara h1. Specifically, a uniform and stable TFTA COF film was in situ grown as a PEC signifier on the surface of an indium tin oxide (ITO) electrode. BLG recognition triggered enzyme-assisted circular signal amplification to introduce non-conductive SiO 2 -modified DNA (S0) to the platform, effectively quenching the PEC signal for ultra-sensitive detection of BLG. Upon adding Ara h1, SiO 2 -DNA was released from the sensing platform to restore the PEC signal, and hemin was added to form a G-quadruplex/hemin compound for secondary signal amplification. Thus, sensitive detections of BLG and Ara h1 were achieved with low detection limits (18 ng/mL for BLG and 2.6 ng/mL for Ara h1) and wide linear ranges (0.05–5 µg/mL for BLG and 0.01–0.2 µg/mL for Ara h1). The dual-amplification and dual-target-responsive PEC aptasensor provides a new idea to develop PEC analysis methods for multi-targets in food and environmental monitoring.
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高影响因子
INTERNATIONAL JOURNAL OF PHARMACEUTICS
·
2009
·
IF 3.061
Jianyu Xin,Zhizhang Guo,Xingyu Chen,Wenfeng Jiang,Jianshu Li,Maolin Li
A series of branched cationic β-cyclodextrin polymers (CPβCDs) with designed chemical structures were synthesized from β-cyclodextrin (β-CD), epichlorohydrin (EP) and choline chloride (CC). Indomethacin (IDM), an anionic drug, was chosen as a model drug to evaluate the drug loading capacities of CPβCDs. The formation of IDM-CPβCD complex was confirmed by 1 H NMR and DSC . Phase solubility studies and Job plots indicated that CPβCDs can solubilize IDM up to 100 times of its intrinsic solubility in a 1:1 complexation form. Mechanism studies with the help of adamantane revealed that the effective complexation is a combination of inclusion complexation, charge interaction and hydrophobic interaction. In addition, IDM-CPβCDs loaded alginate hydrogels were prepared and obtained controllable release profile in dissolution tests. The tunable structures of CPβCDs make them promising drug carriers with superior drug loading capacities and controllable drug release abilities.
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PubMed
高影响因子
ANALYTICAL SCIENCES
·
2020
·Vol. 36
·期 6
·687-695
·
IF 2.049
Hui WANG,Yazhou SHUANG,Tianci ZHANG,Liang LI,Laisheng LI
A cholesterol mono-derivatized β-cyclodextrin was synthesized and bonded onto silica gel (SBA-15) to obtain a cholesterol mono-derivatized β-cyclodextrin-bonded stationary phase (CHCDP). The chemical structures of mono-derivatized β-cyclodextrin and CHCDP were characterized by infrared spectroscopy, mass spectrometry, elemental analysis and thermogravimetric analysis, correspondingly. Furthermore, the separation ability of CHCDP in terms of achiral compounds was systematically evaluated by separating benzene homologs, polycyclic aromatic hydrocarbons (PAHs) and some positional isomers. As a result, CHCDP completely separated five benzene homologs and nine PAHs within 30 min under the reversed-phase. In addition, the chiral chromatographic property of CHCDP was also evaluated by separating some racemic compounds including flavanones, triazoles, β-blockers, etc. The results showed that the CHCDP exhibited high enantioselectivities towards most of selected analytes. The enantioresolutions were in the range from 1.43 to 2.51 on CHCDP. Especially the resolutions of 2′-hydroxyflavanone, hexaconazole, Dns-serine and atenolol were as high as 1.94, 1.91, 2.15 and 1.57, respectively. Obviously, the CHCDP was a versatile stationary phase with chiral and achiral separation capabilities in multi-mode chromatography, which was related to the introduction of cholesterol to the port of cyclodextrin, enhancing the hydrophobic interaction of cyclodextrin with achiral compounds, while maintaining the inclusion complexation of it with chiral compounds as well.
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高影响因子
JOURNAL OF MOLECULAR STRUCTURE
·
2021
·
IF 3.196
Ting-Hong Huang,Cheng Luo,Dan Zheng,Jing-Yang Tan,Yong Liu,Jing Tang
Based on α- and β-naphthylamine, two new diphosphines, N,N-bis((diphenylphos phino)methyl)naphthalen-1-amine ( 1 ) and N,N-bis((diphenylphosphino)methyl)naphthalene -2-amine ( 2 ), have been prepared and characterized by IR, 1 H NMR, 31 P NMR, TG, UV-Vis, XRD and X-ray crystal structure analysis. Structural analysis shows that the two Ph 2 PCH 2 - groups in 1 and 2 are disposed in an anti configuration, and the change of the position of the (Ph 2 PCH 2 ) 2 N-group on the naphthalene moiety results in different spatial configurations of the two Ph 2 P- groups and different weak interactions, such as C-H … π and π–π interactions. DFT studies show that the composition of the HOMOs for 1 and 2 are almost the same (the naphthyl group with admixed N and P atoms), but the ingredients of the LUMOs for 1 are different from that for 2, coinciding with the change of Mülliken atomic charges . Moreover, the emission spectra show that the emissions of complexes 1 and 2 in acetonitrile solution are not similar to those of solid-state samples.
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高影响因子
JOURNAL OF ORGANIC CHEMISTRY
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2021
·
IF 4.354
Yuxiu Li,Xiangqian Li,Xiaowei Li,Dayong Shi
The straightforward synthesis of α-fluoro-β-arylalkenyl sulfones under transition-metal- and base-free conditions has been described, which displays broad functional group compatibility and high stereoselectivity. In particular, the strategy is also applied to the late-stage modification of complex natural products and drugs.
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PubMed
高影响因子
INDUSTRIAL & ENGINEERING CHEMISTRY RESEARCH
·
2022
·
IF 4.326
Xuewu Liu,Xingfei Li,Yuxiang Bai,Xing Zhou,Long Chen,Chao Qiu,Cheng Lu,Zhengyu Jin,Jie Long,Zhengjun Xie
This study presents a simple method to immobilize β-agarase onto amino-modified magnetic nanoparticles (MNPs) by biotin/streptavidin (BT/SA) recognition. The effect of BT functionalized with different lengths of poly(ethylene glycol) (PEG) on the activity and stability of heterogeneous biocatalysts was also investigated. The results showed that the enzymatic activity was retained above 93% under the immobilization conditions (pH 7.0, 25 °C, 6 h, and β-agarase concentration of 1 mg/mL). The optimal pH and temperature for both free and immobilized β-agarases were 7 and 30 °C, respectively. PEG length played an important role in the catalytic performance of the immobilized enzyme. As the PEG length increased, an increased trend in activity retention and yield of neoagarobiose was observed. Ultimately, the introduction of PEG24 improved the activity retention of immobilized β-agarase up to 135.67%, and retained 84.18 and 37.04% of residual activity after incubation for 6 h at 40 and 45 °C, respectively, while only 30.50 and 0% of residual activities were observed for free β-agarase at these temperatures, indicating the significantly enhanced stability of the β-agarase after immobilization. Additionally, β-agarase-BTPEG24-SA@MNPs showed the highest yield of neoagarobiose at 45 °C, which was 1.89-fold higher than that of the free enzyme. These results indicated that immobilization of β-agarase on BTPEG-SA@MNPs improved both the catalytic efficiency and stability of the enzyme, thereby promoting the continuous production of neoagarobiose.
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高影响因子
Analytical Methods
·
2012
·Vol. 4
·期 11
·3785-3791
·
IF 1.547
Essy K. Fodjo,Sara Riaz,Da-Wei Li,Lu-Lu Qu,Niamien P. Marius,Trokourey Albert,Yi-Tao Long
A Surface Enhanced Raman Scattering (SERS) substrate based on Ag/β-AgVO3 nanobelts deposited on copper foil (Cu@Ag/β-AgVO3) was used for the detection of carbamate pesticides: carbofuran, carbaryl, isoprocarb and propoxur. Cu@Ag/β-AgVO3 has shown an excellent SERS activity for carbamate pesticides compared to silver nanoparticles (AgNPs). Under optimized conditions, detection limits of 2.5 pM, 10 pM, 50 pM and 75 pM were obtained for carbaryl, carbofuran, isoprocarb and propoxur respectively, suggesting that Cu@Ag/β-AgVO3 is a good candidate for use as a SERS substrate for the trace level detection of these pesticides.
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高影响因子
JOURNAL OF INCLUSION PHENOMENA AND MACROCYCLIC CHEMISTRY
·
2019
·Vol. 93
·期 3
·289-299
·
IF 1.429
Li Zhendong,Liu Huijun,Qi Caixia,Yang Aohui,Deng Shanxia
Using ethylenediamine-β-cyclodextrin and α-naphthylacetic acid as raw material, a novel inclusion complex (en-β-CD@NAA) was synthesized. The inclusion complex structures and properties were analyzed by reliable spectroscopic and physicochemical techniques. The stoichiometry of the inclusion complex was 1:1 and the stability constant ( K S ) value was found to be 2511 M −1 at 293 K. Molecular thermodynamic parameters indicated that the spontaneous and exothermic nature of the process. The inclusion form was foreseen by Chem 3D program combined with 1 H-NMR experimental data. The solubilization curves ( K 1 = 2.05) and in-vitro dissolution data demonstrated that the en-β-CD@NAA inclusion complex has satisfactory water solubility.
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高影响因子
ACS Food Science & Technology
·
2022
·
IF 0
Feng Han,Qing-ping Chen,Guangxin Yang,Wenlei Zhai,Essy Kouadio Fodjo,Liang-liang Tian,Hui-juan Yu,Yong-fu Shi,Cong Kong
An effective and efficient method for the determination of 18 β-agonists in aquatic products using liquid chromatography tandem mass spectrometry (LC-MS/MS) was successfully established. The method optimization was conducted based on incurred samples to guarantee its practicability. In this method, the sample was hydrolyzed with β-glucuronidase-arylsulfatase in ammonium acetate solution and treated successively with protein precipitation in 5% trichloroacetic acid in water, lipid removal with n-hexane, and further concentration and cleanup by solid-phase extraction (hydrophilic–lipophilic balanced cartridge, primary–secondary amine cartridge). Isotope-labeled internal standards and matrix-matched calibration curves were used for quantification. The limit of quantification (LOQ) was verified at 0.5–1.0 μg/kg (S/N > 10). This method was evaluated with five different matrices, including eel, roasted eel, pufferfish, shrimp, and crab, at 1×, 2×, and 10× LOQs, with good recoveries (>77%) and high replicate repeatability (relative standard deviations (RSDs) <13%, intraday batch repeatability) and further validated by three other accredited laboratories. A total of 3 samples out of 37 tested aquatic products were found positive for unknown incur sources (ractopamine, 0.38–2.1 μg/kg; salbutamol 3.0 μg/kg; tulobuterol 2.9 μg/kg; clenbuterol 1.3 μg/kg; and clenpenterol, 0.3 μg/kg). The method has demonstrated to be reliable, sensitive, and stable for multiresidue screening of β-agonists for food safety monitoring.
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DOI
ACS Applied Materials & Interfaces
·
2023
·
IF 9.5
Jiahao Shi,Yuhe Zhang,Bin Ma,Haiyang Yong,Delu Che,Chaolan Pan,Wei He,Dezhong Zhou,Ming Li
Poly(β-amino ester)s (PAEs) have been widely developed for gene delivery, and hydrophobic modification can further enhance their gene transfection efficiency. However, systematic manipulation of amphiphilicity of PAEs through copolymerization with hydrophobic monomers is time-consuming and, to some extent, uncontrollable. Here, a modular strategy is developed to manipulate the amphiphilicity of the PAE/DNA polyplexes. A hydrophobic polymer (DD-C12–122) and a hydrophilic polymer (DD-90–122) are synthesized separately and used as a hydrophobic module and a hydrophilic module, respectively. The amphiphilicity of polyplexes could be manipulated by changing the ratio of the hydrophobic module and hydrophilic module. Using the modular strategy, the PAE/DNA polyplexes with the highest gene transfection efficiency and safety profile as well as possible mechanisms are identified. The modular strategy provides a novel way to engineer the hydrophobicity of PAEs to improve their gene transfection and can be easily generalized and potentially extended to other polymeric gene delivery systems.
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PubMed
高影响因子
Crystals
·
2021
·Vol. 11
·期 9
·1049
·
IF 2.589
Jia Xiang,Bo Wang,Le Fu,Chuanpin Chen,Wenjie Liu,Songwen Tan
Lactose is a commonly used excipient with two isomers. Different isomers have different properties, especially in terms of solubility. This work is mainly to explore the influence of different a/β ratio lactose on drug dissolution. This work has developed novel mesoporous pollen-like lactose anhydrous with tailored α/β ratios as ingredient carriers for controlled dissolution rate. The produced lactose carriers are pollen-like with a particle size of ~15 μm and a mean pore width of ~30 nm. β-lactose anhydrous has a unique FTIR-peak at 948 cm−1, whereas α-lactose anhydrous shows a unique FTIR-peak at 855 cm−1. DSC analysis suggests that the pollen-like α/β-lactose crystals are polymorphs with unique peaks of melting points. XRD analysis suggests that (5:5)α/β-lactose polymorph has high crystalline purity. The loading efficiency (30.6–33.4%w/w) of acetamidophenol within the nanoporous lactose particles is dependent on the surface structure and pore volumes—the pore volumes were found to be 0.0209–0.0380 cm3/g. The release rates of acetamidophenol are lower for lactose with high α/β ratios. The lactose solubility and the first-order release constant can be tailored by changing the proportion of β-lactose in the pollen-like lactose carriers.Keywords:pollen-like anhydrous lactose;α/β ratio;solubility;controlled release
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