Stem Cells International
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2015
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IF 2.813
Meihui Chen,Yu Hou,Dingkun Lin
Polydatin, a glucoside of resveratrol, has been reported to possess potent antioxidative effects. In the present study, we aimed to investigate the effects of polydatin in bone marrow-derived mesenchymal stem cells (BMSCs) death caused by hydrogen peroxide (H2O2), imitating the microenvironment surrounding transplanted cells in the injured spinal cord in vitro. In our study, MTT results showed that polydatin effectively prevented the decrease of cell viability caused by H2O2. Hochest 33258, Annexin V-PI, and Western blot assay showed H2O2-induced apoptosis in BMSCs, which was attenuated by polydatin. Further studies indicated that polydatin significantly protects BMSCs against apoptosis due to its antioxidative effects and the regulation of Nrf 2/ARE pathway. Taken together, our results indicate that polydatin could be used in combination with BMSCs for the treatment of spinal cord injury by improving the cell survival and oxidative stress microenvironments.
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JOURNAL OF MEMBRANE SCIENCE
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2017
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IF 6.035
Liang Shen,Jian Zuo,Yan Wang
Forward osmosis (FO) has drawn growing attention in recent years, while the lack of desirable FO membranes has been restricting its further development in industrial applications. In this work, a novel tripodal amine — tris(2-aminoethyl)amine (TAEA), with a dual role of catalyst and reactive amine monomer , is incorporated in the PA selective layer for the first time, to in-situ modify the thin-film composite (TFC) membrane. A series of characterization techniques are employed to investigate the modification mechanism involved, as well as changes in chemical properties and the microstructure of the PA layer in terms of the TAEA content and the amine solution pH. The separation performance and anti-fouling behavior of the TAEA-modified TFC membranes are studied correspondingly. In comparison with the control TFC membrane, modified TFC membranes possess higher water permeability, higher salt rejection, and much lower fouling propensity, and may hold a great potential for FO applications.
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GREEN CHEMISTRY
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2017
·Vol. 19
·期 4
·1102-1108
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IF 9.125
Chunqing Lou,Ning Zhu,Ronghua Fan,Hailong Hong,Limin Han,Jianbin Zhang,Quanling Suo
A convenient and efficient method for the synthesis of 2-mercaptobenzothiazoles from disulfide and CS2 mediated by a metal sulfide in water is described. This synthetic methodology could be used to prepare diverse 2-mercaptobenzothiazole derivatives in good to excellent yields. In this paper, the concept of metal sulfide–disulfide dynamic interchange reaction was put forward. Then the intermediates of the interchange reaction between NaHS and a disulfide were detected by LC-MS, which demonstrated that the S–S bond of the disulfide could be broken by the metal sulfide through the dynamic interchange reaction. In addition, NaHS was eventually transformed into sulfur S8 by the dynamic interchange reaction. Moreover, the underlying mechanism of 2-mercaptobenzothiazole formation is proposed, in which NaHS not only acts as an S–S bond cleaving agent but also as an activator of CS2. As a result, a novel synthetic route for the preparation of sulfur-containing heterocycles from a disulfide is developed.
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MOLECULAR NUTRITION & FOOD RESEARCH
·
2025
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IF 4.2
Fang Wu,Yaqiong Liu,Ming Zhang,Xinlei Yuan,Yutong Jin,Yixuan Li,Ran Wang,Yanling Hao,Bing Fang
1,3-Dioleoyl-2-palmitoyl-glycerol (OPO) is a specific triglyceride in human breast milk, and it has been added to infant formula to mimic human breast milk fat. Existing studies only focused on its effects on fatty acid and calcium absorption, as well as the intestinal microbial composition; however, effects of OPO on the early-life development of intestine were still unclear. Our study explored the effects of OPO on intestinal epithelial structure and barrier construction in neonatal mice and the involvement of intestinal microorganisms. OPO supplementation significantly increased the number of intestinal stem cells, which in turn promoted villus and crypt, and promoted goblet cell and Paneth cell differentiation. OPO also promotes epithelial barrier integrity by increasing the expression of mucin 2, lysozyme 1, and tight junction proteins. Furthermore, the benefits of OPO were associated with the higher abundance of beneficial bacteria ( unclassified_f_Muribaculaceae , Akkermansia , Bifidobacterium , and Blautia ) and elevated butyrate levels. This study demonstrates the efficacy of OPO on intestinal health in neonatal mice beyond defecation, expands the understanding of the biological functions of OPO, and expands its application in intestinal health products targeting special populations, such as the elderly or individuals with intestinal fragility or injury.
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TALANTA
·
2024
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IF 5.6
Sijin Cai,Xingpeng Huang,Manyu Luo,Deshou Xiong,Wei Pang,Meiling Wang,Li Wang,Shuang Li,Peng Luo,Zhixian Gao
Sensitive detection of ammonia in the environment is crucial due to its potential danger to human ecology and health. In gas detection technology, resistive sensors utilizing golden cross finger electrodes combined with gas-sensitive materials are commonly employed. In this study, we demonstrated a room-temperature sensor for ambient ammonia detection. The sensor is composed of two-dimensional layer-stacked metal-organic framework (MOF) Cu 3 (HITP) 2 nanomaterials drop-coated onto gold-forked finger electrodes. Density-functional theory simulation (DFT) and sensor gas-sensitive performance testing were conducted for characterization. The sensor exhibited high sensitivity, selectivity, low detection limit, excellent reproducibility, and stability. This can be attributed to the abundant Cu active sites exposed in the hexagonal ring and layer-stacked framework structure of Cu 3 (HITP) 2 nanomaterials. Ammonia adsorption leads to electron transfer into the Cu 3 (HITP) 2 framework, resulting in decreased sensor resistance. Real-time monitoring of sensor resistance changes enabled quantitative analysis. Results showed a 91.4 % response of the Cu 3 (HITP) 2 sensor to 100 ppm NH 3 , with response and recovery times of 26 s and 20 s, respectively. The sensor's limit of detection (LOD) was approximately 15 ppb. The sensor exhibited a relatively high response to NH 3 at 25 °C, as demonstrated by dynamic gradient test curves. These findings suggest that constituting a room-temperature ammonia sensor by uniformly drop-coating Cu 3 (HITP) 2 onto a gold-forked finger electrode is a feasible strategy.
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TALANTA
·
2024
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IF 5.6
Hongtao Feng,Siyi Min,Shuguang Xuan,Zhuofei Gan,Zhao Sun,Yu Gao,Shuang Yang,Wen-Di Li,Yan Chen
Compact and user-friendly nucleic acid biosensors play a crucial role in advancing infectious disease research, particularly for coronavirus (COVID-19). While nanophotonic metasurface sensors hold promise for high-performance sensing, they face challenges due to their complexity and bulky readout instruments. In this study, we propose a gradient nanoplasmonic imaging (GNI) metasurface that incorporates the concept of an optical potential well, enabling label-free single-step detection of SARS-CoV-2 sequences. The metasurface sensor consists of nanopillars with continuous variations, forming an optical potential well that results in a centimeter-scale dark ring. This dynamic well exhibits high sensitivity to refractive index changes , recorded by a CCD. To further enhance the visualized sensing performance, plasmonic coupling of gold nanoparticles with the gold nanostructure is employed. Our metasurface-based biosensor achieves rapid single-step detection of SARS-CoV-2 sequences, with a low detection limit of 77.2 pM and a detection range of 0.1–100 nM. This biosensor not only demonstrates exceptional reproducibility and outstanding detection performance, but also maintains remarkable specificity in differentiating SARS-CoV-2 from other diseases with similar symptoms. This simple and spectrometer-free refractometric sensing scheme enables the construction of a compact and cost-efficient prototype. Our imaging-based metasurface biosensing strategy demonstrates valuable merits for rapid, sensitive, and quantitative detection, showcasing its potential as a valuable on-site nucleic acid diagnostic tool.
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MICROCHEMICAL JOURNAL
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2024
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IF 4.9
Yansheng Liu,Haonan Hu,Xiaobo Jia,Jin Zhou,Hongli Li,Xiaohong Wang,Shaohui Zhang,Haixin Chang,Fuchong Li,Guofu Wang
This study reported a sandwich SERS structure in detecting SARS-CoV-2 spike protein (SARS-CoV-2 S protein) with high performance. The periodic Ag nanoarrays (AgNAs) functionalized with angiotensin-converting enzyme 2 (ACE2) were utilized as the bottom structure to specifically bound to the SARS-CoV-2 S protein. A complex of SARS-CoV-2 S protein antibody and 4-mercaptobenzoic acid (4-Mba) modified Au nanoparticles (AuNPs@4-Mba@Antibody) were used as nanotags and top SERS enhancing structures. By utilized the “hot spots” in parallel and perpendicular directions, the SERS signals of the 4-Mba as reporters were largely enhanced. By modulated the diameters and the heights of the AgNAs, the SERS performance of the sandwich structures was optimized by using R6G as a stand analyst with limits of detection (LOD) of 1 × 10 −9 M. By applied the optimized sandwich structures in detecting SARS-CoV-2 S protein in phosphate buffered saline (PBS) and pharyngeal swabs solution (PSS), the proposed sandwich structure illustrates a high performance, and the LODs were 0.1 fg mL −1 and 1 fg mL −1 , respectively. This study presented an efficient way to construct a sandwich SERS structure in directly detecting SARS-CoV-2 S protein and this method illustrates a good potential in viral detection.
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Cell Metabolism
·
2024
·
IF 27.7
Kan Huang,Zilun Li,Xi He,Jun Dai,Bingding Huang,Yongxia Shi,Dongxiao Fan,Zefeng Zhang,Yunchong Liu,Na Li,Zhongyu Zhang,Jiangyun Peng,Chenshu Liu,Renli Zeng,Zhipeng Cen,Tengyao Wang,Wenchao Yang,Meifeng Cen,Jingyu Li,Shuai Yuan,Lu Zhang,Dandan Hu,Shuxiang Huang,Pin Chen,Peilong Lai,Liyan Lin,Jielu Wen,Zhengde Zhao,Xiuyi Huang,Lining Yuan,Lifang Zhou,Haoliang Wu,Lihua Huang,Kai Feng,Jian Wang,Baolin Liao,Weiping Cai,Xilong Deng,Yueping Li,Jianping Li,Zhongwei Hu,Li Yang,Jiaojiao Li,Youguang Zhuo,Fuchun Zhang,Lin Lin,Yifeng Luo,Wei Zhang,Qianlin Ni,Xiqiang Hong,Guangqi Chang,Yang Zhang,Dongxian Guan,Weikang Cai,Yutong Lu,Fang Li,Li Yan,Meng Ren,Linghua Li,Sifan Chen
Thrombosis represents the leading cause of death and disability upon major adverse cardiovascular events (MACEs). Numerous pathological conditions such as COVID-19 and metabolic disorders can lead to a heightened thrombotic risk; however, the underlying mechanisms remain poorly understood. Our study illustrates that 2-methylbutyrylcarnitine (2MBC), a branched-chain acylcarnitine, is accumulated in patients with COVID-19 and in patients with MACEs. 2MBC enhances platelet hyperreactivity and thrombus formation in mice. Mechanistically, 2MBC binds to integrin α2β1 in platelets, potentiating cytosolic phospholipase A2 (cPLA2) activation and platelet hyperresponsiveness. Genetic depletion or pharmacological inhibition of integrin α2β1 largely reverses the pro-thrombotic effects of 2MBC. Notably, 2MBC can be generated in a gut-microbiota-dependent manner, whereas the accumulation of plasma 2MBC and its thrombosis-aggravating effect are largely ameliorated following antibiotic-induced microbial depletion. Our study implicates 2MBC as a metabolite that links gut microbiota dysbiosis to elevated thrombotic risk, providing mechanistic insight and a potential therapeutic strategy for thrombosis.
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Food Bioscience
·
2024
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IF 4.8
Zeyu Wu,Jiting Gao,Chongwei Yu,Wei Zhao,Nan Chen,Teresa G. Valencak,Daxi Ren
Diabetes mellitus is a chronic metabolic disease characterized by hyperglycemia, with its prevalence escalating. It exerts a profound impact on human health care and socio-economic development. Probiotics, known as natural, safe, and minimal side effects, have emerged as promising intervention agents to manage chronic diabetes. Our study aimed to explore the therapeutic potential of Lactobacillus rhamnosus ZJUIDS07 for mitigating T2D in mice induced by a high-fat diet (HFD) and streptozotocin (STZ). Administration of L. rhamnosus ZJUIDS07 led to a reduction in diabetic symptoms, including reversal of weight loss, improvement in hyperglycemia and insulin resistance, mitigation of disruptions in glucose and lipid metabolism, alleviation of pancreatic pathological damage, and reduction in oxidative stress and inflammation. Moreover, treatment with L. rhamnosus ZJUIDS07 could restore intestinal microbiota balance in T2D mice by altering the structure and composition of intestinal microbiota, and increased short-chain fatty acids (SCFAs) levels. Specifically, L. rhamnosus ZJUIDS07 significantly decreased the abundance of harmful bacteria like Staphylococcus , Corynebacterium , and Odoribacte , while increasing the abundance of beneficial bacteria (especially SCFAs-producing bacteria) such as Muribaculaceae_norank, Dubosiella , Clostridium sensu stricto 1, Lactobacillus , and Faecalibaculum . Our findings highlight that L. rhamnosus ZJUIDS07 represents a promising probiotic strategy for future treatment of T2D, and that the modulation of gut microbiota and their metabolites through the microbiome-gut-pancreas axis play a pivotal role for the antidiabetic effects of L. rhamnosus ZJUIDS07.
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Microbiology Spectrum
·
2024
·
IF 3.7
Yvhao Xie,Xinxin Li,Qingshi Meng,Jinjun Li,Xin Wang,Liying Zhu,Weiwei Wang,Xiaoqiong Li
Tryptophan (TRP) metabolites have been identified as potent biomarkers for complications of type 2 diabetes mellitus (T2DM). However, it remains unclear whether the therapeutic effect of metformin in T2DM is related to the modulation of TRP metabolic pathway. This study aims to investigate whether metformin affects TRP metabolism in T2DM mice through the gut microbiota. A liquid chromatography-tandem mass spectrometry method was established to determine 16 TRP metabolites in the serum, colon content, urine, and feces of T2DM mice, and the correlations between metabolites and the T2DM mice gut microbiota were performed. The method demonstrated acceptable linearity (R2 > 0.996), with the limit of quantification ranging from 0.29 to 69.444 nmol/L for 16 analytes, and the limit of detection ranging from 0.087 to 20.833 nmol/L. In T2DM mice, metformin treatment effectively restored levels of indole-3-lactic acid (ILA), indole-3-propionic acid (IPA), and the ILA/IPA ratio, along with several aryl hydrocarbon receptor ligands in the serum, with a notable impact in the colon but not in the urine. This restoration was accompanied by a shift in the relative abundance of Dubosiella, Turicibacter, RF39, Clostridia_UCG-014, and Alistipes. Spearman’s correlation analysis revealed positive correlations between Turicibacter and Alistipes with IPA and indole-3-acetic acid. Conversely, these genera displayed negative correlations with ILA and kynurenine. In addition, our study revealed the presence of endogenous indole pathway in germ-free mice, and the impact of metformin on endogenous TRP metabolism in T2DM mice cannot be disregarded. Further research is needed to investigate the regulation of TRP metabolism by metformin.
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JOURNAL OF POWER SOURCES
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2024
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IF 8.1
Zhongxu Chen,Guancheng Xu,Jiahui Jiang,Qihao Wu,Shuai Liu,Yuying Feng,Yixuan Li,Li Zhang,Xian Sun
Poor stability caused by side reactions such as corrosion, Zn dendrite and hydrogen evolution are crucial factors hindering commercial application in queous zinc ion energy storage systems. This article offers a strategy, zinc-rich artificial solid electrolyte interphase (ASEI) layer with polar groups by imidazole-2-carboxaldehyde (2-ICA) electrolyte additive, which helps form stable electrode/electrolyte interface and rapid Zn 2+ transfer interface with low resistance. Firstly, the chelation with Zn 2+ of 2-ICA and its zincophilic capability enable the rapid formation of the ASEI layer and result in more sites for initial nucleation, which then guides the uniform Zn 2+ deposition. Additionally, the introduced ASEI with polar groups accelerates the de-solvation kinetics of Zn 2+ and the Zn 2+ transfer ability in electrode/electrolyte interface, which enable high-rate charge and discharge. Therefore, the symmetric battery based on 2-ICA electrolyte exhibits excellent cycling stability at 20 mA cm −2 (cycling up to 800 h). At the same time, assembled Zn//Cu also has a high average Coulombic efficiency (99.2 %). The zinc-ion hybrid capacitors (ZICs) assembled based on this electrolyte also exhibit outstanding electrochemical properties. This work offers a simple and novel way to realize high performance and great safety zinc-based energy storage devices.
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JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE
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2024
·
IF 3.3
Wenxian Liu,Liting Xu,Haina Cheng,Zhu Chen,Hongbo Zhou,Yuguang Wang
BACKGROUND The potential for the efficient conversion of lignocellulosic biomass has been extensively explored to produce a wide range of bioproducts. Many approaches have been sought for the deep conversion of lignin to generate products that are toxin-free and beneficial for processing into high-value-added components. RESULTS This study reported a fungus isolated from the deep sea with strong synthesis of multiple lignocellulases, conversion of lignin and guaiacol (0.1%) by 71.6% and 86.1% within 9 days at 30 °C respectively, and outstanding environmental adaptability (20–50 °C and pH 3–8). Metabolic pathway profiling showed that this fungus utilized lignin to rapidly activate multiple ring-opening reactions including the 2,3- and 3,4-cleavage pathways, with the 2,3-cleavage pathway predominating after 5 days. Conversion of metabolic intermediates confirmed the superb potential of this strain for lignin treatment. Meanwhile, its shikimic acid pathway was metabolically active under lignin. CONCLUSION This further expands the potential to produce valuable bioproducts during lignin treatment, especially under ambient conditions, which can significantly enhance high-value precursor compound production. © 2024 Society of Chemical Industry.
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