JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
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2017
·
IF 2.603
Lingli He,Chuan Li,Xuyuan Liu,Qingqing Yang,Haizhi Zhang,Weiren Xu,Luyong Zhang,Changxiao Liu
Benzbromarone is a uricosuric drug metabolized predominantly by cytochrome P450 2C9 from in vitro findings. Human CYP2C9 exhibits extensive genetic polymorphism and numbers of clinic studies have demonstrated that CYP2C9 genetic polymorphism has a significant influence on the pharmacokinetics of benzbromarone. But in vitro study on the interaction between CYP2C9 allelic isoforms and benzbromarone was rare. Here, an LC–MS/MS method was established and validated to determine the concentration of benzbromarone in different CYP2C9 enzyme incubation systems for the drug-enzyme interaction study. By selecting appropriate internal standard and optimizing separation system, including mobile phase , sample solvent and gradient elution condition, this LC–MS/MS method was developed with fine linearity (r 2 ≥ 0.996), good reproducibility (RSD ≤ 6.6%), high stability (92.37–114.67%), efficient recovery (91.23–109.82%) and acceptable matrix effect (110.54–115.31%). Based on this method, the interaction between 3 CYP2C9 allelic isoforms and benzbromarone was researched by kinetics parameters (K m , V max , Cl int ). As a result, CYP2C9*1 displayed the highest metabolic activity towards benzbromarone, CYP2C9*2 showed a little lower catalytic activity than CYP2C9*1 (relative clearance/*1 = 85.86%), CYP2C9*3 showed the lowest catalytic activity (relative clearance/*1 = 21.57%). The result illustrated that various CYP2C9 allelic isoforms showed different enzymatic activities towards benzbromarone, which could offer effective consultation for personalized administration in clinic.
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高影响因子
JOURNAL OF MOLECULAR LIQUIDS
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2017
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IF 3.648
Likang Fu,Libo Zhang,Shixing Wang,Jinhui Peng,Gengwei Zhang
A novel adsorbent was synthesized via modifying silica nanoparticles with 3-Amino-5-mercapto-1,2,4-triazole and was used to selectively adsorb silver ions from aqueous solutions. The adsorbent was confirmed by Fourier transform infrared spectroscopy , X-ray photoelectron spectroscopy, transmission electron microscope and Brunauer-Emmett-Teller (BET) surface area analysis. Batch adsorption experiments were carried out to evaluate the adsorption conditions, reusability and selectivity . The results showed that the optimum pH is 5 and the maximum adsorbability was 124.52 mg/g. The adsorption rate was very high and equilibrium was achieved within 20 min. The total adsorption process preferably followed the pseudo-second order kinetics and the Langmuir isotherm model. The sorbent was stable and efficient. After used five times, the adsorbability was 98.72% of the initial adsorbability. Moreover, the adsorbent adsorb selectively Ag + from coexisting ions in aqueous solution.
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ChemCatChem
·
2016
·Vol. 9
·期 3
·458-464
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IF 4.724
Ting Yang,Lan-Lan Lou,Wenjun Yu,Yajun Feng,Hao Li,Kai Yu,Shuangxi Liu
Graphical Porous but extraordinarily durable : 3 D ordered macroporous alumina–carbon composite supported Pt nanocatalysts are applied in the asymmetric hydrogenation of ethyl pyruvate. They exhibit high catalytic activity and extraordinary durability. The three-dimensionally ordered macroporous alumina-carbon composite materials were firstly prepared through a surfactant-assisted colloidal crystal template method and applied to support Pt nanoparticles. The results of SEM, XRD, TEM, and N 2 sorption analyses revealed that these composite materials possessed highly ordered macroporous structures with meso- and micropores within the skeletons of macropores and that the Pt nanoparticles were well dispersed on these materials with a very narrow size distribution. These prepared Pt nanocatalysts were evaluated in the asymmetric hydrogenation of ethyl pyruvate after chiral modification with cinchonidine. Excellent catalytic activity and enantioselectivity were achieved. More importantly, these catalysts exhibited extraordinary stability and reusability during the reaction and could be reused at least 26 times without significant loss in catalytic activity and enantioselectivity.
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RSC Advances
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2016
·Vol. 6
·期 107
·105723-105728
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IF 3.289
Cheng Chen,Yan Li,Lijian Liu
Oligomers as fluorescent macromolecules are of significant importance and are fairly attractive for high quantum yield two-way conversion luminescence. We report a high conversion rate (ΦF = 0.5184) for a fluorescent oligomer, 1,4-butanediol-bis(5-carbonyl-3-carbethoxy-2-pyrazoline). It was prepared by the novel cyclopolycondensation of 1,4-butanediol-bis(5-carbonyl-3-carbethoxy-2-pyrazoline), a chromophore with good fluorescence (ΦF = 0.0573). After polymerization, a green fluorescent protein (GFP)-mimetic structure was formed, in which a methylene chain (–CH2–CH2–CH2–CH2–) as a flexible group is beneficial due to its folding, and an azo group sits in the rigid pyrazoline ring because of a resonance effect. These factors collectively contribute to the excellent efficiency of the fluorescence performance of the oligomer. Additionally, the oligomer exhibited a weak red shift and a significant fluorescence enhancement compared with the monomer, and we achieved good quality live-cell imaging. The oligomer also allowed for the enhancement of fluorescence intensity through the two-wavelength excitation of a cell rather than increasing the dosage of the dye or the power of the laser.
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MICROCHEMICAL JOURNAL
·
2024
·
IF 4.9
Lixia Luo,Jinglin Shui,Yingying Gu,Dandan Wang,Jiangyang Qin,Yanan Wang,Yong Mei
In order to detect the cervical cancer biomarker squamous cell carcinoma antigen (SCCA) in human serum, an electrochemical immunosensor was constructed. Zirconium 1,4-dicarboxybenzene MOF (UiO-66), with a high specific surface area, was coupled with gold nanoparticles (AuNPs), creating the UiO-66@AuNPs nanomaterial. This coupling increased the sensitivity of the electrochemical immunosensor by providing many biological binding sites for antibodies to bind to Au-S bonds. Then, mesoporous carbon (CMK-3) was added to the UiO-66@AuNPs nanomaterial to obtain a CMK-3@UiO-66@AuNPs nanocomposite. This further increased the electron transfer proficiency of the material and enhanced the electrochemical signal. Finally, the sensitive detection of squamous cell carcinoma antigen (SCCA) was made possible by unique immunological responses between antigens and antibodies. Transmission electron microscopy (TEM) analysis of CMK-3@UiO-66@AuNPs revealed embedded surfaces and voids; cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS) confirmed the successful assembly of the electrochemical immunosensor. The developed electrochemical immunosensor demonstrated good linearity (R 1 2 = 0.999) from 0.01 pg/mL to 10,000 pg/mL, which is the concentration range of SCCA in human serum. The limit of detection was 0.0034 pg/mL (at an S/N ratio of 3). The immunosensor exhibited excellent precision, stability and selectivity, with a high rate of recovery (98.26–101.8 %). The experimental validation studies demonstrated that the electrochemical immunosensor was effective for the detection of SCCA in human serum, offering a novel method for the early diagnosis and dynamic surveillance of cervical cancer.
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INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
·
2024
·Vol. 25
·期 3
·1872
·
IF 4.9
Jian Feng,Li Wang,Bo Xiao,Xia Ran,Caiying Wang,Jinming Zhu,Zuoji Liu,Chaozhong Li,Xinai Cui,Rong Li,Guangwei Feng,Zeqin Dai
Photocatalytic technology has been recently conducted to remove microbial contamination due to its unique features of nontoxic by-products, low cost, negligible microbial resistance and broad-spectrum elimination capacity. Herein, a novel two dimensional (2D) g-C3N4/Bi(OH)3(CNB) heterojunction was fabricated byincorporating Bi(OH)3(BOH) nanoparticles with g-C3N4(CN) nanosheets. This CNB heterojunction exhibited high photocatalytic antibacterial efficiency (99.3%) againstEscherichia coli(E. coli) under visible light irradiation, which was 4.3 and 3.4 times that of BOH (23.0%) and CN (28.0%), respectively. The increase in specific surface area, ultra-thin layered structure, construction of a heterojunction and enhancement of visible light absorption were conducive to facilitating the separation and transfer of photoinduced charge carriers. Live/dead cell staining, sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) assays and scanning electron microscopy (SEM) have been implemented to investigate the damage to the cell membrane and the leakage of the intracellular protein in the photocatalytic antibacterial process. The e−, h+and O2•−were the active species involved in this process. This study proposed an appropriate photocatalyst for efficient treatment of bacterial contamination.
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FOOD HYDROCOLLOIDS
·
2024
·
IF 11
Kaixin Li,Xin Yuan,Jiajia Zhao,Jinbo Ren,Lingjun Ma,Xiaojun Liao,Xiaosong Hu,Fang Chen,Junfu Ji
The unique structural of anthocyanins makes them vulnerable to degradation within food processing environments. Proteins commonly coexist with polyphenols in natural food matrices on a non-covalent and covalent basis, serving to stabilize protection for polyphenols. Here, three natural pea-extracted proteins, including albumin, vicilin, and legumin were bound to cyanidin-3-O-glucoside (C3G), and binding modes, conjugate structures and functions were characterized. Isothermal titration calorimetry showed a strongly exothermic interaction between C3G and proteins. The results of sodium dodecyl sulfate-polyacrylamide gel electrophoresis and fourier transform infrared spectroscopy revealed that the C3G-quinone (C3GQ) formed by C3G oxidation established covalent cross-links with C–S and C–N in the amino acid residues of proteins, and could form more large molecular weight aggregates at an addition amount of 1 mM. The free sulfhydryl and amino contents of the three protein conjugates decreased with the addition of C3GQ. Due to the presence of 4–5 times more sulfhydryl groups in albumin as compared to globulin, albumin showed a 20% higher binding capacity for C3GQ than globulin. This consequently led to the highest antioxidant activity in the C3GQ-albumin conjugate. In contrast to native pea proteins, the introduction of covalent modifications induces changes in the secondary structure of the pea proteins. Specifically, the structure of albumin tends to exhibit increased orderliness, while the molecular flexibility of globular proteins was augmented. Thus, the conjugates have enhanced thermal stability. Our results affirmed that the stability of C3G can be more effectively improved through designing it to covalently interact with proteins relative to non-covalent interactions. This study aimed at offering novel theoretical underpinnings for the functional utilization of C3G and pea proteins in the food industry.
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2025
·
IF 8.5
Kai Yang,Zhenjie Tang,Chong Zhang,Ruide Zhang,Sheng Hu,Changjiang Lv,Jun Huang,Jiaqi Mei,Weirui Zhao,Lehe Mei
Salvianic acid A (SAA) is a catechol compound known for its diverse physiochemical functions and has significant applications in the food and pharmaceutical industries. 4-Hydroxyphenylacetate-3-hydroxylase (4HPA3H) is a critical enzyme for SAA biosynthesis, and improving its activity towards p -hydroxyphenyllactate acid (4HPLA) is essential for highly efficient SAA production in stable biosynthetic pathways. To address this, the distal site and loops of the substrate pocket were modified to improve 4HPA3H catalytic activity towards 4HPLA using computer-aided molecular modification methods. As a result, we identified and mutated two critical sites in Ec HpaB, a 4HPA3H monooxygenase from Escherichia coli : T398, a substrate loop site, and M205, a distal site. The mutants M205F, T398S, and M205F/T398S enabled 2.51-, 2.07-, and 2.20-fold increases in catalytic efficiency ( k cat / K m towards 4HPLA), respectively. Molecular dynamics simulations showed that the overall decreased flexibility of the substrate pocket loop in Ec HpaB might help improve the enzyme's catalytic activity for 4HPLA. Owing to the T398 site in the substrate pocket coming from another monomer, molecular modification of Ec HpaB with multi-monomer interactions was also worthy of attention. These findings not only enhanced the biosynthetic efficiency of SAA but also provided insights into enzyme distal site modification and the engineering of multi-monomer enzymes.
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Advanced Science
·
2024
·
IF 14.3
Qiu-Ling Li,Xin Chang,Yu-Mo Han,Zi-Chao Guo,Yi-Na Liu,Bin Guo,Chang Liu,Bin-Rong Yang,Zhong-Kai Fan,Hu-Lin Jiang,Xin Chang
The limited and backward diagnostic approaches elicit high mortality associated with pulmonary fibrosis (PF) because they fail to identify injury phase of PF. Developing a precisely theranostic nanoplatform presents a promising shortcut to reverse PF. Herein, a specific molecular nanotheranostic (Casp-GNMT), which is triggered by endogenous cysteinyl aspartate specific proteinase-3 (caspase-3), boosts antifibrotic efficacy through bioimaging synergistic with chemotherapy at molecular level, facilitating by ionizable lipid and reactive oxygen species sensitive lipid for precise and manageable therapy. The activation of molecular imaging probe (pCY-pairs) by consumption of endogenous caspase-3 initiates fluorescence resonance energy transfer-guided theranostic pattern, aiming to restore mitochondrial dysfunction-induced oxidative stress and inflammatory responses in alveolar epithelial cells II (AECs II). This process sequentially resists the expression of interleukin-1β and vascular endothelial growth factor receptor through combined with nintedanib, further suppressing abnormal injury of AECs II and persistent migration and proliferation of inflammatory cells. Especially, the homeostasis of injured AECs II diminishes excessive accumulation of transforming growth factor-β to restrain myofibroblasts proliferation and collagen deposition, thereby amplifying the possibility of reversing PF. This theranostic nanoplatform is proposed to provide a prompt and exact approach to enhance diagnostic authenticity and treating efficiency through harnessing endogenous indicator for PF reversal.
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Advanced Healthcare Materials
·
2024
·
IF 10
Jie Tang,Yu Li,Xuefeng Hu,Wei Hua,Haoning Xu,Li Li,Fujian Xu
The systemic use of tranexamic acid (TA) as an oral drug could bring adverse reactions, while intradermal injection leads to pain and a risk of infection. Moreover, it is difficult for highly hydrophilic TA to penetrate the skin barrier that contains lots of hydrophobic lipid compounds, which poses enormous restrictions on its topical application. Current transdermal TA delivery strategies, are suffering from low drug load rates, plus its synthesis complexity, time-consumption etc., adding to the difficulty of TA topical application in clinical therapeutics. To increase the penetration of TA, a novel approach using TA-loaded ZIF-8 (TA@ZIF-8) was developed. The encapsulation efficiency of TA@ZIF-8 reached nearly 25% through physical adsorption and chemical bonding of TA indicated by theoretical simulation and the improved TA penetration was elevated through activating the aquaporin-3 (AQP-3) protein. Additionally, in vivo and in vitro experiments have demonstrated the preponderance of TA@ZIF-8 for penetration ability and the advantages in intracellular uptake, minor cytotoxicity, and inhibition of melanogenesis and inflammatory factors. Moreover, clinical trials have demonstrated the safety and efficacy of TA@ZIF-8 in the treatment of melasma and rosacea. This work presents a potential topical application of TA, free from the safety concerns associated with systemic drug administration. This article is protected by copyright. All rights reserved
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Molecular Metabolism
·
2024
·
IF 7
Junyong Wang,Yaping Guo,Yunduan He,Yifan Qin,Xiuling Li,Ling Yang,Kangdong Liu,Li Xiao
Objective Nonalcoholic fatty liver disease (NAFLD) is an emerging public health threat as the most common chronic liver disease worldwide. However, there remains no effective medication to improve NAFLD. G protein-coupled receptors (GPCRs) are the most frequently investigated drug targets family. The Regulator of G protein signaling proteins 14 (RGS14), as an essential negative modulator of GPCR signaling, play important regulatory roles in liver damage and inflammatory responses. However, the role of RGS14 in NAFLD remains largely unclear. Methods and Results In this study, we found that RGS14 was decreased in hepatocytes in NAFLD individuals in a public database. We employed genetic engineering technique to explore the function of RGS14 in NAFLD. We demonstrated that RGS14 overexpression ameliorated lipid accumulation, inflammatory response and liver fibrosis in hepatocytes in vivo and in vitro . Whereas, hepatocyte specific Rgs14 -knockout ( Rgs14 -HKO) exacerbated high fat high cholesterol diet (HFHC) induced NASH. Further molecular experiments demonstrated that RGS14 depended on GDI activity to attenuate HFHC-feeding NASH. More importantly, RGS14 interacted with Guanine nucleotide-binding protein (Gi) alpha 1 and 3 (Giα1/3, gene named GNAI1/3 ), promoting the generation of cAMP and then activating the subsequent AMPK pathways. GNAI1/3 knockdown abolished the protective role of RGS14, indicating that RGS14 binding to Giα1/3 was required for prevention against hepatic steatosis. Conclusions RGS14 plays a protective role in the progression of NAFLD. RGS14-Giα1/3 interaction accelerated the production of cAMP and then activated cAMP-AMPK signaling. Targeting RGS14 or modulating the RGS14-Giα1/3 interaction may be a potential strategy for the treatment of NAFLD in the future.
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PETROLEUM SCIENCE AND TECHNOLOGY
·
2025
·
IF 1.4
Airong Li,Pan Liu,Yulong Zhao,Yikai Zou
The exploration of shale gas has increasingly emerged in recent years. In this work, the ionic liquid (IL) of 1-butyl-3-methylimidazole ferric tetrachloride was firstly used as a chemical additive to improve the dissolution of shale and enhance shale gas desorption. The analyses of total organic carbon (TOC), Fourier transform infrared spectroscopy, X-ray diffractometer, X-ray photoelectron spectroscopy, and Brunauer–Emmett–Teller measurement were performed. The results revealed that the TOC content was reduced by 66.2% using a concentration of 0.1 mol/L 1-butyl-3-methylimidazole ferric tetrachloride with a solid-liquid ratio of 1:20 at 60 °C and a duration of 48 hr and the dissolution rate of shale was 18.95 wt%. Inorganic minerals within shale were dissolved, and irregular pores were observed, which improve the permeability of shale and the flow of shale gas. Meanwhile, the characterization of residues in the solution presented that the structures of long carbon chain, carbon-sulfur and amines, amides, or heterocyclic within kerogen may be disrupted, which is beneficial for the shale gas desorption. The isothermal adsorption results proved that the adsorption capacity of methane on the kerogen treated was decreased by 53.16%, which inferred that the desorption ability of shale gas can be improved.
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危险品化学品经营许可证（带存储）