PHOTOCHEMISTRY AND PHOTOBIOLOGY
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2022
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IF 3.521
Yu Zhang,Dekun Cheng,Zhangtie Wang,Yiwen Guo,Ming Chang,Ruijie Liu
Excessive ultraviolet (UV) exposure is the primary environmental factor that contributes to skin aging. Orbitides have been considered as important functional components in linseed, and they are complex and multiple. In this study, linseed orbitides (LOs) were divided into oxidized linseed orbitides (OLOs) and reduced linseed orbitides (RLOs) and used to investigate protection against ultraviolet B-induced photoaging in zebrafish. First, the results of the zebrafish embryo acute toxicity test showed that the OLOs had obvious embryo toxicity compared with RLOs. And RLOs had better effect in ultraviolet B-treated zebrafish, which may significantly reduce the reactive oxygen species levels and inhibit the degradation of collagen. Besides, we also found that RLOs could effectively inhibit the oxidation of proteins and lipids and regulate the activity of antioxidant enzymes. Furthermore, neutrophil recruitment to the dorsal and caudal fin regions was observed in UVB-treated zebrafish, and RLOs effectively alleviated this migration. In conclusion, RLOs have strong photoprotective effects and potential to be used as antiphotoaging ingredients.
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DYES AND PIGMENTS
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2022
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IF 5.122
Meijuan Zhang,Jian Cao,Cuiping Huang,Mingming Liu,Yanan Li,Chuanxiao Wang,Yajing Tu
Human serum albumin (HSA) accounts for 60% of total plasma protein and is closely related to life-threatening diseases. In this strategy, three fluorescent sensors of Nap-OH, Nap-C and Nap-NO 2 based on twisted intramolecular charge transfer (TICT) are designed and synthesized for detecting of HSA, and the effect of electron donating-withdrawing groups of sensors on the detection of HSA is studied. After added HSA, the fluorescent emission of Nap-NO 2 is turned on at 630 nm, and the color changed from colorless to pink. Nap-NO 2 is characterized by a 14.06-fold increase in fluorescence emission and has high sensitivity, good selectivity, fast response, and good stability. Nap-NO 2 could effectively distinguish HSA from other proteins, including bovine serum albumin (BSA). Drug competition experiments are performed that Nap-NO 2 can bind to the IB domain of HSA, avoiding the interference of commonly used drugs. Nap-OH, Nap-C and Nap-NO 2 can also be used for viscosity detection.
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Frontiers in Oncology
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2022
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IF 5.738
Kong Ji chuan,Li Yang,Ma Wang,Du Yu rong,Liu Lei,Qu Tian tian,Liu Shuo shuo,Wang Meng li,Dou Wei
As an effective strategy for oncotherapy, developing efficacious drug delivery systems for cancer combination therapy remains a major challenge. To improve nanodrug biocompatibility and composite function facilitating their clinical conversion application, a novel nanocarrier was presented by a facile method through conjugating humic acid with gadolinium ions to synthesize HA-Gd with good biocompatibility and dispersity. HA-Gd exhibited high photothermal conversion efficiency up to 38%, excellent photothermal stability, and high doxorubicin (DOX) loading capacity (93%) with pH-responsive release properties. HA-Gd loading DOX showed a combined chemo-photothermal inhibitory effect on tumor cells. Compared with lipid-DOX, HA-Gd-DOX had a more significant inhibitory effect on tumor growth and fewer side effects. T1-weighted MRI contrast toward tumor tissue provided HA-Gd with an MRI-based cancer diagnosis. This study revealed the great potential of humic acids as a novel vector for developing more drug carriers with desirable functions for clinical anticancer therapy.
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ACS Applied Materials & Interfaces
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2023
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IF 9.5
Dan Zhao,Xiaoyu Huang,Yanan Tian,Jianhua Zou,Fu Wang,Xiaoyuan Chen
Ferroptosis plays an important role in tumor inhibition and is a new type of programmed cell death. Recent studies have shown that glutathione (GSH) depletion is an effective method to enhance the therapeutic efficacy of ferroptosis; however, a systematic investigation of the phenomenon is limited. Herein, we provide a facile fluorescence imaging-incorporated transcriptome strategy to visualize the process and explore the mechanism of GSH depletion-enhanced ferroptosis. The proposed multifunctional nanoplatform is achieved using simple transferrin receptor aptamer-functionalized fluorescent gold nanoclusters (termed TfRA-AuNCs), which exhibit efficient hydroxyl radical generation and GSH-depleting capabilities. Live cell fluorescence imaging results revealed that TfRA-AuNCs were endocytosed into 4T1 cells and were mostly distributed in lysosomes. In vitro results indicated that TfRA-AuNCs enhanced the ferroptosis effect in 4T1 cells. Importantly, transcriptome analysis indicated that 4T1 cells treated with TfRA-AuNCs regulated the expression change of ferroptosis-related genes, and the Kyoto Encyclopedia of Genes and Genomes pathway identified the GSH metabolism pathway involved in ferroptosis, thus revealing the exact molecular mechanism of ferroptosis induced by TfRA-AuNCs at the RNA level. Furthermore, in vivo results confirmed the tumor inhibition effect, tumor-targeted fluorescence imaging, and long-term biocompatibility after TfRA-AuNC treatment. This study introduces a new possibility for the mechanistic study of nanoagent-induced ferroptosis in tumor treatment.
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JOURNAL OF NANOBIOTECHNOLOGY
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2023
·Vol. 21
·期 1
·1-18
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IF 10.2
Cao Yameng,Cheng Kai,Yang Mei,Deng Zhichao,Ma Yana,Yan Xiangji,Zhang Yuanyuan,Jia Zhenzhen,Wang Jun,Tu Kangsheng,Liang Jie,Zhang Mingzhen
Background Inflammatory bowel disease (IBD) is a chronic nonspecific disease with unknown etiology. Currently, the anti-inflammatory therapeutic approaches have achieved a certain extent of effects in terms of inflammation alleviation. Still, the final pathological outcome of intestinal fibrosis has not been effectively improved yet.Results In this study, dextran-coated cerium oxide (D-CeO2) nanozyme with superoxide dismutase (SOD) and catalase (CAT) activities was synthesized by chemical precipitation. Our results showed that D-CeO2 could efficiently scavenge reactive oxide species (ROS) as well as downregulate the pro-inflammatory cytokines (IL-1β, IL-6, TNF-α, and iNOS) to protect cells from H2O2-induced oxidative damage. Moreover, D-CeO2 could suppress the expression of fibrosis-related gene levels, such as α-SMA, and Collagen 1/3, demonstrating the anti-fibrotic effect. In both TBNS- and DSS-induced colitis models, oral administration of D-CeO2 in chitosan/alginate hydrogel alleviated intestinal inflammation, reduced colonic damage by scavenging ROS, and decreased inflammatory factor levels. Notably, our findings also suggested that D-CeO2 reduced fibrosis-related cytokine levels, predicting a contribution to alleviating colonic fibrosis. Meanwhile, D-CeO2 could also be employed as a CT contrast agent for noninvasive gastrointestinal tract (GIT) imaging.Conclusion We introduced cerium oxide nanozyme as a novel therapeutic approach with computed tomography (CT)-guided anti-inflammatory and anti-fibrotic therapy for the management of IBD. Collectively, without appreciable systemic toxicity, D-CeO2 held the promise of integrated applications for diagnosis and therapy, pioneering the exploration of nanozymes with ROS scavenging capacity in the anti-fibrotic treatment of IBD.
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ACS Sustainable Chemistry & Engineering
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2022
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IF 9.224
Jiaojiao Zhou,Die Zhang,Xuqin Lv,Xiaoqing Liu,Wei Xu,Lei Chen,Jie Cai,Zia-ud Din,Shuiyuan Cheng
Selenium is an essential micronutrient and is well known for its role in regulating metabolism. However, it is also highly toxic in some of its forms and above certain concentration levels. Amorphous selenium nanoparticles (SeNPs) are extremely unstable and convert into non-bioactive or gray elemental selenium with crystal-type structures due to aggregation, which limits its antioxidant and anti-tumor activities. To overcome these limitations, monodisperse SeNPs were obtained by stabilizing SeNPs with glucan and rosmarinic acid (RA) via Se–O bonds and polysaccharide–polyphenol interactions. The prepared glucan-RA-SeNPs were found much more stable than both SeNPs and glucan-stabilized SeNPs. Meanwhile, glucan-RA-SeNPs displayed enhanced bioactivities including an improved free radical scavenging ability and an increased cytotoxicity toward cancer cells. The underlying mechanism might be related to caspase activation by glucan-RA-SeNPs, thus overproducing intracellular reactive oxygen species and eventually resulting in cancer cell apoptosis. Our work proposes a feasible strategy for improving the structure–bioactivity of SeNPs through polysaccharide–polyphenol interaction.
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DIGESTIVE DISEASES AND SCIENCES
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2022
·Vol. 67
·期 10
·4780-4796
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IF 3.487
Duan Houyu,Ding Xiang,Luo Hesheng
Background and Aims KISS-1 is an established tumor suppressor that inhibits metastases in various malignancies. However, little is known regarding its role in esophageal squamous cell carcinoma (ESCC). The aim of the present study was to identify the possible mechanisms of KISS-1 in ESCC metastasis. Methods The expression levels of KISS-1 mRNA and protein in ESCC samples and cell lines were analyzed by qRT-PCR, IHC, and western blotting. Bisulfite sequencing PCR (BSP) and methylation-specific PCR (MSP) were used to analyze the methylation pattern of KISS-1 promoter in ESCC cells with or without 5-Aza-dC treatment. The role of KISS-1 in the progression and metastasis of ESCC was analyzed through in vitro functional assays. Results KISS-1 mRNA and protein were markedly downregulated in ESCC tissues and cell lines compared to the respective controls. Hypermethylation of KISS-1 promoter correlated to its lower expression levels in ESCC, and KISS-1 demethylation inhibited tumor progression. Ectopic KISS-1 overexpression inhibited tumor cell metastasis in vitro. In addition, KISS-1 overexpression downregulated the matrix metalloproteinase 2 and 9 (MMP2 and 9) and inhibited epithelial–mesenchymal transition (EMT). Finally, KISS-1 downregulated phosphorylated extracellular regulated protein kinase 1/2 (ERK1/2) and phosphorylated p38 mitogen-activated protein kinase (MAPK) without affecting their total expression levels in the ESCC cells. MAPK/ERK and p38 MAPK agonists reversed the suppressive effects of KISS-1. Conclusions The hypermethylation of KISS-1 promoter partly contributed to its downregulation in ESCC. KISS-1 inhibits the metastasis of ESCC cells by targeting the MMP2/9/ERK/p38 MAPK axis.
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APPLIED SURFACE SCIENCE
·
2022
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IF 7.392
Zhiwen Huang,María Luisa Cerón,Kehuai Feng,Dan Wang,María Belén Camarada,Xiaoning Liao
Exploration of an efficient ochratoxin A (OTA) screening technique is highly important because of its highly toxic effect on human health. Electrochemical sensor, characterized with high accuracy and precise, is an emerging detection technique for toxic agents in food samples. However, the inherent fouling effect and low electrochemical activity from OTA molecular hinder its practical application in OTA detection. Herein, we prepared a novel 0D/1D nanohybrid by anchoring black phosphorus quantum dots (BPQDs) over carboxylated multiwalled carbon nanotubes (CNTs-COOH) via a facile sonication assisted assembly approach. Characterization techniques together with theoretical approximations reveal that BPQDs can anchor onto the CNTs-COOH via strong covalent reaction, endowing the nanohybrid with high conductivity, high stability and high anti-fouling property. The electrochemical sensor constructed with the 0D/1D nanohybrid shows high electrochemical activity and a good antifouling effect in OTA detection. In optimized conditions, the sensor provide a quantitative determination of OTA in a wide concentration range from 0.4 to 10 μM with a detection limit of 0.03 μM. Moreover, the sensor shows high reproductivity and satisfactory anti-interference ability. Satisfactory recoveries that can comparable to those of high-performance liquid chromatography indicate that the sensor is promising for the trace-level analysis of OTA in agricultural crops.
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ELECTROCHIMICA ACTA
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2022
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IF 7.336
Sankar Sekar,Jiang Huijun,Zhou Liuzhu,Chen Jin,Sejoon Lee,Deuk Young Kim,Ramalingam Manikandan
Foodborne antioxidants including ascorbic acid (AA), caffeic acid (CA) and gallic acid (GA) are beneficial nutritional sources and thereby the analysis of the critical antioxidant is demanding for public health. Here, a sonochemical method was used to obtain hybrid copper-phthalocyanine conjugated graphitic carbon nitrides nanosheets (CuPTc/g-C 3 N 4 Ns) in which carboxylic acids and hydroxyl groups were added on the basal plane of g-C 3 N 4 . As prepared materials were characterized by various physicochemical and electroanalytical techniques, the CuPTc/g-C 3 N 4 Ns nanocomposite showed an excellent electrocatalytic activity towards antioxidants quantifications by using Indium Tin Oxide (ITO) coated glass plate. Under optimized experimental conditions, sensitive electrochemical detection of antioxidants was achieved on the CuPTc/g-C 3 N 4 Ns nanocomposite-modified ITO which resulted in wide linear concentration range of 0.05-500 µM (AA), 0.05-501 nM (CA) and 10-445 nM (GA) and the limit of detection (LOD) of 0.006 µM, 0.008 nM and 0.5 nM respectively. Furthermore, the fabricated sensor was successfully used for the antioxidants determinations in different commercial fruit juice, alcoholic beverages and tea samples with good recovery results demonstrating a hybrid phthalocyanine conjugated 2D carbon nanocomposite as an efficient electrocatalyst of desirable practical importance.
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DYES AND PIGMENTS
·
2022
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IF 5.122
Xunan Jing,Lingjie Meng,Tingting Yang,Ning Zhang,Shu Fan,Yanni Chen,Hongbo Yang,Daquan Wang,Wenchen Ji,Junjun She
Photodynamic therapy (PDT) is more precise than traditional tumor therapy. Nevertheless, PDT efficiency often is hindered by the antioxidant defense system which deplete the required reactive oxide species (ROS). Here we developed a simple but universal method by assembling anticancer drug (resveratrol, RV), photosensitizer (methylene blue, MB) and glutathione (GSH)-sensitive molecules (bis-(4-hydroxyphenyl)-disulfide, HPS) to covalent linking with hexachlorocyclotriphosphazene (HCCP) onto the Fe 3 O 4 to construct a versatile drug delivery nanosystem (FHRHM). Notably, as a multifunctional nanodrug it shows good physiological stability, biocompatibility, and pH/GSH-responsive property to realize the RV and MB controlled release in tumor microenvironment (TME), maximum magnetic resonance (MR)/fluorescence (FL) imaging guided for synergistic chemo-photodynamic therapy (CT-PDT). Especially, the cross-linked FHRHM could be decomposed via reacting with high level of GSH to accelerate release MB for exogenous ROS generation, as well as disrupted disulfide bond (-S-S-) of HPS through depleting GSH to further reduce the endogenous ROS scavenging in TME, thus double amplifying the PDT and simultaneously enhancing CT to induce Hela cells death. This work highlights a novel strategy to construct TME-dissociable nanodrug for augmented CT-PDT via regulation of redox homeostasis, may provide a promising translational approach for tumor theranostics.
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DNA AND CELL BIOLOGY
·
2022
·Vol. 41
·期 4
·424-436
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IF 3.55
Feng Chi,Fang Qiu,Xiaoming Jin,Long Chen,Guijin He,Sijia Han
Breast cancer (BC) is one of the most frequently occurring malignant tumors in female adults. The long intergenic nonprotein coding RNA 00982 (LINC00982) has been regarded as a cancer suppressor in several human cancers. However, the function and the underlying mechanisms of LINC00982 have not been studied in BC. The present study found that LINC00982 was significantly downregulated in BC tumor tissues, and the low LINC00982 level predicts a poor prognosis of BC. Through the overexpression and suppression of LINC00982 in two BC cell lines, we found that LINC00982 could inhibit cell proliferation, migration, and invasion by suppressing the activity of the signal transducer and activator of transcription 3 (STAT3)/nuclear factor kappa B (NF-κB) signal pathway. Furthermore, luciferase reporter assay has been used to verify that LINC00982 functions as a molecular sponge for miR-765, which could target DPF3. The relative expression of miR-765 decreased with LINC00982 overexpressing, and DPF3 increased at the same time.In addition, the suppression of cell malignant phenotype caused by overexpression of LINC00982 can be reversed by inhibition of DPF3. To verify the function of LINC00982 in vivo, the BC cells were implanted in nude mice and the results suggested the tumor growth and malignant phenotype were suppressed by LINC00982. In this study, we prove that LINC00982 regulates the growth and development of BC through STAT3/NF-κB signal pathway, mediated by the miR-765/DPF3 axis. LINC00982 may function as a target molecule to take part in the prognosis and therapy of BC.
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JOURNAL OF NANOBIOTECHNOLOGY
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2022
·Vol. 20
·期 1
·1-14
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IF 9.429
Deng Bo,Ma Bing,Ma Yingying,Cao Pei,Leng Xigang,Huang Pengyu,Zhao Yuanyuan,Ji Tianjiao,Lu Xueguang,Liu Lanxia
Chemotherapeutics that can trigger immunogenic cell death (ICD) and release tumor-specific antigens are effective on treating a variety of cancers. The codelivery of chemotherapeutics with adjuvants is a promising strategy to achieve synergistic therapeutic effect. However, low drug loading and complicated preparation of current delivery systems lead to carrier-associated toxicity and immunogenicity. Herein, we developed a facile approach to construct liposomal spherical nucleic acids (SNA) by the self-assembly of 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine (DOPE)-doxorubicin conjugate and DOPE-matrix metalloproteinases-9 (MMP-9) responsive peptide-CpG conjugate (DOPE-MMP-CpG). Liposomal SNAs efficiently co-delivered DOX and CpG into tumors and released the two drugs upon biological stimuli of MMP-9 enzyme in tumor microenvironment (TME) and high concentration of endogenous glutathione in tumor cells. We demonstrated that liposomal SNA enhanced activation of dendritic cells (DCs), promoted expansion of CD8 + and CD4 + T cells in both tumors and spleen, inhibited tumor growth, and extended animal survival. This work provided a simple strategy of delivering chemotherapeutics and adjuvants to tumors with synergistic therapeutic effect and reduced side effect. Graphical Abstract
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危险品化学品经营许可证（带存储）