ANALYST
·
2020
·Vol. 145
·期 5
·1933-1942
·
IF 3.978
Xiuyun Wang,Jie Zhang,Yuanan Wei,Tianyu Xing,Tingting Cao,Shuo Wu,Fenghui Zhu
A simple and easy-operation electrode modification strategy was proposed using Cu-MOF/GO nanohybrids for physiologists and pathologists for the feasible and reliable simultaneous electrochemical detections of DNA bases, namely guanine and adenine. The nanohybrids were prepared via a simple ultrasonic method and were employed for the fabrication of a sensing interface. SEM, TEM, XRD, FT-IR, and electrochemical characterizations were used to characterize the general morphology and structure of the nonohybrids. The proposed Cu-MOF/ERGO/GCE exhibited ultra-stable and high-sensitivity performance in the simultaneous electrochemical detection of guanine and adenine. The recorded DPV curves revealed a linear increase in the faradaic signals with increase in the concentrations of guanine and adenine in the range of 0.02–10 µM and 20–100 µM for guanine, and 0.005–20 µM and 40–200 µM for adenine. The relative standard deviation of guanine and adenine for 50 consecutive detections is 1.37% and 1.92%, respectively. It was proved that the proposed Cu-MOF/ERGO/GCE can be performed for the detection of guanine and adenine in real samples, such as Herring sperm DNA, and satisfactory results were obtained. This strategy does not require complicated modification procedures, professional modification techniques, or sophisticated instruments, but it can provide a highly sensitive and stable detection method, which is expected to expand and deepen the applications of electrochemical detection in life science research.
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TALANTA
·
2022
·
IF 6.556
Jialin Zhang,Wenjing Liu,Jinhui Li,Kangqiang Lu,Herui Wen,Jiali Ren
The foodborne pathogens have caused many life-threatening diseases. Herein, a new electrochemical platform was developed for detection, using 3D walking machine and enzyme-free toehold-mediated strand displacement. High-variable region of 16S rDNA fragment of Escherichia coli ( E. coli ) was used as the target to verify the approach. In presence of target, Exonuclease III (Exo III)-powered hairpin-loaded Au nanoparticles ( [email protected] ) was aroused, which emerged large amounts of 3D toehold-loaded AuNPs ( [email protected] ) walking machine. The “legs” of the walking machine hybridized with hairpin2 (HP2) tracks probe modified on surface of electrode and exposed toehold region of HP2 probe. The toehold-mediated strand displacement triggered rolling of AuNPs along the electrode, through the interaction of hairpin3-F c (HP3-F c ) probe with toehold region of HP2 probe. Thus large amounts of F c were closed to the electrode and led to significant current responses for E. coli detection. The method could continuously recycle low-concentration targets. Furthermore, due to the increase of “legs” and decrease of the derailment of leg DNA , which was beneficial to shorten detection time and amplify response. The detection limits (LOD) was 20 CFU/mL, the detection time was less than 1.5 h. This method was promising to be applied in early diagnosis of foodborne pathogen.
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Biosensors-Basel
·
2023
·Vol. 13
·期 7
·723
·
IF 5.4
Fengli Gao,Gang Liu,Yishu Qiao,Xiuwen Dong,Lin Liu
This work reports the development of a fluorescence method for the detection of poly(ADP-ribose) polymerase-1 (PARP1), in which a phenylboronic acid-modified fluorescein isothiocyanate dye (FITC-PBA) was used to recognize the formed poly(ADP-ribose) (PAR) polymer. The detection system was designed by conjugating recombinant streptavidin (rSA) with PARP1-specific double-stranded DNA (dsDNA) through streptavidin–biotin interaction. Capture of PARP1 via rSA–biotin–dsDNA allowed for the poly-ADP-ribosylation (PARylation) of both rSA and PARP1 in a homogeneous solution. The resulting rSA–biotin–dsDNA/PAR conjugates were then captured and separated via the commercialized nitrilotriacetic acid–nickel ion-modified magnetic bead (MB-NTA-Ni) through the interaction between NTA–Ni on MB surface and oligohistidine (His6) tag in rSA. The PAR polymer could capture the dye of FITC-PBA through the borate ester interaction between the boronic acid moiety in PBA and thecis-diol group in ribose, thus causing a decrease in fluorescence signal. The PARylation of streptavidin and the influence of steric hindrance on PARylation efficiency were confirmed using reasonable detection strategies. The method showed a wide linear range (0.01~20 U) and a low detection limit (0.01 U). This work should be valuable for the development of novel biosensors for the detection of poly(ADP-ribose) polymerases and diol-containing species.Keywords:streptavidin;nitrilotriacetic acid;magnetic bead;poly(ADP-ribose) polymerase;boronic acid;immobilization-free
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APPLIED SPECTROSCOPY
·
2023
·
IF 3.5
Ning-Ning Wu,Lin-Ge Chen,Hai-Bo Wang
A sensitive fluorescent sensor has been developed for the determination of tetracycline (TC) using adenine thymine (AT)-rich single-stranded DNA (ssDNA) templated copper nanoclusters (CuNCs) as a fluorescent probe. Fluorescent ssDNA-CuNCs were synthesized by employing AT-rich ssDNA as templates and ascorbic acid as reducing agents through a facile one-step method. The as-prepared ssDNA-CuNCs exhibited strong fluorescence with a large Stokes shift (240 nm) and stable fluorescence emission. In the presence of TC, the fluorescent intensity of ssDNA-CuNCs was obviously decreased through the inner filter effect, due to the spectral overlapping between ssDNA-CuNCs and TC. Under the optimal conditions, the strategy exhibited sensitive detection of TC with a linear range from 2 nM to 30 μM and with a limit of detection of 0.5 nM. Furthermore, the sensor was successfully applied for the detection of TC in milk samples. Therefore, it provided a simple, rapid, and label-free fluorescent method for TC detection.
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Frontiers of Chemical Science and Engineering
·
2021
·Vol. 15
·期 6
·1572-1582
·
IF 4.204
Wu Shuyao,Sui Chengquan,Wang Chong,Wang Yulu,He Dongqing,Sun Ying,Zhang Yu,Meng Qingbo,Ma Tianyi,Song Xi-Ming
High density and uniform distribution of the gold nanoparticles functionalized single-stranded DNA modified reduced graphene oxide nanocomposites were obtained by non-covalent interaction. The positive gold nanoparticles prepared by phase inversion method exhibited good dimensional homogeneity and dispersibility, which could readily combine with single-stranded DNA modified reduced graphene oxide nanocomposites by electrostatic interactions. The modification of single-stranded DNA endowed the reduced graphene oxide with favorable biocompatibility and provided the preferable surface with negative charge for further assembling of gold nanoparticles to obtain gold nanoparticles/single-stranded DNA modified reduced graphene oxide nanocomposites with better conductivity, larger specific surface area, biocompatibility and electrocatalytic characteristics. The as-prepared nanocomposites were applied as substrates for the construction of cholesterol oxidase modified electrode and well realized the direct electron transfer between the enzyme and electrode. The modified gold nanoparticles could further catalyze the products of cholesterol oxidation catalyzed by cholesterol oxidase, which was beneficial to the enzyme-catalyzed reaction. The as-fabricated bioelectrode exhibited excellent electrocatalytic performance for the cholesterol with a linear range of 7.5–280.5 µmol·L −1 , a low detection limit of 2.1 µmol·L −1 , good stability and reproducibility. Moreover, the electrochemical biosensor showed good selectivity and acceptable accuracy for the detection of cholesterol in human serum samples.
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RSC Advances
·
2014
·Vol. 4
·期 57
·29968-29974
·
IF 3.708
Yongjuan Wang,Yuming Zhou,Tao Zhang,Man He,Xiaohai Bu
This work describes a facile approach for the delaminating of Ni–In-layered double hydroxides (LDHs) by the intercalation of acetate anions in the LDHs gallery using co-precipitation method. The acetate-intercalated Ni–In LDHs exhibited swelling behavior in water and delaminated into semitransparent suspensions on the action of water. The transmission electron microscopy (TEM) and atomic force microscopic (AFM) observations revealed the formation of unilamellar LDHs nanosheets. This simple method did not need reflux at high temperatures, overcoming the deficiency of using organic solvents, resulting in a steady colloidal suspension of 2D nanosheets. The unique properties of 2D ultrathin nanosheets with full access to the positively charged activity sites make them attractive in terms of the improvement of DNA adsorption. The effect of dosage on the adsorption of DNA and the adsorption isotherm were investigated in detail. The equilibrium data were found to be well described by both the Langmuir and Freundlich models. Based on the mechanism of adsorption, the adsorption sites of exfoliated nanosheets can be easily occupied by highly negatively charged small anions through electrostatic interactions. The adsorbed DNA can be released via the addition of a hydrogen phosphate solution.
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BIOSENSORS & BIOELECTRONICS
·
2025
·
IF 10.5
Mengzhu Zhang,Mei Han,Rui Su,Shouhua Feng
Herein, a biosensor was constructed based on ultra-small manganese dioxide (MnO 2 ) nanozyme-encapsulated aptameric DNA hydrogel (MnO 2 @DNA hydrogel) for the sensitive and portable detection of xanthine (XA). This DNA hydrogel was fabricated via complementary base pairing-mediated self-assembly. Upon the binding of XA to the aptamer, the aptamer undergoes a conformational change, which reduces cross-linking density of DNA hydrogel and thereby facilitates the release of pre-encapsulated MnO 2 nanozymes. These nanozymes exhibited oxidase-like activity, enabling the oxidation of ABTS into a measurable quantifiable signal. The biosensor demonstrated a linear detection range of 0.01-50 μM for XA, with a low limit of detection of 4.68 × 10 -3 μM. Notably, it showed excellent specificity toward interfering substances. More importantly, the proposed sensor exhibited reliable performance in biological samples, with recoveries ranging from 98.50% to 105.70%. and relative standard deviations below 6%. Smartphone-assisted detection, which quantified signals through extracted RGB/HSV parameters, correlated well with ultraviolet-visible spectroscopy results. Compared to ultraviolet-visible spectroscopy-based detection methodologies, the smartphone-assisted approach, while exhibiting marginally lower sensitivity, enabled rapid on-site testing. This platform thus provides a promising tool for ultra-sensitive detection of XA in clinical diagnostics and resource-constrained settings.
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ACS Omega
·
2025
·
IF 4.3
Na Du,Guochao Xu,Yao Zhang,Jing Hou,Ying Wang,Yiran Li,Bing Xu
Nosocomial infection is a major global cause of mortality, posing significant public health challenges. Recent studies have identified RNA biomarkers, including microRNA (miRNA) and circular RNA (circRNA), as promising diagnostic tools for nosocomial infection, providing essential information for timely clinical intervention. In this study, we developed a versatile and label-free circRNA and miRNA detection platform based on a Cas13a/crRNA complex-based target recognition that triggers a primer exchange reaction (PER)-driven DNA walker. In this method, the Cas13a/crRNA complex is used to specifically identify target RNA biomarkers and to induce subsequent signal amplification strategies following cleavage of uracil (UUU, trU)-containing locked “sensing probe” based on trans-cleavage of Cas13a. Taking the merit of the high specific target recognition capability of Cas13a/crRNA, false signals or unintended circRNA degradation can be greatly avoided. The PER performed on the surface of streptavidin-coated magnetic beads (sMBs) motivated the walking of primer sequences, greatly amplifying fluorescence signals. Based on this design, the proposed method exhibits high sensitivity and precision, with detection limits as low as 0.41 fM for miRNA-21 and 3.12 fM for cZNF292. These findings suggest that this strategy could advance the identification of RNA biomarkers and hold considerable potential for future applications in diagnosing nursing infections.
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JOURNAL OF INORGANIC BIOCHEMISTRY
·
2025
·
IF 3.2
Jia-yu Xu,Xue-bin Bi,Sha-sha Luo,Yue Huang,Chen-yu Wang,Chang-chun Wen,Rui-xue Liu,Yan-cheng Liu,Hong Liang
This research presents the design, synthesis, structural characterization, and evaluation of the anticancer activities of three new pyrimidylanthrahydrazone cobalt(II) complexes: 1 ) 9-MPMAH-Co, 2 ) 9-FPMAH-Co, and 3 ) 9-PMAH-Co. Single-crystal X-ray diffraction analysis confirmed that all three complexes adopt a hexacoordinate mononuclear geometry. However, differences in their coordination modes were observed due to variations in the ligand substituents (-CH 3 , -F, -H). Spectroscopic DNA interaction studies indicated that all three cobalt complexes exhibit varying levels of DNA intercalation. Topoisomerase I inhibition assays revealed that 9-PMAH-Co demonstrates significant enzyme inhibition at a low concentration of 1 μM. In vitro antiproliferative assays confirmed that 9-PMAH-Co exhibits potent cytotoxic activity against SK-OV-3 and HeLa-229 cancer cell lines, with IC₅₀ values of 4.99 ± 0.18 μM and 8.09 ± 1.13 μM, respectively, while showing reduced toxicity toward normal liver cells (HL-7702) compared to cisplatin. Further investigation through cell cycle analysis indicated that 9-PMAH-Co induces G2/M phase arrest in SK-OV-3 cells, with a population increase to 91.37 % (Δ = 76.59 %). Studies on the structural-activity relationship suggest that the synergistic interactions between the ligand substituents and the cobalt center play a crucial role in modulating biological activity, highlighting 9-PMAH-Co as a promising lead compound for the development of targeted anticancer agents.
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CHEMICAL ENGINEERING JOURNAL
·
2025
·
IF 13.2
Zhen Yu,Yan Zhang,Dongquan Leng,Tingting Wu,Si-Jia Ge,Dawei Fan,Hongmin Ma,Yu Du,Qin Wei
Human papillomavirus (HPV) infection represents a major global health concern due to its strong association with cervical cancer and other malignancies, underscoring the critical need for highly sensitive detection methods. In this study, an advanced photoelectrochemical biosensing platform was developed for ultrasensitive HPV detection. An In 2 S 3 -sensitized SnO 2 heterostructure substrate that dramatically enhances photogenerated charge separation efficiency, generating extremely significant photocurrent enhancement. Furthermore, a CuFe 2 O 4 @T-COF nanosphere serving as a multifunctional signal quencher through synergistic steric hindrance, competitive oxygen consumption and light absorption effects. Notably, an exonuclease III-powered DNA walker system enabling cyclic signal amplification through programmable target recycling. The biosensor demonstrates exceptional analytical performance, achieving a broad dynamic range of 10 −6 to 10 μmol/L with an ultralow detection limit of 1.37 × 10 −7 μmol/L. This breakthrough sensing platform not only provides a powerful tool for early HPV diagnosis but also opens new possibilities for developing next-generation biosensors with clinical-level sensitivity and specificity.
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Biosensors-Basel
·
2025
·Vol. 15
·期 11
·747
·
IF 5.6
Qianyu Yang,Zhiwei Liu,Xinrui Xu,Zihao Zhao,Ze Fan,Bin Du,Jianjie Xu,Jiwei Xu,Jiang Wang,Bing Liu,Xihui Mu,Zhaoyang Tong
Over the past few decades, Fc fragment-conjugated proteins, such as Protein A, have been extensively utilized across a range of applications, including antibody purification, site-specific immobilization of antibodies, and the development of biosensing platforms. In this study, building upon our group prior research, we designed and screened an affinity DNA functional ligand (A-DNAFL) and experimentally validated its binding affinity (KD= 6.59 × 10−8) toward mouse IgG antibodies, whose binding performance was comparable to that of protein A. Systematic evaluations were performed to assess the binding efficiency under varying pH levels and ionic strength conditions. Optimal antibody immobilization was achieved in PBST-B buffer under physiological pH 7.2–7.4 and containing approximately 154 mM Na+and 4 mM K+. Two competitive binding assays confirmed that the A-DNAFL binds to the Fc fragment of murine IgG antibody. Furthermore, molecular docking simulations were employed to investigate the interaction mode, revealing key residues involved in binding as well as the contributions of hydrogen bonding and hydrophobic interactions to complex stabilization. Leveraging these insights, A-DNAFL was utilized as a tool for oriented immobilization of antibodies on the sensing interface, enabling the construction of an immunosensor for ricin detection. Following optimization of immobilization parameters, the biosensor exhibited a detection limit of 30.5 ng/mL with the linear regression equation is lg(Response) = 0.329 lg(Cricin) − 2.027 (N= 9,R= 0.938,p< 0.001)—representing a 64-fold improvement compared to conventional protein A-based methods. The system demonstrated robust resistance to nonspecific interference. Sensing interface reusability was also evaluated, showing only 8.55% signal reduction after two regeneration cycles, indicating that glycine effectively elutes bound antibodies while preserving sensor activity. In summary, the A-DNAFL presented in this study represents a novel antibody-directed immobilization material that serves as a promising alternative to protein A. It offers several advantages, including high modifiability, low production cost, and a relatively small molecular weight. These features collectively contribute to its broad application potential in biosensing, antibody purification, and other areas of life science research.
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MICROCHEMICAL JOURNAL
·
2025
·
IF 5.1
Yan Tan,Zhipeng Wang,Peng Yu
Human papillomavirus type 16 (HPV16) is a significant biomarker for cervical cancer, and its precise detection is vital for diagnosing the disease. The work involved developing an electrochemical sensor by utilizing copper nanoclusters (CuNCs), which integrated triblock polyadenine probe (TPP) and hybridization chain reaction (HCR) for sensitively detecting HPV16 DNA. The TPP was assembled on the electrode owing to the strong affinity between its adenine bases and gold. The absence of HPV16 DNA allowed the capture probe to bind with the hairpin DNA, which triggered HCR and generated a large number of CuNCs, causing a substantial electrochemical impedance signal. However, when there was HPV16 DNA in the reaction system, it could bind specifically to the two flanking probes of TPP, causing the release of the capture probe. Without capture probe, the HCR was unable to be triggered, thus generating a small amount of CuNCs and resulting in a reduced electrochemical impedance signal. With the optimal conditions, the sensor could detect HPV16 DNA within a broad linear range of 1 × 10 −12 M – 1 × 10 −5 M and had a low detection limit of 2.6 × 10 −13 M. A new analytical platform for the sensitive detection of DNA was developed, which exhibited good sensitivity and selectivity.
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