Royal Society Open Science
·
2018
·Vol. 5
·期 8
·
IF 2.504
Zhenfei Huang,Zhihong Wu,Bupeng Ma,Lingjia Yu,Yu He,Derong Xu,Yuanhao Wu,Hai Wang,Guixing Qiu
Titanium (Ti) is an ideal bone substitute due to its superior bio-compatibility and remarkable corrosion resistance. However, in order to improve the osteoconduction and osteoinduction capacities in clinical applications, different kinds of surface modifications are typically applied to Ti alloys. In this study, we fabricated a tightly attached polydopamine-assisted Fe3O4 nanoparticle coating on Ti with magnetic properties, aiming to improve the osteogenesis of the Ti substrates. The PDA-assisted Fe3O4 nanoparticle coatings were characterized by scanning electron microscopy, energy dispersive spectroscopy, atomic force microscopy and water contact angle measurements. The cell attachment and proliferation rate of the human bone mesenchymal stem cells (hBMSCs) on the Ti surface significantly improved with the Fe3O4/PDA coating when compared with the pure Ti without a coating. Furthermore, the results of in vitro alkaline phosphatase (ALP) activity at 7 and 14 days and alizarin red S staining at 14 days showed that the Fe3O4/PDA coating on Ti promoted the osteogenic differentiation of hBMSCs. Moreover, hBMSCs co-cultured with the Fe3O4/PDA-coated Ti for approximately 14 days also exhibited a significantly higher mRNA expression level of ALP, osteocalcin and runt-related transcription factor-2 (RUNX2). Our in vitro results revealed that the present PDA-assisted Fe3O4 nanoparticle surface coating is an innovative method for Ti surface modification and shows great potential for clinical applications.
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PubMed
高影响因子
ACS Applied Bio Materials
·
2018
·
IF 0
Xiaohong He,Xue Shen,Dongming Li,Yiyao Liu,Kun Jia,Xiaobo Liu
Nanoparticles exhibiting good biocompatibility and multifunctional optical, magnetic, and reactive properties are essential materials for the construction of next generation theranostics platforms. The core–shell structured CdSe@CdS is one of few semiconductor quantum dots (QD) that shows ideal photoluminescence for biological application including unity quantum yields, identical photoluminescence for ensembles and single dot, nonblinking, and antibleaching. However, overcoming toxicity concerns from Cd2+ is still a great challenge for promoting the practical medical application of the CdSe@CdS QD. Besides, the high quality luminescent and superparamagnetic nanoparticles at present are basically hydrophobic, which implies that the phase transfer of these functional nanoparticles into aqueous phase is the primary step to enable their biomedical application. Herein, we have developed a facile protocol to fabricate highly biocompatible nanoparticles showing both modulated luminescent and magnetic properties via a one-step self-assembling of amphiphilic block copolyarylene ether nitriles (amPEN), oleic acid stabilized CdSe@CdS QD, and Fe3O4 superparamagnetic nanoparticles (SP) in microemulsion system. Benefiting from the aromatic backbone structure of amPEN and its strong hydrophobic interaction with surface capping agent of QD/SP, the fabricated hybrid nanoprobe exhibits quite competitive colloids stability as well as fluorescent/magnetic properties, which ensures its application for in vitro fluorescence and magnetic resonance (MR) imaging of cancer cells.
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PubMed
BIOCHEMICAL ENGINEERING JOURNAL
·
2019
·
IF 3.371
Jianguo Zhang,Hongli Mao,Mengla Li,Erzheng Su
An enzymatic approach to β-cyclodextrin synthesis attracts the attention of food engineers and chemists because of the broad applications of β-cyclodextrin in the food industry and the demand for a green process for β-cyclodextrin production. Achieving a high recovery of cyclodextrin glycosyltransferase through immobilization and a high proportion of β-cyclodextrin in a product mixture of cyclodextrins would improve the purification process of β-cyclodextrin. In this study, a recombinant cyclodextrin glycosyltransferase (CGTase) was immobilized on polydopamine-Fe 3 O 4 (PDA-Fe 3 O 4 ) nanoparticles in the presence of polyethyleneimine (PEI). After optimization of the preparation of the PDA-Fe 3 O 4 nanoparticles and the CGTase immobilization step, 77.9% of the CGTase was recovered. The immobilized CGTase exhibited good thermal and operational stabilities, and gave a ratio of 88.9% β-cyclodextrin when the reaction temperature and kinetic parameters were optimized. Immobilized CGTase was recycled nine times and retained 19.1% β-activity and 0% γ-activity. This study has established a reliable approach to the high recovery of CGTase during efficient β-cyclodextrin production.
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高影响因子
BIOSENSORS & BIOELECTRONICS
·
2018
·
IF 8.173
Wenshi Li,Gao-Chao Fan,Fengxian Gao,Yige Cui,Wei Wang,Xiliang Luo
Sensitive but with simple, inexpensive detection of disease-related biomarkers in real biological samples is of quite necessity for early diagnosis and disease surveillance. We herein first introduced high-activity Fe 3 O 4 nanozyme as signal amplifier to develop an ultrasensitive photoelectrochemical (PEC) immunoassay , which meanwhile has the distinct merits of both simplicity and low cost compared with previously reported enzyme-labeling PEC immunoassays. In the proposal, to illustrate and describe the PEC platform, prostate-specific antigen (PSA, Ag) was used as a target model. Specifically, ZnO nanorods (ZnO-NRs) grown vertically on a bare indium–tin oxide (ITO) electrode was deposited with ZnIn 2 S 4 nanocrystals , producing ZnIn 2 S 4 /ZnO-NRs/ITO photoelectrode as the PEC matrix to modify capture PSA antibody (Ab 1 ). Histidine-modified Fe 3 O 4 (his-Fe 3 O 4 ) nanozyme as signal amplifier was linked with signal PSA antibody (Ab 2 ) to form his-Fe 3 O 4 @Ab 2 conjugate, and was anchored through specific sandwich immunoreaction. The labeling his-Fe 3 O 4 nanozyme acted as a peroxidase to induce the generation of the insoluble and insulating precipitation, resulting in an evident decrease in the photocurrent signal. On account of combined effects of high catalytic efficiency of the his-Fe 3 O 4 nanozyme and excellent PEC properties of the ZnIn 2 S 4 /ZnO-NRs/ITO photoelectrode, ultralow detection limit of 18 fg/mL for target Ag detection was achieved. Besides, as high-activity his-Fe 3 O 4 nanozyme has substituted natural enzyme as signal amplifier, simplicity and low cost of the PEC immunoassay was realized.
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高影响因子
Journal of Nano Research
·
2018
·
IF 0.665
Wen Ting Liang,Dan Li,Xue Wen Ma,Wen Juan Dong,Jing Li,Rui Fang Wu,Chuan Dong,Qing Chen Dong
Surface β-cyclodextrin polymer coated Fe3O4 magnetic nanoparticles (NPs) were prepared by anchoring pyromellitic dianhydride derivate-b-cyclodextrin polymer (b-CD-P) onto the surface of Fe3O4 magnetic NPs, which is generated in situ through a co-precipitation method. The transmission electron microscopy (TEM) result indicates that Fe3O4@β-CD-P NPs have an effective average size of 15 ± 2 nm. The surface coated layer β-CD-P was confirmed by Fourier-Transformed Infrared Spectroscopy, and the amount of which was determined to be 138.2 mg g-1 by thermogravimetric analysis. To explore the potential application of such nanocomposites for organic pollutants adsorption, malachite green (MG), a typical cancer-causing water pollutant was evaluated by spectrophotometric method. It was finally learnt that, the adsorption rate of MG by Fe3O4@β-CD-P NPs follows pseudo-second-order kinetics with adsorption isotherm fitted by the Langmuir isotherm model well. The maximum adsorption capacity was measured to be 88.49 mg g-1 at 25 °C. Additionally, a good recyclability of the Fe3O4@β-CD-P was observed over four usage cycles, with slight decrease of adsorption capability.
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高影响因子
ADVANCED FUNCTIONAL MATERIALS
·
2018
·Vol. 28
·期 20
·1707440
·
IF 13.325
Siming Yu,Guowei Li,Rui Liu,Dong Ma,Wei Xue
Nowadays, antibiotic abuse increases the emergence of multidrug-resistant bacterial strains, which is the major reason for the failure of conventional antibiotic therapies. Therefore, developing novel antibacterial materials or therapies is an urgent demand. In the present study, photothermal and NO-releasing properties are integrated into a single nanocomposite to realize more efficient bactericidal effects. To this end, polydopamine (PDA) coated iron oxide nanocomposite (Fe 3 O 4 @PDA) is used as a photoconversion agent and the core, first three generation dendritic poly(amidoamine) (PAMAM-G3) is grafted on the surface of Fe 3 O 4 @PDA, and subsequently NO is loaded with the formation of NONOate. The resultant Fe 3 O 4 @PDA@PAMAM@NONOate displays controllable NO release property under intermittent 808 nm laser irradiation and excellent bacteria-separation efficiency. Moreover, excellent synergistic photothermal and NO antibacterial effects are observed against both Gram-negative Escherichia coli and Gram-positive Staphylococcus aureus , where bacterial viability and biofilm are significantly reduced. An antibacterial mechanism study reveals that the materials first adsorb onto the bacterial membrane, then cause damage to the membrane by the increased local temperature and the released NO under laser irradiation conditions, finally leak the intracellular components like DNA and induce bacteria death. The work provides a novel way for designing of antibacterial materials with higher efficiency.
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高影响因子
Journal of Materials Chemistry B
·
2014
·Vol. 3
·期 6
·1010-1019
·
IF 0
Chunyu Yang,Wei Guo,Liru Cui,Na An,Ting Zhang,Gang Guo,Huiming Lin,Fengyu Qu
Multifunctional nanocarriers based on the magnetic Fe3O4 nanoparticle core and bis-(3-carboxy-4-hydroxy phenyl) disulfide (R–S–S–R1) modified mesoporous silica shell (Fe3O4@mSiO2@R–S–S–R1) were synthesized for cancer treatment through passive targeting and enzyme-sensitive drug release. Anti-cancer drug doxorubicin (DOX) was used as the model cargo to reveal the release behavior of the system. The drug loading system (DOX–Fe3O4@mSiO2@R–S–S–R1) retains the drug until it reaches the tumor tissue where glutathione reductase (GSH) can degrade the disulfide bonds and release the drug. Furthermore, the grafting amount of R–S–S–R1 can be used to adjust the release performance. All the release behaviors fit the Higuchi model very well and the release kinetics are predominated by disulfide bond degradation and mesoporous structure. With good bioactivity and targeted release performance, the system could play an important role in the development of intracellular delivery nanodevices for cancer therapy.
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PubMed
Materials
·
2013
·Vol. 6
·期 12
·5690-5699
·
IF 2.247
Zhiyong Wang,Yanyan Bai,Wenchao Wei,Ning Xia,Yuhui Du
In this work, we designed a visual biosensor for dopamine (DA) detection using magnetic Fe3O4particles and dithiobis(sulfosuccinimidylpropionate)-modified gold nanoparticles (DTSSP-AuNPs) as the recognition elements. Specifically, DA molecules were assembled onto the surface of DTSSP-AuNPs via the amine coupling reaction between the amino group of DA and activated carboxyl group of DTSSP. Accordingly, DA-anchored DTSSP-AuNPs were captured by Fe3O4through the interaction of catechol and iron. In a magnetic field, the formed Fe3O4-DA-DTSSP-AuNPs conjugates were easily removed from the solution, leading to fading of the AuNPs suspension and decrease of the UV/Vis signal. As a result, a detection limit of 10 nM for DA was achieved. The theoretical simplicity and high selectivity demonstrated that the sandwich-type strategy based on Fe3O4and AuNPs would lead to many colorimetric detection applications in clinical study by rationally designing the surface chemistry of AuNPs and Fe3O4.Keywords:gold nanoparticles;magnetic particle;biosensor;dopamine;colorimetric assay
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高影响因子
BIOCHEMICAL ENGINEERING JOURNAL
·
2015
·
IF 2.467
Dong Yang,Xueyan Wang,Jiafu Shi,Xiaoli Wang,Shaohua Zhang,Pingping Han,Zhongyi Jiang
Herein, the reduced graphene oxide–Fe 3 O 4 (rGO–Fe 3 O 4 ) nanocomposites are synthesized by the simultaneous reduction of graphene oxide (GO) and in situ deposition of Fe 3 O 4 nanoparticles ( ca. 20 nm) enabled by Fe 2+ ions. The rGO–Fe 3 O 4 nanocomposites integrate the magnetic property of Fe 3 O 4 nanoparticles and the large specific surface area of rGO nanosheets. Catalase (CAT), as a commonly used enzyme, can be efficiently immobilized on the rGO–Fe 3 O 4 nanocomposites through physical adsorption. The CAT loading capacity is as high as 312.5 ± 12.6 mg g −1 , while the activity recovery of CAT can be high up to nearly 98%. The strong magnetic response of immobilized CAT ensures its easy separation from the reaction system when an external magnetic field is applied. Owing to the hydrophobic and hydrogen bonding interactions between enzyme and the support, the immobilized CAT exhibits zero leaching, desirable stability and excellent reusability.
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高影响因子
Journal of Nanomaterials
·
2015
·
IF 1.644
Song Yang,Tian Zeng,Yaling Li,Jun Liu,Qian Chen,Ji Zhou,Yong Ye,Bin Tang
The fast detection and removal of organic dyes from contaminated water has become an urgent environmental issue due to their high toxicity, chemical stability, and low biodegradability. In this paper, we have developed graphene oxide decorated Fe3O4@SiO2 (Fe3O4@SiO2-GO) as a novel adsorbent aiming at the rapid adsorption and trace analysis of organic dyes followed by surface enhanced Raman scattering (SERS). The structure and morphology of the nanocomposites were characterized by transmission electron microscopy (TEM), Fourier infrared spectrometry (FT-IR), X-ray diffraction (XRD), and vibrating sample magnetometer (VSM). The obtained nanocomposites were used to adsorb methylene blue (MB) in aqueous solution based on π-π stacking interaction and electrostatic attraction between MB and GO, and the adsorption behaviors of MB were investigated. Moreover, the obtained nanocomposites with adsorbed dyes were separated from the solution and loaded with silver nanoparticles for SERS detection. These nanocomposites showed superior SERS sensitivity and the lowest detectable concentration was 1.0 × 10−7 M.
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高影响因子
APPLIED SURFACE SCIENCE
·
2014
·
IF 2.538
Xiaofang Shen,Qin Wang,WenLing Chen,Yuehong Pang
Cysteine functionalized Fe 3 O 4 magnetic nanoparticles (Cys-Fe 3 O 4 MNPs) were prepared facilely for Hg(II) removal from aqueous solutions. Using Fe 2+ as precursors, air as oxidant and Cys as protectant, this novel material was one-pot synthesis at room temperature by oxidation–precipitation method with the assistance of sonication. The MNPs were characterized by TEM, VSM, FTIR, X-ray powder diffraction analysis (XRD) and TGA methods. Under the optimum experimental conditions, the removal efficiency was as high as 95% and the maximum sorption capacity is found to be 380 mg/mol for Hg(II). Study on adsorption kinetics shows that adsorption of Hg(II) onto Cys-Fe 3 O 4 MNPs follows pseudo-first-order kinetic model and the adsorption rate constant was 0.22 min −1 . Additionally, the Hg(II)-loaded Cys-Fe 3 O 4 MNPs could be easily regenerated up to 95% using 1.0 M acetic acid. These results indicated that Cys-Fe 3 O 4 MNPs is a potentially attractive material for the removal of Hg(II) from water.
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高影响因子
RSC Advances
·
2014
·Vol. 4
·期 86
·45920-45929
·
IF 3.708
Lisha Tan,Jiang Xu,Xiaoqin Xue,Zimo Lou,Jin Zhu,Shams Ali Baig,Xinhua Xu
Silica-coated magnetite (Fe3O4@SiO2) nanoparticles functionalized with amino, imino and sulfonic groups (Fe3O4@SiO2–mPD/SP) were successfully synthesized via a facile chemical oxidative polymerization of m-phenylenediamine (mPD) and m-sulfophenylenediamine-4-sulfonic acid (SP) monomers, and utilized for selective removal of Pb(II) and Cr(VI) from aqueous solutions. It was revealed by the characterizations that the polymers formed on Fe3O4@SiO2 nanoparticles were the true copolymers with a mPD–SP unit, rather than a mixture of mPD and SP homopolymers. Fe3O4@SiO2–mPD/SP nanocomposites could be easily separated from aqueous solutions within 30 s. The maximum adsorption capacities of Pb(II) (83.23 mg g−1) and Cr(VI) (119.06 mg g−1) on Fe3O4@SiO2–mPD/SP nanocomposites were obtained at the mPD/SP molar ratios of 95 : 5 and 50 : 50, respectively. Moreover, satisfactory selective removal of Pb(II) and Cr(VI) from their mixtures with Cu(II) and Ni(II) ions were exhibited by the Fe3O4@SiO2–mPD/SP (95 : 5) and Fe3O4@SiO2–mPD/SP (50 : 50), respectively. The Pb(II) adsorption equilibrium was reached within 5 min by Fe3O4@SiO2–mPD/SP (95 : 5). The adsorption data of Pb(II) and Cr(VI) were both fitted well to the Freundlich isotherm and followed the pseudo-second-order kinetic model. The adsorption mechanism of Pb(II) and Cr(VI) on Fe3O4@SiO2–mPD/SP nanocomposites included five processes, namely: ion-exchange, complexation adsorption, reduction reaction, electrostatic attraction and physical adsorption. The enhanced adsorption performance of nanoparticle-based magnetic adsorbents for selective removal of heavy metal ions can be achieved with such a copolymerization strategy.
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高影响因子
危险品化学品经营许可证（带存储）