MOLECULES
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2026
·Vol. 31
·期 15
·2648
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IF 5.1
Lixia Yuan,Yanqing Zhang,Huimin Liu,Xiaowei Lu,Yue Wang,Zhining Yan,Min Liu
Advanced glycation end products (AGEs) are implicated in the pathogenesis of chronic diseases. This study investigated the inhibitory effect of theaflavin (TA) on AGE formation in a human serum albumin (HSA)-fructose model system, both in the absence and presence of non-cytotoxic Mg2+or Mn2+. The underlying mechanism was elucidated using multiple spectroscopic techniques and molecular docking. TA significantly inhibited AGE formation, with the enhancement by metal ions following the order: 1.5 μM Mn2+> 1.5 μM Mg2+> 1 mM Mg2+. This trend aligned with the binding affinity between HSA and TA derived from molecular interaction studies. Site marker competition and docking results revealed that TA binds preferentially within subdomain IIA of HSA. Furthermore, the conformational changes in HSA following glycation and inhibition were monitored, along with the influence of metal ions on the antioxidant activity of TA. By integrating the results, it was concluded that free radical scavenging contributes more significantly to AGE inhibition than does blocking the glycation sites on HSA. Overall, this study elucidates the influence of metal ions on the inhibitory effect of TA against AGE formation and the corresponding mechanism, providing insights for the prevention and management of chronic diseases.
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Acta Pharmaceutica Sinica B
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2026
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IF 14.6
Jingjing Shi,Junyu Zhang,Xuting Zhou,Dandan Zhang,Hui Qi,Simian Chen,Yunlong Hou,Zhenhua Jia,Caisheng Wu
Comprehensive identification of in vivo metabolites from natural medicines (NMs) remains challenging due to their chemical complexity and the limited reference spectra. Here, we presented the deep mass dataset relevance bridging (Deep MDRB) platform, an integrated platform powered by a diagnostic weighted cosine similarity (DWCS) algorithm that efficiently facilitates high-coverage annotation of in vivo metabolites. By integrating mass spectral databases with biosynthetic and metabolic reaction rules, Deep MDRB enables fully automated non-targeted analysis, including peak extraction, background correction, multimodal data alignment, multilevel similarity matching, and parent–metabolite network bridging. As a proof of concept, Deep MDRB was applied to in vitro liver microsome models of verapamil and berberine, as well as an in vivo metabolic study involving mixed reference standards (MRS) and Tongxinluo (TXL) capsule in rats. The platform identified 1565 parent compounds with a 94.4% identification rate for the TXL capsule and constructed a biotransformation network of 29 in vitro , 83 (MRS) and 2069 (TXL capsule) in vivo metabolite nodes, respectively, with a correlation rate exceeding 90%. Compared to existing software, Deep MDRB substantially improves annotation coverage and network integrity. Overall, the “knowledge-data” dual-driven framework of Deep MDRB provides a novel strategy for metabolite profiling in complex systems.
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NUTRITION RESEARCH
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2026
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IF 3.6
Ningli Ye,Minghui Yu,Haixin Ju,Yuanyan Jiang,Yumeng Zhang,Lei Sun,Xiaohui Wu,Jing Chen,Hong Xu,Qian Shen
Imbalance of maternal diets can impair offspring development and contribute to congenital anomalies of the kidney and urinary tract (CAKUT). Excess maternal methionine intake is known to impair fetal development. The placenta serves as a crucial interface for maternal–fetal exchange and may contribute to these adverse outcomes. We hypothesize that excess methionine intake during pregnancy in mice increases the incidence of CAKUT in offspring. Therefore, we established a mouse model of 10% (w/w) methionine intake during pregnancy from Embryonic Day 8.5 to11.5 (E8.5-E11.5) and assessed the kidney phenotype. Crucial kidney developmental pathways were analyzed by RT–qPCR and immunofluorescence at E11.5. Changes in methionine metabolism in maternal plasma and placental tissues at E11.5 were analyzed using liquid chromatography–tandem mass spectrometry, and placental RNA–seq was performed. Offspring from dams exposed to excess methionine exhibited increased incidence of CAKUT (relative risk = 4.15, 95% confidence interval: 1.40-12.34, P = .010), primarily presenting as hydronephrosis and/or duplex collecting system. E11.5 embryonic kidneys showed shorter common nephric duct and decreased activity of the Gdnf/Ret/p-Akt pathway. At E11.5, homocysteine was elevated in the placentas, together with decreased mRNA expression of key metabolic enzymes betaine-homocysteine S-methyltransferase (Bhmt) and cystathionine γ-lyase (Cth) in the placentas. Concurrently, RNA-seq of E11.5 placentas revealed alterations in immune-inflammatory and vascular-related pathways. Overall, excessive maternal methionine intake in mice increased the incidence of CAKUT in offspring, which may be associated with altered activity of the Gdnf/Ret/PI3K/p-Akt pathway in embryonic kidneys as well as elevated placental homocysteine.
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COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
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2026
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IF 5.4
Yulan Yin,Qianmin Tu,Yuxin Bu,Sinan Feng,Huiting Cai,Qianqian Xu,Ruiyun You,Jing Zhuang,Yudong Lu,Jingbo Chen
Camellia oleifera oil (CO) emulsified by cationic cellulose nanofibrils. • Dual stabilization of CO via emulsion interface and gel network. • High-efficiency encapsulation and sustained release of CO by composite system. • Hydrogel retains strong antioxidant activity after 35 days of storage. In recent years, the skincare concept of nourishing skin with oils has attracted extensive attention. Camellia oleifera oil (CO) is a natural woody skincare oil with both skin barrier repair and antioxidant properties. To overcome the instability of its bioactive components, this study adopts a gel matrix composed of polyvinyl alcohol (PVA), tremella fuciformis polysaccharide (TFP) and polydopamine (PDA), loaded with CO Pickering emulsions stabilized by cationic cellulose nanofibrils (CCNF). By combining the oil-in-water emulsification method with a cyclic freeze-thaw strategy, a dual-stabilization structure of emulsion encapsulation and hydrogel network was constructed. The resultant hydrogel integrates excellent dynamic adhesion and mechanical properties, with a water holding capacity of 93.21% and a CO encapsulation efficiency of 80.38%. It enables long-term sustained release of CO for 120 h, and the release behavior follows the Fickian diffusion mechanism. It achieves DPPH and ABTS free radical scavenging ratio of 88.1% and 97.14%, respectively, with no significant attenuation of antioxidant activity during the 35 days storage period. The hydrogel also possesses favorable bacteriostatic activity and biocompatibility, with a hemolysis ratio below 5%, no skin irritation and no in vivo toxicity. This study provides a novel approach for the protection and delivery of CO as well as the green design of skincare hydrogels. Download: Download high-res image (196KB) Download: Download full-size image
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Advanced Science
·
2026
·
IF 14.1
Kailang Liu,Haocheng Ran,Haohui Ou,Peiying Chen,Cheng Qi,Tiantian Kong,Zhou Liu
Coacervate droplets formed via liquid–liquid phase separation offer unique opportunities as microreactors and delivery vehicles due to their ability to selectively concentrate biomolecules and support biochemical reactions. However, their passive nature severely restricts precise spatial and temporal control, posing barriers to practical implementation. Here, we report magneto-coacervate droplets constructed from gelatin, poly(diallyldimethylammonium chloride) (PDDA), and superparamagnetic Fe 3 O 4 @SiO 2 nanoparticles. These magneto-coacervate droplets exhibit reversible sol–gel transitions controlled by temperature and pH, tunable surface charge properties for electrostatically driven molecular enrichment, and precise three-dimensional manipulation under external magnetic fields. These magneto-coacervate droplets can serve as multifunctional platforms, greatly increasing the practical use of phase-separated microreactors in environmental remediation, biochemical processing, and targeted biomedical interventions. Demonstrated applications include efficient capture and recycling of microplastic pollutants, magnetically enhanced catalytic enzyme cascades with reaction rates 2–3 times higher than those of conventional methods, and targeted vascular embolization through controlled in situ gelation.
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APPLIED SURFACE SCIENCE
·
2026
·
IF 6.6
Jihang Zhang,Yihui Luo,Siwen Ji,Jinming Zhang,Xiang Chen,Lei Ge,Jinglan Wu,Zhuotao Tan,Sheng Xu,Bin Wu,Hanjie Ying,Wei Zhuang
Sequential coupling of hydrophobic orientation and rapid covalent locking. • Immobilized TLL exhibits >4000% relative activity via conformational tuning. • Interfacial microenvironment regulates partitioning and methanol tolerance. • Biocatalyst demonstrates exceptional durability and efficient conversion. Efficient multiphase bioprocessing for sustainable lipid valorization and biodiesel synthesis relies on robust solid–liquid biointerfaces. Their rational design is fundamentally constrained by severe reaction–diffusion constraints and the intrinsic activity–stability trade-off. Herein, we report a synergistic “orient-and-lock” strategy on bifunctional mesoporous SBA-15 to decouple these limitations. Hydrophobic domains first drive the oriented adsorption and “lid-opening” hyperactivation of Thermomyces lanuginosus lipase, followed by rapid isocyanide-mediated covalent anchoring to kinetically trap this metastable state without compromising structural flexibility. Molecular dynamics simulations elucidate a critical microenvironmental compartmentalization effect. This spatial partitioning selectively enriches lipophilic substrates while isolating polar inhibitors through localized hydrogen-bond networks and hydrophobic compartments, thereby effectively reducing substrate transfer resistance and minimizing active site blockage. Consequently, the immobilized biocatalyst exhibits an unprecedented 40-fold enhancement in apparent specific activity and a 57-fold increase in catalytic efficiency ( k cat / K m ) compared to the free enzyme. Its excellent tolerance to methanol enables a highly efficient one-step methanol addition reaction. The system demonstrates exceptional operational robustness, retaining 80% activity over 25 consecutive cycles and achieving a 96.8% biodiesel yield, establishing a synergistic paradigm of conformational engineering and transport regulation for high-performance heterogeneous biocatalysts. Download: Download high-res image (141KB) Download: Download full-size image
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ANALYTICA CHIMICA ACTA
·
2026
·
IF 6.1
Haoyuan Deng,Shan Li,Chunfei Bao,Daoyuan Zhao,Yubin Ding
Background Although sensor arrays are powerful for discriminating glycosaminoglycans, the construction of a sensor array is usually cost and labour inefficient. With the purpose to acquire a high dimensional response data using only a limited number of dyes, the rational assembly of fluorescent dye molecules may provide an alternative approach. We envision that co-assembling different dyes within a supramolecular structure can trigger multiple photophysical processes, which should produce high dimensional optical signals. Results A supramolecular AIEgen-porphyrin co-assembly is synthesized by assembling a negatively charged AIEgen ( TPE ) and meso-tetra(4-carboxyphenyl)porphine ( TCPP ) on the surface of a cationic polymer saying poly(diallyldimethylammonium chloride) ( PDDA ). With the co-assembly strategy, the aggregation-induced emission of TPE , the aggregation-caused quenching characteristic of TCPP , and especially, the Förster Resonance Energy Transfer efficiency from TPE to TCPP can be manipulated by a dynamic dye assembly approach. A sensor array is thus readily constructed by varying the loading ratio of TPE and TCPP on the surface of PDDA . Significance The sensor array is determined to be powerful for pattern recognition of various glycosaminoglycans even in 100% serum media through competitive compaction or removal of the aggregated TPE and TCPP dyes on the PDDA surface. It can be successfully applied for accurate and reliable detecting trace GAG analogues in Hep samples and identifying unknown GAG samples with an accuracy of 96.88%.
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ADVANCED MATERIALS
·
2026
·
IF 29.1
Wei Huang,Yi-Hui Zhang,Ming-Yu Chen,Jian-Hang Zhang,Lei Qiao,Zong-Huan Ba,Hong-Jie Gao,Jing Zang,Xuan Liu,Wen-Qian Liu,Ye-Zi You,Long-Hai Wang
Stem cell-based therapies hold substantial promise for the treatment of chronic wounds. However, limited clinical efficacy has frequently arisen not from inadequate cell potency, but from failure of current delivery paradigms to sustain cell survival and function within hostile host microenvironments. Here, we report a wound-adaptive, self-oxygenating stem cell encapsulation platform designed to overcome this delivery paradigm failure. The device embeds living stem cells within an immunoisolating hydrogel matrix conformally shaped to individual wound geometries and converts locally accumulated CO 2 into molecular O 2 , thereby providing an oxygen supply that preserves cellular metabolic activity in situ. Importantly, oxygenation in this system functions primarily to sustain cell viability rather than to directly oxygenate host tissue. Compared with conventional local or systemic delivery strategies, encapsulated cells exhibit markedly enhanced survival, persistence, and therapeutic function. Evaluation in diabetic rat and porcine wound models demonstrates accelerated resolution of inflammation, improved vascular maturation, and robust tissue regeneration. By reframing cell therapy as a problem of environment-cell compatibility, this platform establishes a clinically translatable delivery paradigm for precision stem cell therapy in chronic wound repair.
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Advanced Science
·
2026
·
IF 14.1
Kaiyuan Jiang,Fen Xiong,Yiwen Peng,Lingfei Meng,Xinran Wang,Yingruo Xu,Tian Tang,Hongchang Gao
Diabetic cardiomyopathy (DCM) is a major cardiovascular complication of diabetes with limited effective interventions. Using a streptozotocin-induced insulin-deficient, type 1 diabetes-like DCM mouse model, we show that intermittent fasting (IF) improves cardiac function and attenuates myocardial remodeling. Antibiotic-mediated microbiota depletion largely abolished these benefits, whereas fecal microbiota transplantation from IF-treated donors recapitulated cardioprotection, supporting a causal role of the gut microbiota. Metagenomic profiling identified Akkermansia muciniphila ( A. muciniphila ) as a prominent IF-responsive taxon, and A. muciniphila supplementation alleviated cardiac injury without obvious improvement in glycaemia. Integrated serum and heart metabolomics identified 1-methyl-L-histidine as a microbiota-associated metabolite reduced in diabetes but restored by IF and A. muciniphila . In vitro and ex vivo assays further supported an L-anserine-linked microbial route for 1-methyl-L-histidine generation. Importantly, oral 1-methyl-L-histidine supplementation recapitulated key cardioprotective effects, remodeled cardiac lipid homeostasis, and reduced lipid peroxidation and oxidative injury. Together, these findings support a gut microbiota–metabolite–lipid axis associated with IF-related cardioprotection in DCM and highlight microbial metabolites as tractable targets to complement dietary intervention.
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SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
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2026
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IF 4.8
Lewen Zhang,Zhibin Xu,Hanyang Wang,Hongzheng Chang,Guosheng Zhang,Zimu Li
To meet the demand for high-sensitivity ammonia (NH 3 ) detection at room temperature, this study develops an ultra-sensitive gas sensor by integrating the exceptional sensing properties of 2D MXene with the evanescent field advantages of optical microfibers. A carboxylated MXene-functionalized microfiber sensor was designed and fabricated. Density functional theory (DFT) calculations confirmed that carboxylation significantly enhances the adsorption energy between the MXene surface and NH 3 molecules compared to pristine MXene, theoretically validating its superior sensitivity. Experimentally, a uniform and robust carboxylated MXene thin film was coated onto the microfiber, demonstrating excellent mechanical stability for practical sensing. Gas sensing tests show a strong linear response within the 10–250 ppm range. Notably, the sensitivity is an order of magnitude higher than previously reported microfiber sensors, with a practical detection limit of 10 ppm and a theoretical limit as low as 6.90 ppm. Furthermore, the sensor exhibits excellent long-term stability over a 10-day measurement and gas selectivity. This research provides a new strategy for developing high-performance room-temperature gas sensors with significant potential for environmental atmospheric monitoring.
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Journal of Energy Storage
·
2026
·
IF 10.7
Huimin Feng,Xinxin Pi,Yanhui Li,Yunkai Wang,Mengjin Wen,Chengrui Wang,Chunlei Zhang,Yan He
The improvement of rechargeable zinc-air batteries is still limited by the sluggish oxygen electrochemistry at the air cathode, where both the oxygen reduction and oxygen evolution processes need to be accelerated. In this work, a Ni/Ni 3 C composite was obtained by thermal treatment using ammonia-activated carbon nanotubes as the conductive backbone and coal tar pitch as a supplementary carbon precursor. Ni and Ni 3 C nanoparticles are anchored on the nanotube framework, and part of their surface is wrapped by graphitized carbon. Such a multiphase architecture builds efficient electron-transport channels, reinforces the coupling between the Ni-based species and the carbon matrix, and improves structural robustness under electrochemical conditions. The catalyst therefore shows strong bifunctional oxygen electrocatalytic activity, giving a half-wave potential of 0.86 V for ORR and an overpotential of 250 mV at 10 mA·cm −2 for OER, together with satisfactory durability. When assembled as the air cathode of a rechargeable zinc-air battery, it affords an open-circuit voltage of about 1.42 V, a peak power density of 195 mW·cm −2 , and stable cycling for more than 200 h at 5 mA·cm −2 . DFT calculations indicate that nitrogen-containing interfacial sites reshape the local charge distribution and improve the adsorption and conversion of oxygen intermediates, which is favorable for both ORR and OER.
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JOURNAL OF APPLIED POLYMER SCIENCE
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2026
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IF 3.1
Zhilong Zhu,Hongxiang Ou,Honglai Xue,Fang Zhu
To enhance the oil–water separation performance and stability of melamine sponge (MS), stearic acid-grafted attapulgite (SA-g-OATP) was synthesized via silanization and amidation grafting. The hydrophobic sponge (MS-POS) was fabricated by dip-coating MS in a polydimethylsiloxane (PDMS) solution containing SA-g-OATP. Characterization confirmed a grafting rate of 20.73%. The synergy between SA-g-OATP and PDMS constructed a hierarchical “micron skeleton-nanoparticle” rough structure. The optimal sample, MS-POS1-1, exhibited superhydrophobicity with a water contact angle of 159.34° and achieved high adsorption capacities for oils and solvents, reaching 112.33 g/g for palm oil. In gravity-driven tests, it showed separation efficiencies above 98.34% for oil–water mixtures and effectively separated water-in-oil emulsions. The sponge demonstrated robust stability, retaining hydrophobicity after 40 cycles of tape-peeling or sandpaper abrasion, and withstanding prolonged immersion in pH 2–12 solutions. The adsorption capacity remained above 89.70% after 20 consecutive adsorption-extrusion cycles. Developed from low-cost materials via a simple process, MS-POS exhibits high adsorption capacity, excellent selectivity, and robust stability, showing significant potential for oily wastewater remediation.
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