Scientific Reports
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2024
·Vol. 14
·期 1
·1-9
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IF 3.8
Yuan Fang-Fang,Wang Pei,Han Xiao-Jie,Qin Ting-Ting,Lu Xin,Bai Hai-Jiao
The microreactor with two types of immobilized enzymes, exhibiting excellent orthogonal performance, represents an effective approach to counteract the reduced digestion efficiency resulting from the absence of a single enzyme cleavage site, thereby impacting protein identification. In this study, we developed a hydrophilic dual-enzyme microreactor characterized by rapid mass transfer and superior enzymatic activity. Initially, we selected KIT-6 molecular sieve as the carrier for the dual-IMER due to its three-dimensional network pore structure. Modification involved co-deposition of polyethyleneimine (PEI) and acrylamide (AM) as amine donors, along with dopamine to enhance material hydrophilicity. Remaining amino and double bond functional groups facilitated stepwise immobilization of trypsin and Glu-C. Digestion times for bovine serum albumin (BSA) and bovine hemoglobin (BHb) on the dual-IMER were significantly reduced compared to solution-based digestion (1 min vs. 36 h), resulting in improved sequence coverage (91.30% vs. 82.7% for BSA; 90.24% vs. 89.20% for BHb). Additionally, the dual-IMER demonstrated excellent durability, retaining 96.08% relative activity after 29 reuse cycles. Enhanced protein digestion efficiency can be attributed to several factors: (1) KIT-6’s large specific surface area, enabling higher enzyme loading capacity; (2) Its three-dimensional network pore structure, facilitating faster mass transfer and substance diffusion; (3) Orthogonality of trypsin and Glu-C enzyme cleavage sites; (4) The spatial effect introduced by the chain structure of PEI and glutaraldehyde’s spacing arm, reducing spatial hindrance and enhancing enzyme–substrate interactions; (5) Mild and stable enzyme immobilization. The KIT-6-based dual-IMER offers a promising technical tool for protein digestion, while the PDA/PEI/AM-KIT-6 platform holds potential for immobilizing other proteins or active substances.
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Biomass Conversion and Biorefinery
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2025
·
IF 4.1
Cui Yong,Wang Wenliang,Xu Xiaolu,Miao Hui,Fu Yishuai
In order to increase the yield of high-value components in bio-oil from waste wood-based panels (WWPs), a composite catalyst of SiC@HZSM-5 with both excellent capacities of microwave absorption and catalytic conversion was successfully prepared and applied to the microwave-assisted pyrolysis (MAP) with microwave power of 1000 W (2.45 GHz). The effects of different Si/Al ratios of HZSM-5 on the characterization of catalysts and selective pyrolysis products also have been investigated. The results showed that the obtained SiC@HZSM-5 catalyst exhibited a stable composite structure, favorable dielectric loss characteristics, and high thermal conductivity. Compared with the non-catalytic experiment, SiC@HZSM-5 catalysts significantly enhanced the relative content of nitrogen-containing compounds and reduced the concentration of oxygen-containing compounds in bio-oil, though there was one slight negative impact on bio-oil yield. Importantly, with the increase in Si/Al ratio from 30 to 90, the production of nitrogen-containing compounds was found to decrease from 37.11 to 23.14%, due to the reduction of acidic sites in catalysts. It was noteworthy that during the pyrolysis of WWPs by microwave heating, the catalyst with the Si/Al ratio of 30 significantly promoted the interactions between lignocellulosic components and adhesives and improved the selectivity of amide-N compounds (14.06%, acetamide being the main products) and heterocyclic-N compounds (23.05%, pyrroles, pyridines, and pyrazines constituted the major composition). This work could offer a promising technical reference to thermally converted WWPs to high value-added chemicals by microwave-assisted catalytic pyrolysis (MACP).
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JOURNAL OF THERMAL ANALYSIS AND CALORIMETRY
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2024
·Vol. 149
·期 24
·14715-14725
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IF 3
Chen Peng,Chen Liping,Zhou Juan,Wu Wenqian,Guo Zichao,Chen Wanghua
Thermal analysis and calorimetry techniques with sealed sample cells are frequently employed to investigate the thermal stability of hazardous materials. However, the influence of sample mass on thermal decomposition is often ignored. To investigate the effect of sample mass on autocatalytic decomposition substances under sealed condition, 2,4-DNT was chosen for test and analysis. The techniques including differential scanning calorimetry (DSC), rapid screening calorimetry(RSC), high-performance liquid chromatography-mass spectrometry (HPLC–MS), and gas chromatography-mass spectrometry (GC–MS) were utilized for in-depth research on the decomposition of 2,4-DNT. The results revealed that an increase in sample mass led to an significant elevation in gaseous products such as CO 2 and CO with a negative heat of formation (ΔH f ). Then the system pressure and decomposition heat of 2,4-DNT increase significantly, thereby enhancing the thermal decomposition process. After that, it has been determined that o–nitrotoluene, m–nitroaniline, and o–nitrobenzoic acid can catalyze the decomposition of 2,4-DNT. Finally, it is advisable to increase the sample mass as much as possible when testing and analyzing such nitro compounds which would help ensure that the predicted results are closer to the actual conditions of production, transport, storage.
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Nature Communications
·
2025
·Vol. 16
·期 1
·1-15
·
IF 15.7
Luan Xiaoxi,Tian Yu,Wu Fengxia,Cheng Lu,Tang Minghua,Lv Xiali,Wei Haili,Wang Xiaodan,Li Fenghua,Xu Guobao,Niu Wenxin
Helicoid metal nanoparticles with intrinsic chirality have unveiled tailorable properties and unlocked many chirality-related applications across various fields. Nevertheless, the existing strategies for enantioselective synthesis of helicoid metal nanoparticles have been predominantly limited to gold. Here, we demonstrate a robust and versatile strategy for the enantioselective synthesis of helicoid nanoparticles beyond gold, leveraging chiral nanoconfinement provided by chiral SiO 2 or nanoshells. The chiral nanoconfinement strategy enables the decoupling of ligand-directed crystal growth from chiral induction, allowing for the independent tuning of these two critical aspects. As a result, this approach can not only facilitate the replication of chiral shapes from the chiral nanoshells but also allow the generation of alternative chiral shapes. By employing this approach, we demonstrate the enantioselective synthesis of helicoid Pt, Au@Pt, Au@Pd, Au@Ag, and Au@Cu nanoparticles. The chiroplasmonic properties of Pt- and Pd-based chiral nanoparticles have been discovered, and the inversion of chiroplasmonic properties of Ag-based chiral nanoparticles via facet control has been documented and theoretically explained. The chiral nanoconfinement strategy enriches the toolbox for creating chiral nanoparticles and supports their exploration in diverse applications.
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Nature Communications
·
2024
·Vol. 15
·期 1
·1-10
·
IF 14.7
Wei Lei,Hai Xinyue,Xu Tongtong,Wang Zidi,Jiang Wentao,Jiang Shan,Wang Qisheng,Zhang Yue-Biao,Zhao Yingbo
Installing different chemical entities onto crystalline frameworks with well-defined spatial distributions represents a viable approach to achieve ordered and complex synthetic materials. Herein, a covalent organic framework (COF-305) is constructed from tetrakis(4-aminophenyl)methane and 2,3-dimethoxyterephthalaldehyde, which has the largest unit cell and asymmetric unit among known COFs. The ordered complexity of COF-305 is embodied by nine different stereoisomers of its constituents showing specific sequences on topologically equivalent sites, which can be attributed to its building blocks deviating from their intrinsically preferred simple packing geometries in their molecular crystals to adapt to the framework formation. The insight provided by COF-305 supplements the principle of covalent reticular design from the perspective of non-covalent interactions and opens opportunities for pursuing complex chemical sequences in molecular frameworks.
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Nature Communications
·
2026
·
IF 18.1
Zhu Yue,Liu Haoyang,Zhang Yu,Wang Jingjing,Mao Yanjin,Zhao Ruoyu,Sun Yuzhi,Zhang Yanan,Bao Xueyang,Chen Jingyu,Zheng Mingzhu,Chen Jialin,Ouyang Hongwei,Zhang Wei
Rapid and functional skin wound healing remains a major clinical challenge, while current biomaterial-based therapies largely serve as passive scaffolds or delivery vehicles. Whether biomaterials possess intrinsic bioactivities that instruct endogenous regenerative programs remains unclear. Here, independent of bulk structural/mechanical cues or exogenous biomolecules, we show that silk fibroin (SF), a clinically approved biomaterial, intrinsically programs keratinocytes into a Ccl27⁺ migratory-paracrine pro-regenerative state that coordinates epidermal-dermal crosstalk to drive regenerative wound healing. Integrated scRNA-seq, proteomics, and genetic perturbation reveal that SF engages keratinocyte ITGA2 to activate PI3K-Akt signaling and relieve P53-mediated Ccl27 repression. Elevated Ccl27 enhances keratinocyte migration and re-epithelialization, while its secretion from SF-activated keratinocytes promotes fibroblast proliferation and dermal remodeling, collectively enabling wound closure and appendage reconstruction. Keratinocyte-specific Ccl27 knockdown largely abrogates SF-mediated regeneration in vivo. These findings uncover the intrinsic bioactivity of SF and establish biomaterials as instructive cues rather than passive scaffolds for functional tissue repair.
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JOURNAL OF SOLID STATE ELECTROCHEMISTRY
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2024
·
IF 2.6
Ravichandran Balamurali,Narayanan Naresh,Ravichandran Sabarinathan,Liu Huiyuan,Zhang Weiqi,Bhuvanendran Narayanamoorthy,Su Huaneng
In this study, PtCo alloy nanoparticles (NPs) were successfully synthesized and deposited on nitrogen-rich carbon nanosheets derived from Polypyrrole-g-C 3 N 4 using a chemical reduction method. This electrocatalyst not only offers enhanced catalytic efficiency but also significantly improves the stability for the oxygen reduction reaction (ORR) in in acidic medium. In terms of electrocatalytic performance, the PtCo/CN@PPY-g-C 3 N 4 catalyst demonstrated a mass activity of 0.378 mA µg Pt −1 at 0.85 V, 0.131 mA µg Pt −1 at 0.9 V and a specific activity of 2.900 mA cm Pt −2 at 0.85 V, 1.004 mA cm Pt −2 at 0.9 V which are respectively 2.3, 2.8 and 10, 12 times higher than those of a commercial 20% Pt/C catalyst (0.166 mA µg Pt −1 at 0.85 V, 0.046 mA µg Pt −1 at 0.9 V and 0.285 mA cm Pt −2 at 0.85 V, 0.079 mA cm Pt −2 at 0.9 V). This indicates superior catalytic activity. Furthermore, after 5000 cycles, the PtCo/CN@PPY-g-C 3 N 4 retained approximately 77% at 0.85 V and 83% at 0.9 V of its initial mass activity, with only a 14 mV decrease in the half-wave potential, whereas commercial 20% Pt/C catalyst retained only 40% at0.85 V and 30% at 0.9 V of its initial mass activity. These enhancements can be attributed to the synergistic effects and strong interactions between the Pt–Co alloy nanoparticles and the carbon nitride support. The findings of this study underscore the potential of PtCo/CN@PPY-g-C 3 N 4 as a viable and efficient alternative to traditional catalysts in electrochemical applications.
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JOURNAL OF THERMAL ANALYSIS AND CALORIMETRY
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2024
·Vol. 149
·期 12
·5985-5994
·
IF 3
Liao Xueqin,Du Longjin,Sun Mengxia,Liu Hui,Liu Jianzhong
Aluminum has been long and widely used in propellants, thermite, and pyrotechnics. However, the commonly used micron aluminum has the problems of slow burning rate and incomplete release of chemical energy in the combustion process. Therefore, finding ways to promote the ignition and combustion of aluminum is essential to enhance the performance of aluminum-based energetic materials. Herein, environmentally friendly tannic acid (TA) and tea polyphenols (TP) were used to surface modify aluminum to improve its combustion performance, respectively. The results showed that TA and TP could be coated on the outer surface of aluminum particles effectively by solvent evaporation induced self-assembly method. Heat was released during the thermal decomposition of the composite particles, which was clearly beneficial in promoting aluminum ignition. Ignition and combustion tests showed that after modification with TA and TP, the samples showed shorter ignition delay, increased combustion time, increased combustion intensity, and reduced agglomeration compared to pure aluminum. This revealed that the modified samples possessed better ignition and combustion properties. The X-ray diffraction patterns of the condensed combustion products showed that the products of pure aluminum possessed more active aluminum and aluminum nitride, while the samples modified with TA and TP had more γ-Al 2 O 3 and α-Al 2 O 3 , suggesting that TA and TP could effectively enhance the combustion efficiency of aluminum. In addition, TP showed better modification than TA, which may be related to the higher heat release during thermal decomposition of TP. Although TA and TP can improve the ignition and combustion performance of aluminum to some extent, the enhancement effect is limited. Considering that TA and TP possess good adhesion, perhaps they can play a better role in promoting as intermediate layers in the future. It is hoped that this paper can provide a new idea for the surface modification of aluminum.
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Journal of Food Measurement and Characterization
·
2025
·
IF 3.3
Wu Yi,Hu Hui,Yang Guangzhen,Chen Yifan,Zhong Xiaoyu,Li Shanshan,Pan Ming,Li Yuanbin,Zhang Jiaheng
Natural deep eutectic solvents (NaDESs) with ultrasound-assisted (UAE) method was used to improve the extraction efficiency of polysaccharide from black truffles, which can overcome the limitations of conventional methods. It demonstrated that the extraction efficiency of polysaccharide by six NaDESs with UAE were better than those of conventional solvents. Among the NaDESs, betaine-citric acid (BetCi) NaDES showed the best performance, achieving a remarkable extraction yield of 9.38%, outperforming water, ethanol, and 1,3-butanediol by 0.4, 11.5 and 11.0 times, respectively. Moreover, the polysaccharide yield was further optimized by response surface method, based on three influence factors (NaDES concentration, liquid-to-solid, and ultrasonic time). Scanning electron microscopy images showed that compared to the traditional extraction methods, the plate-like structure fractured into smaller pieces of black truffles treated by BetCi NaDES with UAE, indicating deeper interactions between NaDES and black truffles. Furthermore, the skin penetration as well as antioxidant and anti-wrinkle properties of the black truffle polysaccharide extract were evaluated, revealing promising bioactivity. Overall, this study represents a comprehensive exploration of novel extraction methods, highlighting efficient polysaccharide extraction and its potential applications.
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ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
·
2024
·
IF 0
Hammad Eman N.,Eltaweil Abdelazeem S.,Abouelenein Saeyda A.,El-Subruiti Gehan
Water contamination by heavy metals, especially chromium (VI), poses a critical environmental issue due to its carcinogenic nature and persistence in the environment. Addressing this, the current study develops an efficient adsorbent, CPBr-MIL-88A@AmGO, which utilizes the synergistic capabilities of Cetylpyridinium bromide–modified MIL-88A and amine-functionalized graphene oxide to enhance Cr(VI) removal from aqueous solutions. The obtained results indicate that CPBr-MIL-88A@AmGO achieves its highest removal efficacy at pH 2, where the interaction of CPBr and AmGO’s positively charged centers significantly contributes to the adsorption processes. According to the Langmuir isotherm model, the composite’s adsorption capacity reached a maximum of 306.75 mg/g. The adsorption kinetics adhered to a pseudo-second-order model along with the endothermic nature of the process. Although the presence of SO 4 2- ions significantly reduces adsorption capacity, other interfering ions including Na + , K + , Ca 2+ , Cl - , and NO 3 - only slightly affect it. Remarkably, the composite maintains high removal efficiency, over 82%, even after 7 recycling tests, underscoring its potential for practical applications in water treatment systems. The proposed mechanism involves the contribution of electrostatic attractions, ion exchange, complexation, and the reduction of Cr(VI) to Cr(III) in the removal process. This study not only offers a potent solution for Cr(VI) remediation but also contributes to sustainable water resource management.
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npj Clean Water
·
2024
·Vol. 7
·期 1
·1-10
·
IF 10.4
Shu Dawu,Zhang Xinqi,Han Bo,Li Wanxin,Wang Bingxin,Xu Chengshu
Cobalt-based catalysts were synthesized using the molten metal salt method and applied for the degradation of reactive dyeing wastewater. The results demonstrated a degradation of 97.1% for the C.I. Reactive Red 195 solution under the following conditions: 1.0 g/L of Co@MXene, 3 g/L of peroxymonosulfate (PMS), treated at 25 o C for 36 min with initial pH of 7. After adding 20 g/L of NaCl, the dye degradation rate increased to 5.57 times compared to the original rate 0.0894 min −1 , but the difference in final degradation was not significant. The enhanced degradation was attributed to the combined action of hydroxyl radicals (•OH), sulfate radicals (SO 4 •− ), and singlet oxygen ( 1 O 2 ). Notably, the Co@MXene catalyst maintained a high dye degradation percentage of 93.5% even after being recycled ten times. The treated dye residue can be recycled for dyeing cotton fabrics with reactive dyes. This study achieves rapid treatment of dye wastewater with wide applicability and provides valuable insights into dye wastewater treatment and environmental remediation.
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Nature Communications
·
2024
·Vol. 15
·期 1
·1-11
·
IF 14.7
Pei An,Wang Peng,Zhang Shiyi,Zhang Qinghua,Jiang Xiaoyi,Chen Zhaoxi,Zhou Weiwei,Qin Qizhen,Liu Renfeng,Du Ruian,Li Zhengjian,Qiu Yongcai,Yan Keyou,Gu Lin,Ye Jinyu,Waterhouse Geoffrey I. N.,Huang Wei-Hsiang,Chen Chi-Liang,Zhao Yun,Chen Guangxu
Challenges in direct catalytic oxidation of biomass-derived aldehyde and alcohol into acid with high activity and selectivity hinder the widespread biomass application. Herein, we demonstrate that a Pd/Ni(OH) 2 catalyst with abundant Ni 2+ -O-Pd interfaces allows electrooxidation of 5-hydroxymethylfurfural to 2, 5-furandicarboxylic acid with a selectivity near 100 % and 2, 5-furandicarboxylic acid yield of 97.3% at 0.6 volts (versus a reversible hydrogen electrode) in 1 M KOH electrolyte under ambient conditions. The rate-determining step of the intermediate oxidation of 5-hydroxymethyl-2-furancarboxylic acid is promoted by the increased OH species and low C–H activation energy barrier at Ni 2+ -O-Pd interfaces. Further, the Ni 2+ -O-Pd interfaces prevent the agglomeration of Pd nanoparticles during the reaction, greatly improving the stability of the catalyst. In this work, Pd/Ni(OH) 2 catalyst can achieve 100% 5-hydroxymethylfurfural conversion and >90% 2, 5-furandicarboxylic acid selectivity in a flow-cell and work stably over 200 h under a fixed cell voltage of 0.85 V.
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