Scientific Reports
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2018
·Vol. 8
·期 1
·1-11
·
IF 4.122
Li Huihui,Zhao Hongting,Hao Shuangying,Shang Longcheng,Wu Jing,Song Chuanhui,Meyron-Holtz Esther G.,Qiao Tong,Li Kuanyu
Iron is essential for growth and proliferation of mammalian cells. The maintenance of cellular iron homeostasis is regulated by iron regulatory proteins (IRPs) through binding to the cognate iron-responsive elements in target mRNAs and thereby regulating the expression of target genes. Irp1 or Irp2 -null mutation is known to reduce the cellular iron level by decreasing transferrin receptor 1 and increasing ferritin. Here, we report that Irp1 or Irp2 -null mutation also causes downregulation of frataxin and IscU, two of the core components in the iron-sulfur cluster biogenesis machinery. Interestingly, while the activities of some of iron-sulfur cluster-containing enzymes including mitochondrial aconitase and cytosolic xanthine oxidase were not affected by the mutations, the activities of respiratory chain complexes were drastically diminished resulting in mitochondrial dysfunction. Overexpression of human ISCU and frataxin in Irp1 or Irp2 -null cells was able to rescue the defects in iron-sulfur cluster biogenesis and mitochondrial quality. Our results strongly suggest that iron regulatory proteins regulate the part of iron sulfur cluster biogenesis tailored specifically for mitochondrial electron transport chain complexes.
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高影响因子
MICROCHIMICA ACTA
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2018
·Vol. 185
·期 10
·1-9
·
IF 5.705
Jiao Zhe,Li Jingwen,Mo Liangji,Liang Jinming,Fan Hongbo
A molecularly imprinted polymer (MIP) was fabricated for selective recognition of the highly persistent pollutant perfluorooctane sulfonate (PFOS). The MIP was prepared from chitosan and doped with fluorescent carbon quantum dots (CQDs). It was characterized by fluorescence spectrophotometry, scanning electron microscopy, and Fourier transform infrared spectroscopy. The fluorescence of the CQDs, best measured at excitation/emission wavelengths of 350/460 nm, is enhanced by PFOS, and the effect is much stronger for the MIP than for the nonimprinted polymer (NIP). The imprinting factor is 2.75. The method has good specificity over sodium dodecyl sulfate (SDS), perfluorooctanoic acid (PFOA), sodium dodecyl sulfonate (SDS’), sodium dodecyl benzene sulfonate (SDBS), perfluorooctanesulfonyl fluoride (POSF), perfluorobutane sulfonate (PFBS) and 1-octanesulfonic acid sodium (OSA). Fluorescence increases linearly in the 20–200 pg·L −1 POSF concentration range in aqueous solution. The method was applied to the determination of PFOS in spiked serum and urine samples. The limits of detection are 66 and 85 pg·L −1 for serum and urine samples respectively. The recoveries ranged from to 81–98%, with relative standard deviations in the range of 1.8–8.2%. Compared with LC-MS/MS, this assay is more convenient since the material can be prepared flexibly and the method can be applied on-site.
Schematic of the fabrication of a molecularly imprinted chitosan hydrogel doped with CQDs for selective fluorometric determination of PFOS. a. The photo of chitosan hydrogel. b, c, d, e represents the hydrogel observed under UV lamp. b’, c’, d’, e’ represents the inner structure of hydrogel bead.
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高影响因子
JOURNAL OF MATERIALS SCIENCE
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2018
·Vol. 53
·期 21
·14989-14997
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IF 2.993
Jia Qin,Geng Zhi-Qin,Liu Yang,Wang Wen,Han Cai-Qin,Yang Guo-Hai,Li Haitao,Qu Lu-Lu
A disposable solid-phase extraction (SPE) membrane was developed for rapid removal and sensitive surface-enhanced Raman scattering (SERS) detection of antibiotics in water samples. The membrane was fabricated on commercially available SPE column by filtration of the activated carbon modified with silver nanoparticles (Ag NPs/AC). The prepared SPE membrane exhibited outstanding preconcentration ability due to the high adsorption properties of AC, and excellent ability to enhance Raman signal resulting from “hot spots” between the embedded Ag NPs, improving the sensitivity of SERS detection. A detection limit (LOD) of 5.0 × 10 −11 and 1.6 × 10 −10 M was achieved for rhodamine 6G and p -aminothiophenol. In addition, the membrane exhibited high reproducibility with spot-to-spot variation in SERS spectral intensity less than 15%. Based on the membrane, the qualitative and quantitative analysis of the antibiotics in aqueous solution was accomplished with the LOD at nM level, demonstrating the feasibility of the disposable SPE membrane for in situ rapid preconcentration and detection.
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高影响因子
Science China-Chemistry
·
2018
·Vol. 61
·期 9
·1159-1166
·
IF 4.448
Chen Miao,Zhang Wei-Guo,Li Jia-Wei,Hong Chun-Yan,Zhang Wen-Jian,You Ye-Zi
pH- and reductive-responsive prodrug nanoparticles are constructed via a highly efficient strategy, polymerization-induced selfassembly (PISA). First, reversible addition-fragmentation chain transfer (RAFT) polymerization of 2-(diisopropylamino) ethyl methacrylate (DIPEMA) and camptothecin prodrug monomer (CPTM) using biocompatible poly( N -(2-hydroxypropyl) methacrylamide) (PHPMA-CPDB) as the macro RAFT agent is carried out, forming prodrug diblock copolymer PHPMA-P (DIPEMA- co -CPTM). Then, simultaneous fulfillment of polymerization, self-assembly, and drug encapsulation are achieved via RAFT dispersion polymerization of benzyl methacrylate (BzMA) using the PHPMA-P(DIPEMA- co -CPTM) as the macro RAFT agent. The prodrug nanoparticles have three layers, the biocompatible shell (PHPMA), the drug-conjugated middle layer (P(DIPEMA- co -CPTM)) and the PBzMA core, and relatively high concentration (250 mg/g). The prodrug nanoparticles can respond to two stimuli (reductive and acidic conditions). Due to reductive microenvironment of cytosol, the cleavage of the conjugated camptothecin (CPT) within the prodrug nanoparticles could be effectively triggered. pH-Induced hydrophobic/ hydrophilic transition of the PDIPEMA chains results in faster diffusion of GSH into the CPTM units, thus accelerated release of CPT is observed in mild acidic and reductive conditions. Cell viability assays show that the prodrug nanoparticles exhibit well performance of intracellular drug delivery and good anticancer activity.
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高影响因子
COLLOID AND POLYMER SCIENCE
·
2018
·Vol. 296
·期 8
·1365-1372
·
IF 1.967
Zhang Rongli,Jin Zhenzhen,Zhang Cuige,Yang Renchun,Sun Mingyue,Wong Ching-Ping
A kind of electroactive nanoparticles (NPs) was prepared in solution by self-assembling amphiphilic 3-thiophenecarboxylic acid-modified starch (TPCA-St), which was synthesized through esterification reaction. By casting TPCA-St NPs on the surface of gold electrode and subsequent electropolymerization of the thiophene moieties in NP film, the electroconductive TPCA-St NP-based film was formed. After horseradish peroxidase and Nafion were sequentially cast on the film surface, a biosensor was successfully prepared. The prepared biosensor showed high sensitivity for H 2 O 2 detection. The linear range from 1 × 10 −10 to 1 × 10 −5 mol L −1 with a detection limit of 3 × 10 −11 mol L −1 was obtained for H 2 O 2 sensing. The biosensor also showed good repeatability and stability, and it has been successfully used to sense H 2 O 2 in commercial disinfector with satisfactory results.
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ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
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2018
·Vol. 26
·期 33
·33847-33855
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IF 2.8
Wang He-xuan,Zhu Li-nan,Guo Fu-qiao
Atrazine, one of the most widespread herbicides in the world, is considered as an environmental estrogen and has potential carcinogenicity. In this study, atrazine was degraded on boron-fluorine co-doped TiO 2 nanotube arrays (B, F-TiO 2 NTAs), which had similar morphology with the pristine TiO 2 NTAs. The structure and morphology of TiO 2 nanotube samples were characterized by scanning electron microscopy (SEM), X-ray diffraction (XRD), and UV-visible diffuse reflectance spectroscopy (DRS). It showed that the decoration of fluorine and boron made both the absorption in the visible region enhanced and the band edge absorption shifted. The efficiency of atrazine degradation by B, F-TiO 2 NTAs through photoelectrocatalysis was investigated by current, solution pH, and electrolyte concentration, respectively. The atrazine removal rate reached 76% through photoelectrocatalytic reaction by B, F-TiO 2 NTAs, which was 46% higher than that under the photocatalysis process. Moreover, the maximum degradation rate was achieved at pH of 6 in 0.01 M of Na 2 SO 4 electrolyte solution under a current of 0.02 A and visible light for 2 h in the presence of B, F-TiO 2 NTAs. These results showed that B, F-TiO 2 NTAs exhibit remarkable photoelectrocatalytic activity in degradation of atrazine.
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高影响因子
MICROCHIMICA ACTA
·
2018
·Vol. 185
·期 7
·1-9
·
IF 5.705
Li Xinpei,Sun Ying,Yuan Liming,Liang Li,Jiang Yanxiao,Piao Huilan,Song Daqian,Yu Aimin,Wang Xinghua
This work describes a nanohybrid material consisting of gold nanoparticles (AuNPs) and nanosheets of layered double hydroxides (NLDHs) of Mg(II) and Al(III). Mono-disperse AuNPs were immobilized on the surface of the LDHs via Au-O bonding. The nanohybrid sorbent was packed in an organic filter along with a syringe and applied to the microextraction of triazine herbicides with the help of an injection pump. The collected hexane eluate was concentrated and directly injected into a HPLC column for quantification. The effects of the amount of Au/LDH nanohydrobrids, type, flow rate, volume of washing and eluting solvent were optimized. The method was validated by detecting four triazine herbicides (prometryn, atrazine, terbumeton and secbumeton) in spiked maize. The limits of detection range between 35 and 108 pg g −1 . The relative standard deviations range from 1.0–6.9% for repeatability and 4.6–7.8% for reproducibility (for n = 5).
Schematic presentation of a nanohybrid material consisting of gold nanoparticles and nanosheets of layered double hydroxides of Mg(II) and Al(III) (Au/LDH) for use as an adsorbent for microextraction in a packed syringe. Organic filter heads were used as the container of Au/LDHs nanohybrids, and were connected with the syringes installed on the injection pump for the semi-automatic microextraction and preconcentration of triazines in maize.
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高影响因子
MACROMOLECULAR RESEARCH
·
2018
·Vol. 26
·期 9
·800-807
·
IF 1.767
Wang Chao,Li Zhenhuan,Cao Lei,Cheng Bowen
Polyphenylene sulfide (PPS) membranes were modified by chloromethylation and quaternary amination reaction to prepare a hydrophilic PPS microporous membrane. The structures of the modified PPS membranes were characterized by the use of Fourier transform infrared spectroscopy, X-ray photoelectron spectroscopy, and scanning electron microscopy. The results showed that the modified membranes maintain the same microporous structures as the PPS membrane. The hydrophilicity of the modified membranes was significantly improved compared to that of the PPS membranes, and the water flux of N-methylimidazole modified membranes, triethylamine modified membranes, and both mixture modified membranes achieved 110.475, 168.94, 150.59 L·m −2 ·h −1 , respectively. The quaternary amination of the PPS membranes made much less bovine serum albumin (BSA) pollution accumulation on the top surface of the modified membranes, and there was a significant improvement, to 94%, in the BSA rejection rate. Most importantly, the antibacterial activity of the modified membranes was effectively improved, and the bactericidal rate was elevated to above 80% in comparison with pure PPS membranes, which was attributed to the introduction of quaternary ammonium salts and imidazolium salts in out-surface of the membranes.
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高影响因子
Frontiers of Environmental Science & Engineering
·
2018
·Vol. 12
·期 3
·1-10
·
IF 1.961
Wu Zhichao,Zhang Chang,Peng Kaiming,Wang Qiaoying,Wang Zhiwei
Membrane technology for oil/water separation has received increasing attention in recent years. In this study, the hydrophilic/underwater superoleophobic membrane with enhanced water permeability and antifouling ability were fabricated by synergistically assembling graphene oxide (GO) nanosheets and titanium dioxide (TiO 2 ) nanotubes for oil/water separation. GO/TiO 2 membrane exhibits hydrophilic and underwater superoleophobic properties with water contact angle of 62° and under water oil contact angle of 162.8°. GO/TiO 2 membrane shows greater water permeability with the water flux up to 531 L/ (m2$h$bar), which was more than 5 times that of the pristine GO membrane. Moreover, GO/TiO 2 membrane had excellent oil/water separation efficiency and anti-oil-fouling capability, as oil residual in filtrate after separation was below 5 mg/L and flux recovery ratios were over 80%.The results indicate that the intercalation of TiO 2 nanotubes into adjacent GO nanosheets enlarged the channel structure and modified surface topography of the obtained GO/TiO 2 membranes, which improved the hydrophilicity, permeability and anti-oil-fouling ability of the membranes, enlightening the great prospects of GO/TiO 2 membrane in oil-water treatment.
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高影响因子
JOURNAL OF SOLUTION CHEMISTRY
·
2018
·Vol. 47
·期 4
·705-714
·
IF 1.401
Li Qiang,Liu Bijun,Mu Wanjun,Yu Qianhong,Tian Yin,Liu Guoping,Yang Yuchuan,Li Xingliang,Luo Shunzhong
Glufosinate, an analogue of glutamic acid (also known as phosphinothricin), is an important herbicide. The protonation constants of glufosinate were determined by means of potentiometric titrations at variable temperatures with ionic strength 0.5 mol·L −1 (NaCl solution). The heat effects of the protonation reactions of glufosinate were measured by direct calorimetry. NMR spectroscopies have demonstrated that the first protonation site occurs on the nitrogen atom in the amino group, followed by one of the oxygen atoms in the phosphono group, and finally the carboxyl oxygen atom. This trend is in good agreement with the enthalpy of protonation and crystal structure results. These data will help to predict the speciation of glufosinate in physiological systems.
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JOURNAL OF THERMAL ANALYSIS AND CALORIMETRY
·
2018
·Vol. 135
·期 5
·2869-2877
·
IF 2.209
Wang Dongqing,Sui Jian,Qi Di,Deng Shuping,Wei Yongteng,Wang Xin,Lan XiaoZheng
Confinement effect of nanopores on phase transition of docosane ( n -C 22 H 46 , C 22 ) is studied using differential scanning calorimetry (DSC) and temperature-dependent powdered X-ray diffractions (XRD). The bulk C 22 has a stable rotator phase R I and R II . In the pores of CPGs, SBA-15, C-SBA-15, KIT-6, and MCM-41, the transition temperatures of solid–solid ( T s ) and solid–liquid ( T m ) transitions of C 22 are depressed. The depression of the transition points (Δ T s , Δ T m ) has a linear relation with the reverse diameter. However, the Δ T m values seemingly show weak relation with the polarity of the pore walls but to vary with the pore dimensionality. According to XRD analysis, C 22 confined in CPG (11.5 nm) and SBA-15 (14.4 nm) present low temperature phase and rotator phase as the bulk, but the lamellar packing of molecules is heavily disturbed in the small pores.
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高影响因子
JOURNAL OF ELECTRONIC MATERIALS
·
2018
·Vol. 47
·期 7
·4085-4089
·
IF 1.566
Shao Li-Huan,Shen Si-Yun,Zheng Hui,Zheng Peng,Wu Qiong,Zheng Liang
Compact hexagonal barium ferrite (BaFe 12 O 19 , BaM) ceramics with excellent magnetic properties have been prepared from powder with the optimal grain size. The dependence of the microstructure and magnetic properties of the ceramics on powder grain size was studied in detail. Single-phase hexagonal barium ferrite powder with grain size of 177 nm, 256 nm, 327 nm, and 454 nm was obtained by calcination under different conditions. Scanning electron microscopy revealed that 327-nm powder was beneficial for obtaining homogeneous grain size and compact ceramic. In addition, magnetic hysteresis loops and complex permeability spectra demonstrated that the highest saturation magnetization (67.2 emu/g) and real part of the permeability (1.11) at 1 GHz were also obtained using powder with grain size of 327 nm. This relationship between the powder grain size and the properties of the resulting BaM ceramic could be significant for development of microwave devices.
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