International Journal of Electrochemical Science
·
2023
·
IF 1.5
Kangquan Qiao,Ying Zeng
The inhibition effect of Diantipyrylmethane (DAM) and its synergistic effect with potassium thiocyanate (KSCN) on mild steel corrosion in 1 M HCl solution were investigated by electrochemical methods, gravimetric analysis and scanning electron microscopy (SEM). The experiment results show that the inhibition efficiency increases with the concentration of DAM alone and increases further with the presence of 2 mM KSCN. The synergism parameters demonstrate that DAM cation has a significant synergistic effect with thiocyanate anion, which is agree with the corresponding thermodynamic and kinetic parameters. The results of quantum chemical calculation confirm that the dication structure of DAM molecule in acid medium plays a vital role during corrosion inhibition of mild steel.
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International Journal of Electrochemical Science
·
2023
·
IF 1.5
Yanyan Xu,Wenwen Xiao,Pengli Ge,Wenguang Zeng,Qingshan Liu,Zhengyuan Gao,Yongbo Yan
This work aimed to develop a high-efficiency and reliable alternative for conventional and toxic corrosion inhibitor. A novel tetrazole derivative (1-Phenyltetrazole-5-thiol, PTT) that possessed excellent anti-corrosion capacity was exploited for the first time. Based on the traditional electrochemical investigation, morphological observation, and computer modeling, inhibition ability and adsorption type of PTT for X70 steel in 0.5 M H 2 SO 4 were varied. The electrochemical results showed that PTT was a mixed organic inhibitor, which could retard charge transfer impedance, and slowed down the corrosion rate of steel. Specifically, the highest inhibition efficiency was 95.1% when the added PTT dose was 2 mM. The adsorption model showed that the PTT film was formed via powerful physicochemical adsorption. As shown in theoretical calculations, the lower Δ E , higher μ values and E binding value of PTT corresponded to supreme adsorption performance, which was also a manifestation of the PTT high-efficiency corrosion inhibition.
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JOURNAL OF COLLOID AND INTERFACE SCIENCE
·
2023
·
IF 9.9
Kunjie Wang,Yan Jing,Shuang Gao,Xianrong Liu,Bingxin Liu,Yongcheng Li,Peng Zhang,Benhua Xu
Implanting the octahedral phase (1 T) into the hexagonal phase (2H) of the molybdenum disulfide (MoS 2 ) matrix is considered one of the effective methods to enhance hydrogen evolution reaction (HER) performances of MoS 2 . In this paper, hybrid 1 T/2H MoS 2 nanosheets array was successfully grown on conductive carbon cloth (1 T/2H MoS 2 /CC) via facile hydrothermal method and the 1 T phase content in 1 T/2H MoS 2 is regulated to gradually increase from 0 % to 80 %. 1 T/2H MoS 2 /CC with 75 % 1 T phase content exhibits optimal HER performances. The DFT calculation results show that S atoms in 1 T/2H MoS 2 interface exhibit the lowest hydrogen adsorption Gibbs free energies (ΔG H* ) compared with other sites. The improvement of HER performances are primarily attributed to activating the in-plane interface regions of the hybrid 1 T/2H MoS 2 nanosheets. Furthermore, the relationship between 1 T MoS 2 content in 1 T/2H MoS 2 and catalytic activity was simulated by a mathematical model, which shows that the catalytic activity presents a trend of increasing and then decreasing with the increase of 1 T phase content.
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TOXICOLOGY AND APPLIED PHARMACOLOGY
·
2023
·
IF 3.8
Hancheng Huang,Shuchun Wei,Xiaohan Wu,Mengke Zhang,Benhong Zhou,Debin Huang,Weiguo Dong
The pathophysiological mechanism of hepatic fibrosis (HF) is related to the excessive activation of the DNA repair enzyme poly ADP-ribose polymerase-1 (PARP-1). The drugs, targeting PARP-1, are scarce. Therefore, the lead compound, moderately inhibiting PARP-1, with anti-HF properties should be identified. This study screened dihydrokaempferol (DHK) from herbs based on preliminary studies to intervene in a CCl 4 -induced liver injury and HF model in mice. In vitro , the expression levels of PARP-1-regulated related proteins and phosphorylation were examined. The binding pattern of DHK and PARP-1 was analyzed using molecular docking and molecular dynamics platforms. The results showed that DHK could significantly attenuate CCl 4 -induced liver injury and HF in mice. Moreover, it could also attenuate the toxic effects of CCl 4 on HepG2 and inhibit α-SMA and Collagen 1/3 synthesis of LX-2 cells in-vitro . Molecular docking revealed that DHK could competitively bind to the Glu-988 and His-862 residues of the upstream DNA repair enzyme PARP-1, moderately inhibiting its overactivation. This led to maintaining NAD + levels and energy metabolism in hepatocytes and inhibiting the activation of PARP-1-regulated downstream signaling pathways (TGF-β1, etc. ), related proteins (p-Smd2/3, etc. ), and inflammatory mediators while acting indirectly. Thus, DHK could attenuate CCl 4 -induced liver injury and HF in mice in a different mechanism from those of the existing reported flavonoids. It was associated with inhibiting the expression of downstream pathways and related cytokines by competitively binding to PARP-1. This study might provide a basis and direction for the design and exploration of anti-HF lead compounds.
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JOURNAL OF NANOBIOTECHNOLOGY
·
2023
·Vol. 21
·期 1
·1-15
·
IF 10.2
Su Runping,Gu Jingjing,Sun Juanjuan,Zang Jie,Zhao Yuge,Zhang Tingting,Chen Yingna,Chong Gaowei,Yin Weimin,Zheng Xiao,Liu Bingbing,Huang Li,Ruan Shuangrong,Dong Haiqing,Li Yan,Li Yongyong
Antigen self-assembly nanovaccines advance the minimalist design of therapeutic cancer vaccines, but the issue of inefficient cross-presentation has not yet been fully addressed. Herein, we report a unique approach by combining the concepts of “antigen multi-copy display” and “calcium carbonate (CaCO 3 ) biomineralization” to increase cross-presentation. Based on this strategy, we successfully construct sub-100 nm biomineralized antigen nanosponges (BANSs) with high CaCO 3 loading (38.13 wt%) and antigen density (61.87%). BANSs can be effectively uptaken by immature antigen-presenting cells (APCs) in the lymph node upon subcutaneous injection. Achieving efficient spatiotemporal coordination of antigen cross-presentation and immune effects, BANSs induce the production of CD4 + T helper cells and cytotoxic T lymphocytes, resulting in effective tumor growth inhibition. BANSs combined with anti-PD-1 antibodies synergistically enhance anti-tumor immunity and reverse the tumor immunosuppressive microenvironment. Overall, this CaCO 3 powder-mediated biomineralization of antigen nanosponges offer a robust and safe strategy for cancer immunotherapy.
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APPLIED CATALYSIS A-GENERAL
·
2022
·
IF 5.723
Yi-Tian Wang,Si-Ming Wu,Guo-Qiang Luo,Ge Tian,Bo-Jun Zeng,Li-Ying Wang,Shi-Tian Xiao,Jing-Xian Wu,Ao Wu,Kang-Jun Wu,Silvia Lenaerts,Xiao-Yu Yang
Dual active sites from acidic zeolite and Pd are not only capable of catalyzing multiple type of reactions, but could also generate unique functions owing to the synergy between metals and acidic sites. However, there are only a few reports on the investigation of the synergy of acid/Pd dual sites in TS-1. Herein, TS-1 confined Pd catalyst with mesoporous silica shell (Pd@TS-1 @meso-SiO 2 ) has been successfully synthesized and its synergy effect contributes to the enhanced conversion rate (19.2%) and selectivity (74.7%) on styrene oxidation . The interaction between Pd and TS-1 has been investigated by EPR and 1 H NMR techniques, the experimental measurements show an obvious change in the signal distribution of weakly acidic terminal hydroxyls and hydrogen-bonding silanols. The schematic illustration of selective styrene oxidation in the model of Pd@TS-1 @meso-SiO 2 is proposed to clarify the synergistic effect on styrene oxidation between TS-1 and Pd nanoparticles at an atomic-/nanoscale.
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JOURNAL OF BIOTECHNOLOGY
·
2019
·
IF 3.163
Xiao-Ling Tang,Nan-Nan Zhang,Guo-Yan Ye,Yu-Guo Zheng
( R )-3-amino-1-butanol is a key intermediate of Dolutegravir for the treatment of HIV/AIDS and its green and efficient biosynthesis has attracted a great deal of attention. Transaminases are currently used as promising biocatalyst for the synthesis of chiral amines. However, many transaminases have ( S )-specificity and ( R )-selective transaminases were less exploited and studied, making the production of ( R )-amines remain challenging. In this study, a novel transaminase from Actinobacteria sp. ( As -TA) was obtained and applied for the biosynthesis of ( R )-3-amino-1-butanol by transferring the amino group from isopropylamine to 4-hydroxy-2-butanone. After optimization of the reaction condition and using a substrate fed-batch strategy, the conversion of 100, 200, 300, 400 and 500 mM 4-hydroxy-2-butanone reached 100%, 94.9%, 86.1%, 76.1% and 70.9%, respectively. ( R )-3-amino-1-butanol with a maximum yield of 29.6 g/L and 99.9% e.e. value was obtained. This was the first time demonstrating the successful biosynthesis of ( R )-3-amino-1-butanol with transaminase as biocatalyst and the obtained As -TA enriched the enzyme pool of transaminase with ( R )-specificity.
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JOURNAL OF NUCLEAR MATERIALS
·
2018
·
IF 2.447
Xin Wang,Min Xue,Songbai Liu,Kuo Jiang
In this paper, (Gd 1− x Ce x ) 2 Zr 2 O 7+ x immobilized matrices with high cerium (Ce) amount-of-substance fractions (up to 90%) were prepared by sol-spray pyrolysis , and then sintered at 1600 °C in air. XRD results showed that all samples were in a mixture of pyrochlore and fluorite phase, even after sintering at 1600 °C. All the sintered bulks were dense, and the densities were independent of the additive content . The SEM results showed that all samples were chemically homogeneous. There was no segregation in the grain boundary. EDS quantification yielded a mean composition close to the stoichiometric chemical composition.
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ENVIRONMENTAL ENTOMOLOGY
·
2018
·Vol. 48
·期 1
·245-252
·
IF 1.661
Wang Huatang,Chen Huiling,Wang Zhengbing,Liu Jiali,Zhang Xingyan,Li Chaofeng,Zeng Xinnian
For insects, odorant-binding proteins (OBPs) play an essential role in binding and transporting semiochemicals through the sensillum lymph to olfactory receptor neurons within the antennal sensilla. In the present study, the full-length cDNA encoding a general odorant-binding protein 1 (DcitOBP1, accession number KY475564) was cloned from the antennae of Diaphorina citri using RACE-PCR, and qRT-PCR analysis revealed that the DcitOBP1 gene was expressed mainly in the antennae of D. citri. In molecular docking assay, the results showed that DcitOBP1 protein has better binding affinities to the 12 selected host-plant volatile compounds. Then, the recombinant DcitOBP1 protein was expressed in Escherichia coli. After removed His-Tag, the binding properties of purified DcitOBP1 protein to the selected host-plant volatile compounds were investigated in a fluorescence ligand-binding assay, similar, but more obviously binding properties of DcitOBP1 protein result were obtained, the dissociation constant (KD) value of DcitOBP1/1-NPN complex was 6.440 ± 0.521, and the DcitOBP1 protein showed high binding affinities (IC50 < 100 μM) to six of the selected ligands, namely methyl salicylate, α-phellandrene, (1R)-(+)-α-pinene, 3-carene, β-caryophyllene, and α-caryophyllene. Additionally, the behavior bioassays were also showed that D. citri had significant behavioral responses toward to α-caryophyllene, β-caryophyllene, (1R)-(+)-α-pinene, and α-phellandrene. Our investigation infer that the DcitOBP1 protein might play a crucial role in host-plant volatile odorants’ perception in D. citri, and these results also have been supplied previous insight evidence into the physiological functions of the DcitOBP1 protein of D. citri.
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ACS Nano
·
2019
·
IF 13.903
Weidong Nie,Wei Wei,Liping Zuo,Chengliang Lv,Fan Zhang,Gui-Hong Lu,Feng Li,Guanghao Wu,Li−Li Huang,Xiaobo Xi,Hai-Yan Xie
Although adoptive T-cell therapy has been successful in hematological malignancy treatment, its application in solid tumors remains a great challenge. Here, using a pH-sensitive benzoic–imine bond and inverse electron-demand Diels–Alder cycloaddition, we prepared magnetic nanoclusters (NCs) armed with responsive PD-1 antibody (aP), which could then bind onto effector T cells due to their PD-1 expression. After adoptive transfer, the magnetization and superparamagnetism of NCs enabled us to magnetically recruit effector T cells and aP simultaneously to tumor sites with MRI guidance. Owing to the acidic intratumoral microenvironment, the benzoic–imine bond then hydrolyzed, leading to the release of aP. The therapeutic effects of adoptive T cells and free aP could thus be spatiotemporally coupled. As a result, we achieved inhibition of tumor growth with few side effects, demonstrating the great promise of such a chemical approach for safe and high-performance adoptive T-cell therapy against solid tumors.
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BIOCATALYSIS AND BIOTRANSFORMATION
·
2019
·
IF 1.627
Lu Dong,Yongkai Xu,Yun Zhang,Aijun Sun,Yunfeng Hu
Chiral 1-phenylethanol and its ester derivative are important chiral chemicals in diverse industries and the preparation of those optically pure enantiomers is of great importance. One bacterium, Bacillus sp. DL-2, whose extracellular proteases could efficiently asymmetrically hydrolyse (±)-1-phenylethyl acetate, was isolated from the deep sea of the Western Pacific. After the immobilization of extracellular proteases and the optimization of enzymatic reactions, (R)-1-phenylethanol was prepared with the enantiomeric excess (e.e.) being 97% and the yield being 41%, respectively. The optimal resolution reaction condition for the preparation of (R)-1-phenylethanol using immobilized extracellular proteases was found to be 5-mM (±)-1-phenylethyl acetate, 360 mg/mL immobilized extracellular proteases, pH 6.5, and 20 °C for 2 h. (S)-1-phenylethyl acetate was generated through enzymatic kinetic resolution with the e.e. being as high as 99% and the yield being 71%, respectively. The optimal resolution reaction condition for the preparation of (S)-1-phenylethyl acetate was found to be 2.5-mM (±)-1-phenylethyl acetate, 440 mg/mL immobilized extracellular proteases, pH 7.5, and 35 °C for 10 h. Our report is the first report about the kinetic resolution of (±)-1-phenylethyl acetate using proteases and the enantio-preference of the proteases was found to be the same as those of most other esterases/lipases. Also notably, the optical purity of (S)-1-phenylethyl acetate generated through kinetic resolution using the proteases of Bacillus sp. DL-2 was the highest report so far. Proteases from deep-sea Bacillus sp. DL-2 are new contributions to the biocatalyst library for the preparation of valuable chiral alcohols and chiral esters through kinetic resolution.
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KOREAN JOURNAL OF CHEMICAL ENGINEERING
·
2019
·Vol. 36
·期 5
·729-739
·
IF 2.476
Pan Jiaying,Ou Zhimin,Tang Lan,Shi Hanbing
Racemic 1-phenylethylamine was resolved by enantiomer selective acetylation using Fe 3 O 4 -chitosan microsphere (CTS)-glutaraldehyde-lipase in a solvent-free system under an alternating magnetic field. Magnetic chitosan microspheres (Fe 3 O 4 -CTS) were prepared via chemical co-precipitation and cross-linked with lipase using glutaraldehyde to form Fe 3 O 4 -CTS-glutaraldehyde-lipase particles. The magnetic, physicochemical, and textural characteristics of Fe 3 O 4 -CTS-glutaraldehyde-lipase particles were assessed by Fourier transform infrared spectroscopy, X-ray diffraction, and scanning electron microscopy. The optimal immobilization conditions were 2.4 mg/mL lipase, 10 mg/mL Fe 3 O 4 -CTS-glutaraldehyde, pH 8.5, 35 °C, 3 h. The loading amount of lipase and the specific activity got to 132 mg/g carrier and 48 U/g. The optimal reaction conditions of the acylation reaction using Fe 3 O 4 -CTS-glutaraldehyde-lipase were 300 mmol/L 1-phenylethylamine, 150 mg immobilized lipase, 2 mL vinyl acetate, 12.6 ×g rotating speed, 40 °C, 8 h. The activity of the Fe 3 O 4 -CTS-glutaraldehyde-lipase particles and conversion were improved when they were exposed to an external alternating magnetic field. The optimum magnetic field was 12 Gs (500 Hz). The conversion, enantiomeric excess of ( R )- N -(1-phenylethyl)acetamide, and E value reached 41.8%, 98.4%, and 264, respectively. Fe 3 O 4 -CTS-glutaraldehyde-lipase could be reused seven times. A kinetic model of the immobilized lipase-catalyzed resolution of 1-phenylethylamine was set up based on the ping-pong bi-bi mechanism. The kinetic constants were V max =1.62×10 −2 mM/min, K A =2.84×10 −4 mM, and K B =5.8×10 −1 mM. The model data fit well with the experimental data.
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