ENVIRONMENTAL POLLUTION
·
2024
·
IF 7.6
Yingying Liu,Xudong Jin,Yanan Ye,Zeqiong Xu,Zhongkun Du,Huachang Hong,Haiying Yu,Hongjun Lin,Xianfeng Huang,Hongjie Sun
3-bromine carbazole (3-BCZ) represents a group of emerging aromatic disinfection byproducts (DBP) detected in drinking water; however, limited information is available regarding its potential cardiotoxicity. To assess its impacts, zebrafish embryos were exposed to 0, 0.06, 0.14, 0.29, 0.58, 1.44 or 2.88 mg/L of 3-BCZ for 120 h post fertilization (hpf). Our results revealed that ≥1.44 mg/L 3-BCZ exposure induced a higher incidence of heart malformation and an elevated pericardial area in zebrafish larvae; it also decreased the number of cardiac muscle cells and thins the walls of the ventricle and atrium while increasing cardiac output and impeding cardiac looping. Furthermore, 3-BCZ exposure also exhibited significant effects on the transcriptional levels of genes related to both cardiac development ( nkx2.5 , vmhc , gata4 , tbx5 , tbx2b , bmp4 , bmp10 , and bmp2b ) and cardiac function ( cacna1ab , cacna1da , atp2a1l , atp1b2b , atp1a3b , and tnnc1a ). Notably, N-acetyl-L-cysteine, a reactive oxygen species scavenger, may alleviate the failure of cardiac looping induced by 3-BCZ but not the associated cardiac dysfunction or malformation; conversely, the aryl hydrocarbon receptor agonist CH131229 can completely eliminate the cardiotoxicity caused by 3-BCZ. This study provides new evidence for potential risks associated with ingesting 3-BCZ as well as revealing underlying mechanisms responsible for its cardiotoxic effects on zebrafish embryos.
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高影响因子
MICROCHEMICAL JOURNAL
·
2024
·
IF 4.9
Lixia Luo,Jinglin Shui,Yingying Gu,Dandan Wang,Jiangyang Qin,Yanan Wang,Yong Mei
In order to detect the cervical cancer biomarker squamous cell carcinoma antigen (SCCA) in human serum, an electrochemical immunosensor was constructed. Zirconium 1,4-dicarboxybenzene MOF (UiO-66), with a high specific surface area, was coupled with gold nanoparticles (AuNPs), creating the UiO-66@AuNPs nanomaterial. This coupling increased the sensitivity of the electrochemical immunosensor by providing many biological binding sites for antibodies to bind to Au-S bonds. Then, mesoporous carbon (CMK-3) was added to the UiO-66@AuNPs nanomaterial to obtain a CMK-3@UiO-66@AuNPs nanocomposite. This further increased the electron transfer proficiency of the material and enhanced the electrochemical signal. Finally, the sensitive detection of squamous cell carcinoma antigen (SCCA) was made possible by unique immunological responses between antigens and antibodies. Transmission electron microscopy (TEM) analysis of CMK-3@UiO-66@AuNPs revealed embedded surfaces and voids; cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS) confirmed the successful assembly of the electrochemical immunosensor. The developed electrochemical immunosensor demonstrated good linearity (R 1 2 = 0.999) from 0.01 pg/mL to 10,000 pg/mL, which is the concentration range of SCCA in human serum. The limit of detection was 0.0034 pg/mL (at an S/N ratio of 3). The immunosensor exhibited excellent precision, stability and selectivity, with a high rate of recovery (98.26–101.8 %). The experimental validation studies demonstrated that the electrochemical immunosensor was effective for the detection of SCCA in human serum, offering a novel method for the early diagnosis and dynamic surveillance of cervical cancer.
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2025
·
IF 8.5
Kai Yang,Zhenjie Tang,Chong Zhang,Ruide Zhang,Sheng Hu,Changjiang Lv,Jun Huang,Jiaqi Mei,Weirui Zhao,Lehe Mei
Salvianic acid A (SAA) is a catechol compound known for its diverse physiochemical functions and has significant applications in the food and pharmaceutical industries. 4-Hydroxyphenylacetate-3-hydroxylase (4HPA3H) is a critical enzyme for SAA biosynthesis, and improving its activity towards p -hydroxyphenyllactate acid (4HPLA) is essential for highly efficient SAA production in stable biosynthetic pathways. To address this, the distal site and loops of the substrate pocket were modified to improve 4HPA3H catalytic activity towards 4HPLA using computer-aided molecular modification methods. As a result, we identified and mutated two critical sites in Ec HpaB, a 4HPA3H monooxygenase from Escherichia coli : T398, a substrate loop site, and M205, a distal site. The mutants M205F, T398S, and M205F/T398S enabled 2.51-, 2.07-, and 2.20-fold increases in catalytic efficiency ( k cat / K m towards 4HPLA), respectively. Molecular dynamics simulations showed that the overall decreased flexibility of the substrate pocket loop in Ec HpaB might help improve the enzyme's catalytic activity for 4HPLA. Owing to the T398 site in the substrate pocket coming from another monomer, molecular modification of Ec HpaB with multi-monomer interactions was also worthy of attention. These findings not only enhanced the biosynthetic efficiency of SAA but also provided insights into enzyme distal site modification and the engineering of multi-monomer enzymes.
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PETROLEUM SCIENCE AND TECHNOLOGY
·
2025
·
IF 1.4
Airong Li,Pan Liu,Yulong Zhao,Yikai Zou
The exploration of shale gas has increasingly emerged in recent years. In this work, the ionic liquid (IL) of 1-butyl-3-methylimidazole ferric tetrachloride was firstly used as a chemical additive to improve the dissolution of shale and enhance shale gas desorption. The analyses of total organic carbon (TOC), Fourier transform infrared spectroscopy, X-ray diffractometer, X-ray photoelectron spectroscopy, and Brunauer–Emmett–Teller measurement were performed. The results revealed that the TOC content was reduced by 66.2% using a concentration of 0.1 mol/L 1-butyl-3-methylimidazole ferric tetrachloride with a solid-liquid ratio of 1:20 at 60 °C and a duration of 48 hr and the dissolution rate of shale was 18.95 wt%. Inorganic minerals within shale were dissolved, and irregular pores were observed, which improve the permeability of shale and the flow of shale gas. Meanwhile, the characterization of residues in the solution presented that the structures of long carbon chain, carbon-sulfur and amines, amides, or heterocyclic within kerogen may be disrupted, which is beneficial for the shale gas desorption. The isothermal adsorption results proved that the adsorption capacity of methane on the kerogen treated was decreased by 53.16%, which inferred that the desorption ability of shale gas can be improved.
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Molecular Metabolism
·
2024
·
IF 7
Junyong Wang,Yaping Guo,Yunduan He,Yifan Qin,Xiuling Li,Ling Yang,Kangdong Liu,Li Xiao
Objective Nonalcoholic fatty liver disease (NAFLD) is an emerging public health threat as the most common chronic liver disease worldwide. However, there remains no effective medication to improve NAFLD. G protein-coupled receptors (GPCRs) are the most frequently investigated drug targets family. The Regulator of G protein signaling proteins 14 (RGS14), as an essential negative modulator of GPCR signaling, play important regulatory roles in liver damage and inflammatory responses. However, the role of RGS14 in NAFLD remains largely unclear. Methods and Results In this study, we found that RGS14 was decreased in hepatocytes in NAFLD individuals in a public database. We employed genetic engineering technique to explore the function of RGS14 in NAFLD. We demonstrated that RGS14 overexpression ameliorated lipid accumulation, inflammatory response and liver fibrosis in hepatocytes in vivo and in vitro . Whereas, hepatocyte specific Rgs14 -knockout ( Rgs14 -HKO) exacerbated high fat high cholesterol diet (HFHC) induced NASH. Further molecular experiments demonstrated that RGS14 depended on GDI activity to attenuate HFHC-feeding NASH. More importantly, RGS14 interacted with Guanine nucleotide-binding protein (Gi) alpha 1 and 3 (Giα1/3, gene named GNAI1/3 ), promoting the generation of cAMP and then activating the subsequent AMPK pathways. GNAI1/3 knockdown abolished the protective role of RGS14, indicating that RGS14 binding to Giα1/3 was required for prevention against hepatic steatosis. Conclusions RGS14 plays a protective role in the progression of NAFLD. RGS14-Giα1/3 interaction accelerated the production of cAMP and then activated cAMP-AMPK signaling. Targeting RGS14 or modulating the RGS14-Giα1/3 interaction may be a potential strategy for the treatment of NAFLD in the future.
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JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2024
·
IF 5.7
Arong Wang,Cheng Guan,Tieqi Wang,Guangqing Mu,Yanfeng Tuo
Our previous studies have shown that Lactiplantibacillus plantarum DPUL-S164-derived indole-3-lactic acid (ILA) ameliorates intestinal epithelial cell barrier injury by activating aryl hydrocarbon receptor (AhR) and nuclear factor erythroid 2-related factor 2 (Nrf2) signaling pathways and promoting tight junction protein expression. This study further explored the crucial substances of L. plantarum DPUL-S164 in alleviating intestinal barrier damage in mice through a dextran sodium sulfate-induced ulcerative colitis mouse model. Compared to dead L. plantarum DPUL-S164 (D-S164), live L. plantarum DPUL-S164 (S164) and its tryptophan metabolite, ILA, showed an effective ameliorating effect on the intestinal barrier injury of mice treated by antibiotic cocktail and sodium dextran sulfate, suggesting that the crucial substances of L. plantarum DPUL-S164 ameliorating intestinal barrier injury are its extracellular metabolites. Furthermore, S164 and its tryptophan metabolite, ILA, ameliorate intestinal barrier injury and suppress intestinal inflammation by activating the AhR–Nrf2 pathway and inhibiting the nuclear factor kappa-B (NF-κB) pathway. These results suggest that L. plantarum DPUL-S164 ameliorates intestinal epithelial barrier damage in mice, primarily by producing ILA as a ligand to activate the AhR pathway.
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ACS Applied Materials & Interfaces
·
2025
·
IF 8.2
Ke-Juan Meng,Kunyu Xiong,Iftikhar Hussain,Momang Tian,Xinwen Ma,Yuxiang Li,Qi-Long Yan,Kaili Zhang
Advanced energetic composites possess promising properties and wide-ranging applications in explosives and propellants. Nonetheless, most metal-based energetic composites present significant challenges due to surface oxidation and low-pressure output. This study introduces a facile in situ method to develop energetic composites Cutztr@AP through the intermolecular assembly of nitrogen-rich energetic coordination polymers and high-energy oxidant ammonium perchlorate (AP). Morphological analysis reveals the unique structure of Cutztr@AP, where Cutztr is distributed throughout the interior and surface of the AP particles. The nonisothermal thermodynamic analysis reveals a heat release of 2378.2 J g–1 for Cutztr@AP2, outperforming the Cutztr/AP2 achieved through ultrasonic mixing (2000 J g–1). Notably, Cutztr@AP2 exhibits promising combustion and pressure output performances, including a significantly shorter duration, a larger flame area, and higher pressure values. This novel and facile preparation technique and microstructure design approach holds significant promise for high-performance propellants, gas generators, and other related applications.
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INTERNATIONAL JOURNAL OF HYDROGEN ENERGY
·
2024
·
IF 8.1
Ligang Ma,Chao Lin,Wenjun Jiang,Le Xu,Yujie Shao,Tianyu Zhu,Ting Zhao,Xiaoqian Ai,Xiaoshan Wu
In the face of energy scarcity and environmental degradation, photocatalysis emerges as a green and highly efficient method to convert solar energy into a renewable energy source . In this study, CdS nanoparticles were successfully grown in situ along the edges of ZnIn 2 S 4 nanosheets using ultrasound during the synthesis process and the second phase precipitation of In(OH) 3 appeared in the process of preparation. Optimal Cd and S ratios resulted in ZnIn 2 S 4 @In(OH) 3 @CdS heterojunction exhibiting remarkable photocatalytic H 2 production performance compared to standalone ZnIn 2 S 4 and CdS. The internal heterojunction interface within the composite naturally generates an electric field, enhancing carrier separation and facilitating their transport. Additionally, the inclusion of In(OH) 3 further expedites carrier extraction. Ultimately, the mechanism behind the photocatalytic H 2 production was elucidated using the Z-scheme mechanism. This work introduced a novel approach to fabricating multifunctional photocatalysts , presenting a fresh perspective in their design and preparation.
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Advanced Science
·
2024
·
IF 14.3
Qiu-Ling Li,Xin Chang,Yu-Mo Han,Zi-Chao Guo,Yi-Na Liu,Bin Guo,Chang Liu,Bin-Rong Yang,Zhong-Kai Fan,Hu-Lin Jiang,Xin Chang
The limited and backward diagnostic approaches elicit high mortality associated with pulmonary fibrosis (PF) because they fail to identify injury phase of PF. Developing a precisely theranostic nanoplatform presents a promising shortcut to reverse PF. Herein, a specific molecular nanotheranostic (Casp-GNMT), which is triggered by endogenous cysteinyl aspartate specific proteinase-3 (caspase-3), boosts antifibrotic efficacy through bioimaging synergistic with chemotherapy at molecular level, facilitating by ionizable lipid and reactive oxygen species sensitive lipid for precise and manageable therapy. The activation of molecular imaging probe (pCY-pairs) by consumption of endogenous caspase-3 initiates fluorescence resonance energy transfer-guided theranostic pattern, aiming to restore mitochondrial dysfunction-induced oxidative stress and inflammatory responses in alveolar epithelial cells II (AECs II). This process sequentially resists the expression of interleukin-1β and vascular endothelial growth factor receptor through combined with nintedanib, further suppressing abnormal injury of AECs II and persistent migration and proliferation of inflammatory cells. Especially, the homeostasis of injured AECs II diminishes excessive accumulation of transforming growth factor-β to restrain myofibroblasts proliferation and collagen deposition, thereby amplifying the possibility of reversing PF. This theranostic nanoplatform is proposed to provide a prompt and exact approach to enhance diagnostic authenticity and treating efficiency through harnessing endogenous indicator for PF reversal.
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FOOD HYDROCOLLOIDS
·
2024
·
IF 11
Kaixin Li,Xin Yuan,Jiajia Zhao,Jinbo Ren,Lingjun Ma,Xiaojun Liao,Xiaosong Hu,Fang Chen,Junfu Ji
The unique structural of anthocyanins makes them vulnerable to degradation within food processing environments. Proteins commonly coexist with polyphenols in natural food matrices on a non-covalent and covalent basis, serving to stabilize protection for polyphenols. Here, three natural pea-extracted proteins, including albumin, vicilin, and legumin were bound to cyanidin-3-O-glucoside (C3G), and binding modes, conjugate structures and functions were characterized. Isothermal titration calorimetry showed a strongly exothermic interaction between C3G and proteins. The results of sodium dodecyl sulfate-polyacrylamide gel electrophoresis and fourier transform infrared spectroscopy revealed that the C3G-quinone (C3GQ) formed by C3G oxidation established covalent cross-links with C–S and C–N in the amino acid residues of proteins, and could form more large molecular weight aggregates at an addition amount of 1 mM. The free sulfhydryl and amino contents of the three protein conjugates decreased with the addition of C3GQ. Due to the presence of 4–5 times more sulfhydryl groups in albumin as compared to globulin, albumin showed a 20% higher binding capacity for C3GQ than globulin. This consequently led to the highest antioxidant activity in the C3GQ-albumin conjugate. In contrast to native pea proteins, the introduction of covalent modifications induces changes in the secondary structure of the pea proteins. Specifically, the structure of albumin tends to exhibit increased orderliness, while the molecular flexibility of globular proteins was augmented. Thus, the conjugates have enhanced thermal stability. Our results affirmed that the stability of C3G can be more effectively improved through designing it to covalently interact with proteins relative to non-covalent interactions. This study aimed at offering novel theoretical underpinnings for the functional utilization of C3G and pea proteins in the food industry.
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JOURNAL OF FOOD SCIENCE
·
2024
·Vol. 89
·期 6
·3183-3193
·
IF 3.2
Shen Duan,Narantsetseg Byambaakhuu,Na Han,Wenguang Zhang,Wenrui Guo,Chao-Mei Ma
The stems of Cynomorium songaricum are used in traditional Chinese medicine as a tonic and also used locally as a food material and livestock feed. It is known that some of the falvan-3-ol monomers and dimers that entered the milk of dairy sheep fed with C. songaricum stems are biotransformation products of the original flavan-3-ol polymers in C. songaricum stems. This study was performed to investigate the biotransformation process of the flavan-3-ols in dairy sheep and to evaluate the bioactivities. The results showed that procyanidin A2 and epicatechin could be released from the polymeric flavan-3-ols of C. songaricum through rumen microbial metabolism. On traumatic and lipopolysaccharide (LPS)-induced inflammation models of Tg (mpx: EGFP) zebrafish larvae and LPS-induced liver injury models of Tg (fabp10a: DsRed) zebrafish larvae, the milk from sheep fed with C. songaricum stems showed stronger anti-inflammatory and hepatoprotective activities compared to blank milk. The absorbed chemical constituents of C. songaricum stems and the metabolites also exhibited anti-inflammatory and hepatoprotective activities, with the dimeric flavan-3-ols being more effective than the monomers. The milk, the absorbed chemical constituents of C. songaricum stems, and the metabolites alleviated the increased level of reactive oxygen species induced by LPS in zebrafish larvae. Practical Application This study found that C. songaricum stems as livestock feed could produce milk that has a beneficial impact on consumer and livestock health in terms of anti-inflammation and hepatoprotection.
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2024
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IF 7.7
Mingmei Xiao,Jiuhua Zhang,Jiajin Wu,Ning Yuan,Tao Liu,Chunzhi Tang,Nenggui Xu,Qingxin Li,Jinchuan Wu,Shijie Li,Zhuoyue Song
3-Acetylaconitine (AAC) is a commercially analgesic drug for arthritis, however, due to its narrow safety range, its clinical application is limited. The objective of the study was to investigate the combined effect of Cucumaria frondosa polysaccharide (CFP) and AAC in microneedles on knee osteoarthritis (KOA). The characterization of microneedles was conducted and a rat model of monosodium iodoacetate (MIA)-induced KOA was established to evaluate the anti-inflammatory and analgesic activity in this study. The inhibitory effects on cartilage degradation and synovial inflammation were determined by morphological observation of the knee joint, pathological and imaging results. The von Frey pain threshold and hind limb weight bearing test were used to evaluate the effect of microneedles on pain relief. The results showed that microneedles had the mechanical strength to penetrate the skin and dissolved well in the skin for drug delivery within 30 min after patch administration. Furthermore, AAC-MN and CFP-AAC-MN both played roles in relieving pain, reducing cartilage degeneration and inhibiting synovial inflammation in KOA. In summary, CFP combined with AAC loading into microneedles exhibited higher skin penetration and more advantages in inhibiting cartilage damage and inflammation compared to AAC alone.
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危险品化学品经营许可证（带存储）