INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2025
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IF 8.5
Wenxiang Hu,Jiangyu Zong,Jiajun Gong,Rong Xue,Yangbin Lv,Biao Liu,Wenting Dai,Yajun Wang,Chu Chu
Lipase immobilization plays a pivotal role in enhancing enzyme stability, reusability, and practical applicability in ligand fishing, a technique used to identify bioactive compounds from traditional Chinese medicine (TCM). However, conventional methods often lead to significant activity loss due to suffering from enzyme leakage, conformational distortion, and mass transfer resistance. In this study, we present a dynamic defect-engineering (DDE) strategy within zeolitic imidazolate framework-8 (ZIF-8) to overcome these limitations. The DDE process enables adaptive formation of transport channels driven by enzyme–framework interactions, facilitating the migration of lipase from surface adsorption to intracrystalline encapsulation. Brunauer–Emmett–Teller (BET) analysis confirmed successful encapsulation, showing a reduced specific surface area of 565.1 m 2 /g for lipase@ZIF-8, indicative of enzyme incorporation within the defect-engineered framework. This structural optimization significantly improved biocatalytic performance, achieving 82 % activity retention and 65 % immobilization yield, compared to 44 % activity retention in conventional in situ encapsulation. Utilizing the preserved catalytic activity, we established a lipase-based ligand fishing platform, which successfully identified 20 potential inhibitors (10 anthraquinones and 10 flavonoids) from Cassiae Semen and Citri Reticulatae Pericarpium using HPLC-Q-TOF-MS/MS analysis. Overall, this work demonstrates the effectiveness of the DDE strategy in enhancing lipase immobilization within ZIF-8, offering a robust and efficient platform for the high-throughput screening of lipase inhibitors from natural products and advancing the application of enzyme-based ligand fishing in TCM research.
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高影响因子
Small
·
2025
·
IF 12.1
Yan Gao,Xiayu Lia,Shuai Huang,Na Zhao,Jianshe Zhao
Three-dimensionally ordered macro-microporous 3DOM-ZIF-8-based peroxophosphotungstate (DIL-PW 4 ) is constructed to break the limitation of micropores inherent in traditional supported catalysts. The presence of ordered macropores expose more active sites and significantly enhance mass diffusion, thereby boosting catalytic activity, especially in reactions involving larger molecules. The prepared composite DIL-PW 4 @3DOM-ZIF-8 is verified by various characterization techniques and used as a catalyst for the extraction oxidation desulfurization of multi-component simulated fuel. The results showed that heterogeneous catalyst DIL-PW 4 @3DOM-ZIF-8 has a remarkable catalytic effect, with 97.03% total sulfides removal in 120 min. In addition, the stability and reusability of the catalyst DIL-PW 4 @3DOM-ZIF-8 are evaluated as important parameters for industrial applications. In addition, a possible mechanism based on experimental studies are proposed.
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COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
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2025
·
IF 5.4
Yunfei Shen,Huaiqi Shi,Keying Qu,Zuopeng Li,Bolong Yang,Zhansheng Wu
Multifunctional cotton fabric holds significant promise for domestic and healthcare applications. A key challenge remains developing sustainable fabrication routes for mechanically durable cotton textiles integrating intrinsic and photothermally amplified antibacterial functions. Herein, we prepared ZIF-8/67-loaded Ag nanoparticles on cotton fabric using in-situ reduction technology (ZIF-8/67-Ag@CCT). Subsequent dip-coating with polydimethylsiloxane (PDMS) produced ZIF-8/67-Ag@CCT@PDMS, enhancing superhydrophobicity. The modified fabric exhibited exceptional superhydrophobicity (water contact angle: 148.55 ± 0.21°) and UV-blocking capabilities (UPF value: 186). The surface demonstrated excellent self-cleaning properties against dyes and maintained robust stability after 30 washing cycles and immersion in acidic/alkaline solutions (>97.0% antibacterial efficacy retention). Critically, the fabric exhibited excellent biocompatibility, as validated by human induced pluripotent stem cell-derived skin organoid assays showing no adverse effects on cell proliferation, apoptosis, or survival rates. Antibacterial effects originated from the penetration of AgNPs and Ag⁺/Zn²⁺/Co²⁺ ions into bacterial structures, disrupting metabolism via reactive oxygen species (ROS) generation. The multifunctional ZIF-8/67-Ag@CCT@PDMS fabric presents a highly promising solution for durable antibacterial medical textiles, offering combined antibacterial activity, exceptional stability, self-cleaning, UV resistance, and biocompatibility.
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SEPARATION AND PURIFICATION TECHNOLOGY
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2025
·
IF 9
Zhibo Huang,Tiandi Pan,Ning Tang,Fei Sun,Peijian Du,Ni Li
Nanofibrous membranes are considered ideal candidates for water purification. However, the membrane fouling results in an irreversible decline in separation flux, while the slippage of nanofibers leads to an increase in pore size under driving pressure, subsequently inducing a decline in separation efficiency. Rational structure design and material selection are crucial for separation performance improvement. In this study, we report double-layer hierarchical structured nanofibrous membranes that demonstrate simultaneous superhydrophilicity and underwater superoleophobicity for oil-water separation via a combination of electrospinning and seed-mediated solvothermal strategy. The double-layered hierarchical structure comprises an upper layer of ZIF-8@PMIA composite nanofibers and a bottom layer of PMIA nanofibers. Notably, the incorporation of ZIF-8 species confers several advantages, such as increased surface roughness, reduced pore size, and enhanced superhydrophilicity, collectively contributing to superior anti-fouling performance and high separation efficiency. Meanwhile, the bottom PMIA nanofibers, with larger diameters, ensure robust mechanical integrity for the entire membrane. Due to the rational structural design, the membrane achieves outstanding separation performance, including a 99.91 % separation efficiency for diesel-in-water emulsions and an ultrahigh flux of 3821.7 L m − 2 h −1 for dichloromethane-in-water emulsions. Therefore, this membrane has broad application prospects in the oil–water separation field.
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COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
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2025
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IF 5.4
Na Li,Shaobai Wen,Chunwei Yu
Sodium alginate (SA) hydrogels have been extensively utilized for pollutant removal from wastewater due to the distinct properties of hydrophilicity, biocompatibility and reusability. However, the limited functional groups and poor adsorption capacity have hindered their applications. In this study, a novel hybrid hydrogel bead, composed of sodium alginate, attapulgite, and zeolitic imidazole framework-8 (SA/ATP/ZIF-8), was synthesized using a one-pot blending and ion cross-linking method for adsorptive removal norfloxacin (NOR) from aqueous solutions. The incorporation of ATP and ZIF-8 significantly enhanced the thermal stability and anti-swelling properties of the SA hydrogel beads. The effects of solution pH, contact time, initial NOR concentration, temperature, and the presence of coexisting substances on NOR uptakes were systematically investigated. The results demonstrated that the NOR uptake on SA/ATP/ZIF-8 hybrid hydrogel beads was significantly higher than that of the pure SA and SA/ATP hydrogel beads, and the maximum theoretical adsorption capacity of SA/ATP/ZIF-8 for NOR was 1360.67 mg/g at pH 5.0. Furthermore, the adsorption kinetic was well-described by a pseudo-second-order model, while the adsorption isotherm was most accurately represented by the Langmuir-Freundlich model. In tests conducted with Nandu River water, the SA/ATP/ZIF-8 exhibited removal efficiencies of 100 % for NOR at an initial concentration of 0.0015 mmol/L and 71 % at 0.015 mmol/L. Spectroscopic characterization and adsorption performance experiments indicated that the adsorption of NOR onto SA/ATP/ZIF-8 involved multiple mechanisms, including hydrogen bonding, electrostatic adsorption, cation exchange, π-π electron donor-acceptor interaction (EDA), and coordination. Consequently, the remarkable features of SA/ATP/ZIF-8 hydrogel beads highlight their great potential and promising practicability for separation and removal of lower-concentration antibiotics from wastewater.
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高影响因子
Small
·
2025
·
IF 12.1
Qi Jing,Tao Gong,He-Qing Shao,Yu-Jie Wu,Jia-Xing Guo,Kai Ke,Lei Gong,Yong Cao,Zheng-Ying Liu,Wei Yang
Elastomer-based resistive super-thin film strain sensors show great application potentials in electronic skins, human–machine interaction systems, wearable devices for healthcare, and machine learning algorithms. However, it is challenging to accurately monitor the deformation of human body joints and organs with curved surfaces (e.g., knees, throats, finger joints) by only taking advantage of material thickness and elasticity of conventional 2D film strain sensors. Herein, a simple strategy is developed to fabricate conformal elastomeric thin film sensors with periodic 3D microstructure inspired by the ridges and valleys of human skin for accurate signal acquisition. Specifically, an 8-micrometer-thick elastic film strain sensor with 3D microstructure is fabricated via thermoforming followed by in situ chemical growth of silver nanoparticles. The 3D film strain sensors exhibit excellent signal linearity (R 2 = 0.99) and relatively high sensitivity (gauge factor = 14) over a relatively wide strain range (≈43%), with an ultra-low strain detection limit of 0.025%, enabling potential applications in human healthcare monitoring and air leakage detection. Thus, this study unveils a simple methodology to process microstructure-enabled conformable 3D film strain sensors, which show good conformability and multiple mechanical sensing functions for advancing the development of next-generation flexible strain sensors.
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COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
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2025
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IF 5.4
Liangliang Zhao,Mingyang Lin,Hongyan Pan,Zheng Chen,Mingrui Liao,Qian Lin,Song Li
Membrane separation technology encounters significant challenges in achieving simultaneous separation and purification of oily wastewater contaminated with heavy metal ions. Herein, ZIF-8 derived mesoporous carbon etched by oxygen plasma (DMC-OP X ) was used as a functional component for adsorbing heavy metal ions and blended with hydrophilic copolymers PVDF- g -PHEMA into the PVDF casting solution, enabled the fabrication of an advanced nanocomposite membrane (M-H-DMC-OP X ) with separation-adsorption functionality through NIPS method. The characterization results indicate that oxygen plasma etching significantly increases the oxygen-containing groups on DMC-OP X surfaces enhancing hydrophilicity, heavy metal adsorption capacity, and mitigating flux reduction caused by unmodified DMC incorporation. Calculation analyses demonstrate that the oxygen-containing groups on DMC-OP X surface can form hydrogen bonding networks with PVDF- g -PHEMA chains, thereby improving the tensile strength of M-H-DMC-OP X membrane. The one-step separation experiments on oil-water emulsions containing Cu 2 + , Pb 2+ , Cr 3+ , Fe 3+ , and Co 2+ demonstrate that the M-H-DMC-OP 6 membrane exhibits outstanding oil-water separation efficiency and heavy metal removal efficiency, reaching over 99.2 % and 82.4 %, respectively. This work developed a multifunctional separation membrane for complex oily wastewater and established a feasible strategy for improving the water flux of nanocomposite membranes.
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高影响因子
JOURNAL OF NANOBIOTECHNOLOGY
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2022
·Vol. 20
·期 1
·1-18
·
IF 9.429
Chen Xuehua,Huang Yongquan,Chen Hui,Chen Ziman,Chen Jiaxin,Wang Hao,Li Dan,Su Zhongzhen
Background Insufficient radiofrequency ablation (IRFA) can promote the local recurrence and distal metastasis of residual hepatocellular carcinoma (HCC), which makes clinical treatment extremely challenging. In this study, the malignant transition of residual tumors after IRFA was explored. Then, arsenic-loaded zeolitic imidazolate framework-8 nanoparticles (As@ZIF-8 NPs) were constructed, and their therapeutic effect on residual tumors was studied. Results Our data showed that IRFA can dramatically promote the proliferation, induce the metastasis, activate the epithelial–mesenchymal transition (EMT) and accelerate the angiogenesis of residual tumors. Interestingly, we found, for the first time, that extensive angiogenesis after IRFA can augment the enhanced permeability and retention (EPR) effect and enhance the enrichment of ZIF-8 nanocarriers in residual tumors. Encouraged by this unique finding, we successfully prepared As@ZIF-8 NPs with good biocompatibility and confirmed that they were more effective than free arsenic trioxide (ATO) in sublethal heat-induced cell proliferation suppression, apoptosis induction, cell migration and invasion inhibition, and EMT reversal in vitro. Furthermore, compared with free ATO, As@ZIF-8 NPs exhibited remarkably increased therapeutic effects by repressing residual tumor growth and metastasis in vivo. Conclusions This work provides a new paradigm for the treatment of residual HCC after IRFA. Graphical Abstract
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高影响因子
JOURNAL OF PHYSIOLOGY AND BIOCHEMISTRY
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2022
·Vol. 78
·期 4
·777-791
·
IF 5.08
Li Ling,Bai Yu,Du Runyu,Tang Lei,Li Ling
Gestational diabetes mellitus (GDM) is a common pregnancy complication with a high incidence in women. Orphan nuclear receptor NUR77 is involved in regulating glucose metabolism. However, its role in GDM has not been fully elucidated yet. In this study, an animal model of GDM was established by feeding mice with a high-fat diet (HFD) before and during pregnancy. NUR77 expression was abnormally upregulated in placenta tissues of GDM mice. We performed gain- and loss-of-function studies of NUR77 in HTR-8/SVneo cells. Cells were incubated with 1 × 10 −6 M insulin for 48 h to induce insulin resistance (IR). The expression of NUR77 was downregulated in HTR-8/SVneo cells following IR induction. Overexpression of NUR77 promoted cell proliferation, migration, and invasion. Notably, NUR77 promoted glucose uptake and enhanced insulin sensitivity in vitro. NUR77 increased the ratio of p-insulin receptor β (IRβ) Tyr1361 /IRβ, p-insulin receptor substrate (IRS)-1 Tyr612 /IRS-1, p-Akt/Akt and decreased p-IRS-1 Ser307 /IRS-1, as well as lowered the expression of glucose transport protein type 1 (GLUT1) and elevated GLUT4. These results suggest the involvement of IRβ/IRS/Akt/GLUT4 signaling activation in the regulatory effects of NUR77 on IR in HTR-8/SVneo cells. Silencing of NUR77 displayed opposite effects. Besides, NUR77 enhanced the expression of autophagy-related protein Beclin 1 and the ratio of LC3II/LC3I. Further study demonstrated that the inhibitory effect of NUR77 on IR was partially attributed to the activation of autophagy. Therefore, we demonstrate that NUR77 enhances insulin sensitivity in HTR-8/SVneo cells likely through activating IRβ/IRS/Akt/GLUT4 pathway and regulating autophagy.
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高影响因子
COLLOIDS AND SURFACES B-BIOINTERFACES
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2022
·
IF 5.999
Lei Ling,Shu Cai,You Zuo,Meng Tian,Tengfei Meng,Hao Tian,Xiaogang Bao,Guohua Xu
Magnesium (Mg) and its alloys exhibit an excellent prospect for orthopedic clinical application due to their outstanding biodegradability and mechanical adaptability. However, the rapid corrosion rate/latent device-associated infections may lead to a failed internal fixation of Mg-based implants. Herein, a novel composite coating consisted of outer copper-doped zeolitic imidazolate frameworks-8 and inner hydroxyapatite ( [email protected] /HA) was in situ constructed on AZ31B Mg alloy via a two-step approach of hydrothermal treatment and seeded solvothermal method . The results verified that the electrochemical impedance of the obtained Cu 45 @ZIF-8/HA composite coating increased by two orders of magnitude to 6.6013 × 10 4 Ω·cm 2 compared to that of bare Mg alloy. This was attributed to the reduced particle size of ZIF-8 nanoparticles due to the doped copper ions , which could be effectively grown in situ on the micro-nano flower-like structure of the HA-coated Mg alloy. Meanwhile, the [email protected] /HA coating exhibited excellent antibacterial properties due to the release of copper ions and zinc ions from [email protected] 8 dissolved in bacterial culture solution. The ICP results unraveled that the released concentration of copper and zinc ions could enhance the activity of alkaline phosphatase in the appropriate range during MC3T3-E1 cell culture in vitro for 7 days. This research revealed that the preparation of multifunctional metal-organic frameworks coating doped with antimicrobial metal ions via the seed layer solvothermal method was significant for studying the antimicrobial properties , osteogenic performance and corrosion resistance of Mg-based bioactive coatings.
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高影响因子
MOLECULES
·
2022
·Vol. 27
·期 21
·7491
·
IF 4.927
Guo-Ying Chen,Zheng-Ming Qian,Shi-Jun Yin,Xi Zhou,Feng-Qing Yang
In this study, a simple colorimetric method was established to detect copper ion (Cu2+), sulfathiazole (ST), and glucose based on the acetylcholinesterase (AChE)-like activity of zeolitic imidazolate framework-8 (ZIF-8). The AChE-like activity of ZIF-8 can hydrolyze acetylthiocholine chloride (ATCh) to thiocholine (TCh), which will further react with 5,5′-dithiobis (2-nitrobenzoic acid) (DTNB) to generate 2-nitro-5-thiobenzoic acid (TNB) that has a maximum absorption peak at 405 nm. The effects of different reaction conditions (buffer pH, the volume of ZIF-8, reaction temperature and time, and ATCh concentration) were investigated. Under the optimized conditions, the value of the Michaelis-Menten constant (Km) is measured to be 0.83 mM, which shows a high affinity toward the substrate (ATCh). Meanwhile, the ZIF-8 has good storage stability, which can maintain more than 80.0% of its initial activity after 30 days of storage at room temperature, and the relative standard deviation (RSD) of batch-to-batch (n= 3) is 5.1%. The linear dependences are obtained based on the AChE-like activity of ZIF-8 for the detection of Cu2+, ST, and glucose in the ranges of 0.021–1.34 and 5.38–689.66 µM, 43.10–517.24 µM, and 0.0054–1.40 mM, respectively. The limit of detections (LODs) are calculated to be 20.00 nM, 9.25 µM, and 5.24 µM, respectively. Moreover, the sample spiked recoveries of Cu2+in lake water, ST in milk, and glucose in strawberry samples were measured, and the results are in the range of 98.4–115.4% with the RSD (n= 3) lower than 3.3%. In addition, the method shows high selectivity in the real sample analysis.Keywords:zeolitic imidazolate framework-8;acetylcholinesterase;copper ion;sulfathiazole;glucose
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高影响因子
ANALYST
·
2022
·Vol. 147
·期 17
·3917-3923
·
IF 5.227
Feiyan Tang,Qing Hua,Xiaobin Wang,Feng Luan,Li Wang,Yanhong Li,Xuming Zhuang,Chunyuan Tian
As an efficient and easily available pesticide, imidacloprid (IM) has been widely used in agriculture to kill pests. However, threats to the ecological environment are becoming increasingly prominent, caused by abuse and pesticide residues. This paper reports a sensitive electrochemiluminescence (ECL) sensor based on upconverting nanoparticle functional zeolite imidazolate framework (UCNPs@ZIF-8) nanocomposites combined with molecularly imprinted polymers (MIPs) to successfully achieve the quantitative detection of IM. The composites exhibited a certain multi-faceted prismatic structure and the effective binding of UCNPs was demonstrated by characterization technology. In addition, a sensor with MIPs/UCNPs@ZIF-8/GCE as the working electrode exhibited outstanding ECL performance, including a strong and stable signal and excellent selectivity under optimal conditions. This sensor showed a good linear response to IM over a wide concentration range (0.1 ng L−1–1 mg L−1), with a limit of detection as low as 0.01 ng L−1. More significantly, it was successfully applied to the determination of IM concentration levels in food, which provides broad application prospects for the construction of pesticide ECL sensors.
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