MICROCHIMICA ACTA
·
2019
·Vol. 186
·期 2
·1-7
·
IF 5.479
Fu Huiping,Chen Jiaming,Chen Lijuan,Zhu Xi,Chen Zhuling,Qiu Bin,Lin Zhenyu,Guo Longhua,Chen Guonan
A surface-enhanced Raman scattering (SERS) substrate with good flexibility and high water absorbing capacity is reported. It consists of a calcium alginate sponge incorporating gold nanoparticles. These are in close contact with the sponge without the need for amino or sulfhydryl modification. The substrate is capable of detecting the dyes crystal violet (CV) and malachite green (MG) in water directly and rapidly by immersing it into the liquid sample. Preconcentration and separation are not required. The dyes absorbed on the sponge can be detected without drying and thus the whole analytical process can be completed within 3 min. The results show that the lowest detectable concentrations are 0.1 and 0.25 μg⋅L −1 for CV and MG, respectively. This is lower than the minimum required performance limits set by the European Commission and the US EPA. Moreover, MG and CV can be simultaneously detected in liquid samples due to their different SERS bands (at 1216 and 1534 cm −1 , respectively). It should be noted that the molecular structures of MG and CV are very similar. Therefore, the method has a large potential for determination of several analytes simultaneously even in complex sample metrics.
Schematic presentation of the fabrication of a sodium alginate sponge loaded with gold nanoparticles. Gold nanoparticles together with gel-like alginate were freeze-dried to form the sponge. The sponge was cross-linked by CaCl 2 solution and then it was freeze-dried again.
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SENSORS AND ACTUATORS B-CHEMICAL
·
2018
·
IF 5.667
Haicong Shen,Kaixin Xie,Liping Huang,Lei Wang,Jianhua Ye,Meng Xiao,Lei Ma,Aiqing Jia,Yong Tang
Pseudorabies virus (PRV) is a pathogen that causes an acute infectious disease in pigs, which could lead to huge losses to the farming industry. The Bartha-K61 strain of PRV, commonly used as a gE-deleted vaccine, does not always protect against the wild-type virus infection effectively. Therefore, the prompt detection of viral infection in gE-deleted vaccine vaccinated pigs is crucial for in-time measures to prevent the spread of diseases. In this study, we developed a Surface-enhanced Raman spectroscopy(SERS) based lateral flow assay based on antigen-antibody reaction to meet the demand. Our method was rapid (15 min), sensitive (LOD: 5 ng mL −1 ), selective for wild-type PRV detection, and quantitatively or semi-quantitatively (DLR: 41–650 ng mL −1 ) compatible. The detection results from this method were consistent with results from the gE-specific PCR, indicating that this SERS-based lateral flow assay could be used as a new tool to differentially diagnose wild-type PRV and gE-deleted vaccine.
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JOURNAL OF HAZARDOUS MATERIALS
·
2019
·
IF 7.65
Lu-Lu Qu,Zhi-Qin Geng,Wen Wang,Kai-Chun Yang,Wei-Peng Wang,Cai-Qin Han,Guo-Hai Yang,Robert Vajtai,Da-Wei Li,Pulickel M. Ajayan
A three-dimensional (3D) substrate was developed by assembling a monolayer of graphitic carbon nitride (O-g-C 3 N 4 ) on Ag nanorod arrays (Ag NRs) for sensitive and recyclable surface enhanced Raman scattering (SERS) detection. The prepared Ag NRs/O-g-C 3 N 4 substrate not only generated a significant Raman enhancement effect as a result of the strong π-π stacking interaction between O-g-C 3 N 4 and the analytes but also possessed excellent self-cleaning property via visible-light irradiation that was attributed to its outstanding catalytic performance. Highly sensitive SERS detection could be achieved with a LOD of 8.2 × 10 −10 M for R6 G, and the substrate could be used repeatedly for at least four cycles with tolerable intensity attenuation. In addition, the 3D substrate exhibited long-term stability originating from the electron-donor effect of O-g-C 3 N 4 and high reproducibility due to the uniform decoration of O-g-C 3 N 4 on the Ag NRs through the strong interaction. Furthermore, using Ag NRs/O-g-C 3 N 4 , the recyclable detection of antibiotics in a water sample was demonstrated with high sensitivity, which indicates that the 3D Ag NRs/O-g-C 3 N 4 substrate is a promising candidate for eliminating the challenges of single-use SERS substrates and building a portable SERS platform to sense organic molecular species.
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ANALYTICAL CHEMISTRY
·
2019
·
IF 6.35
Qian Yu,Yunqing Wang,Rongchao Mei,Yingchao Yin,Jinmao You,Lingxin Chen
Surface coating determined the sensitivity and stability of surface-enhanced Raman scattering (SERS) tags in bioanalysis. The reported various coatings suffered from the drawbacks of a lack of rigidity, stability, or synthesis versatility. Herein, we demonstrated robust polystyrene (PS) coated SERS tags that could be prepared by an easy and universal approach. Taking advantages of biocompatible, transparent, compact properties of PS shell, the coated tags showed satisfactory sensitivity, biocompatibility, and superior structural stability in cell and in vivo imaging applications. More importantly, the PS coating strategy allowed for the encapsulation of SERS tags encoded with not only thiolated but also nonthiolated Raman reporters without loss of sensitivity, as exemplified in the synthesis of 9 different resonant dye-encoded tags. Moreover, the coating of SERS tags with various kinds of substrates was achieved via the same standard protocol. Comparing with widespread silica coated tags, the PS coated ones were more stable in harsh conditions and had an easily expanded ultrasensitive (resonant) tags library with much lower cost (no need of expensive sulfhydryl/isothiocyano reporters with limited types), illustrating great promise as standard analytical tools of commercialized value for bioanalysis, medical diagnostics, and environmental science studies.
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APPLIED SURFACE SCIENCE
·
2018
·
IF 4.439
Xinna Gao,Xueyan Wang,Zheng Yang,Yuhua Shen,Anjian Xie
A novel multi-functional yolk-shell SiO 2 @TiO 2 /CDs(y-STCs) nanocomposite with interesting structure of SiO 2 cores, porous TiO 2 shell, and cavities was synthesized by using a simple method. The experimental results indicate that the yolk-shell structure and modification of carbon dots (CDs) play important roles in the enhancement of SERS signals and photocatalytic performance. The y-STCs nanocomposite used as the SERS substrate showed excellent efficiency and reproducibility for the detection of rhodamine 6G (R6G). The detection limit of R6G was shown to be as low as 6 × 10 −8 M. Furthermore, the fabricated y-STCs nanocomposite utilized as the photocatalytst for the degradation of dinitraphenols (DNP) can greatly shorten reaction time to 120 min compared with previous studies, and the DNP degradation rate could reach 96% under simulated sunlight irradiation. The y-STCs nanospheres also possessed excellent cycle stability for the photocatalytic degradation of DNP. Thus, the bi-functional y-STCs nanocomposite may have potential applications in the detection and photocatalytic degradation of organic pollutants.
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TALANTA
·
2019
·
IF 4.916
Wenjuan Lv,Chunyan Liu,Yao Ma,Xiang Wang,Juanjuan Luo,Weichun Ye
Nanocomposites of graphene oxide/poly (diallyldimethyl ammonium chloride)/Ag nanoparticles (GO/PDDA/Ag NPs) were constructed via a self–assembly process as a surface-enhanced Raman scattering (SERS) substrate, in which functional macromolecules PDDA were utilized to load GO and support Ag NPs. Fundamental SERS performance of this SERS substrate was evaluated using rhodamine 6G (R6G), which displayed excellent enhancement effect, transferable nature and high stability of the synthesized GO/PDDA/Ag NPs substrate. Furthermore, the synthesized SERS substrate was employed in the sensitive detection of adenine with a linear range of 0.05–1000 μM and low detection limit of 1 nM. Other than the large surface area of GO, multiple-hydrogen bond interactions between adenine and the modified PDDA were another important factor in capturing adenine molecules and enhancing SERS signal. The hydrogen bond interaction was calculated using quantum mechanical calculations. Moreover, determination of adenine in aqueous solutions was achieved with good anti-interference ability against other nucleic bases with similar structures, such as guanine , cytosine and thymine . Therefore, GO/PDDA/Ag can be anticipated to be a potential substrate for label-free, fast and sensitive SERS detection of adenine in the field of bioanalysis .
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NANO
·
2018
·
IF 1.1
Yongfeng Gao,Yuanhui Feng,Lu Zhou,Lucia Petti,Zhe Wang,Jun Zhou,Shusen Xie,Jian Chen,Yanping Qing
Ultrasensitive detection of alpha-fetoprotein (AFP) is critical for the early diagnosis of liver cancer. In this work, a novel surface-enhanced Raman scattering (SERS)-based immunoassay complex has been successfully developed for the detection of AFP by using the Au-Ag alloy nanoparticals and the Ag/AgBr hybrid nanostructure. As the typical bimetal or metal/semiconductor plasmonic materials, besides the strong SERS enhancement characteristics, the Au-Ag alloy nanoparticals exhibit excellent monodispersity and the Ag/AgBr hybrid nanostructure demonstrates good stability. The experimental results show that the SERS-based immunoassay of AFP presents a low limit of detection of 1.86 fg/mL and a broad dynamic range from 2 fg/mL to 0.8 μ g/mL. Furthermore, the clinical applicability of the proposed SERS-based immunoassay has been assessed by the detection of AFP in the human serum samples of cancer patient and healthy person. The test data are consistent well with that of chemiluminescence immunoassay (CLIA) in the relative errors of − 8.82–8.06% and show better detection sensitivity. It reveals that the proposed immunoassay protocol is significant for giving insight into the design of ultrasensitive biosensor and the point-of-care testing of cancers.
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JOURNAL OF COLLOID AND INTERFACE SCIENCE
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2018
·
IF 5.091
Nannan Sun,Kaisheng Yao,Chao Wang,Chenchen Zhao,Weiwei Lu,Shuang Zhao,Huiyong Wang,Jianji Wang
Developing a simple and green route for the controlled synthesis of hierarchical nanomaterials with anisotropic particles as subunits is highly desirable and technically important. Here, a functionalized ionic liquid (FIL) assisted strategy is developed for the preparation of hierarchical Au nanomaterials at room temperature. Various morphologies of Au products, including nanoplate-built flowers, nanobar-built flowers and nanourchins, have been well achieved using the current route in aqueous solutions of imidazolium ILs with different functional groups, such as 1-carboxymethy-3-methylimidazolium chloride ([CMmim]Cl), 1-hydroxylethyl- 3-methylimidazolium chloride ([HEmim]Cl), and 1-(3-aminopropyl)-3- methylimidazolium chloride ([APmim]Cl). The influence of these FILs on the morphology of Au products and their growth process has been studied systematically. It is shown that the functionalized ILs play important roles in the construction of these interesting Au hierarchies. The seed, IL concentration (within a certain range), reaction temperature have negligible influence, but stirring and anions of the ILs have important influences, on the product morphologies. Based on these results, the possible growth mechanism is proposed for the formation of different Au hierarchies. In addition, all the Au samples prepared with the assistance of the functionalized ILs, especially the nanoplate-built flowers, exhibit striking SERS sensitivity and high reproducibility.
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TALANTA
·
2018
·
IF 4.244
Lu Zhou,Ye Liu,Fuyan Wang,Zhijian Jia,Jun Zhou,Tao Jiang,Lucia Petti,Yichen Chen,Qi Xiong,Xiaojun Wang
Prostate cancer (PCa) is a leading cause of cancer-related death among males globally. To date, prostate-specific antigen (PSA), as a typical tumour marker, has been widely used in the early diagnosis of PCa. However, in practical clinical tests, high serum levels of PSA show a high probability for false-positive results, leading to misdiagnoses. In this study, we developed a new classification system for PCa, benign prostate hyperplasia (BPH) and healthy subjects by using a surface-enhanced Raman scattering (SERS)-based immunoassay of multiple tumour markers along with a support vector machine (SVM) algorithm. Silver nanoparticles (AgNPs) as immune probes and SiC@Ag@Ag-NPs SERS as immune substrates were constructed into a sandwich structure to serve as an ultrasensitive SERS-based immunoassay platform of tumour markers. With this assay, the limits of detection for PSA, prostate-specific membrane antigen (PSMA) and human kallikrein 2 (hK2) were as low as 0.46 fg mL −1 , 1.05 fg mL −1 and 0.67 fg mL −1 , respectively. Furthermore, the serum levels of PSA, PSMA and hK2 in clinical samples were successfully detected using the SERS-based immunoassay platform, and correct classifications of PCa, BPH and healthy subjects were feasible with help of the linear SVM algorithm. These results demonstrate the potential for improving the diagnostic accuracy of PCa. Overall, the linear SVM classification model with multiple tumour markers exhibited good classifications of PCa, BPH and healthy subjects, with a PCa diagnostic accuracy of 70% that was significantly superior to that of the linear SVM classification model based only on the serum level of PSA (50%). Therefore, combining the SERS-based immunoassay with pattern recognition technology can allow for comprehensive analyses of the serum levels of multiple tumour markers to effectively improve the diagnostic accuracy of cancer with potential applications in point-of-care testing.
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MICROCHIMICA ACTA
·
2018
·Vol. 185
·期 2
·1-8
·
IF 5.705
Shi Qiaoqiao,Huang Jie,Sun Yaning,Deng Ruiguang,Teng Man,Li Qingmei,Yang Yanyan,Hu Xiaofei,Zhang Zhijun,Zhang Gaiping
The authors describe an ultrasensitive method for simultaneous detection of neomycin (NEO) and quinolones antibiotics (QNS). It is based on the use of (a) two immuno-nanoprobes (a probe for NEO and a probe for QNS), (b) surface-enhanced Raman scattering (SERS) detection, and (c), a portable lateral flow assay (LFA). The two probes consist of gold nanoparticles (AuNPs) conjugated to the Raman active molecule 4-aminothiophenol (PATP), and to monoclonal antibody against NEO (NEO mAb) or against NOR (NOR mAb). Quantitative detection of NEO and QNS was realized via SERS of the PATP-coated AuNPs captured in the test line of a LFA. Under optimized condition, the visual limits of LFA are 10 ng·mL −1 for NEO and 200 ng·mL −1 for NOR, and with LODs down to 0.37 pg·mL −1 and 0.55 pg·mL −1 by using SERS. The NEO test line is not interfered by the NEO analogues gentamycin, streptomycin and tobramycin, but the NOR test line suffers from different degrees of cross-reactivity (CR) to 12 common other QNS, the CRs ranging from 1.5% to 136%. The recoveries of NEO and NOR from spiked milk samples ranged between 86% and 121%, with relative standard deviations (RSD) from 3% to 6%. The method is highly sensitive, accurate and effective. It may be applied to simultaneous detection of NEO and 8 QNS, including NOR, enoxacin, ciprofloxacin, ofloxacin, fleroxacin, marbofloxacin, enrofloxacin, and pefloxacin.
Schematic of a lateral flow assay (LFA) based on an indirect competitive model. By using two test lines, the LFA can detect the neomycin and quinolones antibiotics simultaneously. Based on the surface-enhanced Raman scattering (SERS), the LFA shows high sensitivity to antibiotics with low limit of detection.
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Nanoscale
·
2012
·Vol. 4
·期 16
·5210-5216
·
IF 5.914
Qiao An,Peng Zhang,Jun-Mei Li,Wan-Fu Ma,Jia Guo,Jun Hu,Chang-Chun Wang
Highly active surface-enhanced Raman scattering (SERS) substrates of Ag nanoparticle (Ag-NP) modified Fe3O4@carbon core–shell microspheres were synthesized and characterized. The carbon coated Fe3O4 microspheres were prepared via a one-pot solvothermal method and were served as the magnetic supporting substrates. The Ag-NPs were deposited by in situ reduction of AgNO3 with butylamine and the thickness of the Ag-NP layer was variable by controlling the AgNO3 concentrations. The structure and integrity of the Fe3O4@C@Ag composite microspheres were confirmed by TEM, XRD, VSM and UV-visible spectroscopy. In particular, the Ag-NP coated Fe3O4@carbon core–shell microspheres were shown to be highly active for SERS detections of pentachlorophenol (PCP), diethylhexyl phthalate (DEHP) and trinitrotoluene (TNT). These analytes are representatives of environmentally persistent organic pollutants with typically low SERS activities. The results suggested that the interactions between the carbon on the microsphere substrates and the aromatic cores of the target molecules contributed to the facile pre-concentration of the analytes near the Ag-NP surfaces.
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MICROCHEMICAL JOURNAL
·
2025
·
IF 5.1
Wei Zhang,Qiao Tang,Mengmeng Wang,Pei Liang,Xuejiao Zhang,Kun Pang,Qiang Chen,Xiaoping Yu
In this work, an aptamer sensor (aptasensor) for SERS detection of Vibrio parahaemolyticus was prepared by utilizing flower-shaped silver nanoparticles coated with graphene oxide (Ag@GO) and core–shell Au@Ag nanoparticles embedded with the Raman reporter 4-mercaptobenzoic acid (4MBA). Graphene oxide (GO) was synthesized using an improved Hummers method and wrapped around thiol-functionalized Ag nanoflowers to form the Ag@GO composite. The SERS aptamer sensor with the strongest Raman signal of 4MBA was developed by combining Ag@GO with Au@4MBA@Ag NPs modified by aptamer through hydrogen bonding and π-π stacking interactions. The aptasensor exhibited good repeatability and stability, and demonstrated high specificity. And it showed a good linear relationship for Vibrio parahaemolyticus detection within the concentration range of 10 2 cfu/mL–10 7 cfu/mL. Introducing GO as an internal standard improved the linear regression coefficient (R 2 ) of detection from 0.9885 to 0.9956. Additionally, spiked real samples of fresh shrimp were tested, yielding recovery rates between 94.1 % and 104.7 %, which demonstrate the feasibility and practicality of this method.
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