Frontiers in Microbiology
·
2023
·
IF 5.2
Zhao-xia Luo,Yuting Li,Mei-fang Liu,Rui Zhao
Staphylococcus aureus readily forms biofilms on host tissues and medical devices, enabling its persistence in chronic infections and resistance to antibiotic therapy. The accessory gene regulator (Agr) quorum sensing system plays a key role in regulating S. aureus biofilm formation. This study reveals the widely used fluoroquinolone antibiotic, ciprofloxacin, strongly stimulates biofilm formation in methicillin-resistant S. aureus , methicillin-sensitive S. aureus , and clinical isolates with diverse genetic backgrounds. Crystal violet staining indicated that ciprofloxacin induced a remarkable 12.46- to 15.19-fold increase in biofilm biomass. Confocal laser scanning microscopy revealed that ciprofloxacin induced denser biofilms. Phenotypic assays suggest that ciprofloxacin may enhance polysaccharide intercellular adhesin production, inhibit autolysis, and reduce proteolysis during the biofilm development, thus promoting initial adhesion and enhancing biofilm stability. Mechanistically, ciprofloxacin significantly alters the expression of various biofilm-related genes ( icaA , icaD , fnbA , fnbB , eap , emp ) and regulators ( agrA , saeR ). Gene knockout experiments revealed that deletion of agrC , rather than saeRS , abolishes the ciprofloxacin-induced enhancement of biofilm formation, underscoring the key role of agrC . Thermal shift assays showed ciprofloxacin binds purified AgrC protein, thereby inhibiting the Agr system. Molecular docking results further support the potential interaction between ciprofloxacin and AgrC. In summary, subinhibitory concentrations of ciprofloxacin stimulate S. aureus biofilm formation via an agrC -dependent pathway. This inductive effect may facilitate local infection establishment and bacterial persistence, ultimately leading to therapeutic failure.
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ENERGY & FUELS
·
2023
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IF 5.3
Xianhang Jin,Enze Ren,Min Zheng,Xiurong He,Yun Yang
A CO2 phase change absorbent (CPCA) is of interest because it has the potential to reduce energy consumption for CO2 capture. Controllable phase separation behavior was expected in the development and application of a CPCA. In this study, the effects of CO2 loading under different activators and physical solvents on the phase separation behavior of an activated methyldiethanolamine (MDEA)-based CPCA were investigated. By adjustment of the CO2 loading, controllable phase separation behavior was achieved and the absorbent comprising an activator with more −NH– groups and a physical solvent with low ET(30) was favored to exhibit phase separation. Of special interest was that, with the increasing of CO2 loading, phase behaviors, from the liquid phase to solid–liquid phase and then to the liquid phase, were observed for CPCA comprising MDEA/piperazine (PZ)/N-methyl-2-pyrrolidone (NMP), which was different from all of the other known CPCAs. The solid compound was identified as PZ carbamate by X-ray diffraction (XRD), Fourier transform infrared (FTIR), and elemental analysis, and the liquid-phase samples were detected by 13C nuclear magnetic resonance (NMR). The phase change mechanism of this special phenomenon was proposed.
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Pharmaceutics
·
2023
·Vol. 15
·期 12
·2785
·
IF 5.4
Ziyue Xi,Yingying Jiang,Zitong Ma,Qun Li,Xinran Xi,Chuanyong Fan,Shuang Zhu,Junjie Zhang,Lu Xu
The nano-delivery system with a dual biomimetic effect can penetrate deeper in tumor microenvironments (TMEs) and release sufficient antitumor drugs, which has attracted much attention. In this study, we synthesized erythrocyte-like mesoporous silica nanoparticles (EMSNs) as the core loaded with doxorubicin (DOX) and coated them with calcium phosphate (CaP) and erythrocyte membrane (EM) to obtain DOX/EsPMs. The transmission electron microscopy (TEM), fluorescent co-localization and protein bands of SDS-PAGE were used to confirm the complete fabrication of EsPMs. The EsPMs with erythrocyte-like shape exhibited superior penetration ability in in vitro diffusion and tumor-sphere penetration experiments. Intracellular Ca2+and ROS detection experiments showed that the CaP membranes of EsPMs with pH-sensitivity could provide Ca2+continuously to induce reactive oxide species’ (ROS) generation in the TME. The EM as a perfect “camouflaged clothing” which could confuse macrophagocytes into prolonging blood circulation. Hemolysis and non-specific protein adsorption tests proved the desirable biocompatibility of EsPMs. An in vivo pharmacodynamics evaluation showed that the DOX/EsPMs group had a satisfactory tumor-inhibition effect. These advantages of the nano-erythrocytes suggest that by modifying the existing materials to construct a nano-delivery system, nanoparticles will achieve a biomimetic effect from both their structure and function with a facilitated and sufficient drug release profile, which is of great significance for antitumor therapy.
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FOOD AND AGRICULTURAL IMMUNOLOGY
·
2019
·
IF 2.398
Lipeng Yuan,Bo Liu,Kaidan Yin,Zhen-Lin Xu
In this study, a heterologous competitive indirect enzyme-linked immunosorbent assay (ciELISA) for the determination of estriol (E3) in milk was developed. Rabbit polyclonal antibody specific for E3 was raised by introducing a carboxyl spacer arm from bromoacetic acid of E3 as immunizing hapten. A heterologous ciELISA using a coating hapten containing a carboxyl spacer arm from 6-ketoestriol of E3. The assay exhibited the required sensitivity for detection of E3 with limit of detection of 0.07 ng mL−1 and a working range from 0.41 ng mL−1 to 15.78 ng mL−1. Good recoveries were obtained ranging from 83.2% to 125.8% for E3 spiked milk samples. A satisfactory correlation (R2 = 0.994) between the results of ciELISA analysis and that of LC–MS/MS analysis indicated good accuracy and reproducibility of the proposed method, which was ideally suited as a rapid screening tool for routine E3 monitoring.
研究领域:
生物化学
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分析化学
·
食品科学
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Journal of Energy Storage
·
2023
·
IF 9.4
Mingyue Yu,Xiaozhong Yu,Jiahui Hu,Chengbin Tang,Cong Fan
An organic polymer cathode called poly(anthraquinone- alt -benzene) (PAQB) is in-situ synthesized between the monomers of 2,6-dibromoanthraquinone and 1,4-benzenediboronic acid bis(pinacol) ester based on the matrix of carbon nanotube (CNT), leading to the growth of a polymer on CNT (PAQB@C) and the better formation of electron transportation network. After optimization, the sample (PAQB@C) of PAQB grown on 2 wt% CNT shows the best results in Li-ion batteries. In half cells, PAQB@C shows the average discharge potential around 2.2 V (vs. Li + /Li) and peak discharge capacity of 184 mAh g −1 , stably running for 1000 cycles. In full cells with the Li-intercalated graphite (LiC 6 ) as the anode, PAQB@C can deliver the peak discharge capacity of 187 mAh g −1 cathode and peak energy density of 374 Wh kg −1 cathode . And the full cells can show the long lifespan for over 2500 cycles. To the best of our knowledge, the comprehensive performance of PAQB@C is one of the best organic cathodes reported in Li-ion batteries.
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Developments in the Built Environment
·
2023
·
IF 8.2
Yanchen Oinam,Prabhat Vashishtha,Mandip Dahal,Sukhoon Pyo
This study investigates the potential use of calcined lime mud as an activator in the development of a sustainable, ground granulated blast furnace slag (GGBFS)-based cementless UHPC. The effects of the calcination temperature (550 °C to 850 °C) of lime mud and its weight percentage in the UHPC mixture were evaluated with various tests, such as compressive strength, isothermal calorimeter, and thermogravimetric analysis. Microstructural changes and pore size distribution were also analyzed using XRD, FTIR, and MIP. The results showed that calcined lime mud can be an effective activator in the UHPC mix, and its mechanical properties are influenced by the calcination temperature and weight percentage. The addition of 10% calcined lime mud enhanced the compressive strength of the UHPC up to 150 MPa without negatively affecting its microstructural and hydration characteristics, suggesting that calcined lime mud has the potential to be used as a sustainable alternative to CaO in GGBFS-based cementless UHPC.
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LAB ON A CHIP
·
2023
·Vol. 24
·期 1
·8-19
·
IF 6.1
Jingsong Xu,Xingcheng Wang,Qingyuan Huang,Xiaodong He
The closed-open digital microfluidic (DMF) system offers a versatile and powerful platform for various applications by combining the advantages of both closed and open structures. The current closed-open DMF system faces challenges in scaling up due to electrode structural differences between closed and open regions. Here we developed an adjustable closed-open DMF platform by utilizing the modified slippery liquid-infused porous surfaces (SLIPS) with asymmetric electrowetting on dielectric (AEWOD) as a hydrophobic dielectric layer. The consistent electrode structures of the bottom printed circuit board (PCB) electrode array on both the closed and open regions, and the utilization of a transparent acrylic with floating potential as the top plate allow a low-cost and easily scalable closed-open DMF system to be achieved. The impacts of applied voltage, parallel plate spacing, electrode switching interval, and electrode driving strategies on various droplet manipulations were investigated. The results show that the optimal plate spacings range from 340–510 μm within the closed region. Meanwhile, we also studied the influence of the thickness, geometry, and position of the top plate on the droplet movement at the closed-open boundary. Through force analysis and experimentation, it is found that a thin top plate and a bevel of ∼4° can effectively facilitate the movement of droplets at the boundary. Finally, we successfully achieved protein staining experiments on this platform and developed a customized smartphone application for the accurate detection of protein concentration. This innovative closed-open DMF system provides new possibilities for future applications in real-time biological sample processing and detection.
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Nanoscale
·
2023
·Vol. 16
·期 2
·624-634
·
IF 6.7
Jing Huang,Yuan Liu,Jiandong Wu,Fuping Dong,Chu Liu,Jiawei Luo,Xiangchao Liu,Ning Wang,Lei Wang,Haifeng Xu
Cancer cells disseminate through the bloodstream, leading to metastasis in distant sites within the body. One promising strategy to prevent metastasis is to eliminate circulating tumor cells. However, this remains challenging due to the lack of an active and targeted biomedical tool for efficient cancer cell elimination. Here, we developed a magnetic microrobot by using natural materials derived from the extracellular matrix (ECM) to mimic the ligand–receptor interaction between cancer cells and the ECM, offering targeted elimination of cancer cells. The ECM-mimicking microrobot is designed with a biodegradable hydrogel matrix, incorporating a cancer cell ligand and magnetic microparticles for cancer cell capture and active locomotion. This microrobot was fabricated based on an interface-shearing method, enabling controllable magnetic response and size scalability (30 μm–500 μm). The presented ECM-mimicking microrobot can actively approach and capture single cancer cells and cell clusters under the control of specific magnetic fields. The experiment was conducted in a blood vessel-mimicking simulator. The microrobot demonstrates an outstanding elimination efficacy of 92.3% on MDA-MB-231 cancer cells and a stable transport capability of the captured cells over long distances to a designed recycling site, inhibiting cell metastasis. This magnetic ECM-mimicking microrobot based on a bioinspired binding mechanism represents a promising candidate for the efficient elimination of cancer cells and other biological waste in the blood.
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Journal of Materials Chemistry C
·
2023
·Vol. 11
·期 48
·17106-17114
·
IF 6.4
Junyi Huang,Jianfeng Ding,Furui Tan,Yueyue Gao,Xiayao Lu,Chen Dong,Gentian Yue,Xiaobao Xu,Liming Ding
Lead sulfide (PbS) quantum dots (QDs) have been widely researched for various optoelectronic devices due to their high absorption coefficient and tunable bandgap. In QD thin films, charge carriers are transported via point-to-point hopping that is sensitive to energy disorders, leading to the non-radiative recombination of excitons and carriers. To resolve this problem, we herein introduce a Ti3C2Tx MXene:PbS QD bulk heterostructure (BHJ) with the intermediate halogen bridging at the nano-hybrid interface, which boosts the transfer of charges at the donor/acceptor interface and the transport of electrons via 2D continuous MXene nanosheets. Therefore, we proposed a surface halogen (bromide, Br) bridging to replace the carboxyl and hydroxyl groups on the Ti3C2Tx MXene, improving its electrical coupling with PbS QDs. The Br-MXene and PbS QD nanobuilding blocks show an enhanced coordinated interconnection with each other, which is preferentially used as the light-sensitive layer for photovoltaic-type self-powered infrared photodetectors (PDs) that can generate a high specific detectivity of 9.2 × 1013 Jones and a responsivity of 810 mA W−1 at 808 nm. These efficient self-powered PDs show potential applications in invisible traffic monitoring as well as near-infrared imaging. Our research demonstrates that constructing a strategic BHJ is a promising way to resolve the charge transport bottleneck in colloidal QDs optoelectronic devices.
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INTERNATIONAL JOURNAL OF HYDROGEN ENERGY
·
2023
·
IF 7.2
Yu-Ying Li,Ting-Ting Yao,Xiao-Fang Zhang,Yu-Ting Liu,Xinyuan Wang,Gang-Ping Wu
The gas diffusion layer (GDL) is a key component responsible for mass transfer in the proton exchange membrane fuel cell (PEMFC); however, the conventional commercial GDLs exist with series of disadvantages of the long mass transfer routine, high cost of the substrate layer, and complex preparation processes. To address these problems, we develop a novel substrate-free gas diffusion layer (sfGDL) composed of the whisker carbon nanotubes and Ketjen blacks as a crack-free micrometer network structure. Such a unique sfGDL exhibits a competitively high power density of 1.37 W cm −2 at 100 % relative humidity and 3.77 A cm −2 current density at 0.2 V. Besides, compared with the conventional GDLs, the thickness of sfGDL in this work is significantly reduced by 40 %, indicating great potential for practical applications. This work paves a new way to boost the high performance of PEMFCs.
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JOURNAL OF ALLOYS AND COMPOUNDS
·
2023
·
IF 6.2
Jia Hui Guo,Huan Liu,Yong Wang,YaNan Teng,XingWei Sun,Wei Yan Sun,Chun Ping Li,Jie Bai
The development of efficient, stable and low-cost dual-function electrocatalysts and applicated it to Zn-air batteries are very important for the development of renewable energy storage and conversion technologies. Antiperovskite catalysts have attracted much attention recently due to their advantages of adjustable structure and high element selectivity. In this work, a flexible and adjustable antiperovskite catalyst ZnCCe 3−x Ni x (0 ≤ x ≤ 3) loaded on carbon fiber (ZnCCe 3−x Ni x /CNFs) was prepared by electrospinning technology and used as an air cathode catalyst for rechargeable Zn-air batteries. By doping the X-site with highly conductive transition metal Ni, the electronic structure of the catalyst ZnCCe 3−x Ni x /CNFs was optimized, and the catalytic activity of the antiperovskite bimetallic X-site was tuned to achieve an efficient and synergistic catalytic reaction. Among them, ZnCCe 2.7 Ni 0.3 /CNFs showed excellent OER/ORR bi-functional catalytic activity (Δ E = 0.82 V) and good stability. When ZnCCe 2.7 Ni 0.3 /CNFs is used as the cathode catalyst for rechargeable Zn-air batteries, it shows a power density of 117.99 mW cm −2 , and the cycle stability can be maintained for up to 300 h, indicating its application potential in the field of Zn-air batteries.
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Advanced Healthcare Materials
·
2023
·
IF 10
Wen Zheng,Wenyue Yang,Wenlong Wei,Ziru Liu,Pier-Luc Tremblay,Tian Zhang
Multifunctional hydrogel adhesives inhibiting infections and enabling the electrical stimulation (ES) of tissue reparation are highly desirable for the healing of surgical wounds and other skin injuries. Herein, a therapeutic nanocomposite hydrogel is designed by integrating β-cyclodextrin-embedded Ag nanoparticles ( CD AgNPs) in a polyvinyl alcohol (PVA) matrix enhanced with free β-cyclodextrin (CD) and an atypical macromolecule made of β-glucan grafted with hyaluronic acid (HAG). The main objective is to develop a biocompatible dressing combining the electroconductivity and antibacterial activity of CD AgNPs with the cohesiveness and porosity of PVA and the anti-inflammatory, moisturizing, and cell proliferation-promoting properties of HAG. The last component, CD, is added to strengthen the network structure of the hydrogel. PVA/CD/HAG/ CD AgNP exhibited excellent adhesion strength, biocompatibility, electroconductivity, and antimicrobial activity against a wide range of bacteria. In addition, the nanocomposite hydrogel has a swelling ratio and water retention capacity suitable to serve as a wound dressing. PVA/CD/HAG/ CD AgNP promoted the proliferation of fibroblast in vitro, accelerated the healing of skin wounds in an animal model, and is hemostatic. Upon ES, the PVA/CD/HAG/ CD AgNP nanocomposite hydrogel became more efficient both in vitro and in vivo further speeding up the skin healing process thus demonstrating its potential as a next-generation electroconductive wound dressing.
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