JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
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2025
·
IF 6.2
Zheng Zhang,Tian Zhu,Yang Li,Bin Yu,Haiteng Tao,Haibo Zhao,Bo Cui
As the adsorption receptor of bacteriophage tail protein, bacterial lipopolysaccharide (LPS) is a main culprit responsible for nonalcoholic fatty liver disease (NAFLD) caused by high-fat diets. However, few studies have focused on how the interaction between intestinal bacteriophages and bacterial LPS affects the development and progression of NAFLD. Herein, we determined that excessive fat intake significantly increases the levels of endogenous LPS, while the administration of beneficial metabolites of the intestinal microbiota (specifically butyrate) alleviated hepatic injury in rats. The beneficial mechanism of butyrate was attributed to the reprogramming of the structure of the intestinal DNA virome (primarily, phageome). Butyrate possesses the potential to augment bacteriophagic microbial diversity, thereby potentially facilitating interactions between intestinal bacteriophages and bacterial LPS (in the case of homologous phage), further improving mitochondrial dysfunction and reactive oxygen species production, which, in turn, lowered HepG2 cell damage. Likewise, fecal phage transplantation further confirmed that intestinal phages from rats that received butyrate could effectively interact with bacterial LPS to reduce liver damage in rats. Taken together, modifying the intestinal phageome is a promising treatment option for high-fat diet-related NAFLD.
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JOURNAL OF CONTROLLED RELEASE
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2025
·
IF 11.5
Jinjin Liu,Dan Wang,Shuya Wang,Xiaojing Shi,Tingli Xiong,Yebin Lu,Jianbo Yang,Wei Wei,Xuejun Gong,Qiong Huang,Liandong Ji,Kelong Ai
Acute pancreatitis (AP), a potentially fatal disorder driven by macrophage-associated inflammation, involves mitochondrial reactive oxygen species (ROS) overproduction with pancreatic acinar cells (PACs). This ROS surge damages mitochondria, causing mitochondrial DNA (mt-DNA) leakage and PACs apoptosis. Released mt-DNA then activates the pro-inflammatory cyclic GMP-AMP synthase-stimulator of interferon genes (cGAS-STING) pathway in macrophages, exacerbating disease progression. Critically, while scavenging mitochondrial ROS can halt this cycle, existing mitochondria-targeted drugs fail to penetrate the damaged blood-pancreas barrier (BPB). To overcome this limitation, we developed MTP, a novel dual-targeting nanomedicine synthesized from tannic acid, dopamine, and molybdenum oxides. MTP uniquely achieves dual targeting: actively homing to injured BPB and specifically accumulating within PACs mitochondria. This enables robust mitochondrial ROS scavenging, which protects mitochondrial integrity, reduces mt-DNA release, inhibits PACs apoptosis, and crucially blocks mt-DNA-induced cGAS-STING activation in macrophages, thereby suppressing their pro-inflammatory M1 polarization. By simultaneously interrupting both ROS-mediated PACs damage and macrophage-driven inflammation via this dual-targeting strategy, MTP effectively mitigates AP progression, establishing a breakthrough therapeutic approach.
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ENVIRONMENTAL TOXICOLOGY
·
2021
·Vol. 37
·期 4
·708-719
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IF 4.119
Dan Liu,Ye Cheng,Xueying Mei,Yanzhen Xie,Zhipeng Tang,Jianli Liu,Xiangyu Cao
Acrolein is a ubiquitous environmental pollutant that produced by the incomplete combustion of cigarette smoke, forest fires, petroleum fuels, plastic materials, and cooking fumes. Inhalation is a common form of people exposure to acrolein, increasing evidence demonstrates that acrolein impairs the cardiovascular system by targeting vascular endothelial cells. However, the molecular mechanism of the cytotoxicity of acrolein exposure on vascular endothelial cells remains unclear. This work focused on the toxicity of acrolein on human umbilical vein endothelial cells (HUVECs). The molecular mechanism was studied based on oxidative stress, DNA damage response (DDR), and mitochondrial apoptosis pathways. After HUVECs were treated with 12.5, 25, and 50 μM acrolein for 24 h, cell viability, cell colony formation, mitochondrial membrane potential, and adenosine triphosphate content significantly reduced, and acrolein increased intracellular reactive oxygen species, apoptosis rate, and 8-hydroxy-2 deoxyguanosine (8-OHdG) level. Furthermore, p38MAPK and c-Jun N-terminal kinase signaling pathways were activated in response to oxidative stress. Moreover, acrolein induced G0/G1phase arrest, promoted the expression of γ-H2AX, activated the DDR signaling pathway (Ataxia-Telangiectasia-Mutated [ATM] and Rad-3-related/Chk1 and ATM/Chk2), and triggered the consequent cell cycle checkpoints. Finally, the protein expression of Bax/Bcl-2 and cleaved Caspase-3 was up-regulated, suggesting apoptosis was induced by triggering the mitochondrial apoptosis pathway. All these results indicated that acrolein induced HUVECs cytotoxicity by regulating oxidative stress, DNA damage, and apoptosis. This study provides a novel perspective on the mechanism of acrolein-induced cardiovascular toxicity, it will be helpful for the prevention of acrolein-induced cardiovascular disease.
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Scientific Reports
·
2017
·Vol. 7
·期 1
·1-7
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IF 4.259
Cao Hongmei,Fang Xueen,Liu Peng,Li Hua,Chen Weiwei,Liu Baohong,Kong Jilie
Determination of proteins, especially low-abundance proteins with high sensitivity and specificity, is essential for characterizing proteomes and studying their biochemical functions. Herein, a novel Magnetic-Immuno-Loop-Mediated Isothermal Amplification (Im-LAMP) based on DNA-encapsulating liposomes (liposome-Im- LAMP), was developed for trace amounts of proteins. To the best of our knowledge, this is our first report about the magnetic Im-LAMP approach based on liposomes encapsulated template DNA as the detection reagent. The DNA template was released from liposomes and then initiated an Im-LAMP reaction, generating the fluorescence signal with high sensitivity and rapidity. This technique was applied for the determination of P-glycoprotein as a model protein. It was demonstrated that the technique exhibited a dynamic response to P-glycoprotein ranging from 1.6*10 −2 to 160 pg/ml with a greatly low detection limit of 5*10 −3 pg/ml (5 fg/ml) which is substantially better than conventional enzyme-linked immunosorbent assays (ELISA). This ultra sensitivity was attributed to the LAMP reaction initiated by the enormous DNA targets encapsulated in liposomes. This magnetic liposome-Im–LAMP as an alternative approach is attractive for applications in other low-abundance proteins detection in clinical diagnostics.
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RSC Advances
·
2022
·Vol. 12
·期 46
·30024-30029
·
IF 4.036
Baozhu Zhang,Ziyao Yang,Yuxia Li,Ling Ma,Fenfang Li,Xiuqing Lv,Guangming Wen
A label-free aptasensor has been fabricated in order to detect adenosine triphosphate (ATP) using turn-on fluorescence DNA-Ag NCs. The fluorescence of the DNA-Ag NCs could increase remarkably with the addition of ATP mainly because ATP specifically interacts with its aptamer to change the microenvironment of the darkish DNA-Ag NCs located at one terminus or two termini due to the conformational alteration of the aptamer structure. The proposed sensor can detect ATP in a linear range of 6–27 mM with a good detection limit of 5.0 μM. Additionally, the proposed method succeeded in detecting ATP in fetal bovine serum.
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MICROCHIMICA ACTA
·
2020
·Vol. 187
·期 12
·1-8
·
IF 6.232
Pang Jiawei,Lu Yuexiang,Gao Xinyu,He Liuying,Sun Jingwei,Yang Fengyi,Liu Yueying
A fluorescence method for the determination of inorganic pyrophosphatase (PPase) activity has been established based on copper nanoclusters (CuNCs). The polythymine of 40 mer (T40) acts as a template for the reduction reaction from Cu 2+ to Cu 0 by ascorbic acid (AA). This reaction leads to the formation of fluorescent CuNCs with excitation/emission peaks at 340/640 nm. However, the higher binding affinity between inorganic pyrophosphate (PPi) and Cu 2+ hinders the effective formation of CuNCs. This shows low fluorescence intensity. PPase catalyzes the hydrolysis of PPi into Pi during which free Cu 2+ ions are produced. This facilitates the formation of fluorescent CuNCs. Thus, the fluorescence intensity was restored. The fluorescence enhancement of the system has a linear relationship with PPase activity in the range 0.3 to 20 mU·mL −1 , and the detection limit is0.2 mU·mL −1 . The relative intensity (I/I 0 ) at 640 nm for the analytical solution versus system is also employed to screen the inhibitor for PPase with high efficiency.
Schematic representation of a fluorescent assay for the determination of inorganic pyrophosphatase activity and screening its inhibitor based on single-strand polythymine-scaffolded copper nanoclusters.
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ANALYTICA CHIMICA ACTA
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2011
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IF 4.311
Lizhu Yang,Ying Xu,Xiuhua Wang,Jing Zhu,Renyi Zhang,Pingang He,Yuzhi Fang
We functionalized aligned carbon nanotubes (ACNTs) electrode with a new kind of β-cyclodextrin (β-CD) derivative through diazotization reaction. The resulting β-CD/ACNTs electrode was used to detect DNA hybridization in homogeneous solution based on host–guest recognition technology . In the sensing protocol, one special DNA probe was designed with a stem-loop structure and both ends modified, which we called dually labeled DNA probe (DLP). One end of the DLP was labeled with dabcyl as guest molecule for β-CD/ACNTs electrode capture, and the other end was labeled with a CdS nanoparticle as an electrochemical tag to indicate the occurrence of DNA hybridization. In the absence of the target DNA sequence , the DLP maintains its hairpin structure in solution phase and would not be captured and detected by the β-CD/ACNTs electrode. In the presence of the complementary target sequence, the conformational structure of the DLP was altered and a double-stranded DNA (dsDNA) molecule was formed by the hybridization of DLP and complementary DNA sequence. Consequently, the dsDNA was captured by the β-CD/ACNTs electrode owing to guest–host recognition between β-CD and dabcyl. The electrochemical signal from the CdS nanoparticle–dsDNA/β-CD/ACNTs was then measured. Under optimized detection conditions, the proposed method showed high sensitivity and selectivity with a detection limit of 5.0 × 10 −13 M for complementary DNA sequence.
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MOLECULAR PHYLOGENETICS AND EVOLUTION
·
2004
·
IF 2.826
Joseph Hughes,Alfried P Vogler
We considered the contribution of two mitochondrial and two nuclear data sets for the phylogenetic reconstruction of 22 species of seed beetles in the genus Curculio (Coleoptera: Cuculionidae). A phylogenetic tree from representatives found on various hosts was inferred from a combined data set of mitochondrial DNA cytochrome oxidase subunit I, mitochondrial cytochrome b , nuclear elongation factor 1α, and nuclear phosphoglycerate mutase , used for the first time as a molecular marker . Separate parsimony analyses of each data set showed that individual gene trees were mainly congruent and often complementary in the support of clades but the analysis was complicated by failure of PCR amplification of nuclear genes for many taxa and hence missing data entries. When the four gene partitions were combined in a simultaneous analysis despite the missing data, this increased the resolution and taxonomic coverage compared to the individual source trees. Alternative approaches of combining the information via supertree methodology produced a comparatively less resolved tree, and hence seem inferior to combining data matrices even in cases where numerous taxa are missing. The molecular data suggest a classification of the European species into two species groups that are in accordance with morphological characteristics but the data do no support any of the previously recognised American species groups.
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Cell Reports
·
2025
·
IF 6.9
Xihui Xu,Yue Dou,Shangjun Zhao,Chunfang Zhao,Yahua Chen,Mingyi Jiang,Zhenguo Shen,Chen Chen
Plants deploy a “cry-for-help” strategy to recruit beneficial microbes, thereby enhancing stress resistance and expanding their ecological niches in harsh environments. However, the molecular mechanisms driving this plant-microbe communication remain poorly understood. To uncover the underlying dialogue, we explore interactions between two plant-growth-promoting bacteria (PGPBs) and their host plants, rice and Solanum nigrum , under heavy metal (HM) stress. We identify an interkingdom signaling pathway, characterized by “root reactive oxygen species (ROS)-microbial indole-3-acetic acid (IAA)-root DNA methylation,” which mediates plant-PGPB interactions. Under HM stress, root-derived ROS triggers IAA synthesis in PGPBs, which subsequently suppresses ROS production in the roots, thereby mitigating HM damage in plants. Furthermore, PGPB-derived IAA induces root DNA methylation modifications via ROS-dependent pathways, establishing a cross-kingdom signaling mechanism that links microbial metabolism to plant DNA methylation. This interkingdom signaling pathway is widely observed in the rhizosphere, providing insights into plant-microbe interactions in adverse environments.
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Analytical Methods
·
2026
·
IF 2.6
Ningbo Li,Yongdong Wei,Mengsi Liang,Wenkun Zhang,Jie Qiao
Given that ochratoxin A (OTA) exhibits immunotoxic, teratogenic, and carcinogenic properties, it is essential to monitor its presence in food samples. In the present work, a novel electrochemical aptasensor for OTA detection uses β-cyclodextrin-functionalized graphene (CG) to enhance electron transfer and provide abundant double-stranded DNA (dsDNA) binding sites. The sensor immobilizes OTA aptamers, complementary DNA (cDNA), and ferrocene-labeled dsDNA on a gold nanoparticle-modified electrode. The host–guest interaction between β-cyclodextrin (β-CD) and ferrocene (Fc) stabilizes the sensing layer, while thiolated dsDNA ensures strong attachment to AuNPs. Without OTA, impedance is high and current is low. In the presence of OTA, its high affinity for the aptamer induces conformational changes in dsDNA. Upon applying an oxidative potential, ferrocene is oxidized to Fc+, which, due to reduced hydrophobicity, dissociates from β-CD. This releases dsDNA from the electrode surface, reducing the impedance and enhancing the current response. The sensor demonstrates a robust response across a concentration range of 1 pg mL−1 to 100 ng mL−1, achieving a detection limit of 0.78 pg mL−1. Notably, this method has been successfully applied to detect OTA in wine, yielding high recovery rates of 96.20% to 98.67%. These findings suggest that the MCH/AuNPs/dsDNA/CG/GCE configuration is a promising electrochemical sensor for OTA detection.
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JOURNAL OF FOOD PROCESS ENGINEERING
·
2026
·Vol. 49
·期 1
·e70351
·
IF 2.9
Kai Chen,JianZhi Luo,Bin Xiao,XueWei Yuan,Juan Zhang,Nan Wang,LiQiang Liu,AiKe Li,Ailiang Chen
This study established an optimized CTAB-based DNA extraction method for edible oils, which enables stable and reliable DNA recovery from commercially available edible oils for authenticity identification and adulteration detection. Real-time PCR was used to assess DNA precipitation time and organic solvent extraction, revealing that longer precipitation increased inhibitors, hindering PCR amplification, while organic solvents enhanced efficiency. The method was applied to detect adulteration in commercially available soybean and olive oils. A standard curve based on real-time PCR Ct values demonstrated the ability to detect as low as 10% soybean oil in olive oil, with recovery rates of 92% and 80% for samples spiked with 70% and 30% soybean oil, respectively. The coefficients of variation (CV) were 11% and 14%, within acceptable ranges. This reliable method provides a valuable tool for edible oil authentication and adulteration detection.
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Acta Biomaterialia
·
2026
·
IF 9.6
Renyuan Liu,Yubo Tan,Yuli Jiang,Jingwen Zheng,Wenjie Ni,Sheng Lin,Jing Lin,Dandan Chen,Pengfei Lyu,Yaohui He,Gang Liu,Zhixiang Lu
The escalating challenge of chemoresistance in breast cancer treatment severely limits clinical efficacy, necessitating the urgent development of innovative strategies that synergistically enhance tumor cell eradication and remodel the anti-tumor immune microenvironment. To address this, we developed a D-A structured theranostic probe, 1HA4CD, featuring a dihydroxanthene-fluorophore with diethylamino donor and acrylonitrile/pyridyl acceptors. Upon laser irradiation, 1HA4CD enables spatiotemporally controlled reactive oxygen species (ROS, primarily singlet oxygen, 1 O₂) generation. Crucially, its precise nuclear localization facilitates the induction of high-concentration ROS within the nucleus, causing irreversible oxidative genomic DNA damage. RNA sequencing analysis revealed that the transient nuclear ROS overload not only directly induces DNA double-strand breaks (DSBs) but also inhibits DNA repair pathways, creating a "dual-hit" effect that effectively overcomes the chemoresistance associated with traditional DNA-damaging agents through a nuclear-targeted photodynamic mechanism. DNA fragments released into the cytoplasm post-damage are recognized by the cytosolic DNA sensing machinery, subsequently activating the cGAS-STING signaling cascade, which leads to the systemic activation of both innate and adaptive immune responses. In vivo animal studies demonstrated that 1HA4CD-mediated photodynamic therapy exhibits significant therapeutic efficacy against breast cancer, coupled with a favorable biosafety profile. This research presents a nuclear-targeted molecular tool for photodynamic immune activation therapy and advances the development of combination therapies based on DNA damage-induced immune responses. Statement of Significance Photodynamic therapy (PDT) often suffers from limited efficacy due to insufficient subcellular targeting and the inability to induce systemic anti-tumor immunity, especially in chemoresistant cancers. This work presents 1HA4CD, a nuclear-targeting probe designed to enhance PDT by generating spatiotemporally controlled ROS directly within the nucleus. This approach causes direct DNA double-strand breaks while concurrently inhibiting DNA repair, and further activates the cGAS-STING pathway via damaged nuclear DNA fragments, thereby bridging localized photodamage with systemic immune activation. The resulting “dual-hit” mechanism effectively addresses chemoresistance in breast cancer. By integrating precise subcellular targeting with immunomodulation, this study provides a rational strategy for developing bioactive materials that combine PDT with immunotherap.
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