JOURNAL OF MATERIALS SCIENCE-MATERIALS IN ELECTRONICS
·
2024
·Vol. 35
·期 10
·1-9
·
IF 2.8
Ai Yansong,He Hongshen,Shen Peng,Wang Zhenyang,Zhang Hui,Zhou Yuanming,Mei Fei
Perovskite light-emitting diodes (PeLEDs) have attracted considerable attention in optoelectronic devices due to their excellent performance. Here, the performance of PeLEDs was improved by utilizing l -phenylalanine-doped PEDOT:PSS as hole transport layer (HTL). The optimal device with l -phenylalanine doped HTL shows a maximum luminance of 3800 cd/m 2 and a maximum external quantum efficiency of 2.80%. Compared with the control device using pure PEDOT:PSS HTL, the maximum luminance was increased by 90%, and the maximum external quantum efficiency was increased by 154%, respectively. This is mainly due to the fact that PEDOT:PSS with l -phenylalanine doping optimizes the morphology of the perovskite film and suppresses the exciton quenching at the HTL/perovskite interface. In addition, moderate addition of l -phenylalanine can enhance the hole injection capability of HTL. These experimental results provide proof of amino acid-modified PEDOT:PSS as HTL and a basis for improving the performance of PeLEDs.
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Bioactive Materials
·
2021
·
IF 14.593
Zhongyu Jiang,Xiangru Feng,Haoyang Zou,Weiguo Xu,Xiuli Zhuang
PEGylation has been widely applied to prolong the circulation times of nanomedicines via the steric shielding effect, which consequently improves the intratumoral accumulation. However, cell uptake of PEGylated nanoformulations is always blocked by the steric repulsion of PEG, which limits their therapeutic effect. To this end, we designed and prepared two kinds of poly( l -glutamic acid)-cisplatin (PLG-CDDP) nanoformulations with detachable PEG, which is responsive to specific tumor tissue microenvironments for prolonged circulation time and enhanced cell internalization. The extracellular pH (pH e )-responsive cleavage 2-propionic-3-methylmaleic anhydride (CDM)-derived amide bond and matrix metalloproteinases-2/9 (MMP-2/9)-sensitive degradable peptide PLGLAG were utilized to link PLG and PEG, yielding pH e -responsive PEG- pH e -PLG and MMP-sensitive PEG- MMP -PLG. The corresponding smart nanoformulations PEG- pH e -PLG-Pt and PEG- MMP -PLG-Pt were then prepared by the complexation of polypeptides and cisplatin (CDDP). The circulation half-lives of PEG- pH e -PLG-Pt and PEG- MMP -PLG-Pt were about 4.6 and 4.2 times higher than that of the control PLG-Pt, respectively. Upon reaching tumor tissue, PEG on the surface of nanomedicines was detached as triggered by pH e or MMP, which increased intratumoral CDDP retention, enhanced cell uptake, and improved antitumor efficacy toward a fatal high-grade serous ovarian cancer (HGSOC) mouse model, indicating the promising prospects for clinical application of detachable PEGylated nanoformulations.
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Research
·
2023
·
IF 11
Nian Liu,Lihui Chen,Mingjie Yan,Qian Tao,Jie Wu,Jing Chen,Xiang Chen,Wei Zhang,Cong Peng
Natural killer (NK) cells, as key immune cells, play essential roles in tumor cell immune escape and immunotherapy. Accumulating evidence has demonstrated that the gut microbiota community affects the efficacy of anti-PD1 immunotherapy and that remodeling the gut microbiota is a promising strategy to enhance anti-PD1 immunotherapy responsiveness in advanced melanoma patients; however, the details of the mechanism remain elusive. In this study, we found that Eubacterium rectale was significantly enriched in melanoma patients who responded to anti-PD1 immunotherapy and that a high E. rectale abundance was related to longer survival in melanoma patients. Furthermore, administration of E. rectale remarkably improved the efficacy of anti-PD1 therapy and increased the overall survival of tumor-bearing mice; moreover, application of E. rectale led to a significant accumulation of NK cells in the tumor microenvironment. Interestingly, conditioned medium isolated from an E. rectale culture system dramatically enhanced NK cell function. Gas chromatography–mass spectrometry/ultrahigh performance liquid chromatography–tandem mass spectrometry-based metabolomic analysis showed that l-serine production was significantly decreased in the E. rectale group; moreover, administration of an l-serine synthesis inhibitor dramatically increased NK cell activation, which enhanced anti-PD1 immunotherapy effects. Mechanistically, supplementation with l-serine or application of an l-serine synthesis inhibitor affected NK cell activation through Fos/Fosl. In summary, our findings reveal the role of bacteria-modulated serine metabolic signaling in NK cell activation and provide a novel therapeutic strategy to improve the efficacy of anti-PD1 immunotherapy in melanoma.
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ENVIRONMENT INTERNATIONAL
·
2020
·
IF 7.577
Fangjing Lin,Jianteng Sun,Na Liu,Lizhong Zhu
Polychlorinated biphenyls (PCBs) and their biotransformation products, hydroxylated (OH-PCBs) and methoxylated derivatives (MeO-PCBs), have been detected in the environment and biota, especially crops. However, to date, little information is available on the phytotoxicity and metabolic responses induced by these chemicals in crops. In this study, we exposed rice ( Oryza sative L.) seedlings to 2,3,4,5-tetrachlorobiphenyl (CB-61) and its hydroxylated (4′-OH-CB-61) and methoxylated derivatives (4′-MeO-CB-61) at 0, 10, 50, 100 and 500 μg/L, respectively. After exposure for 14 days, significantly growth inhibition and oxidative damage were observed, among which the toxicities of 4′-OH-CB-61 and 4′-MeO-CB-61 were greater than that of the parent PCBs. Metabolomics analysis indicated that exposure to the three chemicals induced different metabolic responses. 4′-MeO-CB-61 mainly affected the saccharide catabolism, including pyruvate metabolism, the TCA cycle, the transfer of acetyl groups into mitochondria and the Warburg effect, resulting in a greater energy consumption. Moreover, both CB-61 and 4′-OH-CB-61 promoted several amino acid metabolism and fatty acid biosynthesis, thereby alleviating the potential ROS damage. This study for the first time evaluates and reveals the phytotoxicity of OH-PCBs and MeO-PCBs at the metabolic level, which attempts to provide important information for accurately evaluating the environmental risks of PCBs from the perspective of metabolism.
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PubMed
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ENZYME AND MICROBIAL TECHNOLOGY
·
2017
·
IF 2.502
Ren-Chao Zheng,Xiao-Ling Tang,Hui Suo,Li-Lin Feng,Xiao Liu,Jian Yang,Yu-Guo Zheng
Tyrosine phenol-lyase (TPL) catalyzes the reversible cleavage of l -tyrosine to phenol, pyruvate and ammonia. When pyrocatechol is substituted for phenol, l -dihydroxyphenylalanine ( l -DOPA) is produced. The TPL-catalyzed route was regarded as the most economic process for l -DOPA production. In this study, a novel TPL from Fusobacterium nucleatum ( Fn -TPL) was successfully overexpressed in Escherichia coli and screened for l -DOPA synthesis with a specific activity of 2.69 U mg −1 . Fn -TPL was found to be a tetramer, and the optimal temperature and pH for α, β-elimination of l -tyrosine was 60 °C and pH 8.5, respectively. The enzyme showed broad substrate specificity toward natural and synthetic l -amino acids. Kinetic analysis suggested that the k cat / K m value for l -tyrosine decomposition was much higher than that for l -DOPA decomposition, while Fn -TPL exhibited similar catalytic efficiency for synthesis of l -tyrosine and l -DOPA. With whole cells of recombinant E. coli as biocatalyst, l -DOPA yield reached 110 g L −1 with a pyrocatechol conversion of 95%, which was comparable to the reported highest level. The results demonstrated the great potential of Fn -TPL for industrial production of l -DOPA.
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高影响因子
APPLIED CLAY SCIENCE
·
2021
·
IF 5.467
Lei Wang,Haris Muhammad,Minwang Laipan,Xiaohu Fan,Junkang Guo,Yongtao Li
The demand for clean water and soil drives high efficiency, less expensive, and eco-friendly clay-composites as adsorbents for remediation of heavy metals polluted environment. We have developed a new water remediation technique by using an organic-inorganic composite, L-cysteine doped polypyrrole modified bentonite (L-cys/PPy/BT) for removal of Cr(VI) and Mo(VI). The L-cys/PPy/BT showed maximum adsorption capacities of 318.5 and 65.4 mg/g for Cr(VI) and Mo(VI) respectively. For Cr(VI) and Mo(VI) the sorption process on L-cys/PPy/BT followed the Freundlich and Langmuir isotherm models, respectively. Furthermore, the pseudo-second-order kinetic model described the adsorption behavior of Cr(VI) and Mo(VI). Moreover, the thermodynamic parameters indicated that sorption processes were endothermic and spontaneous. Mechanistic assessment revealed that adsorption of Mo(VI) on L-cys/PPy/BT could be attributed to chelation, electrostatic attraction and ion-exchange of Mo(VI) with the imine/amine functional groups in the material's matrix , while electrostatic attraction, ion exchange, chelation and redox reaction played a significant role in the removal of Cr(VI). This research provides a new approach for the use of clay materials as potential sorbents for removal of heavy metal ions in contaminated water .
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Soft Matter
·
2015
·Vol. 11
·期 38
·7502-7506
·
IF 4.029
Yong Shen,Guannan Li,Yinan Ma,Deyang Yu,Jing Sun,Zhibo Li
Two novel thermo-responsive polymer brushes were prepared from L-alanine derivatives using the surface-initiated atom transfer radical polymerization (SI-ATRP) technique. The temperature-induced cell capture and release on both polymer brush modified substrates were further explored.
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PubMed
高影响因子
Agronomy-Basel
·
2022
·Vol. 13
·期 1
·121
·
IF 3.949
Xiaoyun Huang,Hongxing Chen,Hui Chen,Chengwu Fan,Yueying Tai,Xiaoran Chen,Wang Zhang,Tengbing He,Zhenran Gao
The spatiotemporal heterogeneity of rice canopy leaves at different leaf positions is very important for non-destructive monitoring under Cadmium (Cd) stress, and is one of the key problems that need to be solved urgently in modern agriculture. This study aims to explore the temporal and spatial heterogeneity of chlorophyll content and fluorescence in rice canopy leaves under different Cd stress concentrations. The responses of the relative chlorophyll content (SPAD) and Chlorophyll fluorescence (ChlF) parameters to Cd stress in the tillering, heading, and filling stages were analyzed through pot experiments with three Cd concentrations: 0.20 mg·kg−1(CK), 0.60 mg·kg−1(Cd1), and 1.60 mg·kg−1(Cd2). Results showed that the canopy leaf SPAD value increased with increasing Cd concentration. Compared with CK, the leaf SPAD value in the Cd1 and Cd2 treatments were 1.91–3.45% and 5.52–12.01% lower, respectively. The SPAD value of the lower leaves was higher in the tillering stage, while the SPAD value of the upper leaves was higher in the heading and filling stages. The non-photochemical quenching coefficient (NPQ) of the third leaf (D3), the initial fluorescence yield (Fo) of the second leaf (D2), and the maximum photochemical efficiency (Fv/Fm) of photosystem II (PSII) in the tillering stage; the NPQ, Fo, and Fv/Fm of the D3 in the heading stage; and the NPQ, Fo, Fv/Fm, and Fv/Fm of the D2 in the filling stage were more sensitive to Cd stress than the other leaves and ChlF parameters.Keywords:cadmium;rice;relative chlorophyll content;chlorophyll fluorescence;photosynthesis
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高影响因子
CELLULOSE
·
2020
·Vol. 27
·期 14
·8155-8167
·
IF 4.21
Guo Jianwei,Liu Xiao,Han Miaomiao,Liu Yadong,Ji Shengxiang
Abstract An amphoteric metal adsorbent, poly( N -acryloyl- l -histidine)-modified sawdust (SD- g -P(A-His)), is prepared by reversible addition–fragmentation chain transfer (RAFT) polymerization of N -acryloyl- l -histidine from modified sawdust. SD- g -P(A-His) possess imidazolyl and carboxyl groups and shows outstanding adsorption performance for heavy metals. The adsorption capacities of SD- g -P(A-His) are 241.8 and 95.7 mg/g for Pb(II) and Cu(II), respectively. Ten tested metal ions, e.g. Au(III), Cr(III), Cu(II), Fe(III), Ni(II), Pb(II), Pd(II), Hg(II), Mg(II) and Zn(II), can be effectively removed from the multi-metal solution at pH > 2.7, while Au(III) and Pd(II) can be selectively recycled from the multi-metal solution by lowering the pH to ≤ 1.0. The adsorption kinetics of Pb(II) and Cu(II) onto SD- g -P(A-His) fit well with the pseudo-second order model. SD- g -P(A-His) can treat the water with low concentration of metal ions down to sub-10 μg/L level to meet the Quality Standards for Drinking Water of China. Moreover, SD- g -P(A-His) can be recycled and maintain high adsorption efficiency (91.5%) even after 5 cycles. Graphic abstract
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JOURNAL OF SEPARATION SCIENCE
·
2015
·Vol. 38
·期 18
·3279-3287
·
IF 2.737
Ji Li,Xiaoling Hu,Ping Guan,Dongmen Song,Liwei Qian,Chunbao Du,Renyuan Song,Chaoli Wang
In this study, dummy imprinting technology was employed for the preparation of l- phenylalanine-imprinted microspheres. Ionic liquids were utilized as both a “dummy” template and functional monomer, and 4-vinylpyridine and ethylene glycol dimethacrylate were used as the assistant monomer and cross-linker, respectively, for preparing a surface-imprinted polymer on poly(divinylbenzene) microspheres. By the results obtained by theoretical investigation, the interaction between the template and monomer complex was improved as compared with that between the template and the traditional l- phenylalanine-imprinted polymer. The batch experiments indicated that the imprinting factor reached 2.5. Scatchard analysis demonstrated that the obtained “dummy” molecularly imprinted microspheres exhibited an affinity of 77.4 M·10 −4 , significantly higher that of a traditional polymer directly prepared by l- phenylalanine, which is in agreement with theoretical results. Competitive adsorption experiments also showed that the molecularly imprinted polymer with the dummy template effectively isolated l- phenylalanine from l- histidine and l- tryptophan with separation factors of 5.68 and 2.68, respectively. All these results demonstrated that the polymerizable ionic liquid as the dummy template could enhance the affinity and selectivity of molecularly imprinted polymer, thereby promoting the development of imprinting technology for biomolecules.
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JOURNAL OF POLYMER SCIENCE
·
2021
·Vol. 59
·期 24
·3111-3121
·
IF 0
Xu Zhang,Wei Zhao,Chengliang Wang,Lan Cao,Qingfu Wang,Jingjiang Sun
In this work, a series of polyesters with different functional side groups were successfully prepared by Passerini three-component polymerization (P-3CP) in “one-pot” from biorenewable l -glutamic acid ( l -GA)-based monomers. First, the polymerization conditions including monomer feed ratios, solvents and comonomer were systematically studied using Boc-protected l -GA with adipaldehyde and tert -butyl isocyanide. Under the optimal polymerization conditions, a variety of N -substituted l -GA-based monomers as well as functional isocyanides were attempted in the P-3CP to produce polyesters with different functionalities. Moreover, it was found that after removal of pendant protecting groups, the obtained l -GA-based polyester degraded spontaneously into small molecules via 1,5-intramolecular cyclization between the pendant amine groups and the ester groups in polymer backbone. The structures and thermal properties of obtained polymers were determined by 1 H NMR, IR, SEC, MALDI-ToF-MS, DSC, and TGA measurements. Starting from biorenewable l -GA, this approach will provide a facile and straightforward route to produce functional and biodegradable polyesters, thus effectively expanding the range of biodegradable polymers. Graphical
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Science China-Life Sciences
·
2025
·
IF 9.5
Gui Tianxiang,Liu Ying,Fu Mingfeng,Wu Han,Su Pan,Feng Xuhui,Zheng Mengmeng,Huang Zixuan,Luo Xudong,Boron Walter F.,Chen Li-Ming
Nicotinamide adenine dinucleotide (NAD) is well known as a coenzyme involved in many redox reactions in cellular energy metabolism, or as a substrate for many NAD + -consuming enzymes, including those that generate the second messenger cyclic ADP-ribose or deacetylate proteins (e.g., histones). The role of NAD in non-catalytic proteins is poorly understood. IRBIT and L-IRBIT (the IRBITs) are two cytosolic proteins that are structurally related to dehydrogenases but lack catalytic activity. Instead, by interacting directly with their targets, the IRBITs modulate the function of numerous proteins with important roles, ranging from Ca 2+ signaling and intracellular pH (pH i ) regulation to DNA metabolism to autophagy. Among the targets of the IRBITs is the Na + -HCO 3 − cotransporter NBCe1-B, which plays a central role in intracellular pH (pH i ) regulation and epithelial electrolyte transport. Here, we demonstrate that NAD modulates NBCe1-B activation by serving as a cofactor of IRBIT or L-IRBIT. Blocking NAD salvage pathway greatly decreases NBCe1-B activation by the IRBITs. Administration of the oxidized form NAD + enhances, whereas the reduced form NADH decreases NBCe1-B activity. Our study represents the first example in which the redox state of NAD, via IRBIT or L-IRBIT, modulates the function of a membrane transport protein. Our findings reveal a new role of NAD and greatly expand our understanding of NAD biology. Because the NAD redox state fluctuates greatly with metabolic status, our work provides insight into how, via the IRBITs, energy metabolism could affect pH i regulation and many other IRBIT-dependent processes.
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