ACS Applied Materials & Interfaces
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2016
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IF 7.145
Jingjing Zhao,Kun Zhang,Yixin Li,Ji Ji,Baohong Liu
Although fingerprints have been widely used in forensic investigations, low resolution and poor universality are still the main obstacles for the development of fingerprint visualization. In this paper, a facile and universal imaging protocol for latent fingerprints (LFPs) was developed by combining sandwiched SERS probes with the highly sensitive and selective recognition of aptamers. The embedded SERS probes (Au/pNTP/SiO2) successfully avoid the environment interference, ascertaining the stability and reproducibility of Raman signals, and simultaneously improve the efficiency of the fingerprint identification. This approach is operationally simple without complicated pre- or post-treatments. Moreover, the fingerprint images display the high resolution in which third-level details can be clearly identified. This is a general approach and can be used to detect various types of fingerprints, including sebaceous, eccrine, fresh LFPs, and aged LFPs on different substrates (such as smooth, scratching, semiporous, and porous surfaces).
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MICROCHIMICA ACTA
·
2016
·Vol. 184
·期 1
·279-287
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IF 4.831
Wang Rong,Xu Yi,Wang Renjie,Wang Chunyan,Zhao Huazhou,Zheng Xiangquan,Liao Xing,Cheng Li
This article describes a microfluidic SERS chip-based rapid and high-throughput method for the determination of chemical and biological analytes, specifically of melamine. The chip consists of an indium tin oxide (ITO) glass plate modified with silver-gold nanocomposites (Ag-Au NCs) and a polydimethylsiloxane (PDMS) cover plate. The chip has five parallel microfluidic channels. The ITO glass provides electrical conductivity and has a smooth surface which makes it well suited for the deposition of the Ag-Au NC by electrodeposition and galvanic replacement. This technique allows good control of the morphology and composition of the composite. Under optimized conditions, the microfluidic SERS chip provides excellent sensitivity for 4-mercaptobenzoic acid (4-MBA). The detection limit is 0.1 nM and the SERS enhancement factor is 2.7×10 10 . The chip exhibits good stability over time and good reproducibility, with a RSD of 8.6 % between the 5 micro-channels. The chip was successfully applied to the determination of melamine in milk with a 10 nM detection limit and an analytical range from 10 nM to 0.1 mM. It is demonstrated that the microfluidic SERS chip developed here shows great promise for use in biochemical assays.
A microfluidic SERS chip employing gold nanocomposites (Ag-Au NCs) as SERS-active substrate was fabricated. Its SERS enhancement factor is 2.7⋅10 10 . The chip was applied to determinate melamine in milk with detection limit of 10 nM.
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Surfaces and Interfaces
·
2024
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IF 5.7
Xuejiao Wang,Jiayi Dai,Xupeng Zhu,Xueyan Liu,Yufeng Tao,Erjin Zhang,Xudong Ren
The presence of hazardous substance in river water poses a severe threat to both the ecological environment and human health. Therefore, designing and preparing highly sensitive SERS platform for monitoring the hazardous substance is of great significance. Herein, the Ag nanoflowers (NFs)@ZIF-8 core-shell nanocomposite was synthesized and utilized as SERS substrate. Using crystal violet as model molecule, this substrate achieved a detection limit of 10 −10 M and superior uniformity with relative standard deviation of less than 15 %, and the enhancement factor (EF) for crystal violet was calculated about 4.37×10 6 . Besides, the SERS signal only showed a slight attenuation after 3 months storage. As a demonstration, the SERS platform achieved sensitive detection of two hazardous substances thiram and methylene blue with detection limits of 10 −7 M and 10 −8 M. And the recoveries of SERS detection for thiram and methylene blue in river water were between 70 % and 182 %. The electromagnetic enhancement provided by Ag NFs and the enriching effect of ZIF-8 shell collectively contribute to the excellent performance of the Ag NFs@ZIF-8 SERS platform. This work delivers a promising strategy for constructing hybrid SERS platform for highly sensitive detection.
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MICROCHIMICA ACTA
·
2025
·Vol. 192
·期 3
·1-10
·
IF 5.3
Liu Jianghua,Wang Yu,Zhang Xinyue,Huang Mingquan,Li Guoliang
A novel direct lysis method combined with amplification-free CRISPR/Cas12a-SERS genosensor was for the first time developed to rapidly and sensitively identify meat adulteration. Notably, polystyrene (PS) microspheres, with distinct shrinking and swelling properties, were dexterously employed to encapsulate biological-silent Raman reporter 4-mercaptobenzonitrile (4-MBN) and act as a controlled-release signal probe. Target DNA activated the trans -cleavage activity of CRISPR/Cas12a towards ssDNA linked with PS microsphere to liberate the signal probe, which was able to release numerous Raman reporters after treatment with THF solution, resulting in high signal amplification. Through this platform, trace target DNA was deftly transformed into a sensitive Raman signal and could be on-site determined through a portable Raman equipment. Under optimized conditions, this strategy displayed good linearity in the range 1–450 ng/μL ( R 2 = 0.9943) and favorable sensitivity with limit of detection as low as 0.23 ng/μL without any pre-amplification. Moreover, it exhibited good applicability to on-site identification of commercial meat samples in complicated food matrix. In addition, DNA extraction by direct lysis and amplification-free detection realized ultrafast meat adulteration determination within 35 min from sampling to result. This method possessed great potential in rapid and on-site accurate determination of meat authenticity. Graphical abstract
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BIOSENSORS & BIOELECTRONICS
·
2024
·
IF 10.7
Yehang Wu,Pan Li,Tao Xie,Rui Yang,Rui Zhu,Yulong Liu,Shengyu Zhang,Shizhuang Weng
Determination of Fusarium spores is essential for precision control of Fusarium head blight and ensuring agri-food safety. As a highly sensitive real-time detection technique with rich fingerprint information and little influence from water, surface-enhanced Raman spectroscopy (SERS) has been widely applied in the determination of microorganisms. However, fungi determination faces significant challenges including low sensitivity, and poor specificity. The enhanced quasi-meshing hotspot effect (EQMHE) integrated with the embedded attention residual network (EARNet) was proposed to realize a label-free and accurate SERS determination of various Fusarium spores. The EQMHE can promote the binding of spores and nanoparticles to form numerous hotspots, significantly enhancing signal quality and improving the detection limit by at least four orders of magnitude. The key factors inducing EQMHE were validated through various characterization techniques. Moreover, due to its excellent feature extraction and recognition capabilities, EARNet successfully overcomes the limitations of spectral similarity, achieving determination accuracies of 100% in the training set, 98.33% in the validation set, and 100% in the prediction set for three Fusarium species from actual samples. EARNet requires only a small amount of training data and provides rapid and accurate diagnostics. Throughout the process, the spores do not require culturing or lysing, providing an effective determination method for the practical determination of mixed fungal spores. Overall, the proposed strategy effectively addresses challenges such as the need for fungal spore cultivation, difficulties in SERS hotspot formation, and suboptimal signal quality, and it holds significant promise for applications in disease control, food safety, and agricultural production.
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JOURNAL OF MATERIALS SCIENCE
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2024
·Vol. 59
·期 25
·11319-11332
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IF 3.5
Wang Yutong,Liang Xiao,Lv Yiyiwu,Li Xiao,Xia Qinghai,Yang Dezhi,Yang Yaling
This study synthesized Fe-doped dopamine carbon dots (Fe-DACDs) as a reducing agent and template hybrid Au and Pt nanoparticles (Au@Pt/Fe-DACDs). Au@Pt/Fe-DACDs with core–shell structures show strong peroxidase (POD)-like activity at physiological pH. A colorimetric and surface-enhanced Raman scattering (SERS) dual-mode detection system for glucose in human serum was constructed by combining Au@Pt/Fe-DACDs with natural glucose oxidase (GOx). The cascade catalysis reactions convert glucose into hydrogen peroxide (H 2 O 2 ), and then, the Au@Pt/Fe-DACDs as both POD-like nanozyme and Raman-enhancers oxidize colorless and weak Raman-active 3,3′,5,5′-tetramethylbenzidine (TMB) into blue and strong Raman-active ox-TMB. The linear response ranges of glucose are from 0.02 to 1 mM, with a detection limit of 2.3 μM for colorimetric detection and 0.01 mM to 0.7 mM with a detection limit of 1.4 μM for SERS sensors. Glucose content in human serum samples was successfully assayed with a good recovery from 97.6 to 105.3%. Overall, these results demonstrate a new way of designing biological detection systems based on SERS and colorimetric dual-mode sensing.
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CHEMICAL ENGINEERING JOURNAL
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2024
·
IF 13.3
Zhuxu Xu,Adelakun Moses Erinomo,Zhenhua Dan,Fengxiang Qin,Hui Chang
Accurate and sensitive surface-enhanced Raman scattering (SERS) substrates are urgently demanded for the various analytic techniques. The SERS substrates with multimodal and gradient porous structure, utilized for the Rhodamine 6G (R6G) detection, have been designed and successfully fabricated from the thermal diffusion couples of Al and Cu foils via chemical dealloying. The structural inheritance of the initial microstructure of thermal diffusion couple precursors governs the formation of the final layer-by-layer gradient porous Cu with different characteristic pore sizes and morphology. An outmost surface layer of disordered lipstick-like porous structure on the SERS substrates enhances SERS effects as has been expected. The SERS activity of R6G Raman probes on gradient porous Cu is evaluated as a SERS enhancement factor of 6.63 × 10 12 and limit of detection of 2.10 × 10 −17 mol/L, higher than other porous SERS substrates. Meanwhile, the present SERS substrates are flexible and exhibit good bending properties due to the unreacted Cu layer as a supporting skeleton and other gradient porous Cu layers. The superior SERS performances are considered to result from the synergetic effects of the surface scattering of porous Cu rods and enhancements of the high electromagnetic fields between the small gaps between the nanopores.
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Materials Today Communications
·
2024
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IF 3.7
Sihang Zhang,Jiechen Xu,Mingjie Lu,Chunjian Wu,Jiangtao Xu,Zhichang Sun,Lei Ding,Xing Liu,Long Wu,Shouxiang Jiang
Aramid nanofibers (ANFs) have gained great interest as promising polymer substrates for various applications because of their exceptional mechanical properties, high aspect ratio , large specific surface area and excellent chemical and thermal stability. However, the use of ANFs as nano-sized functional substrates for surface-enhanced Raman scattering (SERS) sensing applications has been scarcely reported. In this research, ANF membranes fabricated by vacuum-filtration are served as SERS matrix materials . The silver nanoparticle-coated ANF (AgNP@ANF) membranes are fabricated by a facile, cost-effective, environmental-friendly, and scalable magnetron sputtering technique. Different sputtering times from 15 to 120 s have been studied for AgNP@ANF substrates in terms of their SERS performance. The optimal AgNP-90@ANF SERS substrate exhibits good detection sensitivity of 10 −10 M for malachite green (MG), high enhancement factor of 7.7×10 8 and prominent signal reproducibility (RSD=5.87%), as well as impressive storage stability. Furthermore, the AgNP@ANF SERS substrates also demonstrated excellent detection capability for methylene blue and crystal violet , with sensitivities of 10 −10 and 10 −9 M, respectively. Besides, the flexible AgNP@ANF SERS substrate can also be used to rapidly detect pesticide residues on uneven surfaces through a facile paste-and-read treatment. The simple-to-manufacture, inexpensive, scalable, and sensitive AgNPs@ANF sensors are very promising for commercial SERS applications in the future.
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ANALYTICAL CHEMISTRY
·
2024
·
IF 6.7
Jing Yang,Wei Li,Hao Li,Xiu Wang,Kaisong Xu,Qianjin Li,Tiesong Zheng,Jianlin Li
A high-throughput, rapid, and highly sensitive surface-enhanced Raman spectroscopy (SERS) microarray for screening multiple mycotoxins has been developed on a three-dimensional silver nanoparticle porous silicon (3D AgNP-Psi) SERS substrate, which was easy to be engineered by electrochemical etching and magnetron sputtering technology. The etching current density, etching waveform, and target material for magnetron sputtering have been investigated to obtain an optimal 3D SERS substrate. The optimized 3D AgNP-Psi SERS substrate showed an enhancement factor of 2.3 × 107 at 400 mA/cm2 constant current density etching for 20 s and Ag target magnetron sputtering for 200 nm thickness on the surface of Psi. The simulation electric field distribution showed the near-field enhancement can reach 3× higher than that of AuNPs. A protein microarray has been designed to screen multiple mycotoxins by AuNP Raman tags and a competitive immunoassay protocol on the surface of the 3D SERS substrate. The SERS protein microarray displayed wide linear detection ranges of 0.001–100 ng/mL for ochratoxin A, 0.01–100 ng/mL for aflatoxin B1, 0.001–10 ng/mL for deoxynivalenol, along with pg/mL low limit of detection, good recovery rates, repeatability, and reproducibility. The 3D SERS protein microarray is easily engineered and has a great potential application in medicine, environment, and food industry fields.
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SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
·
2024
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IF 4.3
Jin-Yu Zhou,Jian Zhu,Guo-Jun Weng,Jian-Jun Li,Jun-Wu Zhao
A novel surface-enhanced Raman scattering (SERS) composite substrates on the basis of Ag triangular nanoplates(Ag TNPs)-modified SiO 2 photonic crystals (PC) is fabricated and applied to the SERS detection of malachite green (MG). It consists of uniformly arranged Ag TNP@SiO 2 , a new PC. Notably, Ag TNP are uniformly aligned on the SiO 2 surface, forming a three-dimensional high-density hotspot nanostructure. With the tip “hot spots” of Ag TNPs, Bragg diffraction of SiO 2 and coupling enhancement between Ag TNPs and SiO 2 , the SERS enhancement of this composite substrates was multiplied. The effect on the SERS of Ag TNP@SiO 2 composite substrate was systematically optimized by tuning Ag TNP size, size of SiO 2 microspheres, coverage of Ag TNPs on SiO 2 and fabrication method of Ag TNPs and PC. Moreover, the uniform of SERS composite substrates and Raman signal was dramatically increased by the method of vertical deposition. Eventually, the SERS composite substrates were employed in MG detection. Its broad detection range of 1 pM–1 μM and low limit of detection (LOD) of 0.49 pM indicated acceptable sensitivity and repeatability. This work illustrates the promising applicability in food safety analysis based on SERS composite substrates composed by Ag TNP@SiO 2 with numerous SERS enhancements and excellent stability.
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2024
·
IF 7.7
Junjie Zhou,Wenhua Gao,Jinglin Wu,Zhouyang Xiang,Jinsong Zeng,Bin Wang,Jun Xu
Cellulose nanofibrils (CNFs) can serve as an efficient surface enhanced Raman scattering (SERS) platform for in situ detection of trace targets. In this study, a highly reproducible SERS platform based on TEMPO-oxidized CNFs (T-CNFs) was fabricated by the ion-exchange. Self-assembly of silver nanoparticles (AgNPs) was accomplished in only 120 s. The abundant carboxylate groups and good hydrophilicity of T-CNFs facilitated uniform and dense loading of AgNPs over the surface area. The obtained SERS substrate greatly enhanced the Raman signal of different pesticides, and the detection limits of thiram and thiabendazole were 5.81 × 10 −8 M and 9.63 × 10 −8 M, respectively. SERS substrate could produce homogeneous Raman-enhanced signals (relative standard deviation (RSD) = 6.59 %). In addition, due to the good flexibility, SERS substrate could collect and detect pesticide residues from the surface of apples. The intensities of Raman characteristic peak at 1384 cm −1 showed a good linear relationship with the analyte concentrations (0.96 ng/cm 2 –9600 ng/cm 2 ). The constructed SERS substrate provided a theoretical basis for the preliminary rapid screening of hazardous chemical residues in food, which was of great value for the SERS technique to become a routine on-site analysis method for pesticide residues.
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TALANTA
·
2025
·
IF 6.1
Xin Liu,Shiyue Niu,Bin Yang,Jia Liu,Liqian Niu,Xian Wang,Daqian Song,Shuyun Bi
Ultrasensitive Surface-enhanced Raman spectroscopy (SERS) method for the detection of diquat/dipterex was established using bovine serum albumin (BSA)-protected silver nanoparticles (AgNPs) modified MIL-53(Al) (named as BSA/MIL-53(Al)/AgNPs). Compared with unmodified AgNPs, BSA/MIL-53(Al)/AgNPs significantly enhanced the Raman signals of diquat and dipterex and the enhancement factors (EFs) were 1.58 × 10 7 and 2.34 × 10 7 , respectively. The TEM, XRD, TGA, XPS, UV–vis and FT-IR were utilized to characterize BSA/MIL-53(Al)/AgNPs and the binding of the substrate with diquat/dipterex. The optimal measurement conditions were investigated in detail by single factor experiment and response surface model. The impacts of common pesticides and coexisting substances on the determination of diquat/dipterex were studied. Under optimum conditions, linear calibration curves for detecting diquat/dipterex were established with a limit of detection (LOD) of 0.17/0.89 pmol L −1 (3 S 0 / S ). The SERS approaches were used to detect diquat and dipterex in several fruits and vegetables. The recovery was 97.10 %–104.82 % with the relative standard deviation (RSD) of 1.04 %–4.15 % ( n = 5).
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