Journal of Functional Foods
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2021
·
IF 4.451
Xiaolan Chen,Bingyan Cai,Jing Wang,Zhicun Sheng,Haifeng Yang,Dada Wang,Jiahao Chen,Qingqing Ning
Mulberry leaf polysaccharide (MLP) possess many biological characteristics, such as immunomodulatory and antioxidant properties, but whether MLP can impact the distribution and characteristics of the gut microbiota is not yet clear. In this study, the effects of MLP on diversity- and immune-related factors and short-chain fatty acids (SCFAs) of the gut microbiota in mice were investigated. The results showed that MLP could restore the indexes of the thymus and spleen, the damaged intestinal barrier and levels of inflammatory cytokines in cyclophosphamide (CTX)-treated mice, and alleviate oxidative damage to the liver. Additionally, MLP were associated with a relatively higher abundance of Bacteroidetes but lower levels of Firmicutes, Butyricimonas and Eubacterium in CTX-treated mice. Moreover, MLP restored the levels of acetic acid, propionic acid, and n-butyric acid reduced by CTX. These results indicated that MLP, as a special ingredient, might regulate the immune response by altering the spatial structure of the gut microbiota and the production of SCFAs.
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Food Frontiers
·
2021
·Vol. 2
·期 2
·208-217
·
IF 0
Huiting Lin,Jiawen Zhang,Shiyang Li,Baodong Zheng,Jiamiao Hu
The current study was aimed to explore the beneficial effects of p olysaccharides isolated from Laminaria japonica (LP) on gestational diabetes mellitus (GDM) mice. The obtained results demonstrated that the LP improved serum biochemical index, body weight index, and glucose tolerance. Furthermore, the evidence also suggested that the beneficial effects of LP might be attributed to the alternation in gut microbiota by LP supplementation. Particularly, the Turicibacter , a short-chain fatty acids-producing bacterium, was found to be up-regulated. In conclusion, the obtained results indicated that the LP might serve as prebiotic, and might have a great potential to be used as food supplementation for GDM patients.
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EUROPEAN JOURNAL OF PHARMACEUTICAL SCIENCES
·
2021
·
IF 4.384
Yu Cai,Qi Tian,Chao Liu,Liang Fang
The purpose of present study was to develop a long-acting drug-in-adhesive patch of rivastigmine (RVS) to achieve controlled release under high drug loading. Formulation factors including ion-pair, pressure sensitive adhesive (PSA), drug-loading and permeation enhancers were investigated through in vitro skin permeation experiments. Optimized patch was evaluated by pharmacokinetic study. The mechanism of controlled release was studied by FTIR, Raman, DSC , rheology study and molecular modeling . The optimized patch composed of RVS-SA (equal to 30% RVS), 15% POCC as permeation enhancer and AAOH as PSA matrix. The RVS in optimized patch was basically permeated at a uniform rate, and the ratio of the skin permeation amount (2803.38 ± 153.85 μg/cm 2 ) in 72 hours to that of the control group (1000.89 ± 62.45 μg/cm 2 ) was 2.8. The plasma concentration of RVS was stable for 72 hours in vivo ( AUC optimized = 5721.30 ± 1994.87 h ng/mL, MRT 0-t = 29.55 ± 2.49 h), and C max was significantly controlled. The results of the study on the controlled release mechanism showed that the addition of counter ion formed hydrogen bonds with RVS and PSA respectively, which reduced the fluidity and molecular mobility of PSA, and enhanced the interaction between RVS and PSA, thus achieving the purpose of long-acting effect. In conclusion, long-acting drug-in-adhesive patch of RVS was developed, and provided a new idea for the long term drug delivery of Alzheimer's disease .
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高影响因子
TALANTA
·
2021
·
IF 6.057
Hua-Ming Xiao,Yan Shi,Yong-Xue Yang,Shuai Zhao,Xian Wang
The free fatty acids that contain one to eight carbons (C 1 –C 8 ) in biodiesel would affect the quality of biodiesel. It is still a matter of challenge to simultaneously determine the composition of C 1 –C 8 fatty acids in seed oil and seed oil-based biodiesel. Herein, a novel method of charge derivatization coupling with direct infusion mass spectrometry (CD-DIMS) was developed for the determination of the C 1 –C 8 fatty acids in biodiesels. A fixed-charge derivatization reagent, 1-cyclohexyl-3-(2-morpholinoethyl)carbodiimide (CMCT), was used to convert fatty acids into their cationic derivatives, which significantly improved the sensitivity and selectivity of detection. Good linearity was observed with the limits of detection (LODs) in the range of 0.0002–0.001 μg mL −1 for the investigated fatty acids. The recovery was in the range of 85.1%–101.9% and the matrix effect was within the range of 75.5–93.2%. The developed method was carried out to analyze C 1 –C 8 fatty acids in rubber seed oil (RSO) and RSO-based biodiesels produced by different catalysts, including NaOH, TiO 2 , and carbodiimide . It was also applied to the dynamic monitoring of C 1 –C 8 fatty acids in RSO and produced RSO biodiesels during the oxidation process. As results, formic acid , acetic acid, and propionic acid were detected in aged RSO and biodiesel samples. The contents of formic acid, acetic acid, and propionic acid all increased in aged RSO and biodiesels, but with different growth rates. These results demonstrated that the developed CD-DIMS method can provide a quick, accurate, and sensitive analysis of C 1 –C 8 fatty acids in seed oil and biodiesel samples.
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Journal of Functional Foods
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2020
·
IF 3.701
Peixin Wang,Hongliang Zeng,Shaoling Lin,Zhigang Zhang,Yi Zhang,Jiamiao Hu
Hairtail ( Trichiurus lepturus ) is a nutritious marine product that has attracted increasing attention due to its bioactive compounds. In this study, the anti-fatigue properties of hairtail hydrolysate were evaluated by exhaustive swimming experiments. Results indicate hairtail peptide supplementation increased the exhaustive weight-loaded swimming time. Furthermore, decreases in blood lactic acid (BLA), blood urea nitrogen (BUN), creatine kinase (CK), and malondialdehyde (MDA) content; and increases in glycogen content and antioxidant enzymes activities were observed in mice receiving hairtail peptides treatment after a 30 min swimming test. Pearson correlation analysis suggests fatigue indexes, including energy sources (hepatic glycogen and muscle glycogen content), metabolites (BLA and BUN content), and CK content significantly correlated with antioxidant levels (glutathione peroxidase (GSH-Px), superoxide dismutase (SOD), and catalase (CAT) activities and MDA content) and fecal SCFA content (propionic acid and butyrate acid content). In conclusion, long-term supplementation with hairtail peptides may improve exercise performance by relieving fatigue.
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Food & Function
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2020
·Vol. 11
·期 10
·9216-9225
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IF 4.171
Junhong Lan,Kangli Wang,Guangyong Chen,Guangtian Cao,Caimei Yang
The prebiotics inulin (INU) and isomalto-oligosaccharide (IMO) influence intestinal health and immunity, but their effects on constipation are not clearly established. We evaluated the effects of INU and IMO in a rat model of diphenoxylate-induced constipation. Twenty-four male rats were divided into four groups: basal diet (Con), 40 mg kg−1 diphenoxylate (PCon), 20 g kg−1 INU and treated with 40 mg kg−1 diphenoxylate, and 20 g kg−1 IMO and treated with 40 mg kg−1 diphenoxylate. INU and IMO increased the number, weight, and water content of fecal pellets, and decreased the time to the first black stool in rats with constipation. Serum levels of the gastrointestinal motility-related hormones adrenocorticotropic hormone (ACTH), motilin (MTL), and Substance P (SP) were higher and corticosterone (CORT), vasoactive intestinal peptide (VIP), and calcitonin gene-related peptide (CGRP) were lower in rats treated with prebiotics than in untreated rats. Colon tissue levels of MTL and SP were increased, and VIP and CGRP were decreased by prebiotics. Furthermore, in rats with constipation, INU and IMO increased the colonic contents of short-chain fatty acids. The relative abundance of Bacteroidetes was lower in the prebiotics groups than in the Con and PCon groups. Lactobacillus was more abundant in the INU and IMO groups than in PCon rats. Lactobacillus reuteri and Lactobacillus intestinalis were more abundant in the IMO group than in the PCon group (P < 0.01), and L. intestinalis was more abundant in the INU group than in the PCon group (P < 0.01). In summary, INU and IMO improved constipation and altered the intestinal microbiota in a rat model of constipation.
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ANALYST
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2021
·Vol. 146
·期 6
·1996-2008
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IF 4.616
Taotao Fan,Zongcheng Yan,Chanyuan Yang,Shunguo Qiu,Xiong Peng,Jianwei Zhang,Lihua Hu,Li Chen
A series of natural, environmentally friendly and low-cost menthol-based hydrophobic deep eutectic solvents (DES) were synthesized to extract and concentrate solutes from dilute aqueous solutions, especially triphenylmethane (TPM) dye micropollutants. The system has excellent extraction performance for TPM. Density functional theory (DFT) and molecular dynamics (MD) simulation were used to quantitatively analyze the effect of the DES composition and TPM structure on the distribution of target molecules in two phases. The solvation free energy of ethyl violet (EV) in DES (−17.128 to −21.681 kcal mol−1) is much larger than that in water (−0.411 kcal mol−1), and increases with the increase of the HBD chain length, which is proportional to the extraction rate, indicating that the TPM molecules are more inclined to the DES environment, especially long-chain DES, than aqueous solution. For the same C12DES, the extraction efficiency of the TPM dyes follows the order: ethyl violet (EV) (99.9%) > crystal violet (CV) (99.6%) > methyl violet (MV) (98.8%). EV has the smallest positive charge and the smallest dipole moment (9.109 D), and the Flory–Huggins parameters of EV (χEV–C12DES 0.053) relative to MV and CV are the smallest in C12DES, and are also the largest in water (χEV–H2O 0.053), indicating that EV has the largest polarity difference with H2O and is more easily detached from water and compatible with the long-chain DES phase. The motion of EV and MV on the phase interface of DES and water was calculated to further analyze from the molecular level. At the same time, EV tends to move into the DES phase. In summary, the excellent extraction ability of DES for TPM is verified through experiments and simulations, providing solid theoretical support in terms of separation in other fields.
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EUROPEAN JOURNAL OF PHARMACEUTICAL SCIENCES
·
2020
·
IF 3.616
Xiaoyu Zhang,Junping Zheng,Nan Jiang,Guangjun Sun,Xinkun Bao,Mingwang Kong,Xue Cheng,Aizhen Lin,Hongtao Liu
Lactulose is a common laxative and has been widely applied to clinical treatment for constipation. This study aimed to explore the improving effect of lactulose on constipation through the mediation of gut microbiota and intestinal metabolites. BALB/c mice with constipation induced by loperamide were orally treated with lactulose for four weeks. After the treatment, the constipation-related factors were determined. The effect of lactulose on the composition of gut microbiota was assessed by 16S rDNA gene sequencing. Gas chromatography or liquid chromatography-mass spectrometer (GC/LC-MS) analysis was used for the quantification of intestinal metabolites. The treatment of constipated mice with lactulose accelerated intestinal motility, suppressed inflammatory responses, protected gut barrier, and improved metabolisms of water and salt in the intestinal tract. These therapeutic effects were attributed to the reversed gut microbiota dysfunction, which conferred the benefit to the production of intestinal metabolites including bile acids, short-chain fatty acids, and tryptophan catabolites. Further, the depletion of intestinal flora from loperamide- or (loperamide + lactulose)-treated mice confirmed the significance of gut microbiota in the mediation of constipation. In summary, this study leads us to propose that lactulose may improve constipation through a prebiotic effect on gut microbiota and intestinal metabolites.
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FOOD AND AGRICULTURAL IMMUNOLOGY
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2021
·
IF 3.101
Jie-Biao Guo,Tai-Long Wei,Qing-Hua He,Jin-Sheng Cheng,Xiu-Zhen Qiu,Wang-Pei Liu,Xian-Quan Lan,Lu-Fen Chen,Min Guo
A magnetic separation-based immunosensor for deoxynivalenol assay, coupled with a nano-affinity cleaning up for instrumental confirmation, was developed. Maltose-binding protein with mimic epitope of DON was coupled to CdSe/ZnS quantum dot nanobeads for fluorescent reporter. Graphene oxide was linked with magnetic nanoparticles and protein G for immunomagnetic adsorbent. The magnetic separation of reporter could be inhibited by DON so the fluorescent remained in the solution indicates a good quantitative correlation to DON concentration. The DON separated by immune adsorbent can be cleaned up for instrumental confirmation. The optimized immunosensor showed results as follows: the linear range was in 0.3–3000 ng mL−1. The limits of detection (S/N = 3) were 0.50, 1.0, and 3.0 ng mL−1 in different backgrounds. The recoveries for DON-spiked samples range from 78.7% to 88.5%. Both results of UPLC-MS/MS based on affinity column purification and coupled with nano-affinity cleaning up showed a good agreement with the current immunosensor.
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CARBOHYDRATE POLYMERS
·
2020
·
IF 7.182
Xiaoyu Zhang,Huabing Yang,Junping Zheng,Nan Jiang,Guangjun Sun,Xinkun Bao,Aizhen Lin,Hongtao Liu
This study was designed to explore the improvement of chitosan oligosaccharides (COS) on constipation through regulation of gut microbiota. Here, we proved that COS treatment profoundly boosted intestinal motility, restrained inflammatory responses, improved water-electrolyte metabolism and prevented gut barrier damage in constipated mice induced by loperamide . By 16S rDNA gene sequencing, the disbalanced gut microbiota was observed in constipated mice, while COS treatment statistically reversed the abundance changes of several intestinal bacteria at either phylum, family and genus levels, which partly led to the balance in production of intestinal metabolites including bile acids, short-chain fatty acids and tryptophan catabolites. In addition, COS failed to relieve the constipation in mice with intestinal flora depletion, confirming the essentiality of gut microbiota in COS-initiated prevention against constipation. In summary, COS can ameliorate the development of loperamide-induced constipation in mice by remodeling the structure of gut microbial community.
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JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
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2021
·
IF 5.279
Yina Huang,Qin Yang,Xuan Mi,Liang Qiu,Xueying Tao,Zhihong Zhang,Jun Xia,Qinglong Wu,Hua Wei
Ripened Pu-erh tea (RPT) has been shown to be an effective natural ingredient to defend against experimentally induced colitis. We hypothesized that RPT would alleviate dextran sulfate sodium (DSS) induced colitis via modulating intestinal microbiota. The effect of RPT on mice gut microbiota was evaluated using 16S rRNA gene amplicon sequencing, broad-spectrum antibiotic (ABX) treatment, and fecal microbiota transplantation (FMT). Pretreatment with RPT enhanced intestinal barrier function, reduced colonic and serum proinflammatory cytokine and macrophage infiltration, and preserved the resilience of gut microbiota in mice during a DSS challenge. Administration of either RPT-regulated or healthy control-derived gut microbiota showed similar protection against colitis, and such protection could not be recapitulated with fecal microbiota from ABX-treated mice, suggesting a key role of protective consortium in the disease protection. Mechanistically, cecal contents of short-chain fatty acids (SCFAs) and colonic peroxisome proliferator activated receptor-γ (PPAR-γ) expression in colitis mice increased significantly by RPT intervention. Collectively, RPT treatment improved DSS-induced colitis by partially reversing the dysbiosis state of gut microbiota, which might be associated with an increase in SCFA level and PPAR-γ expression.
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Journal of Functional Foods
·
2021
·
IF 4.451
Guoliang Wang,Diying Zhong,Haohan Liu,Tao Yang,Qiqi Liang,Jing Wang,Runguang Zhang,Youlin Zhang
Dietary fiber intake is associated with a low incidence of suffering from metabolic syndrome. To explore the potential health benefits of walnut meal dietary fiber (WMDF) as a prebiotic, the functional role of WMDF on metabolic syndrome in mice induced by high fructose diet (20%, HF) was investigated. The animal experiment results showed that administration of WMDF to HF-fed mice alleviated abnormal body weight gain, insulin resistance, oxidative stress, lipid metabolism disorders and inflammation. Histopathological observation confirmed the preventative effects of WMDF on hepatic steatosis and vascular endothelial dysfunction. Also, WMDF intake increased the production of acetic acid, propionic acid and butyric acid. Moreover, WMDF ingestion effectively improved the disorder of gut microbiota caused by HF, increased the diversity of gut microbiota and the relative abundance of short-chain fatty acids-producing bacteria. These findings demonstrate WMDF can be used as a prebiotic to prevent HF-induced metabolic syndrome.
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