MICROCHEMICAL JOURNAL
·
2020
·
IF 3.594
Lizhen Liu,Zhi Mi,Ziying Guo,Junling Wang,Feng Feng
In this research, a novel label-free fluorescent sensor for the determination of myricetin was designed based on carbon quantum dots (CQDs). The CQDs were prepared via green and straightforward microwave-assisted heating method using aspartic acid and urea as precursors. The prepared CQDs showed strong fluorescence and good solubility in water. The sensing principle of sensor is the quenching of CQDs fluorescence by myricetin via inner filter effect (IFE). The developed CQDs sensor was sensitive to myricetin with a wide linear detection range of 1 to 80 µM and a low limit of detection (LOD) of 18.4 nM. The potential interfering substances showed no influence on the determination of myricetin. Furthermore, the proposed sensor based on CQDs was successfully applied for the determination of myricetin in red wine samples and exhibited simple operation, low cost and high accuracy. The results indicated our developed sensor based on CQDs has great application prospects for myricetin detection in real sample analysis.
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高影响因子
LUMINESCENCE
·
2020
·Vol. 35
·期 8
·1416-1423
·
IF 1.855
Yunyun Sun,Wei He,Xiangying Sun,Bin Liu
A selective and sensitive method for detecting rutin using MoS 2 quantum dots (QDs) as a fluorescent probe is reported in the paper, a one-step hydrothermal synthesis method was used to prepare MoS 2 QDs. The synthesized MoS 2 QDs had good stability and water solubility, and the fluorescence of MoS 2 QDs was quenched by rutin due to the inner filter effect. There was a good linear relationship between the intensity of MoS 2 QDs and rutin concentration ranging from 5.0 × 10 −7 to 3.2 × 10 −5 mol/L with a detection limit of 3.5 × 10 −7 mol/L. MoS 2 QDs showed temperature-dependent fluorescence that had an excellent linear relationship between 20 and 80°C. Therefore MoS 2 QDs can be used as specific fluorescence sensors for selection of rutin and for temperature sensing.
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高影响因子
JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE
·
2020
·Vol. 100
·期 11
·4272-4281
·
IF 2.614
Hanbo Wang,Xiaohong Kou,Caie Wu,Gongjian Fan,Tingting Li
BACKGROUND The effects of postharvest methyl jasmonate (MeJA) treatment (50 μmol L –1 ) on the control of gray mold caused by Botrytis cinerea in blueberry fruit were evaluated by analyzing (i) the levels of disease resistance signals; (ii) the activity of enzymes involved in antioxidant system, disease resistance and phenylpropanoid pathway, and (iii) the secondary metabolite content. RESULTS The results indicated that MeJA treatment significantly restrained the development of gray mold decay in blueberries. The treatment induced a nitric oxide (NO) burst and increased the endogenous hydrogen peroxide (H 2 O 2 ) content in the earlier period of storage. The enhanced NO and H 2 O 2 generation by MeJA treatment might serve as a signal to induce resistance against B. cinerea infection. Furthermore, in inoculated fruit, MeJA treatment significantly promoted antioxidant enzymes and defense-related enzyme activity, which included superoxide dismutase, catalase, ascorbate peroxidase, chitinase, and β -1,3-glucanase, and the degree of membrane lipid peroxidation was reduced. The MeJA treatment enhanced the phenylpropanoid pathway by provoking phenylalanine ammonialyase, cinnamate 4-hydroxylase, and 4-coumarate CoA ligase activity, which was accompanied by elevated levels of phenolics and flavonoids in blueberry fruit. CONCLUSION These results suggested that MeJA could induce the disease resistance of blueberries against B. cinerea by regulating the antioxidant enzymes, defense-related enzymes, and the phenylpropanoid pathway through the activation of signaling molecules.
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高影响因子
JOURNAL OF SEPARATION SCIENCE
·
2020
·Vol. 44
·期 12
·2465-2473
·
IF 2.878
Ting Pan,Yali Lin,Quanzhou Wu,Kaiwen Huang,Jianfeng He
A facile strategy based on the boronate affinity and polydopamine coating was proposed for the preparation of surface molecularly imprinted polymer microspheres using naringin as the glycoside template. The poly(methacrylic acid-co-methyl methacrylate-co-ethyleneglycol dimethacrylate) microspheres were firstly synthesized as inner cores by suspension polymerization method, and then functionalized with 3-aminophenylboronic acid. The imprinted shell layer was obtained by self-polymerization of dopamine on the surface of boronic acid-functionalized polymer microspheres after reversible immobilization of naringin. The resultant surface molecularly imprinted microspheres showed good imprinting efficiency and recognition specificity toward the template molecule in aqueous environment. The isothermal and kinetic adsorption behaviors of the polymers were investigated. The results showed that the covalent surface imprinted microspheres possessed homogeneous recognition sites, strong adsorption affinity, and rapid rebinding kinetics. In addition, the surface imprinted microspheres were utilized as the sorbents of solid phase extraction to successfully separate and enrich naringin from Citri Grandis extract, and the recovery of naringin in eluting solution reached 84.4%.
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高影响因子
ANALYST
·
2020
·Vol. 145
·期 14
·4806-4814
·
IF 3.978
Yi Xiao,Suhua Chen,Guocan Zhang,Zhimao Li,Han Xiao,Chuanpin Chen,Chunlian He,Ran Zhang,Xiaoping Yang
Nicotine analysis is essential to medicine, toxicology and the tobacco industry. However, no simple, portable and disposable method was developed to meet their demands. Here, we report a simple, rapid and disposable silica nanochannel (SAN)-based electrochemiluminescence (ECL) sensor for nicotine analysis by simply assembling a SAN electrode with a paper cover. The sensing principle of the disposable sensor is based on the size exclusion effect and charge selectivity, which obviously prolong the sensor service time. We find that the sensor exhibits good specificity to nicotine, and most of the complex matrices are unlikely to impact the detection. The performance of the disposable sensor in cigarettes, e-cigarettes, nicotine gums, and lozenges is fully validated, showing satisfactory linearity, sensitivity (a limit of detection of 27.82 nM), and accuracy (a recovery between 96.00% and 106.51%). The disposable sensor can be potentially applied for on-site nicotine analysis.
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JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
·
2020
·
IF 3.209
Haifeng Li,Shuang Wang,Fan Cui,Beibei Zhuo,Shuang Wang,Chenrui Zhao,Weilu Liu
Puerarin, an important isoflavone, has been widely used for the treatment of angina and hypertension. In this work, we developed a novel electrochemical sensor for the detection of puerarin based on the hybrid of reduced graphene oxide (RGO) and molecularly imprinted polymer (MIP). The RGO/MIP sensor functions by target puerarin recognition and electro-oxidization via a two-proton and two-electron process, enabling the detection of puerarin with good selectivity and high sensitivity. The MIP layer was integrated on the surface of RGO by the electro-co-polymerization of o-phenylenediamine (monomer) and puerarin (template), resulting in high surface area, binding capacity, good conductivity and faster mass transfer. The nanostructure of the RGO/MIP hybrid was demonstrated using scanning electron microscopy (SEM) and atomic force microscopy (AFM). Experimental conditions involved in the sensor fabrication process were evaluated. Under the optimized condition, a wide linear range (0.02 μM ∼ 40 μM) and a low detection limit (0.006 μM) were achieved. The sensor was applied to detect puerarin in human urine and injection samples, and the result was comparable with that of the gold standard method of high-performance liquid chromatography (HPLC), indicating a promise in the further application to pharmacokinetics or therapeutic drug monitoring.
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SENSORS AND ACTUATORS B-CHEMICAL
·
2020
·
IF 7.1
Yixia Lu,Jian Hu,Yanbo Zeng,Ying Zhu,Hailong Wang,Xiaoling Lei,Shisi Huang,Longhua Guo,Lei Li
A novel electrochemical sensor for rutin determination based on molecularly imprinted poly (ionic liquid) (MIPIL)/ionic liquid-graphene (IL-GR) modified electrode was developed. MIPIL was synthesized via free radical polymerization using rutin as the template, 1-allyl-3-ethyl imidazolium bromide ([AEIm]Br) as the functional monomer and 1,4-butanediyl-3,3′-bis- l -vinylimidazolium dibromide ([V 2 C 4 (mim) 2 ]Br 2 ) as the cross-linker. The composite of IL-GR as an electrode sensitive element was prepared using carboxymethyl-3-methylimidazolium chloride ([HO 2 CMMIm]Cl) by one-step ultrasound method. Fourier transform infrared spectroscopy and scanning electron microscope were used to characterize IL-GR and MIPIL. The increased surface area and conductivity of IL-GR/GCE improved the sensitivity of rutin sensor. Under the optimum condition, good linearity for rutin analysis by the MIPIL-based sensor was obtained from 0.03 to 1 μM. A low limit of detection for rutin was 0.01 μM (S/N = 3). The MIPIL-based sensor demonstrated superiority on DPV response compared to the imprinted sensors based on traditional cross-linkers. The proposed sensor presented good selectivity for rutin and successfully applied for rutin determination in tablets with RSD of 3.05 % and the average recovery range was 98.3 %.
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EUROPEAN FOOD RESEARCH AND TECHNOLOGY
·
2020
·Vol. 246
·期 10
·1909-1918
·
IF 2.366
Zhang Xiao-Hua,Ma Yi-Xin,Yi Chong,Qing Xiang-Dong,Liu Zhi,Zheng Jing-Jing,Lin Fang,Lv Tian-Feng
In this paper, the application of chemometrics coupled with high-performance liquid chromatography-diode array detection (HPLC–DAD) for the rapid and sensitive quantitative analysis of seven flavonoids in the raw and purified propolis samples was investigated. The objective of this paper was to receive a successful resolution of the complex propolis matrix with minimum experimental time (3.5 min) when appearing unknown interferences, highly overlapping peaks, time and baseline drifts among chromatograms. First, the validation samples contained seven flavonoids at five levels, helped to understand and interpret the features of the second-order calibration method based on the alternating trilinear decomposition (ATLD) algorithm. Second, the experimental data were studied for the simultaneous determination of flavonoids in the raw and purified propolis samples using the function of "mathematical separation" of ATLD algorithm. Except for myricetin (MY), the average recoveries of other analytes were 95.79–105.82% for the raw propolis samples, 94.41–108.06% for the purified propolis samples. In addition, the limit of detection (LOD, 0.01–0.20 μg mL −1 ), limit of quantification (LOQ, 0.03–0.60 μg mL −1 ), sensitivity (SEN, 15.97–184.31 mL μg −1 ), selectivity (SEL, 0.16–0.76) and root-mean-squared error of prediction (RMSEP, 0.29–1.64 μg mL −1 ) of the proposed method, demonstrated that all the target analytes have good sensitivity, low detection limit. According to the experimental results, the total content of flavonoids in purified propolis is about twice that in raw propolis, which is consistent with the actual situation. This analytical strategy will be extremely promising for the identification and quantification of analytes studied in the complex samples.
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JOURNAL OF FOOD PROCESSING AND PRESERVATION
·
2020
·Vol. 44
·期 9
·e14691
·
IF 1.405
Liyang Wei,Changhong Liu,Jinjin Wang,Shoaib Younas,Huanhuan Zheng,Lei Zheng
In the current study, fresh-cut broccoli was soaked in melatonin solution before cold storage (4°C, 95% RH) for 25 days. The results indicated that 100 µM melatonin treatment inhibited the increase of L * and b * values and retained higher hue angle, total chlorophyll content, and fresh weight during storage. Furthermore, 100 µM melatonin treatment obviously increased antioxidant contents including ascorbic acid, total phenols, and flavonoids, and enhanced the antioxidant activity of florets. Individual phenolic compounds including epicatechin, rutin, and quercetin were positively influenced by melatonin treatment. Superoxide dismutase (SOD), ascorbate peroxidase (APX), and catalase (CAT) activities were obviously enhanced by 100 µM melatonin treatment whereas peroxidase (POD) activity was significantly reduced. Moreover, florets treated with melatonin exhibited lower malondialdehyde (MDA), H 2 O 2 , and O 2 − levels. Our research concluded that melatonin treatment could be an effective technique for enhancing antioxidant capacity and improving the quality of fresh-cut broccoli during cold storage. Practical applications Fresh-cut fruits and vegetables are more prone to senescence than their intact forms during postharvest storage. Broccoli is a healthy vegetable and has high demands in many countries. In order to reduce economic losses, it is important to delay the senescence and maintain the quality of fresh-cut broccoli during postharvest storage. In this article, 100 µM melatonin treatment was found to significantly enhance antioxidant capacity and delay the senescence of fresh-cut broccoli florets, which maintained the quality of broccoli during cold storage. We believe that melatonin treatment can be applied for the preservation of more fresh-cut fruits and vegetables.
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FOOD AND CHEMICAL TOXICOLOGY
·
2021
·
IF 6.025
Yajing Fang,Fuqiang Liang,Mengmeng Xia,Weiwei Cao,Siyi Pan,Ting Wu,Xiaoyun Xu
This study was aimed to investigate the inhibitory activity of flavonoids on P-glycoprotein (P-gp). Effects of 39 flavonoids on the cellular uptake (CU) of rhodamine123 (Rho) and daunomycin (DNR) were investigated in both parental KB and P-gp overexpressed KB/MDR cells. The inhibition mechanism of selected flavonoids was further investigated by measuring the ATPase activity and expression level of P-gp. Twelve flavonoids improved the uptake of Rho (↑Rho F ) and nineteen flavonoids increased the uptake of DNR (↑DNR F ) in KB/MDR cells with nine flavonoids overlapped. Structure-activity relationship (SAR) indicated that 8-OCH 3 , and 2′-OH have a negative effect on Rho and DNR transport. Whereas 5-OH, 5-OCH 3 , 6-OH, 7-OCH 3 , 3′-OH, and 4′-OH, are essential for inhibition of flavonoids on P-gp and reversing the resistance of Rho and DNR. Eleven selected flavonoids significantly induced the basal P-gp-ATPase activity but much lower than that induced by verapamil . Tangeretin , galangin , kaempferol , quercetin , and morin significantly reversed the ATPase activity stimulated by verapamil. Six of eleven flavonoids significantly decreased P-gp expression, whereas three flavonoids slightly increased P-gp expression. These results provide valuable information that flavonoids can effectively reverse multidrug resistance of P-gp-mediated transport of nutraceutical and drugs by co-administration.
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ANALYTICAL BIOCHEMISTRY
·
2021
·
IF 3.365
Xiao Yu,Wanxiang Yu,Xiaohui Zhang,Yongjie Wang,Shumei Wang,Haiyun Zhai
Based on advantages of capillary electrophoresis (CE), a new solid-phase extraction (SPE) coupled with CE has been developed for preconcentration , enrichment and determination of anthraquinones and flavonoids (rutin, emodin , quercetin , 1,8-dihydroxyanthraquinone) in honey. The environmental-friendly chitin activated after an easy processing is selected as the adsorbent to enrich analytes. Then, chitin was filled into the filter as the solid phase. To improve the extraction effect, some key parameters of extraction were optimized. Under the optional extraction conditions, the chitin showed excellent adsorption capacity and selectivity over rutin , emodin, quercetin, and 1,8-dihydroxyanthraquinone, with enrichment factors reaching 5 folds. The CE coupled with fluorescence detection was used for the detection. Results prove the method is simple, fast, and highly sensitive, with the limit of detection (LOD) is 3.00–200.0 ng/mL; the recovery is 90.0–107.0%, and relative standard deviation of (RSD) is 1.8–8.3%.
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高影响因子
Applied Sciences-Basel
·
2021
·Vol. 11
·期 18
·8296
·
IF 2.679
Jing Wang,Jingluan Wang,Chongde Wu,Jun Huang,Rongqing Zhou,Yao Jin
Greengage wine is gaining increasing attention in Asia for its rich nutritional elements and medicinal value. However, the treatment of the fermentation waste after brewing is a problem that remains unsolved. This work proposed to valorize the fermentation waste extract by a simple centrifugation. The bioactive compounds of the fermentation waste were investigated, including total flavonoids, total phenols, specific phenols and volatile compounds. The antioxidant and antibacterial capacities of the waste extract were also evaluated. The results revealed that the total phenol (1.34 mg GAE/g EPW) and total flavonoid (1.17 mg RE/g EPW) of the fermentation waste extract were still considerable. The fermentation waste also showed high DPPH radical scavenging capacity (5.39 μmol TE/g EPW) and high ABTS radical scavenging capacity (9.80 μmol TE/g EPW). Both GC-MS and LC-MS analysis identified key bioactive compounds, such as linalool, terpineol, β-ionone, neochlorogenic acid and chlorogenic acid, which have high antioxidant capacity and strong, thermal-stable antibacterial capacity. All these characteristics show a promising future for valorized fermentation waste, for example, in food additives or mouthwash.Keywords:greengage;fermentation waste;antioxidant;antibacterial;bioactive compounds
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危险品化学品经营许可证（带存储）