INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2023
·
IF 8.2
Xiaogang Wang,Yu Bai,Qiang He,Jianye Li,Sun Wang,Wei Guo,Xiaozheng Sun
A novel fluorescent probe , fluorescent dialdehyde cellulose (FDAC), was prepared to detect p -phenylenediamine (PPD) in water samples conveniently and quickly. This was achieved by grafting 7-amino-4-methylcoumarin (AMC) onto dialdehyde cellulose (DAC) via an aldol–amine condensation reaction. This method is greener, more economical, and simpler than existing methods for preparing fluorescent probes. The probe was found to be more effective for PPD detection in polar solvents, with less interference from pH and other compounds present in the sample matrix. The photoluminescence of FDAC at λ ex /λ em = 340/430 nm was statically quenched by PPD, allowing for accurate detection within the range of 10–100 μmol/L under optimal conditions, with a detection limit of 3.2 μmol/L (3 σ/s). Meanwhile, the Schiff base (–C=N– group) generated by the condensation of DAC and AMC increased the reaction activity of the fluorescent moiety and changed the AMC conjugated structure, making FDAC more susceptible to aminolysis with PPD than AMC. This study presents a promising solution for fluorescence detection of aniline compounds, with significant potential for application in fields such as environmental analysis.
研究领域:
分析化学
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环境科学
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生物医学
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DOI
PubMed
高影响因子
JOURNAL OF ENVIRONMENTAL MANAGEMENT
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2020
·
IF 5.647
Lei Jia,Wei Liu,Jilin Cao,Zhaoliang Wu,Chunyan Yang
In this study, multi-walled carbon nanotubes (MWCNTs) had been used to strengthen the removal of acid orange 7 (AO7) from the dyestuff wastewater by using foam fractionation. First, the surface modification of MWCNTs was performed by introducing hypochlorite groups (-OCl). The modified MWCNTs were characterized by using SEM , XRD , FTIR and Raman spectroscopy . Subsequently, the potential of modified MWCNTs as a novel collector for AO7 adsorption was examined. The adsorption conditions of modified MWCNTs towards AO7 were optimized by using response surface methodology (RSM) with a central composite design (CCD). The adsorption capacity of modified MWCNTs towards AO7 could reach 47.72 ± 0.79 mg·g −1 under the optimum conditions. The kinetics and the equilibrium adsorption data were analyzed by using different kinetic and isotherm models. According to the regression results, adsorption kinetics data were well described by pseudo-second order model, whereas adsorption isotherm data were best represented by Langmuir isotherm model. Finally, foam fractionation was performed with a batch mode. Under the suitable conditions of loading liquid volume 300 mL, modified MWCNTs dosage 180 mg, cetyltrimethylammonium bromide (CTAB) concentration 50 mg·L −1 , AO7 concentration 30 mg·L −1 , pore diameter of gas distributor 0.125 mm and air flow rate 100 mL·min −1 , the removal percentage and enrichment ratio of AO7 were 91.23% and 6.17, respectively. The decolourization ratio of solution after foam fractionation was found to be 98.66%.
研究领域:
环境科学
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材料科学
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产品关联
DOI
PubMed
高影响因子
DRUG DEVELOPMENT AND INDUSTRIAL PHARMACY
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2019
·
IF 2.367
Xin-Cheng Zhong,Wen-Hong Xu,Zi-Ting Wang,Wang-Wei Guo,Jie-Jian Chen,Ning-Ning Guo,Tian-Tian Wang,Meng-Ting Lin,Zhen-Tao Zhang,Yi-Ying Lu,Qi-Yao Yang,Min Han,Dong-Hang Xu,Jian-Qing Gao
Objective: This study was aimed to develop DOX-TPP loaded acetal-PEG-PCCL micelles to improve the clinical efficacy of drug resistance tumor.Significance: Chemotherapy is one of the main treatments for breast cancer but is plagued by multidrug resistance (MDR). DOX-TPP-loaded micelles can enhance the specific concentration of drugs in the tumor and improve the efficacy and overcome MDR.Methods: In this study, DOX-TPP-loaded micelles based on acetal-PEG-PCCL were prepared and their physicochemical properties were characterized. The cellular uptake and ability to induce apoptosis of the micelles was confirmed by flow cytometry in MCF-7/ADR cells. In addition, cytotoxicity of the micelles was studied in MCF-7 cells and MCF-7/ADR cells. Confocal is used to study the subcellular distribution of DOX. Free DOX-TPP or DOX-TPP-loaded acetal-PEG-PCCL micelles were administered via intravenous injection in the tail vain for the biodistribution study in vivo.Results: The diameter of micelles was about 102.4 nm and their drug-loading efficiency is 61.8%. The structural characterization was confirmed by 1H NMR. The micelles exhibited better antitumor efficacy compared to free doxorubicin in MCF-7/ADR cells by MTT assay. The apoptotic rate and the cellular uptake of micelles were significantly higher than free DOX and DOX-TPP. Micelles can efficiently deliver mitochondria-targeting DOX-TPP to tumor cells. The result of bio-distribution showed that the micelles had stronger tumor infiltration ability than free drugs.Conclusions: In this study, mitochondriotropic DOX-TPP was conjugated to the nanocarrier acetal-PEG-PCCL via ionic interaction to form a polymer, which spontaneously formed spherical micelles. The cytotoxicity and cellular uptake of the micelles are superior to free DOX and exhibit mitochondrial targeting and passive tumor targeting, indicating that they have potential prospects.
研究领域:
药学
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肿瘤学
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纳米技术
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药物递送系统
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产品关联
DOI
PubMed
高影响因子
SEPARATION AND PURIFICATION TECHNOLOGY
·
2021
·
IF 7.312
Yao Zhang,Chao Wu,Huiming Zhu,Baoquan Zhang
Superhydrophobic materials for efficient separation of stabilized oil/water emulsions are in an urgent need to deal with an enormous amount of oily wastewater worldwide. The stainless steel mesh supported zeolitic imidazolate framework-7 (ZIF-7-coated SSM) was fabricated by way of secondary hydrothermal growth, it was further modified by using coordinated polysiloxane elastomer (CPE). Based on characterization by using SEM, XRD, FT-IR and contact angle measurements, the as-synthesized CPE-ZIF-7-coated SSM possessed a rough surface structure of microscale ravines with both superhydrophobicity and superoleophilicity. The stabilities of CPE-ZIF-7-coated SSM were investigated with respect to various treatments including high temperature, corrosion and ultrasonication. The self-healing ability and working mechanism of CPE-ZIF-7-coated SSM were explored by responding to continuous abrasion and O 2 plasma etching. The CPE-ZIF-7-coated SSM exhibited high water-in-oil emulsion separation efficiencies of over 99.9% with a remarkably fast self-healing ability upon damage in less than 10 min without any externally imposed input of matter/energy. The superhydrophobic CPE-ZIF-7-coated SSM with fast self-healing ability would be a promising alternative material for practical applications in separation of emulsive oil/water mixtures.
研究领域:
材料科学
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环境工程
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化学工程
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产品关联
DOI
高影响因子
International Journal of Nanomedicine
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2023
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IF 8
Kai Dong,Zhuang-Zhuang Zhao,Jian Kang,Lei-Ruo Lin,Wen-Ting Chen,Jin-Xi Liu,Xiang-Long Wu,Ting-Li Lu
Background Combined chemotherapy is often affected by the different physicochemical properties of chemotherapeutic drugs, which should be improved by the reasonable design of co-loaded preparations.Purpose A kind of simple but practical graphene oxide (GO) wrapped mesoporous silica nanoparticles (MSN) modified with hyaluronic acid (MSN@GO-HA) were developed for the co-delivery of cinnamaldehyde (CA) and doxorubicin (DOX), in order to enhance their combined treatment on tumor cells and reduce their application defects.Methods The MSNCA@GODOX-HA was constructed by MSNCA (loading CA via physical diffusion) and GODOX-HA (modified with HA and loading DOX via π–π stacking) through the electrostatic adsorption, followed by the physicochemical characterization, serum stability and in vitro release study. Cytotoxicity on different cells was detected, followed by the tumor cell uptake tests. The intracellular reactive oxygen species (ROS) changes, mitochondrial functions and activities of caspase-3/-9 in MCF-7 cells were also evaluated, respectively.Results The MSNCA@GODOX-HA nanoparticles kept stable in FBS solution and achieved pH-responsive release behavior, which was beneficial to increase the accumulation of CA and DOX in tumor cells to enhance the treatment. MSNCA@GODOX-HA exerted higher cytotoxicity to MCF-7 human breast cancer cells than H9c2 cardiac myocyte cells, which were not only attributed to the active targeting to tumor cells by HA, but also related with the activation of intrinsic apoptotic pathway in MCF-7 cells induced by CA, which was mediated by the specific ROS signal amplification and the interference with mitochondrial function. Moreover, the efficacy of DOX was also enhanced by the above process.Conclusion The establishment of the MSNCA@GODOX-HA nanoparticles played a role in promoting strengths and restricting shortcomings of CA and DOX, thereby exerting their function and achieving efficient treatment against cancer.
研究领域:
纳米技术
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肿瘤学
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药学
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材料科学
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产品关联
DOI
PubMed
高影响因子
Analytical Methods
·
2018
·Vol. 10
·期 40
·4894-4901
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IF 2.073
Ya-Ting Zhao,Li-Qing Yu,Xin Xia,Xin-Yu Yang,Wei Hu,Yun-Kai Lv
The adsorption mechanism and desorption properties of volatile organic compounds (VOCs) in a zeolitic imidazolate framework (ZIF-7) were studied by on-line thermal desorption-gas chromatography (TD-GC), and a high efficiency method for determination of harmful indoor gas levels has been developed. The loading volume, loading flow rate and desorption temperature were optimized. Additionally, ZIF-7 has been proved to be an effective adsorbent and its adsorption performance is superior to that of commercial Tenax TA and activated carbon. It has been proved that ZIF-7 is an ideal adsorbent for VOCs. It was suggested that there are hydrophobic interactions, π–π stacking effects, “gate-opening” effects and molecular size effects between adsorbates and ZIF-7. Therefore, the developed ZIF-7-based TD-GC method offered an excellent performance with a detection limit of 1.22–2.38 ng (S/N = 3), a quantification limit of 4.05–7.94 ng (S/N = 10), and a good linearity (R2 = 0.99), and relative standard deviation values (RSD, n = 5) were found to be in the range of 1.69–7.97%, and tube-to-tube reproducibility for three parallel tubes was 8.01%.
研究领域:
材料科学
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环境科学
✓ 已验证
产品关联
DOI
高影响因子
ENVIRONMENTAL POLLUTION
·
2024
·
IF 7.6
Xuewen Yao,Zhaokang Cheng,Evgenios Agathokleous,Yaqian Wei,Xinyuan Feng,Hanhan Li,Tingting Zhang,Shuting Li,Gaurav Dhawan,Xiao-San Luo
As typical antibiotics, tetracycline (TC) and sulfadiazine (SDZ) enter the human body through the food chain. Therefore, it is necessary to understand their individual and combined toxicity. In this study, the effects of TC, SDZ, and their mixture on cell viability, cell membrane damage, liver cell damage, and oxidative damage were evaluated in in vitro assays with human liver cells Huh-7. The results showed cytotoxicity of TC, SDZ, and their mixture, which induced oxidative stress and caused membrane and cell damage. The effect of antibiotics on Huh-7 cells increased with increasing concentration, except for lactate dehydrogenase (LDH) activity that commonly showed a threshold concentration response and cell viability, which commonly showed a biphasic trend, suggesting the possibility of hormetic responses where proper doses are included. The toxicity of TC was commonly higher than that of SDZ when applied at the same concentration. These findings shed light on the individual and joint effects of these major antibiotics on liver cells, providing a scientific basis for the evaluation of antibiotic toxicity and associated risks.
研究领域:
药理学
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毒理学
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细胞生物学
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环境科学
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产品关联
DOI
PubMed
高影响因子
ACS Omega
·
2023
·
IF 4.1
Yue Zhao,Chaoyuan Tang,Jingyi Huang,Hongyan Zhang,Jingbin Shi,Shujun Xu,Lisha Ma,Chun Peng,Qi Liu,Yang Xiong
In this study, we established a simple and rapid in vitro method for screening multidrug resistance (MDR) reversal agents in traditional Chinese medicines (TCMs), which could better correspond to the MDR reversing effect in vivo. Here, D-luciferin, a substrate for the enzyme firefly luciferase and also a substrate for ATP-binding cassette transporters (ABC transporters), was used as the probe to detect its efflux kinetics caused by ABC transporters. First, we established a stable doxorubicin (DOX)-resistant cell line (MCF-7/DOXFluc) that overexpressed luciferase. Then, some kinds of TCMs were chosen for the MDR reversal agents to measure its effect on inhibiting the D-luciferin outflow from MCF-7/DOXFluc, and the ideal reversal agent with the least D-luciferin efflux from MCF-7/DOXFluc was selected to further investigate its effect combined with DOX on MCF-7/DOXFluc tumor-bearing mice. The results indicated that quercetin (Qu) could remarkably increase the retention of D-luciferin in MCF-7/DOXFlucin vitro and in vivo. Also, the combination of Qu and DOX could exceedingly inhibit the tumor growth, which proved the feasibility of this in vitro screening method. The study proposed a feasible method for mass screening of MDR agents from TCMs in vitro.
研究领域:
药理学
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肿瘤学
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传统医学
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DOI
PubMed
高影响因子
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
·
2025
·
IF 4.6
Pengnan Yao,Nan Ma,Jinming Kong,Gengzhi Sun,Xueji Zhang
MicroRNAs (miRNAs) are a class of endogenous, non-coding, single-stranded RNA molecules that are typically 20–25 nucleotides in length. The expression levels of miRNAs in blood, tissue, and other samples are characteristically altered in several disease states. The detection of miRNAs represents a promising avenue for the early diagnosis of various diseases. Consequently, there is a clear need to enhance the sensitivity of miRNA detection to facilitate more accurate disease detection. A fluorescent biosensor was developed that utilizes supramolecular host-guest interactions for assembly and Mo-based materials to initiate a reversible addition-fragmentation chain transfer polymerization (RAFT). The scheme functions by modifying the miRNA on Fe 3 O 4 -magnetic nanoparticles (Fe 3 O 4 -MNPs), followed by linking the initiator of the subsequent RAFT reaction through supramolecular host-guest interaction. Ultimately, polymerization reactions initiated by Mo compounds result in the formation of polymers with fluorescent monomers on Fe 3 O 4 -MNPs. The sensor is extremely sensitive and the detection limit of this sensor is 0.03 fM. Furthermore, the protocol has been demonstrated to be highly effective in the detection of samples in serum, which also serves to illustrate the potential of this fluorescent biosensor for the detection of other kinds of miRNAs.
研究领域:
生物化学
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分子生物学
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纳米技术
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分析化学
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生物传感器
✓ 已验证
产品关联
DOI
PubMed
高影响因子
FOOD AND CHEMICAL TOXICOLOGY
·
2019
·
IF 3.775
Tingyan Hu,Kegang Linghu,Siqi Huang,Maurizio Battino,Milen I. Georgiev,Gokhan Zengin,Defang Li,Yun Deng,Y.T. Wang,Hui Cao
Significant evidence indicated that flaxseed ( Linum usitatissimum ) possesses various positive health aspects such as reducing the risk of cancer and cardiovascular diseases. The fatty acids are considered to be responsible for these benefits of flaxseed. Herein, the in vitro effects of flaxseed extract on the growth and apoptosis of human breast cancer MCF-7 cells were investigated. The MCF-7 cells treated with flaxseed extract showed a dose-dependent decrease in cell viability. The flaxseed extract induced reactive oxygen species and the flow cytometric analysis demonstrated that flaxseed fatty acids triggered apoptosis of MCF-7 cells, which was also shown by the loss of mitochondrial membrane potential and caspase cascade reaction. Thus, the flaxseed extract regulated the growth of MCF-7 cells and induced apoptosis. Eventually, the flaxseed could be used as a dietary supplement to prevent breast cancer.
研究领域:
肿瘤学
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营养学
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细胞生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Systems Microbiology and Biomanufacturing
·
2023
·
IF 0
Li Chang-Long,Li Meng,Zhang Wei-Guo,Xu Jian-Zhong
Menaquinone-7 (MK-7) has an important role in preventing diseases such as cardiovascular disease and osteoporosis. In this study, a combination strategy of strain improvement and medium optimization is investigated to increase MK-7 production in Bacillus amyloliquefaciens . Conventional breeding method was first used to modify the biosynthetic pathway to construct a MK-7 high-producing strain by atmospheric and room temperature plasma mutagenesis and protoplast fusion. The resulted strain Ba-4 with resistance to sulfaguanidine, 1-hydroxy-2-naphthoic acid, menadione, 2-deoxy- d -glucose and rifampicin as well as sensitive to β-fluoropyruvate produced 73.57 ± 1.61 mg/L of MK-7, which was 1.36 times more than that of the parent strain H.β.D.R.-5 (i.e., 31.12 ± 1.40 mg/L). Subsequently, single-factor optimization and response surface methodology (RSM) were used to optimize the medium components for increasing MK-7 production by strain Ba-4. Strain Ba-4 produced 90.43 ± 1.32 mg/L of MK-7 under the single-factor optimized medium. Moreover, the results of response surface methodology indicated that glycerol, soy peptone and Tween-80 had significant effects on MK-7 production, and the highest MK-7 production (i.e., 95.03 ± 1.01 mg/L) was obtained under the optimized medium, which was 0.29 times higher than that of the initial medium. These results confirmed that the conventional breeding methods and fermenter control system are effective strategies in improving MK-7 production by B. amyloliquefaciens .
研究领域:
微生物学
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生物技术
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发酵工程
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DOI
Journal of Materials Chemistry B
·
2017
·Vol. 5
·期 21
·3970-3981
·
IF 4.543
Mengjun Zhang,Shasha Jing,Jie Zhang,Jiulong Zhang,Xinlong Zang,Mingxi Qiao,Xiuli Zhao,Haiyang Hu,Dawei Chen
Multidrug resistance (MDR) has been a major obstacle to tumor chemotherapy. Pluronic unimers have been reported to be promising copolymers to reverse MDR, and the intracellular delivery of Pluronic unimers is a problem worth thinking. To exert the excellent reversal effect of Pluronic unimers, DOX-loaded G4.0 PAMAM was modified with PluronicL64 via cis-aconitic acid as a pH-sensitive linkage (PCPAMAM/DOX), which could release DOX and Pluronic unimers into cytoplasm. The Pluronic-modified PAMAM (PCPAMAM) exhibited favorable biocompatibility and pH-sensitivity. PCPAMAM/DOX showed a nano-scale size and a sustained in vitro release profile. Compared with a control formulation, PCPAMAM/DOX showed a higher reversal effect on MCF-7/ADR cells and enhanced intracellular drug accumulation. The results of P-gp activity, subcellular distribution of PluronicL64, the ATP level and mitochondrial transmembrane potential all illustrated that free Pluronic unimers could be released by PCPAMAM functioning as reversal agents. In conclusion, PCPAMAM could be a promising vehicle to enhance DOX accumulation by overcoming MDR in MCF-7/ADR cells. This work also provided an effective method to deliver Pluronic unimers into MDR cells.
研究领域:
药剂学
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癌症研究
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纳米技术
·
药物递送系统
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）