CHEMICAL ENGINEERING JOURNAL
·
2023
·
IF 15.1
Decai Zeng,Kai Zhang,Haoran Huang,Xiaohua Zhang,Zongde Wang,Shangxing Chen,Guangbin Liu,Yangping Wen,Peng Wang
A general strategy for the in-situ hydrothermal synthesis of transition metal sulfides functionalized hierarchically porous carbons (FeS 2 -HPC, Co 9 S 8 -HPC, Ni 3 S 2 -HPC, Cu 2 S-HPC, and ZnS-HPC) is proposed using different transition metal chlorides as metal sources and activators, sustainable agroforestry waste biomass ( Citrus sinensis peel sponge layer) as a carbon source, sulfuric acid as a sulfur source, and silica colloid as a hard template. The introduction of both transition metal salts and silica colloids facilitate the adjustment of the pore structure. The added sulfuric acid promotes the degradation of the agroforestry waste biomass, which are conducive to the formation of porous carbons. The in-situ synthesis of transition metal sulfide particles and the sulfur doping improve the conductivity, electrocatalytic and enzyme-like activities of obtained composite materials. The FeS 2 -HPC with multiple enzyme-like activities and the best electrocatalytic activity is applied as a nanozyme electrochemical sensing platform for the simultaneous detection of three ATP metabolites with a limit of detection (LOD) of 0.069 μM for uric acid in a linear range of 0.3–200 μM, a LOD of 0.047 μM for xanthine in 0.3–100 μM, and a LOD of 0.21 μM for hypoxanthine in 0.7–300 μM. This work provides a new idea for the development of transition metal sulfides functionalized porous carbons from agroforestry waste biomass, and a theoretical basis for the fabrication of nanozyme electrochemical sensing platform for health assessment and disease prevention.
研究领域:
材料科学
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电化学
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纳米技术
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分析化学
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生物材料
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DOI
高影响因子
MICROCHEMICAL JOURNAL
·
2024
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IF 4.9
Yuxiang Xia,Hongyu Li,Yating Lu,Yanfei Zhang,Kaixin Xue,Jiaheng Zhang,Yujie Zhao,Xingyu Luo
Adenosine deaminase (ADA) is a vital enzyme for regulating the biological process in humans and its abnormal expression level is related to various severe diseases. Nevertheless, conventional approaches for detecting ADA activity are hindered by the drawbacks of complexity and limited detection performances. To address these challenges, we here designed an ADA-responsive aptasensing strategy based on a synthesized benzothiazole-coumarin G-quadruplex (G4) fluorescent probe, which can bind to the G-rich ATP aptamer (G-APA) and form the high-contrast fluorescent complex (S1/G-APA). While the fluorescence of S1/G-APA could be quenched by ATP competition due to the allosteric process, the deamination in ATP catalyzed by ADA would recover the fluorescence of S1/G-APA and allow a facile, turn-on, label-free, and selective detection of ADA activity. This proposed method exhibits favorable detection performances for ADA activity analysis with a wide detection linear range varied from 1 to 100 U/L and a detection limit of 0.82 U/L. Meanwhile, it can be applied for ADA inhibitor (DCF) evaluation and provide a turn-off and sensitive detection performances. Inspired by these detection efficacies, the serum samples spiked with ADA and DCF have been investigated to present the satisfied recovery rates and detection reliability. Collectively, this method will provide a promising way for portable and efficient ADA activity quantification and evaluating the inhibition efficiencies of ADA inhibitors, which hold the potential in promoting ADA-related sensing and pharmaceutical research.
研究领域:
生物化学
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分析化学
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分子生物学
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药学
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DOI
高影响因子
BIOSENSORS & BIOELECTRONICS
·
2022
·
IF 12.545
Xiaojuan Wang,Zefeng Mao,Ruipeng Chen,Shuning Li,Shuyue Ren,Jun Liang,Zhixian Gao
DNA origami technology has great potential for biosensor applications. Here, we described the construction of a self-assembled DNA origami biosensor for the precise localization of fluorescent aptamers . Due to the molecular weight difference between DNA origami and aptamer, centrifugal filters were used to quantitatively detect adenosine triphosphate (ATP). The ATP-specific aptamer labeled with fluorescence reporter 6-carboxyfluorescein FAM (FAM-aptamer) was selected as the recognition element and signal probe . ATP duplexed aptamers bound to triangular DNA origami by base-complementary pairing, resulting in high fluorescence signals on the origami arrays. The competitive binding of ATP toward the FAM-aptamer triggered the release of FAM-aptamer-ATP complexes from the surface of the origami array, resulting in weakened fluorescence signals. For ATP quantification, 100 kD centrifugal filters were employed, followed by measurement of the fluorescence signal trapped on the origami arrays of the filter device. The successful synthesis of origami-aptamer arrays was characterized by atomic force microscopy , laser confocal microscopy , and electrophoresis . Fluorescence measurements exhibited an excellent linear relationship with logarithms of ATP concentrations within 0.1–100 ng mL −1 , with a detection limit of 0.29 ng mL −1 . By replacing aptamers and complementary strands, we demonstrated the potential of this method for 17β-estradiol detection. Considering that the detection mechanism is based on the hybridization and displacement of DNA strands, the detection system had the potential for recharging. Our study provides new insights into applying DNA origami technology in small molecule detection.
研究领域:
生物传感器
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纳米技术
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分子生物学
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分析化学
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DOI
PubMed
高影响因子
JOURNAL OF MATERIALS SCIENCE & TECHNOLOGY
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2024
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IF 11.2
Rui Zhang,Zhiling Chen,Yi Li,Delun Chen,Tao Wang,Bingrong Wang,Qionglin Zhou,Shaowen Cheng,Dan Xu,Xiaohong Wang,Lina Niu,Jinchun Tu,Qiang Wu
The use of zinc oxide (ZnO) photosensitizers (PSs)-mediated photodynamic therapy (PDT) against bacterial wound infections is greatly restricted by diminished photocatalytic efficiency caused by the rapid recombination of photogenerated electrons and holes. In this work, ZnO PSs with a Ni-doped/oxygen vacancy and a protective carbon shell were successfully synthesized by calcinating a Ni-doping zeolitic imidazolate framework-8 precursor. The double-defect structure and the carbon-based substrates significantly promoted the efficiency of photogenerated electron–hole pair separation, meanwhile, the optimal proportion of 3% Ni doping endows it with the best photocatalytic performance as elucidated by photodegradation assays of methylene blue (MB) and density functional theory (DFT) calculations, reinforcing the generation of reactive oxygen species (ROS) by ZnO and showing obvious advantages in antibacterial properties. As the ROS damage that facilitates the photogenerated electron transfer underwent localized accumulation, the PSs disturbed the bacterial membrane integrity and caused bacterial ATP synthesis inhibition, further leading to bacterial lysis and promoting bacterial deaths. Additionally, the PSs showed outstanding efficacy in eradicating bacterial biofilms. Simultaneously, the significantly enhanced PDT antibacterial performance of the PSs in vivo could initiate wound tissue repair and trigger anti-inflammatory reactions by significantly regulating the expression levels of regeneration- and inflammation-related genes or proteins. Furthermore, the PSs consistently exhibited favorable compatibility both in vitro and in vivo . In summary, this study offers evidence of the remarkably efficient and biologically safe performance of Ni-ZnO@C PSs, with antibacterial properties advancing wound healing, both in controlled laboratory environments and living organisms, further underscoring their substantial potential for biomedical applications.
研究领域:
材料科学
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生物医学工程
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光疗
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微生物学
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伤口愈合
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DOI
高影响因子
BIOSENSORS & BIOELECTRONICS
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2014
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IF 6.451
Lu Lu,Jing Cao Si,Zhong Feng Gao,Yu Zhang,Jing Lei Lei,Hong Qun Luo,Nian Bing Li
A dual strategy that combines the adenosine triphosphate (ATP)-dependent enzymatic ligation reaction with self-cleaving DNAzyme-amplified electrochemical detection is employed to construct the biosensor. In this design, the methylene blue-labeled hairpin-structured DNA was self-assembled onto a gold electrode surface to prepare the modified electrode through the interaction of Au–S bond. In the procedure of ATP-dependent ligation reaction, when the specific cofactor ATP was added, the two split oligonucleotide fragments of 8-17 DNAzyme were linked by T4 DNA ligase and then released to hybridize with the labeled hairpin-structured DNA substrate. The linked 8-17 DNAzyme catalyzes the cleavage of the hairpin-structured substrate by the addition of Zn 2+ , causing the methylene blue which contains high electrochemical activity to leave the surface of the gold electrode, therefore generating a dramatic decrease of electrochemical signal. The decrease of peak current was readily measured by square wave voltammetry and a relatively low detection limit (0.05 nM) was obtained with a linear response range from 0.1 to 1000 nM. By taking advantage of the highly specific cofactor dependence of the DNA ligation reaction, the proposed ligation-induced DNAzyme cascades demonstrate ultrahigh selectivity toward the target cofactor ATP. A catalytic and molecular beacons strategy is further adopted to amplify the electrochemical signal detection achieved by cycling and regenerating the 8-17 DNAzyme to realize enzymatic multiple turnover, thus one DNAzyme can catalyze the cleavage of several hairpin-structured substrates, which improves the sensitivity of the newly designed electrochemical sensing system.
研究领域:
生物化学
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电化学
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生物传感器
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纳米技术
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DOI
PubMed
高影响因子
ANALYTICAL CHEMISTRY
·
2023
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IF 7.4
Zhiming Wang,Jing Tan,Mengxuan Li,Can Gao,Wenwen Li,Jing Xu,Changchuan Guo,Zhenzhen Chen,Rong Cai
Adenosine triphosphate (ATP) is the major energy carrier in organisms, and there are many cellular proteins that can bind to ATP. Among these proteins, kinases are key regulators in several cell signaling processes, and aberrant kinase signaling contributes to the development of many human diseases, including cancer. Hence, small-molecule kinase inhibitors have been successfully used for the treatment of various diseases. Since the ATP-binding pockets are similar for many kinases, it is very important to evaluate the selectivity of different kinase inhibitors. We report here a clickable ATP photoaffinity probe for the global profiling of ATP-binding proteins. After incubating the protein lysate with the ATP probe followed by ultraviolet (UV) irradiation, ATP-binding proteins were labeled with an alkyne handle for subsequent biotin conjugation through click chemistry. Labeled proteins were enriched with streptavidin beads, digested with trypsin, and analyzed using liquid chromatography–tandem mass spectrometry (LC-MS/MS). More than 400 ATP-binding proteins, including approximately 200 kinases, could be identified in a single LC-MS/MS run in the data-dependent acquisition mode. We then applied this method to the analysis of targets of three selected ATP-competitive kinase inhibitors. We were able to successfully identify some of their reported target proteins from label-free quantification results and validated the results using Western blot analyses. Together, we developed a clickable ATP photoaffinity probe for proteome-wide profiling of ATP-binding proteins and demonstrated that this chemoproteomic method is amenable to high-throughput target identification of kinase inhibitors.
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PubMed
高影响因子
Journal of Neuroimmune Pharmacology
·
2024
·Vol. 19
·期 1
·1-17
·
IF 5.2
Lin Yun,Liu Tian,Chen Hong,Zeng Ming,Hu Shunwei,Yu Xiaoning,Chen Yonghua,Xia Chunmei,Wang Jin,Wang Jijiang
The occurrence of major asthma symptoms is largely attributed to airway vagal hypertonia, of which the central mechanisms remain unclear. This study tests the hypotheses that endothelin-1-mediated brainstem glial activation produces asthmatic airway vagal hypertonia via enhanced action of adenosine 5’-triphosphate on neuronal purinergic P2X4 receptors. A rat model of asthma was prepared using ovalbumin. Airway vagal tone was evaluated by the recurrent laryngeal discharge and plethysmographic measurement of pulmonary function. The changes in the brainstem were examined using ELISA, Western blot, luciferin-luciferase, quantitative reverse transcription-polymerase chain reaction, enzyme activity assay and immunofluorescent staining, respectively. The results showed that in the medulla of rats, endothelin receptor type B and P2X4 receptors were primarily expressed in astrocytes and neurons, respectively, and both of which, along with endothelin-1 content, were significantly increased after ovalbumin sensitization. Ovalbumin sensitization significantly increased recurrent laryngeal discharge, which was blocked by acute intracisternal injection of P2X4 receptor antagonist 5-BDBD, knockdown of brainstem P2X4 receptors, and chronic intraperitoneal injection of endothelin receptor type B antagonist BQ788, respectively. Ovalbumin sensitization activated microglia and astrocytes and significantly decreased ecto-5’-nucleotidase activity in the medulla, and all of which, together with the increase of medullary P2X4 receptor expression and decrease of pulmonary function, were reversed by chronic BQ788 treatment. These results demonstrated that in rats, allergic airway challenge activates both microglia and astrocytes in the medulla via enhanced endothelin-1/endothelin receptor type B signaling, which subsequently causes airway vagal hypertonia via augmented adenosine 5’-triphosphate/P2X4 receptor signaling in central neurons of airway vagal reflex. Graphical Abstract
研究领域:
神经科学
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药理学
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呼吸医学
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免疫学
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DOI
PubMed
高影响因子
JOURNAL OF COLLOID AND INTERFACE SCIENCE
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2024
·
IF 9.4
Yize Liu,Ning Jiang,Si Zuo,Ying Feng,Ran Chen,Yiwei Zhang,Naiwen Zhang,Xiaoyu Sang,Qijun Chen
Hypothesis Trypanosomiasis is one of the main threats to human and animal health in African countries. Trypanosoma brucei can evade the host immune recognition by rapidly altering its variant surface glycoprotein (VSG). The ATP synthase F 1 subunit of the parasite exhibits extremely low similarity to that of its mammalian hosts, hypothetically making it an ideal target for the development of novel therapeutics. Experiments Graphene quantum dots (GQDs) were synthesized, and their adhesion to T. brucei surface and internalization was observed microscopically. The activity of ATP synthase and mitochondrial membrane potential of T. brucei were measured after exposure to GQDs. Proteomics, biolayer interferometry, and molecular dynamic simulations were utilized to evaluate the interaction between GQDs with the target proteins. Findings GQDs specifically adhered to the VSG of T. brucei and were conveyed inside the parasite via the VSG internalization pathway. The GQDs promoted intracellular ROS production, interacted with, and inhibited the activity of the p18 subunit of ATP synthase, disrupted parasite mitochondrial membrane potential. Additionally, the GQDs caused a decrease in aminoacyl – tRNA biosynthesis, and upregulated RNA and protein degradation pathways. The findings of this study offer a novel avenue for the target-oriented discovery of anti-trypanosome drugs.
研究领域:
寄生虫学
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纳米技术
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分子生物学
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药物发现
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DOI
PubMed
高影响因子
BIOSENSORS & BIOELECTRONICS
·
2015
·
IF 6.409
Ying Zhu,Xiao-chun Hu,Shuo Shi,Ru-ru Gao,Hai-liang Huang,Yan-yan Zhu,Xiao-yan Lv,Tian-ming Yao
We report here an ultrasensitive strategy based on the recognition-induced conformational alteration of aptamer and fluorescence turn-on abilities of guanine-rich (G-rich) DNA sequence in proximity to silver nanoclusters for adenosine triphosphate (ATP), adenosine (A) and thrombin (TB) detection. Herein, we designed two tailored DNA sequences noted as complementary DNA (abbreviated as c-DNA) and signal probe DNA (abbreviated as s-DNA), respectively. c-DNA is designed as a special structure consisting of a sequence complementary to aptamer at the 3′-end and a guanine-rich DNA sequence at the 5′-end; s-DNA contains a cytosine-rich sequence responsible for Ag NCs templated synthesis at the 3′-end and a link sequence (part of aptamer) complementary to partial of the c-DNA at the 5′-end. In the presence of target, the aptamer associated with the target, resulting in the formation of duplex DNA (dsDNA), the DNA–Ag NCs thereafter could close to the guanine-rich sequence, leading to enhanced fluorescence signal readout. The widespread application of the sensing system is achieved success in the detection of three biomolecules . ATP, adenosine and thrombin in the range of 0.5-8.0 μM, 0.5-7.0 μM and 50-900 nM could be linearly detected with the detection limits of 91.6 nM, 103.4 nM and 8.4 nM, respectively. This label-free and turn-on fluorescent sensing system employing the mechanism proposed here turns out to be sensitive, selective, and convenient for the detection of biomolecules without washing and separation steps.
研究领域:
分析化学
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生物传感器
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纳米技术
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生物化学
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DOI
PubMed
高影响因子
NANO
·
2025
·
IF 1.1
Xiaofeng Yang,Dongdong Li,Longgui Peng,Jiushuang Huang
Manganese dioxide (MnO 2 ), known for its high theoretical specific capacitance, is considered one of the most promising electrode materials for supercapacitors. However, its poor intrinsic conductivity and structural degradation during cycling severely limit its practical application. To address these challenges, an interwoven MnO 2 nanofiber network-based attapulgite (ATP)/MnO 2 composite was constructed by in-situ growth of MnO 2 nanofibers on natural ATP clay scaffolds via a one-step hydrothermal method. The microstructures and morphologies of pure ATP, pure MnO 2 , and the ATP/MnO 2 composite were systematically characterized and compared. Furthermore, the electrochemical performances of pure MnO 2 and the ATP/MnO 2 composite were evaluated. Results indicate that the ATP/MnO 2 composite possesses a more favorable specific surface area and pore structure than pure ATP or MnO 2 , with an approximate 40% increase in specific surface area. Electrochemical measurements demonstrated that the ATP/MnO 2 composite delivered a specific capacitance of 218.8 F g -1 at a current density of 0.5 A g -1 , approximately 50% higher than that of pure MnO 2 . Moreover, the composite exhibited outstanding cycling stability, retaining 89.9% of its initial capacitance after 5000 charge-discharge cycles at 3 A g -1 . This study demonstrates the role of natural ATP in synergistically optimizing the specific surface area and active sites of MnO 2 -based materials. The proposed strategy offers a feasible route for designing high-performance, low-cost, and sustainable MnO 2 -based electrodes, while also providing new insights into the development of long-lasting energy storage materials.
研究领域:
材料科学
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储能
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纳米技术
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电化学
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产品关联
DOI
高影响因子
JOURNAL OF MATERIALS RESEARCH
·
2025
·
IF 2.9
Ji Xinyuan,Han Yuliang,Fang Jiahui,Tian Dating,Hu Sheng
In this study, a novel ion-imprinted polymer (IIP) was synthesized using attapulgite (ATP) and chitosan (CS) as the substrates and methyl methacrylate (MMA) as the monomer. The IIP (MMA) was characterized by FTIR, XRD, SEM, TEM, BET, and XPS. Orthogonal experiments were analyzed to show that when initial concentration of Cd 2+ was 100 mg/L, and the adsorption time was 20 min, under the condition of IIP (MMA) and NIP (MMA) on Cd adsorption capacity, were 57.56 mg/g and 52.03 mg/g, at the same time, the adsorption capacity of Se were 17.14 mg/g and 15.86 mg/g, far below the Cd adsorption capacity. The results showed that IIP(MMA) synthesized with ATP and CS as substrates and MMA as functional monomer has been successfully synthesized and can be used for selective adsorption of Cd 2+ in water. Graphical abstract
Illustration of the imprint polymerization process
研究领域:
环境科学
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材料科学
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DOI
高影响因子
CELL PROLIFERATION
·
2019
·Vol. 53
·期 2
·e12737
·
IF 5.039
Xiao-Wei Gu,Yan Yang,Tao Li,Zi-Cong Chen,Tao Fu,Ji-Min Pan,Jian-Ping Ou,Zeng-Ming Yang
Objective Embryo implantation needs a reciprocal interaction between competent embryo and receptive endometrium. Adenosine triphosphate (ATP) produced by stressed or injured cells acts as an important signalling molecule. This study aims to investigate whether adenosine triphosphate (ATP) plays an important role in the dialogue of human blastocyst-endometrium. Materials and methods The concentration of lactate was analysed in culture medium from human embryos collected from in vitro fertilization patients. Extracellular ATP was measured by ATP Bioluminescent Assay Kit. Ishikawa cells and T-HESCs were treated with ATP, ATP receptor antagonist, ATP hydrolysis enzyme or inhibitors of ATP metabolic enzymes. The levels of gene expression were evaluated by real-time PCR and immunoassay. Results We showed that injured human endometrial epithelial cells could rapidly release ATP into the extracellular environment as an important signalling molecule. In addition, blastocyst-derived lactate induces the release of non-lytic ATP from human endometrial receptive epithelial cells via connexins. Extracellular ATP stimulates the secretion of IL8 from epithelial cells to promote the process of in vitro decidualization. Extracellular ATP could also directly promote the decidualization of human endometrial stromal cells via P2Y-purinoceptors. More importantly, the supernatants of injured epithelial cells clearly induce the decidualization of stromal cells in time-dependent manner. Conclusion Our results suggest that ATP should play an important role in human blastocyst-endometrium dialogue for the initiation of decidualization.
研究领域:
生殖生物学
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分子生物学
·
细胞信号传导
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PubMed
高影响因子
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