ACS Applied Materials & Interfaces
·
2012
·
IF 4.525
Bo Yuan,Qiang Chen,Wen-Quan Ding,Ping-Sheng Liu,Shi-Shan Wu,Si-Cong Lin,Jian Shen,Yue Gai
AB diblock copolymers comprised of poly(2-methacryloyloxyethyl phosphorylcholine) (PMPC) and poly(3-methacryloxypropyl trimethoxysilane) (PMTSi) segments, which are used for biocompatible coatings, were investigated. Block copolymers with various compositions were synthesized by atomic transfer radical polymerization (ATRP). The obtained copolymers were dissolved in an ethanol solution, and dynamic light scattering showed that all block copolymers were capable of existing as micelles. After a convenient “one-step” reaction, the cellulose membranes could be covalently modified by these copolymers with stable chemical bonds (C–O–Si and Si–O–Si). Block copolymers with different PMPC chain length were applied to surface modification to find the most suitable copolymer. The functional MPC density can be controlled by adjusting the ratio of the two monomers (MPC and MTSi), which also affect surface properties, including the surface contact angle, surface morphology, and number of functional PC groups. The low-fouling properties were measured by protein adsorption, platelet adhesion and activation, and cell adhesion. Protein adsorption of bovine serum albumin (BSA), fibrinogen, and human plasma were also tested and a moderate monomer composite was attained. The protein adsorption behavior on the novel interfaces depends both on MPC density and PMPC chain length. Platelet adhesion and activation were reduced on all the modified surfaces. The adhesion of Human Embryonic Kidney 293 (293T) cells on the coated surfaces also decreased.
研究领域:
材料科学
·
生物材料
·
生物医学工程
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DOI
PubMed
高影响因子
Frontiers in Bioengineering and Biotechnology
·
2021
·
IF 5.89
Yu Juan,Xu Chaoqun,Lu Chuanwei,Liu Qian,Wang Jifu,Chu Fuxiang
Preparation and application of sustainable polymers derived from renewable resources are of great significance. The aim of this study is to synthesize a kind of sustainable polymeric micelles from rosin and vegetable oils via atom transfer radical polymerization (ATRP) and to investigate the doxorubicin delivery properties of these micelles. Dehydroabietic acid–based poly lauryl methacrylate (DA-PLMA) with narrow PDI of 1.13 was prepared in a well-controlled process using rosin as an ATRP initiator. Thereafter, carboxylic groups were introduced to form poly methacrylic acid (PMAA) moieties in DA-PLMA polymer via acid hydrolysis. The resulted DA-PLMA-PMAA could self-assemble in water to form pH-dependent polymeric micelles with a diameter of ∼65 nm and PDI as low as 0.105. Owing to the existence of rosin, DA-PLMA-PMAA micelles also showed self-fluorescence properties. In addition, Dox-loaded micelles were prepared in aqueous solution with the drug-loading capacity as high as 16.0% and showed sustained-release characteristics. These results demonstrate great promise for designing polymeric micellar from rosin and vegetable oils.
研究领域:
材料科学
·
生物医学工程
·
药剂学
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DOI
高影响因子
CARBOHYDRATE POLYMERS
·
2010
·Vol. 84
·期 1
·195-202
·
IF 3.167
Deqian Wang,Junjun Tan,Hongliang Kang,Lin Ma,Xin Jin,Ruigang Liu,Yong Huang
The pH-responsive ethyl cellulose graft poly(2-(diethylamino) ethyl methacrylate) (EC- g -PDEAEMA) copolymers were synthesized through atom transfer radical polymerization (ATRP). Kinetic analysis confirmed the reaction is living and controllable. The graft copolymers can form micelles in acid aqueous medium. The critical micelle concentration (CMC) of the graft copolymers decreases with the increase of the graft length and graft density. The resultant micelles show the pH-sensitivity. The decrease in the hydrodynamic radius of the micelles at pH 6–6.9 attributes to collapse of the side PDEAEMA chains in the shell of the micelles due to the deprotonation , which was further confirmed by TEM observation. The loading and controlled release of drugs in the micelles was investigated by using rifampicin (RIF) as the model drug. It was found that the cumulant release of RIF in the buffer solution at pH 6.6 is higher than that at pH 7.4.
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DOI
高影响因子
JOURNAL OF SOLID STATE ELECTROCHEMISTRY
·
2018
·Vol. 23
·期 2
·583-590
·
IF 2.509
Sun Yue,Zhao Mengyuan,Liu Yutong,Fu Liye,Li Siyu,Yang Yifei
Erythromycin (ERY) molecular imprinted polymer was prepared on the surface of Au electrode modified with Ni and Au nanoclusters (MIP/AuNCs/Ni/Au) via metal-free visible-light–induced atom transfer radical polymerization, which was very easy, controllable, and environmentally benign. MIP/AuNCs/Ni/Au was examined by energy dispersive spectrometer (EDS), scanning electron microscope (SEM), cyclic voltammetry (CV), and electrochemical impedance spectroscopy (EIS). Further, MIP/AuNCs/Ni/Au was successfully used for the determination of ERY by differential pulse voltammetry (DPV). Under the optimal conditions, MIP/AuNCs/Ni/Au gave a linear correlation over the ERY concentration from 1.0 × 10 −8 to 1.0 × 10 −1 mg/L with the detection limit of 3.2 × 10 −9 mg/L ( S / N = 3). Comparing with the similar sensors, the larger linear range and lower detection limit indicated the promising prospect of this electrochemical sensor. Hence, this study was of great importance for the polymer-modified electrodes and the sensitive detection of ERY.
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DOI
高影响因子
TALANTA
·
2021
·
IF 6.057
Ru Bai,Yue Sun,Mengyuan Zhao,Zhen Han,Juntong Zhang,Yuze Sun,Wenjing Dong,Siyu Li
Immunoglobulin G (IgG) is related to the occurrence of many diseases, such as measles and inflammatory. In this paper, IgG imprinted polymers (IgGIPs) were fabricated on the surface of nano Au/nano Ni modified Au electrode (IgGIPs/AuNCs/NiNCs/Au) via metal-free visible-light-induced atom transfer radical polymerization (MVL ATRP). The IgGIPs were prepared by IgG conjugated with fluorescein isothiocyanate (FITC-IgG) as both a template and a photocatalyst. After the templates were removed, the photocatalysts (FITC) would not remain in the polymer and avoided all the effect of catalysts on the electrode. The fabricated electrodes were examined by cyclic voltammetry (CV), electrochemical impedance spectroscopy (EIS), scanning electron microscope (SEM) and X-ray photoelectron spectroscopy (XPS). Under the optimized conditions, IgGIPs/AuNCs/NiNCs/Au was prepared and used as an electrochemical biosensor. The biosensor could be successfully applied for the determination of IgG by differential pulse voltammetry (DPV) measurement. The results showed that the proposed biosensor displayed a broader linear range and a lower detection limit for IgG determination when it was compared to those similar IgG sensors. The linear range from 1.0 × 10 −6 mg L −1 to 1.0 × 10 1 mg L −1 was obtained with a low detection limit (LOD) of 2.0 × 10 −8 mg L −1 ( S / N = 3). Briefly, the biosensor in this study introduced an easy and non-toxic method for IgG determination and also provided a progressive approach for designing protein imprinted polymers.
研究领域:
分析化学
·
生物传感器
·
聚合物科学
·
电化学
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DOI
PubMed
高影响因子
ANALYTICAL CHEMISTRY
·
2020
·
IF 6.785
Qiong Hu,Shiyu Gan,Yu Bao,Yuwei Zhang,Dongxue Han,Li Niu
As a single-chain glycoprotein with endopeptidase activity, the prostate-specific antigen (PSA) is valuable as an informative serum marker in diagnosing, staging, and prognosis of prostate cancer. In this report, an electrochemical biosensor based on the target-induced cleavage of a specific peptide substrate (PSA peptide) is designed for the highly selective detection of PSA at the femtomolar level, using electrochemically controlled atom transfer radical polymerization (eATRP) as a method for signal amplification. The PSA peptides, without free carboxyl sites, are attached to the gold surface via the N-terminal cysteine residue. The target-induced cleavage of PSA peptides results in the generation of carboxyl sites, to which the alkyl halide initiator α-bromophenylacetic acid (BPAA) is linked via the Zr(IV) linkers. Subsequently, the potentiostatic eATRP of ferrocenylmethyl methacrylate (FcMMA, as the monomer) leads to the surface-initiated grafting of high-density ferrocenyl polymers. As a result, a large amount of Fc redox tags can be recruited for signal amplification, through which the limit of detection (LOD) for PSA can be down to 3.2 fM. As the recognition element, the PSA peptide is easy to synthesize, chemically and thermally stable, and low-cost. Without the necessity of enzyme or nanoparticle labels, the eATRP-based amplification method is easy to operate and low-cost. Results also show that the cleavage-based electrochemical PSA biosensor is highly selective and applicable to PSA detection in complex biological samples. In view of these merits, the integration of the eATRP-based amplification method into cleavage-based recognition is believed to hold great promise for the electrochemical detection of PSA in clinical applications.
研究领域:
生物医学工程
·
分析化学
·
癌症诊断
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产品关联
DOI
PubMed
高影响因子
INDUSTRIAL CROPS AND PRODUCTS
·
2021
·
IF 5.645
Juan Yu,Chaoqun Xu,Xiaoli Song,Chuanwei Lu,Chunpeng Wang,Jifu Wang,Fuxiang Chu
Rosin is an important renewable resource. However, preparation of rosin-based polymers with good performance is still challenging. In this work, a series of rosin-based polymers were prepared via “grafting from” atom transfer radical polymerization (ATRP) using dehydroabietic acid (DA, extracted from pine trees) derived ATRP initiator (2-BriBEDA, (2-bromoisobutyric) hydroxyethyl ester of dehydroabietic acid). The graft polymerization behavior of polar monomer methyl methacrylate (MMA), nonpolar monomer lauryl methacrylate (LMA) from DA were investigated. The kinetic studies showed that the ATRP processes of MMA, LMA as well as MMA- co -LMA were all living and controlled, which indicated that 2-BriBEDA was a good ATRP initiator. With the introduction of DA, all rosin-based copolymers showed improved thermal stability, UV-blocking performance, hydrophobicity and mechanical properties. In addition, DA- g -P(MMA- co -LMA) polymers showed good mechanical and elastic properties with highest tensile strength of 5.10 ± 0.87 MPa and strain of 513 ± 32 % and elastic strain recovery above 70 % which was comparable with other reported sustainable thermoplastic elastomers (TPEs) polymers. This work demonstrates an alternative avenue for rosin-based polymer design to achieve the improvement in thermal, UV-blocking and mechanical performance.
研究领域:
材料科学
·
化学工程
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产品关联
DOI
高影响因子
POLYMER
·
2021
·
IF 4.43
Chao Wang,Xipeng Song,Yawei Liu,Chunhua Zhang
Polyorganosiloxane (silicone)-contaminated wastewater is a typical chemical wastewater that causes serious environmental pollution, is difficult to treat and has a high separation cost due to its toxicity, high chemical stability, and low surface tension. To liminate this pollution, a low-cost ultrafiltration membrane with excellent separation properties was successfully fabricated. First, to prepare the membrane, an amphiphilic polymer of poly(vinyl chloride)-g-poly(vinyl pyrrolidone) (PVC-g-PVP) was synthesized by atom transfer radical polymerization (ATRP) over an inexpensive catalyst/ligand system of CuCl/benzyl dimethylamine (BDMA). Thereafter, the membrane was fabricated by regulating the aggregation of the PVC-g-PVP amphiphilic polymer in its casting solution by increasing the solution temperature. The surface smoothness and hydrophilicity of the resulting PVC-g-PVP membrane were better than those of the pristine PVC membrane. Furthermore, the separation and anti-fouling performances of the resulting membrane for silicone-contaminated wastewater were better than those of the pristine PVC membrane. The permeation flux presented an increment of approximately 233%, with a silicone rejection rate was of approximately 100%, and the flux recovery ratio of approximately 72.7% after three cycling tests, which was an increase of approximately 423%. The integration of inexpensive BDMA was used as ligand for the synthesis of PVC-g-PVP, and hydrophilic PVC-g-PVP membrane was fabricated by regulating the aggregation of the PVC-g-PVP in solution, this work provided a new way for the fabrication of amphiphilic polymer membrane with potential application in chemical wastewater remediation.
研究领域:
环境科学
·
材料科学
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产品关联
DOI
高影响因子
CELLULOSE
·
2021
·Vol. 28
·期 14
·9413-9424
·
IF 5.044
Wu Rina,Li Yanan,Shi Jiahui,Wang Qiuyu
Cellulosic materials show great superiority in oil/water separation owing to abundance, low cost, renewability and biodegradability. Herein, silylated cellulose graft copolymer (C-PGMA-S) was prepared by combing ATRP grafting of glycidyl methacrylate with ring-opening reaction between epoxy groups in grafted chains and amine groups in (3-Aminopropyl)- triethoxysilane. C-PGMA-S was utilized to construct hydrophobic surface for filter paper substrate conveniently through dipping and drying. As the curing proceeded, water contact angle of the modified paper increased from 77° to 128°. Inhomogeneous coverage on the pores and micro-scaled irregular concavities were observed by scanning electron microscopy (SEM) on modified paper, resulting in decreased porosity and increased roughness. Moreover, bursting strength was improved by 27%. An efficiency of 96.8% was achieved using the modified paper for oil/water separation. The modified paper exhibited moderate reusability with the separation efficiency remaining over 90% after reuse for 8 cycles without renewing process and good stability against pH variations. The C-PGMA-S modified filter paper, which is fluorine-free, might be a potential candidate for oil/water separation.
研究领域:
材料科学
·
化学工程
·
环境科学
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产品关联
DOI
高影响因子
ANALYST
·
2023
·Vol. 148
·期 24
·6359-6368
·
IF 4.2
Shipeng Jiang,Mingyang Sun,Peiran Meng,Xiaoyu Zhang,Yue Sun
Highly sensitive testing of trace lipopolysaccharides (LPS) is very important due to their high toxicity to the human body. Here, an ultrasensitive electrochemical sensor requiring only 5 μL solution was developed for LPS detection via triple-signal amplification based on ultrafast atom transfer radical polymerization (UATRP) and a Au ultramicroelectrode (UME). Firstly, the Au UME was modified with gold nanoparticles (nAu) and an LPS aptamer (Apt) in turn. When the Apt recognized LPS, the ATRP initiator of 4-(bromomethyl)phenylboronic acid (BPA) could be tethered to the electrode by covalent cross-linking between the phenylboronic acid moiety and the cis-diol site of LPS. Then UATRP was conducted for 2.5 min with nitrogen-doped carbon quantum dots (N-CQDs) as the photocatalyst and methylacrolein (MLA) as the monomer. After the electroactive probes of Ag nanoparticles (AgNPs) were formed on the surface of poly(MLA) by the silver mirror reaction, the electrochemical sensor was successfully prepared. Under the optimal conditions, the sensor exhibited a lower detection limit and a wider linear range when it was compared with a similar assay for LPS. In particular, the LOD of 7.99 × 10−2 pg mL−1 was better than that of the limulus amoebocyte lysate (LAL)-based technique, which is the gold standard for LPS detection. In the end, the sensor reported in this paper showed good selectivity and satisfactory feasibility for LPS detection in real biological samples and food products. The results obtained from the drug, blood and potable water samples laid a strong foundation for its clinical applications and application in other fields.
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产品关联
DOI
PubMed
高影响因子
ANALYST
·
2023
·Vol. 148
·期 10
·2352-2361
·
IF 4.2
Xiwen Li,Peiran Meng,Mingyang Sun,Yue Chen,Zhiyi Song,Xinyao Wang,Na Li,Yue Sun
Phospholipase C (PLC) has important biological functions in specific cancer types, immune disorders and neurodegeneration. Here, an ultrasensitive electrochemical sensor for PLC was developed via signal amplification based on breathing atom transfer radical polymerization (ATRP). First, phosphatidylethanolamine (PE) was immobilized on the surface of a gold electrode by L-cysteine and cross-linker. Then, PE was specially hydrolyzed by PLC to obtain the phosphate groups and tethered with the ATRP initiator α-bromophenacetic acid (BPAA) by the coordination action of Zr4+. After the breathing ATRP, a large number of electroactive monomers (ferrocenylmethyl methacrylate, FcMMA) were successfully grafted from BPAA. The experimental results indicated that the detection signal of the obtained electrode (sensor) was proportional to the concentration of PLC. The sensor showed a low detection limit of 0.270 U L−1 and a wide linear range of 1–40 U L−1 (R2 = 0.997). Most importantly, the sensor was successfully applied to detect PLC in breast cancer cells (MCF-7, MDA-MB-231) and nontumor cells (MCF-10A). The value obtained by our electrochemical sensor had no obvious difference from that determined by the commercial ELISA kit. These results showed that the fabricated PLC sensor had acceptable potential in clinical applications.
研究领域:
生物化学
·
生物技术
·
分析化学
·
癌症研究
·
免疫学
·
神经科学
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产品关联
DOI
PubMed
高影响因子
MICROCHEMICAL JOURNAL
·
2023
·
IF 4.8
Xiwen Li,Zuan Yang,Peiran Meng,Shipeng Jiang,Mingyang Sun,Xiaoyu Zhang,Yue Sun
An ultrasensitive electrochemical sensor for phospholipase C (PLC) was developed via dual signal amplification based on metal-free visible-light induced atom transfer radical polymerization (MVL ATRP) and Ag nanoparticles (AgNPs). The environment-friendly MVL ATRP method was used to form aldehyde based polymers on the electrode surface, providing more reaction sites for further silver mirror reaction. In addition, a large number of electrically active AgNPs were obtained through efficient and low-cost silver mirror reaction, which improved the detection signal. Under the optimal conditions, the proposed sensor showed a linear response from 1 mU/L to 10 6 mU/L for PLC with a detection limit of 0.1032 mU/L (S/N = 3). Most importantly, the sensor was successfully applied to detect PLC in non-tumor cells (MCF-10A) and tumor cells (MCF-7, MDA-MB-231). The obtained values had no obvious difference with that determined by commercial ELISA kit. When 0.5–10 U/L standard PLC were added into the above cells extracts, the recovery rate was between 96.1% and 102.9%, and the RSD was less than 4.02%. The results showed that the fabricated PLC sensor had acceptable potential in clinical applications.
研究领域:
分析化学
·
生物化学
·
纳米技术
·
生物医学工程
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产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）