JOURNAL OF THE ELECTROCHEMICAL SOCIETY
·
2024
·Vol. 171
·期 8
·087521
·
IF 3.1
Qiaoning Dang,Bolu Sun,Hongxia Shi,Quhuan Ma,Yuhong Liu,Haoye Zou,Xinlan Wang,Xuanxiu Da,Miao Zhou,Ying Lv,Lin Yang,Xiaofeng Shi
Fusarium acid (FSA) serves as a highly sensitive biomarker for the early warning of bulb rot in Lanzhou lily caused by Fusarium oxysporum infection. Realizing its highly sensitive, accurate, and rapid detection is crucial for the early warning and control of lily bulb rot. Herein, an enzyme biosensor based on the enzyme inhibition principle was developed for the rapid detection of FSA. Firstly, chitosan-functionalized carboxylated multi-walled carbon nanotubes (CS@cMWCNTS) and gold nanoparticles (AuNPs) were modified on a bare electrode by drop coating and electrochemical deposition methods, respectively. Subsequently, dopamine β-hydroxylase (DBH) was firmly immobilized on the electrode surface through the gold-sulfur bond. Because the activity of DBH can be inhibited by FSA, which leads to a noticeable change in the signal response during the conversion of dopamine (DA) to norepinephrine (NA). Under optimized experimental conditions, the sensor exhibits an excellent linear relationship in the concentration range of 1.00 × 10−5 μg ml−1 ∼ 1.00 × 103 μg ml−1 with the correlation coefficient of 0.9856 and the detection limit is 4.60pg ml−1. Additionally, the sensor showed good stability, repeatability (RSD 1.85%), and selectivity. The method was applied to analyze FSA in the extraction from Lanzhou lily with a recovery higher than 97.59% and RSD less than 1.383%. This method enables the highly sensitive and rapid detection of FSA in real samples, and provides scientific basis and technical support for early warning of diseases and accurate implementation of prevention and control strategies in crop planting and production.
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MICROCHEMICAL JOURNAL
·
2024
·
IF 4.9
Fangfang Zhou,Yue Huang,Qian Wang,Yijie Wang
Antibiotic residue in food is still a serious health hazard to human with the various and severe adverse effects, so ultrasensitive detection of antibiotic residue becomes increasingly significant. Herein, a homogenous electrochemical biosensor is established for ultrasensitive detection of antibiotic ofloxacin (OFL) based on AuNPs-enhanced synergistic amplification of dual enzymes. Target OFL is ingeniously designed to initiate the synergistic effect of exonuclease III-catalyzed DNA cycle and terminal deoxynucleotidyl transferase-mediated polymerization, producing a great many polyA@AuNPs which subsequently adsorb onto the surface of gold electrode and finally lead to highly amplified electrochemical signal by binding with numerous electroactive indicator RuHex. Benefiting from the collaboratively multiple signal amplifications enhanced by AuNPs with good electrical conductivity and probe loading capacity, the constructed biosensor exhibits excellent quantitative detection performance for OFL with a linear range of 0.008 to 100 ng mL −1 and a limit of detection as low as 2.73 pg mL −1 . Furthermore, the detection system accomplished in homogeneous solution without complex chemical modification and rebuilding of sensing architectures on the electrode endows the biosensor with the advantages of convenient operation, good reproducibility and stability. Moreover, the biosensor shows satisfactory analytical performance for OFL detection in real food samples, providing a potential approach for practical application.
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Biosensors-Basel
·
2025
·Vol. 15
·期 1
·30
·
IF 5.6
Zhaoyang Zhou,Xingyue Chen,Shuang Jiang,Zhuoer Chen,Sixian Wang,Yueyang Ren,Xiaodong Fan,Tao Le
Sulfaquinoxaline (SQX) is widely utilized in aquaculture and animal husbandry due to its broad antimicrobial spectrum and low cost. However, it is difficult to degrade, and there are relevant residues in the aquatic environment, which could be harmful to both the ecological environment and human health. As a new recognition molecule, the aptamer can be recognized with SQX with high affinity and specificity, and the aptamer is no longer adsorbed to AuNPs after binding to SQX, which weakens the catalytic effect of AuNPs. Consequently, an aptasensor for the detection of SQX was successfully developed. This aptasensor exhibits a linear range of 40–640 ng/mL, with a detection limit of 36.95 ng/mL, demonstrating both sensitivity and selectivity. The recoveries of this aptasensor in water samples ranged from 90 to 109.9%, which was quite in line with high-performance liquid chromatography. These findings suggest that the aptasensor is a valuable tool for detecting SQX in aqueous environmental samples.
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PubMed
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Analytical Methods
·
2024
·
IF 2.7
Wenxia Xue,Jihong Fu,Yaxue Zhang,Shuxian Ren,Guoqi Liu
Surface-enhanced Raman scattering (SERS) enables pesticide residue monitoring to become facile and efficient. In this study, a core–shell structured gold nanoparticles@ZnCo metal–organic framework (AuNPs@ZnCo-MOF) SERS substrate was designed and successfully synthesized for efficient and selective detection of thiram. The bimetallic ZnCo-MOF shell can not only enrich the targeted molecules in the electromagnetic field because of its excellent absorptive capacity, but also act as a stabilized matrix for protecting the AuNPs from aggregation. The AuNPs@ZnCo-MOFs exhibited a high enhancement factor (EF) of 3.51 × 106 and a low detection limit of 1 × 10−7 mol L−1. Besides, the substrate material showed exceptional stability for up to 28 days at room temperature. The AuNPs@ZnCo-MOFs were used to detect thiram which displayed wide linearity (1 × 10−7 to 1 × 10−4 mol L−1) and high recoveries (83.45–99.61%). Moreover, the AuNPs@ZnCo-MOF SERS substrate exhibited excellent anti-interference ability and size selectivity for the target molecules. These indicate that the AuNPs@ZnCo-MOF substrate has great potential for the detection of thiram residues in practical applications.
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PubMed
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FOOD CHEMISTRY
·
2024
·
IF 8.5
Jiani Zhang,Xiaohui Guo,Jian Zhang,Xiaobing Guo,Yuying Xu,Lijuan Chen
Phoxim, extensively utilized in agriculture as an organothiophosphate insecticide, has the potential to cause neurotoxicity and pose human health hazards. In this study, an electrochemical enzyme biosensor based on Ti 3 C 2 MXene/MoS 2 @AuNPs/AChE was constructed for the sensitive detection of phoxim. The two-dimensional multilayer structure of Ti 3 C 2 MXene provides a robust framework for MoS 2 , leading to an expansion of the specific surface area and effectively preventing re-stacking of Ti 3 C 2 MXene. Additionally, the synergistic effect of self-reduced grown AuNPs with MoS 2 further improves the electrical conductivity of the composites, while the robust framework provides a favorable microenvironment for immobilization of enzyme molecules. Ti 3 C 2 MXene/MoS 2 @AuNPs electrochemical enzyme sensor showed a significant response to phoxim in the range of 1 × 10 −13 M to 1 × 10 −7 M with a detection limit of 5.29 × 10 −15 M. Moreover, the sensor demonstrated excellent repeatability, reproducibility, and stability, thereby showing its promising potential for real sample detection.
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PubMed
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CARBON
·
2025
·
IF 11.6
Shaopeng Xie,Dezhi Yang,Qiulan Li,Jitao Li,Dubo Zhong,Ye Yang,Yaling Yang,Hua Wang
The design of highly selective Raman substrates has always been the key to metal ion detection using surface-enhanced Raman spectroscopy (SERS). Here, a solution volatilization method (SVM) by reducing carbon dots (CDs) was proposed to prepare highly selective gold nanoparticle (AuNPs) as Raman substrates for the specific detection of Hg 2+ through SERS. Five CDs-based AuNPs were synthesized by changing the group on the precursor materials for CDs preparation and the secondary reduction with seed method. The results revealed that thiol and hydroxyl groups played a pivotal role in the detection of Hg 2+ . Interestingly, the influence of the chirality, number and type of the groups on the detection of Hg 2+ is substantial. The DTT-CDs/AuNPs-2, prepared from dithiothreitol (DTT) containing two thiol groups and hydroxyl groups, was capable of selectively facilitating the formation of gold amalgamation (Au@HgNPs) with Hg 2+ , without being affected by methylmercury. The Au@HgNPs showed obviously enhanced oxidase (OXD)-like activity, which can oxidize TMB (weakly SERS-active) to ox-TMB (strongly SERS-active) with strong Raman signal. This led to a rapid and specific SERS detection of Hg 2+ with a detection limit of 9.56 nM. This work provides guidance for designing ultra-sensitive and highly specific field analysis of heavy metal ions through the modification of CDs' functional groups.
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CHEMICAL ENGINEERING JOURNAL
·
2025
·
IF 13.2
Bohan Zhang,Jinglei Yang,Qiaojun Jiang,Ruping Zheng,Liting Qiu,Jingxin Chen,Ruiyun You,Zixing Yan,Yudong Lu
In clinical settings, there is a significant demand for the monitoring of therapeutic drugs. Surface-enhanced Raman spectroscopy (SERS) has shown considerable potential in the field of drug analysis. Designing SERS sensors with high sensitivity, strong stability, excellent reproducibility is a challenging and fascinating goal in the field of chemical measurement. In this study, a simple and portable AuNPs/C-CNF/PVA hydrogel SERS sensor was developed, composed of gold nanoparticles (AuNPs), carboxylated cellulose nanofibers (C-CNF) and polyvinyl alcohol (PVA). Due to the “screening” effect of the hydrogel network, it is capable of separating hydrophobic molecules from macromolecules and enabling the enrichment of hydrophilic small molecules. When used to analyze 6-thioguanine (6-TG) and methylene blue (MB) in plasma, the AuNPs/C-CNF/PVA hydrogel SERS sensor showed limit of detection (LOD) as low as 7.9 × 10 −8 M for 6-TG and 3.3 × 10 −9 M for MB. Based on this technology, in blood spiking experiments using clinical samples, the recovery rate for 6-TG ranged from 99.16 % to 103.51 %, while the recovery rate for MB ranged from 93.13 % to 101.70 %. Therefore, the AuNPs/C-CNF/PVA hydrogel SERS sensor enables ultra-sensitive, integrated detection of drugs in plasma through separation and enrichment, without the need for complex pretreatment steps. This approach holds great promise for application in clinical therapeutic drug monitoring.
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SENSORS AND ACTUATORS B-CHEMICAL
·
2024
·
IF 8
Jialin Zhang,Ming Zhou,Linna Yang,Bin Huang,Kangqiang Lu,Herui Wen,Jiali Ren
Foodborne bacterial infections pose serious threats to human health, leading to illnesses and even fatalities. The key to solve this problem is to develop fast and effective sensor for sensitive detection of bacteria. In this study, an electrochemical biosensor based on magnetic nanocomposite material Fe 3 O 4 @COF-AuNPs and trigging isothermal circular amplification (TICA) was constructed for detection of foodborne bacteria. In this design, a hairpin aptamer probe (HAP)-bound DNA monolayer was designed, serving as the substrate for the electrode to increase surface area. The Escherichia coli ( E. coli ) was selected as the target. The presence of E. coli caused the configuration change of the HAP and allowed the occurrence of TICA with the use of the Klenow Fragment polymerase (KF-Poly), thus the HAPs were away from the DNA monolayer. After adding the catalytic magnetic nanocomposite material Fe 3 O 4 @COF-AuNPs, it was immobilized on the DNA monolayer of the electrode through DNA complementary pairing and exhibited high electrocatalytic behavior towards the reduction of 4-nitrophenol. This enabled ultrasensitive detection of E. coli , with the detection limit (LOD) as low as 10 CFU/mL. The detection time was 1 h. This designed electrochemical sensor showed high selectivity, stability, and reproducibility, which offered a new avenue for detection of foodborne bacteria.
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SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
·
2024
·
IF 4.3
Kexiang Li,Hong Li,Qian Zhang,Dezhi Yang,Yaling Yang
In this work, a facile synthesis method for dopamine carbon dots-based Au nanoparticles (DA-CDs/AuNPs) by seed gold method was reported as the surface enhanced Raman scattering (SERS) booster. DA-CDs with rich in surface functional groups was synthesized using dopamine, citric acid and ethylenediamine as precursors by a facile hydrothermal method, and can be used as the capping agents and reducing agents for the synthesis of DA-CDs/AuNPs. Due to the electromagnetic “hot spots” effect, DA-CDs/AuNPs with core–shell structure exhibited strong SERS activity. Based on the specific interaction of DA-CDs/AuNPs and fenamidone, a detection method of fenamidone was established with a low detection limit of 0.05 μg/mL. Finally, the SERS sensor was successfully applied to the detection of fenamidone in fruit with recoveries between 90.6 % and 98.7 %. The method here proposed can be reliably applied for fenamidone detection on fruits.
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International Journal of Electrochemical Science
·
2023
·
IF 1.5
Mingyan Chai
Foodborne pathogenic bacteria can cause food poisoning or infection. Salmonella is an aerobic or partly anaerobic gram-negative bacterium that enters the body with food, invades the intestine and releases large amounts of endotoxin. The endotoxin acts as a pyrogen that leads to an increase in body temperature and acts on the intestinal mucosa to trigger systemic inflammation and toxic symptoms in the host. The development of novel methods and systems for the monitoring and detection of foodborne pathogenic bacteria is of great significance for food safety, especially the development of simple, high-throughput and highly sensitive rapid detection techniques. This work proposes a novel aptamer sensor based on reduced graphene oxide (rGO)-AuNPs for the detection of Salmonella in food. Firstly the rGO and AuNPs were modified on the surface of glassy carbon electrode in turn, and then a highly sensitive sensor was made by designing a segment of thiolated aptamer probe that could specifically capture Salmonella . The high electron transport ability of rGO and the advantage of large specific surface area of AuNPs were adopted to achieve signal amplification and improve the detection capability. In the presence of Salmonella , the aptamer traps and immobilizes Salmonella on the surface of the electrode, resulting in a change in the immediate current in the sensor and realizing quantitative detection of Salmonella based on the change in electrical signal. Under the optimized conditions, the response current of the constructed aptamer sensor is linearly related to the logarithmic value of Salmonella concentration, with a linear range of 6×10 2 cfu/mL~6×10 7 cfu/mL and a detection limit of 200 cfu/mL (S/N=3). Meanwhile, this sensor has been successfully applied in the detection of Salmonella in pork and beef.
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CARBOHYDRATE POLYMERS
·
2022
·
IF 10.723
Lu Zheng,Bin Gu,Shanshan Li,Bichong Luo,Yuming Wen,Meiwan Chen,Xiaoyun Li,Zhengang Zha,Huan-Tian Zhang,Xiaoying Wang
The development of shape-memory sponge dressings with functions, such as hemostasis , antibacterial activity , and wound healing, is of great significance in clinical applications. Herein, a novel AuNPs@corn stalk/chitin composite sponge (CCAu) was fabricated by crosslinking the chitin matrix with corn stalk-embedded gold nanoparticles (AuNPs). The addition of AuNPs@corn stalk gave the porous chitin sponge shape-recovery ability with improved softness, porosity, and water absorption. Correspondingly, the composite sponge showed better hemostatic effects than commercial PVF sponges. The photothermal effect of AuNPs endowed the composite sponge with excellent antibacterial activity. In addition, the wound treated with composite sponge containing antioxidant AuNPs exhibited a significantly faster wound healing rate (reaching 41.6 % on day 3) than the CH (33.2 %) and control (12.6 %) group through promoting cell migration, angiogenesis and collagen deposition. Therefore, the multifunctional composite sponge with great biocompatibility in this work provides a potential strategy for wound healing.
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JOURNAL OF NANOPARTICLE RESEARCH
·
2016
·Vol. 18
·期 6
·1-11
·
IF 2.101
Li Runming,Li Zhiyuan,Wu Qiang,Li Dongfeng,Shi Jiahua,Chen Yuewen,Yu Shuling,Ding Tao,Qiao Congzhen
Abstract Oxidative polymerization of aniline was carried out in ethanol using chloroauric acid (HAuCl 4 ) as the oxidant. Simultaneous reduction of HAuCl 4 and formation of gold nanoparticles (AuNPs) and polyaniline (PANI) composite nanospheres (AuNPs@PANI nanospheres) were achieved without using any templates or structure-directing agents. The composite nanospheres are uniformly distributed with an average diameter of about 400 nm, in which the ultrafine AuNPs with size of about 2–4 nm were evenly embedded in the PANI matrix which acted as the dispersing agent and stabilizer of AuNPs. In addition, the catalytic performance of these composite nanospheres towards the reduction of 4-nitrophenol in the presence of NaBH 4 was studied. Furthermore, the possible formation mechanism and catalytic mechanism of the self-assembled AuNPs@PANI nanospheres were also discussed. Graphical Abstract
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危险品化学品经营许可证（带存储）