Journal of Materials Chemistry A
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2018
·Vol. 7
·期 1
·157-164
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IF 9.931
Danyang Zhao,Yuyang Tian,Xiaofei Jing,Yun Lu,Guangshan Zhu
Bisphenol A (BPA), an endocrine disrupting compound (EDC) that affects the human nervous system, is used worldwide and is difficult to remove from water. PAF-1@cellulose nanofibril (CNF) composite aerogels are prepared through a double-cross-linking method and applied as adsorbents to remove BPA from water. With the successful combination of the high surface area of PAF-1 and the bulk skeleton of CNFs, as well as strong interactions between BPA molecules and the networks, the resulting composite aerogels can remove 77.7% of BPA, dissolved in water, within 10 seconds at an adsorption rate of 532.2 mg g−1 min−1, and their removal efficiency reaches 97.0% after 1 hour of adsorption. The adsorption capacity of PAF-1@CNF composite aerogels towards BPA is estimated to be as high as 1000 mg g−1 according to the Langmuir adsorption model.
研究领域:
环境科学
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材料科学
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化学工程
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水处理
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DOI
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2024
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IF 7.7
Yue Du,Chong-Mei Xu,Yu-Min Zhang,Zheng-Xuan Pan,Feng-Shan Wang,Hong-Ming Yang,Jin-Bao Tang
Covalent and oriented immobilization of antibodies (Abs) can substantially improve the sensitivity and stability of solid-phase immunoassays . By modifying the natural Abs with functional groups that provide unique handles for further conjugation, Abs could be immobilized onto the solid matrices with uniform orientation. Herein, an effective approach for Fc-specific modification of Abs was developed for the oriented and covalent immobilization of Abs. Twelve photoreactive Z -domain variants, incorporated with a photoactivable probe ( p -benzoyl- L -phenylalanine, Bpa) at different positions and carrying a C-terminal Cys-tag (i.e. Z Bpa -Cys variants), were individually constructed and produced in Escherichia coli and tested for photo-cross-linking to various IgGs. The different Z Bpa -Cys variants demonstrated large differences in photo-conjugation efficiency for the tested IgGs. The conjugation efficiencies of 17th Z Bpa -Cys ranged from 90 % to nearly 100 % for rabbit IgG and mouse IgG 2a , IgG 2b and IgG 3 . Other variants, including 5th Z Bpa -Cys, 18th Z Bpa -Cys, 32th Z Bpa -Cys, and 35th Z Bpa -Cys, also displayed conjugation efficiencies of 61 %–83 % for mouse IgG 1 , IgG 2a and IgG 3 . Subsequently, the photo-modified Abs, namely IgG–Cys conjugates, were covalently immobilized onto a maleimide group-functionalized solid-phase carrier on the basis of the reaction of sulfhydryl and maleimide . Thus, a generic platform for the controlled and oriented immobilization of Abs was developed, and the efficacy and potential of the proposed approach for sensitive immunoassays was demonstrated by detecting human α-fetoprotein.
研究领域:
生物化学
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免疫学
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生物技术
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分析化学
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DOI
PubMed
高影响因子
Analytical Methods
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2019
·Vol. 11
·期 43
·5613-5622
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IF 2.378
Xiaojiao Wang,Yang Li,Yuanzhen Zhou,Yijia He
Bisphenol A (BPA) is one of the most prevalent environmental endocrine disrupting chemicals. In this present paper, a facile BPA sensor was successfully fabricated based on a La-doped Yb2O3 nanorod modified carbon paste electrode (La–Yb2O3/CPE). La-doped Yb2O3 nanorods were synthesized using a one step hydrothermal synthesis method and further investigated by X-ray diffraction (XRD), scanning electron microscopy (SEM) and energy dispersive X-ray spectroscopy (EDS). Electrochemical impedance spectroscopy (EIS), cyclic voltammetry (CV) and amperometric response analysis were applied to characterize the electrochemical properties of the sensor. Under the optimal conditions, the oxidation current increased linearly with increasing the concentration of BPA in the range of 0.04 to 15 μM with a detection limit of 0.013 μM. The prepared La-doped Yb2O3 nanomaterial modified electrode showed good reproducibility, stability and anti-interference ability. Furthermore, this newly developed sensor was further adopted to measure BPA in tap water and milk samples. The possible electrocatalytic sensing mechanism is discussed.
研究领域:
环境科学
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分析化学
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纳米技术
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电化学
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DOI
高影响因子
ANALYST
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2019
·Vol. 144
·期 19
·5691-5699
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IF 4.019
Manman Li,Zhuangzhuang Guo,Xiaoke Zheng,Huaixia Yang,Weisheng Feng,Jinming Kong
Herein, a novel aptasensor was constructed for ultrasensitive detection of bisphenol A (BPA). In this method, an electrochemically mediated atom transfer radical polymerization (eATRP) signal amplification strategy was applied to BPA detection for the first time. The 5′-end modified sulfhydryl group and the 3′-end modified azide group hairpin DNA were immobilized on a gold electrode through an Au–S bond. The double-stranded DNA was formed by the hybridization of an aptamer and a single-stranded DNA partially paired with the hairpin DNA. In the presence of BPA, the aptamer combined with BPA and the single-stranded DNA was released to open the hairpin structure, making the azide groups at the 3′ end exposed. Subsequently the initiator of eATRP was introduced into hairpin DNA through click chemistry reaction and eATRP was conducted for the polymerization of the electroactive probe ferrocene methyl methacrylate (FMMA). As a result, the ultrasensitive detection of BPA was realized, and the detection limit of this aptasensor was as low as 59 aM and a good selectivity was obtained in the presence of 100-fold structural analogs. The application of this aptasensor was evaluated by detecting BPA in pure water samples, and recoveries were in the range of 95.23–98.40%, holding promising applications in biological analysis.
研究领域:
分析化学
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环境科学
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电化学
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纳米技术
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生物技术
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DOI
PubMed
高影响因子
ADVANCED FUNCTIONAL MATERIALS
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2025
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IF 19
Mengxue Wang,Lingsha Li,Haipeng Yan,Yong You,Xiaohong He,Zhongxiang Bai,Ying Li,Kui Li,Xulin Yang,Pan Wang
Separating oil-water emulsions in harsh conditions poses a substantial challenge for filtration membrane systems. In response, this study presented a continuous electrospinning technique to fabricate a robust bifacial composite membrane. Two types of high-performance polyarylene ether nitrile (PEN) polymers, PEN-BPA and PEN-B/P, with different wettability, are employed to construct the AB bifacial structure. Further, the A-side is modified with hydrophilic polydopamine (PDA) and antibacterial silver nanoparticles (Ag NPs) to prepare Ag@PDA/PEN-BPA, while the B-side is altered with photocatalytic titanium dioxide nanoparticles (TiO 2 ) to prepare TiO 2 /PEN-B/P. The resulting Ag@PDA/PEN-TiO 2 /PEN-B/P bifacial membrane demonstrates remarkable photocatalytic degradation efficiency, particularly for MB, reaching 99.8% degradation efficiency. Moreover, the permeation flux and oil-water emulsion separation rejection rate of the membrane reach 5180.34 L m − 2 ·h −1 and 99%. The PEN substrates are not only used to construct the AB bifacial structure but also to enhance the membrane's toughness and durability, ensuring stability in high-temperature and corrosive environments, with a rejection rate reaching 99% after 10 reuse cycles. Furthermore, integrating Ag NPs imparts excellent antibacterial properties to the membrane, sustaining a 99.99% antibacterial rate after oil-water emulsion separation, photocatalytic dye degradation, and exposure to high-temperature NaCl immersion. This study offers novel insights into multifunctional water treatment materials in harsh environments.
研究领域:
材料科学
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环境工程
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纳米技术
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水处理
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膜技术
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DOI
高影响因子
ENVIRONMENT INTERNATIONAL
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2020
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IF 7.577
Miaoling Lin,Rui Hua,Jing Ma,Yao Zhou,Pei Li,Xiya Xu,Zhiqiang Yu,Song Quan
Background Bisphenol A (BPA) is a widespread endocrine-disrupting chemical with estrogen like effects, which could interfere with the human reproductive system by disrupting the normal function of granulosa cells (GCs) leading to abnormal ovarian function. However, the mechanism of its toxicity on human GCs has not been clearly described thus far. Methods 106 normogonadotropic infertile women undergoing their first in-vitro fertilization-embryo transfer (IVF-ET) cycle were recruited. Urinary BPA level and the early outcomes of IVF-ET were analysed. Patients were divided to low and high BPA exposure groups using the median urinary BPA concentration as the cut-off value. In-vivo and in-vitro studies were conducted using mice and human granulosa cell line (KGN cells). Female Kunming mice approximately 6–8 weeks of age were poisoned with BPA at different dosages (1, 10 or 100 μg/kg) by oral gavage once daily for 2 weeks, while KGN cells were exposed to BPA at the concentration of 1, 10 or 100 nM for 24 h, 48 h or 72 h. BPA-induced ovarian morphologic changes were analysed by histopathology investigation. Cell viability and apoptosis were evaluated using CCK-8, TUNEL and flowcytometric, respectively. Hormone levels were determined using ELISA and the molecular mechanism studies were conducted using immunofluorescence, RT-PCR and western blots. Results The oocyte retrieval rate, maturation rate and embryo implantation rate significantly decreased with the higher level of urinary BPA concentration. Peak E2 level was lower in high BPA group, but no statistical significance could be observed. In BPA treated mice, cystic dilation of the follicles with a decreased number of GCs could be observed histopathologically. Decreased E2, P4 and AMH level and GCs autophagy could be detected both in-vivo and in-vitro with the activation of AMPK/mTOR/ULK1 signalling pathway. As being confirmed in KGN cells, phosphorylated AMPK and ULK1 increased while phosphorylated mTOR decreased, and by inhibition autophagy using knockdown of AMPK or 3-MA, adverse effects of BPA exposure in-vitro could be reversed. Conclusion BPA exposure might abnormally influence human ovarian functions leading to abnormal folliculogenesis by activation of autophagy in GCs through AMPK/mTOR/ULK1 pathway.
研究领域:
内分泌学
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生殖生物学
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毒理学
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细胞生物学
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分子生物学
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DOI
PubMed
高影响因子
VIBRATIONAL SPECTROSCOPY
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2021
·
IF 2.507
Lei Zhang,Yingshan Chen,Qin Zhu,Wenjin Ji,Suqing Zhao
Bisphenol A (BPA) is a typical endocrine-disrupting chemical (EDC), which has been banned for food packing and baby bottle material due to its potential endocrine harm to human body. In this paper, an easy and rapid immunochromatographic assay (ICA) combined with the technology of surface-enhanced Raman spectroscopy (SERS) was fabricated to improve the sensitivity and rapidity for quantitative determination of BPA. After 10 min of a quick chromatographic process, the specific Raman signal on T line of the test strip was measured. Compared with the traditional ICA, the SERS-ICA realized quantitative test and showed the limit of detection (LOD) was 0.1 ng/mL, getting approximately 300-fold lower. The method displayed high specificity and precision with RSD of 4.1%, and the test strip indicated favorable stability for two weeks without loss of activity. For river, tap and commercial drinking water sample, the SERS-ICA results were confirmed by HPLC, and the recoveries of spiked BPA were from 90.80-117.80%. It was shown that the proposed method takes advantages both of SERS and ICA, exhibiting high sensitivity, precision, stability, quantification and rapidity for field test, expecting a widely useful method for various small molecules in food safety, medical diagnosis and environmental samples.
研究领域:
分析化学
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环境科学
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食品安全
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医学诊断
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产品关联
DOI
高影响因子
ADVANCED FUNCTIONAL MATERIALS
·
2025
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IF 19
Zhixin Wang,Yuanpeng Fu,Tianzhao Bu,Yuqi Qiu,Bo Pang,Qingyang Zheng,Sen Zhou,Yangyang Li,Xiling Xiao,Zhouping Yin,Hao Wu
Quantitative and objective assessment of muscle spasticity grades in post-stroke patients plays a pivotal role for physicians and patients in adjusting rehabilitation plans and preventing secondary complications. However, the development of such methods for spasticity detection and assessment is hindered by the subjectivity of conventional assessment methods, the limited clinical applicability of digital-devices, and the structural complexity of sensors used for monitoring muscle strength. Here, a multimodal on-skin sensor is developed, enabling simultaneous acquisition of surface electromyography (sEMG) and triboelectric signals, through wavelet analysis, the signal from the sensor can comprehensively reflect the bioelectrical and biomechanical characteristics of human motion. The high-performance triboelectric materials (BPA NPs) with metal-organic–inorganic core-shell structures are synthesized to enhance the signal quality of the multimodal on-skin sensor by leveraging the self-polymerization ability and weak reducibility of dopamine. The sensitivity of the multimodal on-skin sensor is improved by designing a radially arranged micro-cone array with gradient heights. Additionally, a quantitative spasticity assessment strategy is developed by integrating muscle co-activation coefficients (Index 1) and antagonistic efficacy metrics (Index 2), which are strongly correlated with the Modified Ashworth Scale scores. The multimodal on-skin sensor with the proposed assessment strategy enables quantitative assessment of spasticity in 9 spasticity patients.
研究领域:
生物医学工程
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可穿戴传感器
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神经康复
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信号处理
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智能材料
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产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）