JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY A-CHEMISTRY
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2025
·
IF 4.7
Na Liu,Yanwei Su,Siyi Zheng,Yuchen Tang,Chunxia Yu,Yuangang Li,Lihua Shen
Sulfur nanosheets (S-NSs), as a novel photocatalyst, was designed to composite with graphite phase nitrogen carbide (g-C 3 N 4 ) to form a kind of new S-NSs/g-C 3 N 4 (CNS) photocatalyst. The modulating effect of bovine serum albumin (BSA) or poly (3,4-ethylenedioxythiao)-polystyrene sulfonic acid (PEDOT:PSS) ligands on the photocatalytic activity of CNS nanocomposite was studied. The ligands mainly affect the band gap and conduction band potential of the photocatalyst, leading to different photocatalytic activities. When BSA was used as a ligand, the combine of S-NSs and g-C 3 N 4 (CNS-B) cannot improve the photocatalytic activity for the degradation of Rhodamine B (RhB). When PEDOT:PSS was used as a ligand, the composite catalysts of CNS-P significantly improved the photocatalytic activity. The degradation efficiency of composite material CNS-P for RhB and Doxycycline hydrochloride (DOX) can reach 97 % (90 min) and 78 % (90 min). Since the combine of S-NSs and g-C 3 N 4 , the photoelectric conversion ability was enhanced and the carrier mobility was accelerated. The composite catalyst enhanced the redox ability and suppressed the recombination of photo generated carriers through Z-type charge transfer. This study give a reference for designing a new photocatalysts of two dimensional materials and also provide a potential feasibility for antibiotic degradation application.
研究领域:
材料科学
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环境科学
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光催化
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DOI
高影响因子
POLYMERS FOR ADVANCED TECHNOLOGIES
·
2018
·Vol. 30
·期 2
·351-359
·
IF 2.137
Jinying Du,Yu Tian,Ning Li,Jun Zhang,Wei Zuo
In order to improve the antifouling performance of PVDF membrane, a novel zinc sulfide/graphene oxide/polyvinylidene fluoride (ZnS/GO/PVDF) composite membrane was prepared by immersed phase inversion method. The surface morphology, crystal structure, photocatalytic activity, and antifouling property of the as-prepared membranes were systematically studied. Results showed that the ZnS/GO/PVDF hybrid membranes were successfully fabricated with uniform surface. The hybrid membrane surface possessed higher hydrophilicity with water contact angle decreasing from 77.1° to 62.2°. The permeability of the hybrid membrane was therefore enhanced from 222.9 to 326.1 L/(m 2 hour). Moreover, bovine serum albumin (BSA) retention experiment showed that the hybrid membrane separation was also promoted by 7.2%. The blending of ZnS and GO enhanced the hydrophilic and photocatalytic performances of PVDF membrane, which mitigated the membrane fouling effectively. This novel hybrid membrane could accelerate the practical application of photocatalytic technology in membrane separation process.
研究领域:
材料科学
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环境工程
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膜技术
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DOI
高影响因子
Analytical Methods
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2018
·Vol. 10
·期 16
·1895-1901
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IF 2.073
Jiawei Li,Pan Li,Minghong Bian,Danqun Huo,Changjun Hou,Hui Qin,Suyi Zhang,Liang Zhang
Cu2+ plays an important role in various fundamental physiological processes in organisms. Although Cu2+ probes have been developed, little research has focused on using a direct redox route to quench fluorescence for Cu2+ detection. Herein, a simple, label-free, cost-effective, and sensitive fluorescence method has been developed for Cu2+ detection. In the absence of Cu2+ and presence of NH3+ and SCN−, H2O2 was employed as an oxidant to corrode BSA–Au NCs, resulting in the disappearance of fluorescence. In contrast, in the presence of Cu2+, red fluorescence was maintained because Cu(NH3)62+ can decompose H2O2. Under optimal conditions, good linear correlation between the fluorescent response and Cu2+ concentrations ranging from 5 to 1250 nM was obtained (R2 = 0.9938). Furthermore, this monitoring method has successfully been applied to real urine, tap water, and sorghum extract samples, demonstrating the potential for field applications. Based on this work, we successfully immobilized BSA–Au NCs on wax-printed paper to accomplish real-time field Cu2+ detection. Fluorescence images were captured under UV light (365 nm), and R-values (red value of RGB color model) were analyzed. The obtained linear relationship between the R-value and the Cu2+ concentration demonstrated the potential of this approach. The developed probe also showed satisfactory selectivity and excellent reproducibility. Although previous methods required a masking agent to protect against interference from increasing Hg2+ concentration, Hg2+ and other interfering ions had a near-negligible effect on results in the present work.
研究领域:
分析化学
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生物医学工程
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环境科学
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DOI
高影响因子
Biosensors-Basel
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2025
·Vol. 15
·期 1
·49
·
IF 5.6
Yijiong Xue,Chengqi Bao,Hui Liu,Fanghui Ma,Minghui Yang,Xiaoqing Li
A fluorescence probe for “switch-on” detection of alkaline phosphatase (ALP) was developed based on Au nanoclusters anchored MnO2nanosheets (Au NCs-MnO2NSs), which were synthesized using bovine serum albumin (BSA) as template through a simple one-pot approach. In the sensing system, MnO2NSs function as both energy acceptors and target identifiers, effectively quenches the fluorescence of Au NCs via fluorescence resonance energy transfer (FRET). The presence of ALP catalyzes the hydrolysis of L-ascorbic acid-2-phosphate (AAP) to ascorbic acid (AA), reducing MnO2NSs to Mn2+and facilitate the fluorescence recovery of Au NCs. The fluorescence assay offers the advantages of facile preparation, cost-effectiveness, good specificity, and high sensitivity. Moreover, the assay exhibits a broad linear range (0.005 U/mL to 8 U/mL) for ALP detection with a remarkable limit of detection of 0.0015 U/mL. Notably, this assay demonstrates promising applicability for detection ALP in human serum samples, thereby providing valuable potential for clinical applications.
研究领域:
生物化学
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分析化学
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医学检测
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DOI
PubMed
高影响因子
DALTON TRANSACTIONS
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2015
·Vol. 44
·期 29
·13112-13118
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IF 4.197
Cai Zhang,Yan-Yan Fu,Xuejun Zhang,Chunshui Yu,Yan Zhao,Shao-Kai Sun
Photothermal therapy as a physical therapeutic approach has greatly attracted research interest due to its negligible systemic effects. Among the various photothermal agents, CuS nanoparticles have been widely used due to their easy preparation, low cost, high stability and strong absorption in the NIR region. However, the ambiguous biotoxicity of CuS nanoparticles limited their bio-application. So it is highly desirable to develop biocompatible CuS photothermal agents with the potential of clinical translation. Herein, we report a novel method to synthesize biocompatible CuS nanoparticles for photothermal therapy using bovine serum albumin (BSA) as a template via mimicking biomaterialization processes. Owing to the inherent biocompatibility of BSA, the toxicity assays in vitro and in vivo showed that BSA–CuS nanoparticles possessed good biocompatibility. In vitro and in vivo photothermal therapies were performed and good results were obtained. The bulk of the HeLa cells treated with BSA–CuS nanoparticles under laser irradiation (808 nm) were killed, and the tumor tissues of mice were also successfully eliminated without causing any obvious systemic damage. In summary, a novel strategy for the synthesis of CuS nanoparticles was developed using BSA as the template, and the excellent biocompatibility and efficient photothermal therapy effects of BSA–CuS nanoparticles show great potential as an ideal photothermal agent for cancer treatment.
研究领域:
生物医学工程
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纳米医学
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肿瘤学
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DOI
PubMed
高影响因子
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
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2025
·
IF 4.6
Zhangsibo Yu,Xue Wei,Le Dai,Wanli Ma,Weiwei Li,Xiu'ai Li,Xiao-Le Han
The rising incidence of neurodegenerative disorders linked to protein fibrillation in aging populations highlights the need for efficient, low-toxicity fibrillation inhibitors. Quercetin, a natural flavonoid, shows potential in both suppressing fibrillation and disaggregating mature fibrils. However, its mechanisms require further investigation. This study examines the dual binding modes of Quercetin to bovine serum albumin (BSA) using spectroscopic and molecular simulation techniques. These approaches reveal hydrophobic-driven complexation and polar interaction-mediated structural interference. Together, they provide insight into its role in amyloid suppression. Through Thioflavin-T (ThT), 8-anilino-1-naphthalenesulfonic acid (ANS), and Congo Red (CR) analysis, we confirm that Quercetin inhibits BSA fibrillation kinetics and prevents amyloid-like aggregation. Ultraviolet-visible (UV–vis) and variable-temperature fluorescence spectroscopy show that static quenching is the dominant interaction. This interaction is driven by entropy and hydrophobic burial at Sudlow site I (subdomain IIA). A 1:1 binding stoichiometry is also supported. Competitive displacement assays and molecular docking reveal that Quercetin binds preferentially to subdomain IIA through π-π stacking and hydrogen bonds (e.g. Trp-214, Ser-192). Scanning electron microscopy (SEM) further validates Quercetin's suppression of fibrillar growth. This study uncovers the interaction mechanism between Quercetin and BSA. It also provides theoretical support for its potential in anti-fibrillation therapy. Additionally, it highlights the advantages of combining spectroscopic and molecular simulation methods to study biomolecular interactions.
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DOI
PubMed
高影响因子
ANALYTICA CHIMICA ACTA
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2025
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IF 6
Jiaqi Niu,Jinhui Liu,Ping Guo,Min Du,Wanqing Wu,Ziyang Zhang,Ye Ning,Dedi Zhou,Haidong Cao
As a common contaminating bacterium, Escherichia coli (E. coli) has caused severe harm in various fields such as food, healthcare, and the environment. Traditional detection methods for Escherichia coli, owing to factors like high cost and low efficiency, are unable to meet the detection requirements at the present stage. Therefore, the development of a simple, rapid, and highly efficient detection method for Escherichia coli represents an urgent issue that needs to be addressed promptly. This study focused on the development of a highly selective molecularly imprinted electrochemical sensor for E. coli. Molecularly imprinted polymers (MIPs) were prepared by electropolymerization on the surface of glassy carbon electrode using o-phenylenediamine as the functional monomer, aminated metal-organic frameworks (MOFs) and carbon nanotubes (CNTs) as the composites, and bovine serum albumin (BSA) was added into the polymer film to attenuate the non-imprinting effect. The linear range of the sensor was 10-10 7 CFU/mL and the detection limit was 5.2 CFU/mL. Good recovery rates were shown in real samples of drinking water, milk and orange juice. In addition, the good binding ability of the E. coli template molecule to the functional monomer o-phenylenediamine was verified by molecular docking and molecular dynamics (MD) simulations. This study was able to detect E. coli in 20 min, which provides a more open idea for the application of rapid detection of E. coli on-site, as well as the construction of molecularly imprinted electrochemical sensors and the analysis of the binding mechanism between template molecules and functional monomers.
研究领域:
环境监测
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食品安全
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生物传感器
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分析化学
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微生物学
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF MEMBRANE SCIENCE
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2026
·
IF 9
Ruoman Ji,Lijuan Gao,Yibo Zan,Xingchi Zhang,Qing Zhao,Ting Wang
A novel chiral mixed matrix membrane (MMM) was prepared by biomimetic mineralization to encapsulate bovine serum albumin (BSA) within ZIF-8, followed by film formation. In this process, a new ratio of ZIF-8 material and two biocomposites with different precursor ratios were synthesized via a one-pot co-precipitation method under ambient conditions. BSA@ZIF-8 (Zn 2+ /2-methylimidazolate (2-mIM) = 40:320 mM) demonstrated the optimal separation of 1-phenylethanol (PE), with an enantiomeric excess ( ee ) of 52.79%. Subsequently, the combination of BSA@ZIF-8 with polyethersulfone (PES) to form a chiral MMM was investigated, and the effects of the concentration of the feed solution, the proportion of biocomposite in the membrane, the thickness of the membrane and the permeation time of the chirality on the enantiomeric separation were examined. The chiral MMM achieved complete enantioseparation ( ee = 100 %). Both the biocomposite-containing membrane and the free biocomposite retained this performance throughout the stability tests, demonstrating that immobilizing BSA within ZIF-8 markedly enhanced the robustness of the biomacromolecule. Finally, molecular docking studies revealed the presence of hydrogen bonding and hydrophobic forces, especially hydrogen bonding, between BSA@ZIF-8 and the racemate, which explained the selective chiral recognition ability of BSA@ZIF-8 towards the racemate PE.
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DOI
高影响因子
JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY B-BIOLOGY
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2016
·
IF 3.035
Xiaoyi Liu,Zhaoxing Ling,Xing Zhou,Farooq Ahmad,Ying Zhou
Butachlor is an effective herbicide to deal with undesired weeds selectively and is used at high levels in Asian countries. However, its interaction and impairment effect on BSA was still not clear. In this study, we investigated the interaction between butachlor and bovine serum albumin (BSA) by multi-spectroscopic methods including UV absorption , circular dichroism (CD) spectra, Fourier transform infrared (FTIR) spectra and fluorescence spectra under physiological conditions (pH = 7.4). The results revealed that there was a static quenching of BSA induced by butachlor stemmed from the formation of complex. Based on thermodynamic data, the interaction of butachlor with BSA was due to happen, and van der Waals force as well as hydrogen bond were the major forces contributed to the interaction. The binding constant K b and number of binding site of butachlor with BSA were 5.158 × 10 5 and 1.372 at 303 K, respectively. The distance r between donor (BSA) and acceptor (butachlor) was 0.113 nm, obtained according to the Förster theory. The results revealed that butachlor induced conformational changes in BSA but the secondary structure of BSA was still retained. In addition, the microenvironment around chromophore residues of BSA, for example, tryptophan , changed as well, resulting from the formation of more hydrogen bonds.
研究领域:
生物化学
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分子药理学
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药物代谢
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环境毒理学
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蛋白质科学
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DOI
高影响因子
ANALYST
·
2024
·
IF 3.6
Xiaoqing Zhang,Shanglin Li,Mei Liu
Bacterial contamination is a serious issue for public health and food safety. In this work, a simple and label-free fluorescence detection nanoplatform for Escherichia coli (E. coli) was established on the basis of the competitive relationship for the reduction of Cu2+ in CuS-BSA between E. coli and O-phenylenediamine (OPD). OPD could be directly oxidized by Cu2+ to produce 2,3-diaminophenazine (ox OPD) with fluorescence properties. When OPD was introduced into an aqueous solution containing CuS-BSA and E. coli, the oxidation of OPD was inhibited owing to the reduction of Cu2+ to Cu+/Cu0 by NADH-2 dehydrogenase in the bacterial copper homeostasis mechanism, thus decreasing the fluorescence response signal of the system. Meanwhile, our strategy exhibited a satisfactory performance with a broad linear response to E. coli ranging from 12 to 1.2 × 107 CFU mL−1, and the limit of detection was 9 CFU mL−1. The practicability of the developed fluorescence biosensing platform in real samples was evaluated by successful determination of E. coli in drinking water and orange juice. These findings provide a new sensing strategy for analyzing other foodborne bacteria and ensuring food safety assessment.
研究领域:
生物传感
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食品安全
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微生物学
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产品关联
DOI
PubMed
高影响因子
Acta Biomaterialia
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2012
·
IF 4.865
H.-J. Shen,H. Shi,K. Ma,M. Xie,L.-L. Tang,S. Shen,B. Li,X.-S. Wang,Y. Jin
Polyelectrolyte multilayer capsules, promising candidates for multifunctional drug delivery systems, have recently received increased interest. However, the low encapsulation efficiency of drugs and the lack of reports about animal experiments have greatly slowed down their development for drug delivery. Here, a polyelectrolyte multilayer capsule filled with bovine serum albumin gel (BSA-gel-capsule) was constructed by a layer-by-layer assembly technique and thermally induced gelation of BSA. Owing to the charge variability of BSA with change in pH, BSA-gel-capsules not only showed a pronounced accumulation effect of drugs into capsules, but also displayed excellent pH-controlled loading and release properties. Moreover, a remarkable targeting action to the lung was discovered after intravenous injection of fluorescein isothiocyanate (FITC)-labeled BSA-gel-capsules into mice. After treatment with doxorubicin-loaded BSA-gel-capsules, effective cytotoxicity against B16-F10 cells and inhibition of the pulmonary melanoma growth were revealed. This paper introduces a new type of smart microstructure with notable pH-responsive ability. This material renders feasible the intravenous administration of polyelectrolyte microcapsules, which will be a big step towards their application as drug delivery vehicles.
研究领域:
药剂学
·
生物材料
·
肿瘤学
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产品关联
DOI
PubMed
高影响因子
SENSORS
·
2023
·Vol. 23
·期 6
·3209
·
IF 3.9
Yang-Yang Li,Xin-Xin Ma,Xin-Yan Song,Lin-Lin Ma,Yu-Ying Li,Xin Meng,Yu-Jie Chen,Ke-Xin Xu,Ali Akbar Moosavi-Movahedi,Bao-Lin Xiao,Jun Hong
Glucose sensors based blood glucose detection are of great significance for the diagnosis and treatment of diabetes because diabetes has aroused wide concern in the world. In this study, bovine serum albumin (BSA) was used to cross-link glucose oxidase (GOD) on a glassy carbon electrode (GCE) modified by a composite of hydroxy fullerene (HFs) and multi-walled carbon nanotubes (MWCNTs) and protected with a glutaraldehyde (GLA)/Nafion (NF) composite membrane to prepare a novel glucose biosensor. The modified materials were analyzed by UV-visible spectroscopy (UV-vis), transmission electron microscopy (TEM), and cyclic voltammetry (CV). The prepared MWCNTs-HFs composite has excellent conductivity, the addition of BSA regulates MWCNTs-HFs hydrophobicity and biocompatibility, and better immobilizes GOD on MWCNTs-HFs. MWCNTs-BSA-HFs plays a synergistic role in the electrochemical response to glucose. The biosensor shows high sensitivity (167 μA·mM−1·cm−2), wide calibration range (0.01–3.5 mM), and low detection limit (17 μM). The apparent Michaelis–Menten constantKmappis 119 μM. Additionally, the proposed biosensor has good selectivity and excellent storage stability (120 days). The practicability of the biosensor was evaluated in real plasma samples, and the recovery rate was satisfactory.Keywords:glucose oxidase;biosensor;hydroxyl fullerenes;multi-walled carbon nanotubes;bovine serum albumin
研究领域:
生物化学
·
医学诊断
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材料科学
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产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）