Advanced Healthcare Materials
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2019
·Vol. 8
·期 13
·1900435
·
IF 6.27
Luanluan Jia,Fengxuan Han,Huili Yang,Gareth Turnbull,Jiayuan Wang,Jon Clarke,Wenmiao Shu,Mingyu Guo,Bin Li
Nature has created many perfect helical microstructures, including DNA, collagen fibrils, and helical blood vessels, to achieve unique physiological functions. While previous studies have developed a number of microfabrication strategies, the preparation of complex helical structures and cell-laden helical structures for biomimetic applications remains challenging. In this study, a one-step microfluidics-based methodology is presented for preparing complex helical hydrogel microfibers and cell-laden helical hydrogel microfibers. Several types of complex helical structures, including multilayer helical microfibers and superhelical hollow microfibers with helical channels, are prepared by simply tuning the flow rates or modifying the geometry of microfluidic device. With the decent perfusability, the hollow microfibers may simulate the structural characteristics of helical blood vessels and create swirling blood flow in a blood-vessel-on-chip setup. Such hydrogel-based helical microstructures may potentially be used in areas such as blood vessel tissue engineering, organ-on-chips, drug screening, and biological actuators.
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ANALYTICAL CHEMISTRY
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2019
·
IF 6.35
Zhaoyan Tian,Li Mi,Yafeng Wu,Fengying Shao,Mingqiang Zou,Zhenxian Zhou,Songqin Liu
This work reports an electrofluorochromic strategy on the basis of electric field control of fluorescent signal generation on bipolar electrodes (BPEs) for visualizing cancer cell surface glycoprotein (mucin 1). The device included two separate cells: anodic sensing cell and cathodic reporting cell, which were connected by a screen-printing electrode patterned on poly(ethylene terephthalate) (PET) membrane. In the sensing cell, anti-MUC1 antibody immobilized on a chitosan-multiwalled carbon nanotube (CS-MWCNT)-modified anodic BPE channel was used for capturing mucin-1 (MUC1) or MCF-7 cancer cells. Then ferrocene (Fc)-labeled mucin 1 aptamers were introduced through hybridization. Under an applied voltage, the ferrocene was oxidized and the electroactive molecules of 1,4-benzoquinone (BQ) in the cathodic reporting cell were reduced according to electroneutrality. This produced a strongly basic 1,4-benzoquinone anion radical (BQ•–), which turned on the fluorescence of pH-responsive fluorescent molecules of (2-(2-(4-hydroxystyryl)-6-methyl-4H-pyran-4-ylidene)malononitrile) (SPM) coexisting in the cathode reporting cell for both spectrophotometric detection and imaging. This strategy allowed sensitive detection of MUC1 at a concentration down to 10 fM and was capable of detecting a minimum of three MCF-7 cells. Furthermore, the amount of MUC1 on MCF-7 cells was calculated to be 6.02 × 104 molecules/cell. Our strategy also had the advantages of high temporal and spatial resolution, short response time, and high luminous contrast and is of great significance for human health and the promotion of life science development.
研究领域:
生物化学
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生物医学工程
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分析化学
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癌症研究
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JOURNAL OF CHROMATOGRAPHY A
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2012
·
IF 4.531
Xiu-Li Wang,Ying Zhu,Qun Fang
In this work, we developed a microfluidic chip-based liquid chromatography (LC) system with valveless gated sample injection method and monolithic columns. The valveless LC system consisted of a microchip with a separation column and a balance column, two syringe pumps and a laser induced fluorescence detector. Nanoliter-scale sample injection was achieved by simply switching the ON and OFF states of the syringe pumps under gated injection mode, without the need of any mechanical valve. The balance column facilitated the gated injection process by balancing the back pressure in the sample channel with that in the separation channel. Under the gated injection mode, the sample injection volumes could be varied in the range of 0.4–2.4 nL. We investigated the effects of the mobile-phase composition, flow rate , and the sample injection volume on the performance of the LC system. Under the optimized conditions, two fluorescently labeled amines were successfully separated in less than 220 s with a column length of 1 cm. Theoretical plates ranging from 35,700 to 29,400 per meter, corresponding to plate heights ranging from 28 to 34 μm were obtained. The advantages of the present system include simple system structure, ease of operation, convenience for varying injection volume, and high sample loading capacity.
研究领域:
分析化学
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生物工程
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CHINESE JOURNAL OF CHEMISTRY
·
2023
·
IF 5.4
Yin Bao,Kaiwei Cai,Yichen Liu,Bingling Li
Comprehensive Summary The development of accurate, rapid and practical electrochemical sensing technology for antibiotic detection has an important application prospect in many fields such as food safety, environmental monitoring, medical and health care. In this research, kanamycin specific DNA aptamer and its partially complementary short chain were self-assembled onto the surface of an integrated portable plastic gold electrode (PGE) by Au—S bond, and a simple kanamycin electrochemical biosensor interface was constructed. At the same time, the epoxy resin channel layer was designed, and the auxiliary instrument was set up independently in the laboratory, which could help to measure and analyze eight groups of samples automatically in turn. The quantitative analysis results showed that in 20 min, the sensor had a good linear relationship between the peak current change of square wave voltammetry and the negative logarithm of kanamycin concentration when kanamycin concentration was in the range of 1—1000 μmol/L, and the detection limit could reach 0.40 μmol/L. In addition, the discrimination could be achieved within 5 min in the real-time analysis mode. The sensor had a simple construction method, good selectivity and stability, and could be used for rapid or real-time detection of kanamycin residues in actual water samples, which provided a new direction for the practical detection of kanamycin in environmental water samples.
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Micromachines
·
2017
·Vol. 8
·期 7
·218
·
IF 1.833
Shuting Xie,Jun Wu,Biao Tang,Guofu Zhou,Mingliang Jin,Lingling Shui
One of the key fabrication steps of large-area microfluidic devices is the flexible-to-hard sheet alignment and pre-bonding. In this work, the vacuum airbag laminator (VAL) which is commonly used for liquid crystal display (LCD) production has been applied for large-area microfluidic device fabrication. A straightforward, efficient, and low-cost method has been achieved for 400 × 500 mm2microfluidic device fabrication. VAL provides the advantages of precise alignment and lamination without bubbles. Thermal treatment has been applied to achieve strong PDMS–glass and PDMS–PDMS bonding with maximum breakup pressure of 739 kPa, which is comparable to interference-assisted thermal bonding method. The fabricated 152 × 152 mm2microfluidic chip has been successfully applied for droplet generation and splitting.Keywords:large-area;microfluidic devices;fabrication;vacuum airbag laminator
研究领域:
生物工程
·
微纳米技术
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TALANTA
·
2018
·
IF 4.244
Xi Su,Yi Xu,Huazhou Zhao,Shunbo Li,Li Chen
A microfluidic SERS chip integrated with blood separation and in-situ detection was designed and fabricated for the rapid detection of clinical blood samples. Each functional unit in the microfluidic SERS chip consist of separation-decantation cavity based on centrifugal separation principle, mixing channels and SERS detection chamber built with integrated nano-Au on Ag film microstructure. The serum creatinine was selected as a typical sample to demonstrate the capability of microfluidic SERS chip. It was found that the creatinine SERS characteristic peaks at 678 cm −1 can be effectively identified and the detection limit could be as low as 4.42 × 10 −3 μmol mL −1 in water. The blood samples were also tested in microfluidic SERS chip. The whole separation and test process could be completed within 2 min, which is a significant improvement in the field of creatinine detection. The whole blood of six cases clinical blood samples were also tested, and the results were consistent with the enzymatic results. The developed microfluidic SERS chip has advantages including reduction of the required quantity of blood sample, reusable and easy to operate. It is expected to provide a new method for rapid diagnostics .
研究领域:
生物医学工程
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分析化学
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ANALYTICAL BIOCHEMISTRY
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2013
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IF 2.582
Qin Zhang,Xiuwen Tang,Fenghua Hou,Jianping Yang,Zhiyong Xie,Zhiyi Cheng
We fabricated a three-layer polydimethylsiloxane (PDMS)-based microfluidic chip for realizing urease inhibition assay with sensitive fluorescence detection. Procedures such as sample prehandling, enzyme reaction , reagent mixing, fluorescence derivatization , and detection can be readily carried out. Urease reactors were prepared by adsorption of rabbit immunoglobulin G (IgG) and immunoreaction with urease-conjugated goat anti-rabbit IgG. Acetohydroxamic acid (AHA) as a competitive inhibitor of urease was tested on the chip. Microfluidically generated gradient concentrations of AHA with substrate (urea) were loaded into urease reactors. After incubation, the produced ammonia was transported out of reactors and then reacted with o -phthalaldehyde (OPA) to generate fluorescent products. Urease inhibition was indicated by a decrease in fluorescence signal detected by microplate reader. The IC 50 value of AHA was determined and showed good agreement with that obtained in microplate. The presented device combines several steps of the analytical process with advantages of low reagent consumption, reduced analysis time, and ease of manipulation. This microfluidic approach can be extended to the screening of inhibitory compounds in drug discovery.
研究领域:
生物化学
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药物开发
·
分析化学
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ANALYTICA CHIMICA ACTA
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2014
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IF 4.517
Bin Deng,Yu Tian,Xu Yu,Jian Song,Feng Guo,Yuxiu Xiao,Zhiling Zhang
A novel microfluidic chip with simple design, easy fabrication and low cost, coupled with high-sensitive laser induced fluorescence detection, was developed to provide continuous single-cell analysis based on dynamic cell manipulation in flowing streams. Making use of laminar flows, which formed in microchannels, single cells were aligned and continuously introduced into the sample channel and then detection channel in the chip. In order to rapidly lyse the moving cells and completely transport cellular contents into the detection channel, the angle of the side-flow channels, the asymmetric design of the channels, and the number, shape and layout of micro-obstacles were optimized for effectively redistributing and mixing the laminar flows of single cells suspension, cell lysing reagent and detection buffer. The optimized microfluidic chip was an asymmetric structure of three microchannels, with three microcylinders at the proper positions in the intersections of channels. The microchip was evaluated by detection of anticancer drug doxorubicin (DOX) uptake and membrane surface P-glycoprotein (P-gp) expression in single leukemia K562 cells. An average throughput of 6–8 cells min −1 was achieved. The detection results showed the cellular heterogeneity in DOX uptake and surface P-gp expression within K562 cells. Our researches demonstrated the feasibility and simplicity of the newly developed microfluidic chip for chemical single-cell analysis.
研究领域:
生物技术
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分析化学
·
医学研究
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ANALYTICAL CHEMISTRY
·
2016
·
IF 5.886
Huai-Rong Zhang,Yin-Zhu Wang,Wei Zhao,Jing-Juan Xu,Hong-Yuan Chen
In this work, we developed a visual electrochemiluminescence (ECL) sensing platform based on a dual-bipolar electrode (D-BPE) array chip. The chip was composed of two arrays of BPEs and three separated arrays of reservoirs filled with buffer, Ru(bpy)32+-TPrA and luminol solutions, respectively. Both BPEs served as ECL reporting platforms. By applying 6.0 V voltage, an array of orange electrochemiluminescence (ECL) signals belonging to Ru(bpy)32+ turned on. After adding DNAzyme and H2O2 in Ru(bpy)32+ and luminol reservoirs, the orange Ru(bpy)32+ signals decreased until vanished due to the quenching effect; meanwhile, a new array of blue ECL signals turned on because of the luminol-H2O2 ECL reaction. The designed D-BPE owns superior properties compared with the three-electrode system benefiting from the quantitative relation of bipolar systems, which greatly enhanced the ECL detection sensitivity. Meanwhile, the visual color-switch ECL and the ratiometric detecting principle made the results easier to evaluate and more accurate. Quantitative detection of HL-60 cancer cells from 320 cells/mL to 2.5 × 105 cells/mL with two linear ranges was achieved. The detection limit was down to 80 cells/mL. Finally, this D-BPE chip could distinguish the tumor cells from normal cells and provide a prospect for cancer diagnosis in a high-throughput, visual way.
研究领域:
生物传感
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医学诊断
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分析化学
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Materials Today Nano
·
2023
·
IF 10.3
Hongqiang Chen,Xiang Ma,Yonghai Zhang,Jinjia Wei,Paolo Di Marco
Enhancing flow boiling in microchannel via surface modification is crucial for addressing the energy consumption challenges posed by high-power compact electronic devices. However, improving boiling heat transfer performance with well-defined nanostructured surfaces in a limited space remains a challenge. Herein, we present a simple and straightforward acoustofluidics strategy for stable, controllable, and efficient fabricates of functional Zinc oxide (ZnO) nanoarray silicon chip surface with excellent phase change cooling performance. The intentionally designed flower-like sharp-edge structure integrated acoustic has been experimentally and numerically verified for its enhanced mass transfer mixing. The resulting ZnO nanoarray-coated chip with customizable lengths, densities, and morphology is implemented by simple reactor parameter adjustment. Excellent boiling heat transfer performance is obtained on this surface, giving priority to nucleation (superheat≈ 4 °C), low energy consumption (≤3.2 kPa) and simultaneously enhancing the critical heat flux (CHF) and heat-transfer coefficient (HTC) by up to 70.8 % and 107.5 %, respectively, compared with a smooth chip surface. In situ observation and analysis of the wicking of the nanoarray and nucleation, growth, and departure of the bubbles reflect that ZnO nanoarray promotes the phase change heat exchange process by the large number of nucleation sites and ultrafast liquid re-wetting. These findings not only provide important guidelines for the precise control and rational design of functional nanomaterials , but also provide new insights for embedded cooling and significant energy savings on power devices.
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ANALYST
·
2023
·Vol. 148
·期 14
·3295-3305
·
IF 4.2
Yong Huang,Zhengqing Liu,Xiaogang Qin,Jia Liu,Yan Yang,Wei Wei
Highly sensitive protein biomarker detection is critical for the diagnosis of gastric cancer (GC), however the accurate and sensitive detection of low-abundance proteins in early-stage GC is still a challenge. Herein, a surface-enhanced Raman scattering frequency shift assay was performed on a developed microfluidic chip for the detection of GC protein biomarkers carcinoembryonic antigen (CEA) and vascular endothelial growth factor (VEGF). The chip is made up of three groups of parallel channels and each parallel channel consists of two reaction regions, enabling the simultaneous analysis of multiple biomarkers in multiple samples. The presence of CEA and VEGF in the sample can be captured by the 4-mercaptobenzoic acid (4-MBA)-conjugated antibody functionalized gold nano-sheet (GNS-) substrate, resulting in the Raman frequency shift. As a result, a typical Raman frequency shift of 4-MBA presented a linear relationship with the concentration of CEA and VEGF. The limit of detection (LOD) of the proposed SERS microfluidic chip reaches as low as 0.38 pg mL−1 for CEA and 0.82 pg mL−1 for VEGF. During the detection process, only one step of sample addition is involved, which eliminates the multiple reaction step-induced nonspecific adsorption and significantly increases the convenience and specificity. In addition, serum samples from GC patients and healthy subjects were tested and the results were in good agreement with the current gold-standard method ELISA, suggesting the potential application of the SERS microfluidic chip in clinical settings for early diagnosis and prognosis of GC.
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Microsystems & Nanoengineering
·
2023
·Vol. 9
·期 1
·1-14
·
IF 7.9
Li Shibo,Li Chengpan,Khan Muhammad Imran,Liu Jing,Shi Zhengdi,Gao Dayong,Qiu Bensheng,Ding Weiping
Hepatic sinusoids play a key role in maintaining high activities of liver cells in the hepatic acinus. However, the construction of hepatic sinusoids has always been a challenge for liver chips, especially for large-scale liver microsystems. Herein, we report an approach for the construction of hepatic sinusoids. In this approach, hepatic sinusoids are formed by demolding a self-developed microneedle array from a photocurable cell-loaded matrix in a large-scale liver-acinus-chip microsystem with a designed dual blood supply. Primary sinusoids formed by demolded microneedles and spontaneously self-organized secondary sinusoids can be clearly observed. Benefiting from significantly enhanced interstitial flows by formed hepatic sinusoids, cell viability is witnessed to be considerably high, liver microstructure formation occurs, and hepatocyte metabolism is enhanced. In addition, this study preliminarily demonstrates the effects of the resulting oxygen and glucose gradients on hepatocyte functions and the application of the chip in drug testing. This work paves the way for the biofabrication of fully functionalized large-scale liver bioreactors.
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