TRANSITION METAL CHEMISTRY
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2014
·Vol. 39
·期 5
·577-584
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IF 1.402
Li Xin,Bi Cai-Feng,Fan Yu-Hua,Zhang Xia,Wei Xiao-Dan,Meng Xiang-Min
A ternary coordination complex 2Cu(C 14 H 8 NO 3 Cl)(C 12 H 8 N 2 )·3CH 3 OH of a Schiff base (C 14 H 8 NO 3 Cl: 4-chloroanthranilic acid–salicylaldehyde) was synthesized from 4-chloroanthranilic acid, salicylaldehyde, copper acetate and 1,10-phenanthroline and characterized by physicochemical and spectroscopic methods. X-ray crystallography shows that the copper atom is five coordinated by the imine nitrogen, two nitrogen atoms from 1,10-phenanthroline, the hydroxyl oxygen and the carboxylate oxygen atom. The interaction of the complex with calf-thymus DNA was investigated by UV absorption spectroscopy, fluorescence emission spectroscopy and viscosity. In addition, the cleavage reaction with pBR322 supercoiled plasmid DNA was investigated. The complex binds to DNA and also exhibits significant DNA cleavage activity.
研究领域:
生物无机化学
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分子生物学
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药物化学
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化学生物学
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核酸化学
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DOI
高影响因子
JOURNAL OF CRYSTAL GROWTH
·
2024
·
IF 1.7
Sibo Zhao,Dan Xiu,Min Zhang,Yao Wang,Miao Qiu,Christopher D. Snow,Yanxin Wang,Laurence A. Belfiore,Jianguo Tang
In this study, a highly sensitive anticancer drug sensor was synthesized using DNA crystals loaded with luminescent Ln 3+ (Eu 3+ /Tb 3+ ) complexes. The sensors exhibited a pronounced color transformation property due to the differential quenching of Eu 3+ and Tb 3+ emissions upon drug interactions. These luminescent hybrid DNA crystals present a significant advancement in sensing anticancer drugs like curcumin, transitioning from monitoring fluorescence intensity changes to a visually discernable color change. This new approach eliminates the need for specialized instrumentation. Owing to the intrinsic biocompatibility of materials composed of DNA, this hybrid sensor has the potential to sense drugs within the human body. Hybrid crystals loaded with a single complex will lose their fluorescence properties due to drug quenching; however, crystals loaded with mixed complexes emit distinct fluorescent colors, such as yellow-green and orange-red, at different drug concentrations. This color transformation allows for the direct determination of drug concentration based on crystal color, making the sensing process more simple and easily observable.
研究领域:
生物化学
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医学检测
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材料科学
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产品关联
DOI
高影响因子
JOURNAL OF BIOMOLECULAR STRUCTURE & DYNAMICS
·
2020
·
IF 0
Xue-Wen Liu,Ning-Yi Liu,Yuan-Qing Deng,Shan Wang,Ting Liu,Yu-Cai Tang,Yuan-Dao Chen,Ji-Lin Lu
Ruthenium(II) complexes containing phenazine ring have attracted attention to design as ‘molecular light switches’. In this study, we synthesized two ruthenium complexes containing phenazine ring and studied their abilities to function as DNA intercalators, DNA ‘light switches’, DNA topo I inhibitors, DNA photocleavers and potential antitumor reagents. [Ru(bpy)2(mbipz)](PF6)2 (1) (bpy = 2,2′-bipyridine, mbipz = 2-(4′-methyl-bipyridine-4-yl)-1H-imidazo[4,5-b]phenazine) exhibited off–on type DNA ‘light swtich’ behavior. DNA binding modes of the two complexes were determined as intercalation by using UV–vis spectra, emission spectra, viscosity and molecular docking experiments. DNA photocleavage experimental results showed that the two ruthenium complexes effectively cleave plasmid DNA by producing singlet oxygen. Furthermore, they displayed good topo I inhibition activities. We further found that the two complexes displayed good antitumor activities against Eca-109 cells and A549 cells. The results demonstrated that introduction of phenazine ring will be helpful to design DNA ‘light switch’ based on ruthenium complex.Communicated by Ramaswamy H. Sarma
研究领域:
化学
·
分子生物学
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癌症研究
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生物无机化学
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DOI
PubMed
ANALYST
·
2016
·Vol. 141
·期 20
·5829-5834
·
IF 4.033
Xiangmin Miao,Zongbing Li,Liansheng Ling
An ultrasensitive fluorescent platform for sequence-specific recognition of double-stranded DNA (dsDNA) based on the quenching of gold nanoparticles (AuNPs) to a fluorophore labeled DNA probe was developed. The target dsDNA could hybridize with the loop portion of the molecular beacon (MB) to form a triplex DNA structure and opened the “stem–loop” structure of the MB; such triplex DNA was used as an assistant probe (AP). Meanwhile, a fluorophore labeled DNA-AuNP probe that contained a specific enzyme cleavage site was introduced and its fluorescence signal was efficiently quenched due to the vicinity of the fluorophore to the AuNP surface. Such a DNA-AuNP probe could hybridize with the 5′ stem portion of the MB in the AP to form duplex DNA strands that contained a specific enzyme cleavage site for the nicking enzyme assisted cleavage reaction, and resulted in the release of the fluorophore from the AuNP surface and the recovery of the fluorescence signal. Because the AP remains intact during such a cleavage process, it could be reused to hybridize with the next DNA-AuNP probe and trigger the nicking nuclease assisted signal amplification. Under optimal conditions, a low detection limit of 3.8 pM was obtained for dsDNA detection, and the assay has high sequence specificity for dsDNA detection.
研究领域:
生物化学
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纳米生物学
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分子诊断
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产品关联
DOI
PubMed
高影响因子
POLYMER
·
2023
·
IF 4.6
Jianhan Ye,Xin Lu,Xiaohuan Zhao,Fen Wu,Yuanchen Dong,Qian Shi,Lijin Xu
The large-sized glass nanopipette (GN) allows for an access of large targets and show the benefits of excellent mechanical stability and reproducibility. The nanoscale DNA-decorated multilamellar cholesterol assemblies are introduced into the GN with several nanometers, and could in situ grow into a micrometer-scale aggregates under DNA signal stimulus, developing a simple and sensitive strategy for DNA detection in the micrometer-scale GN. The detection limit of target DNA reaches 2.69 fM in a GN with a diameter of up to ca. 4.76 μm.
研究领域:
纳米技术
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生物物理学
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分析化学
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分子生物学
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产品关联
DOI
高影响因子
Nanoscale
·
2015
·Vol. 7
·期 48
·20749-20756
·
IF 7.394
Siqi Zhang,Kun Wang,Congcong Huang,Ting Sun
Based on the characteristics of magnetic beads and DNA, a simple and universal platform was developed for the integration of multiple logic gates to achieve resettable half adder and half subtractor functions. The signal reporter was composed of a split G-quadruplex DNAzyme and AuNP-surface immobilized molecular beacon molecule. The novel feature of the designed system is that the inputs (split G-quadruplexes) can interact with hairpin-modified Au NPs linked to magnetic particles. Another novel feature is that the logic operations can be reset by heating the output system and by using the magnetic separation of the computing modules. Moreover, the developed half adder and half subtractor are realized on a simple DNA/magnetic bead platform in an enzyme-free system and share a constant threshold setpoint. Due to the diversity and design flexibility of DNA, these investigations may provide a new method for the development of resettable DNA-based arithmetic operations.
研究领域:
生物化学
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纳米技术
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分子计算
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生物工程
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DOI
PubMed
高影响因子
ENVIRONMENTAL SCIENCE & TECHNOLOGY
·
2021
·
IF 9.028
Yingcan Zhao,Jiayi Xu,Xingyu Jiang
Chemically exfoliated MoS2 (ce-MoS2) nanosheets have been widely used in biomedical and environmental fields. Some in vitro studies demonstrated that ce-MoS2 might induce toxicity. However, the understanding of the mechanism of potential toxicity is lacking. In this study, we found that ce-MoS2 could directly induce breakage of double-stranded DNA with or without an external energy input, making it different from other two-dimensional nanomaterials. In a dark environment, the DNA cleavage exhibited a pH-dependent trend due to reactive oxygen species generation under different pH values. Under photoirradiation, DNA cleavage could be enhanced. This study provides insights into the potential environmental risk and toxicity of ce-MoS2 in the aquatic environment.
研究领域:
生物医学
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环境科学
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材料科学
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DOI
PubMed
高影响因子
IEEE SENSORS JOURNAL
·
2022
·Vol. 22
·期 19
·18273-18280
·
IF 4.325
Zhongwei Ma,Changwu Lv,Peng Li,Zhenhong Jia,Jierong Xie
Porous silicon (PSi) layers are prepared on the single-crystal silicon surface by electrochemical etching, and PSi membranes (PSiMs) are fabricated by separating the PSi layers from the single-crystal silicon substrate using high-current pulses. PSiMs have better adsorption and response time than nonseparated PSi layers, making them more suitable for sensing applications. PSi microcavity (PSM) structures are widely used for the detection of biomolecules in biosensors because of their high transmission resonance peaks with narrow full-width at half-maximum (FWHM) in the reflection spectra. However, when compared to PSiMs, the permeability of the PSM on the silicon substrate is low, resulting in a significant reduction in its detection sensitivity. In this study, an efficient self-supported Bragg stacked microcavity (SBSM) structure was prepared for the detection of DNA biomolecules by combining the advantages of PSM and PSiMs. The redshift of the reflectance spectra obtained in the concentration range of 0.1– $0.5 ~\mu \text{M}$ shows a good linear relationship, with a detection limit of 2.2 nM. The detection sensitivity is improved by more than a factor of ten when compared to the PSM structure on the silicon substrate. The novel device allows DNA biomolecules to enter the porous layers more easily, especially the defective layer, thus improving its detection sensitivity.
研究领域:
生物传感器
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材料科学
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分子生物学
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产品关联
DOI
高影响因子
ANALYTICAL CHEMISTRY
·
2019
·
IF 6.35
Jianguo Xu,Chao Yan,Xinxin Wang,Bangben Yao,Jianfeng Lu,Guodong Liu,Wei Chen
Facile and efficient assembly of intelligent DNA nano-objects with the ability to exert robust microRNA determination is essential for DNA nanobiotechnology and basic biomedical study. Herein, we present a novel target-triggered DNA assembly pathway for the construction of multibranched DNA nanoarchitectures by the combination of DNA concatamers with G-quadruplex wires. The DNA concatamers acting as the main body structures were assembled by hybridization chain reaction (HCR), while the G-quadruplex wires acting as the branch structures were generated by terminal deoxynucleotidyl transferase (TdT)-promoted polymerization and G-quadruplex units (GUs) based self-assembly. With a copious number of G-quadruplex replicates collected on the electrode surface, the obtained multibranched DNA nanoarchitectures were able to bind with lots of hemin, which further led to a significantly amplified electrochemical sensing signal for ultrasensitive and selective detection of miRNA-21. The detection limit was achieved as low as 0.2 fM with a wide dynamic response ranging from 10 fM to 100 nM, which is much more powerful or at least comparable to most of the reported studies. Furthermore, the electrochemical biosensor offered an excellent specificity, and even the single-base mutation against the perfectly matched target miRNA can be easily distinguished easily. Furthermore, the real biological samples were also desirably analyzed, indicating that the proposed strategy is an ideal platform for the fabrication of versatile DNA nanobiosensors.
研究领域:
DNA纳米生物技术
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生物医学研究
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分子诊断
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产品关联
DOI
PubMed
高影响因子
ACS Omega
·
2023
·
IF 4.1
Yujie Song,Xiao Xie,Yang Liu,Zhen Zhu,Litao Sun
Metal ions are indispensable constituent elements of the human body, among which Cu2+ plays an important role in various biochemical reactions in the human body and is an essential element for maintaining human health. Studying the interaction between Cu2+ and DNA can be helpful to further understand the mechanism of Cu2+ behavior in organisms. In this paper, we investigated the DNA–Cu2+ complex by transmission electron microscopy (TEM) and used in situ liquid-cell TEM to observe the dynamic processes of interactions between DNA and Cu2+. Results show that the binding of Cu2+ to DNA leads to the bending of the DNA strand and provides an anchor site for activating Cu2+ for the nucleation and growth of copper crystals. Bound by the DNA strand, the copper crystals are arranged along the curved strand, showing the same arrangement pattern as guanine on the DNA sequence. It is believed that the study will further elaborate the interaction mechanism by directly observing the DNA–Cu2+ complex at the nanometer scale and benefit the related biomedical research studies.
研究领域:
生物物理学
·
纳米生物学
·
医学研究
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产品关联
DOI
PubMed
高影响因子
Oncotarget
·
2020
·
IF 0
Rahaba Marima,Rodney Hull,Zodwa Dlamini,Clement Penny
The cell-cycle related genes are potential gene targets in understanding the effects of efavirenz (EFV) in lung cancer. The present study aimed at investigating the expression changes of cell-cycle related genes in response to EFV drug treatment in human non-small cell lung carcinoma (A549) and normal lung fibroblast (MRC-5) cells. The loss in nuclear integrity in response to EFV was detected by 4′, 6-diamidino-2-phenylindole (DAPI) staining. Gene expression profiling was performed using human cell cycle PathwayFinder RT 2 Profiler™ PCR Array. The expression changes of 84 genes key to the cell cycle pathway in humans following EFV treatment was examined. The R 2 PCR Array analysis revealed a change in expression of selected gene targets (including MAD2L2, CASP3, AURKB). This change in gene expression was at least a two-fold between test (EFV treated) and the control. RT-qPCR confirmed the PCR array data. In addition to this, the ATM signaling pathway was shown to be upregulated following EFV treatment in MRC-5 cells. In particular, ATM’s upstream activation resulted in p53 upregulation in normal lung fibroblasts. Interestingly, the p53 signaling pathway was activated irrespective of the repressed ATM pathway in A549 cells as revealed by the Ingenuity Pathway Analysis (IPA). These EFV effects are similar to those of ionizing radiation and this suggests that EFV has anti-tumour properties.
研究领域:
肿瘤学
·
分子生物学
·
药理学
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DOI
PubMed
JOURNAL OF CHROMATOGRAPHY A
·
2020
·
IF 4.049
Chunli Lou,Xiongzhen Ye,Ge Chen,Jidong Zhu,Jingwu Kang
Epigenetic inheritance in mammals relies in part on propagation of DNA methylation patterns throughout development. UHRF1 (ubiquitin-like containing PHD and RING finger domains 1) is required for maintenance the methylation pattern. It was reported that UHRF1 is overexpressed in a number of cancer types, and its depletion has been established to inhibit growth and invasion of cancer cells. It has been considered as a new therapeutic target for cancer. In the present work, we described a method for screening inhibitors for blocking the formation of UHRF1–methylated DNA (mDNA) complex by using nonequilibrium capillary electrophoresis of the equilibrium mixture (NECEEM). A recombinant UHRF1 with the SRA domain (residues 408-643), a fluorescently labeled double strand mDNA (12 mer) and a known inhibitor mitoxantrone were employed for proof of concept. The method allows to measure the dissociation constant (K d ) of the UHRF1–mDNA complex as well as the rate kinetic constant for complex formation (k on ) and dissociation (k off ). A small chemical library composed of 60 natural compounds were used to validate the method. Sample pooling strategy was employed to improve the screening throughput. The merit of the method was confirmed by the discovery of two natural products proanthocyanidins and baicalein as the new inhibitors for blocking the formation of UHRF1–mDNA complex. Our work demonstrated that CE represents a straightforward and robust technique for studying UHRF1–mDNA interaction and screening of the inhibitors.
研究领域:
分子生物学
·
癌症研究
·
药物化学
·
生物物理学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）