ADVANCED FUNCTIONAL MATERIALS
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2018
·Vol. 28
·期 10
·1705876
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IF 13.325
Fengyun Li,Chunyan Wang,Weiwei Guo
The development of highly efficient, recyclable, and multifunctional biocatalysts is of great importance for various applications, especially in biosensing. In this study, highly catalytic and recyclable DNAzyme functionalized poly- N -isopropylacrylamide (pNIPAM) microgels are prepared via one-step precipitation polymerization. The pNIPAM/DNAzyme microgels exhibit highly catalytic activities in aqueous solution at room temperature, and become hydrophobic and separable from the reaction mixture at temperature higher than the lower critical solution temperature of pNIPAM, which facilitate the recyclable utilization of these catalysts. Different kinds of DNAzyme functionalized catalytic microgels can be facilely prepared via the one-step synthesis procedure. Two typical catalytic DNA structures, the Mg 2+ -dependent DNAzyme and the hemin-G-quadruplex horseradish peroxidase (HRP)-mimicking DNAzyme, are chosen as model systems to validate the feasibility. These pNIPAM/DNAzyme microgel catalysts maintain 80% to 91% initial catalytic activity after eight times of catalysis recycling. Furthermore, the pNIPAM microgels by themselves provide additional interfaces to capturing an enzyme, glucose oxidase, which can cascade with the linked HRP mimicking DNAzymes, to form recyclable bi-enzyme cascading system for the sensing of glucose.
研究领域:
材料科学
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生物技术
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分析化学
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生物传感器
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高影响因子
BIOSENSORS & BIOELECTRONICS
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2013
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IF 5.437
Yanli Hao,Qingquan Guo,Hanyin Wu,Liangqia Guo,Liangshuang Zhong,Jing Wang,Tianran Lin,FengFu Fu,Guonan Chen
An amplified colorimetric detection of Hg 2+ is proposed by combining T–Hg 2+ –T base pairs and hybridization chain reaction (HCR). Two hairpins consisting of three-fourths and one-fourth of the horseradish peroxidase (HRP)-mimicking DNAzyme in inactive configuration are used as functional elements. In the presence of Hg 2+ , one of the hairpins is opened by an assistant probe with the help of T–Hg 2+ –T base pairs and this triggers an autonomous cross-opening of the two hairpins using the strand displacement principle, resulting in the formation of DNA nanowires consisting of numerous reunited Q-quadruplex DNAzyme units. The resulting catalytically active hemin/G-quadruplex HRP-mimicking DNAzymes catalyze the oxidation of ABTS 2− by H 2 O 2 into a green-colored cationic radical ABTS •+ for the colorimetric readout. This “turn-on” sensing system enables the high sensitive and selective detection of aqueous Hg 2+ with a detection limit of 9.7 pM.
研究领域:
分析化学
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纳米技术
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环境科学
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DOI
PubMed
高影响因子
FOOD CONTROL
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2024
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IF 5.6
Jiong Liang,Yunzhe Zhang,Zikun Li,Xin Lu,Congyan Qi,Qian Yang,Wei Zhang
Aflatoxin B1 is a potent carcinogen widely found in agricultural products and feed. Therefore, investigating the development of convenient, sensitive, and precise techniques for detecting AFB1 is critical. This work proposes a ratiometric electrochemical aptasensor based on DNAzyme-driven tripedal DNA walker for enzyme-free AFB1 detection. The design of multiple legs reduces the moving time on the electrode surface and enhances the value of signal response. In this approach, the tagging of ferrocene and methylene blue as signal switch molecules diminishes the impact of interferences and significantly enhances the reproducibility (RSD: 2.825%). Meanwhile, the tripedal DNA walker and DNAzyme dual signal amplification enabled the sensor to detect AFB1 traces in cereals. We achieved trace detection of AFB1 with a detection limit of 61 fg/mL and a wide linear range from 0.0001 to 10 ng/mL. Furthermore, the aptasensor success rate was comparable to that of HPLC-PMP. The results demonstrate enormous potential for detection. Furthermore, the method can detect various targets by altering the target aptamer , displaying attractive scalability. This suggests its tremendous prospective in detecting mycotoxins in food in the future.
研究领域:
食品安全检测
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生物传感技术
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分子诊断
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DOI
高影响因子
ANALYTICA CHIMICA ACTA
·
2022
·
IF 6.911
Jingjing Li,Zhixue Zhu,Manru Zhang,Qingxin Zhang,Wenyu Sun,Yeru Wang,Long Jiang,Zhiqiang Guo,Yu Wang,Su Liu,Jiadong Huang,Jinghua Yu
Uracil-DNA glycosylase (UDG) is a common glycosylase that can expressly recognize and remove damaged uracil bases, and the ultrasensitive detection of which is significant to maintain genomic stability and early clinical diagnosis of disease. Herein, we proposed a sensitive colorimetric sensing platform to detect UDG. Combined with target-manipulated drawstring DNAzyme and [email protected] nanorods ( [email protected] NRs) indicator, we achieved in naked-eyes observation and ultrasensitive detection of UDG. Briefly, when the UDG exists, the dynamic reaction of rope pulling will occur generating the active conformation of DNAzyme. The cutting effect will be further produced when we add Mg 2+ , thus the generated trigger chain can mediate the occurrence of CHA reaction, followed by generating amount of ·OH which can etch [email protected] NRs causing the shifted of localized surface plasmon resonance (LSPR) peak. By contrast, there is no obvious shift of LSPR peak. This strategy shows extraordinary specificity and sensitivity toward UDG providing a detection limit of 4.6 × 10 −5 U mL −1 . By using of this method, we detected UDG specifically in complex samples, proving that it’s potential applications in biomedical research and clinical diagnosis are fantastic.
研究领域:
生物化学
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纳米技术
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生物医学工程
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临床诊断
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DOI
PubMed
高影响因子
ANALYTICAL CHEMISTRY
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2022
·
IF 8.008
Jiaxin Su,Jinya Du,Rujiao Ge,Chenyang Sun,Yuchun Qiao,Wei Wei,Xuejiao Pang,Yufan Zhang,Huiting Lu,Haifeng Dong
DNAzyme shows great promise in designing a highly sensitive and specific sensing platform; however, the low cellular uptake efficiency, instability, and especially the insufficient cofactor supply inhibit the intracellular molecule sensor applications. Herein, we demonstrate a novel type of DNAzyme-based self-driven intracellular sensor for microRNA (miRNA) detection in living cells. The sensor consists of a metal–organic framework [zeolite imidazole framework (ZIF-8)] core loaded with a shell consisting of a rationally designed DNAzyme, where the substrate strand is modified with FAM and BHQ-1 nearby both the sides of the restriction site, respectively, while the enzyme strand consists of two separate strands with a complementary fragment to the substrate strand and the targeting miRNA, respectively. The ZIF-8 nanoparticles enable the efficient delivery of DNAzyme into the cell and protect the DNAzyme from degradation. The pH-responsive ZIF-8 degradation is accompanied with the release of the DNAzyme and Zn2+ cofactors, and the intracellular target miRNAs recognize and activate the DNAzyme driven by the Zn2+ cofactors to cleave the substrate strand, resulting in the release of the FAM-labeled shorter product strand and increased fluorescence for miRNA detection. The self-driven approach can be generally applied to various miRNAs’ detection through DNAzyme engineering.
研究领域:
生物化学
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分子生物学
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纳米技术
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生物医学工程
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分析化学
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DOI
PubMed
高影响因子
ANALYTICAL CHEMISTRY
·
2022
·
IF 8.008
Xing Huang,Yanfei Zhang,Jun Chen,Lang Zhang,Yuzhi Xu,Wen Yin,Yakun Shi,Si-Yang Liu,Xiaoyong Zou,Zong Dai
Imaging of tumor-associated microRNAs (miRNAs) can provide abundant information for cancer diagnosis, whereas the occurrence of trace amounts of miRNAs in normal cells inevitably causes an undesired false-positive signal in the discrimination of cancer cells during miRNA imaging. In this study, we propose a dual-locked (D-locked) platform consisting of the enzyme/miRNA-D-locked DNAzyme sensor and the honeycomb MnO2 nanosponge (hMNS) nanocarrier for highly specific cancer cell imaging. For a proof-of-concept demonstration, apurinic/apyrimidinic endonuclease 1 (APE1) and miR-21 were chosen as key models. The hMNS nanocarrier can efficiently release the D-locked DNAzyme sensor in living cells due to the decomposition of hMNS by glutathione, which can also supply Mn2+ for DNAzyme cleavage. Ascribing to the smart design of the D-locked DNAzyme sensor, the fluorescence signal can only be generated by the synergistic response of APE1 and miR-21 that are overexpressed in cancer cells. Compared with the miRNA single-locked DNAzyme sensor and the small-molecule (ATP)/miRNA D-locked DNAzyme sensor, the proposed enzyme (APE1)/miRNA D-locked DNAzyme sensor exhibited 2.6-fold and 2.4-fold higher discrimination ratio (Fcancer/Fnormal) for cancer cell discrimination, respectively. Owing to the superior performance, the D-locked strategy can selectively generate a fluorescence signal in cancer cells, facilitating accurate discrimination of cancer both in vitro and in vivo. Furthermore, this D-locked platform is easily adaptable toward other target molecules by redesigning the DNA sequences. The outstanding performance and expansibility of this D-locked platform holds promising prospects for cancer diagnosis and related biomedical applications.
研究领域:
生物化学
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分子生物学
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癌症诊断
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DOI
PubMed
高影响因子
BIOSENSORS & BIOELECTRONICS
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2023
·
IF 12.6
Xing Huang,Zihao Li,Yakun Shi,Yanfei Zhang,Taorong Shen,Meng Chen,Zhan Huang,Yanli Tong,Si-Yang Liu,Jianhe Guo,Xiaoyong Zou,Zong Dai
Autocatalytic biocircuit are powerful tools for analysing intracellular biomarkers, but these tools are constrained by limitations in amplification capacity and intracellular delivery efficiency. In this work, we developed a DNAzyme-based dual-feedback autocatalytic exponential amplification biocircuit sustained by a honeycomb MnO 2 nanosponge (EDA 2 @hMNS) for live-cell imaging of intracellular low-abundance microRNAs (miRNA). The EDA 2 biocircuit comprises a blocked DNAzyme ( b -DNAzyme), a Fuel strand and a Substrate strand. In the EDA 2 biocircuit, target miRNAs are recycled and feedback for rounds of DNAzymatic amplification, and the DNAzymatic reactions continuously generate target miRNA analogues for dual-feedback to achieve multiple parallel cascade DNAzymatic reactions that improve amplification capacity substantially. In addition, the hMNS ensures high loading and delivery efficiency of biocircuit probes into living cells and also provides sufficient Mn 2+ DNAzyme cofactor from in situ decomposition by intracellular glutathione (GSH). The EDA 2 @hMNS realized a detection limit of 17 pM, which is 288-fold lower than the b -DNAzyme lacking the DNAzymatic amplification. These results demonstrate the great promise for this critical tool in analysing low-abundance biomarkers and cancer diagnostics.
研究领域:
分子生物学
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生物化学
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癌症诊断
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DOI
PubMed
高影响因子
MICROCHEMICAL JOURNAL
·
2024
·
IF 4.9
Manlin Luo,Chengyan Yin,Wei Liu,Kerage Dorothy Mokeira,Meng Luo,Ling Tang,Jing Liu,Shuxia Xu
In this study, a Ce13d DNAzyme-based electrochemical biosensor for the detection of sodium ions (Na + ) in the serum was developed. The indium tin oxide (ITO) electrode was modified with Au gold nanoparticles (AuNPs), then immobilized with double-stranded DNA formed by thiol of substrate strand and enzyme strand bound via Au-S link. In the presence of Na + , the resulted Ce13d DNAzyme could preferentially bind Na + , and induced local folding of Ce13d to form a G-quadruplex-like structure, which enhanced binding ability to methylene blue (MB). By using the specificity of DNAzyme and change of its conformation, the quantitative detection of Na + could be achieved. Thus, the developed sensing platform could identify Na + with a linear response range of 10 nM-100 mM and a detection limit of 1.03 nM. The electrochemical biosensor also exhibited excellent selectivity for Na + in the presence of five interfering ions, including K + , Cu 2+ , Mg 2+ , Zn 2+ and Ca 2+ . Furthermore, the proposed biosensor showed satisfactory results for Na + in serum sample analysis, which highly correlated with the inductively coupled plasma atomic emission spectrometry method. This work demonstrated a simple and reliable method for Na + in serum, which opens the way for potential applications in disease diagnosis and environmental testing.
研究领域:
生物化学
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医学检测
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分析化学
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DOI
高影响因子
ACS Applied Materials & Interfaces
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2024
·
IF 8.3
Yinbo Ban,Fu Zhou,Hao Wang,Fuqiang Zhang,Mengmeng Xia,Yifei Wan,Suwan Yang,Rong Liu,Xiayan Wang,Guangfeng Wang
Endowing current artificial chemical reactions (ACRs) with high specificity and intricate activation capabilities is crucial for expanding their applications in accurate bioimaging within living cells. However, most of the reported ACR-based evaluations relied on either single biomarker stimuli or dual activators without obvious biological relevance, still limiting their accuracy and fidelity. Herein, taking the metal-ion-dependent DNAzyme cleavage reaction as a model ACR, two regulators, glutathione (GSH) and telomerase (TE) activated DNAzyme cleavage reactions, were exploited for precise discrimination of cancerous cells from normal cells. DNA probe was self-assembled into the ZIF-90 nanoparticle framework to construct coordination-driven nanoprobes. This approach enhances the stability and specificity of tumor imaging by utilizing biomarkers associated with rapid tumor proliferation and those commonly overexpressed in tumors. In conclusion, the research not only paves the way for new perspectives in cell biology and pathology studies but also lays a solid foundation for the advancement of biomedical imaging and disease diagnostic technologies.
研究领域:
生物医学工程
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分子生物学
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癌症研究
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纳米技术
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细胞生物学
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疾病诊断
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产品关联
DOI
PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2024
·
IF 8
Huinan Chen,Jingyao Song,Yuanyuan Li,Dongmei Deng,Yuchen Song,Xiaoli Zhu,Liqiang Luo
Tumor biomarkers are a kind of important biomolecules for aiding cancer diagnosis and assessing tumor efficacy, among which alpha-fetoprotein (AFP) is a crucial tumor biomarker for the early diagnosis of hepatocellular carcinoma. In this study, a novel cascade signal amplifying strategy was designed for the ultrasensitive detection of AFP by DNAzyme cleaving-mediated hybridization chain reaction (HCR). The primary antibody was immobilized on the Au nanoparticles modified glassy carbon electrode, which subsequently captured AFP and the secondary antibody. Then, streptavidin was combined with the biotin-modified secondary antibody and Cu 2+ -specific DNAzyme. When Cu 2+ was present, the substrate strand of the DNAzyme would cleave and produce an ssDNA end. Finally, HCR was triggered to form long double-stranded DNA on the electrode, which would greatly inhibit the charge transfer at the electrode/solution interface. Therefore, the DNAzyme cleaving-mediated HCR strategy can be applied as cascade signal amplification for the ultrasensitive electrochemical determination of tumor biomarker AFP. Under optimal conditions, the immunosensor exhibits a broad linear range of 0.001−100 ng·mL −1 (R 2 = 0.990) and a low detection limit of 15.8 fg·mL −1 . The proposed sensor displays high sensitivity and selectivity for AFP detection, which can be employed as a universal sensing platform in clinical diagnostics.
研究领域:
生物医学工程
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分析化学
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癌症诊断
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产品关联
DOI
高影响因子
ANALYTICAL CHEMISTRY
·
2022
·
IF 8.008
Yun Chen,Dehui Qiu,Xiaobo Zhang,Yuan Liu,Mingpan Cheng,Jianping Lei,Jean-Louis Mergny,Huangxian Ju,Jun Zhou
G-quadruplex/hemin (G4/hemin) DNAzymes are biosensing systems, but their application remains limited by an overall low activity and a rather high level of unwarranted background reactions. Here, these issues were addressed through the rational design of F3T-azaC-hemin, a G4-based construct in which the hemin is covalently linked to the G4 core and its binding site flanked with a nucleotide activator, here d(T-azaC). This design led to a G4-DNAzyme whose performances have been ca. 150-fold increased compared to the parent G4-based system. The utility of F3T-azaC-hemin was demonstrated here through the ultrasensitive chemiluminescent detection of miRNA-221. The limit of detection (LOD) has been decreased to the femtomolar range, making it a new and highly efficient molecular tool in the biosensing technology field.
研究领域:
生物传感技术
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分子生物学
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分析化学
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DOI
PubMed
高影响因子
ANALYTICA CHIMICA ACTA
·
2026
·
IF 6.1
Daqi Chen,Zhezhi Fang,Limin Wang,Tao Zou
Ultrasensitive and field-deployable detection of Pb 2+ is highly desirable for environmental monitoring and public health protection. Nucleic acid-based sensors are promising for this purpose because they are programmable and compatible with isothermal analysis. However, current amplification strategies face a fundamental trade-off: enzyme-assisted methods provide high sensitivity yet require unstable protein catalysts and cold-chain logistics, whereas enzyme-free circuits are operationally robust but suffer from thermodynamic background leakage. To address this sensitivity–stability paradox, we propose PADLOCK (Pb 2+ -Activated Dual-DNAzyme Locked On-site Cascade Kit), a protein-enzyme-free amplification strategy operating at ambient temperature (25 °C). PADLOCK uses a two-stage catalytic cascade for high-gain signal amplification. Specifically, the upstream GR-5 DNAzyme module was optimized by tuning hairpin stem complementarity and loop geometry to suppress premature trigger release, while the downstream self-locked DNAzyme (SLD) was redesigned through base-pair engineering to improve the signal-to-noise ratio. This cascaded “cleavage-then-unlock” architecture achieves amplification gains substantially exceeding those of conventional enzyme-free circuits such as catalytic hairpin assembly (CHA) or hybridization chain reaction (HCR), while maintaining near-zero background, a combination that is difficult to achieve in enzyme-free systems. Under optimal conditions, PADLOCK demonstrates a sensitivity range of 1 pM to 1 μM with a detection limit of 342 fM and exceptional selectivity over eight competing ions. Reliable quantification in real water and soil samples yielded spike recoveries of 98.17%–111.69%. Furthermore, integration with a custom-built smartphone imaging interface further enables visual monitoring across 100 pM–1 μM, providing a robust, point-of-care testing (POCT) platform that eliminates cold-chain logistics and enables ambient-temperature operation for decentralized heavy-metal screening in resource-limited settings.
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高影响因子
危险品化学品经营许可证（带存储）