FITOTERAPIA
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2023
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IF 3.4
Yang Liu,Limei Ran,Yahong Wang,Peng Wan,Hongli Zhou
The polysaccharides from Sea buckthorn leaves (SBLPs) were extracted by hot water and purified by DEAE cellulose , then separated into six polysaccharides (SBLP-S) by DEAE-52 column. Six separated polysaccharides were characterized by Ultraviolet Spectroscopy, Infrared Spectrum, High Performance Liquid Chromatographic and Congo red analysis. The antioxidant activity and immunological activity were investigated in vitro . The results revealed that the monosaccharide composition of SBLP-S-1, SBLP-S-2, SBLP-S-3, SBLP-S-5 and SBLP-S-6 contained Man , GlcN , Rib , Rha , GluA , GalA , Glu, Gal, Xyl , Ara and Fuc , among them, rare glucosamine was found. And SBLP-S-4 contained all above components except GlcN and GluA. FT-IR showed that SBLP-S were sulfated polysaccharide containing uronic acid . Molecular weights of SBLP-S were 338.659, 401.305, 599.849, 393.904, 626.895 and 176.862 kDa. The Congo-red test indicated that SBLP-S-2, SBLP-S-4, SBLP-S-5, and SBLP-S-6 had triple helix conformation. Crude polysaccharides had the strong scavenging activities on DPPH radicals, ABTS radicals and hydroxyl radicals . The six polysaccharides had the activity of immune stimulation on RAW264.7 cell. SBLP-S-2 promoted the phagocytosis best and SBLP-S-6 promoted the NO production best. The results suggested that SBLPs could be used as potential antioxidants and immunomodulatory agents in pharmaceutical and functional food fields.
研究领域:
药理学
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食品科学
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生物化学
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产品关联
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2011
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IF 2.502
Jianbo Xiao,Jianglei Huo,Huixian Jiang,Fan Yang
The chemical compositions and bioactivities of crude tea polysaccharides (TPS) from the out-of-date tea leaves (beyond their useful date), namely Xihu Longjing (XTPS), Anxi Tieguanyin (TTPS), Chawentianxia (CTPS) and Huizhoulvcha (HTPS), in market were investigated. These TPS showed similar neutral sugar content and different distribution of molecular weight (1–800 kD). These crude TPS were mainly composed of rhamnose, arabinose, galactose, glucose, xylose, mannose, and galacturonic acid. IR spectra confirmed that these crude TPS were composed of polysaccharide, protein and uronic acids. These TPS showed similar DPPH scavenging activity and exhibited lower DPPH scavenging activities than Vc within 25–200 μg/mL. However, these TPS with higher concentrations (200–400 μg/mL) showed similar DPPH scavenging activity with Vc. HTPS exhibited significant higher superoxide anion scavenging activity than others TPS and gallic acid. XTPS showed significant higher inhibitory effects on α-glucosidase and α-amylase with inhibitory percentages of 64.35% and 82.24% than others TPS. TTPS, XTPS, and HTPS exhibited similar inhibition ability on α- d -glucosidase and α-amylase. The overdue tea leaves can be a resource of tea polysaccharides as function food.
研究领域:
食品科学
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营养学
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药理学
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农业化学
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DOI
PubMed
高影响因子
CyTA-Journal of Food
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2013
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IF 0.5
Xin-Huai Zhao,Yu Fu,Nan Yue
Casein hydrolysate of a degree of hydrolysis of 9.4% was generated from caseinate using papain and modified by plastein reaction with extrinsic phenylalanine or tyrosine addition, and then fractionated by an ethanol-water solvent (6:4, v/v). Compared to casein hydrolysate or mixtures of casein hydrolysate and phenylalanine or tyrosine, two modified hydrolysates prepared thereof showed higher scavenging activity on 2,2-diphenyl-1-picrylhydrazyl (DPPH) or hydroxyl radicals and reducing power. The obtained soluble and precipitated fractions had higher and lower total hydrophobic amino acids especially stronger and weaker three antioxidant properties than the parent substrates, respectively. All hydrolysates particularly soluble fractions protected rat hepatocytes against H2O2- or galactosamine-induced injury in vitro, resulting in enhanced cell viability, decreased cellular malondialdehyde content, and lactate dehydrogenase leakage. Cytoprotection of the hydrolysates was observed at a dosage-dependence manner. A negative correlation (P < 0.05) was found between malondialdehyde content and scavenging activity on hydroxyl radicals or reducing power of the hydrolysates.
研究领域:
食品科学
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生物化学
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药理学
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细胞生物学
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药物化学
✓ 已验证
DOI
高影响因子
Food Bioscience
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2024
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IF 4.8
Xiaoli Zhou,Yumeng Qin,Yanli Wang,Yaping Wang,Zhicheng Qin
Rosa roxburghii Tratt (RRT) is renowned for its abundance of phenolics, various methods have been used to extract free phenolics from RRT, there remains a paucity of research on the comparative distinctions between bound phenolic compounds and their free counterparts in RRT, especially in vitro and in vivo antioxidant potential of RRT-BP necessitates further exploration. This study sought to optimize the process parameters for extracting bound polyphenols through alkaline hydrolysis . Among the bound phenolic compounds, 20 phenolic acids and 34 flavonoids were identified, while the free phenolic compounds comprised 19 phenolic acids and 42 flavonoids. The IC 50 values for DPPH and ABTS radical scavenging were 44.92 μg/mL and 60.92 μg/mL for free phenols , 87.02 μg/mL and 95.02 μg/mL for bound phenols, respectively. Compared with the control group, the lifespan of Caenorhabditis elegans fed with 1 mg/mL of free and bound phenol extended from 11.63 d to 16.77 d and 13.88 d, respectively, with no adverse impact on the reproductive capability of the nematodes. The longevity of C. elegans was observed to persist even under thermal and oxidative stress , primarily attributed to elevated superoxide dismutase and catalase activity, coupled with reduced malondialdehyde content in C. elegans . These findings underscore that free polyphenols exhibit superior polyphenolic components and antioxidant properties compared to their bound counterparts. Nevertheless, the potential health effects of bound polyphenols as a supplement to free polyphenols in RRT should not be ignored.
研究领域:
药理学
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生物化学
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分子生物学
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营养学
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生物技术
✓ 已验证
DOI
高影响因子
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
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2024
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IF 5.7
Guangkai Wu,Zihan Zhao,Jun Hu,Yue Li,Jianxia Sun,Weibin Bai
The chemical synthesis of anthocyanins, especially delphinidin-3-O-glucoside and petunidin-3-O-glucoside, is preferable due to the challenges associated with their extraction and purification. However, the reported methods for the synthesis are scarce and intricate. Our research focused on exploring a one-step ester-to-ketone process and optimizing the ring formation reaction, simplifying and improving the overall synthesis strategy. Through these attempts, we were able to achieve higher production yields of delphinidin-3-O-glucoside and petunidin-3-O-glucoside. According to the results of DPPH, ABTS, and FRAP, the antioxidant activity of anthocyanins was increased with the number of B ring hydroxyl substituent. Additionally, both delphinidin-3-O-glucoside and petunidin-3-O-glucoside exhibited no cytotoxicity effects, highlighting their potential for safe application in various fields.
研究领域:
化学
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药学
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生物化学
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食品科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Food Quality and Safety
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2024
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IF 3
Zhang Rongbin,Zhou Zhilei,Ji Zhongwei,Ren Qingxi,Liu Shuangping,Xu Yuezheng,Mao Jian
Lees were the major by-product of Huangjiu brewing, and prone to decompose due to abundant protein residues. To convert the lees into high-value peptides with umami and antioxidant difunctional, herein, a one-step hydrolysis method combined with flavourzyme followed by amylase pretreatment was developed and optimized. The target peptide fraction was collected and evaluated after ultrafiltration and ethanol precipitation, then identified by nano-HPLC-MS/MS. The results show that 80U/g amylase and 3500U/g flavourzyme synergistic hydrolysis 50℃ for 3h performed best umami intensity and antioxidant activity. A total of 5266 peptides was identified from the 80% ethanol precipitation fraction, then filtered 7 umami peptides through virtual screening, sensory, and radical scavenging verification. The umami recognition threshold of 7 peptides ranged from 0.38 to 0.66 mmol/L at water. Among them, DPDGW and DNPNW exhibited well DPPH antioxidant ability with IC50 values of 0.6982 and 0.4315 mg/mL, respectively. Additionally, molecular docking studies indicated that all umami peptides tend to interact with the T1R3 receptor through hydrogen bonds and van der Waals forces, involving key residues such as ASN68, SER104, HIS145, SER276, VAL277, GLU301, ALA302, THR305, and HIS387. This research provides a reference for the flavor peptide resource development, oriented towards dual functionality in flavor and health.
研究领域:
食品科学
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生物化学
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肽化学
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风味化学
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产品关联
DOI
高影响因子
INTERNATIONAL DAIRY JOURNAL
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2019
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IF 2.735
Haixia Wang,Jitao Yang,Min Yang,Wei Ji
Maillard reaction products (MRPs) were prepared by reacting both Yak and Holstein casein with different ratios of glucose to casein from 0:1 to 2.5:1. The results showed that the content of intermediate products and browning intensity of the Yak casein-glucose system increased with increasing concentrations of glucose, but were lower than for the Holstein system at the same ratio. With glycosylation, the reducing power and the 2,2-diphenyl-1-picrylhydrazyl radical scavenging activity of both Yak and Holstein caseins were enhanced. With increased glucose content, the reducing power of Yak MRPs increased at the same concentration. The metal chelating activity of Holstein MRPs decreased or was eliminated in the ratio range of 0.5:1 to 2.0:1 g glucose g −1 casein, while that of Yak MRPs were reduced in general. Improvement of the inhibitory activity for Yak casein was lower than that of Holstein casein after glycosylation by glucose.
研究领域:
食品科学
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化学
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生物化学
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产品关联
DOI
高影响因子
FOOD RESEARCH INTERNATIONAL
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2019
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IF 3.579
Xinfeng Zhang,Sijie Zhang,Beibei Gao,Zheng Qian,Jingjing Liu,Shihua Wu,Jinping Si
Recently, the flowers of Dendrobium catenatum ( D. officinale ) have been approved as new food ingredient. This study aimed to investigate the herb-markers and their antioxidant activities in methanolic extracts of D. catenatum flowers, and to establish the quality evaluation methods for raw materials and their products of flower by HPLC. The methanolic extract of 11 strains of D. catenatum flowers were found to contain a high content of total phenol and flavonoids, and they possessed potential antioxidant capacities based on DPPH radical scavenging assay. A total of 21 phenolic herb-markers were selected according to the similarity and principal component analysis of the chromatographic fingerprinting profiles. Their structures were further elucidated by UV, HPLC-DAD-ESI-QTOF-MS/MS and NMR analyses. The identified compounds included 2 phenylpropanoids, 11 C -glycosylflavones and 6 O -glycosylflavones, which could be employed as the indicators for quantitative evaluation of the quality and authenticity of the flowers. Based on the pre-column DPPH/ABTS + -HPLC analysis, the major compounds contributed to the antioxidative activity were identified as 1- O -caffeoyl- β -D-glucoside, rutin and isoquercitrin, all of which, were also the most abundant constituents in the methanolic extract. The results suggest the potential of D. catenatum flowers as a new antioxidant resources for medicinal and food products.
研究领域:
生药学
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分析化学
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食品科学
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药学
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植物学
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产品关联
DOI
PubMed
高影响因子
Journal of Food Measurement and Characterization
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2019
·Vol. 13
·期 4
·2790-2797
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IF 1.415
Han Yi-Tong,Zhao Chen-Chen,Han Jia-Run,Yan Jia-Nan,Du Yi-Nan,Shang Wen-Hui,Wu Hai-Tao
The purpose of the present study was to explore the antioxidant activity of organic extracts from Maillard reaction products (MRPs) of sea cucumber ( Stichopus japonicus ) gut hydrolysates (SCGHs). The SCGHs were obtained after treatment with neutrase, and they were then reacted with ribose at a mass ratio of 1:2, pH 8.0, and 95 °C for up to 12 h. The MRPs were extracted by methanol and ethanol. The methanol extracts of the MRPs had a higher UV absorbance, browning intensity, and fluorescence intensity than the crude MRPs. The scavenging capacities of methanol and ethanol extracts from MRPs in both 2′,2′-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) and 1,1-diphenyl-2-picrylhydrazyl radical (DPPH) assays increased significantly ( p < 0.05). The methanol extracts were better than the ethanol extracts showing the reduced IC 50 levels for ABTS and DPPH by 50% and 50%, respectively. The inhibitory activity for peroxidation of linoleic acid of MRPs was also enhanced in the methanol extracts of MRPs, but this was reduced in ethanol extracts. Moreover, HepG2 cell viability after exposure to the methanol extracts of MRPs was increased to 59.30 ± 0.43% compared with the SCGHs and MRPs, which was 3.97 times that of the H 2 O 2 -treated group. These results suggest that methanol extraction can improve the antioxidant activity of SCGHs-ribose MRPs and that the corresponding extracts can be potentially used as food antioxidants.
研究领域:
食品科学
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营养学
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生物化学
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药理学
✓ 已验证
产品关联
DOI
高影响因子
Journal of Functional Foods
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2016
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IF 3.973
Meng-Hua Liu,Chun-Hay Ko,Na Ma,Pei-Wen Tan,Wei-Ming Fu,Jing-Yu He
Bambusa textilis McClure ( Bambusoideae ) is a valuable bamboo species widely distributed in southern China. The methanolic extract of B. textilis leaves was examined for its chemical and bioactive properties. As a result, 38 compounds, including organic acids, flavonoids and miscellaneous substances, were identified or tentatively characterised by UFLC-DAD-Q-TOF-MS. Thirteen polyphenols, considered as the most important components, were quantified using LC-MS/MS method. Moreover, the methanolic extract of B. textilis leaves had obvious antioxidant and anti-obesity activities in vivo in a high-fat diet rat model. It also had a significant antioxidant effect in 2,2-diphenyl-1-picrylhydrazyl (DPPH), ferric reducing antioxidant power (FRAP) and inhibition of β-carotene bleaching assays in vitro . These results are useful for further research of B . textilis leaves that may be useful candidates for the development as a functional food ingredient in health-related industries.
研究领域:
药理学
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营养学
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生物化学
✓ 已验证
产品关联
DOI
高影响因子
Frontiers in Sustainable Food Systems
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2022
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IF 5.005
Dong Yuhe,Zhong Wenting,Yang Chunmiao,Zhang Yongze,Yang Dongsheng
The objectives of this study are to optimize the ultrasonic microwave synergistic extraction (UMSE) of anthocyanins from Lycium ruthenicum Murr (ALRM) by response surface methodology and also to investigate its antioxidant activity in vitro. Based on the single-factor experiments, we optimized the ALRM extraction process by response surface methodology assuming anthocyanin extraction rate as the response point and microwave power, ultrasonic power, extraction time, and liquid-to-material ratio as the investigating parameters as well as performed correlation analysis between DPPH·, ·OH, ABTS·, O2-· radical scavenging potential, and ferric-reducing antioxidant power. The results showed that the influence on anthocyanin yield was in the order of liquid to material ratio > microwave power > ultrasonic power > extraction time; the elicited extraction conditions were: ultrasonic power 216.253 W, microwave power 89.311 W, time 26.141 min, liquid to material ratio 17.294 mL/g, the forecast content was 10.157 mg/g, while the actual value was consistent with the preview value. Meanwhile, ALRM obtainable by UMSE was identified as yielding favorable antioxidant properties firstly, when the concentration of purified ALRM (ALRM-1) was increased, the antioxidant capacity was strengthened. Theoretically, we provide a basis for the extraction procedure of ALRM and its antioxidant activity, which serves as a promising antioxidant and free radical scavenger.
研究领域:
食品科学
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植物化学
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分析化学
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营养学
✓ 已验证
产品关联
DOI
高影响因子
Food Packaging and Shelf Life
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2022
·
IF 8.749
Pengquan Zhao,Jiandong Wang,Xinyu Yan,Zihui Cai,Lina Fu,Qi Gu,Lin Liu,Haojie Jin,Yujie Fu
In this study, the bioactive extract of Rosa roxburghii Tratt (RRT) leaves was incorporated into a chitosan/zein matrix to produce a food packaging film with higher antioxidant and antimicrobial activities. The natural deep eutectic solvent (NADES) was taken as the extraction solvent and green plasticizer to prepare the film matrix. The physical and structural properties of chitosan/zein films with different concentrations of RRT leaves extracts were characterized by infrared spectroscopy analysis , thermogravimetric analysis , X-ray diffraction and scanning electron microscope. The incorporation of RRT leaves extract significantly improved the mechanical properties , light barrier properties and water vapor permeability of the chitosan/zein films. Moreover, the films containing RRT leaves extract had better antioxidant activities and antibacterial activities, with the highest DPPH and ABTS radical scavenging activities of 88.17 % and 86.84 %, respectively, and also effectively inhibited the growth of microorganisms and extended the shelf life of blueberries and cherry tomatoes .
研究领域:
食品科学
·
材料科学
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生物技术
·
化学
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）