JOURNAL OF ETHNOPHARMACOLOGY
·
2024
·
IF 4.8
Zhengchao Ji,Beihan Sun,Ting Yang,Xuan Li,Zhenwei Zhang,Mengfan Bao,Liping Zhao,Hongyin Lou,Yanyan Li,Guoxiang Sun,Jing Huang
Ethnopharmacological relevance YiXinShu capsule (YXSC), originally from the classical TCM formula named “Sheng-Mai-San”, has been extensively utilized in clinic for the treatment of cardiovascular diseases. However, there were few reports about the quality assessment of YXSCs both internationally and domestically. Aim of the study The objective was to develop a multi-strategy platform incorporating systematic quantitative fingerprint analysis and antioxidant activity determination, with chemometric analysis and bivariate correlation analysis as the auxiliary approaches, to assess and monitor the quality of YXSCs. Materials and methods Firstly, according to the Chinese Pharmacopoeia (2020 edition), 12 key indicator components from seven herb medicines were quantified by HPLC method. Then, three-dimensional fingerprints comprising five-wavelength fusion fingerprint (FWF-FP), electrochemical fingerprint (EC-FP) and Differential Scanning Calorimetry fingerprint (DSC-FP) were established to assess and monitor YXSCs using systematically quantified fingerprint method (SQFM) and principal component analysis (PCA). Moreover, by integrating the analysis of the three-dimensional fingerprints, the quality of YXSCs from different batches was effectively screened. Finally, the antioxidant activity of this TCM was assessed through DPPH and ABTS methods, and the L-ascorbic acid equivalent antioxidant capacity (AEAC) values were compared to evaluate the antioxidant activities of the two methods. A Partial Least Squares (PLS) model was used to develop the spectrum-activity relationship between FWF-FP and AEAC, and a bivariate correlation analysis (BCA) was used to assess the correlation between FWF-FP and EC-FP. Results The key indexes including tanshinone I, t ol , t oe , A tp , first exothermic peak, and second exothermic peak can differentiate between various batches of YXSCs based on their three-dimensional fingerprint profiles. The integration evaluation results from 42 batches of YXSCs were categorized into 2–5 grades, indicating good quality consistency across different batches. In vitro studies have indicated a significant antioxidant activity capacity of YXSCs. The PLS model revealed that 37 out of the 41 fingerprint peaks exhibited antioxidant activity. The overall trend of BCA was consistent with PLS model results. Conclusion This research presents a scientific and holistic strategy for the quality consistency evaluation of YXSCs, thereby offering an effective approach for the thorough evaluation of TCMs.
研究领域:
药理学
·
中药学
·
分析化学
·
质量控制
·
生物统计学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
MATERIALS CHEMISTRY AND PHYSICS
·
2023
·
IF 4.6
Qianyu Wei,Shuman Feng,Lili Wu
Chronic kidney disease caused by functional impairment of glomerular endothelial cells (GECs) is one of the etiologies of hemodialysis treatment. As a critical component of the hemodialysis process, hemodialysis membranes having been suffering from intricated modification methods. In order to solve a series of problems caused by blood incompatibility, usually triggered by rough surfaces, an anticoagulant coating with a similar physical structure and physiological properties to GECs was prepared on the polyethersulfone (PES) membrane. The smooth glomerular endothelium-like coating was obtained by a hydrated layer formed by tannic acid (TA) and α-lipoic acid (α-LA). We explored two possible reaction mechanisms of TA and α-LA, which were characterized and discussed by ATR-FTIR and XPS. Besides, the hydrophilic and negatively charged coating has filtration and anticoagulant properties similar to those of GECs. The results show that the modified membranes have appreciable hydrophilicity (the water contact angle is 29.7°) as well as high urea removal rate (96.5%) and BSA protein retention (99.7%). In addition, the modified membranes show favorable hemocompatibility and free radical scavenging ability. The reduction of platelet adhesion number is reduced by 98.7% and PRT is prolonged to 297 s. DPPH and ABTS radical scavenging capacity reach 1.18 and 0.89 μmol/cm 2 , respectively. In this work, a surface coating mimicking GECs was fabricated for the potential candidates of new hemodialysis membranes, which will significantly enhance the dialysis efficiency and alleviate the coagulation.
研究领域:
生物医学工程
·
材料科学
·
肾病学
✓ 已验证
DOI
高影响因子
JOURNAL OF CHROMATOGRAPHY A
·
2015
·
IF 4.169
Xiaoxia Zhang,Zongtao Lin,Jinggui Fang,Meixian Liu,Yanyan Niu,Shizhong Chen,Hong Wang
An on-line high-performance liquid chromatography−diode-array detector−electrospray ionization−ion-trap−time-of-flight−mass spectrometry−total antioxidant capacity detection (HPLC−DAD−ESI−IT−TOF−MS−TACD) system was created for identification and evaluation of antioxidants in Prunus ( P. ) mume flowers. Applying this system, the HPLC fingerprint, ultraviolet (UV) spectra, mass fragmentations, active profiles against 1,1-diphenylpicryl-2-hydrazyl radical (DPPH•) scavenging activity and ferric reducing antioxidant power (FRAP) of each complex sample were obtained simultaneously after one injection. Synchronous structure identification and activities screening of complex samples were thus accomplished. In this study, 78 compounds were identified from P. mume flowers by their chromatographic behaviors, UV spectra and MS data with the assistance of standard compounds and literature reports. The DPPH and FRAP activity of 24 samples (23 different P. mume varieties and 1 related herbal medicine) were then quantified by their detailed activity profiles from the on-line system, and by the total activity of each sample extract from off-line 96-well plate method. As a result, 21 and 32 compounds in the on-line system showed anti-oxidative effects against DPPH and FRAP, respectively. The established on-line system is efficient, sensitive and reliable to tell the DPPH and FRAP antioxidant activities of individual compound in complex samples, and therefore would be a useful and promising technique for antioxidant screening from different food and medicinal matrices.
研究领域:
植物化学
·
分析化学
·
食品科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF ETHNOPHARMACOLOGY
·
2014
·
IF 2.939
Ping Xiao,Hongzhi Huang,Jianwei Chen,Xiang Li
Ethnopharmacological relevance Radix Isatidis called “Ban-Lan-Gen” is one of the most commonly-used traditional Chinese medicines for antiviral, anti-inflammatory, antioxidant and antipyretic purposes. Investigate the bioaccessibility of uridine , epigoitrin, adenosine, clemastanin B, indigoticoside A and isolariciresinol as well as the antioxidant and anti-inflammatory activities during an in vitro gastro-intestinal digestion of the Radix Isatidis extract (RIE). Materials and methods High performance liquid chromatography (HPLC) technique was adopted to determine the bioaccessibility of six bioactive compounds in RIE. Antioxidant activities of RIE in different digestive stages were determined by 1,1-Diphenyl-2-picrylhydrazyl (DPPH), superoxide anion and hydroxyl radical scavenging abilities. Anti-inflammatory activity was assayed by the inhibitions of inflammatory cytokines such as nitrous oxide (NO), prostaglandin E 2 (PGE 2 ) and tumor necrosis factor α(TNF-α) producted by lipopolysaccharide (LPS) stimulated RAW264.7 cells. Results The bioaccessibility of uridine, epigoitrin, adenosine, clemastanin B, indigoticoside A and isolariciresinol were 15.38%, 18.28%, 24.01%, 6.50%, 8.65% and 17.78%, respectively. Also, the digestion products still possessed certain antioxidant activities. The antioxidant activity was highly correlated with lignans (clemastanin B, indigoticoside A and isolariciresino). The anti-inflammation activity of the three samples decreased in the order: IN sample (the solution that had diffused into the dialysis tubing)>Nondigested sample (RIE solution)>Gastric sample (post-gastric digestion)>OUT sample (material that remained in the gastro-intestinal tract). Conclusions Results obtained in this research reveal the amount of bioactive compounds from RIE that could be available for absorption in vivo . The antioxidant activity decreased significantly but the anti-inflammatory activity was enhanced in serum-available fraction after gastro-intestinal digestion in vitro . This study could provide a scientific basis for a deeper pharmacological activity study of Radix Isatidis and a simple method for pharmacodynamic material basis research.
研究领域:
药理学
·
民族药理学
·
生物化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2014
·
IF 3.096
Huiling Kong,Jixin Yang,Yifeng Zhang,Yapeng Fang,Katsuyoshi Nishinari,Glyn O. Phillips
Selenium nanoparticles (SeNPs) were prepared by using gum arabic (GA) as the stabilizer in a facile synthetic approach. The size, morphology, stability and antioxidant activity in vitro of the gum arabic-selenium nanocomposites (GA-SeNPs) were characterized by transmission electron microscopy (TEM), dynamic light scattering (DLS), Fourier-transform infrared spectroscopy (FTIR), atomic force microscopy (AFM) and ultraviolet–visible spectrophotometry (UV–vis). SeNPs (particle size of ∼34.9 nm) can be stabilized in gum arabic aqueous solutions for approximately 30 days. FTIR results show that SeNPs were combined to the hydroxyl groups of GA. In the present work, the alkali-hydrolyzed GA (AHGA) was also prepared and its efficiency in stabilizing SeNPs was compared with GA. It was concluded that the branched structure of GA was a significant factor for the functionality. The hydroxyl radical scavenging ability and DPPH scavenging ability of GA-SeNPs were higher than those of AHGA-SeNPs and could reach 85.3 ± 2.6%, 85.3 ± 1.9% at a concentration of 4 mg/ml, respectively.
研究领域:
材料科学
·
纳米技术
·
化学
·
生物技术
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF FOOD BIOCHEMISTRY
·
2016
·Vol. 40
·期 4
·603-612
·
IF 0.832
Lu Zhang,Zong-Cai Tu,Hui Wang,Qing-Hui Wen,Zhi-Feng Fu,Xing Xie
Effects of dynamic high pressure microfluidization (DHPM), microwave, ultrasound, maceration and high temperature, on the phenolic acids and antioxidant activity of Artemisia selengensis Turcz (AST) were investigated. Microwave exhibited the best extraction efficacy on phenolic compounds and flavonoids with the content of 40.30 μg GAE/mg Extract, and 37.75 μg RTE/mg Extract, respectively. Microwave extract (MWE) also showed the strongest DPPH· and HO· scavenging ability (IC 50 value of 0.23 and 0.50 mg/mL, respectively), and reducing power (Ab 0.3 value of 0.23 mg/mL). Quinic acid, 3 caffeoylquinic acids (CQAs), 2 coumaroylquinic acids, 4 dicaffeoylquinic acids (diCQAs) were identified as the major phenolic acids in AST using HPLC-QTOF-MS/MS, and 3-CQA (63.71 μg/mL) showed the highest content followed by 1,5-diCQA (17.91 μg/mL) and 3,5-diCQA (13.50 μg/mL). MWE had the highest 3-CQA and diCQAs. While, high temperature could promote the degradation of diCQAs, 3- and 4-caffeoyl are more liable to decompose as compared with 1- and 5-caffeoyl. Practical Application Over production of reactive oxygen species (ROS) in body will induce various chronic diseases. AST is an underutilized vegetable with many health benefits. This research indicates that AST possessed appreciating antioxidant activities and abundant phenolic acids, it is could be a potentail antioxidants resource and for prevention of chronic diseases associated with ROS. It also suggested that microwave could be a promising method for extracting the antioxidants in AST. Therefore, this research could lay a foundation for the high-value application of AST in food, health care and natural therapy industry, and provide some methodological references for the development of antioxidants in AST.
研究领域:
药理学
·
食品科学
·
分析化学
✓ 已验证
产品关联
DOI
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2024
·
IF 7.7
Yanyan Qiao,Yixiao Shen,Hongzhou Jiang,Dongnan Li,Bin Li
In this study, three pectin polysaccharides BP1, BP2 and BP3, were purified from blueberries. The weight-average molecular weight (Mw) of BP1, BP2, and BP3 were detected to be 9.027 × 10 4 , 9.313 × 10 4 , and 1.223 × 10 6 Da, respectively. The structures of the three pectin polysaccharides were characterized and compared based on the results of molecular weight, monosaccharide composition, GC–MS and NMR analysis. Structural characterization revealed that BP1, BP2, and BP3 all contain homogalacturonan (HG) and rhamnogalacturonan I (RG-I) domains, and the rhamnose residues in RG-I domains are substituted at C-4 with side chains such as araban and galactosan. BP2 had the highest degree of esterification and HG domain ratio, followed by BP3 and BP1. In addition, BP1, BP2 and BP3 showed great antioxidant and antibacterial activities, and could destroy the cell membrane of Staphylococcus aureus and Escherichia coli . Moreover, the better DPPH and ABTS free radical scavenging and antibacterial activities of BP1 and BP2 than BP3 might be related to their lower molecular weight. The results of this study will provide essential information for the structure-activity relationship of pectin polysaccharides and research basis for development and application of blueberry pectin polysaccharides.
研究领域:
食品科学
·
生物化学
·
药理学
✓ 已验证
DOI
PubMed
高影响因子
Food & Function
·
2020
·Vol. 11
·期 6
·5004-5016
·
IF 4.171
Kaixin Guo,Linnan Su,Yongchao Wang,Huihui Liu,Jing Lin,Peng Cheng,Xiquan Yin,Ming Liang,Qiangqiang Wang,Zebo Huang
Sea cucumbers have been used as food delicacies and traditional medicine for centuries, and their health benefits are partly attributed to their repertoire of proteins. Peptides prepared from the sea cucumber Apostichopus japonicus are reported to have in vitro antioxidant activities. Here, we investigated the in vivo antioxidant capacity of AjPH, a peptide-rich A. japonicus protein hydrolyzate, and found that AjPH is capable of increasing the survival rate and reducing the reactive oxygen species (ROS) level in the animal model Caenorhabditis elegans under increased oxidative stress induced by paraquat. AjPH is also shown to enhance the antioxidant defense system in paraquat-exposed nematodes, including upregulation of superoxide dismutase and catalase activities and reduction of malondialdehyde contents. To explore underpinning antioxidant mechanisms, cellular and chemical assays were used to demonstrate that AjPH not only reduces ROS accumulation in cells but also directly scavenges DPPH free radicals. Further studies indicate that AjPH can decrease age pigments and extend lifespan but does not reduce food intake, body length and brood size of the nematodes, demonstrating its capacity to delay physiological aging. Using activity-guided fractionation by ultrafiltration and gel filtration, we then isolated antioxidant fractions from AjPH and identified the sequences of their composing peptides, which were subjected to in silico analysis for prospective motifs, physicochemical properties and antioxidant potential. Taken together, our results provide an insight into the nutraceutical potential of the sea cucumber protein hydrolyzate for aging and related conditions and also a basis for future mechanistic studies of individual antioxidant peptides.
研究领域:
营养保健品
·
生物化学
·
分子生物学
·
药理学
·
衰老研究
✓ 已验证
产品关联
DOI
PubMed
高影响因子
INDUSTRIAL CROPS AND PRODUCTS
·
2024
·
IF 5.6
Xinlin Zhang,Xinyu Yan,Zihui Cai,Lina Fu,Xinyue Dong,Jihong Cui,Hangrong Zheng,Mingyue Xu,Yujie Fu
In the current work, a temperature-responsive system consisting of a fatty acid-based hydrophobic deep eutectic solvent and a choline chloride (ChCl)-based hydrophilic deep eutectic solvent was innovatively developed. It was used to extract and separate polar compounds (chlorogenic acid, rutin, hyperin, isoquercetin, hydroxy-α-sanshool) from Zanthoxylum bungeanum Maxim. peels (ZBMP). Different molar ratios of the hydrophobic deep eutectic solvent (DES) composed of decanoic acid (DA)-octanoic acid (OA) and hydrophilic DES composed of ChCl-levulinic acid (Lev) were chosen as the switching medium. Under the optimal extraction conditions, the TRBDES consisting of hydrophilic DES (ChCl: Lev/1:2): hydrophobic DES (OA: DA/2:1): H 2 O (v/v/v,5/5/1) demonstrated a greater total extraction rate and distributive efficiency than the conventional solvents. High DPPH radical scavenging capacity of target compounds (IC 50 = 0.29 mg/mL) from the bottom phase recovered by D101 macroporous resin. Hydrogels prepared by DES containing extracts in the top phase showed significant inhibition of E. coli (15.5 ± 0.7 mm). and S. aureus (21.0 ±1.4 mm). The solvent morphologies and the intrinsic switching mechanism were observed and illustrated. Moreover, density functional theory (DFT) calculations showed that hydrogen bond production is crucial for extraction. This work provides a resource for exploring and utilizing TRBDES for environmentally friendly extraction of various bioactive active ingredients from plant sources.
研究领域:
化学
·
药学
·
食品科学
·
环境科学
✓ 已验证
产品关联
DOI
高影响因子
Evidence-based Complementary and Alternative Medicine
·
2012
·
IF 4.774
Yuan-yuan Chai,Fang Wang,Yan-li Li,Ke Liu,Hui Xu
Rheum emodi Wall has been reported to possess protective effect in many inflammatory diseases and oxidative stress-related injuries. This study aims to investigate antioxidant power of stilbenoids from R. emodi and then explore the material basis for its antioxidant potential. The most abundant stilbenoid piceatannol-4′-O-β-D-glucopyranoside (PICG) and its aglycon piceatannol (PICE) were isolated from R. emodi rhizome. Using well-accepted antioxidant chemicals as reference, antioxidant activity of these stilbenoids was examined by measuring DPPH and superoxide anion radical scavenging, ferric reducing power, and inhibition of lipid peroxidation in vitro. Both PICG and PICE displayed promising antioxidant activity in all the four assays. Comparisons among the tested compounds indicated that PICE has the most potent antioxidant activity and the presence of 3′-hydroxyl group may enhance antioxidant activity of stilbenoids. The antioxidative effect of PICE at the cellular level was further demonstrated on the model of hydrogen-peroxide-induced H9c2 rat cardiomyoblasts injury. Taking into account the rapid in vivo metabolic transformation of PICG into PICE it can be inferred that the most abundant stilbenoid PICG may be an important constituent responsible for the antioxidant potential of R. emodi and promising to be developed as an antioxidant agent for supplementary or therapeutic use.
研究领域:
药理学
·
生物化学
·
药物化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
EUROPEAN FOOD RESEARCH AND TECHNOLOGY
·
2014
·Vol. 240
·期 1
·49-60
·
IF 1.387
Li Chao,Fu Xiong,Huang Qiang,Luo Faxing,You Lijun
Response surface methodology was used to optimize the ultrasonic extraction of Prunella vulgaris polysaccharides (PVP). When the quality of P. vulgaris was 4.0 g, the optimal extraction conditions were ultrasonic power 210 W, time 50 min, and temperature 70 °C. Using this condition, the experimental yield was agreed closely with the predicted value. Compared with conventional extraction, ultrasonic extraction showed a higher polysaccharide yield. The molecular weights were determined by high-performance gel permeation chromatography. Gas chromatography analysis suggested that PVP comprised of rhamnose, arabinose, xylose, mannose, glucose, and galactose with molar percentages of 2.8, 28.2, 38.5, 11.0, 3.0, and 16.5 %, respectively. The glycosidic linkage analysis showed that PVP contained 1→ and 1→6 glycosidic linkages (48.0 %), 1→2 and 1→4 glycosidic linkages (8.1 %), and 1→3 glycosidic linkages (43.9 %). PVP showed good antioxidant activities by the methods of DPPH radical scavenging assay, oxygen radical absorbance capacity assay, and cellular antioxidant activity assay. The antiproliferative activities of PVP (2.0 mg/mL) on the growth of human hepatoma HepG2, gastric carcinoma SGC 901, and human breast cancer MCF-7 cells were 51.2, 35.2, and 31.8 %, respectively. PVP did not exhibit cytotoxicity against normal liver L02 cells within the tested concentrations. These results demonstrated that polysaccharides isolated from P. vulgaris may be helpful to develop potential antioxidant and antitumor agents for food and pharmaceutical industries.
研究领域:
药理学
·
生物化学
·
食品科学
·
医学
✓ 已验证
产品关联
DOI
高影响因子
Foods
·
2021
·Vol. 10
·期 2
·239
·
IF 4.35
Nina Bao,Di Wang,Xizhe Fu,Hujun Xie,Guizhen Gao,Zisheng Luo
LotusReceptaculum Nelumbinishas been sparking wide research interests due to its rich phenolic compounds. In the present work, ultrasonic-assisted extraction coupled with glycerol was employed to extract phenolic compounds fromReceptaculum Nelumbinisand the process was optimized using a response surface methodology with Box-Behnken design (BBD). The optimal conditions for the total phenolic content (TPC) extract were obtained: glycerol concentration of 40%, an extraction temperature of 66 °C, ultrasonic time of 44 min, and the solvent-to-solid ratio of 55 mL/g. Under these optimum extraction conditions, the extraction yield of TPC was 92.84 ± 2.13 mg gallic acid equivalents (GAE) /g. Besides, the antioxidant activities demonstrated the ability of free radical scavenging by four different methods that included 2,2-Diphenyl-1-picrylhydrazyl (DPPH), ferric reducing antioxidant power (FRAP), 2,2′-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS), and reducing activity (RA) were 459.73 ± 7.07, 529.97 ± 7.30, 907.61 ± 20.28, and 983.66 ± 11.80 μmol TE/g, respectively. Six phenolic compounds were identified by ultra-high pressure liquid chromatography combined with triple-time-of-flight mass spectrophotometry (UPLC-Triple-TOF/MS) from the extracts. Meanwhile, Fourier transform infrared (FTIR) was conducted to identify the characteristic functional groups of the extracts and thus reflected the presence of polyphenols and flavonoids. Scanning electron microscopy (SEM) illustrated the microstructure difference of four treatments, which might explain the relationships between antioxidant activities and the structures of phenolic compounds.
研究领域:
植物化学
·
分析化学
·
食品科学
·
天然产物研究
✓ 已验证
DOI
高影响因子
危险品化学品经营许可证（带存储）