INDUSTRIAL CROPS AND PRODUCTS
·
2015
·
IF 2.837
Shanshan Wang,Dongmei Wang,Zehua Liu
Potentilla fruticosa and EGb 761 ( Ginkgo biloba extracts) have been applied in traditional medicine and exhibit antioxidant properties. However, there is very little scientific evidence to support their use in combination. In this study, a bio-guided chromatographic fractionation strategy was used to obtain two effective fractions (C-3 and D-2) from the acetone crude extracts (A) of P. fruticosa leaves. Isobolographic analysis indicated that the combination of A + EGb had largely demonstrated synergism and 5:1 (A:EGb) displayed the best synergistic effect. In addition, C-3 + EGb exhibited a similar result as A + EGb, synergism in DPPH and ABTS assays, additive in FRAP assay. While D-2 + EGb showed only additive effect. The possible mechanism behind the synergism was explored by RP-HPLC. The changes in the total phenolic and flavonoids content were also investigated. While chemical composition did not seem to change in the mixture, no new peaks appeared in the chromatogram of C-3 + EGb mixture as compared to the individual chromatograms, no existing peaks disappeared either. Further studies were observed which may prove the possible mechanism responsible for the synergistic effect. Combinations of C-3 and 4 phenolic compounds in EGb ((+)-catechin, kaempferide, quercetin and isorhamnetin) were evaluated for their antioxidant capacities. Isorhamnetin showed synergism with C-3, and 5:1 exhibited the strongest synergism in DPPH ( γ = 0.669) and ABTS ( γ = 1.249), 1:1 in FRAP assays ( γ = 1.385). Isorhamnetin was added to the other 6 phenolics in C-3 ((+)-catechin, caffeic acid, hyperoside, rutin, ellagic acid and quercetin), and the antioxidant capacity of mixtures was analyzed. For all combinations studied, caffeic acid + isorhamnetin displayed the greatest synergistic effect in various ratios by three antioxidant assays, the observed values were significantly higher than the expected values ( P < 0.05) and 1:1 exhibited the strongest synergism in DPPH ( γ = 0.711), ABTS ( γ = 1.693) and FRAP ( γ = 1.474) assays. Hyperoside + isorhamnetin also showed a slight synergism, but (+)-catechin + isorhamnetin exhibited an antagonism in DPPH assays. Thus, interaction of phytochemicals exist in extracts is likely responsible for the synergistic effects present in A + EGb, and caffeic acid + isorhamnetin may have played an important role. This study lends some credence to the concomitant use of P. fruticosa blended with EGb, which could be useful for developing new medicines and functional products from P. fruticosa and EGb.
研究领域:
药理学
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生物化学
·
药物化学
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DOI
高影响因子
Food & Function
·
2022
·Vol. 13
·期 11
·6008-6021
·
IF 6.317
Rui Ma,Qiqi Chen,Yanping Dai,Yan Huang,Qing Hou,Yuting Huang,Kai Zhong,Yina Huang,Hong Gao,Qian Bu
Ocean life contains a wealth of bioactive peptides that could be utilized in nutraceuticals and pharmaceuticals. This study aimed to obtain neuroprotective antioxidant peptides in sea squirt (Halocynthia roretzi) through protamex enzymolysis. Fraction F4 (ultrafiltration generated four fractions) had a lower molecular weight (<500 Dalton (Da)) with greater 1,1-diphenyl-2-picrylhydrazyl (DPPH) and 2,2-azino-bis (3-ethylbenzothiazoline-6-sulphonic acid) diammonium salt (ABTS) radical scavenging activities (94.24 ± 2.50% and 91.80 ± 1.19%). After gel filtration, six peptides, including Phe-Gly-Phe (FGF), Leu-Gly-Phe (LGF), Leu-Phe-VAL (LFV), Val-Phe-Leu (VFL), Trp-Leu-Pro (WLP), and Ile-Ser-Trp (ISW), were identified and sequenced by liquid chromatography-mass spectrometry (LC-MS/MS). Peptides WLP and ISW showed higher oxygen radical absorbance capacity (ORAC) values (2.72 ± 0.47 and 1.93 ± 0.01 μmol L−1 of Trolox equivalent (TE) per μmol L−1 of peptide) than glutathione (GSH). Additionally, WLP effectively increased cell viability, dramatically attenuated 6-Hydroxydopamine (6-OHDA)-induced cell apoptosis and decreased reactive oxygen species (ROS) levels to nearly two-fold, and significantly boosted glutathione peroxidase (GSH-Px) activity in PC12 cells. Transcriptome sequencing revealed differential expression of genes associated with various oxidative stress pathways after WLP treatment, such as glutathione metabolism. These results suggest that the Halocynthia roretzi-derived tripeptide WLP could alleviate neurodegenerative diseases associated with oxidative stress.
研究领域:
海洋生物学
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药理学
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神经科学
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生物化学
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产品关联
DOI
PubMed
高影响因子
CARBOHYDRATE POLYMERS
·
2022
·
IF 10.723
Hui Li,Tao Gao,Zhonghao Zhang,Jiangping Lei,Junchao Hu,Zizhong Tang,Shiling Feng,Chunbang Ding,Tao Chen,Yanger Chen,Shu Yuan,Ming Yuan
Polysaccharides were the key ingredients of many herbal medicines, and were responsible for multiple pharmacological activities. In this study, a novel polysaccharide fraction, named SLP-2, was isolated from Stauntonia leucantha fruits, and purified by DEAE-52 and Sephadex G-100 column chromatography . Furthermore, SLP-2 was identified by congo red, methylation , partial acid hydrolysis and NMR . The results indicated that the backbone of SLP-2 was composed of →4)-β-D-Gal p -(1 → 4)-β-D-Gal p -(1→ substituted at C-6 with 1,5-linked arabinan . SLP-2 had good anti-oxidation ability in vitro. Surprisingly, we found that reduction of carboxyl groups and methylation of hydroxyl groups enhanced the ability to scavenge 2,2′-azinobis (3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS) radicals and inhibit lipid peroxidation , and weakened the activity to scavenge 1,1-diphenyl-2-picrylhydrazyl (DPPH) radicals and reduce ferric iron.
研究领域:
药理学
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化学
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植物科学
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生物化学
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产品关联
DOI
PubMed
高影响因子
Chemical and Biological Technologies in Agriculture
·
2023
·Vol. 10
·期 1
·1-18
·
IF 6.6
Biswas Ashok,Dey Susmita,Xiao Aiping,Deng Yong,Birhanie Ziggiju Mesenbet,Roy Rana,Akhter Delara,Liu Liangliang,Li Defang
Background Besides fibre production, Corchorus olitorius is considered a nutritional and medicinal plant in traditional medicine. Its leaves possess different antioxidant compounds and display various biological properties. This study optimized the ultrasound-assisted extraction (UAE) parameters like temperature, time, solvent concentration, and liquid–solid ratio for total flavonoids (TFC) and total polyphenols content (TPC) from leaves employing response surface methodology (RSM). Results Findings demonstrated that under the optimized conditions, the highest extraction yield of total flavonoids (7.17 mg QE/g DW) and total polyphenol content (13.92 mg GAE/g DW) were recorded with the ethanol concentration 70.92%, temperature 68.06 °C, liquid–solid ratio 48.80 mL/mg, and ultrasound irradiation time 37.20 min. The optimum value of TFC (6.96 mg QE/g DW) and TPC (13.38 mg GAE/g DW) from the experiment of verification of optimized conditions was close to the predicted value and significantly superior to the conventional heat reflux extraction (HRE). LC–MS and HPLC analysis of the optimized extract from UAE demonstrated the existence of six major phenolic compounds, including chlorogenic acid, isoquercetin, hyperoside, adhyperforin, 1,3-di- O -caffeoylquinic acid, and 3,4-di- O -caffeoylquinic acid. Furthermore, the antioxidant test of the UAE leaves extract revealed an excellent 2,2-diphenyl-1-picrylhydrazyl (DPPH•), 2,2-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS + •) and hydroxyl radical scavenging with IC 50 values of 226.29, 199.53 and 402.02 µg/mL, respectively, compared to HRE with 336.31 µg/mL, 253.86 µg/mL, 520.08 µg/mL. Conclusions The developed optimization method could contribute to the good recovery of natural antioxidants from C. olitorius in the pharmaceuticals and food industries. Graphical Abstract
研究领域:
药学
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食品科学
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分析化学
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植物学
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产品关联
DOI
高影响因子
Food Bioscience
·
2023
·
IF 5.2
Hongchi Zhou,Sixuan Wang,Weichao Liu,Lixuan Chang,Xuemei Zhu,Guangqing Mu,Fang Qian
This study aimed to investigate the safety and probiotic properties of Lactobacillus paraplantarum LS-5 isolated from traditional northeastern sauerkraut , and to assess the antioxidant capacity of different solvent extracts (ethanol, water, and hot water) of L. paraplantarum LS-5-inoculated sauerkraut. L. paraplantarum LS-5 was shown to have various probiotic characteristics, such as good survival ability under artificial gastrointestinal conditions, antagonistic activity of various pathogens, non-biogenic amines, antibiotic susceptibility , and non-hemolytic. The 2,2′-azino-bis-3-ethylbenzothiazoline-6-sulfonate (ABTS) radical scavenging activity of the L. paraplantarum LS-5-inoculated sauerkraut alcohol extract (71.13%) was significantly higher than water extract (59.59%) ( p < 0.05). The ferric reducing antioxidant power (FRAP) of sauerkraut alcohol extract (614.93 Trolox μmol L −1 ) exhibited a slightly stronger ability compared to water extract (388.53 Trolox μmol L −1 ) and the heated sauerkraut water extract (383.20 Trolox μmol L −1 ) at extract concentrations of 5.0 mg mL −1 . Its 1, 1-diphenyl1-2-picrylhydrazyl (DPPH) radical scavenging activity was also stronger. It was found by principal component analysis (PCA) and Pearson that the DPPH radical-scavenging activities of sauerkraut extracts were significantly correlated with the content of polyphenols and flavonoids ( p < 0.05). Unlike kimchi , Northeastern sauerkraut is consumed mainly by heating, so this study could contribute to understanding the antioxidant capacity of different solvent extracts of sauerkraut, and before and after heating water extracts.
研究领域:
微生物学
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食品科学
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营养学
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生物化学
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产品关联
DOI
高影响因子
CHEMISTRY & BIODIVERSITY
·
2024
·
IF 2.3
Linhong He,Lijuan Zhang,Xi Bai
This study evaluated the effects of three extraction methods, hydrodistillation (HD), steam distillation (SD), and lipophilic solvent extraction (LSE), on the yield, chemical composition, and in vitro / in silico biological activities of Schizonepeta tenuifolia (Benth.) Briq essential oil (STEO). The highest yield of STEO was obtained by HD (0.750±0.040%), followed by SD (0.440±0.020%) and LSE (0.350±0.030%). Although their contents varied, menthone and pulegone predominated in HDEO, SDEO, and LSEO. HDEO displayed the strongest antioxidant ability, with a 2,2-diphenyl-1-picrylhydrazyl (DPPH) IC50 value of 14.164±0.090 mg/mL and 2,2′-azinobis-(3-ethylbenzothiazoline-6-sulfonate) (ABTS) value of 0.326±0.023 mg/mL. SDEO demonstrated the highest antimicrobial activity against Staphylococcus aureus and Escherichia coli and HDEO the highest potent anti-inflammatory activity. The molecular docking of menthone and pulegone demonstrated strong binding to inflammatory targets, including prostaglandin-endoperoxide synthase 1 (PTGS1) and 2 (PTGS2) and tumor necrosis factor alpha (TNF-α). In short, the extraction method significantly affected the yield, composition, and biological activity of STEO.
研究领域:
药用植物学
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精油化学
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药理学
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分子生物学
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产品关联
DOI
PubMed
高影响因子
FOOD SCIENCE AND BIOTECHNOLOGY
·
2023
·
IF 2.9
Wu Jiao,Zhao Shan,Chen Xin,Jiu Yuanda,Liu Junfeng,Gao Jinglin,Wang Shijie
Honey quality is in relation to botanical origin, and physicochemical properties, elemental composition, and antioxidant activity have been used for assessment and identification of honeys. The goal of this study is to contribute to the general analysis of five unifloral honeys from Cocos nucifera L., Dalbergia benthami Prain, Bombax ceiba L., Castanea mollissima Bl., and mangrove in Hainan province, China. Our results revealed that B. ceiba honey had the highest pH (4.27), color (139.33 mm Pfund), ash content (1.03 g/100 g), and electrical conductivity (1312.00 μS/cm) in five unifloral honeys. Furthermore, B. ceiba honey also contained the highest levels of total phenolic content (75.54 mg GAE/100 g) and total flavonoid content (29.22 mg RE/100 g), as well as the strongest antioxidant activity (DPPH IC 50 value, 3.97 mg/mL; FRAP value, 6527.43 µmol TE/kg). Moreover, we revealed a considerable variation in element contents in honeys using ICP-MS, with potassium being the most predominant element. B. ceiba honey had the highest contents of K, Ca, Mg, and P, whereas the highest amount of Na was found in mangrove honey. Overall, our data indicated that B. ceiba honey deserves further research as a potential antioxidant agent.
研究领域:
农业
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食品科学
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分析化学
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生物化学
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产品关联
DOI
PubMed
高影响因子
RSC Advances
·
2016
·Vol. 6
·期 101
·98935-98944
·
IF 3.289
Hongshan Liang,Bin Zhou,Jing Li,Yaqiong Pei,Bin Li
The layer-by-layer (LBL) deposition technique has been widely used to decorate the nanofibers formed from polymer pairs with complementary functional groups. In the current study, an antioxidative coordination-driven multilayer electrospun nanofibrous film was fabricated from tannic acid (TA) and phosvitin (PV) obtained from egg yolk. PV was reported to bond with 95% of yolk iron which could provide chelating sites for TA. The surface morphology of the nanofibrous mats was observed by scanning electron microscopy (SEM). Also the TEM image of the cross-section illustrated a uniform shell formed around the cellulose nanofibers. The deposition of TA and PV was further confirmed by X-ray photoelectron spectroscopy (XPS) and Fourier transform infrared spectroscopy (FT-IR). TA/PV nanofibrous mats showed good 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical-scavenging activity regardless of the outmost component. While for superoxide-scavenging and hydroxyl radical-scavenging activity, the outmost component affected the scavenging capacity of nanofibrous mats. Scanning electron microscopy (SEM), X-ray photoelectron spectroscopy (XPS) and X-ray diffraction (XRD) were combined to characterize the morphology and structure of the deposited mineral phase on the scaffolds after culturing in a simulated body fluid (SBF) for 5 days. From the result, the TA/PV nanofibrous mats were satisfactory for use in bioapplications.
研究领域:
材料科学
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生物材料
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组织工程
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抗氧化研究
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生物医学工程
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产品关联
DOI
高影响因子
JOURNAL OF FOOD BIOCHEMISTRY
·
2022
·Vol. 46
·期 8
·e14192
·
IF 3.654
Hongan Li,Chengyue Zhang,Zeyuan Deng,Bing Zhang,Hongyan Li
The quantum chemical density functional theory and in vitro chemical-based antioxidant assays were used to research the reaction mechanism of delphinidin/pelargonidin with free radicals including superoxide anion radicals (O 2 − ∙) and hydroperoxide radicals (OOH∙). The geometric configuration, bond dissociation energy, PCM (polarizable continuum model) solvent model reaction enthalpy changes were studied to explain the transition states, and the reaction enthalpy change value was calculated to determine the active site. From the results of spatial configuration, delphinidin showed a stronger conjugation effect than that of pelargonidin. The dihedral angle between the three rings of delphinidin was almost 180°, and the angle between the B and C rings was only −2.81868°. Both coplanar and antioxidant activity of delphinidin was better than pelargonidin. The consequences of reaction enthalpy change in PCM were consistent with the bond dissociation energy. The phenolic hydroxyl bond dissociation energy of delphinidin was slightly smaller than that of pelargonidin. Moreover, the C4' site of delphinidin and the C3 site of pelargonidin were the active sites for scavenging free radicals. The free radical scavenging ability of delphinidin was marginally higher than that of pelargonidin. On the other hand, in vitro antioxidant results proved the scavenging ability of delphinidin and pelargonidin on superoxide anions, DPPH, and ABTS ∙+ free radicals. It was shown that the chemical-based antioxidant activity was consistent with the theoretical calculation results, with delphinidin showing greater antioxidant activity. These results could explain the antioxidant mechanism of delphinidin/pelargonidin in scavenging free radicals from chemical reactions. Practical applications This manuscript explained the antioxidant mechanism of delphinidin/pelargonidin in scavenging free radicals through the analysis of the geometric configuration of delphinidin/pelargonidin and the theoretical calculation of the reaction transition state. It could also speculate on the possible reaction sites, and provide a basis for judging how to efficiently select antioxidants with great antioxidant activity.
研究领域:
化学
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生物化学
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分子生物学
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药理学
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产品关联
DOI
PubMed
高影响因子
COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
·
2024
·
IF 4.9
Wei Liu,Lin Wang,Zipei Zhang,Xiaolai Zhang,Jingcheng Hao
As the improvement of consciousness of environment, a considerable effort has been made to shift from petroleum-based plastics to biodegradable alternatives, especially in the food packaging industry. In this work, we develop an innovative food packaging film by encapsulating quercetin (Que) within β -cyclodextrin ( β -CD) to create β -CD-Que inclusion complexes, which are then dispersed in a matrix of tragacanth gum (TG) and carboxymethyl chitosan (CMCS) using a solution casting technique. β -CD-Que inclusion complexes were characterized using various methods, including 1 H NMR, 2D ROESY and phase solubility testing, to elucidate the interaction between Que and β -CD. ATR–IR, X-ray diffraction, and SEM were employed to confirm the uniform dispersion of β -CD-Que within the macromolecular substrate. The incorporation of β -CD-Que considerably improves the mechanical properties of films, increasing the elongation at break from 13.68 % to 25.74 %. Rheological tests demonstrate that the viscosity of the film-forming complex solution became larger. The films were demonstrated to reduced water vapor and oxygen permeability, possessing remarkable antioxidant capability evidenced by DPPH and ABTS radical scavenging rates of 78.01 % and 99.59 %, respectively. The TG/CMCS/ β -CD-Que composite film shows excellent potential for food packaging and preservation applications.
研究领域:
食品科学
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材料科学
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环境科学
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产品关联
DOI
高影响因子
JOURNAL OF DRUG DELIVERY SCIENCE AND TECHNOLOGY
·
2025
·
IF 4.9
Dan Huang,SuQiu Si,Zhuoming Chen,Binjie Xin
In this study, a novel nanofiber material possessing antibacterial and antioxidant properties was fabricated through coaxial electrospinning, utilizing polylactic acid (PLA), thymol (THY), and nano zinc oxide (ZnO) as the raw materials. THY and ZnO was used to endows the membranes with good antibacterial and antioxidant properties. The surface morphology of the PLA/Z@T nanofiber membrane was characterized using scanning electron microscopy (SEM). Additionally, the successful formation of the core-shell structure was verified through transmission electron microscopy (TEM), fluorescence microscopy, and Fourier transform infrared spectroscopy (FTIR). Additionally, both ZnO and THY exhibit strong antibacterial properties, and their synergistic effect significantly enhances the antibacterial activity of the composite nanofiber membranes against Staphylococcus aureus . The drug sustained release percentages within 24 h for PLA@T and PLA/Z@T were 15.91 % and 14.82 %, respectively. The addition of zinc oxide does not significantly affect the release efficiency of thymol. After continuous release for 288 h, these percentages increased to 73.35 % and 72.94 %, respectively. By assessing antioxidant activity through DPPH and ABTS assays, it was found that PLA/Z@T exhibited higher activity compared to the control group. Subsequently, the in vitro antibacterial performance was investigated, revealing an antibacterial rate higher than that of the control group, the antibacterial rates against Staphylococcus and Escherichia coli reached 99.75 % and 98.49 % respectively. In conclusion, the results demonstrate that PLA/Z@T nanofiber membranes possess excellent antibacterial efficiency, sustained antibacterial performance, and antioxidant properties, offering a novel strategy for antibacterial materials.
研究领域:
材料科学
·
纳米技术
·
生物医学工程
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药剂学
·
微生物学
✓ 已验证
产品关联
DOI
高影响因子
LWT-FOOD SCIENCE AND TECHNOLOGY
·
2022
·
IF 6.056
Yidan Zhang,Xu Jing,Zhenjia Chen,Xiaowen Wang
In this paper, the preparation, purification, and identification of the antioxidant peptides of millet gliadin after high hydrostatic pressure (HHP) treatment were studied to broaden the utilization range of millet and provide certain theoretical basis for its further processing and utilization. Millet gliadin treated with 300 MPa was enzymatically hydrolyzed by pepsin, and the optimal hydrolysis time was determined to be 50 min by in-vitro antioxidant activity evaluation method [1,1-diphenyl-2-trinitrophenylhydrazine (DPPH) free radical, hydroxyl radical, and superoxide anion scavenging activity]. The enzymatic hydrolysate was separated and purified by gel filtration and RP-HPLC, and the fraction with the strongest antioxidant activity (S4) was obtained. The scavenging ability of DPPH, •OH, and O 2 − were 53.96% ± 0.86%, 31.30% ± 0.90%, and 52.73% ± 0.99%, respectively. However, it still had lower antioxidant capacity compared with VC. Seven peptide sequences, namely, SLSHLTVQ, SLAHVTVQ, LHALTLQ, SGILAPSPVL, SHLTVQ, VSAAAA, and SPAAF, were identified from S4. The presence of certain amino acids, such as Glycine, Leucine, Phenylalanine, and Proline , is considered to induce high antioxidant activity against lipid peroxidation. Therefore, the antioxidant peptide extracted from millet gliadin after HHP treatment may be used as a natural antioxidant component in functional food.
研究领域:
食品科学
·
生物化学
·
农业加工
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DOI
高影响因子
危险品化学品经营许可证（带存储）